%PDF-1.3 1 0 obj << /Type /Catalog /Outlines 2 0 R /Pages 3 0 R >> endobj 2 0 obj << /Type /Outlines /Count 0 >> endobj 3 0 obj << /Type /Pages /Kids [6 0 R 164 0 R 251 0 R 267 0 R 297 0 R 311 0 R 341 0 R 357 0 R 365 0 R 415 0 R 513 0 R ] /Count 11 /Resources << /ProcSet 4 0 R /Font << /F1 8 0 R /F2 9 0 R /F3 10 0 R /F4 11 0 R /F5 166 0 R >> /XObject << /I1 12 0 R /I2 13 0 R /I3 255 0 R /I4 256 0 R /I5 259 0 R /I6 260 0 R /I7 263 0 R /I8 264 0 R /I9 265 0 R /I10 266 0 R /I11 271 0 R /I12 272 0 R /I13 275 0 R /I14 276 0 R /I15 279 0 R /I16 280 0 R /I17 283 0 R /I18 284 0 R /I19 287 0 R /I20 288 0 R /I21 291 0 R /I22 292 0 R /I23 293 0 R /I24 294 0 R /I25 295 0 R /I26 296 0 R /I27 315 0 R /I28 316 0 R /I29 319 0 R /I30 320 0 R /I31 323 0 R /I32 324 0 R /I33 327 0 R /I34 328 0 R /I35 331 0 R /I36 332 0 R /I37 335 0 R /I38 336 0 R /I39 337 0 R /I40 338 0 R /I41 339 0 R /I42 340 0 R /I43 345 0 R /I44 346 0 R /I45 349 0 R /I46 350 0 R /I47 353 0 R /I48 354 0 R /I49 355 0 R /I50 356 0 R /I51 359 0 R /I52 360 0 R /I53 361 0 R /I54 362 0 R /I55 363 0 R /I56 364 0 R >> >> /MediaBox [0.000 0.000 612.000 792.000] >> endobj 4 0 obj [/PDF /Text /ImageC ] endobj 5 0 obj << /Creator (DOMPDF) /CreationDate (D:20180723041110+00'00') /ModDate (D:20180723041110+00'00') /Title (A mixed fixed ratio/progressive ratio procedure reveals an apathy phenotype in the BAC HD and the z_Q175 KI mouse models of Huntingtons disease PLOS Currents Huntington Disease) >> endobj 6 0 obj << /Type /Page /Parent 3 0 R /Annots [ 14 0 R 16 0 R 18 0 R 20 0 R 22 0 R 24 0 R 26 0 R 28 0 R 30 0 R 32 0 R 34 0 R 36 0 R 38 0 R 40 0 R 42 0 R 44 0 R 46 0 R 48 0 R 50 0 R 52 0 R 54 0 R 56 0 R 58 0 R 60 0 R 62 0 R 64 0 R 66 0 R 68 0 R 70 0 R 72 0 R 74 0 R 76 0 R 78 0 R 80 0 R 82 0 R 84 0 R 86 0 R 88 0 R 90 0 R 92 0 R 94 0 R 96 0 R 98 0 R 100 0 R 102 0 R 104 0 R 106 0 R 108 0 R 110 0 R 112 0 R 114 0 R 116 0 R 118 0 R 120 0 R 122 0 R 124 0 R 126 0 R 128 0 R 130 0 R 132 0 R 134 0 R 136 0 R 138 0 R 140 0 R 142 0 R 144 0 R 146 0 R 148 0 R 150 0 R 152 0 R 154 0 R 156 0 R 158 0 R 160 0 R 162 0 R ] /Contents 7 0 R >> endobj 7 0 obj << /Length 27773 >> stream q 375.000 0 0 39.000 222.000 738.000 cm /I2 Do Q q 15.000 659.406 577.500 78.594 re W n 0.267 0.267 0.267 rg BT 15.000 718.042 Td /F2 21.0 Tf [(A mixed fixed ratio/progressive ratio procedure reveals an )] TJ ET BT 15.000 693.094 Td /F2 21.0 Tf [(apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 15.000 668.146 Td /F2 21.0 Tf [(models of Huntingtons disease)] TJ ET Q 0.271 0.267 0.267 rg BT 15.000 650.140 Td /F3 9.8 Tf [(April 25, 2012)] TJ ET BT 76.483 650.140 Td /F3 9.8 Tf [()] TJ ET 0.267 0.267 0.267 rg BT 81.358 650.140 Td /F3 9.8 Tf [(HD Models)] TJ ET BT 26.250 638.299 Td /F1 9.8 Tf [(Stephen Oakeshott)] TJ ET 0.271 0.267 0.267 rg BT 109.720 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 115.141 638.299 Td /F1 9.8 Tf [(Russell Port)] TJ ET 0.271 0.267 0.267 rg BT 167.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 173.114 638.299 Td /F1 9.8 Tf [(Jane Cummins-Sutphen)] TJ ET 0.271 0.267 0.267 rg BT 277.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 283.114 638.299 Td /F1 9.8 Tf [(Jason Berger)] TJ ET 0.271 0.267 0.267 rg BT 341.097 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 346.518 638.299 Td /F1 9.8 Tf [(Judy Watson-Johnson)] TJ ET 0.271 0.267 0.267 rg BT 442.975 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 448.396 638.299 Td /F1 9.8 Tf [(Sylvie Ramboz)] TJ ET 0.271 0.267 0.267 rg BT 513.409 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 518.830 638.299 Td /F1 9.8 Tf [(Neil Paterson)] TJ ET 0.271 0.267 0.267 rg BT 26.250 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 31.671 626.394 Td /F1 9.8 Tf [(Seung Kwak)] TJ ET 0.271 0.267 0.267 rg BT 86.407 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 91.829 626.394 Td /F1 9.8 Tf [(David Howland)] TJ ET 0.271 0.267 0.267 rg BT 157.387 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 162.808 626.394 Td /F1 9.8 Tf [(Dani Brunner)] TJ ET 0.271 0.267 0.267 rg BT 26.250 614.490 Td /F1 9.8 Tf [(Oakeshott S, Port R, Cummins-Sutphen J, Berger J, Watson-Johnson J, Ramboz S, Paterson N, Kwak S, Howland D, Brunner )] TJ ET BT 26.250 602.585 Td /F1 9.8 Tf [(D. A mixed fixed ratio/progressive ratio procedure reveals an apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 26.250 590.680 Td /F1 9.8 Tf [(models of Huntingtons disease. PLOS Currents Huntington Disease. 2012 Apr 25 . Edition 1. doi: 10.1371/4f972cffe82c0.)] TJ ET q 15.000 33.272 577.500 555.027 re W n 0.271 0.267 0.267 rg BT 26.250 561.578 Td /F4 12.0 Tf [(Abstract)] TJ ET BT 26.250 541.623 Td /F1 9.8 Tf [(Apathy, characterized by generally reduced interest in and likelihood to perform goal-directed actions, is a recognized symptom )] TJ ET BT 26.250 529.719 Td /F1 9.8 Tf [(of Huntingtons disease \(HD\), a devastating neurological disorder caused by a CAG repeat expansion of the Htt gene located on )] TJ ET BT 26.250 517.814 Td /F1 9.8 Tf [(chromosome 4. The present experiments used a modified progressive ratio task that incorporated a fixed-ratio schedule of )] TJ ET BT 26.250 505.909 Td /F1 9.8 Tf [(reinforcement component to assess consummatory behavior, and a progressive-ratio schedule component that required )] TJ ET BT 26.250 494.004 Td /F1 9.8 Tf [(increasing numbers of lever-presses for successive reinforcers \(0.01 ml of evaporated milk\). The studies revealed an apathetic )] TJ ET BT 26.250 482.100 Td /F1 9.8 Tf [(phenotype in two mouse models of HD, with decreased response rates either overall or only at higher ratio requirements in the )] TJ ET BT 26.250 470.195 Td /F1 9.8 Tf [(progressive-ratio component relative to wild-type controls. Based on the procedure used \(within-session fixed- and progressive-)] TJ ET BT 26.250 458.290 Td /F1 9.8 Tf [(ratio components\), it is proposed that an observed phenotype can be ascribed either specifically to reduced motivation to work )] TJ ET BT 26.250 446.385 Td /F1 9.8 Tf [(for food reinforcement or more generally to deficits in consummatory behavior. This procedure provides a simple means to )] TJ ET BT 26.250 434.481 Td /F1 9.8 Tf [(assess this type of phenotype in rodents, with issues in consummatory vs. incentive motivation reflected in general alterations in )] TJ ET BT 26.250 422.576 Td /F1 9.8 Tf [(fixed- versus progressive alterations on an escalating-ratio schedules respectively, providing translational measures of the )] TJ ET BT 26.250 410.671 Td /F1 9.8 Tf [(amotivation/apathy construct of the human realm to the homologous construct of incentive motivation in preclinical models of )] TJ ET BT 26.250 398.766 Td /F1 9.8 Tf [(human disease.)] TJ ET BT 26.250 362.164 Td /F4 12.0 Tf [(Funding Statement)] TJ ET BT 26.250 342.210 Td /F1 9.8 Tf [(This work was supported by the CHDI Foundation. CHDI Foundation is a not-for-profit biomedical research organization )] TJ ET BT 26.250 330.305 Td /F1 9.8 Tf [(exclusively dedicated to discovering and developing therapeutics that slow the progression of Huntingtons disease. CHDI )] TJ ET BT 26.250 318.400 Td /F1 9.8 Tf [(Foundation conducts research in a number of different ways; for the purposes of this manuscript, all research was )] TJ ET BT 26.250 306.495 Td /F1 9.8 Tf [(conceptualized, planned, and directed by all authors listed and conducted at PsychoGenics, Inc., a contract research )] TJ ET BT 26.250 294.591 Td /F1 9.8 Tf [(organization.)] TJ ET BT 26.250 265.488 Td /F4 12.0 Tf [(Introduction)] TJ ET BT 26.250 245.534 Td /F1 9.8 Tf [(Huntingtons disease \(HD\) is an autosomal dominant genetic disorder, resulting from expansion of a stretch of CAG repeats )] TJ ET BT 26.250 233.629 Td /F1 9.8 Tf [(near the N-terminus of the Htt HD gene on chromosome 4, coding for the protein huntingtin. At present, HD is invariably fatal, )] TJ ET BT 26.250 221.724 Td /F1 9.8 Tf [(with the interval from the presentation of initial symptoms to death typically being around 15 to 20 years )] TJ ET 0.267 0.267 0.267 rg BT 473.873 223.232 Td /F4 8.7 Tf [(1)] TJ ET 0.271 0.267 0.267 rg BT 478.691 221.724 Td /F1 9.8 Tf [(. Clinically, HD is )] TJ ET BT 26.250 209.820 Td /F1 9.8 Tf [(characterized by a triad of symptom groups, with progressive dysfunction of motor, psychiatric and cognitive performance, with )] TJ ET BT 26.250 197.915 Td /F1 9.8 Tf [(the non-motor aspects of the disease generally held to cause a significant burden to patients and caregivers even relatively )] TJ ET BT 26.250 186.010 Td /F1 9.8 Tf [(early in disease progression )] TJ ET 0.267 0.267 0.267 rg BT 150.338 187.517 Td /F4 8.7 Tf [(2)] TJ ET 0.271 0.267 0.267 rg BT 155.157 186.010 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 166.605 Td /F1 9.8 Tf [(Apathy, frequently defined as a lack of motivation characterized by diminished goal-oriented behavior, is a major symptom in )] TJ ET BT 26.250 154.701 Td /F1 9.8 Tf [(HD, as well as other major neuropsychiatric disorders \(for review, see )] TJ ET 0.267 0.267 0.267 rg BT 329.124 156.208 Td /F4 8.7 Tf [(3)] TJ ET 0.271 0.267 0.267 rg BT 333.943 154.701 Td /F1 9.8 Tf [(\). In patients, motivational problems are seen once )] TJ ET BT 26.250 142.796 Td /F1 9.8 Tf [(disease state is relatively advanced, being observed in over half of moderately and severely affected patients )] TJ ET 0.267 0.267 0.267 rg BT 498.813 144.303 Td /F4 8.7 Tf [(4)] TJ ET 0.271 0.267 0.267 rg BT 503.632 142.796 Td /F1 9.8 Tf [(. Lack of )] TJ ET BT 26.250 130.891 Td /F1 9.8 Tf [(motivation is reflected in both lack of pleasure in normally rewarding activities and diminished behavior aimed at obtaining such )] TJ ET BT 26.250 118.986 Td /F1 9.8 Tf [(rewards. Although very prevalent in all neurodegenerative disorders and a major contributor to both the psychiatric pain of the )] TJ ET BT 26.250 107.082 Td /F1 9.8 Tf [(patient and the burden for the caregiver, apathy has rarely been the focus of in-depth research in the clinical domain, due to )] TJ ET BT 26.250 95.177 Td /F1 9.8 Tf [(lack of consensus about its definition and proper measurement with traditional scales like SANS, PANSS and UPDRS. Now that )] TJ ET BT 26.250 83.272 Td /F1 9.8 Tf [(specific and reliable scales, such as Marins apathy evaluation scale \(AES\), have been set up and validated )] TJ ET 0.267 0.267 0.267 rg BT 492.300 84.779 Td /F4 8.7 Tf [(5)] TJ ET BT 497.119 84.779 Td /F4 8.7 Tf [(6)] TJ ET BT 501.937 84.779 Td /F4 8.7 Tf [(7)] TJ ET 0.271 0.267 0.267 rg BT 506.756 83.272 Td /F1 9.8 Tf [(, the scene is )] TJ ET BT 26.250 71.367 Td /F1 9.8 Tf [(appropriately set for advancing the understanding of this symptom in both neurodegenerative and psychiatric disorders. )] TJ ET BT 26.250 59.463 Td /F1 9.8 Tf [(Consummatory \(direct pleasure derived from the reward, or hedonia\) and incentive-anticipatory components \(that drive reward- )] TJ ET BT 26.250 47.558 Td /F1 9.8 Tf [(or goal-directed behavior\) of motivation have been recognized both in the clinic and in the experimental analysis of behavior )] TJ ET 0.267 0.267 0.267 rg BT 562.207 49.065 Td /F4 8.7 Tf [(8)] TJ ET BT 567.026 49.065 Td /F4 8.7 Tf [(9)] TJ ET BT 26.250 37.160 Td /F4 8.7 Tf [(10)] TJ ET BT 35.887 37.160 Td /F4 8.7 Tf [(11)] TJ ET Q q 15.000 659.406 577.500 78.594 re W n 0.267 0.267 0.267 rg BT 15.000 718.042 Td /F2 21.0 Tf [(A mixed fixed ratio/progressive ratio procedure reveals an )] TJ ET BT 15.000 693.094 Td /F2 21.0 Tf [(apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 15.000 668.146 Td /F2 21.0 Tf [(models of Huntingtons disease)] TJ ET Q 0.271 0.267 0.267 rg BT 15.000 650.140 Td /F3 9.8 Tf [(April 25, 2012)] TJ ET BT 76.483 650.140 Td /F3 9.8 Tf [()] TJ ET 0.267 0.267 0.267 rg BT 81.358 650.140 Td /F3 9.8 Tf [(HD Models)] TJ ET BT 26.250 638.299 Td /F1 9.8 Tf [(Stephen Oakeshott)] TJ ET 0.271 0.267 0.267 rg BT 109.720 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 115.141 638.299 Td /F1 9.8 Tf [(Russell Port)] TJ ET 0.271 0.267 0.267 rg BT 167.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 173.114 638.299 Td /F1 9.8 Tf [(Jane Cummins-Sutphen)] TJ ET 0.271 0.267 0.267 rg BT 277.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 283.114 638.299 Td /F1 9.8 Tf [(Jason Berger)] TJ ET 0.271 0.267 0.267 rg BT 341.097 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 346.518 638.299 Td /F1 9.8 Tf [(Judy Watson-Johnson)] TJ ET 0.271 0.267 0.267 rg BT 442.975 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 448.396 638.299 Td /F1 9.8 Tf [(Sylvie Ramboz)] TJ ET 0.271 0.267 0.267 rg BT 513.409 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 518.830 638.299 Td /F1 9.8 Tf [(Neil Paterson)] TJ ET 0.271 0.267 0.267 rg BT 26.250 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 31.671 626.394 Td /F1 9.8 Tf [(Seung Kwak)] TJ ET 0.271 0.267 0.267 rg BT 86.407 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 91.829 626.394 Td /F1 9.8 Tf [(David Howland)] TJ ET 0.271 0.267 0.267 rg BT 157.387 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 162.808 626.394 Td /F1 9.8 Tf [(Dani Brunner)] TJ ET 0.271 0.267 0.267 rg BT 26.250 614.490 Td /F1 9.8 Tf [(Oakeshott S, Port R, Cummins-Sutphen J, Berger J, Watson-Johnson J, Ramboz S, Paterson N, Kwak S, Howland D, Brunner )] TJ ET BT 26.250 602.585 Td /F1 9.8 Tf [(D. A mixed fixed ratio/progressive ratio procedure reveals an apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 26.250 590.680 Td /F1 9.8 Tf [(models of Huntingtons disease. PLOS Currents Huntington Disease. 2012 Apr 25 . Edition 1. doi: 10.1371/4f972cffe82c0.)] TJ ET q 15.000 33.272 577.500 555.027 re W n 0.271 0.267 0.267 rg BT 26.250 561.578 Td /F4 12.0 Tf [(Abstract)] TJ ET BT 26.250 541.623 Td /F1 9.8 Tf [(Apathy, characterized by generally reduced interest in and likelihood to perform goal-directed actions, is a recognized symptom )] TJ ET BT 26.250 529.719 Td /F1 9.8 Tf [(of Huntingtons disease \(HD\), a devastating neurological disorder caused by a CAG repeat expansion of the Htt gene located on )] TJ ET BT 26.250 517.814 Td /F1 9.8 Tf [(chromosome 4. The present experiments used a modified progressive ratio task that incorporated a fixed-ratio schedule of )] TJ ET BT 26.250 505.909 Td /F1 9.8 Tf [(reinforcement component to assess consummatory behavior, and a progressive-ratio schedule component that required )] TJ ET BT 26.250 494.004 Td /F1 9.8 Tf [(increasing numbers of lever-presses for successive reinforcers \(0.01 ml of evaporated milk\). The studies revealed an apathetic )] TJ ET BT 26.250 482.100 Td /F1 9.8 Tf [(phenotype in two mouse models of HD, with decreased response rates either overall or only at higher ratio requirements in the )] TJ ET BT 26.250 470.195 Td /F1 9.8 Tf [(progressive-ratio component relative to wild-type controls. Based on the procedure used \(within-session fixed- and progressive-)] TJ ET BT 26.250 458.290 Td /F1 9.8 Tf [(ratio components\), it is proposed that an observed phenotype can be ascribed either specifically to reduced motivation to work )] TJ ET BT 26.250 446.385 Td /F1 9.8 Tf [(for food reinforcement or more generally to deficits in consummatory behavior. This procedure provides a simple means to )] TJ ET BT 26.250 434.481 Td /F1 9.8 Tf [(assess this type of phenotype in rodents, with issues in consummatory vs. incentive motivation reflected in general alterations in )] TJ ET BT 26.250 422.576 Td /F1 9.8 Tf [(fixed- versus progressive alterations on an escalating-ratio schedules respectively, providing translational measures of the )] TJ ET BT 26.250 410.671 Td /F1 9.8 Tf [(amotivation/apathy construct of the human realm to the homologous construct of incentive motivation in preclinical models of )] TJ ET BT 26.250 398.766 Td /F1 9.8 Tf [(human disease.)] TJ ET BT 26.250 362.164 Td /F4 12.0 Tf [(Funding Statement)] TJ ET BT 26.250 342.210 Td /F1 9.8 Tf [(This work was supported by the CHDI Foundation. CHDI Foundation is a not-for-profit biomedical research organization )] TJ ET BT 26.250 330.305 Td /F1 9.8 Tf [(exclusively dedicated to discovering and developing therapeutics that slow the progression of Huntingtons disease. CHDI )] TJ ET BT 26.250 318.400 Td /F1 9.8 Tf [(Foundation conducts research in a number of different ways; for the purposes of this manuscript, all research was )] TJ ET BT 26.250 306.495 Td /F1 9.8 Tf [(conceptualized, planned, and directed by all authors listed and conducted at PsychoGenics, Inc., a contract research )] TJ ET BT 26.250 294.591 Td /F1 9.8 Tf [(organization.)] TJ ET BT 26.250 265.488 Td /F4 12.0 Tf [(Introduction)] TJ ET BT 26.250 245.534 Td /F1 9.8 Tf [(Huntingtons disease \(HD\) is an autosomal dominant genetic disorder, resulting from expansion of a stretch of CAG repeats )] TJ ET BT 26.250 233.629 Td /F1 9.8 Tf [(near the N-terminus of the Htt HD gene on chromosome 4, coding for the protein huntingtin. At present, HD is invariably fatal, )] TJ ET BT 26.250 221.724 Td /F1 9.8 Tf [(with the interval from the presentation of initial symptoms to death typically being around 15 to 20 years )] TJ ET 0.267 0.267 0.267 rg BT 473.873 223.232 Td /F4 8.7 Tf [(1)] TJ ET 0.271 0.267 0.267 rg BT 478.691 221.724 Td /F1 9.8 Tf [(. Clinically, HD is )] TJ ET BT 26.250 209.820 Td /F1 9.8 Tf [(characterized by a triad of symptom groups, with progressive dysfunction of motor, psychiatric and cognitive performance, with )] TJ ET BT 26.250 197.915 Td /F1 9.8 Tf [(the non-motor aspects of the disease generally held to cause a significant burden to patients and caregivers even relatively )] TJ ET BT 26.250 186.010 Td /F1 9.8 Tf [(early in disease progression )] TJ ET 0.267 0.267 0.267 rg BT 150.338 187.517 Td /F4 8.7 Tf [(2)] TJ ET 0.271 0.267 0.267 rg BT 155.157 186.010 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 166.605 Td /F1 9.8 Tf [(Apathy, frequently defined as a lack of motivation characterized by diminished goal-oriented behavior, is a major symptom in )] TJ ET BT 26.250 154.701 Td /F1 9.8 Tf [(HD, as well as other major neuropsychiatric disorders \(for review, see )] TJ ET 0.267 0.267 0.267 rg BT 329.124 156.208 Td /F4 8.7 Tf [(3)] TJ ET 0.271 0.267 0.267 rg BT 333.943 154.701 Td /F1 9.8 Tf [(\). In patients, motivational problems are seen once )] TJ ET BT 26.250 142.796 Td /F1 9.8 Tf [(disease state is relatively advanced, being observed in over half of moderately and severely affected patients )] TJ ET 0.267 0.267 0.267 rg BT 498.813 144.303 Td /F4 8.7 Tf [(4)] TJ ET 0.271 0.267 0.267 rg BT 503.632 142.796 Td /F1 9.8 Tf [(. Lack of )] TJ ET BT 26.250 130.891 Td /F1 9.8 Tf [(motivation is reflected in both lack of pleasure in normally rewarding activities and diminished behavior aimed at obtaining such )] TJ ET BT 26.250 118.986 Td /F1 9.8 Tf [(rewards. Although very prevalent in all neurodegenerative disorders and a major contributor to both the psychiatric pain of the )] TJ ET BT 26.250 107.082 Td /F1 9.8 Tf [(patient and the burden for the caregiver, apathy has rarely been the focus of in-depth research in the clinical domain, due to )] TJ ET BT 26.250 95.177 Td /F1 9.8 Tf [(lack of consensus about its definition and proper measurement with traditional scales like SANS, PANSS and UPDRS. Now that )] TJ ET BT 26.250 83.272 Td /F1 9.8 Tf [(specific and reliable scales, such as Marins apathy evaluation scale \(AES\), have been set up and validated )] TJ ET 0.267 0.267 0.267 rg BT 492.300 84.779 Td /F4 8.7 Tf [(5)] TJ ET BT 497.119 84.779 Td /F4 8.7 Tf [(6)] TJ ET BT 501.937 84.779 Td /F4 8.7 Tf [(7)] TJ ET 0.271 0.267 0.267 rg BT 506.756 83.272 Td /F1 9.8 Tf [(, the scene is )] TJ ET BT 26.250 71.367 Td /F1 9.8 Tf [(appropriately set for advancing the understanding of this symptom in both neurodegenerative and psychiatric disorders. )] TJ ET BT 26.250 59.463 Td /F1 9.8 Tf [(Consummatory \(direct pleasure derived from the reward, or hedonia\) and incentive-anticipatory components \(that drive reward- )] TJ ET BT 26.250 47.558 Td /F1 9.8 Tf [(or goal-directed behavior\) of motivation have been recognized both in the clinic and in the experimental analysis of behavior )] TJ ET 0.267 0.267 0.267 rg BT 562.207 49.065 Td /F4 8.7 Tf [(8)] TJ ET BT 567.026 49.065 Td /F4 8.7 Tf [(9)] TJ ET BT 26.250 37.160 Td /F4 8.7 Tf [(10)] TJ ET BT 35.887 37.160 Td /F4 8.7 Tf [(11)] TJ ET Q q 15.000 659.406 577.500 78.594 re W n 0.267 0.267 0.267 rg BT 15.000 718.042 Td /F2 21.0 Tf [(A mixed fixed ratio/progressive ratio procedure reveals an )] TJ ET BT 15.000 693.094 Td /F2 21.0 Tf [(apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 15.000 668.146 Td /F2 21.0 Tf [(models of Huntingtons disease)] TJ ET Q 0.271 0.267 0.267 rg BT 15.000 650.140 Td /F3 9.8 Tf [(April 25, 2012)] TJ ET BT 76.483 650.140 Td /F3 9.8 Tf [()] TJ ET 0.267 0.267 0.267 rg BT 81.358 650.140 Td /F3 9.8 Tf [(HD Models)] TJ ET BT 26.250 638.299 Td /F1 9.8 Tf [(Stephen Oakeshott)] TJ ET 0.271 0.267 0.267 rg BT 109.720 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 115.141 638.299 Td /F1 9.8 Tf [(Russell Port)] TJ ET 0.271 0.267 0.267 rg BT 167.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 173.114 638.299 Td /F1 9.8 Tf [(Jane Cummins-Sutphen)] TJ ET 0.271 0.267 0.267 rg BT 277.693 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 283.114 638.299 Td /F1 9.8 Tf [(Jason Berger)] TJ ET 0.271 0.267 0.267 rg BT 341.097 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 346.518 638.299 Td /F1 9.8 Tf [(Judy Watson-Johnson)] TJ ET 0.271 0.267 0.267 rg BT 442.975 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 448.396 638.299 Td /F1 9.8 Tf [(Sylvie Ramboz)] TJ ET 0.271 0.267 0.267 rg BT 513.409 638.299 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 518.830 638.299 Td /F1 9.8 Tf [(Neil Paterson)] TJ ET 0.271 0.267 0.267 rg BT 26.250 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 31.671 626.394 Td /F1 9.8 Tf [(Seung Kwak)] TJ ET 0.271 0.267 0.267 rg BT 86.407 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 91.829 626.394 Td /F1 9.8 Tf [(David Howland)] TJ ET 0.271 0.267 0.267 rg BT 157.387 626.394 Td /F1 9.8 Tf [(, )] TJ ET 0.267 0.267 0.267 rg BT 162.808 626.394 Td /F1 9.8 Tf [(Dani Brunner)] TJ ET 0.271 0.267 0.267 rg BT 26.250 614.490 Td /F1 9.8 Tf [(Oakeshott S, Port R, Cummins-Sutphen J, Berger J, Watson-Johnson J, Ramboz S, Paterson N, Kwak S, Howland D, Brunner )] TJ ET BT 26.250 602.585 Td /F1 9.8 Tf [(D. A mixed fixed ratio/progressive ratio procedure reveals an apathy phenotype in the BAC HD and the z_Q175 KI mouse )] TJ ET BT 26.250 590.680 Td /F1 9.8 Tf [(models of Huntingtons disease. PLOS Currents Huntington Disease. 2012 Apr 25 . Edition 1. doi: 10.1371/4f972cffe82c0.)] TJ ET q 15.000 33.272 577.500 555.027 re W n 0.271 0.267 0.267 rg BT 26.250 561.578 Td /F4 12.0 Tf [(Abstract)] TJ ET BT 26.250 541.623 Td /F1 9.8 Tf [(Apathy, characterized by generally reduced interest in and likelihood to perform goal-directed actions, is a recognized symptom )] TJ ET BT 26.250 529.719 Td /F1 9.8 Tf [(of Huntingtons disease \(HD\), a devastating neurological disorder caused by a CAG repeat expansion of the Htt gene located on )] TJ ET BT 26.250 517.814 Td /F1 9.8 Tf [(chromosome 4. The present experiments used a modified progressive ratio task that incorporated a fixed-ratio schedule of )] TJ ET BT 26.250 505.909 Td /F1 9.8 Tf [(reinforcement component to assess consummatory behavior, and a progressive-ratio schedule component that required )] TJ ET BT 26.250 494.004 Td /F1 9.8 Tf [(increasing numbers of lever-presses for successive reinforcers \(0.01 ml of evaporated milk\). The studies revealed an apathetic )] TJ ET BT 26.250 482.100 Td /F1 9.8 Tf [(phenotype in two mouse models of HD, with decreased response rates either overall or only at higher ratio requirements in the )] TJ ET BT 26.250 470.195 Td /F1 9.8 Tf [(progressive-ratio component relative to wild-type controls. Based on the procedure used \(within-session fixed- and progressive-)] TJ ET BT 26.250 458.290 Td /F1 9.8 Tf [(ratio components\), it is proposed that an observed phenotype can be ascribed either specifically to reduced motivation to work )] TJ ET BT 26.250 446.385 Td /F1 9.8 Tf [(for food reinforcement or more generally to deficits in consummatory behavior. This procedure provides a simple means to )] TJ ET BT 26.250 434.481 Td /F1 9.8 Tf [(assess this type of phenotype in rodents, with issues in consummatory vs. incentive motivation reflected in general alterations in )] TJ ET BT 26.250 422.576 Td /F1 9.8 Tf [(fixed- versus progressive alterations on an escalating-ratio schedules respectively, providing translational measures of the )] TJ ET BT 26.250 410.671 Td /F1 9.8 Tf [(amotivation/apathy construct of the human realm to the homologous construct of incentive motivation in preclinical models of )] TJ ET BT 26.250 398.766 Td /F1 9.8 Tf [(human disease.)] TJ ET BT 26.250 362.164 Td /F4 12.0 Tf [(Funding Statement)] TJ ET BT 26.250 342.210 Td /F1 9.8 Tf [(This work was supported by the CHDI Foundation. CHDI Foundation is a not-for-profit biomedical research organization )] TJ ET BT 26.250 330.305 Td /F1 9.8 Tf [(exclusively dedicated to discovering and developing therapeutics that slow the progression of Huntingtons disease. CHDI )] TJ ET BT 26.250 318.400 Td /F1 9.8 Tf [(Foundation conducts research in a number of different ways; for the purposes of this manuscript, all research was )] TJ ET BT 26.250 306.495 Td /F1 9.8 Tf [(conceptualized, planned, and directed by all authors listed and conducted at PsychoGenics, Inc., a contract research )] TJ ET BT 26.250 294.591 Td /F1 9.8 Tf [(organization.)] TJ ET BT 26.250 265.488 Td /F4 12.0 Tf [(Introduction)] TJ ET BT 26.250 245.534 Td /F1 9.8 Tf [(Huntingtons disease \(HD\) is an autosomal dominant genetic disorder, resulting from expansion of a stretch of CAG repeats )] TJ ET BT 26.250 233.629 Td /F1 9.8 Tf [(near the N-terminus of the Htt HD gene on chromosome 4, coding for the protein huntingtin. At present, HD is invariably fatal, )] TJ ET BT 26.250 221.724 Td /F1 9.8 Tf [(with the interval from the presentation of initial symptoms to death typically being around 15 to 20 years )] TJ ET 0.267 0.267 0.267 rg BT 473.873 223.232 Td /F4 8.7 Tf [(1)] TJ ET 0.271 0.267 0.267 rg BT 478.691 221.724 Td /F1 9.8 Tf [(. Clinically, HD is )] TJ ET BT 26.250 209.820 Td /F1 9.8 Tf [(characterized by a triad of symptom groups, with progressive dysfunction of motor, psychiatric and cognitive performance, with )] TJ ET BT 26.250 197.915 Td /F1 9.8 Tf [(the non-motor aspects of the disease generally held to cause a significant burden to patients and caregivers even relatively )] TJ ET BT 26.250 186.010 Td /F1 9.8 Tf [(early in disease progression )] TJ ET 0.267 0.267 0.267 rg BT 150.338 187.517 Td /F4 8.7 Tf [(2)] TJ ET 0.271 0.267 0.267 rg BT 155.157 186.010 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 166.605 Td /F1 9.8 Tf [(Apathy, frequently defined as a lack of motivation characterized by diminished goal-oriented behavior, is a major symptom in )] TJ ET BT 26.250 154.701 Td /F1 9.8 Tf [(HD, as well as other major neuropsychiatric disorders \(for review, see )] TJ ET 0.267 0.267 0.267 rg BT 329.124 156.208 Td /F4 8.7 Tf [(3)] TJ ET 0.271 0.267 0.267 rg BT 333.943 154.701 Td /F1 9.8 Tf [(\). In patients, motivational problems are seen once )] TJ ET BT 26.250 142.796 Td /F1 9.8 Tf [(disease state is relatively advanced, being observed in over half of moderately and severely affected patients )] TJ ET 0.267 0.267 0.267 rg BT 498.813 144.303 Td /F4 8.7 Tf [(4)] TJ ET 0.271 0.267 0.267 rg BT 503.632 142.796 Td /F1 9.8 Tf [(. Lack of )] TJ ET BT 26.250 130.891 Td /F1 9.8 Tf [(motivation is reflected in both lack of pleasure in normally rewarding activities and diminished behavior aimed at obtaining such )] TJ ET BT 26.250 118.986 Td /F1 9.8 Tf [(rewards. Although very prevalent in all neurodegenerative disorders and a major contributor to both the psychiatric pain of the )] TJ ET BT 26.250 107.082 Td /F1 9.8 Tf [(patient and the burden for the caregiver, apathy has rarely been the focus of in-depth research in the clinical domain, due to )] TJ ET BT 26.250 95.177 Td /F1 9.8 Tf [(lack of consensus about its definition and proper measurement with traditional scales like SANS, PANSS and UPDRS. Now that )] TJ ET BT 26.250 83.272 Td /F1 9.8 Tf [(specific and reliable scales, such as Marins apathy evaluation scale \(AES\), have been set up and validated )] TJ ET 0.267 0.267 0.267 rg BT 492.300 84.779 Td /F4 8.7 Tf [(5)] TJ ET BT 497.119 84.779 Td /F4 8.7 Tf [(6)] TJ ET BT 501.937 84.779 Td /F4 8.7 Tf [(7)] TJ ET 0.271 0.267 0.267 rg BT 506.756 83.272 Td /F1 9.8 Tf [(, the scene is )] TJ ET BT 26.250 71.367 Td /F1 9.8 Tf [(appropriately set for advancing the understanding of this symptom in both neurodegenerative and psychiatric disorders. )] TJ ET BT 26.250 59.463 Td /F1 9.8 Tf [(Consummatory \(direct pleasure derived from the reward, or hedonia\) and incentive-anticipatory components \(that drive reward- )] TJ ET BT 26.250 47.558 Td /F1 9.8 Tf [(or goal-directed behavior\) of motivation have been recognized both in the clinic and in the experimental analysis of behavior )] TJ ET 0.267 0.267 0.267 rg BT 562.207 49.065 Td /F4 8.7 Tf [(8)] TJ ET BT 567.026 49.065 Td /F4 8.7 Tf [(9)] TJ ET BT 26.250 37.160 Td /F4 8.7 Tf [(10)] TJ ET BT 35.887 37.160 Td /F4 8.7 Tf [(11)] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(1)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 8 0 obj << /Type /Font /Subtype /Type1 /Name /F1 /BaseFont /Helvetica /Encoding /WinAnsiEncoding >> endobj 9 0 obj << /Type /Font /Subtype /Type1 /Name /F2 /BaseFont /Times-Bold /Encoding /WinAnsiEncoding >> endobj 10 0 obj << /Type /Font /Subtype /Type1 /Name /F3 /BaseFont /Times-Italic /Encoding /WinAnsiEncoding >> endobj 11 0 obj << /Type /Font /Subtype /Type1 /Name /F4 /BaseFont /Helvetica-Bold /Encoding /WinAnsiEncoding >> endobj 12 0 obj << /Type /XObject /Subtype /Image /Width 500 /Height 52 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 500 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 144>> stream x1 0 'ݲ؎"e{dzAdzAdzAdzAdzAdzAdzAdzAdzAdzAdzAdzAtlM0\ endstream endobj 13 0 obj << /Type /XObject /Subtype /Image /Width 500 /Height 52 /SMask 12 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 500 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 4223>> stream xە8=,8C#h!hGv#0=j$q1uaNĥT*J&a_Zaa0 a0 a0 H^UUUUX!0 3SY|^G0 ÌÔ{]wa_>"OeYaG!8e}a6̖{4M&d:"qʲ\QUUMS<ϛNld2l6Z a<=2B(2vK7M\.GW0 rrWWavߖe9C?Eq8Gqy~~}_qdϽ(neYʒGjK4EQNjͷm{X?|7m۶mqP:Ȳp8Kmu]lNzuZ,NTeY"jlUUQW\,aRiVbȗLkHkEsjd.L6=Iו P]1t7Sc2uIuMM; K5>A$Ǒ$I׋nY 0_Hat;aq䃰U$nR ,zO7bT mhhԫܻu1F*qm'P}ܙ<ϕALO6Lw3nB.T4co{߽.lV $laԀIu!gwChxJ]Ne (+M"d-Eqc=P ! "nf(7>ce,Řm[!ʝD!m)Bs@)ta3}g˲}ZbշONKFp倫Rg(,I2 C%5y B v o9϶m+ІXcTwV5;8dHF{7Qm6'N۠%$HSMk!D4i:ŕS*bS̹"2=ԓH51$UfZVtli<0]nDZlcWv;LQrxhk+},CVeD8]ې/.m[nc)2XAOMo&}"&ڨv宇EqX$tcGc,|`5)yO(U\͠&ԭC&~1ynKoC~kqqltV\4M8NTre#sT|>+GJ W]$Ii EMHdD70_ rEЀa:c4u U0.RH=SN4/z2pȤfx$]1 wt<]׭U$%=wi~ܮ{=×w\Բ!s^__=Axa1|=(l´i4MEUUm6y)~E5UU~gѥJ'+WTN'!H>{=nytH{{xzo<8aOw0lN]? gNR2Ɍ6Z摤Ww(eYv̲ b\aHɟ,J6 ܺ= ^'ҵ4^.˲rIaJ'RC]Qѯr洱lf?>ȩ_Ye=3ʆ:Fy0D&y%0zU^5ἧԯgFeYqƈhz#ms[o$+0Qz2-Z\"#f29:WGVzlBPD;CW+Dye$ 6HQ"\&_$Tp]Ah eHyR1' p'sD)ZDpB/Y_SVGӌ;Oo[Am+(Uܮ+UʽN=PJ/>^;?9ogmYѲ=k@._ (V{#z/,@Y,1e`& i$<=EQl6|A4b%'X[ѶaSh/QM&$NgSٲ,奭ֻDH>EeSFo['ķriVa߶ ї5,lj* QÈ=TqEMD 4N6MCL(PƯ%n^ w~oSrDfe0^F]G$IY?v+rEQ-WUmpP^ :vz֝2q#3Dƾt: ~Gv(,$j:NpCˏS)=ϛL&xlۆ+i8}CH,t%z\MD 0 ^tC>(4;ن]螞Pt:5f[@oqg{L̷gYD"maI4coKE殂 'ٲt2_,m[|QafLuDZ( W 5oOiy{0oqNF;rj%zwuo10)BwܮC!}Έ3H(.E' h]jDA 6.M T8zqy~oeO#]PWp0<;F܆b+ɽnKQy\8nڶd/خ^^o|N.[vOz`'Ef̈+""/cL<" Ƅ_~W|¿Tt"fiY_uWx<ŠM(0L891a>ao\|[C~mX3 3;o6|>ϻ#ChR0 Ì`r뚢#~#N*0 Fk%ʪrOd6u͊Akdfwܧ),Y0 ܡܛ4ۈa 0 -0 )rgrgrgrgzm endstream endobj 14 0 obj << /Type /Annot /Subtype /Link /A 15 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 716.1516 543.4230 736.9416 ] >> endobj 15 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 16 0 obj << /Type /Annot /Subtype /Link /A 17 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 691.2036 560.4540 711.9936 ] >> endobj 17 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 18 0 obj << /Type /Annot /Subtype /Link /A 19 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 666.2556 293.2710 687.0456 ] >> endobj 19 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 20 0 obj << /Type /Annot /Subtype /Link /A 21 0 R /Border [0 0 0] /H /I /Rect [ 81.3585 649.2621 126.5790 658.9146 ] >> endobj 21 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article_category/hd-models/) >> endobj 22 0 obj << /Type /Annot /Subtype /Link /A 23 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 637.3973 109.7198 647.3179 ] >> endobj 23 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/stephenoakeshott/) >> endobj 24 0 obj << /Type /Annot /Subtype /Link /A 25 0 R /Border [0 0 0] /H /I /Rect [ 115.1408 637.3973 167.6933 647.3179 ] >> endobj 25 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/russellport/) >> endobj 26 0 obj << /Type /Annot /Subtype /Link /A 27 0 R /Border [0 0 0] /H /I /Rect [ 173.1142 637.3973 277.6927 647.3179 ] >> endobj 27 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/janecumminssutphen/) >> endobj 28 0 obj << /Type /Annot /Subtype /Link /A 29 0 R /Border [0 0 0] /H /I /Rect [ 283.1137 637.3973 341.0970 647.3179 ] >> endobj 29 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/jasonberger/) >> endobj 30 0 obj << /Type /Annot /Subtype /Link /A 31 0 R /Border [0 0 0] /H /I /Rect [ 346.5180 637.3973 442.9747 647.3179 ] >> endobj 31 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/judywatsonjohnson/) >> endobj 32 0 obj << /Type /Annot /Subtype /Link /A 33 0 R /Border [0 0 0] /H /I /Rect [ 448.3957 637.3973 513.4087 647.3179 ] >> endobj 33 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/sylvieramboz/) >> endobj 34 0 obj << /Type /Annot /Subtype /Link /A 35 0 R /Border [0 0 0] /H /I /Rect [ 518.8297 637.3973 577.3492 647.3179 ] >> endobj 35 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/neilpaterson/) >> endobj 36 0 obj << /Type /Annot /Subtype /Link /A 37 0 R /Border [0 0 0] /H /I /Rect [ 31.6710 625.4926 86.4075 635.4132 ] >> endobj 37 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/seungkwak/) >> endobj 38 0 obj << /Type /Annot /Subtype /Link /A 39 0 R /Border [0 0 0] /H /I /Rect [ 91.8285 625.4926 157.3875 635.4132 ] >> endobj 39 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/davidhowland/) >> endobj 40 0 obj << /Type /Annot /Subtype /Link /A 41 0 R /Border [0 0 0] /H /I /Rect [ 162.8085 625.4926 220.2457 635.4132 ] >> endobj 41 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/danibrunner/) >> endobj 42 0 obj << /Type /Annot /Subtype /Link /A 43 0 R /Border [0 0 0] /H /I /Rect [ 473.8725 222.4299 478.6912 231.2483 ] >> endobj 43 0 obj << /Type /Action >> endobj 44 0 obj << /Type /Annot /Subtype /Link /A 45 0 R /Border [0 0 0] /H /I /Rect [ 150.3383 186.7157 155.1569 195.5340 ] >> endobj 45 0 obj << /Type /Action >> endobj 46 0 obj << /Type /Annot /Subtype /Link /A 47 0 R /Border [0 0 0] /H /I /Rect [ 329.1240 155.4062 333.9427 164.2245 ] >> endobj 47 0 obj << /Type /Action >> endobj 48 0 obj << /Type /Annot /Subtype /Link /A 49 0 R /Border [0 0 0] /H /I /Rect [ 498.8130 143.5014 503.6317 152.3197 ] >> endobj 49 0 obj << /Type /Action >> endobj 50 0 obj << /Type /Annot /Subtype /Link /A 51 0 R /Border [0 0 0] /H /I /Rect [ 492.3000 83.9777 497.1187 92.7960 ] >> endobj 51 0 obj << /Type /Action >> endobj 52 0 obj << /Type /Annot /Subtype /Link /A 53 0 R /Border [0 0 0] /H /I /Rect [ 497.1187 83.9777 501.9373 92.7960 ] >> endobj 53 0 obj << /Type /Action >> endobj 54 0 obj << /Type /Annot /Subtype /Link /A 55 0 R /Border [0 0 0] /H /I /Rect [ 501.9373 83.9777 506.7560 92.7960 ] >> endobj 55 0 obj << /Type /Action >> endobj 56 0 obj << /Type /Annot /Subtype /Link /A 57 0 R /Border [0 0 0] /H /I /Rect [ 562.2075 48.2634 567.0262 57.0817 ] >> endobj 57 0 obj << /Type /Action >> endobj 58 0 obj << /Type /Annot /Subtype /Link /A 59 0 R /Border [0 0 0] /H /I /Rect [ 567.0262 48.2634 571.8448 57.0817 ] >> endobj 59 0 obj << /Type /Action >> endobj 60 0 obj << /Type /Annot /Subtype /Link /A 61 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 36.3587 35.8873 45.1770 ] >> endobj 61 0 obj << /Type /Action >> endobj 62 0 obj << /Type /Annot /Subtype /Link /A 63 0 R /Border [0 0 0] /H /I /Rect [ 35.8873 36.3587 45.5247 45.1770 ] >> endobj 63 0 obj << /Type /Action >> endobj 64 0 obj << /Type /Annot /Subtype /Link /A 65 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 716.1516 543.4230 736.9416 ] >> endobj 65 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 66 0 obj << /Type /Annot /Subtype /Link /A 67 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 691.2036 560.4540 711.9936 ] >> endobj 67 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 68 0 obj << /Type /Annot /Subtype /Link /A 69 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 666.2556 293.2710 687.0456 ] >> endobj 69 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 70 0 obj << /Type /Annot /Subtype /Link /A 71 0 R /Border [0 0 0] /H /I /Rect [ 81.3585 649.2621 126.5790 658.9146 ] >> endobj 71 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article_category/hd-models/) >> endobj 72 0 obj << /Type /Annot /Subtype /Link /A 73 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 637.3973 109.7198 647.3179 ] >> endobj 73 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/stephenoakeshott/) >> endobj 74 0 obj << /Type /Annot /Subtype /Link /A 75 0 R /Border [0 0 0] /H /I /Rect [ 115.1408 637.3973 167.6933 647.3179 ] >> endobj 75 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/russellport/) >> endobj 76 0 obj << /Type /Annot /Subtype /Link /A 77 0 R /Border [0 0 0] /H /I /Rect [ 173.1142 637.3973 277.6927 647.3179 ] >> endobj 77 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/janecumminssutphen/) >> endobj 78 0 obj << /Type /Annot /Subtype /Link /A 79 0 R /Border [0 0 0] /H /I /Rect [ 283.1137 637.3973 341.0970 647.3179 ] >> endobj 79 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/jasonberger/) >> endobj 80 0 obj << /Type /Annot /Subtype /Link /A 81 0 R /Border [0 0 0] /H /I /Rect [ 346.5180 637.3973 442.9747 647.3179 ] >> endobj 81 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/judywatsonjohnson/) >> endobj 82 0 obj << /Type /Annot /Subtype /Link /A 83 0 R /Border [0 0 0] /H /I /Rect [ 448.3957 637.3973 513.4087 647.3179 ] >> endobj 83 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/sylvieramboz/) >> endobj 84 0 obj << /Type /Annot /Subtype /Link /A 85 0 R /Border [0 0 0] /H /I /Rect [ 518.8297 637.3973 577.3492 647.3179 ] >> endobj 85 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/neilpaterson/) >> endobj 86 0 obj << /Type /Annot /Subtype /Link /A 87 0 R /Border [0 0 0] /H /I /Rect [ 31.6710 625.4926 86.4075 635.4132 ] >> endobj 87 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/seungkwak/) >> endobj 88 0 obj << /Type /Annot /Subtype /Link /A 89 0 R /Border [0 0 0] /H /I /Rect [ 91.8285 625.4926 157.3875 635.4132 ] >> endobj 89 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/davidhowland/) >> endobj 90 0 obj << /Type /Annot /Subtype /Link /A 91 0 R /Border [0 0 0] /H /I /Rect [ 162.8085 625.4926 220.2457 635.4132 ] >> endobj 91 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/danibrunner/) >> endobj 92 0 obj << /Type /Annot /Subtype /Link /A 93 0 R /Border [0 0 0] /H /I /Rect [ 473.8725 222.4299 478.6912 231.2483 ] >> endobj 93 0 obj << /Type /Action >> endobj 94 0 obj << /Type /Annot /Subtype /Link /A 95 0 R /Border [0 0 0] /H /I /Rect [ 150.3383 186.7157 155.1569 195.5340 ] >> endobj 95 0 obj << /Type /Action >> endobj 96 0 obj << /Type /Annot /Subtype /Link /A 97 0 R /Border [0 0 0] /H /I /Rect [ 329.1240 155.4062 333.9427 164.2245 ] >> endobj 97 0 obj << /Type /Action >> endobj 98 0 obj << /Type /Annot /Subtype /Link /A 99 0 R /Border [0 0 0] /H /I /Rect [ 498.8130 143.5014 503.6317 152.3197 ] >> endobj 99 0 obj << /Type /Action >> endobj 100 0 obj << /Type /Annot /Subtype /Link /A 101 0 R /Border [0 0 0] /H /I /Rect [ 492.3000 83.9777 497.1187 92.7960 ] >> endobj 101 0 obj << /Type /Action >> endobj 102 0 obj << /Type /Annot /Subtype /Link /A 103 0 R /Border [0 0 0] /H /I /Rect [ 497.1187 83.9777 501.9373 92.7960 ] >> endobj 103 0 obj << /Type /Action >> endobj 104 0 obj << /Type /Annot /Subtype /Link /A 105 0 R /Border [0 0 0] /H /I /Rect [ 501.9373 83.9777 506.7560 92.7960 ] >> endobj 105 0 obj << /Type /Action >> endobj 106 0 obj << /Type /Annot /Subtype /Link /A 107 0 R /Border [0 0 0] /H /I /Rect [ 562.2075 48.2634 567.0262 57.0817 ] >> endobj 107 0 obj << /Type /Action >> endobj 108 0 obj << /Type /Annot /Subtype /Link /A 109 0 R /Border [0 0 0] /H /I /Rect [ 567.0262 48.2634 571.8448 57.0817 ] >> endobj 109 0 obj << /Type /Action >> endobj 110 0 obj << /Type /Annot /Subtype /Link /A 111 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 36.3587 35.8873 45.1770 ] >> endobj 111 0 obj << /Type /Action >> endobj 112 0 obj << /Type /Annot /Subtype /Link /A 113 0 R /Border [0 0 0] /H /I /Rect [ 35.8873 36.3587 45.5247 45.1770 ] >> endobj 113 0 obj << /Type /Action >> endobj 114 0 obj << /Type /Annot /Subtype /Link /A 115 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 716.1516 543.4230 736.9416 ] >> endobj 115 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 116 0 obj << /Type /Annot /Subtype /Link /A 117 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 691.2036 560.4540 711.9936 ] >> endobj 117 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 118 0 obj << /Type /Annot /Subtype /Link /A 119 0 R /Border [0 0 0] /H /I /Rect [ 15.0000 666.2556 293.2710 687.0456 ] >> endobj 119 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article/a-mixed-fixed-ratioprogressive-ratio-procedure-reveals-an-apathy-phenotype-in-the-bac-hd-and-the-z_q175-ki-mouse-models-of-huntingtons-disease/) >> endobj 120 0 obj << /Type /Annot /Subtype /Link /A 121 0 R /Border [0 0 0] /H /I /Rect [ 81.3585 649.2621 126.5790 658.9146 ] >> endobj 121 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/article_category/hd-models/) >> endobj 122 0 obj << /Type /Annot /Subtype /Link /A 123 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 637.3973 109.7198 647.3179 ] >> endobj 123 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/stephenoakeshott/) >> endobj 124 0 obj << /Type /Annot /Subtype /Link /A 125 0 R /Border [0 0 0] /H /I /Rect [ 115.1408 637.3973 167.6933 647.3179 ] >> endobj 125 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/russellport/) >> endobj 126 0 obj << /Type /Annot /Subtype /Link /A 127 0 R /Border [0 0 0] /H /I /Rect [ 173.1142 637.3973 277.6927 647.3179 ] >> endobj 127 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/janecumminssutphen/) >> endobj 128 0 obj << /Type /Annot /Subtype /Link /A 129 0 R /Border [0 0 0] /H /I /Rect [ 283.1137 637.3973 341.0970 647.3179 ] >> endobj 129 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/jasonberger/) >> endobj 130 0 obj << /Type /Annot /Subtype /Link /A 131 0 R /Border [0 0 0] /H /I /Rect [ 346.5180 637.3973 442.9747 647.3179 ] >> endobj 131 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/judywatsonjohnson/) >> endobj 132 0 obj << /Type /Annot /Subtype /Link /A 133 0 R /Border [0 0 0] /H /I /Rect [ 448.3957 637.3973 513.4087 647.3179 ] >> endobj 133 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/sylvieramboz/) >> endobj 134 0 obj << /Type /Annot /Subtype /Link /A 135 0 R /Border [0 0 0] /H /I /Rect [ 518.8297 637.3973 577.3492 647.3179 ] >> endobj 135 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/neilpaterson/) >> endobj 136 0 obj << /Type /Annot /Subtype /Link /A 137 0 R /Border [0 0 0] /H /I /Rect [ 31.6710 625.4926 86.4075 635.4132 ] >> endobj 137 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/seungkwak/) >> endobj 138 0 obj << /Type /Annot /Subtype /Link /A 139 0 R /Border [0 0 0] /H /I /Rect [ 91.8285 625.4926 157.3875 635.4132 ] >> endobj 139 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/davidhowland/) >> endobj 140 0 obj << /Type /Annot /Subtype /Link /A 141 0 R /Border [0 0 0] /H /I /Rect [ 162.8085 625.4926 220.2457 635.4132 ] >> endobj 141 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/author/danibrunner/) >> endobj 142 0 obj << /Type /Annot /Subtype /Link /A 143 0 R /Border [0 0 0] /H /I /Rect [ 473.8725 222.4299 478.6912 231.2483 ] >> endobj 143 0 obj << /Type /Action >> endobj 144 0 obj << /Type /Annot /Subtype /Link /A 145 0 R /Border [0 0 0] /H /I /Rect [ 150.3383 186.7157 155.1569 195.5340 ] >> endobj 145 0 obj << /Type /Action >> endobj 146 0 obj << /Type /Annot /Subtype /Link /A 147 0 R /Border [0 0 0] /H /I /Rect [ 329.1240 155.4062 333.9427 164.2245 ] >> endobj 147 0 obj << /Type /Action >> endobj 148 0 obj << /Type /Annot /Subtype /Link /A 149 0 R /Border [0 0 0] /H /I /Rect [ 498.8130 143.5014 503.6317 152.3197 ] >> endobj 149 0 obj << /Type /Action >> endobj 150 0 obj << /Type /Annot /Subtype /Link /A 151 0 R /Border [0 0 0] /H /I /Rect [ 492.3000 83.9777 497.1187 92.7960 ] >> endobj 151 0 obj << /Type /Action >> endobj 152 0 obj << /Type /Annot /Subtype /Link /A 153 0 R /Border [0 0 0] /H /I /Rect [ 497.1187 83.9777 501.9373 92.7960 ] >> endobj 153 0 obj << /Type /Action >> endobj 154 0 obj << /Type /Annot /Subtype /Link /A 155 0 R /Border [0 0 0] /H /I /Rect [ 501.9373 83.9777 506.7560 92.7960 ] >> endobj 155 0 obj << /Type /Action >> endobj 156 0 obj << /Type /Annot /Subtype /Link /A 157 0 R /Border [0 0 0] /H /I /Rect [ 562.2075 48.2634 567.0262 57.0817 ] >> endobj 157 0 obj << /Type /Action >> endobj 158 0 obj << /Type /Annot /Subtype /Link /A 159 0 R /Border [0 0 0] /H /I /Rect [ 567.0262 48.2634 571.8448 57.0817 ] >> endobj 159 0 obj << /Type /Action >> endobj 160 0 obj << /Type /Annot /Subtype /Link /A 161 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 36.3587 35.8873 45.1770 ] >> endobj 161 0 obj << /Type /Action >> endobj 162 0 obj << /Type /Annot /Subtype /Link /A 163 0 R /Border [0 0 0] /H /I /Rect [ 35.8873 36.3587 45.5247 45.1770 ] >> endobj 163 0 obj << /Type /Action >> endobj 164 0 obj << /Type /Page /Parent 3 0 R /Annots [ 167 0 R 169 0 R 171 0 R 173 0 R 175 0 R 177 0 R 179 0 R 181 0 R 183 0 R 185 0 R 187 0 R 189 0 R 191 0 R 193 0 R 195 0 R 197 0 R 199 0 R 201 0 R 203 0 R 205 0 R 207 0 R 209 0 R 211 0 R 213 0 R 215 0 R 217 0 R 219 0 R 221 0 R 223 0 R 225 0 R 227 0 R 229 0 R 231 0 R 233 0 R 235 0 R 237 0 R 239 0 R 241 0 R 243 0 R 245 0 R 247 0 R 249 0 R ] /Contents 165 0 R >> endobj 165 0 obj << /Length 27265 >> stream 0.267 0.267 0.267 rg q 15.000 23.301 577.500 753.699 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(Historically, researchers have utilized simple fixed-ratio schedules of reinforcement \(unchanging response requirement, typically )] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(at a low effort level\) or even simpler dose-response preference of rewards such as sucrose to measure consummatory )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(behaviors )] TJ ET 0.267 0.267 0.267 rg BT 71.227 725.769 Td /F4 8.7 Tf [(12)] TJ ET 0.271 0.267 0.267 rg BT 80.864 724.262 Td /F1 9.8 Tf [(. By contrast, progressive-ratio schedules \(increasing number of responses are required to obtain consecutive )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(deliveries of the reinforcer )] TJ ET 0.267 0.267 0.267 rg BT 141.125 713.864 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 150.762 712.357 Td /F1 9.8 Tf [(\), have been used to provide a more reliable and unambiguous measure of incentive motivation )] TJ ET 0.267 0.267 0.267 rg BT 564.250 713.864 Td /F4 8.7 Tf [(12)] TJ ET BT 26.250 701.960 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 35.887 700.452 Td /F1 9.8 Tf [(, in addition to providing a measure of reinforcer magnitude/efficacy. Typically, the final response requirement completed in )] TJ ET BT 26.250 688.548 Td /F1 9.8 Tf [(the test session is referred to as the break-point and has been used as the single performance measure. Recently however, )] TJ ET BT 26.250 676.643 Td /F1 9.8 Tf [(some have proposed the use of response rates at specific response ratios as a useful and informative output measure )] TJ ET 0.267 0.267 0.267 rg BT 537.832 678.150 Td /F4 8.7 Tf [(14)] TJ ET 0.271 0.267 0.267 rg BT 26.250 664.738 Td /F1 9.8 Tf [(although there is a risk of potential confounds with motor deficits if appropriate controls are not included in the experimental )] TJ ET BT 26.250 652.833 Td /F1 9.8 Tf [(design. Satiation is a potential confounding factor in this type of study, although the problem is much reduced under PR versus )] TJ ET BT 26.250 640.929 Td /F1 9.8 Tf [(FR schedules since delivery of reinforcers is typically far more limited under PR because of increasing response requirements.)] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(The motivation for the present experiments is to explore operant schedules of reinforcement to define an homologous )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(behavioral endpoint of the apathy symptom in the mouse, which, coupled with the relatively large number of preclinical HD )] TJ ET BT 26.250 597.714 Td /F1 9.8 Tf [(models now available )] TJ ET 0.267 0.267 0.267 rg BT 122.161 599.222 Td /F4 8.7 Tf [(15)] TJ ET BT 131.798 599.222 Td /F4 8.7 Tf [(16)] TJ ET BT 141.435 599.222 Td /F4 8.7 Tf [(17)] TJ ET BT 151.073 599.222 Td /F4 8.7 Tf [(18)] TJ ET BT 160.710 599.222 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 170.347 597.714 Td /F1 9.8 Tf [(, would be of great value for screening of possible therapeutics. Therefore, a mixed low effort )] TJ ET BT 26.250 585.810 Td /F1 9.8 Tf [(fixed- and escalating- effort progressive-ratio \(FR and PR, respectively\) procedure was utilized to explore consummatory )] TJ ET BT 26.250 573.905 Td /F1 9.8 Tf [(behavior \(relevant to immediate rewarding or reinforcing properties of a stimulus with the FR component\), and incentive )] TJ ET BT 26.250 562.000 Td /F1 9.8 Tf [(motivation \(PR component\). Two mouse models of HD were characterized in the task; specifically, the BAC HD full-length )] TJ ET BT 26.250 550.095 Td /F1 9.8 Tf [(transgenic model generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 222.410 551.603 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 232.048 550.095 Td /F1 9.8 Tf [(, and the z_Q175 knock-in model generated at PsychoGenics Inc. )] TJ ET 0.267 0.267 0.267 rg BT 518.737 551.603 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 528.374 550.095 Td /F1 9.8 Tf [(. As far as )] TJ ET BT 26.250 538.191 Td /F1 9.8 Tf [(these authors are aware, neither mouse has been shown to exhibit an apathetic phenotype. It was predicted that an HD mouse )] TJ ET BT 26.250 526.286 Td /F1 9.8 Tf [(model with an apathetic phenotype will reduce its work rate earlier in the test session, at a lower ratio requirement, than will a )] TJ ET BT 26.250 514.381 Td /F1 9.8 Tf [(healthy WT.)] TJ ET BT 26.250 477.779 Td /F4 12.0 Tf [(Experiment 1A)] TJ ET BT 26.250 457.824 Td /F1 9.8 Tf [(The initial experiments were conducted using adult BAC HD mice, a full-length transgenic model developed in the laboratory of )] TJ ET BT 26.250 445.920 Td /F1 9.8 Tf [(X. William Yang )] TJ ET 0.267 0.267 0.267 rg BT 97.766 447.427 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 26.250 426.515 Td /F5 9.8 Tf [(Materials and Methods)] TJ ET BT 26.250 407.110 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 387.705 Td /F1 9.8 Tf [(WT and transgenic BAC mice \(BAC HD, CHDI-007-\(3\)\(1\)\) were generated crossing dams carrying ~97 stable CAGCAA repeats )] TJ ET BT 26.250 375.801 Td /F1 9.8 Tf [(on a C57Bl/6J with FVB/NJ male mice at the Jackson Laboratory \(Bar Harbor, ME\) and shipped to the test facility as adults. )] TJ ET BT 26.250 363.896 Td /F1 9.8 Tf [(Mice were maintained in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to water.)] TJ ET BT 26.250 344.491 Td /F1 9.8 Tf [(Once acclimated to our colony, the animals were pair-housed and food restricted, with WT mice reduced to 85% of their free-)] TJ ET BT 26.250 332.586 Td /F1 9.8 Tf [(feeding body weights. The BAC Tg mice, which develop excess fat deposits, were food-restricted gradually to the point where a )] TJ ET BT 26.250 320.682 Td /F1 9.8 Tf [(matched control group consumed comparable amounts of food to 85% WT animals in a 30-min free feeding test, at which point )] TJ ET BT 26.250 308.777 Td /F1 9.8 Tf [(they were slightly heavier than controls \(mean 36.5 g for BAC mice, 28.1 g for WT controls on the initial day of training\). Mice )] TJ ET BT 26.250 296.872 Td /F1 9.8 Tf [(were maintained at their new target weights by daily provision of limited quantities of food \(BIO-SERV 500mg pellets\).)] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(A single group of female mice was evaluated, 8 animals per genotype. The mice had previously been evaluated in the )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(PhenoCube \(described in )] TJ ET 0.267 0.267 0.267 rg BT 138.970 267.070 Td /F4 8.7 Tf [(20)] TJ ET 0.271 0.267 0.267 rg BT 148.607 265.563 Td /F1 9.8 Tf [(\), an automated phenotyping system, but were nave to operant boxes and had never before been )] TJ ET BT 26.250 253.658 Td /F1 9.8 Tf [(food restricted. All mice were 73 weeks old 1 week on the initial day of instrumental training and were 79 weeks old 1 week )] TJ ET BT 26.250 241.753 Td /F1 9.8 Tf [(on the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 222.348 Td /F1 9.8 Tf [(All studies were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory )] TJ ET BT 26.250 210.444 Td /F1 9.8 Tf [(Animals, NRC 2011. The protocol was approved by the Institutional Animal Care and Use Committee of Psychogenics, Inc. )] TJ ET BT 26.250 198.539 Td /F1 9.8 Tf [(\(PHS OLAW animal welfare assurance number A4471-01\), an AAALAC International accredited institution \(Unit #001213\).)] TJ ET BT 26.250 179.134 Td /F5 9.8 Tf [(Equipment)] TJ ET BT 26.250 159.729 Td /F1 9.8 Tf [(Mice were tested in standard mouse operant chambers \(Med Associates, VT\) with the floor area measuring 8.5? long x 7.0? )] TJ ET BT 26.250 147.825 Td /F1 9.8 Tf [(wide and 5.0? high walls. Each chamber contained a nosepoke recess, which could be illuminated by a small embedded bulb, )] TJ ET BT 26.250 135.920 Td /F1 9.8 Tf [(located centrally on the wall opposite the food magazine, though these were not employed in this testing. The boxes also )] TJ ET BT 26.250 124.015 Td /F1 9.8 Tf [(contained two retractable levers, one on either side of the food magazine. The chambers were located within individual sound )] TJ ET BT 26.250 112.110 Td /F1 9.8 Tf [(attenuating shells, with a fan mounted at one end of the sound-attenuating cubicle which was active throughout. Reinforcement )] TJ ET BT 26.250 100.206 Td /F1 9.8 Tf [(was provided by time-limited access to 0.01 ml of evaporated milk \(Carnation\) delivered via a dipper. The hardware was )] TJ ET BT 26.250 88.301 Td /F1 9.8 Tf [(controlled and all events were recorded by the Med-PC IV software package.)] TJ ET BT 26.250 68.896 Td /F5 9.8 Tf [(Behavioral procedures pretraining)] TJ ET BT 26.250 49.491 Td /F1 9.8 Tf [(Following food restriction and magazine training, all animals were trained to lever press via a simple free operant procedure, )] TJ ET BT 26.250 37.587 Td /F1 9.8 Tf [(where a single lever was inserted throughout a 40 min session and lever pressing was reinforced with 4-s access to an )] TJ ET Q q 15.000 23.301 577.500 753.699 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(Historically, researchers have utilized simple fixed-ratio schedules of reinforcement \(unchanging response requirement, typically )] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(at a low effort level\) or even simpler dose-response preference of rewards such as sucrose to measure consummatory )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(behaviors )] TJ ET 0.267 0.267 0.267 rg BT 71.227 725.769 Td /F4 8.7 Tf [(12)] TJ ET 0.271 0.267 0.267 rg BT 80.864 724.262 Td /F1 9.8 Tf [(. By contrast, progressive-ratio schedules \(increasing number of responses are required to obtain consecutive )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(deliveries of the reinforcer )] TJ ET 0.267 0.267 0.267 rg BT 141.125 713.864 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 150.762 712.357 Td /F1 9.8 Tf [(\), have been used to provide a more reliable and unambiguous measure of incentive motivation )] TJ ET 0.267 0.267 0.267 rg BT 564.250 713.864 Td /F4 8.7 Tf [(12)] TJ ET BT 26.250 701.960 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 35.887 700.452 Td /F1 9.8 Tf [(, in addition to providing a measure of reinforcer magnitude/efficacy. Typically, the final response requirement completed in )] TJ ET BT 26.250 688.548 Td /F1 9.8 Tf [(the test session is referred to as the break-point and has been used as the single performance measure. Recently however, )] TJ ET BT 26.250 676.643 Td /F1 9.8 Tf [(some have proposed the use of response rates at specific response ratios as a useful and informative output measure )] TJ ET 0.267 0.267 0.267 rg BT 537.832 678.150 Td /F4 8.7 Tf [(14)] TJ ET 0.271 0.267 0.267 rg BT 26.250 664.738 Td /F1 9.8 Tf [(although there is a risk of potential confounds with motor deficits if appropriate controls are not included in the experimental )] TJ ET BT 26.250 652.833 Td /F1 9.8 Tf [(design. Satiation is a potential confounding factor in this type of study, although the problem is much reduced under PR versus )] TJ ET BT 26.250 640.929 Td /F1 9.8 Tf [(FR schedules since delivery of reinforcers is typically far more limited under PR because of increasing response requirements.)] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(The motivation for the present experiments is to explore operant schedules of reinforcement to define an homologous )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(behavioral endpoint of the apathy symptom in the mouse, which, coupled with the relatively large number of preclinical HD )] TJ ET BT 26.250 597.714 Td /F1 9.8 Tf [(models now available )] TJ ET 0.267 0.267 0.267 rg BT 122.161 599.222 Td /F4 8.7 Tf [(15)] TJ ET BT 131.798 599.222 Td /F4 8.7 Tf [(16)] TJ ET BT 141.435 599.222 Td /F4 8.7 Tf [(17)] TJ ET BT 151.073 599.222 Td /F4 8.7 Tf [(18)] TJ ET BT 160.710 599.222 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 170.347 597.714 Td /F1 9.8 Tf [(, would be of great value for screening of possible therapeutics. Therefore, a mixed low effort )] TJ ET BT 26.250 585.810 Td /F1 9.8 Tf [(fixed- and escalating- effort progressive-ratio \(FR and PR, respectively\) procedure was utilized to explore consummatory )] TJ ET BT 26.250 573.905 Td /F1 9.8 Tf [(behavior \(relevant to immediate rewarding or reinforcing properties of a stimulus with the FR component\), and incentive )] TJ ET BT 26.250 562.000 Td /F1 9.8 Tf [(motivation \(PR component\). Two mouse models of HD were characterized in the task; specifically, the BAC HD full-length )] TJ ET BT 26.250 550.095 Td /F1 9.8 Tf [(transgenic model generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 222.410 551.603 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 232.048 550.095 Td /F1 9.8 Tf [(, and the z_Q175 knock-in model generated at PsychoGenics Inc. )] TJ ET 0.267 0.267 0.267 rg BT 518.737 551.603 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 528.374 550.095 Td /F1 9.8 Tf [(. As far as )] TJ ET BT 26.250 538.191 Td /F1 9.8 Tf [(these authors are aware, neither mouse has been shown to exhibit an apathetic phenotype. It was predicted that an HD mouse )] TJ ET BT 26.250 526.286 Td /F1 9.8 Tf [(model with an apathetic phenotype will reduce its work rate earlier in the test session, at a lower ratio requirement, than will a )] TJ ET BT 26.250 514.381 Td /F1 9.8 Tf [(healthy WT.)] TJ ET BT 26.250 477.779 Td /F4 12.0 Tf [(Experiment 1A)] TJ ET BT 26.250 457.824 Td /F1 9.8 Tf [(The initial experiments were conducted using adult BAC HD mice, a full-length transgenic model developed in the laboratory of )] TJ ET BT 26.250 445.920 Td /F1 9.8 Tf [(X. William Yang )] TJ ET 0.267 0.267 0.267 rg BT 97.766 447.427 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 26.250 426.515 Td /F5 9.8 Tf [(Materials and Methods)] TJ ET BT 26.250 407.110 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 387.705 Td /F1 9.8 Tf [(WT and transgenic BAC mice \(BAC HD, CHDI-007-\(3\)\(1\)\) were generated crossing dams carrying ~97 stable CAGCAA repeats )] TJ ET BT 26.250 375.801 Td /F1 9.8 Tf [(on a C57Bl/6J with FVB/NJ male mice at the Jackson Laboratory \(Bar Harbor, ME\) and shipped to the test facility as adults. )] TJ ET BT 26.250 363.896 Td /F1 9.8 Tf [(Mice were maintained in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to water.)] TJ ET BT 26.250 344.491 Td /F1 9.8 Tf [(Once acclimated to our colony, the animals were pair-housed and food restricted, with WT mice reduced to 85% of their free-)] TJ ET BT 26.250 332.586 Td /F1 9.8 Tf [(feeding body weights. The BAC Tg mice, which develop excess fat deposits, were food-restricted gradually to the point where a )] TJ ET BT 26.250 320.682 Td /F1 9.8 Tf [(matched control group consumed comparable amounts of food to 85% WT animals in a 30-min free feeding test, at which point )] TJ ET BT 26.250 308.777 Td /F1 9.8 Tf [(they were slightly heavier than controls \(mean 36.5 g for BAC mice, 28.1 g for WT controls on the initial day of training\). Mice )] TJ ET BT 26.250 296.872 Td /F1 9.8 Tf [(were maintained at their new target weights by daily provision of limited quantities of food \(BIO-SERV 500mg pellets\).)] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(A single group of female mice was evaluated, 8 animals per genotype. The mice had previously been evaluated in the )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(PhenoCube \(described in )] TJ ET 0.267 0.267 0.267 rg BT 138.970 267.070 Td /F4 8.7 Tf [(20)] TJ ET 0.271 0.267 0.267 rg BT 148.607 265.563 Td /F1 9.8 Tf [(\), an automated phenotyping system, but were nave to operant boxes and had never before been )] TJ ET BT 26.250 253.658 Td /F1 9.8 Tf [(food restricted. All mice were 73 weeks old 1 week on the initial day of instrumental training and were 79 weeks old 1 week )] TJ ET BT 26.250 241.753 Td /F1 9.8 Tf [(on the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 222.348 Td /F1 9.8 Tf [(All studies were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory )] TJ ET BT 26.250 210.444 Td /F1 9.8 Tf [(Animals, NRC 2011. The protocol was approved by the Institutional Animal Care and Use Committee of Psychogenics, Inc. )] TJ ET BT 26.250 198.539 Td /F1 9.8 Tf [(\(PHS OLAW animal welfare assurance number A4471-01\), an AAALAC International accredited institution \(Unit #001213\).)] TJ ET BT 26.250 179.134 Td /F5 9.8 Tf [(Equipment)] TJ ET BT 26.250 159.729 Td /F1 9.8 Tf [(Mice were tested in standard mouse operant chambers \(Med Associates, VT\) with the floor area measuring 8.5? long x 7.0? )] TJ ET BT 26.250 147.825 Td /F1 9.8 Tf [(wide and 5.0? high walls. Each chamber contained a nosepoke recess, which could be illuminated by a small embedded bulb, )] TJ ET BT 26.250 135.920 Td /F1 9.8 Tf [(located centrally on the wall opposite the food magazine, though these were not employed in this testing. The boxes also )] TJ ET BT 26.250 124.015 Td /F1 9.8 Tf [(contained two retractable levers, one on either side of the food magazine. The chambers were located within individual sound )] TJ ET BT 26.250 112.110 Td /F1 9.8 Tf [(attenuating shells, with a fan mounted at one end of the sound-attenuating cubicle which was active throughout. Reinforcement )] TJ ET BT 26.250 100.206 Td /F1 9.8 Tf [(was provided by time-limited access to 0.01 ml of evaporated milk \(Carnation\) delivered via a dipper. The hardware was )] TJ ET BT 26.250 88.301 Td /F1 9.8 Tf [(controlled and all events were recorded by the Med-PC IV software package.)] TJ ET BT 26.250 68.896 Td /F5 9.8 Tf [(Behavioral procedures pretraining)] TJ ET BT 26.250 49.491 Td /F1 9.8 Tf [(Following food restriction and magazine training, all animals were trained to lever press via a simple free operant procedure, )] TJ ET BT 26.250 37.587 Td /F1 9.8 Tf [(where a single lever was inserted throughout a 40 min session and lever pressing was reinforced with 4-s access to an )] TJ ET Q q 15.000 23.301 577.500 753.699 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(Historically, researchers have utilized simple fixed-ratio schedules of reinforcement \(unchanging response requirement, typically )] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(at a low effort level\) or even simpler dose-response preference of rewards such as sucrose to measure consummatory )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(behaviors )] TJ ET 0.267 0.267 0.267 rg BT 71.227 725.769 Td /F4 8.7 Tf [(12)] TJ ET 0.271 0.267 0.267 rg BT 80.864 724.262 Td /F1 9.8 Tf [(. By contrast, progressive-ratio schedules \(increasing number of responses are required to obtain consecutive )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(deliveries of the reinforcer )] TJ ET 0.267 0.267 0.267 rg BT 141.125 713.864 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 150.762 712.357 Td /F1 9.8 Tf [(\), have been used to provide a more reliable and unambiguous measure of incentive motivation )] TJ ET 0.267 0.267 0.267 rg BT 564.250 713.864 Td /F4 8.7 Tf [(12)] TJ ET BT 26.250 701.960 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 35.887 700.452 Td /F1 9.8 Tf [(, in addition to providing a measure of reinforcer magnitude/efficacy. Typically, the final response requirement completed in )] TJ ET BT 26.250 688.548 Td /F1 9.8 Tf [(the test session is referred to as the break-point and has been used as the single performance measure. Recently however, )] TJ ET BT 26.250 676.643 Td /F1 9.8 Tf [(some have proposed the use of response rates at specific response ratios as a useful and informative output measure )] TJ ET 0.267 0.267 0.267 rg BT 537.832 678.150 Td /F4 8.7 Tf [(14)] TJ ET 0.271 0.267 0.267 rg BT 26.250 664.738 Td /F1 9.8 Tf [(although there is a risk of potential confounds with motor deficits if appropriate controls are not included in the experimental )] TJ ET BT 26.250 652.833 Td /F1 9.8 Tf [(design. Satiation is a potential confounding factor in this type of study, although the problem is much reduced under PR versus )] TJ ET BT 26.250 640.929 Td /F1 9.8 Tf [(FR schedules since delivery of reinforcers is typically far more limited under PR because of increasing response requirements.)] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(The motivation for the present experiments is to explore operant schedules of reinforcement to define an homologous )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(behavioral endpoint of the apathy symptom in the mouse, which, coupled with the relatively large number of preclinical HD )] TJ ET BT 26.250 597.714 Td /F1 9.8 Tf [(models now available )] TJ ET 0.267 0.267 0.267 rg BT 122.161 599.222 Td /F4 8.7 Tf [(15)] TJ ET BT 131.798 599.222 Td /F4 8.7 Tf [(16)] TJ ET BT 141.435 599.222 Td /F4 8.7 Tf [(17)] TJ ET BT 151.073 599.222 Td /F4 8.7 Tf [(18)] TJ ET BT 160.710 599.222 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 170.347 597.714 Td /F1 9.8 Tf [(, would be of great value for screening of possible therapeutics. Therefore, a mixed low effort )] TJ ET BT 26.250 585.810 Td /F1 9.8 Tf [(fixed- and escalating- effort progressive-ratio \(FR and PR, respectively\) procedure was utilized to explore consummatory )] TJ ET BT 26.250 573.905 Td /F1 9.8 Tf [(behavior \(relevant to immediate rewarding or reinforcing properties of a stimulus with the FR component\), and incentive )] TJ ET BT 26.250 562.000 Td /F1 9.8 Tf [(motivation \(PR component\). Two mouse models of HD were characterized in the task; specifically, the BAC HD full-length )] TJ ET BT 26.250 550.095 Td /F1 9.8 Tf [(transgenic model generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 222.410 551.603 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 232.048 550.095 Td /F1 9.8 Tf [(, and the z_Q175 knock-in model generated at PsychoGenics Inc. )] TJ ET 0.267 0.267 0.267 rg BT 518.737 551.603 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 528.374 550.095 Td /F1 9.8 Tf [(. As far as )] TJ ET BT 26.250 538.191 Td /F1 9.8 Tf [(these authors are aware, neither mouse has been shown to exhibit an apathetic phenotype. It was predicted that an HD mouse )] TJ ET BT 26.250 526.286 Td /F1 9.8 Tf [(model with an apathetic phenotype will reduce its work rate earlier in the test session, at a lower ratio requirement, than will a )] TJ ET BT 26.250 514.381 Td /F1 9.8 Tf [(healthy WT.)] TJ ET BT 26.250 477.779 Td /F4 12.0 Tf [(Experiment 1A)] TJ ET BT 26.250 457.824 Td /F1 9.8 Tf [(The initial experiments were conducted using adult BAC HD mice, a full-length transgenic model developed in the laboratory of )] TJ ET BT 26.250 445.920 Td /F1 9.8 Tf [(X. William Yang )] TJ ET 0.267 0.267 0.267 rg BT 97.766 447.427 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 26.250 426.515 Td /F5 9.8 Tf [(Materials and Methods)] TJ ET BT 26.250 407.110 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 387.705 Td /F1 9.8 Tf [(WT and transgenic BAC mice \(BAC HD, CHDI-007-\(3\)\(1\)\) were generated crossing dams carrying ~97 stable CAGCAA repeats )] TJ ET BT 26.250 375.801 Td /F1 9.8 Tf [(on a C57Bl/6J with FVB/NJ male mice at the Jackson Laboratory \(Bar Harbor, ME\) and shipped to the test facility as adults. )] TJ ET BT 26.250 363.896 Td /F1 9.8 Tf [(Mice were maintained in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to water.)] TJ ET BT 26.250 344.491 Td /F1 9.8 Tf [(Once acclimated to our colony, the animals were pair-housed and food restricted, with WT mice reduced to 85% of their free-)] TJ ET BT 26.250 332.586 Td /F1 9.8 Tf [(feeding body weights. The BAC Tg mice, which develop excess fat deposits, were food-restricted gradually to the point where a )] TJ ET BT 26.250 320.682 Td /F1 9.8 Tf [(matched control group consumed comparable amounts of food to 85% WT animals in a 30-min free feeding test, at which point )] TJ ET BT 26.250 308.777 Td /F1 9.8 Tf [(they were slightly heavier than controls \(mean 36.5 g for BAC mice, 28.1 g for WT controls on the initial day of training\). Mice )] TJ ET BT 26.250 296.872 Td /F1 9.8 Tf [(were maintained at their new target weights by daily provision of limited quantities of food \(BIO-SERV 500mg pellets\).)] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(A single group of female mice was evaluated, 8 animals per genotype. The mice had previously been evaluated in the )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(PhenoCube \(described in )] TJ ET 0.267 0.267 0.267 rg BT 138.970 267.070 Td /F4 8.7 Tf [(20)] TJ ET 0.271 0.267 0.267 rg BT 148.607 265.563 Td /F1 9.8 Tf [(\), an automated phenotyping system, but were nave to operant boxes and had never before been )] TJ ET BT 26.250 253.658 Td /F1 9.8 Tf [(food restricted. All mice were 73 weeks old 1 week on the initial day of instrumental training and were 79 weeks old 1 week )] TJ ET BT 26.250 241.753 Td /F1 9.8 Tf [(on the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 222.348 Td /F1 9.8 Tf [(All studies were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory )] TJ ET BT 26.250 210.444 Td /F1 9.8 Tf [(Animals, NRC 2011. The protocol was approved by the Institutional Animal Care and Use Committee of Psychogenics, Inc. )] TJ ET BT 26.250 198.539 Td /F1 9.8 Tf [(\(PHS OLAW animal welfare assurance number A4471-01\), an AAALAC International accredited institution \(Unit #001213\).)] TJ ET BT 26.250 179.134 Td /F5 9.8 Tf [(Equipment)] TJ ET BT 26.250 159.729 Td /F1 9.8 Tf [(Mice were tested in standard mouse operant chambers \(Med Associates, VT\) with the floor area measuring 8.5? long x 7.0? )] TJ ET BT 26.250 147.825 Td /F1 9.8 Tf [(wide and 5.0? high walls. Each chamber contained a nosepoke recess, which could be illuminated by a small embedded bulb, )] TJ ET BT 26.250 135.920 Td /F1 9.8 Tf [(located centrally on the wall opposite the food magazine, though these were not employed in this testing. The boxes also )] TJ ET BT 26.250 124.015 Td /F1 9.8 Tf [(contained two retractable levers, one on either side of the food magazine. The chambers were located within individual sound )] TJ ET BT 26.250 112.110 Td /F1 9.8 Tf [(attenuating shells, with a fan mounted at one end of the sound-attenuating cubicle which was active throughout. Reinforcement )] TJ ET BT 26.250 100.206 Td /F1 9.8 Tf [(was provided by time-limited access to 0.01 ml of evaporated milk \(Carnation\) delivered via a dipper. The hardware was )] TJ ET BT 26.250 88.301 Td /F1 9.8 Tf [(controlled and all events were recorded by the Med-PC IV software package.)] TJ ET BT 26.250 68.896 Td /F5 9.8 Tf [(Behavioral procedures pretraining)] TJ ET BT 26.250 49.491 Td /F1 9.8 Tf [(Following food restriction and magazine training, all animals were trained to lever press via a simple free operant procedure, )] TJ ET BT 26.250 37.587 Td /F1 9.8 Tf [(where a single lever was inserted throughout a 40 min session and lever pressing was reinforced with 4-s access to an )] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(2)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 166 0 obj << /Type /Font /Subtype /Type1 /Name /F5 /BaseFont /Helvetica-Oblique /Encoding /WinAnsiEncoding >> endobj 167 0 obj << /Type /Annot /Subtype /Link /A 168 0 R /Border [0 0 0] /H /I /Rect [ 71.2268 724.9674 80.8641 733.7858 ] >> endobj 168 0 obj << /Type /Action >> endobj 169 0 obj << /Type /Annot /Subtype /Link /A 170 0 R /Border [0 0 0] /H /I /Rect [ 141.1245 713.0627 150.7618 721.8810 ] >> endobj 170 0 obj << /Type /Action >> endobj 171 0 obj << /Type /Annot /Subtype /Link /A 172 0 R /Border [0 0 0] /H /I /Rect [ 564.2496 713.0627 573.8869 721.8810 ] >> endobj 172 0 obj << /Type /Action >> endobj 173 0 obj << /Type /Annot /Subtype /Link /A 174 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 701.1579 35.8873 709.9762 ] >> endobj 174 0 obj << /Type /Action >> endobj 175 0 obj << /Type /Annot /Subtype /Link /A 176 0 R /Border [0 0 0] /H /I /Rect [ 537.8325 677.3484 547.4698 686.1667 ] >> endobj 176 0 obj << /Type /Action >> endobj 177 0 obj << /Type /Annot /Subtype /Link /A 178 0 R /Border [0 0 0] /H /I /Rect [ 122.1607 598.4199 131.7981 607.2382 ] >> endobj 178 0 obj << /Type /Action >> endobj 179 0 obj << /Type /Annot /Subtype /Link /A 180 0 R /Border [0 0 0] /H /I /Rect [ 131.7981 598.4199 141.4354 607.2382 ] >> endobj 180 0 obj << /Type /Action >> endobj 181 0 obj << /Type /Annot /Subtype /Link /A 182 0 R /Border [0 0 0] /H /I /Rect [ 141.4354 598.4199 151.0728 607.2382 ] >> endobj 182 0 obj << /Type /Action >> endobj 183 0 obj << /Type /Annot /Subtype /Link /A 184 0 R /Border [0 0 0] /H /I /Rect [ 151.0728 598.4199 160.7101 607.2382 ] >> endobj 184 0 obj << /Type /Action >> endobj 185 0 obj << /Type /Annot /Subtype /Link /A 186 0 R /Border [0 0 0] /H /I /Rect [ 160.7101 598.4199 170.3474 607.2382 ] >> endobj 186 0 obj << /Type /Action >> endobj 187 0 obj << /Type /Annot /Subtype /Link /A 188 0 R /Border [0 0 0] /H /I /Rect [ 222.4102 550.8009 232.0476 559.6192 ] >> endobj 188 0 obj << /Type /Action >> endobj 189 0 obj << /Type /Annot /Subtype /Link /A 190 0 R /Border [0 0 0] /H /I /Rect [ 518.7366 550.8009 528.3739 559.6192 ] >> endobj 190 0 obj << /Type /Action >> endobj 191 0 obj << /Type /Annot /Subtype /Link /A 192 0 R /Border [0 0 0] /H /I /Rect [ 97.7662 446.6252 107.4036 455.4435 ] >> endobj 192 0 obj << /Type /Action >> endobj 193 0 obj << /Type /Annot /Subtype /Link /A 194 0 R /Border [0 0 0] /H /I /Rect [ 138.9698 266.2682 148.6071 275.0865 ] >> endobj 194 0 obj << /Type /Action >> endobj 195 0 obj << /Type /Annot /Subtype /Link /A 196 0 R /Border [0 0 0] /H /I /Rect [ 71.2268 724.9674 80.8641 733.7858 ] >> endobj 196 0 obj << /Type /Action >> endobj 197 0 obj << /Type /Annot /Subtype /Link /A 198 0 R /Border [0 0 0] /H /I /Rect [ 141.1245 713.0627 150.7618 721.8810 ] >> endobj 198 0 obj << /Type /Action >> endobj 199 0 obj << /Type /Annot /Subtype /Link /A 200 0 R /Border [0 0 0] /H /I /Rect [ 564.2496 713.0627 573.8869 721.8810 ] >> endobj 200 0 obj << /Type /Action >> endobj 201 0 obj << /Type /Annot /Subtype /Link /A 202 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 701.1579 35.8873 709.9762 ] >> endobj 202 0 obj << /Type /Action >> endobj 203 0 obj << /Type /Annot /Subtype /Link /A 204 0 R /Border [0 0 0] /H /I /Rect [ 537.8325 677.3484 547.4698 686.1667 ] >> endobj 204 0 obj << /Type /Action >> endobj 205 0 obj << /Type /Annot /Subtype /Link /A 206 0 R /Border [0 0 0] /H /I /Rect [ 122.1607 598.4199 131.7981 607.2382 ] >> endobj 206 0 obj << /Type /Action >> endobj 207 0 obj << /Type /Annot /Subtype /Link /A 208 0 R /Border [0 0 0] /H /I /Rect [ 131.7981 598.4199 141.4354 607.2382 ] >> endobj 208 0 obj << /Type /Action >> endobj 209 0 obj << /Type /Annot /Subtype /Link /A 210 0 R /Border [0 0 0] /H /I /Rect [ 141.4354 598.4199 151.0728 607.2382 ] >> endobj 210 0 obj << /Type /Action >> endobj 211 0 obj << /Type /Annot /Subtype /Link /A 212 0 R /Border [0 0 0] /H /I /Rect [ 151.0728 598.4199 160.7101 607.2382 ] >> endobj 212 0 obj << /Type /Action >> endobj 213 0 obj << /Type /Annot /Subtype /Link /A 214 0 R /Border [0 0 0] /H /I /Rect [ 160.7101 598.4199 170.3474 607.2382 ] >> endobj 214 0 obj << /Type /Action >> endobj 215 0 obj << /Type /Annot /Subtype /Link /A 216 0 R /Border [0 0 0] /H /I /Rect [ 222.4102 550.8009 232.0476 559.6192 ] >> endobj 216 0 obj << /Type /Action >> endobj 217 0 obj << /Type /Annot /Subtype /Link /A 218 0 R /Border [0 0 0] /H /I /Rect [ 518.7366 550.8009 528.3739 559.6192 ] >> endobj 218 0 obj << /Type /Action >> endobj 219 0 obj << /Type /Annot /Subtype /Link /A 220 0 R /Border [0 0 0] /H /I /Rect [ 97.7662 446.6252 107.4036 455.4435 ] >> endobj 220 0 obj << /Type /Action >> endobj 221 0 obj << /Type /Annot /Subtype /Link /A 222 0 R /Border [0 0 0] /H /I /Rect [ 138.9698 266.2682 148.6071 275.0865 ] >> endobj 222 0 obj << /Type /Action >> endobj 223 0 obj << /Type /Annot /Subtype /Link /A 224 0 R /Border [0 0 0] /H /I /Rect [ 71.2268 724.9674 80.8641 733.7858 ] >> endobj 224 0 obj << /Type /Action >> endobj 225 0 obj << /Type /Annot /Subtype /Link /A 226 0 R /Border [0 0 0] /H /I /Rect [ 141.1245 713.0627 150.7618 721.8810 ] >> endobj 226 0 obj << /Type /Action >> endobj 227 0 obj << /Type /Annot /Subtype /Link /A 228 0 R /Border [0 0 0] /H /I /Rect [ 564.2496 713.0627 573.8869 721.8810 ] >> endobj 228 0 obj << /Type /Action >> endobj 229 0 obj << /Type /Annot /Subtype /Link /A 230 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 701.1579 35.8873 709.9762 ] >> endobj 230 0 obj << /Type /Action >> endobj 231 0 obj << /Type /Annot /Subtype /Link /A 232 0 R /Border [0 0 0] /H /I /Rect [ 537.8325 677.3484 547.4698 686.1667 ] >> endobj 232 0 obj << /Type /Action >> endobj 233 0 obj << /Type /Annot /Subtype /Link /A 234 0 R /Border [0 0 0] /H /I /Rect [ 122.1607 598.4199 131.7981 607.2382 ] >> endobj 234 0 obj << /Type /Action >> endobj 235 0 obj << /Type /Annot /Subtype /Link /A 236 0 R /Border [0 0 0] /H /I /Rect [ 131.7981 598.4199 141.4354 607.2382 ] >> endobj 236 0 obj << /Type /Action >> endobj 237 0 obj << /Type /Annot /Subtype /Link /A 238 0 R /Border [0 0 0] /H /I /Rect [ 141.4354 598.4199 151.0728 607.2382 ] >> endobj 238 0 obj << /Type /Action >> endobj 239 0 obj << /Type /Annot /Subtype /Link /A 240 0 R /Border [0 0 0] /H /I /Rect [ 151.0728 598.4199 160.7101 607.2382 ] >> endobj 240 0 obj << /Type /Action >> endobj 241 0 obj << /Type /Annot /Subtype /Link /A 242 0 R /Border [0 0 0] /H /I /Rect [ 160.7101 598.4199 170.3474 607.2382 ] >> endobj 242 0 obj << /Type /Action >> endobj 243 0 obj << /Type /Annot /Subtype /Link /A 244 0 R /Border [0 0 0] /H /I /Rect [ 222.4102 550.8009 232.0476 559.6192 ] >> endobj 244 0 obj << /Type /Action >> endobj 245 0 obj << /Type /Annot /Subtype /Link /A 246 0 R /Border [0 0 0] /H /I /Rect [ 518.7366 550.8009 528.3739 559.6192 ] >> endobj 246 0 obj << /Type /Action >> endobj 247 0 obj << /Type /Annot /Subtype /Link /A 248 0 R /Border [0 0 0] /H /I /Rect [ 97.7662 446.6252 107.4036 455.4435 ] >> endobj 248 0 obj << /Type /Action >> endobj 249 0 obj << /Type /Annot /Subtype /Link /A 250 0 R /Border [0 0 0] /H /I /Rect [ 138.9698 266.2682 148.6071 275.0865 ] >> endobj 250 0 obj << /Type /Action >> endobj 251 0 obj << /Type /Page /Parent 3 0 R /Annots [ 253 0 R 257 0 R 261 0 R ] /Contents 252 0 R >> endobj 252 0 obj << /Length 22328 >> stream 0.271 0.267 0.267 rg q 15.000 33.536 577.500 743.464 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(evaporated milk reinforcer on a response-initiated fixed-interval 20 sec \(FI20\) schedule. No reinforcement was delivered without )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(a lever press. Animals were trained to a criterion, requiring them to obtain 50 reinforcers across 2 consecutive sessions. )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(Training was carried out daily Monday to Friday, with the animals resting over the weekends, and continued until all test mice )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(had reached the criterion. Following criterion achievement, the animals were switched to an FR5 reinforcement schedule and )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(trained for a further 3 sessions, with the sessions lasting either for 40 min or until the animal earned 75 reinforcers, whichever )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(occurred soonest.)] TJ ET BT 26.250 688.548 Td /F5 9.8 Tf [(Behavioral procedures PR testing)] TJ ET BT 26.250 669.143 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 669.143 Td /F1 9.8 Tf [(Following completion of this training, the animals were switched to PR training, with the number of responses )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(required to earn a reward escalating steeply as the session progressed. The ratio sequence employed in these studies required )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(animals to make the following number of responses for each consecutive reinforcer: 1, 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(120, 140, 180, 220, 260, 300, 380, 460, 540, 620, 780, 940, 1100, 1260, 1580, 1900. These sessions continued either until the )] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(animals made no responses for at least 5 min, or until the session had continued for 90 min. This training continued for 26 )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(sessions.)] TJ ET BT 26.250 590.214 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 590.214 Td /F1 9.8 Tf [( Finally, the animals were switched to a mixed FR5/PR schedule, designed to measure their responding on a low-)] TJ ET BT 26.250 578.310 Td /F1 9.8 Tf [(effort \(the FR5\) and an escalating effort \(the PR\) schedule within a given test session. These sessions opened with 10 min of )] TJ ET BT 26.250 566.405 Td /F1 9.8 Tf [(training on the FR5 schedule, with no reinforcer cap, then continued for a further 80 min with the same PR schedule as )] TJ ET BT 26.250 554.500 Td /F1 9.8 Tf [(previously. This testing continued for 24 sessions.)] TJ ET BT 26.250 535.095 Td /F1 9.8 Tf [(During FR5/PR training in Experiment 1A, the animals inadvertently received an extra session of FR5-alone training between )] TJ ET BT 26.250 523.191 Td /F1 9.8 Tf [(sessions 7 and 8 due to experimenter error. Data from this session were discarded.)] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(Statistical methods)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(Data were evaluated via repeated measures analysis carried out with SAS \(SAS Institute Inc.\) using Mixed Effect Models, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(based on likelihood estimation. The models were fitted using the procedure PROC MIXED [21]. Genotype and one of Session, )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(Reinforcer Bin or Response Requirement, depending on the particular dataset, and their interactions were considered in all the )] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(models, with sex evaluated separately where appropriate. Significant interactions were followed up with simple main effects )] TJ ET BT 26.250 436.762 Td /F1 9.8 Tf [(analyses. Analysis of pretraining and response rate data was carried out with t-tests or one-way ANOVAs using Statview \(SAS )] TJ ET BT 26.250 424.857 Td /F1 9.8 Tf [(Institute Inc.\). An effect was considered significant if p < 0.05.)] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(Power analysis was performed using PASS11 version 11.0.5 from NCSS, LLC, using Repeated Measures ANOVA \(proc. )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(#166\). The goal was to find the minimum number of subjects per group required to obtain a power = 0.80 for a significant )] TJ ET BT 26.250 381.643 Td /F1 9.8 Tf [(statistical effect for several potential therapeutic effect sizes.)] TJ ET BT 26.250 362.238 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 342.834 Td /F5 9.8 Tf [(Pretraining)] TJ ET BT 73.391 342.834 Td /F1 9.8 Tf [(. All animals in this study readily acquired the lever press response, passing the reinforcer criterion in the initial two )] TJ ET BT 26.250 330.929 Td /F1 9.8 Tf [(days of testing. The majority of the mice obtained the maximum 75 reinforcers in each session of FR5 training, so no analysis )] TJ ET BT 26.250 319.024 Td /F1 9.8 Tf [(was attempted of their overall number of responses. There were no significant changes in response rates across the three test )] TJ ET BT 26.250 307.119 Td /F1 9.8 Tf [(sessions or differences between the two genotypes.)] TJ ET BT 26.250 287.715 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 287.715 Td /F1 9.8 Tf [(Data from the progressive ratio only test sessions \(26 sessions\) is presented in the left panel of Figure 1, below, and )] TJ ET BT 26.250 275.810 Td /F1 9.8 Tf [(clearly indicates that the BAC HD mice \(filled symbols\) earned fewer reinforcers than did WT controls \(open symbols\) )] TJ ET BT 26.250 263.905 Td /F1 9.8 Tf [(throughout training \(Genotype main effect: F\(1,13\) = 46.2, )] TJ ET BT 278.531 263.905 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 324.883 263.905 Td /F1 9.8 Tf [(; Session main effect: F\(25,350\) = 3.11,)] TJ ET BT 495.868 263.905 Td /F5 9.8 Tf [( p < 0.0001)] TJ ET BT 544.930 263.905 Td /F1 9.8 Tf [(; Trend )] TJ ET BT 26.250 252.000 Td /F1 9.8 Tf [(towards a Genotype x Session interaction: F\(25,350\) = 1.50, )] TJ ET BT 289.363 252.000 Td /F5 9.8 Tf [(p < 0.06; )] TJ ET BT 330.294 252.000 Td /F1 9.8 Tf [(Figure 1\).)] TJ ET 0.965 0.965 0.965 rg 26.250 100.560 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 242.120 m 581.250 242.120 l 581.250 241.370 l 26.250 241.370 l f 26.250 100.560 m 581.250 100.560 l 581.250 101.310 l 26.250 101.310 l f q 225.000 0 0 84.750 35.250 147.620 cm /I4 Do Q q 35.250 111.810 537.000 29.810 re W n 0.271 0.267 0.267 rg BT 35.250 132.096 Td /F4 9.8 Tf [(Fig. 1: Number of reinforcers earned during progressive ratio training across sessions in Experiments 1A \(left )] TJ ET BT 35.250 120.191 Td /F4 9.8 Tf [(panel\) and 1B \(right panel\))] TJ ET Q BT 26.250 83.536 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 83.536 Td /F1 9.8 Tf [( While the data from this PR training are of clear interest, in the absence of clearly defined breakpoints in the )] TJ ET BT 26.250 71.631 Td /F1 9.8 Tf [(animals responding the possibility remains that these slower overall response rates in the BAC Tg mice might be related to )] TJ ET BT 26.250 59.727 Td /F1 9.8 Tf [(some motor impairment. Accordingly, data from the final mixed FR5/PR phase of this experiment is presented in the left panels )] TJ ET BT 26.250 47.822 Td /F1 9.8 Tf [(of Figure 2, split into low effort FR5 training \(upper panel\) and escalating effort PR training \(lower panel\). In the FR5 phase, both )] TJ ET Q q 15.000 33.536 577.500 743.464 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(evaporated milk reinforcer on a response-initiated fixed-interval 20 sec \(FI20\) schedule. No reinforcement was delivered without )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(a lever press. Animals were trained to a criterion, requiring them to obtain 50 reinforcers across 2 consecutive sessions. )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(Training was carried out daily Monday to Friday, with the animals resting over the weekends, and continued until all test mice )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(had reached the criterion. Following criterion achievement, the animals were switched to an FR5 reinforcement schedule and )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(trained for a further 3 sessions, with the sessions lasting either for 40 min or until the animal earned 75 reinforcers, whichever )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(occurred soonest.)] TJ ET BT 26.250 688.548 Td /F5 9.8 Tf [(Behavioral procedures PR testing)] TJ ET BT 26.250 669.143 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 669.143 Td /F1 9.8 Tf [(Following completion of this training, the animals were switched to PR training, with the number of responses )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(required to earn a reward escalating steeply as the session progressed. The ratio sequence employed in these studies required )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(animals to make the following number of responses for each consecutive reinforcer: 1, 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(120, 140, 180, 220, 260, 300, 380, 460, 540, 620, 780, 940, 1100, 1260, 1580, 1900. These sessions continued either until the )] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(animals made no responses for at least 5 min, or until the session had continued for 90 min. This training continued for 26 )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(sessions.)] TJ ET BT 26.250 590.214 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 590.214 Td /F1 9.8 Tf [( Finally, the animals were switched to a mixed FR5/PR schedule, designed to measure their responding on a low-)] TJ ET BT 26.250 578.310 Td /F1 9.8 Tf [(effort \(the FR5\) and an escalating effort \(the PR\) schedule within a given test session. These sessions opened with 10 min of )] TJ ET BT 26.250 566.405 Td /F1 9.8 Tf [(training on the FR5 schedule, with no reinforcer cap, then continued for a further 80 min with the same PR schedule as )] TJ ET BT 26.250 554.500 Td /F1 9.8 Tf [(previously. This testing continued for 24 sessions.)] TJ ET BT 26.250 535.095 Td /F1 9.8 Tf [(During FR5/PR training in Experiment 1A, the animals inadvertently received an extra session of FR5-alone training between )] TJ ET BT 26.250 523.191 Td /F1 9.8 Tf [(sessions 7 and 8 due to experimenter error. Data from this session were discarded.)] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(Statistical methods)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(Data were evaluated via repeated measures analysis carried out with SAS \(SAS Institute Inc.\) using Mixed Effect Models, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(based on likelihood estimation. The models were fitted using the procedure PROC MIXED [21]. Genotype and one of Session, )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(Reinforcer Bin or Response Requirement, depending on the particular dataset, and their interactions were considered in all the )] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(models, with sex evaluated separately where appropriate. Significant interactions were followed up with simple main effects )] TJ ET BT 26.250 436.762 Td /F1 9.8 Tf [(analyses. Analysis of pretraining and response rate data was carried out with t-tests or one-way ANOVAs using Statview \(SAS )] TJ ET BT 26.250 424.857 Td /F1 9.8 Tf [(Institute Inc.\). An effect was considered significant if p < 0.05.)] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(Power analysis was performed using PASS11 version 11.0.5 from NCSS, LLC, using Repeated Measures ANOVA \(proc. )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(#166\). The goal was to find the minimum number of subjects per group required to obtain a power = 0.80 for a significant )] TJ ET BT 26.250 381.643 Td /F1 9.8 Tf [(statistical effect for several potential therapeutic effect sizes.)] TJ ET BT 26.250 362.238 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 342.834 Td /F5 9.8 Tf [(Pretraining)] TJ ET BT 73.391 342.834 Td /F1 9.8 Tf [(. All animals in this study readily acquired the lever press response, passing the reinforcer criterion in the initial two )] TJ ET BT 26.250 330.929 Td /F1 9.8 Tf [(days of testing. The majority of the mice obtained the maximum 75 reinforcers in each session of FR5 training, so no analysis )] TJ ET BT 26.250 319.024 Td /F1 9.8 Tf [(was attempted of their overall number of responses. There were no significant changes in response rates across the three test )] TJ ET BT 26.250 307.119 Td /F1 9.8 Tf [(sessions or differences between the two genotypes.)] TJ ET BT 26.250 287.715 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 287.715 Td /F1 9.8 Tf [(Data from the progressive ratio only test sessions \(26 sessions\) is presented in the left panel of Figure 1, below, and )] TJ ET BT 26.250 275.810 Td /F1 9.8 Tf [(clearly indicates that the BAC HD mice \(filled symbols\) earned fewer reinforcers than did WT controls \(open symbols\) )] TJ ET BT 26.250 263.905 Td /F1 9.8 Tf [(throughout training \(Genotype main effect: F\(1,13\) = 46.2, )] TJ ET BT 278.531 263.905 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 324.883 263.905 Td /F1 9.8 Tf [(; Session main effect: F\(25,350\) = 3.11,)] TJ ET BT 495.868 263.905 Td /F5 9.8 Tf [( p < 0.0001)] TJ ET BT 544.930 263.905 Td /F1 9.8 Tf [(; Trend )] TJ ET BT 26.250 252.000 Td /F1 9.8 Tf [(towards a Genotype x Session interaction: F\(25,350\) = 1.50, )] TJ ET BT 289.363 252.000 Td /F5 9.8 Tf [(p < 0.06; )] TJ ET BT 330.294 252.000 Td /F1 9.8 Tf [(Figure 1\).)] TJ ET 0.965 0.965 0.965 rg 26.250 100.560 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 242.120 m 581.250 242.120 l 581.250 241.370 l 26.250 241.370 l f 26.250 100.560 m 581.250 100.560 l 581.250 101.310 l 26.250 101.310 l f q 225.000 0 0 84.750 35.250 147.620 cm /I6 Do Q q 35.250 111.810 537.000 29.810 re W n 0.271 0.267 0.267 rg BT 35.250 132.096 Td /F4 9.8 Tf [(Fig. 1: Number of reinforcers earned during progressive ratio training across sessions in Experiments 1A \(left )] TJ ET BT 35.250 120.191 Td /F4 9.8 Tf [(panel\) and 1B \(right panel\))] TJ ET Q BT 26.250 83.536 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 83.536 Td /F1 9.8 Tf [( While the data from this PR training are of clear interest, in the absence of clearly defined breakpoints in the )] TJ ET BT 26.250 71.631 Td /F1 9.8 Tf [(animals responding the possibility remains that these slower overall response rates in the BAC Tg mice might be related to )] TJ ET BT 26.250 59.727 Td /F1 9.8 Tf [(some motor impairment. Accordingly, data from the final mixed FR5/PR phase of this experiment is presented in the left panels )] TJ ET BT 26.250 47.822 Td /F1 9.8 Tf [(of Figure 2, split into low effort FR5 training \(upper panel\) and escalating effort PR training \(lower panel\). In the FR5 phase, both )] TJ ET Q q 15.000 33.536 577.500 743.464 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(evaporated milk reinforcer on a response-initiated fixed-interval 20 sec \(FI20\) schedule. No reinforcement was delivered without )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(a lever press. Animals were trained to a criterion, requiring them to obtain 50 reinforcers across 2 consecutive sessions. )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(Training was carried out daily Monday to Friday, with the animals resting over the weekends, and continued until all test mice )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(had reached the criterion. Following criterion achievement, the animals were switched to an FR5 reinforcement schedule and )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(trained for a further 3 sessions, with the sessions lasting either for 40 min or until the animal earned 75 reinforcers, whichever )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(occurred soonest.)] TJ ET BT 26.250 688.548 Td /F5 9.8 Tf [(Behavioral procedures PR testing)] TJ ET BT 26.250 669.143 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 669.143 Td /F1 9.8 Tf [(Following completion of this training, the animals were switched to PR training, with the number of responses )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(required to earn a reward escalating steeply as the session progressed. The ratio sequence employed in these studies required )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(animals to make the following number of responses for each consecutive reinforcer: 1, 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(120, 140, 180, 220, 260, 300, 380, 460, 540, 620, 780, 940, 1100, 1260, 1580, 1900. These sessions continued either until the )] TJ ET BT 26.250 621.524 Td /F1 9.8 Tf [(animals made no responses for at least 5 min, or until the session had continued for 90 min. This training continued for 26 )] TJ ET BT 26.250 609.619 Td /F1 9.8 Tf [(sessions.)] TJ ET BT 26.250 590.214 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 590.214 Td /F1 9.8 Tf [( Finally, the animals were switched to a mixed FR5/PR schedule, designed to measure their responding on a low-)] TJ ET BT 26.250 578.310 Td /F1 9.8 Tf [(effort \(the FR5\) and an escalating effort \(the PR\) schedule within a given test session. These sessions opened with 10 min of )] TJ ET BT 26.250 566.405 Td /F1 9.8 Tf [(training on the FR5 schedule, with no reinforcer cap, then continued for a further 80 min with the same PR schedule as )] TJ ET BT 26.250 554.500 Td /F1 9.8 Tf [(previously. This testing continued for 24 sessions.)] TJ ET BT 26.250 535.095 Td /F1 9.8 Tf [(During FR5/PR training in Experiment 1A, the animals inadvertently received an extra session of FR5-alone training between )] TJ ET BT 26.250 523.191 Td /F1 9.8 Tf [(sessions 7 and 8 due to experimenter error. Data from this session were discarded.)] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(Statistical methods)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(Data were evaluated via repeated measures analysis carried out with SAS \(SAS Institute Inc.\) using Mixed Effect Models, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(based on likelihood estimation. The models were fitted using the procedure PROC MIXED [21]. Genotype and one of Session, )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(Reinforcer Bin or Response Requirement, depending on the particular dataset, and their interactions were considered in all the )] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(models, with sex evaluated separately where appropriate. Significant interactions were followed up with simple main effects )] TJ ET BT 26.250 436.762 Td /F1 9.8 Tf [(analyses. Analysis of pretraining and response rate data was carried out with t-tests or one-way ANOVAs using Statview \(SAS )] TJ ET BT 26.250 424.857 Td /F1 9.8 Tf [(Institute Inc.\). An effect was considered significant if p < 0.05.)] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(Power analysis was performed using PASS11 version 11.0.5 from NCSS, LLC, using Repeated Measures ANOVA \(proc. )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(#166\). The goal was to find the minimum number of subjects per group required to obtain a power = 0.80 for a significant )] TJ ET BT 26.250 381.643 Td /F1 9.8 Tf [(statistical effect for several potential therapeutic effect sizes.)] TJ ET BT 26.250 362.238 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 342.834 Td /F5 9.8 Tf [(Pretraining)] TJ ET BT 73.391 342.834 Td /F1 9.8 Tf [(. All animals in this study readily acquired the lever press response, passing the reinforcer criterion in the initial two )] TJ ET BT 26.250 330.929 Td /F1 9.8 Tf [(days of testing. The majority of the mice obtained the maximum 75 reinforcers in each session of FR5 training, so no analysis )] TJ ET BT 26.250 319.024 Td /F1 9.8 Tf [(was attempted of their overall number of responses. There were no significant changes in response rates across the three test )] TJ ET BT 26.250 307.119 Td /F1 9.8 Tf [(sessions or differences between the two genotypes.)] TJ ET BT 26.250 287.715 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 287.715 Td /F1 9.8 Tf [(Data from the progressive ratio only test sessions \(26 sessions\) is presented in the left panel of Figure 1, below, and )] TJ ET BT 26.250 275.810 Td /F1 9.8 Tf [(clearly indicates that the BAC HD mice \(filled symbols\) earned fewer reinforcers than did WT controls \(open symbols\) )] TJ ET BT 26.250 263.905 Td /F1 9.8 Tf [(throughout training \(Genotype main effect: F\(1,13\) = 46.2, )] TJ ET BT 278.531 263.905 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 324.883 263.905 Td /F1 9.8 Tf [(; Session main effect: F\(25,350\) = 3.11,)] TJ ET BT 495.868 263.905 Td /F5 9.8 Tf [( p < 0.0001)] TJ ET BT 544.930 263.905 Td /F1 9.8 Tf [(; Trend )] TJ ET BT 26.250 252.000 Td /F1 9.8 Tf [(towards a Genotype x Session interaction: F\(25,350\) = 1.50, )] TJ ET BT 289.363 252.000 Td /F5 9.8 Tf [(p < 0.06; )] TJ ET BT 330.294 252.000 Td /F1 9.8 Tf [(Figure 1\).)] TJ ET 0.965 0.965 0.965 rg 26.250 100.560 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 242.120 m 581.250 242.120 l 581.250 241.370 l 26.250 241.370 l f 26.250 100.560 m 581.250 100.560 l 581.250 101.310 l 26.250 101.310 l f q 225.000 0 0 84.750 35.250 147.620 cm /I8 Do Q q 35.250 111.810 537.000 29.810 re W n 0.271 0.267 0.267 rg BT 35.250 132.096 Td /F4 9.8 Tf [(Fig. 1: Number of reinforcers earned during progressive ratio training across sessions in Experiments 1A \(left )] TJ ET BT 35.250 120.191 Td /F4 9.8 Tf [(panel\) and 1B \(right panel\))] TJ ET Q BT 26.250 83.536 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 83.536 Td /F1 9.8 Tf [( While the data from this PR training are of clear interest, in the absence of clearly defined breakpoints in the )] TJ ET BT 26.250 71.631 Td /F1 9.8 Tf [(animals responding the possibility remains that these slower overall response rates in the BAC Tg mice might be related to )] TJ ET BT 26.250 59.727 Td /F1 9.8 Tf [(some motor impairment. Accordingly, data from the final mixed FR5/PR phase of this experiment is presented in the left panels )] TJ ET BT 26.250 47.822 Td /F1 9.8 Tf [(of Figure 2, split into low effort FR5 training \(upper panel\) and escalating effort PR training \(lower panel\). In the FR5 phase, both )] TJ ET Q q 225.000 0 0 84.750 35.250 147.620 cm /I10 Do Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(3)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 253 0 obj << /Type /Annot /Subtype /Link /A 254 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 147.6195 260.2500 232.3695 ] >> endobj 254 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure1.png) >> endobj 255 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 256 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 255 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 25625>> stream xgXG8KT+X@anl!1${IbhTĠ1"Q HGsv>~z^3wgݝ%)"Ii ` \ 0<X~eUF`U[J.\Aaάc9#%ߍ-[-*rGPzA`2z$8!,)YάR`|Y=3GtFFV7g8Kvt8>9l/Ht掄6+LS{WR񛏄5JpAʹ+kG$"hUxF ʾP~.\Z ix'9+mo7V!j+ .~DKJJD"(i8x`XXXDDMӭ6l`(Rɓ4MRQ\\! iũѣG۶m>|ɑdeeeR:##C&UTTTWW3%4M766|Q(mmmwmjjJHHx(߆ \oqÆ >ăxrXX^^_ bxxX9">\$ӧLOO‚ #F3&66v0rbbbssssssiii>}nݺ8rM6)))M<9(((//ٳgFFFb_SSɓʧN"Is̹vZ~~~ttǛݧOq!f͚}o>ٳgoذaӦM_nnn.**7nĉMLL/_ԔW^^^[[h"eڴi6m3g(Yz)S6m3pƺUZ0' ,kY1>>bssAܾ}{dd$… fffՌmmm2VU$eeeH{cǎϙ3'}AlllA/N:uӦMg%k֬qttܴi9DL988())577ڵqGqnnnNf--GSЇ%O5 1$ɖ]vȥ WN+ 8N)vwn)(~rAnT0J8uHgg ѵhooYS;뜐z7It0rbSb 貪NJ#\e\>rW@TH!>gV<|"wL\Bq=NEz@k~5"$@E@(ݻ髯B>=vxKAy{ w{_J`<"ME֛u.VH\{섞/2ExvmTVVڶkkkkkksi J, ZJ SbUUPP۷?^PP@Dyys{{;1pWQdJnwwr\q9\~Y(jQQmnݺܜ~lVWWWSS#*ED$OLH 48ޏ ~˅ ɚxJJJrsspsn޼y&Lx۷BBB֭[a܂gqg4 OVQ1kcU؈Ӓ$vU(;wY~ר(kkkG*++ gϞ.e+ٳrﰦ6Ӳa1 8x@Dj#ɓƍĕ.L;v֭["(88y---B%55U*Vs9{%/ J Bjx21]|WX ܹsT__ƍSLuppرcG@@@KKː!Cbbb̼~||>>ßsEGGXK<+B^<؂3'y,**(2r)rEP FiiiioaaW`Zm@O?577_|[n7׶EEEb8""o߾JJJ۷o_|]]],X~PPPЎ;hgp',@Dggqg`` Hd``@tiOOk׮;RCCcYYY?~9r!CF˷\r…̸K[ZZ$dT Q UEE۷'O}~uP ƍfeeyxxhii9;;Ϛ5k޼yVZ|5k֭[k׮K۷OEE͛7+W\)G8 >|6m ŷnݒA \yeff]6**jݺuCMHH4iePPEQ9OصkFTTm6lK/^|͝;wwƍ#GdڿL<͛7ϟ?:8::}/11qVVV.]ڵkɓ'B? ^#ɵeee***r}yAKKeܹ3g-^ -???##tϟܹ k׮|ٳg)S8::^xŋzJHHqF||P(433CI֭[߿ogg6i$CCCggg3f<~BIIi̙G=tЍ7 4vE9::z |O}믿f͚ ,++xW_}zj0gDAee>}yH$+++| য়~>|*,,TVV Y(ׯ_o߾̱O>b 7:u Fc%_ 6eV;;;===oݺCIR7;_555.\2H-dFGGggg]'lmmE"EQLJJz̙3w JKK۹sguu5EQgϞ(J*^xtMӝvyŋ冞>\ZZdcǎGDDl߾}ϟ?okkdrEY;.{bh͞3f̐{C?W- @__ԩ3=f̄ge `eee###>{3g߿Ĉ:::?f~~_￟8qe4My&..EWW˗/WUUݻW^rC@wޓܣ=^x'K@={v~~#G-[FQիW>߿~,YJtSSSMMP($I(.. twtt_~#Gwٳgp=YXWQe``iaa1dHdaaFQ})Aܹs'BaI'* *S277ge1W\QVV=z'ŅKId>|MӃ>qpΓ'O6o޼ut###SSӺ\r***GٴiŋwtnƃT__OA޽yL߼y6} 4㖖/BOO/00.111..ڵk K.%IrڴiW^MMM_ yM\\ܲeˤRiJJJJJʕ+>}Y2L 8ήt_$0sGs,O>#q a#<]X, ~𡃃… AW8p Azzz:::***C 9s@ ѳ hjj \g4dnn4&xj[[`SN]zuHHȝ;w?>ڿoAX[[XZZd?Z"رǏ͐$yٻwM:ѣG^۷/yyy/_Ojjj}qܹsooׯ_S|m6uuuǎ 1mmw?o߾}}L''/BEE...&&&C  L p|27$I^ʪw`u4[0J|Ǐk֬isȐ!/4=a„qƁ͎Fe˖!C̘1c„ wޕdyX4cbbbbbRRR"##mmm) /Żw={j*}Y[Zqq1xl :o<UV!NWWBGGӧ˖-311ikk3f M999{```cce[[X,޽{v={j%  Z Ǜ7%''4tǏO< SrwVVVT0=PRRXzG}}OVVI#F$YZZ IR&8q" ˗1%#I|T 2e & Xgll Mr a`ҤI9bxĈ$I:99޽~qP(ܳgϮ]~嗈+++OP8{쒒;;;V|L-ZTSSsȑ 6 'O0`ʦM벳˕H477_@&IR]],D$I4غ$>}M:uϞ=۶md4ijj7a„ӧ͜9uGWd2IC^$''GFF$YRRdzgϖ.]LQY@@s缽CBBTUUmfffW_ٳ'))}ɒ%QQQeee`ӧOgggK$t$4Id$ y eee9!EQOCpp0xAȽ{ pyRw%&&п5//<(cرAСC(xŅ>>> ֛7oRSS)***oKdeeQj*/]]]/_,H1 7Shّll7֭[BQ۷2Ο?Y[[˅9!(ƍGӦM377’GGG___kHQԥK(z왽s/_dggw]vHҲ20%EyEQ`dmm파 :;;O> <,YBQD"9uT{{;*TD"@vڀR{^!޽{Eeee=zhܸq6lx왓ÇwOOI&EGGU)S\xۛ!]VG;y7%m?閖և Ǐ?#x~ uuuHP vPYY"&MeggW^eff}7&MbB8v쇩ZUUyff>}:1lذW:99&7o޸Ӂ'NP% O:u[Aϟ?򊎎Gcd%K$ikk{}f_ L,o޼͛4Mo߾_~0bciicǒh ⴵ innniGM> ,pss[vٳ_~ $bɞ RRRꫯ.\%s]xҀΞ=3sm۶-Y4ĥ2Sֲ2߿=G,fY^^~ᶶ6DiӦZ@n:s ѱbŊcǎݹs+;aLLѣG޽ L'iŋǏ?{,0w=~J4 zȾ}̞ gggX,J$ @}+_ݨ(~WXrJеo@׮][RR nEH1b͛7KwE! W]p766_ _?...Ν;uuu !3\O$oܸ L&ӧϡCnذ!''gJOO/;;[*ڵk֭BÇZZZ6$$;w?#G433;px! eee&3F<0%)**JHH㇟_{{X,i:77wΝ/^i?ȏ{A̙3಴͛~~~%%%hUU͛7`uuuMMM'N([/Ahiiفq6@<[Bu}!(:::,, Ι3͛2??4M3طoD"d2/4MWTT,^_Ǝ &r+W7o^~~>I?޽P SSP*]2]UUu޼y9rή( w CD(Vxb%7o޻w@D"]5k$''SuU7F]]c@ջw'Eͼ'N~.ɓ+Vttt>|8!!aܸq)))aaa_74M/)) {nIKKɓ';gnnݻA',//?uԥK@#}ե2>>>ϟg߿/@aHHH8oÆ sqqaWZ[[l߾W^ .;v޽{mfeehoݺU__Ot~~>G9`p>}tp۷o0Rt-򒒒@CLII8q+++fMǍ,:;;4tĉV ^ ඁ($l9r ˟<())eѣG>>Ǐ;;;ܹ5i$fbCFA@&1(*$$СCE^t)22i~~~gpYPP$`5[]]DǏWNOOwppػw뛛R)$IC>D4t=\ L ߿ϼr]UUWWW? 2###AHMM ӧiHHt-g<_Fwvv۷LRԜ;;;XgU*-----u BfX=z/ )jnnXuuuA۷… T d0ߵkWee%`~7n0/ W߾} ػw/xBstE,>>|MN֭[4 G\ ~%4GABV92-xIfh߾}Ah"6lؐIQTFFP6€Ixeee'sss9].Û7owȮ[!33>>mmmAڒ$ fee:tH[[{_5A2ڵk+<4T\\lffcBBB~iӦ}G}(6)Gq!'W`߾} ʕ+nnnQQQb(### FyL7G.(SgVoVWW9rm33kGGFLBeÇ߿Y ֭?ϝgΜL£GrrrFP(MԿ(Z|Y@UpEݻ/^ˆt_.MQǏmllx 3-[l2֜ͻJJJ+W_=zpBCf\UU#33333311QYY2={fBkkkhh"c5e}FUgF\(N#ɦLd``wCYgd! _oaa1|p0"73Yܟ-r#r{"ƾSDO~R8&,eС`nhmmDѣG/^f͚777q%2U666`%QѬma,\p>|ѝꉌ \ng D))) f<}hH9~J`vteB5b?.MW+; s9h[ڮ]]svշhPbC\hhhHQ/L&kkk---577WUU8š[v, NA4#>'fΜVTTosss띝,5_*X?<Oϟ?m4 333{eff <āi|" ?;,nDBzʕ¢W^Çg6Չ.h`9SSa 7.=ց055u̘1W%VVVjjjMMMPxׯˍ l5 |Qsv֭߃򂂂Z[[ۈf~a˖-s5(<\[qqxb^ma}ꫯMMMA1ؠC Gb]'oHCX:6Vq$,U:[W fҤIyyyE++!C$9x`NKT D"c F[,Bj+m"h(iV*\-\5< JZʙ专!0zs"WE eprwx!km6"]n$>\IIIOOQ fXCVws\U?:nŴtgU#W!>PdV~حi9| |)"~ɃƜ#]p9$/p3N'FrIL23=dZ^)ŠVBT<4rEH9T&s<x". K-!'_p _qaUk\rϟn?e @V)H[;irH+*b .uBaĴ-n<rVq49":-E`U qUbO~9gD{62pg˪""x x.Y>}脴fېreΚ0 n9kBIG31~ L!2h,rilJG B> BTO|7* ,wcۅ( >n9II\Tb5_'&H`Q$4ݘcꌛ~t$“(, 'j|0+ bul +NXBfnAT"<AK r\\09 .B\c2?*ELҿ(h!]j($_aё5]6zb$33q!P~777KKK :;;Aյkzh"plٲ;w󶶶۷_2 b8f\\۷ow.o~ASSS˗۶m7o>vX>}9v؅ @չsN3ԄK$̙3`Y 4My)'JOOy K.}Մ{TIRRRAA}B04"B{{NVWW7// ???''ӈ$I޼y3>>Qʹs熄MLLb1b޾}ZZZ*DRPPp՘DI oõ +W ( jii?D^hBQTuuuXXX $ѣ^ RMMMꪪ 0٩gnnP4- wѧOdϟjjj hG4hM?޽{8qbSSX-ـi Vhhh jooolllkkA|||rr2N֎hDpL&khh|2EQLeff D%%%^^^޽0TUU?yL`=kjj?~|-Sggg=5D{f9 ;w!<pqEݺu+++ OL(h! ,uT͛R!slnnNKK 0sHTWW][QMMMLL͛7Z:b~^^SRRtDRZZ啘S]]}ʕvD՚ϟ3 NOOJ0ݾ}޽ىPTTٳBLRlw42 -4M}6&&Lz$6$n߾ikkxɋ/:::C3EEGG?~8///==HI?+I***#F333dtvv~'O166|%KRޞ%I255wsioo766&۶" @b8p*C(J +++aA$''+))MII׏*djj g k9݁hL9ӧOp@}}}FaTѯ_?''' +99/ ``hh8}NKKKغJPHDFF ܖ(QSSJ4#ݻwzWSS#4===]]/^XXXpE 5C|tvv?(ǏR4777--mqqqv}UUU+V.~<%.>y򤼼o'.%&&w„ JKKV^B/JJJ6mx0UUUK?]{LS?K +( fL*ÍtqjqY6c%5%KtLp.LT  bݠ\z NK=9{}}s?sP(;( Rt8[n%vrrrlll||xF$RV!'?4LL&SRutt|z~׮]̌4===VH$A^xt:鬭ټy3njooLJJƱ 2@ `0 ,ݻAbJr8l6^#4:`0=zL&{Q{.8J$Yt:]b"H((Lx#lllvZ$YzX,)(t7o2;wf`0n:X\\\ dl7oF"7r\]bT* FOOh4x)pWz]~۷Y,۷ T;w0 J̩K.x<!]|90LTB'd655 Aw&xp]/~‚c0/_0ܹp޻wOV߼y3fee ΫWj4ł5kJJJ0 Bϟ===PhÆ @VP 0 }r-l?#x<]]] (~%$$444kssG ݻw .qI8vŋ|>(D"r8k׮/ 3uLJ_}lQpF^.j|GQ411}A1CCCWZǎJLsrr֮]qFɞLfٜN~D8 7nBN> 6544L&344T]]!@ jH$t۶mKII^6778NB!pž~:҃OB`vywqu BReffTTTT*Br\'O` *=Rd\(z333/R~~-[*6]SSj9vڠ611ÇUUUCCC}P([11Lvkjjd2 E__T*jR_ONNF* Tvf'|@foAO: BCC8޻wL&U`)lV++++=jllH$`vԩ@ .4ɓ' @ @Q744 7::ZYYYPPE+77WզTUU}%Az lxaAAAEEťK*++ |ᇊ rW8zhwwwAA0 ۾};ñZ0~fY۷iii`WD~嗚Z- )h{E`*++:&--mZ6//oӦM2``fZ{{{}@ SRRLaؕ+WjjjD"ў={L& H]]ݮ]@ݴiH$)(-[h4LVVV q\rM(VWW'%%mUSB'<[roߞ (feeٳ7 8Ή'._ -A5MNN|jooOII)--o۶-;;l\.Www\.ݿtҡCFGGҪLL&Fر7 tvv(m6Z ΐYYYx:::r98tiiiL&Y__uV@ׯ_ wܩT*322xl&I.u#U+!! \ÇpF)#L$4D;]]]Qze~4FgwwမZ888Hj~~ T?sYz."ԒA OY/<9!,4w("N"OJ"؅gbuZdN¹XM&|7$4yJ 1r(3f45;huųâ7i(:O`̰,ɋ'2f(E'J#Y% 9yq&#%Ebhdt D"haQ:%ѐZ$ڑ({%,^ցRNp[x&94pBD ,,egad3h%dkcHa@I6nEU!PGv4 i"OU؅hqAR F3!y%P)~|A<, <nD"NOT8MOO   qi#60 BH1 Jbr !z~jj`0D Og+SJ420On VX8?kZY,RߏaHą4 f'%[xU? %Je8P*"]`KȡAV45侲Tdaa@2;;+ x<2fرCծZt졡!ܩ$cZ]RR:bw\LNN9sL`={d?ֺ\.]. "X811p8Ri0d2f9##crrjl\1))iffVQ&4lbjL466* A6lPZZd2O>bl7|VVV믿JwFcaaȈbّHv4KT/񍏏{<s&_X,dR)Z[[QdL&(r&&&5 ^j B# ~8_@bA,S%}!\ZZ # U*8<ϗv ^P(D"p82l͚5($%%9NX k˫۩/_cǎo655$33X~nnnjj*{WdJOO)Gƶq $7r\.#v8"`D峳%b$55喖B!$V+G@+VOTLPp^E[[[7or322FGG\ffy~~^$Fy9!9%SN6M$I$JnZJ$MOO㯫ʱ8>2R`X,*ÉĢ<_Ș}L`~ %-t ˰ޝx lp1hVK#v^\x܏hr(UP|`aM!8OL00~U_'gN?sQp<)}ړfS/cN0~7Ki^4 UыAqr馩GeՒ$W=[/aq *1U endstream endobj 257 0 obj << /Type /Annot /Subtype /Link /A 258 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 147.6195 260.2500 232.3695 ] >> endobj 258 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure1.png) >> endobj 259 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 260 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 259 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 25625>> stream xgXG8KT+X@anl!1${IbhTĠ1"Q HGsv>~z^3wgݝ%)"Ii ` \ 0<X~eUF`U[J.\Aaάc9#%ߍ-[-*rGPzA`2z$8!,)YάR`|Y=3GtFFV7g8Kvt8>9l/Ht掄6+LS{WR񛏄5JpAʹ+kG$"hUxF ʾP~.\Z ix'9+mo7V!j+ .~DKJJD"(i8x`XXXDDMӭ6l`(Rɓ4MRQ\\! iũѣG۶m>|ɑdeeeR:##C&UTTTWW3%4M766|Q(mmmwmjjJHHx(߆ \oqÆ >ăxrXX^^_ bxxX9">\$ӧLOO‚ #F3&66v0rbbbssssssiii>}nݺ8rM6)))M<9(((//ٳgFFFb_SSɓʧN"Is̹vZ~~~ttǛݧOq!f͚}o>ٳgoذaӦM_nnn.**7nĉMLL/_ԔW^^^[[h"eڴi6m3g(Yz)S6m3pƺUZ0' ,kY1>>bssAܾ}{dd$… fffՌmmm2VU$eeeH{cǎϙ3'}AlllA/N:uӦMg%k֬qttܴi9DL988())577ڵqGqnnnNf--GSЇ%O5 1$ɖ]vȥ WN+ 8N)vwn)(~rAnT0J8uHgg ѵhooYS;뜐z7It0rbSb 貪NJ#\e\>rW@TH!>gV<|"wL\Bq=NEz@k~5"$@E@(ݻ髯B>=vxKAy{ w{_J`<"ME֛u.VH\{섞/2ExvmTVVڶkkkkkksi J, ZJ SbUUPP۷?^PP@Dyys{{;1pWQdJnwwr\q9\~Y(jQQmnݺܜ~lVWWWSS#*ED$OLH 48ޏ ~˅ ɚxJJJrsspsn޼y&Lx۷BBB֭[a܂gqg4 OVQ1kcU؈Ӓ$vU(;wY~ר(kkkG*++ gϞ.e+ٳrﰦ6Ӳa1 8x@Dj#ɓƍĕ.L;v֭["(88y---B%55U*Vs9{%/ J Bjx21]|WX ܹsT__ƍSLuppرcG@@@KKː!Cbbb̼~||>>ßsEGGXK<+B^<؂3'y,**(2r)rEP FiiiioaaW`Zm@O?577_|[n7׶EEEb8""o߾JJJ۷o_|]]],X~PPPЎ;hgp',@Dggqg`` Hd``@tiOOk׮;RCCcYYY?~9r!CF˷\r…̸K[ZZ$dT Q UEE۷'O}~uP ƍfeeyxxhii9;;Ϛ5k޼yVZ|5k֭[k׮K۷OEE͛7+W\)G8 >|6m ŷnݒA \yeff]6**jݺuCMHH4iePPEQ9OصkFTTm6lK/^|͝;wwƍ#GdڿL<͛7ϟ?:8::}/11qVVV.]ڵkɓ'B? ^#ɵeee***r}yAKKeܹ3g-^ -???##tϟܹ k׮|ٳg)S8::^xŋzJHHqF||P(433CI֭[߿ogg6i$CCCggg3f<~BIIi̙G=tЍ7 4vE9::z |O}믿f͚ ,++xW_}zj0gDAee>}yH$+++| য়~>|*,,TVV Y(ׯ_o߾̱O>b 7:u Fc%_ 6eV;;;===oݺCIR7;_555.\2H-dFGGggg]'lmmE"EQLJJz̙3w JKK۹sguu5EQgϞ(J*^xtMӝvyŋ冞>\ZZdcǎGDDl߾}ϟ?okkdrEY;.{bh͞3f̐{C?W- @__ԩ3=f̄ge `eee###>{3g߿Ĉ:::?f~~_￟8qe4My&..EWW˗/WUUݻW^rC@wޓܣ=^x'K@={v~~#G-[FQիW>߿~,YJtSSSMMP($I(.. twtt_~#Gwٳgp=YXWQe``iaa1dHdaaFQ})Aܹs'BaI'* *S277ge1W\QVV=z'ŅKId>|MӃ>qpΓ'O6o޼ut###SSӺ\r***GٴiŋwtnƃT__OA޽yL߼y6} 4㖖/BOO/00.111..ڵk K.%IrڴiW^MMM_ yM\\ܲeˤRiJJJJJʕ+>}Y2L 8ήt_$0sGs,O>#q a#<]X, ~𡃃… AW8p Azzz:::***C 9s@ ѳ hjj \g4dnn4&xj[[`SN]zuHHȝ;w?>ڿoAX[[XZZd?Z"رǏ͐$yٻwM:ѣG^۷/yyy/_Ojjj}qܹsooׯ_S|m6uuuǎ 1mmw?o߾}}L''/BEE...&&&C  L p|27$I^ʪw`u4[0J|Ǐk֬isȐ!/4=a„qƁ͎Fe˖!C̘1c„ wޕdyX4cbbbbbRRR"##mmm) /Żw={j*}Y[Zqq1xl :o<UV!NWWBGGӧ˖-311ikk3f M999{```cce[[X,޽{v={j%  Z Ǜ7%''4tǏO< SrwVVVT0=PRRXzG}}OVVI#F$YZZ IR&8q" ˗1%#I|T 2e & Xgll Mr a`ҤI9bxĈ$I:99޽~qP(ܳgϮ]~嗈+++OP8{쒒;;;V|L-ZTSSsȑ 6 'O0`ʦM벳˕H477_@&IR]],D$I4غ$>}M:uϞ=۶md4ijj7a„ӧ͜9uGWd2IC^$''GFF$YRRdzgϖ.]LQY@@s缽CBBTUUmfffW_ٳ'))}ɒ%QQQeee`ӧOgggK$t$4Id$ y eee9!EQOCpp0xAȽ{ pyRw%&&п5//<(cرAСC(xŅ>>> ֛7oRSS)***oKdeeQj*/]]]/_,H1 7Shّll7֭[BQ۷2Ο?Y[[˅9!(ƍGӦM377’GGG___kHQԥK(z왽s/_dggw]vHҲ20%EyEQ`dmm파 :;;O> <,YBQD"9uT{{;*TD"@vڀR{^!޽{Eeee=zhܸq6lx왓ÇwOOI&EGGU)S\xۛ!]VG;y7%m?閖և Ǐ?#x~ uuuHP vPYY"&MeggW^eff}7&MbB8v쇩ZUUyff>}:1lذW:99&7o޸Ӂ'NP% O:u[Aϟ?򊎎Gcd%K$ikk{}f_ L,o޼͛4Mo߾_~0bciicǒh ⴵ innniGM> ,pss[vٳ_~ $bɞ RRRꫯ.\%s]xҀΞ=3sm۶-Y4ĥ2Sֲ2߿=G,fY^^~ᶶ6DiӦZ@n:s ѱbŊcǎݹs+;aLLѣG޽ L'iŋǏ?{,0w=~J4 zȾ}̞ gggX,J$ @}+_ݨ(~WXrJеo@׮][RR nEH1b͛7KwE! W]p766_ _?...Ν;uuu !3\O$oܸ L&ӧϡCnذ!''gJOO/;;[*ڵk֭BÇZZZ6$$;w?#G433;px! eee&3F<0%)**JHH㇟_{{X,i:77wΝ/^i?ȏ{A̙3಴͛~~~%%%hUU͛7`uuuMMM'N([/Ahiiفq6@<[Bu}!(:::,, Ι3͛2??4M3طoD"d2/4MWTT,^_Ǝ &r+W7o^~~>I?޽P SSP*]2]UUu޼y9rή( w CD(Vxb%7o޻w@D"]5k$''SuU7F]]c@ջw'Eͼ'N~.ɓ+Vttt>|8!!aܸq)))aaa_74M/)) {nIKKɓ';gnnݻA',//?uԥK@#}ե2>>>ϟg߿/@aHHH8oÆ sqqaWZ[[l߾W^ .;v޽{mfeehoݺU__Ot~~>G9`p>}tp۷o0Rt-򒒒@CLII8q+++fMǍ,:;;4tĉV ^ ඁ($l9r ˟<())eѣG>>Ǐ;;;ܹ5i$fbCFA@&1(*$$СCE^t)22i~~~gpYPP$`5[]]DǏWNOOwppػw뛛R)$IC>D4t=\ L ߿ϼr]UUWWW? 2###AHMM ӧiHHt-g<_Fwvv۷LRԜ;;;XgU*-----u BfX=z/ )jnnXuuuA۷… T d0ߵkWee%`~7n0/ W߾} ػw/xBstE,>>|MN֭[4 G\ ~%4GABV92-xIfh߾}Ah"6lؐIQTFFP6€Ixeee'sss9].Û7owȮ[!33>>mmmAڒ$ fee:tH[[{_5A2ڵk+<4T\\lffcBBB~iӦ}G}(6)Gq!'W`߾} ʕ+nnnQQQb(### FyL7G.(SgVoVWW9rm33kGGFLBeÇ߿Y ֭?ϝgΜL£GrrrFP(MԿ(Z|Y@UpEݻ/^ˆt_.MQǏmllx 3-[l2֜ͻJJJ+W_=zpBCf\UU#33333311QYY2={fBkkkhh"c5e}FUgF\(N#ɦLd``wCYgd! _oaa1|p0"73Yܟ-r#r{"ƾSDO~R8&,eС`nhmmDѣG/^f͚777q%2U666`%QѬma,\p>|ѝꉌ \ng D))) f<}hH9~J`vteB5b?.MW+; s9h[ڮ]]svշhPbC\hhhHQ/L&kkk---577WUU8š[v, NA4#>'fΜVTTosss띝,5_*X?<Oϟ?m4 333{eff <āi|" ?;,nDBzʕ¢W^Çg6Չ.h`9SSa 7.=ց055u̘1W%VVVjjjMMMPxׯˍ l5 |Qsv֭߃򂂂Z[[ۈf~a˖-s5(<\[qqxb^ma}ꫯMMMA1ؠC Gb]'oHCX:6Vq$,U:[W fҤIyyyE++!C$9x`NKT D"c F[,Bj+m"h(iV*\-\5< JZʙ专!0zs"WE eprwx!km6"]n$>\IIIOOQ fXCVws\U?:nŴtgU#W!>PdV~حi9| |)"~ɃƜ#]p9$/p3N'FrIL23=dZ^)ŠVBT<4rEH9T&s<x". K-!'_p _qaUk\rϟn?e @V)H[;irH+*b .uBaĴ-n<rVq49":-E`U qUbO~9gD{62pg˪""x x.Y>}脴fېreΚ0 n9kBIG31~ L!2h,rilJG B> BTO|7* ,wcۅ( >n9II\Tb5_'&H`Q$4ݘcꌛ~t$“(, 'j|0+ bul +NXBfnAT"<AK r\\09 .B\c2?*ELҿ(h!]j($_aё5]6zb$33q!P~777KKK :;;Aյkzh"plٲ;w󶶶۷_2 b8f\\۷ow.o~ASSS˗۶m7o>vX>}9v؅ @չsN3ԄK$̙3`Y 4My)'JOOy K.}Մ{TIRRRAA}B04"B{{NVWW7// ???''ӈ$I޼y3>>Qʹs熄MLLb1b޾}ZZZ*DRPPp՘DI oõ +W ( jii?D^hBQTuuuXXX $ѣ^ RMMMꪪ 0٩gnnP4- wѧOdϟjjj hG4hM?޽{8qbSSX-ـi Vhhh jooolllkkA|||rr2N֎hDpL&khh|2EQLeff D%%%^^^޽0TUU?yL`=kjj?~|-Sggg=5D{f9 ;w!<pqEݺu+++ OL(h! ,uT͛R!slnnNKK 0sHTWW][QMMMLL͛7Z:b~^^SRRtDRZZ啘S]]}ʕvD՚ϟ3 NOOJ0ݾ}޽ىPTTٳBLRlw42 -4M}6&&Lz$6$n߾ikkxɋ/:::C3EEGG?~8///==HI?+I***#F333dtvv~'O166|%KRޞ%I255wsioo766&۶" @b8p*C(J +++aA$''+))MII׏*djj g k9݁hL9ӧOp@}}}FaTѯ_?''' +99/ ``hh8}NKKKغJPHDFF ܖ(QSSJ4#ݻwzWSS#4===]]/^XXXpE 5C|tvv?(ǏR4777--mqqqv}UUU+V.~<%.>y򤼼o'.%&&w„ JKKV^B/JJJ6mx0UUUK?]{LS?K +( fL*ÍtqjqY6c%5%KtLp.LT  bݠ\z NK=9{}}s?sP(;( Rt8[n%vrrrlll||xF$RV!'?4LL&SRutt|z~׮]̌4===VH$A^xt:鬭ټy3njooLJJƱ 2@ `0 ,ݻAbJr8l6^#4:`0=zL&{Q{.8J$Yt:]b"H((Lx#lllvZ$YzX,)(t7o2;wf`0n:X\\\ dl7oF"7r\]bT* FOOh4x)pWz]~۷Y,۷ T;w0 J̩K.x<!]|90LTB'd655 Aw&xp]/~‚c0/_0ܹp޻wOV߼y3fee ΫWj4ł5kJJJ0 Bϟ===PhÆ @VP 0 }r-l?#x<]]] (~%$$444kssG ݻw .qI8vŋ|>(D"r8k׮/ 3uLJ_}lQpF^.j|GQ411}A1CCCWZǎJLsrr֮]qFɞLfٜN~D8 7nBN> 6544L&344T]]!@ jH$t۶mKII^6778NB!pž~:҃OB`vywqu BReffTTTT*Br\'O` *=Rd\(z333/R~~-[*6]SSj9vڠ611ÇUUUCCC}P([11Lvkjjd2 E__T*jR_ONNF* Tvf'|@foAO: BCC8޻wL&U`)lV++++=jllH$`vԩ@ .4ɓ' @ @Q744 7::ZYYYPPE+77WզTUU}%Az lxaAAAEEťK*++ |ᇊ rW8zhwwwAA0 ۾};ñZ0~fY۷iii`WD~嗚Z- )h{E`*++:&--mZ6//oӦM2``fZ{{{}@ SRRLaؕ+WjjjD"ў={L& H]]ݮ]@ݴiH$)(-[h4LVVV q\rM(VWW'%%mUSB'<[roߞ (feeٳ7 8Ή'._ -A5MNN|jooOII)--o۶-;;l\.Www\.ݿtҡCFGGҪLL&Fر7 tvv(m6Z ΐYYYx:::r98tiiiL&Y__uV@ׯ_ wܩT*322xl&I.u#U+!! \ÇpF)#L$4D;]]]Qze~4FgwwမZ888Hj~~ T?sYz."ԒA OY/<9!,4w("N"OJ"؅gbuZdN¹XM&|7$4yJ 1r(3f45;huųâ7i(:O`̰,ɋ'2f(E'J#Y% 9yq&#%Ebhdt D"haQ:%ѐZ$ڑ({%,^ցRNp[x&94pBD ,,egad3h%dkcHa@I6nEU!PGv4 i"OU؅hqAR F3!y%P)~|A<, <nD"NOT8MOO   qi#60 BH1 Jbr !z~jj`0D Og+SJ420On VX8?kZY,RߏaHą4 f'%[xU? %Je8P*"]`KȡAV45侲Tdaa@2;;+ x<2fرCծZt졡!ܩ$cZ]RR:bw\LNN9sL`={d?ֺ\.]. "X811p8Ri0d2f9##crrjl\1))iffVQ&4lbjL466* A6lPZZd2O>bl7|VVV믿JwFcaaȈbّHv4KT/񍏏{<s&_X,dR)Z[[QdL&(r&&&5 ^j B# ~8_@bA,S%}!\ZZ # U*8<ϗv ^P(D"p82l͚5($%%9NX k˫۩/_cǎo655$33X~nnnjj*{WdJOO)Gƶq $7r\.#v8"`D峳%b$55喖B!$V+G@+VOTLPp^E[[[7or322FGG\ffy~~^$Fy9!9%SN6M$I$JnZJ$MOO㯫ʱ8>2R`X,*ÉĢ<_Ș}L`~ %-t ˰ޝx lp1hVK#v^\x܏hr(UP|`aM!8OL00~U_'gN?sQp<)}ړfS/cN0~7Ki^4 UыAqr馩GeՒ$W=[/aq *1U endstream endobj 261 0 obj << /Type /Annot /Subtype /Link /A 262 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 147.6195 260.2500 232.3695 ] >> endobj 262 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure1.png) >> endobj 263 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 264 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 263 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 25625>> stream xgXG8KT+X@anl!1${IbhTĠ1"Q HGsv>~z^3wgݝ%)"Ii ` \ 0<X~eUF`U[J.\Aaάc9#%ߍ-[-*rGPzA`2z$8!,)YάR`|Y=3GtFFV7g8Kvt8>9l/Ht掄6+LS{WR񛏄5JpAʹ+kG$"hUxF ʾP~.\Z ix'9+mo7V!j+ .~DKJJD"(i8x`XXXDDMӭ6l`(Rɓ4MRQ\\! iũѣG۶m>|ɑdeeeR:##C&UTTTWW3%4M766|Q(mmmwmjjJHHx(߆ \oqÆ >ăxrXX^^_ bxxX9">\$ӧLOO‚ #F3&66v0rbbbssssssiii>}nݺ8rM6)))M<9(((//ٳgFFFb_SSɓʧN"Is̹vZ~~~ttǛݧOq!f͚}o>ٳgoذaӦM_nnn.**7nĉMLL/_ԔW^^^[[h"eڴi6m3g(Yz)S6m3pƺUZ0' ,kY1>>bssAܾ}{dd$… fffՌmmm2VU$eeeH{cǎϙ3'}AlllA/N:uӦMg%k֬qttܴi9DL988())577ڵqGqnnnNf--GSЇ%O5 1$ɖ]vȥ WN+ 8N)vwn)(~rAnT0J8uHgg ѵhooYS;뜐z7It0rbSb 貪NJ#\e\>rW@TH!>gV<|"wL\Bq=NEz@k~5"$@E@(ݻ髯B>=vxKAy{ w{_J`<"ME֛u.VH\{섞/2ExvmTVVڶkkkkkksi J, ZJ SbUUPP۷?^PP@Dyys{{;1pWQdJnwwr\q9\~Y(jQQmnݺܜ~lVWWWSS#*ED$OLH 48ޏ ~˅ ɚxJJJrsspsn޼y&Lx۷BBB֭[a܂gqg4 OVQ1kcU؈Ӓ$vU(;wY~ר(kkkG*++ gϞ.e+ٳrﰦ6Ӳa1 8x@Dj#ɓƍĕ.L;v֭["(88y---B%55U*Vs9{%/ J Bjx21]|WX ܹsT__ƍSLuppرcG@@@KKː!Cbbb̼~||>>ßsEGGXK<+B^<؂3'y,**(2r)rEP FiiiioaaW`Zm@O?577_|[n7׶EEEb8""o߾JJJ۷o_|]]],X~PPPЎ;hgp',@Dggqg`` Hd``@tiOOk׮;RCCcYYY?~9r!CF˷\r…̸K[ZZ$dT Q UEE۷'O}~uP ƍfeeyxxhii9;;Ϛ5k޼yVZ|5k֭[k׮K۷OEE͛7+W\)G8 >|6m ŷnݒA \yeff]6**jݺuCMHH4iePPEQ9OصkFTTm6lK/^|͝;wwƍ#GdڿL<͛7ϟ?:8::}/11qVVV.]ڵkɓ'B? ^#ɵeee***r}yAKKeܹ3g-^ -???##tϟܹ k׮|ٳg)S8::^xŋzJHHqF||P(433CI֭[߿ogg6i$CCCggg3f<~BIIi̙G=tЍ7 4vE9::z |O}믿f͚ ,++xW_}zj0gDAee>}yH$+++| য়~>|*,,TVV Y(ׯ_o߾̱O>b 7:u Fc%_ 6eV;;;===oݺCIR7;_555.\2H-dFGGggg]'lmmE"EQLJJz̙3w JKK۹sguu5EQgϞ(J*^xtMӝvyŋ冞>\ZZdcǎGDDl߾}ϟ?okkdrEY;.{bh͞3f̐{C?W- @__ԩ3=f̄ge `eee###>{3g߿Ĉ:::?f~~_￟8qe4My&..EWW˗/WUUݻW^rC@wޓܣ=^x'K@={v~~#G-[FQիW>߿~,YJtSSSMMP($I(.. twtt_~#Gwٳgp=YXWQe``iaa1dHdaaFQ})Aܹs'BaI'* *S277ge1W\QVV=z'ŅKId>|MӃ>qpΓ'O6o޼ut###SSӺ\r***GٴiŋwtnƃT__OA޽yL߼y6} 4㖖/BOO/00.111..ڵk K.%IrڴiW^MMM_ yM\\ܲeˤRiJJJJJʕ+>}Y2L 8ήt_$0sGs,O>#q a#<]X, ~𡃃… AW8p Azzz:::***C 9s@ ѳ hjj \g4dnn4&xj[[`SN]zuHHȝ;w?>ڿoAX[[XZZd?Z"رǏ͐$yٻwM:ѣG^۷/yyy/_Ojjj}qܹsooׯ_S|m6uuuǎ 1mmw?o߾}}L''/BEE...&&&C  L p|27$I^ʪw`u4[0J|Ǐk֬isȐ!/4=a„qƁ͎Fe˖!C̘1c„ wޕdyX4cbbbbbRRR"##mmm) /Żw={j*}Y[Zqq1xl :o<UV!NWWBGGӧ˖-311ikk3f M999{```cce[[X,޽{v={j%  Z Ǜ7%''4tǏO< SrwVVVT0=PRRXzG}}OVVI#F$YZZ IR&8q" ˗1%#I|T 2e & Xgll Mr a`ҤI9bxĈ$I:99޽~qP(ܳgϮ]~嗈+++OP8{쒒;;;V|L-ZTSSsȑ 6 'O0`ʦM벳˕H477_@&IR]],D$I4غ$>}M:uϞ=۶md4ijj7a„ӧ͜9uGWd2IC^$''GFF$YRRdzgϖ.]LQY@@s缽CBBTUUmfffW_ٳ'))}ɒ%QQQeee`ӧOgggK$t$4Id$ y eee9!EQOCpp0xAȽ{ pyRw%&&п5//<(cرAСC(xŅ>>> ֛7oRSS)***oKdeeQj*/]]]/_,H1 7Shّll7֭[BQ۷2Ο?Y[[˅9!(ƍGӦM377’GGG___kHQԥK(z왽s/_dggw]vHҲ20%EyEQ`dmm파 :;;O> <,YBQD"9uT{{;*TD"@vڀR{^!޽{Eeee=zhܸq6lx왓ÇwOOI&EGGU)S\xۛ!]VG;y7%m?閖և Ǐ?#x~ uuuHP vPYY"&MeggW^eff}7&MbB8v쇩ZUUyff>}:1lذW:99&7o޸Ӂ'NP% O:u[Aϟ?򊎎Gcd%K$ikk{}f_ L,o޼͛4Mo߾_~0bciicǒh ⴵ innniGM> ,pss[vٳ_~ $bɞ RRRꫯ.\%s]xҀΞ=3sm۶-Y4ĥ2Sֲ2߿=G,fY^^~ᶶ6DiӦZ@n:s ѱbŊcǎݹs+;aLLѣG޽ L'iŋǏ?{,0w=~J4 zȾ}̞ gggX,J$ @}+_ݨ(~WXrJеo@׮][RR nEH1b͛7KwE! W]p766_ _?...Ν;uuu !3\O$oܸ L&ӧϡCnذ!''gJOO/;;[*ڵk֭BÇZZZ6$$;w?#G433;px! eee&3F<0%)**JHH㇟_{{X,i:77wΝ/^i?ȏ{A̙3಴͛~~~%%%hUU͛7`uuuMMM'N([/Ahiiفq6@<[Bu}!(:::,, Ι3͛2??4M3طoD"d2/4MWTT,^_Ǝ &r+W7o^~~>I?޽P SSP*]2]UUu޼y9rή( w CD(Vxb%7o޻w@D"]5k$''SuU7F]]c@ջw'Eͼ'N~.ɓ+Vttt>|8!!aܸq)))aaa_74M/)) {nIKKɓ';gnnݻA',//?uԥK@#}ե2>>>ϟg߿/@aHHH8oÆ sqqaWZ[[l߾W^ .;v޽{mfeehoݺU__Ot~~>G9`p>}tp۷o0Rt-򒒒@CLII8q+++fMǍ,:;;4tĉV ^ ඁ($l9r ˟<())eѣG>>Ǐ;;;ܹ5i$fbCFA@&1(*$$СCE^t)22i~~~gpYPP$`5[]]DǏWNOOwppػw뛛R)$IC>D4t=\ L ߿ϼr]UUWWW? 2###AHMM ӧiHHt-g<_Fwvv۷LRԜ;;;XgU*-----u BfX=z/ )jnnXuuuA۷… T d0ߵkWee%`~7n0/ W߾} ػw/xBstE,>>|MN֭[4 G\ ~%4GABV92-xIfh߾}Ah"6lؐIQTFFP6€Ixeee'sss9].Û7owȮ[!33>>mmmAڒ$ fee:tH[[{_5A2ڵk+<4T\\lffcBBB~iӦ}G}(6)Gq!'W`߾} ʕ+nnnQQQb(### FyL7G.(SgVoVWW9rm33kGGFLBeÇ߿Y ֭?ϝgΜL£GrrrFP(MԿ(Z|Y@UpEݻ/^ˆt_.MQǏmllx 3-[l2֜ͻJJJ+W_=zpBCf\UU#33333311QYY2={fBkkkhh"c5e}FUgF\(N#ɦLd``wCYgd! _oaa1|p0"73Yܟ-r#r{"ƾSDO~R8&,eС`nhmmDѣG/^f͚777q%2U666`%QѬma,\p>|ѝꉌ \ng D))) f<}hH9~J`vteB5b?.MW+; s9h[ڮ]]svշhPbC\hhhHQ/L&kkk---577WUU8š[v, NA4#>'fΜVTTosss띝,5_*X?<Oϟ?m4 333{eff <āi|" ?;,nDBzʕ¢W^Çg6Չ.h`9SSa 7.=ց055u̘1W%VVVjjjMMMPxׯˍ l5 |Qsv֭߃򂂂Z[[ۈf~a˖-s5(<\[qqxb^ma}ꫯMMMA1ؠC Gb]'oHCX:6Vq$,U:[W fҤIyyyE++!C$9x`NKT D"c F[,Bj+m"h(iV*\-\5< JZʙ专!0zs"WE eprwx!km6"]n$>\IIIOOQ fXCVws\U?:nŴtgU#W!>PdV~حi9| |)"~ɃƜ#]p9$/p3N'FrIL23=dZ^)ŠVBT<4rEH9T&s<x". K-!'_p _qaUk\rϟn?e @V)H[;irH+*b .uBaĴ-n<rVq49":-E`U qUbO~9gD{62pg˪""x x.Y>}脴fېreΚ0 n9kBIG31~ L!2h,rilJG B> BTO|7* ,wcۅ( >n9II\Tb5_'&H`Q$4ݘcꌛ~t$“(, 'j|0+ bul +NXBfnAT"<AK r\\09 .B\c2?*ELҿ(h!]j($_aё5]6zb$33q!P~777KKK :;;Aյkzh"plٲ;w󶶶۷_2 b8f\\۷ow.o~ASSS˗۶m7o>vX>}9v؅ @չsN3ԄK$̙3`Y 4My)'JOOy K.}Մ{TIRRRAA}B04"B{{NVWW7// ???''ӈ$I޼y3>>Qʹs熄MLLb1b޾}ZZZ*DRPPp՘DI oõ +W ( jii?D^hBQTuuuXXX $ѣ^ RMMMꪪ 0٩gnnP4- wѧOdϟjjj hG4hM?޽{8qbSSX-ـi Vhhh jooolllkkA|||rr2N֎hDpL&khh|2EQLeff D%%%^^^޽0TUU?yL`=kjj?~|-Sggg=5D{f9 ;w!<pqEݺu+++ OL(h! ,uT͛R!slnnNKK 0sHTWW][QMMMLL͛7Z:b~^^SRRtDRZZ啘S]]}ʕvD՚ϟ3 NOOJ0ݾ}޽ىPTTٳBLRlw42 -4M}6&&Lz$6$n߾ikkxɋ/:::C3EEGG?~8///==HI?+I***#F333dtvv~'O166|%KRޞ%I255wsioo766&۶" @b8p*C(J +++aA$''+))MII׏*djj g k9݁hL9ӧOp@}}}FaTѯ_?''' +99/ ``hh8}NKKKغJPHDFF ܖ(QSSJ4#ݻwzWSS#4===]]/^XXXpE 5C|tvv?(ǏR4777--mqqqv}UUU+V.~<%.>y򤼼o'.%&&w„ JKKV^B/JJJ6mx0UUUK?]{LS?K +( fL*ÍtqjqY6c%5%KtLp.LT  bݠ\z NK=9{}}s?sP(;( Rt8[n%vrrrlll||xF$RV!'?4LL&SRutt|z~׮]̌4===VH$A^xt:鬭ټy3njooLJJƱ 2@ `0 ,ݻAbJr8l6^#4:`0=zL&{Q{.8J$Yt:]b"H((Lx#lllvZ$YzX,)(t7o2;wf`0n:X\\\ dl7oF"7r\]bT* FOOh4x)pWz]~۷Y,۷ T;w0 J̩K.x<!]|90LTB'd655 Aw&xp]/~‚c0/_0ܹp޻wOV߼y3fee ΫWj4ł5kJJJ0 Bϟ===PhÆ @VP 0 }r-l?#x<]]] (~%$$444kssG ݻw .qI8vŋ|>(D"r8k׮/ 3uLJ_}lQpF^.j|GQ411}A1CCCWZǎJLsrr֮]qFɞLfٜN~D8 7nBN> 6544L&344T]]!@ jH$t۶mKII^6778NB!pž~:҃OB`vywqu BReffTTTT*Br\'O` *=Rd\(z333/R~~-[*6]SSj9vڠ611ÇUUUCCC}P([11Lvkjjd2 E__T*jR_ONNF* Tvf'|@foAO: BCC8޻wL&U`)lV++++=jllH$`vԩ@ .4ɓ' @ @Q744 7::ZYYYPPE+77WզTUU}%Az lxaAAAEEťK*++ |ᇊ rW8zhwwwAA0 ۾};ñZ0~fY۷iii`WD~嗚Z- )h{E`*++:&--mZ6//oӦM2``fZ{{{}@ SRRLaؕ+WjjjD"ў={L& H]]ݮ]@ݴiH$)(-[h4LVVV q\rM(VWW'%%mUSB'<[roߞ (feeٳ7 8Ή'._ -A5MNN|jooOII)--o۶-;;l\.Www\.ݿtҡCFGGҪLL&Fر7 tvv(m6Z ΐYYYx:::r98tiiiL&Y__uV@ׯ_ wܩT*322xl&I.u#U+!! \ÇpF)#L$4D;]]]Qze~4FgwwမZ888Hj~~ T?sYz."ԒA OY/<9!,4w("N"OJ"؅gbuZdN¹XM&|7$4yJ 1r(3f45;huųâ7i(:O`̰,ɋ'2f(E'J#Y% 9yq&#%Ebhdt D"haQ:%ѐZ$ڑ({%,^ցRNp[x&94pBD ,,egad3h%dkcHa@I6nEU!PGv4 i"OU؅hqAR F3!y%P)~|A<, <nD"NOT8MOO   qi#60 BH1 Jbr !z~jj`0D Og+SJ420On VX8?kZY,RߏaHą4 f'%[xU? %Je8P*"]`KȡAV45侲Tdaa@2;;+ x<2fرCծZt졡!ܩ$cZ]RR:bw\LNN9sL`={d?ֺ\.]. "X811p8Ri0d2f9##crrjl\1))iffVQ&4lbjL466* A6lPZZd2O>bl7|VVV믿JwFcaaȈbّHv4KT/񍏏{<s&_X,dR)Z[[QdL&(r&&&5 ^j B# ~8_@bA,S%}!\ZZ # U*8<ϗv ^P(D"p82l͚5($%%9NX k˫۩/_cǎo655$33X~nnnjj*{WdJOO)Gƶq $7r\.#v8"`D峳%b$55喖B!$V+G@+VOTLPp^E[[[7or322FGG\ffy~~^$Fy9!9%SN6M$I$JnZJ$MOO㯫ʱ8>2R`X,*ÉĢ<_Ș}L`~ %-t ˰ޝx lp1hVK#v^\x܏hr(UP|`aM!8OL00~U_'gN?sQp<)}ړfS/cN0~7Ki^4 UыAqr馩GeՒ$W=[/aq *1U endstream endobj 265 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 266 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 265 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 25625>> stream xgXG8KT+X@anl!1${IbhTĠ1"Q HGsv>~z^3wgݝ%)"Ii ` \ 0<X~eUF`U[J.\Aaάc9#%ߍ-[-*rGPzA`2z$8!,)YάR`|Y=3GtFFV7g8Kvt8>9l/Ht掄6+LS{WR񛏄5JpAʹ+kG$"hUxF ʾP~.\Z ix'9+mo7V!j+ .~DKJJD"(i8x`XXXDDMӭ6l`(Rɓ4MRQ\\! iũѣG۶m>|ɑdeeeR:##C&UTTTWW3%4M766|Q(mmmwmjjJHHx(߆ \oqÆ >ăxrXX^^_ bxxX9">\$ӧLOO‚ #F3&66v0rbbbssssssiii>}nݺ8rM6)))M<9(((//ٳgFFFb_SSɓʧN"Is̹vZ~~~ttǛݧOq!f͚}o>ٳgoذaӦM_nnn.**7nĉMLL/_ԔW^^^[[h"eڴi6m3g(Yz)S6m3pƺUZ0' ,kY1>>bssAܾ}{dd$… fffՌmmm2VU$eeeH{cǎϙ3'}AlllA/N:uӦMg%k֬qttܴi9DL988())577ڵqGqnnnNf--GSЇ%O5 1$ɖ]vȥ WN+ 8N)vwn)(~rAnT0J8uHgg ѵhooYS;뜐z7It0rbSb 貪NJ#\e\>rW@TH!>gV<|"wL\Bq=NEz@k~5"$@E@(ݻ髯B>=vxKAy{ w{_J`<"ME֛u.VH\{섞/2ExvmTVVڶkkkkkksi J, ZJ SbUUPP۷?^PP@Dyys{{;1pWQdJnwwr\q9\~Y(jQQmnݺܜ~lVWWWSS#*ED$OLH 48ޏ ~˅ ɚxJJJrsspsn޼y&Lx۷BBB֭[a܂gqg4 OVQ1kcU؈Ӓ$vU(;wY~ר(kkkG*++ gϞ.e+ٳrﰦ6Ӳa1 8x@Dj#ɓƍĕ.L;v֭["(88y---B%55U*Vs9{%/ J Bjx21]|WX ܹsT__ƍSLuppرcG@@@KKː!Cbbb̼~||>>ßsEGGXK<+B^<؂3'y,**(2r)rEP FiiiioaaW`Zm@O?577_|[n7׶EEEb8""o߾JJJ۷o_|]]],X~PPPЎ;hgp',@Dggqg`` Hd``@tiOOk׮;RCCcYYY?~9r!CF˷\r…̸K[ZZ$dT Q UEE۷'O}~uP ƍfeeyxxhii9;;Ϛ5k޼yVZ|5k֭[k׮K۷OEE͛7+W\)G8 >|6m ŷnݒA \yeff]6**jݺuCMHH4iePPEQ9OصkFTTm6lK/^|͝;wwƍ#GdڿL<͛7ϟ?:8::}/11qVVV.]ڵkɓ'B? ^#ɵeee***r}yAKKeܹ3g-^ -???##tϟܹ k׮|ٳg)S8::^xŋzJHHqF||P(433CI֭[߿ogg6i$CCCggg3f<~BIIi̙G=tЍ7 4vE9::z |O}믿f͚ ,++xW_}zj0gDAee>}yH$+++| য়~>|*,,TVV Y(ׯ_o߾̱O>b 7:u Fc%_ 6eV;;;===oݺCIR7;_555.\2H-dFGGggg]'lmmE"EQLJJz̙3w JKK۹sguu5EQgϞ(J*^xtMӝvyŋ冞>\ZZdcǎGDDl߾}ϟ?okkdrEY;.{bh͞3f̐{C?W- @__ԩ3=f̄ge `eee###>{3g߿Ĉ:::?f~~_￟8qe4My&..EWW˗/WUUݻW^rC@wޓܣ=^x'K@={v~~#G-[FQիW>߿~,YJtSSSMMP($I(.. twtt_~#Gwٳgp=YXWQe``iaa1dHdaaFQ})Aܹs'BaI'* *S277ge1W\QVV=z'ŅKId>|MӃ>qpΓ'O6o޼ut###SSӺ\r***GٴiŋwtnƃT__OA޽yL߼y6} 4㖖/BOO/00.111..ڵk K.%IrڴiW^MMM_ yM\\ܲeˤRiJJJJJʕ+>}Y2L 8ήt_$0sGs,O>#q a#<]X, ~𡃃… AW8p Azzz:::***C 9s@ ѳ hjj \g4dnn4&xj[[`SN]zuHHȝ;w?>ڿoAX[[XZZd?Z"رǏ͐$yٻwM:ѣG^۷/yyy/_Ojjj}qܹsooׯ_S|m6uuuǎ 1mmw?o߾}}L''/BEE...&&&C  L p|27$I^ʪw`u4[0J|Ǐk֬isȐ!/4=a„qƁ͎Fe˖!C̘1c„ wޕdyX4cbbbbbRRR"##mmm) /Żw={j*}Y[Zqq1xl :o<UV!NWWBGGӧ˖-311ikk3f M999{```cce[[X,޽{v={j%  Z Ǜ7%''4tǏO< SrwVVVT0=PRRXzG}}OVVI#F$YZZ IR&8q" ˗1%#I|T 2e & Xgll Mr a`ҤI9bxĈ$I:99޽~qP(ܳgϮ]~嗈+++OP8{쒒;;;V|L-ZTSSsȑ 6 'O0`ʦM벳˕H477_@&IR]],D$I4غ$>}M:uϞ=۶md4ijj7a„ӧ͜9uGWd2IC^$''GFF$YRRdzgϖ.]LQY@@s缽CBBTUUmfffW_ٳ'))}ɒ%QQQeee`ӧOgggK$t$4Id$ y eee9!EQOCpp0xAȽ{ pyRw%&&п5//<(cرAСC(xŅ>>> ֛7oRSS)***oKdeeQj*/]]]/_,H1 7Shّll7֭[BQ۷2Ο?Y[[˅9!(ƍGӦM377’GGG___kHQԥK(z왽s/_dggw]vHҲ20%EyEQ`dmm파 :;;O> <,YBQD"9uT{{;*TD"@vڀR{^!޽{Eeee=zhܸq6lx왓ÇwOOI&EGGU)S\xۛ!]VG;y7%m?閖և Ǐ?#x~ uuuHP vPYY"&MeggW^eff}7&MbB8v쇩ZUUyff>}:1lذW:99&7o޸Ӂ'NP% O:u[Aϟ?򊎎Gcd%K$ikk{}f_ L,o޼͛4Mo߾_~0bciicǒh ⴵ innniGM> ,pss[vٳ_~ $bɞ RRRꫯ.\%s]xҀΞ=3sm۶-Y4ĥ2Sֲ2߿=G,fY^^~ᶶ6DiӦZ@n:s ѱbŊcǎݹs+;aLLѣG޽ L'iŋǏ?{,0w=~J4 zȾ}̞ gggX,J$ @}+_ݨ(~WXrJеo@׮][RR nEH1b͛7KwE! W]p766_ _?...Ν;uuu !3\O$oܸ L&ӧϡCnذ!''gJOO/;;[*ڵk֭BÇZZZ6$$;w?#G433;px! eee&3F<0%)**JHH㇟_{{X,i:77wΝ/^i?ȏ{A̙3಴͛~~~%%%hUU͛7`uuuMMM'N([/Ahiiفq6@<[Bu}!(:::,, Ι3͛2??4M3طoD"d2/4MWTT,^_Ǝ &r+W7o^~~>I?޽P SSP*]2]UUu޼y9rή( w CD(Vxb%7o޻w@D"]5k$''SuU7F]]c@ջw'Eͼ'N~.ɓ+Vttt>|8!!aܸq)))aaa_74M/)) {nIKKɓ';gnnݻA',//?uԥK@#}ե2>>>ϟg߿/@aHHH8oÆ sqqaWZ[[l߾W^ .;v޽{mfeehoݺU__Ot~~>G9`p>}tp۷o0Rt-򒒒@CLII8q+++fMǍ,:;;4tĉV ^ ඁ($l9r ˟<())eѣG>>Ǐ;;;ܹ5i$fbCFA@&1(*$$СCE^t)22i~~~gpYPP$`5[]]DǏWNOOwppػw뛛R)$IC>D4t=\ L ߿ϼr]UUWWW? 2###AHMM ӧiHHt-g<_Fwvv۷LRԜ;;;XgU*-----u BfX=z/ )jnnXuuuA۷… T d0ߵkWee%`~7n0/ W߾} ػw/xBstE,>>|MN֭[4 G\ ~%4GABV92-xIfh߾}Ah"6lؐIQTFFP6€Ixeee'sss9].Û7owȮ[!33>>mmmAڒ$ fee:tH[[{_5A2ڵk+<4T\\lffcBBB~iӦ}G}(6)Gq!'W`߾} ʕ+nnnQQQb(### FyL7G.(SgVoVWW9rm33kGGFLBeÇ߿Y ֭?ϝgΜL£GrrrFP(MԿ(Z|Y@UpEݻ/^ˆt_.MQǏmllx 3-[l2֜ͻJJJ+W_=zpBCf\UU#33333311QYY2={fBkkkhh"c5e}FUgF\(N#ɦLd``wCYgd! _oaa1|p0"73Yܟ-r#r{"ƾSDO~R8&,eС`nhmmDѣG/^f͚777q%2U666`%QѬma,\p>|ѝꉌ \ng D))) f<}hH9~J`vteB5b?.MW+; s9h[ڮ]]svշhPbC\hhhHQ/L&kkk---577WUU8š[v, NA4#>'fΜVTTosss띝,5_*X?<Oϟ?m4 333{eff <āi|" ?;,nDBzʕ¢W^Çg6Չ.h`9SSa 7.=ց055u̘1W%VVVjjjMMMPxׯˍ l5 |Qsv֭߃򂂂Z[[ۈf~a˖-s5(<\[qqxb^ma}ꫯMMMA1ؠC Gb]'oHCX:6Vq$,U:[W fҤIyyyE++!C$9x`NKT D"c F[,Bj+m"h(iV*\-\5< JZʙ专!0zs"WE eprwx!km6"]n$>\IIIOOQ fXCVws\U?:nŴtgU#W!>PdV~حi9| |)"~ɃƜ#]p9$/p3N'FrIL23=dZ^)ŠVBT<4rEH9T&s<x". K-!'_p _qaUk\rϟn?e @V)H[;irH+*b .uBaĴ-n<rVq49":-E`U qUbO~9gD{62pg˪""x x.Y>}脴fېreΚ0 n9kBIG31~ L!2h,rilJG B> BTO|7* ,wcۅ( >n9II\Tb5_'&H`Q$4ݘcꌛ~t$“(, 'j|0+ bul +NXBfnAT"<AK r\\09 .B\c2?*ELҿ(h!]j($_aё5]6zb$33q!P~777KKK :;;Aյkzh"plٲ;w󶶶۷_2 b8f\\۷ow.o~ASSS˗۶m7o>vX>}9v؅ @չsN3ԄK$̙3`Y 4My)'JOOy K.}Մ{TIRRRAA}B04"B{{NVWW7// ???''ӈ$I޼y3>>Qʹs熄MLLb1b޾}ZZZ*DRPPp՘DI oõ +W ( jii?D^hBQTuuuXXX $ѣ^ RMMMꪪ 0٩gnnP4- wѧOdϟjjj hG4hM?޽{8qbSSX-ـi Vhhh jooolllkkA|||rr2N֎hDpL&khh|2EQLeff D%%%^^^޽0TUU?yL`=kjj?~|-Sggg=5D{f9 ;w!<pqEݺu+++ OL(h! ,uT͛R!slnnNKK 0sHTWW][QMMMLL͛7Z:b~^^SRRtDRZZ啘S]]}ʕvD՚ϟ3 NOOJ0ݾ}޽ىPTTٳBLRlw42 -4M}6&&Lz$6$n߾ikkxɋ/:::C3EEGG?~8///==HI?+I***#F333dtvv~'O166|%KRޞ%I255wsioo766&۶" @b8p*C(J +++aA$''+))MII׏*djj g k9݁hL9ӧOp@}}}FaTѯ_?''' +99/ ``hh8}NKKKغJPHDFF ܖ(QSSJ4#ݻwzWSS#4===]]/^XXXpE 5C|tvv?(ǏR4777--mqqqv}UUU+V.~<%.>y򤼼o'.%&&w„ JKKV^B/JJJ6mx0UUUK?]{LS?K +( fL*ÍtqjqY6c%5%KtLp.LT  bݠ\z NK=9{}}s?sP(;( Rt8[n%vrrrlll||xF$RV!'?4LL&SRutt|z~׮]̌4===VH$A^xt:鬭ټy3njooLJJƱ 2@ `0 ,ݻAbJr8l6^#4:`0=zL&{Q{.8J$Yt:]b"H((Lx#lllvZ$YzX,)(t7o2;wf`0n:X\\\ dl7oF"7r\]bT* FOOh4x)pWz]~۷Y,۷ T;w0 J̩K.x<!]|90LTB'd655 Aw&xp]/~‚c0/_0ܹp޻wOV߼y3fee ΫWj4ł5kJJJ0 Bϟ===PhÆ @VP 0 }r-l?#x<]]] (~%$$444kssG ݻw .qI8vŋ|>(D"r8k׮/ 3uLJ_}lQpF^.j|GQ411}A1CCCWZǎJLsrr֮]qFɞLfٜN~D8 7nBN> 6544L&344T]]!@ jH$t۶mKII^6778NB!pž~:҃OB`vywqu BReffTTTT*Br\'O` *=Rd\(z333/R~~-[*6]SSj9vڠ611ÇUUUCCC}P([11Lvkjjd2 E__T*jR_ONNF* Tvf'|@foAO: BCC8޻wL&U`)lV++++=jllH$`vԩ@ .4ɓ' @ @Q744 7::ZYYYPPE+77WզTUU}%Az lxaAAAEEťK*++ |ᇊ rW8zhwwwAA0 ۾};ñZ0~fY۷iii`WD~嗚Z- )h{E`*++:&--mZ6//oӦM2``fZ{{{}@ SRRLaؕ+WjjjD"ў={L& H]]ݮ]@ݴiH$)(-[h4LVVV q\rM(VWW'%%mUSB'<[roߞ (feeٳ7 8Ή'._ -A5MNN|jooOII)--o۶-;;l\.Www\.ݿtҡCFGGҪLL&Fر7 tvv(m6Z ΐYYYx:::r98tiiiL&Y__uV@ׯ_ wܩT*322xl&I.u#U+!! \ÇpF)#L$4D;]]]Qze~4FgwwမZ888Hj~~ T?sYz."ԒA OY/<9!,4w("N"OJ"؅gbuZdN¹XM&|7$4yJ 1r(3f45;huųâ7i(:O`̰,ɋ'2f(E'J#Y% 9yq&#%Ebhdt D"haQ:%ѐZ$ڑ({%,^ցRNp[x&94pBD ,,egad3h%dkcHa@I6nEU!PGv4 i"OU؅hqAR F3!y%P)~|A<, <nD"NOT8MOO   qi#60 BH1 Jbr !z~jj`0D Og+SJ420On VX8?kZY,RߏaHą4 f'%[xU? %Je8P*"]`KȡAV45侲Tdaa@2;;+ x<2fرCծZt졡!ܩ$cZ]RR:bw\LNN9sL`={d?ֺ\.]. "X811p8Ri0d2f9##crrjl\1))iffVQ&4lbjL466* A6lPZZd2O>bl7|VVV믿JwFcaaȈbّHv4KT/񍏏{<s&_X,dR)Z[[QdL&(r&&&5 ^j B# ~8_@bA,S%}!\ZZ # U*8<ϗv ^P(D"p82l͚5($%%9NX k˫۩/_cǎo655$33X~nnnjj*{WdJOO)Gƶq $7r\.#v8"`D峳%b$55喖B!$V+G@+VOTLPp^E[[[7or322FGG\ffy~~^$Fy9!9%SN6M$I$JnZJ$MOO㯫ʱ8>2R`X,*ÉĢ<_Ș}L`~ %-t ˰ޝx lp1hVK#v^\x܏hr(UP|`aM!8OL00~U_'gN?sQp<)}ړfS/cN0~7Ki^4 UыAqr馩GeՒ$W=[/aq *1U endstream endobj 267 0 obj << /Type /Page /Parent 3 0 R /Annots [ 269 0 R 273 0 R 277 0 R 281 0 R 285 0 R 289 0 R ] /Contents 268 0 R >> endobj 268 0 obj << /Length 13349 >> stream 0.271 0.267 0.267 rg q 15.000 75.778 577.500 701.222 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(groups showed similar numbers of reinforcements earned across sessions. In contrast, as in the PR-alone training phase \(see )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(above\), the BAC Tg mice earned significantly fewer reinforcers than WT controls during the PR component \(Genotype main )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(effect: F\(1,14\) = 25.0, )] TJ ET BT 122.453 743.667 Td /F5 9.8 Tf [(p < 0.001\). )] TJ ET BT 172.052 743.667 Td /F1 9.8 Tf [(Both groups earned fewer reinforcers in the PR phase as testing progressed, independently of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(genotype \(Session main effect: F\(23,322\) = 3.29, )] TJ ET BT 240.594 731.762 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 286.945 731.762 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 460.599 555.000 261.282 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 721.881 m 581.250 721.881 l 581.250 721.131 l 26.250 721.131 l f 26.250 460.599 m 581.250 460.599 l 581.250 461.349 l 26.250 461.349 l f q 225.000 0 0 169.500 35.250 542.631 cm /I12 Do Q q 35.250 471.849 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 527.107 Td /F4 9.8 Tf [(Fig. 2: Summarized data from mixed FR5/PR training across sessions in Experiment 1A \(left panels\) and )] TJ ET BT 35.250 515.202 Td /F4 9.8 Tf [(Experiment 1B \(right panels\))] TJ ET BT 35.250 495.832 Td /F1 9.8 Tf [(Data from the 10 min FR5 training period are presented in the upper panels, while data from the 80 min PR training period )] TJ ET BT 35.250 482.096 Td /F1 9.8 Tf [(are presented in the lower panels.)] TJ ET Q BT 26.250 443.575 Td /F1 9.8 Tf [(Finally, to break down these overall difference by the specific response requirements, response rates were calculated per )] TJ ET BT 26.250 431.670 Td /F1 9.8 Tf [(reinforcer earned within each session, with these data then collapsed across training sessions for each mouse. In Experiment )] TJ ET BT 26.250 419.766 Td /F1 9.8 Tf [(1A, these response rate data were only collected during the final 8 sessions of FR5/PR training and are presented in the left )] TJ ET BT 26.250 407.861 Td /F1 9.8 Tf [(panels ofFigure 3 below, with data from the FR5 component in the upper panel and from the PR component in the lower panel. )] TJ ET BT 26.250 395.956 Td /F1 9.8 Tf [(Since not all animals earned the same number of total reinforcers in each phase, these data are truncated at the point where )] TJ ET BT 26.250 384.051 Td /F1 9.8 Tf [(most mice were still contributing to the dataset, at 30 reinforcers for the FR5 phase and 12 reinforcers for the PR phase. No )] TJ ET BT 26.250 372.147 Td /F1 9.8 Tf [(differences were apparent in the rate of responding per reinforcer in the FR5 phase, with all animals working at a comparable )] TJ ET BT 26.250 360.242 Td /F1 9.8 Tf [(level. Both groups responded more slowly as session time elapsed independently of genotype \(Session main effect: F\(39,541\) = )] TJ ET BT 26.250 348.337 Td /F1 9.8 Tf [(8.40, )] TJ ET BT 50.645 348.337 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 96.996 348.337 Td /F1 9.8 Tf [(\). Similarly, early in the PR component where the level of effort required per reinforcer was low, both groups of )] TJ ET BT 26.250 336.432 Td /F1 9.8 Tf [(mice appeared comparable \(Figure 3, lower left panel\). However, as the level of effort increased, the BAC Tg mice responded )] TJ ET BT 26.250 324.528 Td /F1 9.8 Tf [(less than did WT controls, with this effect recorded starting at the ratio 50, although the differences missed significance at the )] TJ ET BT 26.250 312.623 Td /F1 9.8 Tf [(ratio 100 \(Response Ratio main effect and Response Ratio x Genotype interaction: smaller F\(10,137\) = 2.15, )] TJ ET BT 498.530 312.623 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 541.625 312.623 Td /F1 9.8 Tf [( simple )] TJ ET BT 26.250 300.718 Td /F1 9.8 Tf [(main effects: smallest F\(1,137\) = 3.98, all )] TJ ET BT 207.512 300.718 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 247.897 300.718 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 75.778 555.000 215.060 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 290.837 m 581.250 290.837 l 581.250 290.087 l 26.250 290.087 l f q 225.000 0 0 169.500 35.250 111.587 cm /I14 Do Q q 35.250 75.778 537.000 29.810 re W n Q Q q 15.000 75.778 577.500 701.222 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(groups showed similar numbers of reinforcements earned across sessions. In contrast, as in the PR-alone training phase \(see )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(above\), the BAC Tg mice earned significantly fewer reinforcers than WT controls during the PR component \(Genotype main )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(effect: F\(1,14\) = 25.0, )] TJ ET BT 122.453 743.667 Td /F5 9.8 Tf [(p < 0.001\). )] TJ ET BT 172.052 743.667 Td /F1 9.8 Tf [(Both groups earned fewer reinforcers in the PR phase as testing progressed, independently of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(genotype \(Session main effect: F\(23,322\) = 3.29, )] TJ ET BT 240.594 731.762 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 286.945 731.762 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 460.599 555.000 261.282 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 721.881 m 581.250 721.881 l 581.250 721.131 l 26.250 721.131 l f 26.250 460.599 m 581.250 460.599 l 581.250 461.349 l 26.250 461.349 l f q 225.000 0 0 169.500 35.250 542.631 cm /I16 Do Q q 35.250 471.849 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 527.107 Td /F4 9.8 Tf [(Fig. 2: Summarized data from mixed FR5/PR training across sessions in Experiment 1A \(left panels\) and )] TJ ET BT 35.250 515.202 Td /F4 9.8 Tf [(Experiment 1B \(right panels\))] TJ ET BT 35.250 495.832 Td /F1 9.8 Tf [(Data from the 10 min FR5 training period are presented in the upper panels, while data from the 80 min PR training period )] TJ ET BT 35.250 482.096 Td /F1 9.8 Tf [(are presented in the lower panels.)] TJ ET Q BT 26.250 443.575 Td /F1 9.8 Tf [(Finally, to break down these overall difference by the specific response requirements, response rates were calculated per )] TJ ET BT 26.250 431.670 Td /F1 9.8 Tf [(reinforcer earned within each session, with these data then collapsed across training sessions for each mouse. In Experiment )] TJ ET BT 26.250 419.766 Td /F1 9.8 Tf [(1A, these response rate data were only collected during the final 8 sessions of FR5/PR training and are presented in the left )] TJ ET BT 26.250 407.861 Td /F1 9.8 Tf [(panels ofFigure 3 below, with data from the FR5 component in the upper panel and from the PR component in the lower panel. )] TJ ET BT 26.250 395.956 Td /F1 9.8 Tf [(Since not all animals earned the same number of total reinforcers in each phase, these data are truncated at the point where )] TJ ET BT 26.250 384.051 Td /F1 9.8 Tf [(most mice were still contributing to the dataset, at 30 reinforcers for the FR5 phase and 12 reinforcers for the PR phase. No )] TJ ET BT 26.250 372.147 Td /F1 9.8 Tf [(differences were apparent in the rate of responding per reinforcer in the FR5 phase, with all animals working at a comparable )] TJ ET BT 26.250 360.242 Td /F1 9.8 Tf [(level. Both groups responded more slowly as session time elapsed independently of genotype \(Session main effect: F\(39,541\) = )] TJ ET BT 26.250 348.337 Td /F1 9.8 Tf [(8.40, )] TJ ET BT 50.645 348.337 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 96.996 348.337 Td /F1 9.8 Tf [(\). Similarly, early in the PR component where the level of effort required per reinforcer was low, both groups of )] TJ ET BT 26.250 336.432 Td /F1 9.8 Tf [(mice appeared comparable \(Figure 3, lower left panel\). However, as the level of effort increased, the BAC Tg mice responded )] TJ ET BT 26.250 324.528 Td /F1 9.8 Tf [(less than did WT controls, with this effect recorded starting at the ratio 50, although the differences missed significance at the )] TJ ET BT 26.250 312.623 Td /F1 9.8 Tf [(ratio 100 \(Response Ratio main effect and Response Ratio x Genotype interaction: smaller F\(10,137\) = 2.15, )] TJ ET BT 498.530 312.623 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 541.625 312.623 Td /F1 9.8 Tf [( simple )] TJ ET BT 26.250 300.718 Td /F1 9.8 Tf [(main effects: smallest F\(1,137\) = 3.98, all )] TJ ET BT 207.512 300.718 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 247.897 300.718 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 75.778 555.000 215.060 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 290.837 m 581.250 290.837 l 581.250 290.087 l 26.250 290.087 l f q 225.000 0 0 169.500 35.250 111.587 cm /I18 Do Q q 35.250 75.778 537.000 29.810 re W n Q Q q 15.000 75.778 577.500 701.222 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(groups showed similar numbers of reinforcements earned across sessions. In contrast, as in the PR-alone training phase \(see )] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(above\), the BAC Tg mice earned significantly fewer reinforcers than WT controls during the PR component \(Genotype main )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(effect: F\(1,14\) = 25.0, )] TJ ET BT 122.453 743.667 Td /F5 9.8 Tf [(p < 0.001\). )] TJ ET BT 172.052 743.667 Td /F1 9.8 Tf [(Both groups earned fewer reinforcers in the PR phase as testing progressed, independently of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(genotype \(Session main effect: F\(23,322\) = 3.29, )] TJ ET BT 240.594 731.762 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 286.945 731.762 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 460.599 555.000 261.282 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 721.881 m 581.250 721.881 l 581.250 721.131 l 26.250 721.131 l f 26.250 460.599 m 581.250 460.599 l 581.250 461.349 l 26.250 461.349 l f q 225.000 0 0 169.500 35.250 542.631 cm /I20 Do Q q 35.250 471.849 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 527.107 Td /F4 9.8 Tf [(Fig. 2: Summarized data from mixed FR5/PR training across sessions in Experiment 1A \(left panels\) and )] TJ ET BT 35.250 515.202 Td /F4 9.8 Tf [(Experiment 1B \(right panels\))] TJ ET BT 35.250 495.832 Td /F1 9.8 Tf [(Data from the 10 min FR5 training period are presented in the upper panels, while data from the 80 min PR training period )] TJ ET BT 35.250 482.096 Td /F1 9.8 Tf [(are presented in the lower panels.)] TJ ET Q BT 26.250 443.575 Td /F1 9.8 Tf [(Finally, to break down these overall difference by the specific response requirements, response rates were calculated per )] TJ ET BT 26.250 431.670 Td /F1 9.8 Tf [(reinforcer earned within each session, with these data then collapsed across training sessions for each mouse. In Experiment )] TJ ET BT 26.250 419.766 Td /F1 9.8 Tf [(1A, these response rate data were only collected during the final 8 sessions of FR5/PR training and are presented in the left )] TJ ET BT 26.250 407.861 Td /F1 9.8 Tf [(panels ofFigure 3 below, with data from the FR5 component in the upper panel and from the PR component in the lower panel. )] TJ ET BT 26.250 395.956 Td /F1 9.8 Tf [(Since not all animals earned the same number of total reinforcers in each phase, these data are truncated at the point where )] TJ ET BT 26.250 384.051 Td /F1 9.8 Tf [(most mice were still contributing to the dataset, at 30 reinforcers for the FR5 phase and 12 reinforcers for the PR phase. No )] TJ ET BT 26.250 372.147 Td /F1 9.8 Tf [(differences were apparent in the rate of responding per reinforcer in the FR5 phase, with all animals working at a comparable )] TJ ET BT 26.250 360.242 Td /F1 9.8 Tf [(level. Both groups responded more slowly as session time elapsed independently of genotype \(Session main effect: F\(39,541\) = )] TJ ET BT 26.250 348.337 Td /F1 9.8 Tf [(8.40, )] TJ ET BT 50.645 348.337 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 96.996 348.337 Td /F1 9.8 Tf [(\). Similarly, early in the PR component where the level of effort required per reinforcer was low, both groups of )] TJ ET BT 26.250 336.432 Td /F1 9.8 Tf [(mice appeared comparable \(Figure 3, lower left panel\). However, as the level of effort increased, the BAC Tg mice responded )] TJ ET BT 26.250 324.528 Td /F1 9.8 Tf [(less than did WT controls, with this effect recorded starting at the ratio 50, although the differences missed significance at the )] TJ ET BT 26.250 312.623 Td /F1 9.8 Tf [(ratio 100 \(Response Ratio main effect and Response Ratio x Genotype interaction: smaller F\(10,137\) = 2.15, )] TJ ET BT 498.530 312.623 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 541.625 312.623 Td /F1 9.8 Tf [( simple )] TJ ET BT 26.250 300.718 Td /F1 9.8 Tf [(main effects: smallest F\(1,137\) = 3.98, all )] TJ ET BT 207.512 300.718 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 247.897 300.718 Td /F1 9.8 Tf [(\).)] TJ ET 0.965 0.965 0.965 rg 26.250 75.778 555.000 215.060 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 290.837 m 581.250 290.837 l 581.250 290.087 l 26.250 290.087 l f q 225.000 0 0 169.500 35.250 111.587 cm /I22 Do Q q 35.250 75.778 537.000 29.810 re W n Q Q q 225.000 0 0 169.500 35.250 542.631 cm /I24 Do Q q 225.000 0 0 169.500 35.250 111.587 cm /I26 Do Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(4)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 269 0 obj << /Type /Annot /Subtype /Link /A 270 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 542.6310 260.2500 712.1310 ] >> endobj 270 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure2.png) >> endobj 271 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 272 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 271 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 46375>> stream xw\:s" h&**6MFcQ+Xb* M@:ʡr?q޻Z{aA  >a s  kiX'O%狓F B@Du8R "&1τ pC? NP50PL!ڊob1)%Ep.(fmrz E#C@; }LGA,RW_`@X* MMM111Z . ϺW^wsݽ{±C^XXFjғ'O^DDDtvvill,..NMME㭸ĉ… @ ۷o &j|ww???Rh`;77 APUUՆ <==;dRzuMM =O`` x<X֧O(gjj* [nv 4'` iii$"f@/_looyNN=H$*--===EEE ?666EEEmmmUUU\.7;;677\^\\\YYp,Y t.\|8q j^mmmcccNNP(c{{;`Ņbᔔ eeeEEE{|>ӦMaaa---eee ;;ʾ⚚WWWWVV>}r X,tϿwׯQ;7m^BCC=z^pA$1I;4rRSS{zzHyZZZ|}}J"""b۶m 'tttݻw֬Yb8++ ׿Y[[T\\immQ\__ۛ';;;7n{nʕ lAAP(<&;;[ UVV655.bܗ/_bK,߽{7!!A,{zzbs'%%b^СC?-mmmffffff0 gff>xXAAPRRRoooKKKUU۷UUU_>iҤիW+((ܹsgƌ׮]p8Ϟ=3008x RRRzqGGիWӧ744ڵK$?>22l̘1Tkk)SD]v>wΝ𦦦//^xӧOv횲rBBBwwwdddyyyuu50233S,ϙ3G(khh\r$001c|}}>|8f̘@ ݻwǏeJJJlllyyP(|w}[^^󛚚>|YYYkk>dggGGGrܾ>]]]l%\A+HT__?}#F0TTT4vXw}6]UU H__ݻw%$$@IHH())illdX޽kmm}Ʌ ,XpB.n>[nuww'''C?{l@0u>,XYYYÇoooWWW3gTTT^|8nܸӧO;888pb=|nϞ=|>?**jӦM0 &&&Ι3SKK#$$#**իbܹsb8..֭[<ŋB˗>v옇 ÷o^hԭ[ VUUZ\\ jޏ?_999ֺAAA$cYӈ:666pN (//t)b^WWp8H$>pĉMLLl윙&..NYYyÆ JJJ ҥKl٢a==ްW^-[̙3g '..޹sd>,@wwwooouuu/CCCCCӧOo޼ѣGՅϞ=_wJKKO8q޼y999ظeeeoo UUխ[{n޼+)))p\. >}fŧO믿nݺA^vv6`>aP(##.zzzA믿JKKњ$\{{ڵk<<< ڲejjj>| Aɓ:33 6zxxxxxfggƊ+o^\\lcc3yd---444P,RCC3iҤuihh˗/=|||-[^QQݻ[[M6YZZ6qƍ1bȑ+W4irYY* 777//jeҥ[nJKK[`-[F p8rrr0 xx SSS?~|a ?S(0aF|Hbq(M6|߿cƌ5kֳgψ#Gyyyohhuuu&L> ><66 ܸW,Q[[~Ĉ+V~}RRR}}=8l-]r/((;FZGGGmm-pEfϞ=c 𮢢r~kّgUH$RTT( ())ill,//Y\\}zbbb[[ZA$V &L\SSmѵ1\BX[[\R ٳ(PVV߿뛜cPX__;wؘn۶袧[>\4 3gfaaqΝY (++P' 1XG"L9rDRR ggڣG>b8p`FFF;w433 HVV@KKKQQA333A%%%W^gmmm ?p̙_QTT677711 hׯ_wފ+P,0 Z޿laay̙>OOM6>`̚5Jeeel61dII'A/666 (66ӧ;wTVV>w\hhl$@QFIJJ9sΝ;(@333#F`***y&;;[UUuӦMUUU6TTTtuu{{{㏠ yy#GܹNKKkcƌAnn.vN477622@!q}}}Y,8rȖMMM` AΝ;O>moog[[[---_G(&I={HŽ_JD*eh*AH/ɓ'O|=p~kk+=TF^re@P? 4s8Xi7 CN H^^yqc"? ht~tGqiOELac&iP9BP 흘jjj \/^ |?~T*pd0; M!s ǎ+w 555LBD͇s}_@( |s7 yysb]ttt|"֥SNNN__?-- ,@[c3'e* ƚA RQX_ %HUPMMM:u*oiiQVVhRTKQHώʒ={"X A)4}37-l?TB2X~R_~a1z\XZZZs-))AaIa˅IL-&M4iDA4ʋ8:@uB/ijjbRlʱ/4^C44ZlyqHS A*sڙ@ QPPtbJb&/ ;::Μ9cllP @Iփ ]hI"%-[ikGLURR7nܜ9sJ(Iڼb^ݺuLJ8PUp]*~қP" 399ӧO֭B(%%=~ף/MO!SwKKK 'B/d.ao3(C9P}mڴI 0f<4Dt60-p '[ZZX9h`IRM@ WWWjhh(**% K߱d>;*W(n߾]$>__hc;w2!!aP~FBlzp8+###IKKKIIGωh i9Q"'68֗vKc?(q8n|Gq>O-4I OHr| Я@b1mkk#IS55,,L$uww  ɟ_7o=~i ˕Ϟ=prrO4?A#8C ('/* ,?@3Uvu5 &L055E{P N2.s`bc^^ÉC61D522r߾}?BKR ƾl{@@q㢣 Icjc0|U_zN @3A08&11x=x=weddo޼ήŋ߯)¹m 3(@yM6Si$3p&&&T_{wX N'0 5''66`kk֭[siiiݻwڵk^^^>>>aaaIe^AbXFF_n]FF1&aMMM--- Ͽq֭[5GHSSS(jhh|RFF8aptt3fL||˗,XٙAPaaa^^:R聅qqqAAA}}}ʙX; Zh< zڵkDBD9y 0̣TMMM^^~̘18wHtM;;{{:;;ԈÇ?}ڵk~~~4Aqqq!!!ǎCO:o߾֭[ϟ?/++[dI}}dTWW A.ёx{ sbX***3fZ O  }}}===(3KHH޿?a ooo6]^^cǎѣG߽{Ν;aaa{=qzP/zX,>qDrr}}}̙cdd48 ȑ#߿A gΜqqq9v옣#C;QbRPPaٹ,x0l ˗/;::0'5kzxxVUU _ZZZYY)$@RRRT j+ B ;uTWW /O+Wvvv/Ǝ{ٸ8P`LӧO&&&^zuxx;rssΞ=J7oz{{{{{++qϚ5KBB EFF5*$$ŋ'N;wٳgwU[[y…%KԌ7nxƍ+W߼ySZZ gp܌e˖:u*T[[[WWWddgΜyرG2:z:tԩS:;;###Q/ggOx<\@z( ***֭{!4\777'''pXpppEEEWWڵk\bXCCLxϟ?&~f 8@߭@رc,ٳGfA'O$&&7oӑyyy1}t *Zv֪U G3C@eG]v˖-gϞ p8݋Yl(x?x`)))G1cƂ pk---'N`8ohh8g___l!''qժU'N@4551bċ/hĒ ="""//_~zA^X 300={vAAV~VVrA,2VUU%!!!!!M))ƍgll r ~bϞ=Ν )++;r0m4;7U>#fҸ@'uX ȑ#AVx͛7n())i``իAdmmmddfo߾}%===vqoo\kׂܩ& .,k…L}i.O>UTT8qbNN88 999o޼ (<<ٳg6mڱcsYYiiiQ$UZ Sppƍo߾xb?.H+M:e(..>z7@PRR9r$8~by{{xxx\z?.^楉m |V,WVV.Va7o?[ӦMC$O>v999 A vvv8ZEEaΨ.++3440Mܗ2228{{{nƍW^ݹs ***|}};::]O?:t@ѣ 8).yO>500Xj6 E(]am,ӧQuQQQK,X|իW9Ϸ% L;0@ ݻ...`144455?>N 1/aߞK.Ca6Ǐի{.]ˡMMM_z5 jii}'/̙3411aXK.^W^駟gVVӧE"Ҋ+Νp !5b`cMM 8D okk< /^hoo ѣG .?Ic^__H$ZjUoo ].ꀳlvhh#G:::ɓ'%%%0D T> .QF KHcDRR9M\ك?_%RWWsΕ+W&Oc///p7)Q$Jsvvᅰ߿4l Ǣƾ@m۶5k֠?smmm _xqׯ_+xYYY5 |)**m莌,))9qč7̙UUUURR`dd?Ԁ*++kjjq\SQQQCCïJUcMOOkhhx" î౳bʣjuQA޿2cƌaÆ5779r9::n޼y0 =,444̜9055LGG?~<5[ UUU5l00c&Q9yd]]ݚ5kt.Q i}kCBB֬Yz˖-^tiLL̙3 @_N,pARRϜ9=r ښ6rH p777*;Iʕ+vpb16zE"QqqqbbbRRRnn݅ dƝw Y HMM \?vww/]aH*ظgϞX7+((ݻ7==AEEEGرc=<<.\?CTYYY__ǂ07 WRR2,,hϞ=40 HYY٣GlG YjIJJ)S8쎎g?~\QQ1)))&& 6lXGsrr߿/PP}l|x11Xbγg'z' PQQQ]]]}͚5"==80ljjz՟ n¨j0 KII͘1ϟ?Eݻ7vطonڴ AO?:u*88xŊ0{ŋe˖kkk,\֭[Bz޼y׿?r䈧ɓ'> ð={^+VDDDL6mHܹsǏg?A%K<|PRRDII [UU0ډHHH:unBmTUUUTTпٵk˗/uuu;m\fڹs֭[nݺ|r7770OC`^ Jr\.w˖-f̙3&HFIK߿wG@:::#;uܷo0f̘1ƷoҒorʢ"ܷpTpJ\ P(ܱcǿ/pAQe E ssseeepHKA^5 wY[[655?^,z,@2vB |^pa͚5B|xm۶Vy !_`2@,r7oހ @]LĢ‡Ȁ###GЀcDmmm)))}ݎ;uG555ↆ_~<00~Xo8paӧ)S>|8## ip";⒘*Q\*˗/'qPD [n}]uuu```oo޽{v-.+##o>gggSSSYYلK I!&Mŋ6Eڵ gϢ[YY 3+nnnjjj>>>cƌihhx111gXXA`Qaȑ6fEEQFm~ы/nݺ{-[^~}֬Y_Xv-::Bd``ݍIA邋44T`CeRs~1c(** 윜 YYqYXXxxxeff?~\FFfX@nN cǎ۱c:$V\ 8EGGgddpzzz֯_ha-A!. pOq H!LB 0w޽pkkk,X --r@t7{l[[ÇڵKSSS$- D"ѣGM&Qccc`ӽ{,Yw߁)WQQQWW>y z{{W^ V]]]-[6rȦYfꦧlJCA\\\V^pItȩSN0-,, }||n޼ k׮cS4O{{{---3J!+&QOb* ;A+VڻwokkٳB%Kz1cFqqƍihGX˞(0sى'>|8** ;ر}eeeXTqٞX ;w. U}DEhkKTAʀ bl(,_~#+WM6l0t 1Bt֭)Sϟqƛ7o,[,;;ӧOPYFNNѣG &&&>>>7nܸqFww\TTǏbqOO0!!!%%L/˖-{{{B!S__LHII p\U=///## A/q4pHTIL3 X, ֭KKK|N,!uuuuww_ ,-- kGGG Y^^L8?/\L 0 FݽJGG7ڼdɒݻwpX,VTTܸq#6..]ݻqݼyׯ_ϟ6I}UtIHH ƎG,2{D.)UkT67eqTl+)'"t`޺uի544:m۶3gfss3QrkkW`` JJJ;wa$" aCCCwwwLq8 (<<<11O,aCg܀"jڴiwy&7/ ݻwDE%%%---aXZZY:{]vիq`^l*s4lX悂P(! Jņ\0 z˃u0۵k׀& 'V,o޼yහK"k޽kddʪgdd{(1?&`*A?0""UB|rB~l t }qssn\jmm 1cFMM z ~ǻwr'NNȄ .]BOո}yLH[[{١?.ddd >Dz555I7x ]2mڴi6lԩS/^!Bz]Ozn___yy}Cޮk.❊._j`` p$!!ZZ.&''744D!$1#dR)c tVV\vv6A}}}6W7zDɈ  pKKKGGz5 /P򾾾IcX 6׭[ ~X{޸q4X94ZXz{{ۣi3yd)))''#G#כ>'\0e&yFNd۷o?~|||OA8S6lpedd\]]mllP>aÆGi҈ IXG)//R%0 NOO=XF#r`/ k֬~BBX^ {ϳ1^Ἄ---m@)i@T4ir!)Sb`` C 3 !DjHߘDW^EСCӧO3gH auusbv` 4Tm: `cee铛%X@M81##_Z:3<O^^Ap(:N'R墫T^YYիE"6X;XBMHh0pݴiLDFFҀhbwڵ^vbƌ_H$2006gHJJj.]ټyI&UUU׳XI&Ar333-,,ׯ_P9Z8&Vw@TiF̙3eddXopLTGӷFR"ܙ2Nх ǍN䦧pmb3 R8! *ttt~ݍ] XPCN*ARRRBPAA`X]]]ըܮ.w"b@pT ۷755tvv~Jq%^*% NFc 2UbhQ NNNrrrh!TPPhll,))rCD^q)EBT`URRIj9DX @IF433KOOWVV}}}FFFǏ͝9s&p}:Q4@3|ts2R!@[[[g("5cp =bW·ND9vvv\.B*oT^nh Dnp=Sg2#~=F*_ZZpp a$Ď`KꈍC7ojjj, +*8i 12̥|4ᕕ/_嫬LHHXp7o444I}:jpiYIo BsT GE'y即XPqjkkXJQU DV@,K RTҰ6g3=@p)/b000PRRɓ20 ?ql4uU>b Ѣ>UĬKW1(bJl 0 HhX'''gggmIII222EEE3f̐%H4+$c 5N&UT vH(@pvO𞝝-''^ %s(<Y!k;p[cc#1,*$6z%( =vn9C6q*!www_OqaT{+J#o uŝ / Z0һhDkdb?iIV n6 b{Ca?M1iMMof\G:ĤNb%1qC%? !bTօIb{R~4`b-33/@KyZ!Hck*{uwLEZNZN0fRbU4H]*@y@MA#K> N= cOG , <0H%mImúcc 4RXi7 bhS&];/To”sRI{DqHEBRY4O755EDDpwޖ-[H"|/0USGB%}'DNK/B.T ߿,**z8CoW 1d M% ~v >EhذaqqqOmVZu%ļh S캺|p+pr tj]$넸v 𗺡!L=Cb?h4$---|~{{"nhhP-x9_,6Lb߱86bwg.)3}(&ҨDPW zR 4zP ##3{쒒kkkx-R@~XYI:@I+D0K DDĜGC 1*yAe_)@D*T0 [ZZf_41/HfPI&"}A$cBlL B '1H%vpEՅUD0#iFdOC!@bAyz6@>kTi}p jRhbV:@ʿ(p*'ҰOܯ}( X8uhՈu rHJXEvgUK?HQB mI ꘧/*CF1FKT)~:@z NZBDIUFn3.;1Uh1ISk\2hȡBRU45'V PZZfq. i4kp*zXG&*rdo 1!BQ_8E+F)--mhhZd A_ʦ|%ȼ+^"%Qq~/Q``` (!!\.744g;;;OuttN2|TVVN8|677s80yAPcccmmlhhr&L ޽{goo>೷Ç(KzzzYuuu塗 pʔ)G%р(//qmЉ8T|8qgϞ?l6;!!a߾} `RRRjj`xMzz]ׯsrr~gŋBoooVA]]]i&Y^^w9YRRrӧOO>ݼy\>AP~~ݻw?>sss>|x𙕕va𙑑Ӄ޽_ojj* f7oԯz5r:[WSSsq\UUui???6pT\\}SA ?{d+K KEe ~!Ԇ1!D;VKGGGVV?**BlN>|/jjjHa?cbb"$''УC***֧"lrG0 D"p). pB1 Ԥ&))}}}X,z9&yK( Y,vG^^ennnU;Cq%b1: .`F ýiSM0I߉HRI7K0 XR"DD̢(oʐpv_t1544=[PQ < ^B͛7nj#)IrTܵkRSSuuu&P痗geeE"000++ܜh Ï= H,Y \͛YYYs7oLOOWRR"4!ao߾!?~811‚xB0 >PQ(@\QDh#ߞ W-'"#P$e077///|2Ꞟ0|@ҵ7nmii? lܸիW8---xyy}gOKK )mmm}}}<>{ kjj666p߾}+ TlXZ[[֯_?|t@baa!80 }mmme7&&ۤ>bbb Ab Ld0, /]v,&X,ʒ}NZYYoܸ蛖}2kkkAo߾]f2zJ=4iQh<X~~~'Nܿ?1 VXaiiIxG1}touu9sO|Ic\(`|#H#*_`scc/R[[KwjRf__ߪ*R_~ʷe߾}!bqUUՙ3g#~%%%uuuWQQ)oFboDD~Px &‹?~ȄgAAAB0999==Ç2 __/^&M@@b" _A#q!0 Y?>MMMwojjjmm_z۷okkkwuuԘa}[[[tuu8ďRhhȑ# fdd 1b1722R__566!}䉎DgϞihh6l0fpll 8|ĉh ^^x,---Jy򥢢 Ϸ|o"RVVVss6Ø0z]]]>?}tzuUUUcǎcqqqaaY~8͵-//GL^M;.6jԨÇ+**Knnnwuu-**;w.3,0!! +**mب|P(,++۶m[SS[[[!˽o]]]ll, )++nnnF0ۛfa</))PNNnݺuD_VVVvÆ DԼJJJzzz}ٺu+W  ۷}߾}ѽ  >|E"Qffffffmm-bqfffFFFUUձc8&݃AÜSN˜\VVƄǏIII@rY~~7o ϝ;G"z >o^zu{{;vqqGyVZ֦1o<===788xժUNNN ,*900~jjjNNNذ<|0** \Ћ?~hѢ6A3XpaWWB~qTT… |>XE/X烫q6o=GQϟʆVYY믿º%%%߯WGs|||Ri޽/^ Ak544444KCClD{D"D"SyyܹsѾ^dׯK=z3Q|8(d2WCCVee%~n2 ܹsgMM͊3grT(>>>bss) wCCCN455ЧLFN+$%%,))䬨ˉ4R͛7ඡX,FR(411G._xU鄄+W@ɨ^a.] KSSS^P(.\MOO|BzQD鞞 8"e~YfdIIɱcd2م  JeeeR,fRa*++>,HXq>z(Ljkkb1Lt;vX}}ŋŇt̒ D"cިͫdb7o\]]qqqnnn<*MHHpuu}-Ǐnnn\)))<ۆɮ111үI{撒77P!̥{1nnnB$WVV߿?((HsMM;ώ%M s}}a:3 iWh*\\.G_cQBPr\]]ZE5^h&> foQ@s}}W& 'J͓N2[k2woW0WѺJؤ ]_*/VlKN/^=<L{$$d7kZCXlRG>n.yzuXQiPHA{ y&Ug:JCAdw!Y D~VjEDX'C *R (@Xʒ@DIP-D]*<K1oVZТ^šůP("o) yZB3WuqqA>:LT(Ώ2uY999>}j׮:ȉ(*!!ϕAAA:t(--iӦ EQ ݸxMMͷo߲|}}۴i2SRR^|YWWwMkk뀀j333XW^^nff+455ԀFH;pbx+bKpCN.uGL)Ҥ$MMMmllﯪ ӋܹBPUUDo߾---MNN֭`EE!(JMM%`oܸQZZjnn7طMJJ233S սq7RլYG) ֭(ĉ/_(jʔ)rZSSsȐ!!!!Gzeiii۶mK+ǏSRR9>֡C+V444o}֖amm],Y$??K.:thhhi:##o Bt4jE\رӧOE-_>>>EYYYM4)"">;;{„ {~}׮]Ԇ ٥K7oL0^ -ddd4rH)ڵA355 ر#xӧOcǎ8-UIXmmmBP(*** 3D%%%&&&%z?YZZ֪ihho&%%Ox._|֬YE9s&--mY0uuϟ?[XXdggI$]]]ERSS# MӽzQWW#_~fxUt*ў 6b>\TTԩS'www۷ow3f%H$rcccLE8iҤp[3%%҂{geeLFFFsrrX}Fù O2i5X,.((`=SUUU__&Hdff6D^MP(9$33S&Ձ*++ \0LbbbqqD"JsrrcFe-'\+J\^-(ɞ?ZV(2,::\MT0̇%[JV l{D7kF_Z Eir8Ѡef+ <"!ҝ REBouq2>o&3{ʐYDd&g 9x>rņfMUD a)ǙD,_Aڋ_ HUg"@f&KW(᫑?y}arrr޽;gΜ`'''2RE+%|R&'޼y3b~o T*MIIb?~hiiEأG---d2YYYT*( <+ kff"@^zGCCCCCaÆBRݻ (dUMM zLbJJJ^*++CCC)ڵґ& m TWWOjhh<<(X٧O(Qn߾-utt f``P(ܥK2dSf̘۫W5k߿")) H~zppٳ}˗/G]\\L{e˖544PUZZ r;~իٳvZ92cƌe˖{ T|9h͛nii`CCC|!h۷o?ݻw_~D"Ylӧ}||]&&OrHEEŖ-[V^.HTUU/^?4 ^8~8<77777FT\.?qD@@m6pEQZZZIIIp($$$77R,ߺukxx88(SSSp,0̬Y~L ===pQ -[6iZ*C|UPd"*X| xRL&0744 H000 + X !S.r4|ƍ004I˫Xx $lz!ʂ9rDl,5y.,^pd(~:YP%Q rTe(h!SSSo'$$H/E&dK.[,$V^g񨨨888x(Lrd4̄,fˁmC<,hHh( lT)&~ӧϾ}z?͛7/''ujؽ{:|zLFl<eEKVl,ݻsΚ5k[WW'X} &I-At >45KC-xb0#iLB?~JB"L'YU,<ypJ4O#%f)B(u`3bi 8@V6x|F~D։@J[!p Na@ ?#@~g}!h9䑀>7T|M\Ol+6 CQPBfTQ@o2/,ĝ4.[ )Nyhpbٌ& ^4{)b)]Fj lm@wuXe|=Uگj8 B*TWȵR"R3Ѽ<⫼:w;QR<OR&٬o |bїȅ_)!k P *7A"2'|D5 {whϟ_~mjj$$|9Ґj4 {~t^ߖ76Uȣ&d /))yU6m^x*((E!*kI D&hh:w uyrVŋ,##A&[`KYO4x)NWW> y& Dsx8GD$ܼf8Dd.}TWW3&33pΝ;ѣGh,֥f+%z8Üoߪw˗zEcǎ( .+Y|.!<Js6[%j 45ӧ}B*Usxh!qud'H ҁRxla@.;MxEX‰(3yDǢv ۏs2 L3+=N+$ϊ)*E2hUr~\qD\v_[n={v,8Z.{pˉpN`= e+r"V*7jDT @TH *mAI6"Xb *\h.khʋ/b1@rڴiÇO6mĈg|uG$u2wiȐ!Em@"_׿gF\呸h^I\pA#@cpxl>"`qq{ij./r)ZM6?~m6"L"ܟ\qøT4 acanݺUVVVTT o~Z=kbMxѡn``бcGVF4} ^t K@ww,.*vC$UUUMl@0?(. KYUUK.\vreyUWWsT!744U(2 dFEE]x7Ǐoii [7n7|m۶7zxx/L{yymݺmL` %+RDĉ+{YYYR.k}28::3g( }mppׯqf X풒8V'"u P\}}}?nccs/fHNdPhAbݻw_vMÇ/_|ȑ,*ŷsoЋD"T>nܸcff6p@cWsO矫W_Ƃr<33W;v,8ի񇛛ϟe!Muuu;wLLL,// "667׿"##64FF9h uDMdHtӧs꒓_~ |9`.///((3H0 ӡC_~Az_lj'~iݺu|g*O VjZ6lQ %J.]>}~zPHڂ N:եKI&ݻw͛7E|ɓ'.\pttDmv ~aaյ>|ZSScǎaÆꪨXXXtuɒ%4MxÇ{쩮{ҥKZBձKQTqqX,.--?|p~~~~~aeeezz9sݿH[KKˈkkk$sٳg(́+!˻v\]]͛wΝ#Fl߾}#Fk]yyҥKMMMY;w=zllܸqʕ)S<==iQ(-s`` n!Mb̋^bcΝ/rbboݺ;A*XbȐ! 0f95S4]]]^|{npJkfKJJ2d3I`d`Kxx8XI{Ղ4M+#Gºeee<رcM߾}׬Y啙I'MtE3X+Ǐ/^xc O:\ak׮mڴÇ̬ ytvv? "LVT*=t萡E &D,!ϟOrrҥKǏfs[n_M0رc~~~QQQ>}BgHEEEFrss6mڡC-᲍tuu]hԩS\B9"X7h>!....,,СTH2##\oaH7lz !>>~ƌ:::NNNRT__lI1==\xLJ0˗A]9s eeeVھ}sFOQTCCC۶mUUUa;`4xeȑeee`ÇN1cFvvX,޷ouV4CeԩvvvYYYuuuVڹs)Sd]vڵ3sW(ׯ3DfffR55~m„ ֭3g?ЫW/aÆ Θ1ʊKݻw%ɑ#GGzL&}jjjsmӦMvv ba.]LMMtrʕݭ[6lpqGryJJJ۶m͉())qww?{l@}vjhhXb߾}VB[^ igϞHRD{UV{xxzk>}ZWWdɒ+W$&&J|rvv6EQ1bĔ)SfΜP(6l؀ (77ѯ򐐐{pDi… .izܹO>uttjkkf 0qqqG8p:iHP(޽d===cǎ_zzz;vd555m۶mhh믿***޼y]cMMM\ѹs:dll秭k.z~~~NNN+w޹snܸeҤIΝ qvv7n̙3mll?\PP_PP 0́]ݫP(?ֺ#F|w b+WF\.///OJJr~駞={z*''g={422g4SvFGGGEEƾ~nrpp(**ZzuϞ=ׯ_3F@~ǎnj^@YaҔBZ'O.**oVXq޽{1EQE8p`۶mpʔ)࿁L&x`ooocc}mhh޶mEQg655={,fmmݽ{w}}ϟOHH066NMMuց:wLlB" >\SS<γI[[gϞ--- qL&d'Ox"bii9o< AǏ>}6l7oBCC p'N !hhhRծ]_>xذaf***2e SUU4i9x˙0jۻw/EEE;w ƣdzfΝ;2+Wnݺ5--mԨQgΜy_sɦ{qq?/8::Ra8sLpƍ͛7?sk׮1cp† jkkrٳgsrrh>dE4_TTtIu twÇ`_yi@t۷o_W֭`wo߾E<`wtgϞTXX8rH Hҋ/Ax񢲲rӦM^\tӧ?}(soV__Aڵd?C``ի:zh/]y۷LUڢćQц_W1i??cǎ>|eygիW;wb'd D*޽4hPtt4*䧟~f;;{׮]\+:i~򥛛*xˡ4i&رuss?~|jj]hh(:ivڵklll@<|0F YjUUUwra/0Ϝ9Va`j =vBɃѣGv݅zi# K޽{CO&Lgy?Hs쪫Y>}:MRt߾}`xvww?tPXXwwwwwp_[[  o޼!7nҥK`'%%m߾}@wqH$JKK{9K.!J555ϝ;ӧڵk 555MMMѶmCT ޽{?,,,;ӧONNN ;wҌP]i-zeeWX<*Z;ZG6mZ\\%KΟ?$J3'>,\pΝ.]9gϞ󋍍ķ(y6fmmmೣ ܼyǏgtpp- ??C>}wfϞ~6zbbbΝ555㏄mrCCCX0LPP[ׯ_KWX &$$DFF-ZegBBxaٲe .yE߿իW,a^xqʈ9{lCCCRj[H#W-777LV]]}/h3"11ʕ+Rb'Q*xąKRad2lْ߾}QSSdɒGȧi:22ի-*ŭ[&O ƕ޲e~7pYKK/))IMMݽ{wMMͅ lmm^ Z~='AtVV[?D ĀtܙLg߿ Ϟ= |۷+**`E|c937Pjq ģ>C*VUUV[8qU' Äz{{Q - xE- ???06s[\gddݻ7""bԩXJ8$o۶СC;vzxxddd\r|A}7o,//|B|!CfΜ fiv޼y+8\~x:o߾ N}B9/mÆj'Bbq`hUK!O])J| [6@6Jӆ?ϪW)<*"(***++K./gϞ1L7@u ##{P<)srrrڴi3Ə?~x,,,ڴi3w\hvuu.++H$+V(_EQ֭ )(((*,,L&୪?~<8 4" NΞ=;00088رcb^^f]]XP3fmbbO?^ri@qmڴ/))ᑢt2 ƣBEEEGGܹs7nErrrEAA2Kfoݴif+?^QQQ__O,mӦa|XSScbbNt SSӐ"RQk7YETBP iyd6$OHtdd E!<Ҹ^=.....nk׮&NQD5uԔ'O&y@%yȑhɉӊ&'44tƍBdB8T__Ƕm*} y(x2 SYY1>b F`_׀D"ъ+rMaaUN<9|σ07 ,<@Q?#""^zաCp7B`c("(a 踺x,H$=ztZZZ8?a45066כ7o_233 BN\ULnҡpB$&WAY՛*e;y*ݢ|˖-#ߔ_79,\E޼ynHtXWWWZZ z$q^l6@iv툈P͌sa֬YEqE/ ЇH5q0:5i z(☘:tŋbf-<8?Noߎۃe6$P4h8$̙'$2s*kUFk*q ah|K?*Q/ڛ ` ]_$ߢrBfC*ov?kvH47fZKn4`lW!+|NWơώC5| \L+6)g*5o(T|m6 q2J P8 ]uIFHSd*@3ƥ '_W)%ϏXE=Tu6 R[g.w}yt)Exg/]DJYlJ5s "F4x5rh|4(ӧWɰ$=zo(*;;ٳ qE777LKKv<,99$?~k.LHHwގ;@2..Ç[n7o<}i&" gg?$KKK{i,**:xɓ'AѣǏɼ<#GxaÆҿ;wۗ(TP(%G"&D$ ޽H$255U:t4p~:d ihh0_UUzzzPյkW}}}Xjnn޾}{433366ISSSSSSܹs׮]aĤ{0illleeFFF0ٱcG&bݻa{;wnݺiiie6m`R&Ġi[[[p^EQ+VxOLNN֭OgBÇVVV ١C"wYGGGsjj@|0''U^԰A'Oy7nP(:44|2f nī(e+( oۛwMT@DPp7~neee4M]&.]$ɖ?|AեRiBB­[yCTY???w_ZLfbb(qw>} py֭7oº۷oJOO2df== tAG|8o)))?6660a<0Һ'0GP(ݻ1cX-,,hի#( ZZZݺuן0aZSSW^999cǎ a^~ݧO5Fμyka޾}뛐n``㓐֣GuuuzRRcccvGssٳg 4ɞlff6k֬wӧO711:99522׿{)KojjQ۶mܹӯ_?hkaRRR@MfO\JQTFF}MM.6m<}TWW_rvvq ۷oϜ[UUծ](1PPPо}-[tرSNE"ѬYJJJ8"PEQ"D&ijj+**̙#b=a7o]6:: Jbcc ֭[ٳiӦAű9997n >}:[QQQ\\iӦPһwJJJLLL[WRRҡCR<_VVgkkGhCE}.KKK555p.&iiZMKC(uLm:8bE[+-$ aHWI)lB|@&nw[!ss~{]ðSNipM!ٖBP:>}t*"6`gRJ(r:; Tze G$ ;/~ۻ.Z,L&SRR rfロK Y9\:́@n~ 0k QuQPH :|@ G ?Z_A]>O@'&&n޼f|>m[V @0{_~AZX,@͛Lc5_A499]V;77BDvGF1u:`|LꮀDZ yh4j5jzǬٱ`0j@R;wP%/..޺u+ zșX,###h4Jdj af4A!>qF86LyR5?_~P(A~snr@ ilL&H$*I=p@iiÇkjjhT.+*++  w7xZXL"={JtHD*:W_}!ɐPH*\^z;Nzð2{^ɡ2==hyy;v{G^xTz3n 0X8^`bnnf>X,KR__gϞO?4ўI={ gmllhY^^6L |>jE"jLz>oh,̨T*PhX>H$RZZѣGuuu"l6.X[[tJr߾}_~%J4P_=i7H6z(0 uLl EQA[[[Ѩj=t֝;wX,T*r_nll$@ YQQad2+@h4DB&-//:u PV UUU寿:H SSSHl6KӧOT*`*}9sFTl@qݵRܹsG%Gܜ鬫3 bte~O <:Y__7LgΜj{mkkdLgjjܹs4fJ j---p8.\ʪR8}j5JLc(`r.\(--W|։ .!z-F^|>L_699p:::\.y0Ht3i:QFD0\__L%u;v_5U*41 HT>P(0 &@ E0>Ą\.B=== q3'YTիYL>LfիWrIP]H$Rkx.@,J-;<Eѕxh0,))O& d28qBWWWkH$ rmm-ut:Q~:9D(&5>o2V* сX,٬T*Zd9NѸQQQa4x(G,aRyy"IvY&nۍu\(nooOOOx~!N+fWVVn7 6nb1HqF{{(\y-}`Fs!L&S4'1bti*rx|&b##woi)oW\I$Lȑ#SSS rx<6x<p8LA&L&}wFIqʕCTZZz嵵T*l^d {a' o߾~X,lb8 BESuhT.R! 7`q]oooKK_ AБ#G>x<.Hn\.w:LF$nDbdfEu}q<8pVNA^n5D nnnbM8]+`uuO}MMMx\i<ω}"P','\',xv0e)8*|U3ZDeVaVϓc*"!%mǐt.gI}bSJɪCf>Ki蜕4> endobj 274 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 275 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 276 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 275 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 278 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure2.png) >> endobj 279 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 280 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 279 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 46375>> stream xw\:s" h&**6MFcQ+Xb* M@:ʡr?q޻Z{aA  >a s  kiX'O%狓F B@Du8R "&1τ pC? NP50PL!ڊob1)%Ep.(fmrz E#C@; }LGA,RW_`@X* MMM111Z . ϺW^wsݽ{±C^XXFjғ'O^DDDtvvill,..NMME㭸ĉ… @ ۷o &j|ww???Rh`;77 APUUՆ <==;dRzuMM =O`` x<X֧O(gjj* [nv 4'` iii$"f@/_looyNN=H$*--===EEE ?666EEEmmmUUU\.7;;677\^\\\YYp,Y t.\|8q j^mmmcccNNP(c{{;`Ņbᔔ eeeEEE{|>ӦMaaa---eee ;;ʾ⚚WWWWVV>}r X,tϿwׯQ;7m^BCC=z^pA$1I;4rRSS{zzHyZZZ|}}J"""b۶m 'tttݻw֬Yb8++ ׿Y[[T\\immQ\__ۛ';;;7n{nʕ lAAP(<&;;[ UVV655.bܗ/_bK,߽{7!!A,{zzbs'%%b^СC?-mmmffffff0 gff>xXAAPRRRoooKKKUU۷UUU_>iҤիW+((ܹsgƌ׮]p8Ϟ=3008x RRRzqGGիWӧ744ڵK$?>22l̘1Tkk)SD]v>wΝ𦦦//^xӧOv횲rBBBwwwdddyyyuu50233S,ϙ3G(khh\r$001c|}}>|8f̘@ ݻwǏeJJJlllyyP(|w}[^^󛚚>|YYYkk>dggGGGrܾ>]]]l%\A+HT__?}#F0TTT4vXw}6]UU H__ݻw%$$@IHH())illdX޽kmm}Ʌ ,XpB.n>[nuww'''C?{l@0u>,XYYYÇoooWWW3gTTT^|8nܸӧO;888pb=|nϞ=|>?**jӦM0 &&&Ι3SKK#$$#**իbܹsb8..֭[<ŋB˗>v옇 ÷o^hԭ[ VUUZ\\ jޏ?_999ֺAAA$cYӈ:666pN (//t)b^WWp8H$>pĉMLLl윙&..NYYyÆ JJJ ҥKl٢a==ްW^-[̙3g '..޹sd>,@wwwooouuu/CCCCCӧOo޼ѣGՅϞ=_wJKKO8q޼y999ظeeeoo UUխ[{n޼+)))p\. >}fŧO믿nݺA^vv6`>aP(##.zzzA믿JKKњ$\{{ڵk<<< ڲejjj>| Aɓ:33 6zxxxxxfggƊ+o^\\lcc3yd---444P,RCC3iҤuihh˗/=|||-[^QQݻ[[M6YZZ6qƍ1bȑ+W4irYY* 777//jeҥ[nJKK[`-[F p8rrr0 xx SSS?~|a ?S(0aF|Hbq(M6|߿cƌ5kֳgψ#Gyyyohhuuu&L> ><66 ܸW,Q[[~Ĉ+V~}RRR}}=8l-]r/((;FZGGGmm-pEfϞ=c 𮢢r~kّgUH$RTT( ())ill,//Y\\}zbbb[[ZA$V &L\SSmѵ1\BX[[\R ٳ(PVV߿뛜cPX__;wؘn۶袧[>\4 3gfaaqΝY (++P' 1XG"L9rDRR ggڣG>b8p`FFF;w433 HVV@KKKQQA333A%%%W^gmmm ?p̙_QTT677711 hׯ_wފ+P,0 Z޿laay̙>OOM6>`̚5Jeeel61dII'A/666 (66ӧ;wTVV>w\hhl$@QFIJJ9sΝ;(@333#F`***y&;;[UUuӦMUUU6TTTtuu{{{㏠ yy#GܹNKKkcƌAnn.vN477622@!q}}}Y,8rȖMMM` AΝ;O>moog[[[---_G(&I={HŽ_JD*eh*AH/ɓ'O|=p~kk+=TF^re@P? 4s8Xi7 CN H^^yqc"? ht~tGqiOELac&iP9BP 흘jjj \/^ |?~T*pd0; M!s ǎ+w 555LBD͇s}_@( |s7 yysb]ttt|"֥SNNN__?-- ,@[c3'e* ƚA RQX_ %HUPMMM:u*oiiQVVhRTKQHώʒ={"X A)4}37-l?TB2X~R_~a1z\XZZZs-))AaIa˅IL-&M4iDA4ʋ8:@uB/ijjbRlʱ/4^C44ZlyqHS A*sڙ@ QPPtbJb&/ ;::Μ9cllP @Iփ ]hI"%-[ikGLURR7nܜ9sJ(Iڼb^ݺuLJ8PUp]*~қP" 399ӧO֭B(%%=~ף/MO!SwKKK 'B/d.ao3(C9P}mڴI 0f<4Dt60-p '[ZZX9h`IRM@ WWWjhh(**% K߱d>;*W(n߾]$>__hc;w2!!aP~FBlzp8+###IKKKIIGωh i9Q"'68֗vKc?(q8n|Gq>O-4I OHr| Я@b1mkk#IS55,,L$uww  ɟ_7o=~i ˕Ϟ=prrO4?A#8C ('/* ,?@3Uvu5 &L055E{P N2.s`bc^^ÉC61D522r߾}?BKR ƾl{@@q㢣 Icjc0|U_zN @3A08&11x=x=weddo޼ήŋ߯)¹m 3(@yM6Si$3p&&&T_{wX N'0 5''66`kk֭[siiiݻwڵk^^^>>>aaaIe^AbXFF_n]FF1&aMMM--- Ͽq֭[5GHSSS(jhh|RFF8aptt3fL||˗,XٙAPaaa^^:R聅qqqAAA}}}ʙX; Zh< zڵkDBD9y 0̣TMMM^^~̘18wHtM;;{{:;;ԈÇ?}ڵk~~~4Aqqq!!!ǎCO:o߾֭[ϟ?/++[dI}}dTWW A.ёx{ sbX***3fZ O  }}}===(3KHH޿?a ooo6]^^cǎѣG߽{Ν;aaa{=qzP/zX,>qDrr}}}̙cdd48 ȑ#߿A gΜqqq9v옣#C;QbRPPaٹ,x0l ˗/;::0'5kzxxVUU _ZZZYY)$@RRRT j+ B ;uTWW /O+Wvvv/Ǝ{ٸ8P`LӧO&&&^zuxx;rssΞ=J7oz{{{{{++qϚ5KBB EFF5*$$ŋ'N;wٳgwU[[y…%KԌ7nxƍ+W߼ySZZ gp܌e˖:u*T[[[WWWddgΜyرG2:z:tԩS:;;###Q/ggOx<\@z( ***֭{!4\777'''pXpppEEEWWڵk\bXCCLxϟ?&~f 8@߭@رc,ٳGfA'O$&&7oӑyyy1}t *Zv֪U G3C@eG]v˖-gϞ p8݋Yl(x?x`)))G1cƂ pk---'N`8ohh8g___l!''qժU'N@4551bċ/hĒ ="""//_~zA^X 300={vAAV~VVrA,2VUU%!!!!!M))ƍgll r ~bϞ=Ν )++;r0m4;7U>#fҸ@'uX ȑ#AVx͛7n())i``իAdmmmddfo߾}%===vqoo\kׂܩ& .,k…L}i.O>UTT8qbNN88 999o޼ (<<ٳg6mڱcsYYiiiQ$UZ Sppƍo߾xb?.H+M:e(..>z7@PRR9r$8~by{{xxx\z?.^楉m |V,WVV.Va7o?[ӦMC$O>v999 A vvv8ZEEaΨ.++3440Mܗ2228{{{nƍW^ݹs ***|}};::]O?:t@ѣ 8).yO>500Xj6 E(]am,ӧQuQQQK,X|իW9Ϸ% L;0@ ݻ...`144455?>N 1/aߞK.Ca6Ǐի{.]ˡMMM_z5 jii}'/̙3411aXK.^W^駟gVVӧE"Ҋ+Νp !5b`cMM 8D okk< /^hoo ѣG .?Ic^__H$ZjUoo ].ꀳlvhh#G:::ɓ'%%%0D T> .QF KHcDRR9M\ك?_%RWWsΕ+W&Oc///p7)Q$Jsvvᅰ߿4l Ǣƾ@m۶5k֠?smmm _xqׯ_+xYYY5 |)**m莌,))9qč7̙UUUURR`dd?Ԁ*++kjjq\SQQQCCïJUcMOOkhhx" î౳bʣjuQA޿2cƌaÆ5779r9::n޼y0 =,444̜9055LGG?~<5[ UUU5l00c&Q9yd]]ݚ5kt.Q i}kCBB֬Yz˖-^tiLL̙3 @_N,pARRϜ9=r ښ6rH p777*;Iʕ+vpb16zE"QqqqbbbRRRnn݅ dƝw Y HMM \?vww/]aH*ظgϞX7+((ݻ7==AEEEGرc=<<.\?CTYYY__ǂ07 WRR2,,hϞ=40 HYY٣GlG YjIJJ)S8쎎g?~\QQ1)))&& 6lXGsrr߿/PP}l|x11Xbγg'z' PQQQ]]]}͚5"==80ljjz՟ n¨j0 KII͘1ϟ?Eݻ7vطonڴ AO?:u*88xŊ0{ŋe˖kkk,\֭[Bz޼y׿?r䈧ɓ'> ð={^+VDDDL6mHܹsǏg?A%K<|PRRDII [UU0ډHHH:unBmTUUUTTпٵk˗/uuu;m\fڹs֭[nݺ|r7770OC`^ Jr\.w˖-f̙3&HFIK߿wG@:::#;uܷo0f̘1ƷoҒorʢ"ܷpTpJ\ P(ܱcǿ/pAQe E ssseeepHKA^5 wY[[655?^,z,@2vB |^pa͚5B|xm۶Vy !_`2@,r7oހ @]LĢ‡Ȁ###GЀcDmmm)))}ݎ;uG555ↆ_~<00~Xo8paӧ)S>|8## ip";⒘*Q\*˗/'qPD [n}]uuu```oo޽{v-.+##o>gggSSSYYلK I!&Mŋ6Eڵ gϢ[YY 3+nnnjjj>>>cƌihhx111gXXA`Qaȑ6fEEQFm~ы/nݺ{-[^~}֬Y_Xv-::Bd``ݍIA邋44T`CeRs~1c(** 윜 YYqYXXxxxeff?~\FFfX@nN cǎ۱c:$V\ 8EGGgddpzzz֯_ha-A!. pOq H!LB 0w޽pkkk,X --r@t7{l[[ÇڵKSSS$- D"ѣGM&Qccc`ӽ{,Yw߁)WQQQWW>y z{{W^ V]]]-[6rȦYfꦧlJCA\\\V^pItȩSN0-,, }||n޼ k׮cS4O{{{---3J!+&QOb* ;A+VڻwokkٳB%Kz1cFqqƍihGX˞(0sى'>|8** ;ر}eeeXTqٞX ;w. U}DEhkKTAʀ bl(,_~#+WM6l0t 1Bt֭)Sϟqƛ7o,[,;;ӧOPYFNNѣG &&&>>>7nܸqFww\TTǏbqOO0!!!%%L/˖-{{{B!S__LHII p\U=///## A/q4pHTIL3 X, ֭KKK|N,!uuuuww_ ,-- kGGG Y^^L8?/\L 0 FݽJGG7ڼdɒݻwpX,VTTܸq#6..]ݻqݼyׯ_ϟ6I}UtIHH ƎG,2{D.)UkT67eqTl+)'"t`޺uի544:m۶3gfss3QrkkW`` JJJ;wa$" aCCCwwwLq8 (<<<11O,aCg܀"jڴiwy&7/ ݻwDE%%%---aXZZY:{]vիq`^l*s4lX悂P(! Jņ\0 z˃u0۵k׀& 'V,o޼yහK"k޽kddʪgdd{(1?&`*A?0""UB|rB~l t }qssn\jmm 1cFMM z ~ǻwr'NNȄ .]BOո}yLH[[{١?.ddd >Dz555I7x ]2mڴi6lԩS/^!Bz]Ozn___yy}Cޮk.❊._j`` p$!!ZZ.&''744D!$1#dR)c tVV\vv6A}}}6W7zDɈ  pKKKGGz5 /P򾾾IcX 6׭[ ~X{޸q4X94ZXz{{ۣi3yd)))''#G#כ>'\0e&yFNd۷o?~|||OA8S6lpedd\]]mllP>aÆGi҈ IXG)//R%0 NOO=XF#r`/ k֬~BBX^ {ϳ1^Ἄ---m@)i@T4ir!)Sb`` C 3 !DjHߘDW^EСCӧO3gH auusbv` 4Tm: `cee铛%X@M81##_Z:3<O^^Ap(:N'R墫T^YYիE"6X;XBMHh0pݴiLDFFҀhbwڵ^vbƌ_H$2006gHJJj.]ټyI&UUU׳XI&Ar333-,,ׯ_P9Z8&Vw@TiF̙3eddXopLTGӷFR"ܙ2Nх ǍN䦧pmb3 R8! *ttt~ݍ] XPCN*ARRRBPAA`X]]]ըܮ.w"b@pT ۷755tvv~Jq%^*% NFc 2UbhQ NNNrrrh!TPPhll,))rCD^q)EBT`URRIj9DX @IF433KOOWVV}}}FFFǏ͝9s&p}:Q4@3|ts2R!@[[[g("5cp =bW·ND9vvv\.B*oT^nh Dnp=Sg2#~=F*_ZZpp a$Ď`KꈍC7ojjj, +*8i 12̥|4ᕕ/_嫬LHHXp7o444I}:jpiYIo BsT GE'y即XPqjkkXJQU DV@,K RTҰ6g3=@p)/b000PRRɓ20 ?ql4uU>b Ѣ>UĬKW1(bJl 0 HhX'''gggmIII222EEE3f̐%H4+$c 5N&UT vH(@pvO𞝝-''^ %s(<Y!k;p[cc#1,*$6z%( =vn9C6q*!www_OqaT{+J#o uŝ / Z0һhDkdb?iIV n6 b{Ca?M1iMMof\G:ĤNb%1qC%? !bTօIb{R~4`b-33/@KyZ!Hck*{uwLEZNZN0fRbU4H]*@y@MA#K> N= cOG , <0H%mImúcc 4RXi7 bhS&];/To”sRI{DqHEBRY4O755EDDpwޖ-[H"|/0USGB%}'DNK/B.T ߿,**z8CoW 1d M% ~v >EhذaqqqOmVZu%ļh S캺|p+pr tj]$넸v 𗺡!L=Cb?h4$---|~{{"nhhP-x9_,6Lb߱86bwg.)3}(&ҨDPW zR 4zP ##3{쒒kkkx-R@~XYI:@I+D0K DDĜGC 1*yAe_)@D*T0 [ZZf_41/HfPI&"}A$cBlL B '1H%vpEՅUD0#iFdOC!@bAyz6@>kTi}p jRhbV:@ʿ(p*'ҰOܯ}( X8uhՈu rHJXEvgUK?HQB mI ꘧/*CF1FKT)~:@z NZBDIUFn3.;1Uh1ISk\2hȡBRU45'V PZZfq. i4kp*zXG&*rdo 1!BQ_8E+F)--mhhZd A_ʦ|%ȼ+^"%Qq~/Q``` (!!\.744g;;;OuttN2|TVVN8|677s80yAPcccmmlhhr&L ޽{goo>೷Ç(KzzzYuuu塗 pʔ)G%р(//qmЉ8T|8qgϞ?l6;!!a߾} `RRRjj`xMzz]ׯsrr~gŋBoooVA]]]i&Y^^w9YRRrӧOO>ݼy\>AP~~ݻw?>sss>|x𙕕va𙑑Ӄ޽_ojj* f7oԯz5r:[WSSsq\UUui???6pT\\}SA ?{d+K KEe ~!Ԇ1!D;VKGGGVV?**BlN>|/jjjHa?cbb"$''УC***֧"lrG0 D"p). pB1 Ԥ&))}}}X,z9&yK( Y,vG^^ennnU;Cq%b1: .`F ýiSM0I߉HRI7K0 XR"DD̢(oʐpv_t1544=[PQ < ^B͛7nj#)IrTܵkRSSuuu&P痗geeE"000++ܜh Ï= H,Y \͛YYYs7oLOOWRR"4!ao߾!?~811‚xB0 >PQ(@\QDh#ߞ W-'"#P$e077///|2Ꞟ0|@ҵ7nmii? lܸիW8---xyy}gOKK )mmm}}}<>{ kjj666p߾}+ TlXZ[[֯_?|t@baa!80 }mmme7&&ۤ>bbb Ab Ld0, /]v,&X,ʒ}NZYYoܸ蛖}2kkkAo߾]f2zJ=4iQh<X~~~'Nܿ?1 VXaiiIxG1}touu9sO|Ic\(`|#H#*_`scc/R[[KwjRf__ߪ*R_~ʷe߾}!bqUUՙ3g#~%%%uuuWQQ)oFboDD~Px &‹?~ȄgAAAB0999==Ç2 __/^&M@@b" _A#q!0 Y?>MMMwojjjmm_z۷okkkwuuԘa}[[[tuu8ďRhhȑ# fdd 1b1722R__566!}䉎DgϞihh6l0fpll 8|ĉh ^^x,---Jy򥢢 Ϸ|o"RVVVss6Ø0z]]]>?}tzuUUUcǎcqqqaaY~8͵-//GL^M;.6jԨÇ+**Knnnwuu-**;w.3,0!! +**mب|P(,++۶m[SS[[[!˽o]]]ll, )++nnnF0ۛfa</))PNNnݺuD_VVVvÆ DԼJJJzzz}ٺu+W  ۷}߾}ѽ  >|E"Qffffffmm-bqfffFFFUUձc8&݃AÜSN˜\VVƄǏIII@rY~~7o ϝ;G"z >o^zu{{;vqqGyVZ֦1o<===788xժUNNN ,*900~jjjNNNذ<|0** \Ћ?~hѢ6A3XpaWWB~qTT… |>XE/X烫q6o=GQϟʆVYY믿º%%%߯WGs|||Ri޽/^ Ak544444KCClD{D"D"SyyܹsѾ^dׯK=z3Q|8(d2WCCVee%~n2 ܹsgMM͊3grT(>>>bss) wCCCN455ЧLFN+$%%,))䬨ˉ4R͛7ඡX,FR(411G._xU鄄+W@ɨ^a.] KSSS^P(.\MOO|BzQD鞞 8"e~YfdIIɱcd2م  JeeeR,fRa*++>,HXq>z(Ljkkb1Lt;vX}}ŋŇt̒ D"cިͫdb7o\]]qqqnnn<*MHHpuu}-Ǐnnn\)))<ۆɮ111үI{撒77P!̥{1nnnB$WVV߿?((HsMM;ώ%M s}}a:3 iWh*\\.G_cQBPr\]]ZE5^h&> foQ@s}}W& 'J͓N2[k2woW0WѺJؤ ]_*/VlKN/^=<L{$$d7kZCXlRG>n.yzuXQiPHA{ y&Ug:JCAdw!Y D~VjEDX'C *R (@Xʒ@DIP-D]*<K1oVZТ^šůP("o) yZB3WuqqA>:LT(Ώ2uY999>}j׮:ȉ(*!!ϕAAA:t(--iӦ EQ ݸxMMͷo߲|}}۴i2SRR^|YWWwMkk뀀j333XW^^nff+455ԀFH;pbx+bKpCN.uGL)Ҥ$MMMmllﯪ ӋܹBPUUDo߾---MNN֭`EE!(JMM%`oܸQZZjnn7طMJJ233S սq7RլYG) ֭(ĉ/_(jʔ)rZSSsȐ!!!!Gzeiii۶mK+ǏSRR9>֡C+V444o}֖amm],Y$??K.:thhhi:##o Bt4jE\رӧOE-_>>>EYYYM4)"">;;{„ {~}׮]Ԇ ٥K7oL0^ -ddd4rH)ڵA355 ر#xӧOcǎ8-UIXmmmBP(*** 3D%%%&&&%z?YZZ֪ihho&%%Ox._|֬YE9s&--mY0uuϟ?[XXdggI$]]]ERSS# MӽzQWW#_~fxUt*ў 6b>\TTԩS'www۷ow3f%H$rcccLE8iҤp[3%%҂{geeLFFFsrrX}Fù O2i5X,.((`=SUUU__&Hdff6D^MP(9$33S&Ձ*++ \0LbbbqqD"JsrrcFe-'\+J\^-(ɞ?ZV(2,::\MT0̇%[JV l{D7kF_Z Eir8Ѡef+ <"!ҝ REBouq2>o&3{ʐYDd&g 9x>rņfMUD a)ǙD,_Aڋ_ HUg"@f&KW(᫑?y}arrr޽;gΜ`'''2RE+%|R&'޼y3b~o T*MIIb?~hiiEأG---d2YYYT*( <+ kff"@^zGCCCCCaÆBRݻ (dUMM zLbJJJ^*++CCC)ڵґ& m TWWOjhh<<(X٧O(Qn߾-utt f``P(ܥK2dSf̘۫W5k߿")) H~zppٳ}˗/G]\\L{e˖544PUZZ r;~իٳvZ92cƌe˖{ T|9h͛nii`CCC|!h۷o?ݻw_~D"Ylӧ}||]&&OrHEEŖ-[V^.HTUU/^?4 ^8~8<77777FT\.?qD@@m6pEQZZZIIIp($$$77R,ߺukxx88(SSSp,0̬Y~L ===pQ -[6iZ*C|UPd"*X| xRL&0744 H000 + X !S.r4|ƍ004I˫Xx $lz!ʂ9rDl,5y.,^pd(~:YP%Q rTe(h!SSSo'$$H/E&dK.[,$V^g񨨨888x(Lrd4̄,fˁmC<,hHh( lT)&~ӧϾ}z?͛7/''ujؽ{:|zLFl<eEKVl,ݻsΚ5k[WW'X} &I-At >45KC-xb0#iLB?~JB"L'YU,<ypJ4O#%f)B(u`3bi 8@V6x|F~D։@J[!p Na@ ?#@~g}!h9䑀>7T|M\Ol+6 CQPBfTQ@o2/,ĝ4.[ )Nyhpbٌ& ^4{)b)]Fj lm@wuXe|=Uگj8 B*TWȵR"R3Ѽ<⫼:w;QR<OR&٬o |bїȅ_)!k P *7A"2'|D5 {whϟ_~mjj$$|9Ґj4 {~t^ߖ76Uȣ&d /))yU6m^x*((E!*kI D&hh:w uyrVŋ,##A&[`KYO4x)NWW> y& Dsx8GD$ܼf8Dd.}TWW3&33pΝ;ѣGh,֥f+%z8Üoߪw˗zEcǎ( .+Y|.!<Js6[%j 45ӧ}B*Usxh!qud'H ҁRxla@.;MxEX‰(3yDǢv ۏs2 L3+=N+$ϊ)*E2hUr~\qD\v_[n={v,8Z.{pˉpN`= e+r"V*7jDT @TH *mAI6"Xb *\h.khʋ/b1@rڴiÇO6mĈg|uG$u2wiȐ!Em@"_׿gF\呸h^I\pA#@cpxl>"`qq{ij./r)ZM6?~m6"L"ܟ\qøT4 acanݺUVVVTT o~Z=kbMxѡn``бcGVF4} ^t K@ww,.*vC$UUUMl@0?(. KYUUK.\vreyUWWsT!744U(2 dFEE]x7Ǐoii [7n7|m۶7zxx/L{yymݺmL` %+RDĉ+{YYYR.k}28::3g( }mppׯqf X풒8V'"u P\}}}?nccs/fHNdPhAbݻw_vMÇ/_|ȑ,*ŷsoЋD"T>nܸcff6p@cWsO矫W_Ƃr<33W;v,8ի񇛛ϟe!Muuu;wLLL,// "667׿"##64FF9h uDMdHtӧs꒓_~ |9`.///((3H0 ӡC_~Az_lj'~iݺu|g*O VjZ6lQ %J.]>}~zPHڂ N:եKI&ݻw͛7E|ɓ'.\pttDmv ~aaյ>|ZSScǎaÆꪨXXXtuɒ%4MxÇ{쩮{ҥKZBձKQTqqX,.--?|p~~~~~aeeezz9sݿH[KKˈkkk$sٳg(́+!˻v\]]͛wΝ#Fl߾}#Fk]yyҥKMMMY;w=zllܸqʕ)S<==iQ(-s`` n!Mb̋^bcΝ/rbboݺ;A*XbȐ! 0f95S4]]]^|{npJkfKJJ2d3I`d`Kxx8XI{Ղ4M+#Gºeee<رcM߾}׬Y啙I'MtE3X+Ǐ/^xc O:\ak׮mڴÇ̬ ytvv? "LVT*=t萡E &D,!ϟOrrҥKǏfs[n_M0رc~~~QQQ>}BgHEEEFrss6mڡC-᲍tuu]hԩS\B9"X7h>!....,,СTH2##\oaH7lz !>>~ƌ:::NNNRT__lI1==\xLJ0˗A]9s eeeVھ}sFOQTCCC۶mUUUa;`4xeȑeee`ÇN1cFvvX,޷ouV4CeԩvvvYYYuuuVڹs)Sd]vڵ3sW(ׯ3DfffR55~m„ ֭3g?ЫW/aÆ Θ1ʊKݻw%ɑ#GGzL&}jjjsmӦMvv ba.]LMMtrʕݭ[6lpqGryJJJ۶m͉())qww?{l@}vjhhXb߾}VB[^ igϞHRD{UV{xxzk>}ZWWdɒ+W$&&J|rvv6EQ1bĔ)SfΜP(6l؀ (77ѯ򐐐{pDi… .izܹO>uttjkkf 0qqqG8p:iHP(޽d===cǎ_zzz;vd555m۶mhh믿***޼y]cMMM\ѹs:dll秭k.z~~~NNN+w޹snܸeҤIΝ qvv7n̙3mll?\PP_PP 0́]ݫP(?ֺ#F|w b+WF\.///OJJr~駞={z*''g={422g4SvFGGGEEƾ~nrpp(**ZzuϞ=ׯ_3F@~ǎnj^@YaҔBZ'O.**oVXq޽{1EQE8p`۶mpʔ)࿁L&x`ooocc}mhh޶mEQg655={,fmmݽ{w}}ϟOHH066NMMuց:wLlB" >\SS<γI[[gϞ--- qL&d'Ox"bii9o< AǏ>}6l7oBCC p'N !hhhRծ]_>xذaf***2e SUU4i9x˙0jۻw/EEE;w ƣdzfΝ;2+Wnݺ5--mԨQgΜy_sɦ{qq?/8::Ra8sLpƍ͛7?sk׮1cp† jkkrٳgsrrh>dE4_TTtIu twÇ`_yi@t۷o_W֭`wo߾E<`wtgϞTXX8rH Hҋ/Ax񢲲rӦM^\tӧ?}(soV__Aڵd?C``ի:zh/]y۷LUڢćQц_W1i??cǎ>|eygիW;wb'd D*޽4hPtt4*䧟~f;;{׮]\+:i~򥛛*xˡ4i&رuss?~|jj]hh(:ivڵklll@<|0F YjUUUwra/0Ϝ9Va`j =vBɃѣGv݅zi# K޽{CO&Lgy?Hs쪫Y>}:MRt߾}`xvww?tPXXwwwwwp_[[  o޼!7nҥK`'%%m߾}@wqH$JKK{9K.!J555ϝ;ӧڵk 555MMMѶmCT ޽{?,,,;ӧONNN ;wҌP]i-zeeWX<*Z;ZG6mZ\\%KΟ?$J3'>,\pΝ.]9gϞ󋍍ķ(y6fmmmೣ ܼyǏgtpp- ??C>}wfϞ~6zbbbΝ555㏄mrCCCX0LPP[ׯ_KWX &$$DFF-ZegBBxaٲe .yE߿իW,a^xqʈ9{lCCCRj[H#W-777LV]]}/h3"11ʕ+Rb'Q*xąKRad2lْ߾}QSSdɒGȧi:22ի-*ŭ[&O ƕ޲e~7pYKK/))IMMݽ{wMMͅ lmm^ Z~='AtVV[?D ĀtܙLg߿ Ϟ= |۷+**`E|c937Pjq ģ>C*VUUV[8qU' Äz{{Q - xE- ???06s[\gddݻ7""bԩXJ8$o۶СC;vzxxddd\r|A}7o,//|B|!CfΜ fiv޼y+8\~x:o߾ N}B9/mÆj'Bbq`hUK!O])J| [6@6Jӆ?ϪW)<*"(***++K./gϞ1L7@u ##{P<)srrrڴi3Ə?~x,,,ڴi3w\hvuu.++H$+V(_EQ֭ )(((*,,L&୪?~<8 4" NΞ=;00088رcb^^f]]XP3fmbbO?^ri@qmڴ/))ᑢt2 ƣBEEEGGܹs7nErrrEAA2Kfoݴif+?^QQQ__O,mӦa|XSScbbNt SSӐ"RQk7YETBP iyd6$OHtdd E!<Ҹ^=.....nk׮&NQD5uԔ'O&y@%yȑhɉӊ&'44tƍBdB8T__Ƕm*} y(x2 SYY1>b F`_׀D"ъ+rMaaUN<9|σ07 ,<@Q?#""^zաCp7B`c("(a 踺x,H$=ztZZZ8?a45066כ7o_233 BN\ULnҡpB$&WAY՛*e;y*ݢ|˖-#ߔ_79,\E޼ynHtXWWWZZ z$q^l6@iv툈P͌sa֬YEqE/ ЇH5q0:5i z(☘:tŋbf-<8?Noߎۃe6$P4h8$̙'$2s*kUFk*q ah|K?*Q/ڛ ` ]_$ߢrBfC*ov?kvH47fZKn4`lW!+|NWơώC5| \L+6)g*5o(T|m6 q2J P8 ]uIFHSd*@3ƥ '_W)%ϏXE=Tu6 R[g.w}yt)Exg/]DJYlJ5s "F4x5rh|4(ӧWɰ$=zo(*;;ٳ qE777LKKv<,99$?~k.LHHwގ;@2..Ç[n7o<}i&" gg?$KKK{i,**:xɓ'AѣǏɼ<#GxaÆҿ;wۗ(TP(%G"&D$ ޽H$255U:t4p~:d ihh0_UUzzzPյkW}}}Xjnn޾}{433366ISSSSSSܹs׮]aĤ{0illleeFFF0ٱcG&bݻa{;wnݺiiie6m`R&Ġi[[[p^EQ+VxOLNN֭OgBÇVVV ١C"wYGGGsjj@|0''U^԰A'Oy7nP(:44|2f nī(e+( oۛwMT@DPp7~neee4M]&.]$ɖ?|AեRiBB­[yCTY???w_ZLfbb(qw>} py֭7oº۷oJOO2df== tAG|8o)))?6660a<0Һ'0GP(ݻ1cX-,,hի#( ZZZݺuן0aZSSW^999cǎ a^~ݧO5Fμyka޾}뛐n``㓐֣GuuuzRRcccvGssٳg 4ɞlff6k֬wӧO711:99522׿{)KojjQ۶mܹӯ_?hkaRRR@MfO\JQTFF}MM.6m<}TWW_rvvq ۷oϜ[UUծ](1PPPо}-[tرSNE"ѬYJJJ8"PEQ"D&ijj+**̙#b=a7o]6:: Jbcc ֭[ٳiӦAű9997n >}:[QQQ\\iӦPһwJJJLLL[WRRҡCR<_VVgkkGhCE}.KKK555p.&iiZMKC(uLm:8bE[+-$ aHWI)lB|@&nw[!ss~{]ðSNipM!ٖBP:>}t*"6`gRJ(r:; Tze G$ ;/~ۻ.Z,L&SRR rfロK Y9\:́@n~ 0k QuQPH :|@ G ?Z_A]>O@'&&n޼f|>m[V @0{_~AZX,@͛Lc5_A499]V;77BDvGF1u:`|LꮀDZ yh4j5jzǬٱ`0j@R;wP%/..޺u+ zșX,###h4Jdj af4A!>qF86LyR5?_~P(A~snr@ ilL&H$*I=p@iiÇkjjhT.+*++  w7xZXL"={JtHD*:W_}!ɐPH*\^z;Nzð2{^ɡ2==hyy;v{G^xTz3n 0X8^`bnnf>X,KR__gϞO?4ўI={ gmllhY^^6L |>jE"jLz>oh,̨T*PhX>H$RZZѣGuuu"l6.X[[tJr߾}_~%J4P_=i7H6z(0 uLl EQA[[[Ѩj=t֝;wX,T*r_nll$@ YQQad2+@h4DB&-//:u PV UUU寿:H SSSHl6KӧOT*`*}9sFTl@qݵRܹsG%Gܜ鬫3 bte~O <:Y__7LgΜj{mkkdLgjjܹs4fJ j---p8.\ʪR8}j5JLc(`r.\(--W|։ .!z-F^|>L_699p:::\.y0Ht3i:QFD0\__L%u;v_5U*41 HT>P(0 &@ E0>Ą\.B=== q3'YTիYL>LfիWrIP]H$Rkx.@,J-;<Eѕxh0,))O& d28qBWWWkH$ rmm-ut:Q~:9D(&5>o2V* сX,٬T*Zd9NѸQQQa4x(G,aRyy"IvY&nۍu\(nooOOOx~!N+fWVVn7 6nb1HqF{{(\y-}`Fs!L&S4'1bti*rx|&b##woi)oW\I$Lȑ#SSS rx<6x<p8LA&L&}wFIqʕCTZZz嵵T*l^d {a' o߾~X,lb8 BESuhT.R! 7`q]oooKK_ AБ#G>x<.Hn\.w:LF$nDbdfEu}q<8pVNA^n5D nnnbM8]+`uuO}MMMx\i<ω}"P','\',xv0e)8*|U3ZDeVaVϓc*"!%mǐt.gI}bSJɪCf>Ki蜕4> endobj 282 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 283 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 284 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 283 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 286 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure2.png) >> endobj 287 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 288 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 287 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 46375>> stream xw\:s" h&**6MFcQ+Xb* M@:ʡr?q޻Z{aA  >a s  kiX'O%狓F B@Du8R "&1τ pC? NP50PL!ڊob1)%Ep.(fmrz E#C@; }LGA,RW_`@X* MMM111Z . ϺW^wsݽ{±C^XXFjғ'O^DDDtvvill,..NMME㭸ĉ… @ ۷o &j|ww???Rh`;77 APUUՆ <==;dRzuMM =O`` x<X֧O(gjj* [nv 4'` iii$"f@/_looyNN=H$*--===EEE ?666EEEmmmUUU\.7;;677\^\\\YYp,Y t.\|8q j^mmmcccNNP(c{{;`Ņbᔔ eeeEEE{|>ӦMaaa---eee ;;ʾ⚚WWWWVV>}r X,tϿwׯQ;7m^BCC=z^pA$1I;4rRSS{zzHyZZZ|}}J"""b۶m 'tttݻw֬Yb8++ ׿Y[[T\\immQ\__ۛ';;;7n{nʕ lAAP(<&;;[ UVV655.bܗ/_bK,߽{7!!A,{zzbs'%%b^СC?-mmmffffff0 gff>xXAAPRRRoooKKKUU۷UUU_>iҤիW+((ܹsgƌ׮]p8Ϟ=3008x RRRzqGGիWӧ744ڵK$?>22l̘1Tkk)SD]v>wΝ𦦦//^xӧOv횲rBBBwwwdddyyyuu50233S,ϙ3G(khh\r$001c|}}>|8f̘@ ݻwǏeJJJlllyyP(|w}[^^󛚚>|YYYkk>dggGGGrܾ>]]]l%\A+HT__?}#F0TTT4vXw}6]UU H__ݻw%$$@IHH())illdX޽kmm}Ʌ ,XpB.n>[nuww'''C?{l@0u>,XYYYÇoooWWW3gTTT^|8nܸӧO;888pb=|nϞ=|>?**jӦM0 &&&Ι3SKK#$$#**իbܹsb8..֭[<ŋB˗>v옇 ÷o^hԭ[ VUUZ\\ jޏ?_999ֺAAA$cYӈ:666pN (//t)b^WWp8H$>pĉMLLl윙&..NYYyÆ JJJ ҥKl٢a==ްW^-[̙3g '..޹sd>,@wwwooouuu/CCCCCӧOo޼ѣGՅϞ=_wJKKO8q޼y999ظeeeoo UUխ[{n޼+)))p\. >}fŧO믿nݺA^vv6`>aP(##.zzzA믿JKKњ$\{{ڵk<<< ڲejjj>| Aɓ:33 6zxxxxxfggƊ+o^\\lcc3yd---444P,RCC3iҤuihh˗/=|||-[^QQݻ[[M6YZZ6qƍ1bȑ+W4irYY* 777//jeҥ[nJKK[`-[F p8rrr0 xx SSS?~|a ?S(0aF|Hbq(M6|߿cƌ5kֳgψ#Gyyyohhuuu&L> ><66 ܸW,Q[[~Ĉ+V~}RRR}}=8l-]r/((;FZGGGmm-pEfϞ=c 𮢢r~kّgUH$RTT( ())ill,//Y\\}zbbb[[ZA$V &L\SSmѵ1\BX[[\R ٳ(PVV߿뛜cPX__;wؘn۶袧[>\4 3gfaaqΝY (++P' 1XG"L9rDRR ggڣG>b8p`FFF;w433 HVV@KKKQQA333A%%%W^gmmm ?p̙_QTT677711 hׯ_wފ+P,0 Z޿laay̙>OOM6>`̚5Jeeel61dII'A/666 (66ӧ;wTVV>w\hhl$@QFIJJ9sΝ;(@333#F`***y&;;[UUuӦMUUU6TTTtuu{{{㏠ yy#GܹNKKkcƌAnn.vN477622@!q}}}Y,8rȖMMM` AΝ;O>moog[[[---_G(&I={HŽ_JD*eh*AH/ɓ'O|=p~kk+=TF^re@P? 4s8Xi7 CN H^^yqc"? ht~tGqiOELac&iP9BP 흘jjj \/^ |?~T*pd0; M!s ǎ+w 555LBD͇s}_@( |s7 yysb]ttt|"֥SNNN__?-- ,@[c3'e* ƚA RQX_ %HUPMMM:u*oiiQVVhRTKQHώʒ={"X A)4}37-l?TB2X~R_~a1z\XZZZs-))AaIa˅IL-&M4iDA4ʋ8:@uB/ijjbRlʱ/4^C44ZlyqHS A*sڙ@ QPPtbJb&/ ;::Μ9cllP @Iփ ]hI"%-[ikGLURR7nܜ9sJ(Iڼb^ݺuLJ8PUp]*~қP" 399ӧO֭B(%%=~ף/MO!SwKKK 'B/d.ao3(C9P}mڴI 0f<4Dt60-p '[ZZX9h`IRM@ WWWjhh(**% K߱d>;*W(n߾]$>__hc;w2!!aP~FBlzp8+###IKKKIIGωh i9Q"'68֗vKc?(q8n|Gq>O-4I OHr| Я@b1mkk#IS55,,L$uww  ɟ_7o=~i ˕Ϟ=prrO4?A#8C ('/* ,?@3Uvu5 &L055E{P N2.s`bc^^ÉC61D522r߾}?BKR ƾl{@@q㢣 Icjc0|U_zN @3A08&11x=x=weddo޼ήŋ߯)¹m 3(@yM6Si$3p&&&T_{wX N'0 5''66`kk֭[siiiݻwڵk^^^>>>aaaIe^AbXFF_n]FF1&aMMM--- Ͽq֭[5GHSSS(jhh|RFF8aptt3fL||˗,XٙAPaaa^^:R聅qqqAAA}}}ʙX; Zh< zڵkDBD9y 0̣TMMM^^~̘18wHtM;;{{:;;ԈÇ?}ڵk~~~4Aqqq!!!ǎCO:o߾֭[ϟ?/++[dI}}dTWW A.ёx{ sbX***3fZ O  }}}===(3KHH޿?a ooo6]^^cǎѣG߽{Ν;aaa{=qzP/zX,>qDrr}}}̙cdd48 ȑ#߿A gΜqqq9v옣#C;QbRPPaٹ,x0l ˗/;::0'5kzxxVUU _ZZZYY)$@RRRT j+ B ;uTWW /O+Wvvv/Ǝ{ٸ8P`LӧO&&&^zuxx;rssΞ=J7oz{{{{{++qϚ5KBB EFF5*$$ŋ'N;wٳgwU[[y…%KԌ7nxƍ+W߼ySZZ gp܌e˖:u*T[[[WWWddgΜyرG2:z:tԩS:;;###Q/ggOx<\@z( ***֭{!4\777'''pXpppEEEWWڵk\bXCCLxϟ?&~f 8@߭@رc,ٳGfA'O$&&7oӑyyy1}t *Zv֪U G3C@eG]v˖-gϞ p8݋Yl(x?x`)))G1cƂ pk---'N`8ohh8g___l!''qժU'N@4551bċ/hĒ ="""//_~zA^X 300={vAAV~VVrA,2VUU%!!!!!M))ƍgll r ~bϞ=Ν )++;r0m4;7U>#fҸ@'uX ȑ#AVx͛7n())i``իAdmmmddfo߾}%===vqoo\kׂܩ& .,k…L}i.O>UTT8qbNN88 999o޼ (<<ٳg6mڱcsYYiiiQ$UZ Sppƍo߾xb?.H+M:e(..>z7@PRR9r$8~by{{xxx\z?.^楉m |V,WVV.Va7o?[ӦMC$O>v999 A vvv8ZEEaΨ.++3440Mܗ2228{{{nƍW^ݹs ***|}};::]O?:t@ѣ 8).yO>500Xj6 E(]am,ӧQuQQQK,X|իW9Ϸ% L;0@ ݻ...`144455?>N 1/aߞK.Ca6Ǐի{.]ˡMMM_z5 jii}'/̙3411aXK.^W^駟gVVӧE"Ҋ+Νp !5b`cMM 8D okk< /^hoo ѣG .?Ic^__H$ZjUoo ].ꀳlvhh#G:::ɓ'%%%0D T> .QF KHcDRR9M\ك?_%RWWsΕ+W&Oc///p7)Q$Jsvvᅰ߿4l Ǣƾ@m۶5k֠?smmm _xqׯ_+xYYY5 |)**m莌,))9qč7̙UUUURR`dd?Ԁ*++kjjq\SQQQCCïJUcMOOkhhx" î౳bʣjuQA޿2cƌaÆ5779r9::n޼y0 =,444̜9055LGG?~<5[ UUU5l00c&Q9yd]]ݚ5kt.Q i}kCBB֬Yz˖-^tiLL̙3 @_N,pARRϜ9=r ښ6rH p777*;Iʕ+vpb16zE"QqqqbbbRRRnn݅ dƝw Y HMM \?vww/]aH*ظgϞX7+((ݻ7==AEEEGرc=<<.\?CTYYY__ǂ07 WRR2,,hϞ=40 HYY٣GlG YjIJJ)S8쎎g?~\QQ1)))&& 6lXGsrr߿/PP}l|x11Xbγg'z' PQQQ]]]}͚5"==80ljjz՟ n¨j0 KII͘1ϟ?Eݻ7vطonڴ AO?:u*88xŊ0{ŋe˖kkk,\֭[Bz޼y׿?r䈧ɓ'> ð={^+VDDDL6mHܹsǏg?A%K<|PRRDII [UU0ډHHH:unBmTUUUTTпٵk˗/uuu;m\fڹs֭[nݺ|r7770OC`^ Jr\.w˖-f̙3&HFIK߿wG@:::#;uܷo0f̘1ƷoҒorʢ"ܷpTpJ\ P(ܱcǿ/pAQe E ssseeepHKA^5 wY[[655?^,z,@2vB |^pa͚5B|xm۶Vy !_`2@,r7oހ @]LĢ‡Ȁ###GЀcDmmm)))}ݎ;uG555ↆ_~<00~Xo8paӧ)S>|8## ip";⒘*Q\*˗/'qPD [n}]uuu```oo޽{v-.+##o>gggSSSYYلK I!&Mŋ6Eڵ gϢ[YY 3+nnnjjj>>>cƌihhx111gXXA`Qaȑ6fEEQFm~ы/nݺ{-[^~}֬Y_Xv-::Bd``ݍIA邋44T`CeRs~1c(** 윜 YYqYXXxxxeff?~\FFfX@nN cǎ۱c:$V\ 8EGGgddpzzz֯_ha-A!. pOq H!LB 0w޽pkkk,X --r@t7{l[[ÇڵKSSS$- D"ѣGM&Qccc`ӽ{,Yw߁)WQQQWW>y z{{W^ V]]]-[6rȦYfꦧlJCA\\\V^pItȩSN0-,, }||n޼ k׮cS4O{{{---3J!+&QOb* ;A+VڻwokkٳB%Kz1cFqqƍihGX˞(0sى'>|8** ;ر}eeeXTqٞX ;w. U}DEhkKTAʀ bl(,_~#+WM6l0t 1Bt֭)Sϟqƛ7o,[,;;ӧOPYFNNѣG &&&>>>7nܸqFww\TTǏbqOO0!!!%%L/˖-{{{B!S__LHII p\U=///## A/q4pHTIL3 X, ֭KKK|N,!uuuuww_ ,-- kGGG Y^^L8?/\L 0 FݽJGG7ڼdɒݻwpX,VTTܸq#6..]ݻqݼyׯ_ϟ6I}UtIHH ƎG,2{D.)UkT67eqTl+)'"t`޺uի544:m۶3gfss3QrkkW`` JJJ;wa$" aCCCwwwLq8 (<<<11O,aCg܀"jڴiwy&7/ ݻwDE%%%---aXZZY:{]vիq`^l*s4lX悂P(! Jņ\0 z˃u0۵k׀& 'V,o޼yහK"k޽kddʪgdd{(1?&`*A?0""UB|rB~l t }qssn\jmm 1cFMM z ~ǻwr'NNȄ .]BOո}yLH[[{١?.ddd >Dz555I7x ]2mڴi6lԩS/^!Bz]Ozn___yy}Cޮk.❊._j`` p$!!ZZ.&''744D!$1#dR)c tVV\vv6A}}}6W7zDɈ  pKKKGGz5 /P򾾾IcX 6׭[ ~X{޸q4X94ZXz{{ۣi3yd)))''#G#כ>'\0e&yFNd۷o?~|||OA8S6lpedd\]]mllP>aÆGi҈ IXG)//R%0 NOO=XF#r`/ k֬~BBX^ {ϳ1^Ἄ---m@)i@T4ir!)Sb`` C 3 !DjHߘDW^EСCӧO3gH auusbv` 4Tm: `cee铛%X@M81##_Z:3<O^^Ap(:N'R墫T^YYիE"6X;XBMHh0pݴiLDFFҀhbwڵ^vbƌ_H$2006gHJJj.]ټyI&UUU׳XI&Ar333-,,ׯ_P9Z8&Vw@TiF̙3eddXopLTGӷFR"ܙ2Nх ǍN䦧pmb3 R8! *ttt~ݍ] XPCN*ARRRBPAA`X]]]ըܮ.w"b@pT ۷755tvv~Jq%^*% NFc 2UbhQ NNNrrrh!TPPhll,))rCD^q)EBT`URRIj9DX @IF433KOOWVV}}}FFFǏ͝9s&p}:Q4@3|ts2R!@[[[g("5cp =bW·ND9vvv\.B*oT^nh Dnp=Sg2#~=F*_ZZpp a$Ď`KꈍC7ojjj, +*8i 12̥|4ᕕ/_嫬LHHXp7o444I}:jpiYIo BsT GE'y即XPqjkkXJQU DV@,K RTҰ6g3=@p)/b000PRRɓ20 ?ql4uU>b Ѣ>UĬKW1(bJl 0 HhX'''gggmIII222EEE3f̐%H4+$c 5N&UT vH(@pvO𞝝-''^ %s(<Y!k;p[cc#1,*$6z%( =vn9C6q*!www_OqaT{+J#o uŝ / Z0һhDkdb?iIV n6 b{Ca?M1iMMof\G:ĤNb%1qC%? !bTօIb{R~4`b-33/@KyZ!Hck*{uwLEZNZN0fRbU4H]*@y@MA#K> N= cOG , <0H%mImúcc 4RXi7 bhS&];/To”sRI{DqHEBRY4O755EDDpwޖ-[H"|/0USGB%}'DNK/B.T ߿,**z8CoW 1d M% ~v >EhذaqqqOmVZu%ļh S캺|p+pr tj]$넸v 𗺡!L=Cb?h4$---|~{{"nhhP-x9_,6Lb߱86bwg.)3}(&ҨDPW zR 4zP ##3{쒒kkkx-R@~XYI:@I+D0K DDĜGC 1*yAe_)@D*T0 [ZZf_41/HfPI&"}A$cBlL B '1H%vpEՅUD0#iFdOC!@bAyz6@>kTi}p jRhbV:@ʿ(p*'ҰOܯ}( X8uhՈu rHJXEvgUK?HQB mI ꘧/*CF1FKT)~:@z NZBDIUFn3.;1Uh1ISk\2hȡBRU45'V PZZfq. i4kp*zXG&*rdo 1!BQ_8E+F)--mhhZd A_ʦ|%ȼ+^"%Qq~/Q``` (!!\.744g;;;OuttN2|TVVN8|677s80yAPcccmmlhhr&L ޽{goo>೷Ç(KzzzYuuu塗 pʔ)G%р(//qmЉ8T|8qgϞ?l6;!!a߾} `RRRjj`xMzz]ׯsrr~gŋBoooVA]]]i&Y^^w9YRRrӧOO>ݼy\>AP~~ݻw?>sss>|x𙕕va𙑑Ӄ޽_ojj* f7oԯz5r:[WSSsq\UUui???6pT\\}SA ?{d+K KEe ~!Ԇ1!D;VKGGGVV?**BlN>|/jjjHa?cbb"$''УC***֧"lrG0 D"p). pB1 Ԥ&))}}}X,z9&yK( Y,vG^^ennnU;Cq%b1: .`F ýiSM0I߉HRI7K0 XR"DD̢(oʐpv_t1544=[PQ < ^B͛7nj#)IrTܵkRSSuuu&P痗geeE"000++ܜh Ï= H,Y \͛YYYs7oLOOWRR"4!ao߾!?~811‚xB0 >PQ(@\QDh#ߞ W-'"#P$e077///|2Ꞟ0|@ҵ7nmii? lܸիW8---xyy}gOKK )mmm}}}<>{ kjj666p߾}+ TlXZ[[֯_?|t@baa!80 }mmme7&&ۤ>bbb Ab Ld0, /]v,&X,ʒ}NZYYoܸ蛖}2kkkAo߾]f2zJ=4iQh<X~~~'Nܿ?1 VXaiiIxG1}touu9sO|Ic\(`|#H#*_`scc/R[[KwjRf__ߪ*R_~ʷe߾}!bqUUՙ3g#~%%%uuuWQQ)oFboDD~Px &‹?~ȄgAAAB0999==Ç2 __/^&M@@b" _A#q!0 Y?>MMMwojjjmm_z۷okkkwuuԘa}[[[tuu8ďRhhȑ# fdd 1b1722R__566!}䉎DgϞihh6l0fpll 8|ĉh ^^x,---Jy򥢢 Ϸ|o"RVVVss6Ø0z]]]>?}tzuUUUcǎcqqqaaY~8͵-//GL^M;.6jԨÇ+**Knnnwuu-**;w.3,0!! +**mب|P(,++۶m[SS[[[!˽o]]]ll, )++nnnF0ۛfa</))PNNnݺuD_VVVvÆ DԼJJJzzz}ٺu+W  ۷}߾}ѽ  >|E"Qffffffmm-bqfffFFFUUձc8&݃AÜSN˜\VVƄǏIII@rY~~7o ϝ;G"z >o^zu{{;vqqGyVZ֦1o<===788xժUNNN ,*900~jjjNNNذ<|0** \Ћ?~hѢ6A3XpaWWB~qTT… |>XE/X烫q6o=GQϟʆVYY믿º%%%߯WGs|||Ri޽/^ Ak544444KCClD{D"D"SyyܹsѾ^dׯK=z3Q|8(d2WCCVee%~n2 ܹsgMM͊3grT(>>>bss) wCCCN455ЧLFN+$%%,))䬨ˉ4R͛7ඡX,FR(411G._xU鄄+W@ɨ^a.] KSSS^P(.\MOO|BzQD鞞 8"e~YfdIIɱcd2م  JeeeR,fRa*++>,HXq>z(Ljkkb1Lt;vX}}ŋŇt̒ D"cިͫdb7o\]]qqqnnn<*MHHpuu}-Ǐnnn\)))<ۆɮ111үI{撒77P!̥{1nnnB$WVV߿?((HsMM;ώ%M s}}a:3 iWh*\\.G_cQBPr\]]ZE5^h&> foQ@s}}W& 'J͓N2[k2woW0WѺJؤ ]_*/VlKN/^=<L{$$d7kZCXlRG>n.yzuXQiPHA{ y&Ug:JCAdw!Y D~VjEDX'C *R (@Xʒ@DIP-D]*<K1oVZТ^šůP("o) yZB3WuqqA>:LT(Ώ2uY999>}j׮:ȉ(*!!ϕAAA:t(--iӦ EQ ݸxMMͷo߲|}}۴i2SRR^|YWWwMkk뀀j333XW^^nff+455ԀFH;pbx+bKpCN.uGL)Ҥ$MMMmllﯪ ӋܹBPUUDo߾---MNN֭`EE!(JMM%`oܸQZZjnn7طMJJ233S սq7RլYG) ֭(ĉ/_(jʔ)rZSSsȐ!!!!Gzeiii۶mK+ǏSRR9>֡C+V444o}֖amm],Y$??K.:thhhi:##o Bt4jE\رӧOE-_>>>EYYYM4)"">;;{„ {~}׮]Ԇ ٥K7oL0^ -ddd4rH)ڵA355 ر#xӧOcǎ8-UIXmmmBP(*** 3D%%%&&&%z?YZZ֪ihho&%%Ox._|֬YE9s&--mY0uuϟ?[XXdggI$]]]ERSS# MӽzQWW#_~fxUt*ў 6b>\TTԩS'www۷ow3f%H$rcccLE8iҤp[3%%҂{geeLFFFsrrX}Fù O2i5X,.((`=SUUU__&Hdff6D^MP(9$33S&Ձ*++ \0LbbbqqD"JsrrcFe-'\+J\^-(ɞ?ZV(2,::\MT0̇%[JV l{D7kF_Z Eir8Ѡef+ <"!ҝ REBouq2>o&3{ʐYDd&g 9x>rņfMUD a)ǙD,_Aڋ_ HUg"@f&KW(᫑?y}arrr޽;gΜ`'''2RE+%|R&'޼y3b~o T*MIIb?~hiiEأG---d2YYYT*( <+ kff"@^zGCCCCCaÆBRݻ (dUMM zLbJJJ^*++CCC)ڵґ& m TWWOjhh<<(X٧O(Qn߾-utt f``P(ܥK2dSf̘۫W5k߿")) H~zppٳ}˗/G]\\L{e˖544PUZZ r;~իٳvZ92cƌe˖{ T|9h͛nii`CCC|!h۷o?ݻw_~D"Ylӧ}||]&&OrHEEŖ-[V^.HTUU/^?4 ^8~8<77777FT\.?qD@@m6pEQZZZIIIp($$$77R,ߺukxx88(SSSp,0̬Y~L ===pQ -[6iZ*C|UPd"*X| xRL&0744 H000 + X !S.r4|ƍ004I˫Xx $lz!ʂ9rDl,5y.,^pd(~:YP%Q rTe(h!SSSo'$$H/E&dK.[,$V^g񨨨888x(Lrd4̄,fˁmC<,hHh( lT)&~ӧϾ}z?͛7/''ujؽ{:|zLFl<eEKVl,ݻsΚ5k[WW'X} &I-At >45KC-xb0#iLB?~JB"L'YU,<ypJ4O#%f)B(u`3bi 8@V6x|F~D։@J[!p Na@ ?#@~g}!h9䑀>7T|M\Ol+6 CQPBfTQ@o2/,ĝ4.[ )Nyhpbٌ& ^4{)b)]Fj lm@wuXe|=Uگj8 B*TWȵR"R3Ѽ<⫼:w;QR<OR&٬o |bїȅ_)!k P *7A"2'|D5 {whϟ_~mjj$$|9Ґj4 {~t^ߖ76Uȣ&d /))yU6m^x*((E!*kI D&hh:w uyrVŋ,##A&[`KYO4x)NWW> y& Dsx8GD$ܼf8Dd.}TWW3&33pΝ;ѣGh,֥f+%z8Üoߪw˗zEcǎ( .+Y|.!<Js6[%j 45ӧ}B*Usxh!qud'H ҁRxla@.;MxEX‰(3yDǢv ۏs2 L3+=N+$ϊ)*E2hUr~\qD\v_[n={v,8Z.{pˉpN`= e+r"V*7jDT @TH *mAI6"Xb *\h.khʋ/b1@rڴiÇO6mĈg|uG$u2wiȐ!Em@"_׿gF\呸h^I\pA#@cpxl>"`qq{ij./r)ZM6?~m6"L"ܟ\qøT4 acanݺUVVVTT o~Z=kbMxѡn``бcGVF4} ^t K@ww,.*vC$UUUMl@0?(. KYUUK.\vreyUWWsT!744U(2 dFEE]x7Ǐoii [7n7|m۶7zxx/L{yymݺmL` %+RDĉ+{YYYR.k}28::3g( }mppׯqf X풒8V'"u P\}}}?nccs/fHNdPhAbݻw_vMÇ/_|ȑ,*ŷsoЋD"T>nܸcff6p@cWsO矫W_Ƃr<33W;v,8ի񇛛ϟe!Muuu;wLLL,// "667׿"##64FF9h uDMdHtӧs꒓_~ |9`.///((3H0 ӡC_~Az_lj'~iݺu|g*O VjZ6lQ %J.]>}~zPHڂ N:եKI&ݻw͛7E|ɓ'.\pttDmv ~aaյ>|ZSScǎaÆꪨXXXtuɒ%4MxÇ{쩮{ҥKZBձKQTqqX,.--?|p~~~~~aeeezz9sݿH[KKˈkkk$sٳg(́+!˻v\]]͛wΝ#Fl߾}#Fk]yyҥKMMMY;w=zllܸqʕ)S<==iQ(-s`` n!Mb̋^bcΝ/rbboݺ;A*XbȐ! 0f95S4]]]^|{npJkfKJJ2d3I`d`Kxx8XI{Ղ4M+#Gºeee<رcM߾}׬Y啙I'MtE3X+Ǐ/^xc O:\ak׮mڴÇ̬ ytvv? "LVT*=t萡E &D,!ϟOrrҥKǏfs[n_M0رc~~~QQQ>}BgHEEEFrss6mڡC-᲍tuu]hԩS\B9"X7h>!....,,СTH2##\oaH7lz !>>~ƌ:::NNNRT__lI1==\xLJ0˗A]9s eeeVھ}sFOQTCCC۶mUUUa;`4xeȑeee`ÇN1cFvvX,޷ouV4CeԩvvvYYYuuuVڹs)Sd]vڵ3sW(ׯ3DfffR55~m„ ֭3g?ЫW/aÆ Θ1ʊKݻw%ɑ#GGzL&}jjjsmӦMvv ba.]LMMtrʕݭ[6lpqGryJJJ۶m͉())qww?{l@}vjhhXb߾}VB[^ igϞHRD{UV{xxzk>}ZWWdɒ+W$&&J|rvv6EQ1bĔ)SfΜP(6l؀ (77ѯ򐐐{pDi… .izܹO>uttjkkf 0qqqG8p:iHP(޽d===cǎ_zzz;vd555m۶mhh믿***޼y]cMMM\ѹs:dll秭k.z~~~NNN+w޹snܸeҤIΝ qvv7n̙3mll?\PP_PP 0́]ݫP(?ֺ#F|w b+WF\.///OJJr~駞={z*''g={422g4SvFGGGEEƾ~nrpp(**ZzuϞ=ׯ_3F@~ǎnj^@YaҔBZ'O.**oVXq޽{1EQE8p`۶mpʔ)࿁L&x`ooocc}mhh޶mEQg655={,fmmݽ{w}}ϟOHH066NMMuց:wLlB" >\SS<γI[[gϞ--- qL&d'Ox"bii9o< AǏ>}6l7oBCC p'N !hhhRծ]_>xذaf***2e SUU4i9x˙0jۻw/EEE;w ƣdzfΝ;2+Wnݺ5--mԨQgΜy_sɦ{qq?/8::Ra8sLpƍ͛7?sk׮1cp† jkkrٳgsrrh>dE4_TTtIu twÇ`_yi@t۷o_W֭`wo߾E<`wtgϞTXX8rH Hҋ/Ax񢲲rӦM^\tӧ?}(soV__Aڵd?C``ի:zh/]y۷LUڢćQц_W1i??cǎ>|eygիW;wb'd D*޽4hPtt4*䧟~f;;{׮]\+:i~򥛛*xˡ4i&رuss?~|jj]hh(:ivڵklll@<|0F YjUUUwra/0Ϝ9Va`j =vBɃѣGv݅zi# K޽{CO&Lgy?Hs쪫Y>}:MRt߾}`xvww?tPXXwwwwwp_[[  o޼!7nҥK`'%%m߾}@wqH$JKK{9K.!J555ϝ;ӧڵk 555MMMѶmCT ޽{?,,,;ӧONNN ;wҌP]i-zeeWX<*Z;ZG6mZ\\%KΟ?$J3'>,\pΝ.]9gϞ󋍍ķ(y6fmmmೣ ܼyǏgtpp- ??C>}wfϞ~6zbbbΝ555㏄mrCCCX0LPP[ׯ_KWX &$$DFF-ZegBBxaٲe .yE߿իW,a^xqʈ9{lCCCRj[H#W-777LV]]}/h3"11ʕ+Rb'Q*xąKRad2lْ߾}QSSdɒGȧi:22ի-*ŭ[&O ƕ޲e~7pYKK/))IMMݽ{wMMͅ lmm^ Z~='AtVV[?D ĀtܙLg߿ Ϟ= |۷+**`E|c937Pjq ģ>C*VUUV[8qU' Äz{{Q - xE- ???06s[\gddݻ7""bԩXJ8$o۶СC;vzxxddd\r|A}7o,//|B|!CfΜ fiv޼y+8\~x:o߾ N}B9/mÆj'Bbq`hUK!O])J| [6@6Jӆ?ϪW)<*"(***++K./gϞ1L7@u ##{P<)srrrڴi3Ə?~x,,,ڴi3w\hvuu.++H$+V(_EQ֭ )(((*,,L&୪?~<8 4" NΞ=;00088رcb^^f]]XP3fmbbO?^ri@qmڴ/))ᑢt2 ƣBEEEGGܹs7nErrrEAA2Kfoݴif+?^QQQ__O,mӦa|XSScbbNt SSӐ"RQk7YETBP iyd6$OHtdd E!<Ҹ^=.....nk׮&NQD5uԔ'O&y@%yȑhɉӊ&'44tƍBdB8T__Ƕm*} y(x2 SYY1>b F`_׀D"ъ+rMaaUN<9|σ07 ,<@Q?#""^zաCp7B`c("(a 踺x,H$=ztZZZ8?a45066כ7o_233 BN\ULnҡpB$&WAY՛*e;y*ݢ|˖-#ߔ_79,\E޼ynHtXWWWZZ z$q^l6@iv툈P͌sa֬YEqE/ ЇH5q0:5i z(☘:tŋbf-<8?Noߎۃe6$P4h8$̙'$2s*kUFk*q ah|K?*Q/ڛ ` ]_$ߢrBfC*ov?kvH47fZKn4`lW!+|NWơώC5| \L+6)g*5o(T|m6 q2J P8 ]uIFHSd*@3ƥ '_W)%ϏXE=Tu6 R[g.w}yt)Exg/]DJYlJ5s "F4x5rh|4(ӧWɰ$=zo(*;;ٳ qE777LKKv<,99$?~k.LHHwގ;@2..Ç[n7o<}i&" gg?$KKK{i,**:xɓ'AѣǏɼ<#GxaÆҿ;wۗ(TP(%G"&D$ ޽H$255U:t4p~:d ihh0_UUzzzPյkW}}}Xjnn޾}{433366ISSSSSSܹs׮]aĤ{0illleeFFF0ٱcG&bݻa{;wnݺiiie6m`R&Ġi[[[p^EQ+VxOLNN֭OgBÇVVV ١C"wYGGGsjj@|0''U^԰A'Oy7nP(:44|2f nī(e+( oۛwMT@DPp7~neee4M]&.]$ɖ?|AեRiBB­[yCTY???w_ZLfbb(qw>} py֭7oº۷oJOO2df== tAG|8o)))?6660a<0Һ'0GP(ݻ1cX-,,hի#( ZZZݺuן0aZSSW^999cǎ a^~ݧO5Fμyka޾}뛐n``㓐֣GuuuzRRcccvGssٳg 4ɞlff6k֬wӧO711:99522׿{)KojjQ۶mܹӯ_?hkaRRR@MfO\JQTFF}MM.6m<}TWW_rvvq ۷oϜ[UUծ](1PPPо}-[tرSNE"ѬYJJJ8"PEQ"D&ijj+**̙#b=a7o]6:: Jbcc ֭[ٳiӦAű9997n >}:[QQQ\\iӦPһwJJJLLL[WRRҡCR<_VVgkkGhCE}.KKK555p.&iiZMKC(uLm:8bE[+-$ aHWI)lB|@&nw[!ss~{]ðSNipM!ٖBP:>}t*"6`gRJ(r:; Tze G$ ;/~ۻ.Z,L&SRR rfロK Y9\:́@n~ 0k QuQPH :|@ G ?Z_A]>O@'&&n޼f|>m[V @0{_~AZX,@͛Lc5_A499]V;77BDvGF1u:`|LꮀDZ yh4j5jzǬٱ`0j@R;wP%/..޺u+ zșX,###h4Jdj af4A!>qF86LyR5?_~P(A~snr@ ilL&H$*I=p@iiÇkjjhT.+*++  w7xZXL"={JtHD*:W_}!ɐPH*\^z;Nzð2{^ɡ2==hyy;v{G^xTz3n 0X8^`bnnf>X,KR__gϞO?4ўI={ gmllhY^^6L |>jE"jLz>oh,̨T*PhX>H$RZZѣGuuu"l6.X[[tJr߾}_~%J4P_=i7H6z(0 uLl EQA[[[Ѩj=t֝;wX,T*r_nll$@ YQQad2+@h4DB&-//:u PV UUU寿:H SSSHl6KӧOT*`*}9sFTl@qݵRܹsG%Gܜ鬫3 bte~O <:Y__7LgΜj{mkkdLgjjܹs4fJ j---p8.\ʪR8}j5JLc(`r.\(--W|։ .!z-F^|>L_699p:::\.y0Ht3i:QFD0\__L%u;v_5U*41 HT>P(0 &@ E0>Ą\.B=== q3'YTիYL>LfիWrIP]H$Rkx.@,J-;<Eѕxh0,))O& d28qBWWWkH$ rmm-ut:Q~:9D(&5>o2V* сX,٬T*Zd9NѸQQQa4x(G,aRyy"IvY&nۍu\(nooOOOx~!N+fWVVn7 6nb1HqF{{(\y-}`Fs!L&S4'1bti*rx|&b##woi)oW\I$Lȑ#SSS rx<6x<p8LA&L&}wFIqʕCTZZz嵵T*l^d {a' o߾~X,lb8 BESuhT.R! 7`q]oooKK_ AБ#G>x<.Hn\.w:LF$nDbdfEu}q<8pVNA^n5D nnnbM8]+`uuO}MMMx\i<ω}"P','\',xv0e)8*|U3ZDeVaVϓc*"!%mǐt.gI}bSJɪCf>Ki蜕4> endobj 290 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 291 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 292 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 291 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 294 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 293 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 46375>> stream xw\:s" h&**6MFcQ+Xb* M@:ʡr?q޻Z{aA  >a s  kiX'O%狓F B@Du8R "&1τ pC? NP50PL!ڊob1)%Ep.(fmrz E#C@; }LGA,RW_`@X* MMM111Z . ϺW^wsݽ{±C^XXFjғ'O^DDDtvvill,..NMME㭸ĉ… @ ۷o &j|ww???Rh`;77 APUUՆ <==;dRzuMM =O`` x<X֧O(gjj* [nv 4'` iii$"f@/_looyNN=H$*--===EEE ?666EEEmmmUUU\.7;;677\^\\\YYp,Y t.\|8q j^mmmcccNNP(c{{;`Ņbᔔ eeeEEE{|>ӦMaaa---eee ;;ʾ⚚WWWWVV>}r X,tϿwׯQ;7m^BCC=z^pA$1I;4rRSS{zzHyZZZ|}}J"""b۶m 'tttݻw֬Yb8++ ׿Y[[T\\immQ\__ۛ';;;7n{nʕ lAAP(<&;;[ UVV655.bܗ/_bK,߽{7!!A,{zzbs'%%b^СC?-mmmffffff0 gff>xXAAPRRRoooKKKUU۷UUU_>iҤիW+((ܹsgƌ׮]p8Ϟ=3008x RRRzqGGիWӧ744ڵK$?>22l̘1Tkk)SD]v>wΝ𦦦//^xӧOv횲rBBBwwwdddyyyuu50233S,ϙ3G(khh\r$001c|}}>|8f̘@ ݻwǏeJJJlllyyP(|w}[^^󛚚>|YYYkk>dggGGGrܾ>]]]l%\A+HT__?}#F0TTT4vXw}6]UU H__ݻw%$$@IHH())illdX޽kmm}Ʌ ,XpB.n>[nuww'''C?{l@0u>,XYYYÇoooWWW3gTTT^|8nܸӧO;888pb=|nϞ=|>?**jӦM0 &&&Ι3SKK#$$#**իbܹsb8..֭[<ŋB˗>v옇 ÷o^hԭ[ VUUZ\\ jޏ?_999ֺAAA$cYӈ:666pN (//t)b^WWp8H$>pĉMLLl윙&..NYYyÆ JJJ ҥKl٢a==ްW^-[̙3g '..޹sd>,@wwwooouuu/CCCCCӧOo޼ѣGՅϞ=_wJKKO8q޼y999ظeeeoo UUխ[{n޼+)))p\. >}fŧO믿nݺA^vv6`>aP(##.zzzA믿JKKњ$\{{ڵk<<< ڲejjj>| Aɓ:33 6zxxxxxfggƊ+o^\\lcc3yd---444P,RCC3iҤuihh˗/=|||-[^QQݻ[[M6YZZ6qƍ1bȑ+W4irYY* 777//jeҥ[nJKK[`-[F p8rrr0 xx SSS?~|a ?S(0aF|Hbq(M6|߿cƌ5kֳgψ#Gyyyohhuuu&L> ><66 ܸW,Q[[~Ĉ+V~}RRR}}=8l-]r/((;FZGGGmm-pEfϞ=c 𮢢r~kّgUH$RTT( ())ill,//Y\\}zbbb[[ZA$V &L\SSmѵ1\BX[[\R ٳ(PVV߿뛜cPX__;wؘn۶袧[>\4 3gfaaqΝY (++P' 1XG"L9rDRR ggڣG>b8p`FFF;w433 HVV@KKKQQA333A%%%W^gmmm ?p̙_QTT677711 hׯ_wފ+P,0 Z޿laay̙>OOM6>`̚5Jeeel61dII'A/666 (66ӧ;wTVV>w\hhl$@QFIJJ9sΝ;(@333#F`***y&;;[UUuӦMUUU6TTTtuu{{{㏠ yy#GܹNKKkcƌAnn.vN477622@!q}}}Y,8rȖMMM` AΝ;O>moog[[[---_G(&I={HŽ_JD*eh*AH/ɓ'O|=p~kk+=TF^re@P? 4s8Xi7 CN H^^yqc"? ht~tGqiOELac&iP9BP 흘jjj \/^ |?~T*pd0; M!s ǎ+w 555LBD͇s}_@( |s7 yysb]ttt|"֥SNNN__?-- ,@[c3'e* ƚA RQX_ %HUPMMM:u*oiiQVVhRTKQHώʒ={"X A)4}37-l?TB2X~R_~a1z\XZZZs-))AaIa˅IL-&M4iDA4ʋ8:@uB/ijjbRlʱ/4^C44ZlyqHS A*sڙ@ QPPtbJb&/ ;::Μ9cllP @Iփ ]hI"%-[ikGLURR7nܜ9sJ(Iڼb^ݺuLJ8PUp]*~қP" 399ӧO֭B(%%=~ף/MO!SwKKK 'B/d.ao3(C9P}mڴI 0f<4Dt60-p '[ZZX9h`IRM@ WWWjhh(**% K߱d>;*W(n߾]$>__hc;w2!!aP~FBlzp8+###IKKKIIGωh i9Q"'68֗vKc?(q8n|Gq>O-4I OHr| Я@b1mkk#IS55,,L$uww  ɟ_7o=~i ˕Ϟ=prrO4?A#8C ('/* ,?@3Uvu5 &L055E{P N2.s`bc^^ÉC61D522r߾}?BKR ƾl{@@q㢣 Icjc0|U_zN @3A08&11x=x=weddo޼ήŋ߯)¹m 3(@yM6Si$3p&&&T_{wX N'0 5''66`kk֭[siiiݻwڵk^^^>>>aaaIe^AbXFF_n]FF1&aMMM--- Ͽq֭[5GHSSS(jhh|RFF8aptt3fL||˗,XٙAPaaa^^:R聅qqqAAA}}}ʙX; Zh< zڵkDBD9y 0̣TMMM^^~̘18wHtM;;{{:;;ԈÇ?}ڵk~~~4Aqqq!!!ǎCO:o߾֭[ϟ?/++[dI}}dTWW A.ёx{ sbX***3fZ O  }}}===(3KHH޿?a ooo6]^^cǎѣG߽{Ν;aaa{=qzP/zX,>qDrr}}}̙cdd48 ȑ#߿A gΜqqq9v옣#C;QbRPPaٹ,x0l ˗/;::0'5kzxxVUU _ZZZYY)$@RRRT j+ B ;uTWW /O+Wvvv/Ǝ{ٸ8P`LӧO&&&^zuxx;rssΞ=J7oz{{{{{++qϚ5KBB EFF5*$$ŋ'N;wٳgwU[[y…%KԌ7nxƍ+W߼ySZZ gp܌e˖:u*T[[[WWWddgΜyرG2:z:tԩS:;;###Q/ggOx<\@z( ***֭{!4\777'''pXpppEEEWWڵk\bXCCLxϟ?&~f 8@߭@رc,ٳGfA'O$&&7oӑyyy1}t *Zv֪U G3C@eG]v˖-gϞ p8݋Yl(x?x`)))G1cƂ pk---'N`8ohh8g___l!''qժU'N@4551bċ/hĒ ="""//_~zA^X 300={vAAV~VVrA,2VUU%!!!!!M))ƍgll r ~bϞ=Ν )++;r0m4;7U>#fҸ@'uX ȑ#AVx͛7n())i``իAdmmmddfo߾}%===vqoo\kׂܩ& .,k…L}i.O>UTT8qbNN88 999o޼ (<<ٳg6mڱcsYYiiiQ$UZ Sppƍo߾xb?.H+M:e(..>z7@PRR9r$8~by{{xxx\z?.^楉m |V,WVV.Va7o?[ӦMC$O>v999 A vvv8ZEEaΨ.++3440Mܗ2228{{{nƍW^ݹs ***|}};::]O?:t@ѣ 8).yO>500Xj6 E(]am,ӧQuQQQK,X|իW9Ϸ% L;0@ ݻ...`144455?>N 1/aߞK.Ca6Ǐի{.]ˡMMM_z5 jii}'/̙3411aXK.^W^駟gVVӧE"Ҋ+Νp !5b`cMM 8D okk< /^hoo ѣG .?Ic^__H$ZjUoo ].ꀳlvhh#G:::ɓ'%%%0D T> .QF KHcDRR9M\ك?_%RWWsΕ+W&Oc///p7)Q$Jsvvᅰ߿4l Ǣƾ@m۶5k֠?smmm _xqׯ_+xYYY5 |)**m莌,))9qč7̙UUUURR`dd?Ԁ*++kjjq\SQQQCCïJUcMOOkhhx" î౳bʣjuQA޿2cƌaÆ5779r9::n޼y0 =,444̜9055LGG?~<5[ UUU5l00c&Q9yd]]ݚ5kt.Q i}kCBB֬Yz˖-^tiLL̙3 @_N,pARRϜ9=r ښ6rH p777*;Iʕ+vpb16zE"QqqqbbbRRRnn݅ dƝw Y HMM \?vww/]aH*ظgϞX7+((ݻ7==AEEEGرc=<<.\?CTYYY__ǂ07 WRR2,,hϞ=40 HYY٣GlG YjIJJ)S8쎎g?~\QQ1)))&& 6lXGsrr߿/PP}l|x11Xbγg'z' PQQQ]]]}͚5"==80ljjz՟ n¨j0 KII͘1ϟ?Eݻ7vطonڴ AO?:u*88xŊ0{ŋe˖kkk,\֭[Bz޼y׿?r䈧ɓ'> ð={^+VDDDL6mHܹsǏg?A%K<|PRRDII [UU0ډHHH:unBmTUUUTTпٵk˗/uuu;m\fڹs֭[nݺ|r7770OC`^ Jr\.w˖-f̙3&HFIK߿wG@:::#;uܷo0f̘1ƷoҒorʢ"ܷpTpJ\ P(ܱcǿ/pAQe E ssseeepHKA^5 wY[[655?^,z,@2vB |^pa͚5B|xm۶Vy !_`2@,r7oހ @]LĢ‡Ȁ###GЀcDmmm)))}ݎ;uG555ↆ_~<00~Xo8paӧ)S>|8## ip";⒘*Q\*˗/'qPD [n}]uuu```oo޽{v-.+##o>gggSSSYYلK I!&Mŋ6Eڵ gϢ[YY 3+nnnjjj>>>cƌihhx111gXXA`Qaȑ6fEEQFm~ы/nݺ{-[^~}֬Y_Xv-::Bd``ݍIA邋44T`CeRs~1c(** 윜 YYqYXXxxxeff?~\FFfX@nN cǎ۱c:$V\ 8EGGgddpzzz֯_ha-A!. pOq H!LB 0w޽pkkk,X --r@t7{l[[ÇڵKSSS$- D"ѣGM&Qccc`ӽ{,Yw߁)WQQQWW>y z{{W^ V]]]-[6rȦYfꦧlJCA\\\V^pItȩSN0-,, }||n޼ k׮cS4O{{{---3J!+&QOb* ;A+VڻwokkٳB%Kz1cFqqƍihGX˞(0sى'>|8** ;ر}eeeXTqٞX ;w. U}DEhkKTAʀ bl(,_~#+WM6l0t 1Bt֭)Sϟqƛ7o,[,;;ӧOPYFNNѣG &&&>>>7nܸqFww\TTǏbqOO0!!!%%L/˖-{{{B!S__LHII p\U=///## A/q4pHTIL3 X, ֭KKK|N,!uuuuww_ ,-- kGGG Y^^L8?/\L 0 FݽJGG7ڼdɒݻwpX,VTTܸq#6..]ݻqݼyׯ_ϟ6I}UtIHH ƎG,2{D.)UkT67eqTl+)'"t`޺uի544:m۶3gfss3QrkkW`` JJJ;wa$" aCCCwwwLq8 (<<<11O,aCg܀"jڴiwy&7/ ݻwDE%%%---aXZZY:{]vիq`^l*s4lX悂P(! Jņ\0 z˃u0۵k׀& 'V,o޼yහK"k޽kddʪgdd{(1?&`*A?0""UB|rB~l t }qssn\jmm 1cFMM z ~ǻwr'NNȄ .]BOո}yLH[[{١?.ddd >Dz555I7x ]2mڴi6lԩS/^!Bz]Ozn___yy}Cޮk.❊._j`` p$!!ZZ.&''744D!$1#dR)c tVV\vv6A}}}6W7zDɈ  pKKKGGz5 /P򾾾IcX 6׭[ ~X{޸q4X94ZXz{{ۣi3yd)))''#G#כ>'\0e&yFNd۷o?~|||OA8S6lpedd\]]mllP>aÆGi҈ IXG)//R%0 NOO=XF#r`/ k֬~BBX^ {ϳ1^Ἄ---m@)i@T4ir!)Sb`` C 3 !DjHߘDW^EСCӧO3gH auusbv` 4Tm: `cee铛%X@M81##_Z:3<O^^Ap(:N'R墫T^YYիE"6X;XBMHh0pݴiLDFFҀhbwڵ^vbƌ_H$2006gHJJj.]ټyI&UUU׳XI&Ar333-,,ׯ_P9Z8&Vw@TiF̙3eddXopLTGӷFR"ܙ2Nх ǍN䦧pmb3 R8! *ttt~ݍ] XPCN*ARRRBPAA`X]]]ըܮ.w"b@pT ۷755tvv~Jq%^*% NFc 2UbhQ NNNrrrh!TPPhll,))rCD^q)EBT`URRIj9DX @IF433KOOWVV}}}FFFǏ͝9s&p}:Q4@3|ts2R!@[[[g("5cp =bW·ND9vvv\.B*oT^nh Dnp=Sg2#~=F*_ZZpp a$Ď`KꈍC7ojjj, +*8i 12̥|4ᕕ/_嫬LHHXp7o444I}:jpiYIo BsT GE'y即XPqjkkXJQU DV@,K RTҰ6g3=@p)/b000PRRɓ20 ?ql4uU>b Ѣ>UĬKW1(bJl 0 HhX'''gggmIII222EEE3f̐%H4+$c 5N&UT vH(@pvO𞝝-''^ %s(<Y!k;p[cc#1,*$6z%( =vn9C6q*!www_OqaT{+J#o uŝ / Z0һhDkdb?iIV n6 b{Ca?M1iMMof\G:ĤNb%1qC%? !bTօIb{R~4`b-33/@KyZ!Hck*{uwLEZNZN0fRbU4H]*@y@MA#K> N= cOG , <0H%mImúcc 4RXi7 bhS&];/To”sRI{DqHEBRY4O755EDDpwޖ-[H"|/0USGB%}'DNK/B.T ߿,**z8CoW 1d M% ~v >EhذaqqqOmVZu%ļh S캺|p+pr tj]$넸v 𗺡!L=Cb?h4$---|~{{"nhhP-x9_,6Lb߱86bwg.)3}(&ҨDPW zR 4zP ##3{쒒kkkx-R@~XYI:@I+D0K DDĜGC 1*yAe_)@D*T0 [ZZf_41/HfPI&"}A$cBlL B '1H%vpEՅUD0#iFdOC!@bAyz6@>kTi}p jRhbV:@ʿ(p*'ҰOܯ}( X8uhՈu rHJXEvgUK?HQB mI ꘧/*CF1FKT)~:@z NZBDIUFn3.;1Uh1ISk\2hȡBRU45'V PZZfq. i4kp*zXG&*rdo 1!BQ_8E+F)--mhhZd A_ʦ|%ȼ+^"%Qq~/Q``` (!!\.744g;;;OuttN2|TVVN8|677s80yAPcccmmlhhr&L ޽{goo>೷Ç(KzzzYuuu塗 pʔ)G%р(//qmЉ8T|8qgϞ?l6;!!a߾} `RRRjj`xMzz]ׯsrr~gŋBoooVA]]]i&Y^^w9YRRrӧOO>ݼy\>AP~~ݻw?>sss>|x𙕕va𙑑Ӄ޽_ojj* f7oԯz5r:[WSSsq\UUui???6pT\\}SA ?{d+K KEe ~!Ԇ1!D;VKGGGVV?**BlN>|/jjjHa?cbb"$''УC***֧"lrG0 D"p). pB1 Ԥ&))}}}X,z9&yK( Y,vG^^ennnU;Cq%b1: .`F ýiSM0I߉HRI7K0 XR"DD̢(oʐpv_t1544=[PQ < ^B͛7nj#)IrTܵkRSSuuu&P痗geeE"000++ܜh Ï= H,Y \͛YYYs7oLOOWRR"4!ao߾!?~811‚xB0 >PQ(@\QDh#ߞ W-'"#P$e077///|2Ꞟ0|@ҵ7nmii? lܸիW8---xyy}gOKK )mmm}}}<>{ kjj666p߾}+ TlXZ[[֯_?|t@baa!80 }mmme7&&ۤ>bbb Ab Ld0, /]v,&X,ʒ}NZYYoܸ蛖}2kkkAo߾]f2zJ=4iQh<X~~~'Nܿ?1 VXaiiIxG1}touu9sO|Ic\(`|#H#*_`scc/R[[KwjRf__ߪ*R_~ʷe߾}!bqUUՙ3g#~%%%uuuWQQ)oFboDD~Px &‹?~ȄgAAAB0999==Ç2 __/^&M@@b" _A#q!0 Y?>MMMwojjjmm_z۷okkkwuuԘa}[[[tuu8ďRhhȑ# fdd 1b1722R__566!}䉎DgϞihh6l0fpll 8|ĉh ^^x,---Jy򥢢 Ϸ|o"RVVVss6Ø0z]]]>?}tzuUUUcǎcqqqaaY~8͵-//GL^M;.6jԨÇ+**Knnnwuu-**;w.3,0!! +**mب|P(,++۶m[SS[[[!˽o]]]ll, )++nnnF0ۛfa</))PNNnݺuD_VVVvÆ DԼJJJzzz}ٺu+W  ۷}߾}ѽ  >|E"Qffffffmm-bqfffFFFUUձc8&݃AÜSN˜\VVƄǏIII@rY~~7o ϝ;G"z >o^zu{{;vqqGyVZ֦1o<===788xժUNNN ,*900~jjjNNNذ<|0** \Ћ?~hѢ6A3XpaWWB~qTT… |>XE/X烫q6o=GQϟʆVYY믿º%%%߯WGs|||Ri޽/^ Ak544444KCClD{D"D"SyyܹsѾ^dׯK=z3Q|8(d2WCCVee%~n2 ܹsgMM͊3grT(>>>bss) wCCCN455ЧLFN+$%%,))䬨ˉ4R͛7ඡX,FR(411G._xU鄄+W@ɨ^a.] KSSS^P(.\MOO|BzQD鞞 8"e~YfdIIɱcd2م  JeeeR,fRa*++>,HXq>z(Ljkkb1Lt;vX}}ŋŇt̒ D"cިͫdb7o\]]qqqnnn<*MHHpuu}-Ǐnnn\)))<ۆɮ111үI{撒77P!̥{1nnnB$WVV߿?((HsMM;ώ%M s}}a:3 iWh*\\.G_cQBPr\]]ZE5^h&> foQ@s}}W& 'J͓N2[k2woW0WѺJؤ ]_*/VlKN/^=<L{$$d7kZCXlRG>n.yzuXQiPHA{ y&Ug:JCAdw!Y D~VjEDX'C *R (@Xʒ@DIP-D]*<K1oVZТ^šůP("o) yZB3WuqqA>:LT(Ώ2uY999>}j׮:ȉ(*!!ϕAAA:t(--iӦ EQ ݸxMMͷo߲|}}۴i2SRR^|YWWwMkk뀀j333XW^^nff+455ԀFH;pbx+bKpCN.uGL)Ҥ$MMMmllﯪ ӋܹBPUUDo߾---MNN֭`EE!(JMM%`oܸQZZjnn7طMJJ233S սq7RլYG) ֭(ĉ/_(jʔ)rZSSsȐ!!!!Gzeiii۶mK+ǏSRR9>֡C+V444o}֖amm],Y$??K.:thhhi:##o Bt4jE\رӧOE-_>>>EYYYM4)"">;;{„ {~}׮]Ԇ ٥K7oL0^ -ddd4rH)ڵA355 ر#xӧOcǎ8-UIXmmmBP(*** 3D%%%&&&%z?YZZ֪ihho&%%Ox._|֬YE9s&--mY0uuϟ?[XXdggI$]]]ERSS# MӽzQWW#_~fxUt*ў 6b>\TTԩS'www۷ow3f%H$rcccLE8iҤp[3%%҂{geeLFFFsrrX}Fù O2i5X,.((`=SUUU__&Hdff6D^MP(9$33S&Ձ*++ \0LbbbqqD"JsrrcFe-'\+J\^-(ɞ?ZV(2,::\MT0̇%[JV l{D7kF_Z Eir8Ѡef+ <"!ҝ REBouq2>o&3{ʐYDd&g 9x>rņfMUD a)ǙD,_Aڋ_ HUg"@f&KW(᫑?y}arrr޽;gΜ`'''2RE+%|R&'޼y3b~o T*MIIb?~hiiEأG---d2YYYT*( <+ kff"@^zGCCCCCaÆBRݻ (dUMM zLbJJJ^*++CCC)ڵґ& m TWWOjhh<<(X٧O(Qn߾-utt f``P(ܥK2dSf̘۫W5k߿")) H~zppٳ}˗/G]\\L{e˖544PUZZ r;~իٳvZ92cƌe˖{ T|9h͛nii`CCC|!h۷o?ݻw_~D"Ylӧ}||]&&OrHEEŖ-[V^.HTUU/^?4 ^8~8<77777FT\.?qD@@m6pEQZZZIIIp($$$77R,ߺukxx88(SSSp,0̬Y~L ===pQ -[6iZ*C|UPd"*X| xRL&0744 H000 + X !S.r4|ƍ004I˫Xx $lz!ʂ9rDl,5y.,^pd(~:YP%Q rTe(h!SSSo'$$H/E&dK.[,$V^g񨨨888x(Lrd4̄,fˁmC<,hHh( lT)&~ӧϾ}z?͛7/''ujؽ{:|zLFl<eEKVl,ݻsΚ5k[WW'X} &I-At >45KC-xb0#iLB?~JB"L'YU,<ypJ4O#%f)B(u`3bi 8@V6x|F~D։@J[!p Na@ ?#@~g}!h9䑀>7T|M\Ol+6 CQPBfTQ@o2/,ĝ4.[ )Nyhpbٌ& ^4{)b)]Fj lm@wuXe|=Uگj8 B*TWȵR"R3Ѽ<⫼:w;QR<OR&٬o |bїȅ_)!k P *7A"2'|D5 {whϟ_~mjj$$|9Ґj4 {~t^ߖ76Uȣ&d /))yU6m^x*((E!*kI D&hh:w uyrVŋ,##A&[`KYO4x)NWW> y& Dsx8GD$ܼf8Dd.}TWW3&33pΝ;ѣGh,֥f+%z8Üoߪw˗zEcǎ( .+Y|.!<Js6[%j 45ӧ}B*Usxh!qud'H ҁRxla@.;MxEX‰(3yDǢv ۏs2 L3+=N+$ϊ)*E2hUr~\qD\v_[n={v,8Z.{pˉpN`= e+r"V*7jDT @TH *mAI6"Xb *\h.khʋ/b1@rڴiÇO6mĈg|uG$u2wiȐ!Em@"_׿gF\呸h^I\pA#@cpxl>"`qq{ij./r)ZM6?~m6"L"ܟ\qøT4 acanݺUVVVTT o~Z=kbMxѡn``бcGVF4} ^t K@ww,.*vC$UUUMl@0?(. KYUUK.\vreyUWWsT!744U(2 dFEE]x7Ǐoii [7n7|m۶7zxx/L{yymݺmL` %+RDĉ+{YYYR.k}28::3g( }mppׯqf X풒8V'"u P\}}}?nccs/fHNdPhAbݻw_vMÇ/_|ȑ,*ŷsoЋD"T>nܸcff6p@cWsO矫W_Ƃr<33W;v,8ի񇛛ϟe!Muuu;wLLL,// "667׿"##64FF9h uDMdHtӧs꒓_~ |9`.///((3H0 ӡC_~Az_lj'~iݺu|g*O VjZ6lQ %J.]>}~zPHڂ N:եKI&ݻw͛7E|ɓ'.\pttDmv ~aaյ>|ZSScǎaÆꪨXXXtuɒ%4MxÇ{쩮{ҥKZBձKQTqqX,.--?|p~~~~~aeeezz9sݿH[KKˈkkk$sٳg(́+!˻v\]]͛wΝ#Fl߾}#Fk]yyҥKMMMY;w=zllܸqʕ)S<==iQ(-s`` n!Mb̋^bcΝ/rbboݺ;A*XbȐ! 0f95S4]]]^|{npJkfKJJ2d3I`d`Kxx8XI{Ղ4M+#Gºeee<رcM߾}׬Y啙I'MtE3X+Ǐ/^xc O:\ak׮mڴÇ̬ ytvv? "LVT*=t萡E &D,!ϟOrrҥKǏfs[n_M0رc~~~QQQ>}BgHEEEFrss6mڡC-᲍tuu]hԩS\B9"X7h>!....,,СTH2##\oaH7lz !>>~ƌ:::NNNRT__lI1==\xLJ0˗A]9s eeeVھ}sFOQTCCC۶mUUUa;`4xeȑeee`ÇN1cFvvX,޷ouV4CeԩvvvYYYuuuVڹs)Sd]vڵ3sW(ׯ3DfffR55~m„ ֭3g?ЫW/aÆ Θ1ʊKݻw%ɑ#GGzL&}jjjsmӦMvv ba.]LMMtrʕݭ[6lpqGryJJJ۶m͉())qww?{l@}vjhhXb߾}VB[^ igϞHRD{UV{xxzk>}ZWWdɒ+W$&&J|rvv6EQ1bĔ)SfΜP(6l؀ (77ѯ򐐐{pDi… .izܹO>uttjkkf 0qqqG8p:iHP(޽d===cǎ_zzz;vd555m۶mhh믿***޼y]cMMM\ѹs:dll秭k.z~~~NNN+w޹snܸeҤIΝ qvv7n̙3mll?\PP_PP 0́]ݫP(?ֺ#F|w b+WF\.///OJJr~駞={z*''g={422g4SvFGGGEEƾ~nrpp(**ZzuϞ=ׯ_3F@~ǎnj^@YaҔBZ'O.**oVXq޽{1EQE8p`۶mpʔ)࿁L&x`ooocc}mhh޶mEQg655={,fmmݽ{w}}ϟOHH066NMMuց:wLlB" >\SS<γI[[gϞ--- qL&d'Ox"bii9o< AǏ>}6l7oBCC p'N !hhhRծ]_>xذaf***2e SUU4i9x˙0jۻw/EEE;w ƣdzfΝ;2+Wnݺ5--mԨQgΜy_sɦ{qq?/8::Ra8sLpƍ͛7?sk׮1cp† jkkrٳgsrrh>dE4_TTtIu twÇ`_yi@t۷o_W֭`wo߾E<`wtgϞTXX8rH Hҋ/Ax񢲲rӦM^\tӧ?}(soV__Aڵd?C``ի:zh/]y۷LUڢćQц_W1i??cǎ>|eygիW;wb'd D*޽4hPtt4*䧟~f;;{׮]\+:i~򥛛*xˡ4i&رuss?~|jj]hh(:ivڵklll@<|0F YjUUUwra/0Ϝ9Va`j =vBɃѣGv݅zi# K޽{CO&Lgy?Hs쪫Y>}:MRt߾}`xvww?tPXXwwwwwp_[[  o޼!7nҥK`'%%m߾}@wqH$JKK{9K.!J555ϝ;ӧڵk 555MMMѶmCT ޽{?,,,;ӧONNN ;wҌP]i-zeeWX<*Z;ZG6mZ\\%KΟ?$J3'>,\pΝ.]9gϞ󋍍ķ(y6fmmmೣ ܼyǏgtpp- ??C>}wfϞ~6zbbbΝ555㏄mrCCCX0LPP[ׯ_KWX &$$DFF-ZegBBxaٲe .yE߿իW,a^xqʈ9{lCCCRj[H#W-777LV]]}/h3"11ʕ+Rb'Q*xąKRad2lْ߾}QSSdɒGȧi:22ի-*ŭ[&O ƕ޲e~7pYKK/))IMMݽ{wMMͅ lmm^ Z~='AtVV[?D ĀtܙLg߿ Ϟ= |۷+**`E|c937Pjq ģ>C*VUUV[8qU' Äz{{Q - xE- ???06s[\gddݻ7""bԩXJ8$o۶СC;vzxxddd\r|A}7o,//|B|!CfΜ fiv޼y+8\~x:o߾ N}B9/mÆj'Bbq`hUK!O])J| [6@6Jӆ?ϪW)<*"(***++K./gϞ1L7@u ##{P<)srrrڴi3Ə?~x,,,ڴi3w\hvuu.++H$+V(_EQ֭ )(((*,,L&୪?~<8 4" NΞ=;00088رcb^^f]]XP3fmbbO?^ri@qmڴ/))ᑢt2 ƣBEEEGGܹs7nErrrEAA2Kfoݴif+?^QQQ__O,mӦa|XSScbbNt SSӐ"RQk7YETBP iyd6$OHtdd E!<Ҹ^=.....nk׮&NQD5uԔ'O&y@%yȑhɉӊ&'44tƍBdB8T__Ƕm*} y(x2 SYY1>b F`_׀D"ъ+rMaaUN<9|σ07 ,<@Q?#""^zաCp7B`c("(a 踺x,H$=ztZZZ8?a45066כ7o_233 BN\ULnҡpB$&WAY՛*e;y*ݢ|˖-#ߔ_79,\E޼ynHtXWWWZZ z$q^l6@iv툈P͌sa֬YEqE/ ЇH5q0:5i z(☘:tŋbf-<8?Noߎۃe6$P4h8$̙'$2s*kUFk*q ah|K?*Q/ڛ ` ]_$ߢrBfC*ov?kvH47fZKn4`lW!+|NWơώC5| \L+6)g*5o(T|m6 q2J P8 ]uIFHSd*@3ƥ '_W)%ϏXE=Tu6 R[g.w}yt)Exg/]DJYlJ5s "F4x5rh|4(ӧWɰ$=zo(*;;ٳ qE777LKKv<,99$?~k.LHHwގ;@2..Ç[n7o<}i&" gg?$KKK{i,**:xɓ'AѣǏɼ<#GxaÆҿ;wۗ(TP(%G"&D$ ޽H$255U:t4p~:d ihh0_UUzzzPյkW}}}Xjnn޾}{433366ISSSSSSܹs׮]aĤ{0illleeFFF0ٱcG&bݻa{;wnݺiiie6m`R&Ġi[[[p^EQ+VxOLNN֭OgBÇVVV ١C"wYGGGsjj@|0''U^԰A'Oy7nP(:44|2f nī(e+( oۛwMT@DPp7~neee4M]&.]$ɖ?|AեRiBB­[yCTY???w_ZLfbb(qw>} py֭7oº۷oJOO2df== tAG|8o)))?6660a<0Һ'0GP(ݻ1cX-,,hի#( ZZZݺuן0aZSSW^999cǎ a^~ݧO5Fμyka޾}뛐n``㓐֣GuuuzRRcccvGssٳg 4ɞlff6k֬wӧO711:99522׿{)KojjQ۶mܹӯ_?hkaRRR@MfO\JQTFF}MM.6m<}TWW_rvvq ۷oϜ[UUծ](1PPPо}-[tرSNE"ѬYJJJ8"PEQ"D&ijj+**̙#b=a7o]6:: Jbcc ֭[ٳiӦAű9997n >}:[QQQ\\iӦPһwJJJLLL[WRRҡCR<_VVgkkGhCE}.KKK555p.&iiZMKC(uLm:8bE[+-$ aHWI)lB|@&nw[!ss~{]ðSNipM!ٖBP:>}t*"6`gRJ(r:; Tze G$ ;/~ۻ.Z,L&SRR rfロK Y9\:́@n~ 0k QuQPH :|@ G ?Z_A]>O@'&&n޼f|>m[V @0{_~AZX,@͛Lc5_A499]V;77BDvGF1u:`|LꮀDZ yh4j5jzǬٱ`0j@R;wP%/..޺u+ zșX,###h4Jdj af4A!>qF86LyR5?_~P(A~snr@ ilL&H$*I=p@iiÇkjjhT.+*++  w7xZXL"={JtHD*:W_}!ɐPH*\^z;Nzð2{^ɡ2==hyy;v{G^xTz3n 0X8^`bnnf>X,KR__gϞO?4ўI={ gmllhY^^6L |>jE"jLz>oh,̨T*PhX>H$RZZѣGuuu"l6.X[[tJr߾}_~%J4P_=i7H6z(0 uLl EQA[[[Ѩj=t֝;wX,T*r_nll$@ YQQad2+@h4DB&-//:u PV UUU寿:H SSSHl6KӧOT*`*}9sFTl@qݵRܹsG%Gܜ鬫3 bte~O <:Y__7LgΜj{mkkdLgjjܹs4fJ j---p8.\ʪR8}j5JLc(`r.\(--W|։ .!z-F^|>L_699p:::\.y0Ht3i:QFD0\__L%u;v_5U*41 HT>P(0 &@ E0>Ą\.B=== q3'YTիYL>LfիWrIP]H$Rkx.@,J-;<Eѕxh0,))O& d28qBWWWkH$ rmm-ut:Q~:9D(&5>o2V* сX,٬T*Zd9NѸQQQa4x(G,aRyy"IvY&nۍu\(nooOOOx~!N+fWVVn7 6nb1HqF{{(\y-}`Fs!L&S4'1bti*rx|&b##woi)oW\I$Lȑ#SSS rx<6x<p8LA&L&}wFIqʕCTZZz嵵T*l^d {a' o߾~X,lb8 BESuhT.R! 7`q]oooKK_ AБ#G>x<.Hn\.w:LF$nDbdfEu}q<8pVNA^n5D nnnbM8]+`uuO}MMMx\i<ω}"P','\',xv0e)8*|U3ZDeVaVϓc*"!%mǐt.gI}bSJɪCf>Ki蜕4> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 296 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 295 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 298 0 obj << /Length 21877 >> stream q 15.000 37.642 577.500 739.358 re W n 0.965 0.965 0.965 rg 26.250 642.422 555.000 134.578 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 642.422 m 581.250 642.422 l 581.250 643.172 l 26.250 643.172 l f q 35.250 653.672 537.000 123.328 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 3: Collapsed response rate data from the final 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 1A \(left panels\) and Experiment 1B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 708.729 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 608.201 Td /F4 12.0 Tf [(Experiment 1B)] TJ ET BT 26.250 588.246 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 568.842 Td /F1 9.8 Tf [(A second cohort of BAC HD \(CHDI-007-\(3\)\(1\)\) female mice were evaluated at an earlier age, both to confirm the phenotype was )] TJ ET BT 26.250 556.937 Td /F1 9.8 Tf [(robust and to potentially extend the finding to an earlier timepoint. These animals were experimentally nave and were housed )] TJ ET BT 26.250 545.032 Td /F1 9.8 Tf [(and maintained as described in Experiment 1A, except that they were maintained at their target weights by daily access to an )] TJ ET BT 26.250 533.127 Td /F1 9.8 Tf [(unlimited quantity of food for a 2 hour period following completion of testing, rather than by daily provision of a limited quantity of )] TJ ET BT 26.250 521.223 Td /F1 9.8 Tf [(food. These animals were 46 weeks old 1 week on the initial day of instrumental training and were 55 weeks old 1 week on )] TJ ET BT 26.250 509.318 Td /F1 9.8 Tf [(the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 489.913 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 470.508 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that these animals received 5 sessions of )] TJ ET BT 26.250 458.604 Td /F1 9.8 Tf [(FR5 training following acquisition of the lever press and only 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 439.199 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 419.794 Td /F5 9.8 Tf [(Pretraining. )] TJ ET BT 78.812 419.794 Td /F1 9.8 Tf [(As in Experiment 1A, all animals in this study readily acquired the lever press response, reaching the reinforcer )] TJ ET BT 26.250 407.889 Td /F1 9.8 Tf [(criterion in the initial two days of testing. In the FR5 training phase, both groups responded faster as training progressed )] TJ ET BT 26.250 395.985 Td /F1 9.8 Tf [(independently of genotype \(Session main effect: F\(4,56\) = 25.7, p < 0.0001\).)] TJ ET BT 26.250 376.580 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 376.580 Td /F1 9.8 Tf [(Overall session data from the PR-alone training phase, presented in the right panel ofFigure 1 above, again clearly )] TJ ET BT 26.250 364.675 Td /F1 9.8 Tf [(indicated that the BAC Tg mice earned fewer reinforcers than WT controls, with no major effects of training\(Genotype main )] TJ ET BT 26.250 352.770 Td /F1 9.8 Tf [(effect: F\(1,14\) = 10.2, )] TJ ET BT 122.453 352.770 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 157.963 352.770 Td /F1 9.8 Tf [(; Figure 1\).)] TJ ET BT 26.250 333.366 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 333.366 Td /F1 9.8 Tf [( The number of reinforcers earned in the 8 sessions of mixed FR5/PR sessions is summarized in the right panels )] TJ ET BT 26.250 321.461 Td /F1 9.8 Tf [(of Figure 2, with the FR5 period presented in the upper panel and the PR period presented in the lower panel. Both groups )] TJ ET BT 26.250 309.556 Td /F1 9.8 Tf [(earned similar number of reinforcers in the FR5 components although they earned fewer reinforcers as training progressed and )] TJ ET BT 26.250 297.651 Td /F1 9.8 Tf [(showed some variability during the last few sessions, with BAC Tg mice earning more reinforcers on day 6 \(Genotype x Session )] TJ ET BT 26.250 285.747 Td /F1 9.8 Tf [(interaction and Session main effect: smaller F\(7,98\) = 2.30, )] TJ ET BT 284.469 285.747 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 327.564 285.747 Td /F1 9.8 Tf [( simple main effects analysis: F\(1,98\) = 5.11, )] TJ ET BT 523.997 285.747 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 26.250 273.842 Td /F1 9.8 Tf [(Figure 2 upper panel\))] TJ ET BT 119.460 273.842 Td /F5 9.8 Tf [(. )] TJ ET BT 124.881 273.842 Td /F1 9.8 Tf [(Similarly, in the PR component of this experiment, both groups earned slightly less reinforcers as )] TJ ET BT 26.250 261.937 Td /F1 9.8 Tf [(sessions progressed but this effect was independent of genotype \(Session main effect: F\(7,98\) = 3.59)] TJ ET BT 464.415 261.937 Td /F5 9.8 Tf [(, p < 0.01; )] TJ ET BT 510.767 261.937 Td /F1 9.8 Tf [(Figure 2, lower )] TJ ET BT 26.250 250.032 Td /F1 9.8 Tf [(right panel\).)] TJ ET BT 26.250 230.628 Td /F1 9.8 Tf [(Finally, analysis of the response rate as a function of the number of reinforcers earned was carried out. As in the previous )] TJ ET BT 26.250 218.723 Td /F1 9.8 Tf [(experiment, these data were analyzed only for the period during the sessions where the majority of animals contributed data, )] TJ ET BT 26.250 206.818 Td /F1 9.8 Tf [(namely the first 30 reinforcers in the FR5 phase and 14 reinforcers in the PR phase \(up to the 180 ratio\). These data show that )] TJ ET BT 26.250 194.913 Td /F1 9.8 Tf [(the response rate in the FR5 component declined with successive reinforcers independently of genotype \(Reinforcer Bin main )] TJ ET BT 26.250 183.009 Td /F1 9.8 Tf [(effect: F\(39,546\) = 4.96, )] TJ ET BT 133.295 183.009 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 185.068 183.009 Td /F1 9.8 Tf [(Figure 3, upper right panel\). Analysis of the PR period showed that responding increased )] TJ ET BT 26.250 171.104 Td /F1 9.8 Tf [(with the ratio requirement and suggested a drop in the BAC Tg mice with higher ratios, although the genotype difference missed )] TJ ET BT 26.250 159.199 Td /F1 9.8 Tf [(significance \(Genotype x Response Requirement interaction: F\(12,167\) = 1.49, p < 0.14; Response Requirement main effect: )] TJ ET BT 26.250 147.294 Td /F1 9.8 Tf [(F\(12,167\) = 13.8, p < 0.0001\).)] TJ ET BT 26.250 110.692 Td /F4 12.0 Tf [(Experiment 2A)] TJ ET BT 26.250 90.738 Td /F1 9.8 Tf [(Testing was also carried out in the z_Q175 KI mouse, a novel knock-in model derived at Psychogenics from the CAG140 KI )] TJ ET BT 26.250 78.833 Td /F1 9.8 Tf [(mouse \(see )] TJ ET 0.267 0.267 0.267 rg BT 79.894 80.340 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 89.532 78.833 Td /F1 9.8 Tf [(\), now carrying between 180 and 195 CAG repeats \(see )] TJ ET 0.267 0.267 0.267 rg BT 333.399 80.340 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 343.036 78.833 Td /F1 9.8 Tf [( for detailed description of this novel line\), to study )] TJ ET BT 26.250 66.928 Td /F1 9.8 Tf [(incentive motivation in a second HD model.)] TJ ET Q q 15.000 37.642 577.500 739.358 re W n 0.965 0.965 0.965 rg 26.250 642.422 555.000 134.578 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 642.422 m 581.250 642.422 l 581.250 643.172 l 26.250 643.172 l f q 35.250 653.672 537.000 123.328 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 3: Collapsed response rate data from the final 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 1A \(left panels\) and Experiment 1B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 708.729 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 608.201 Td /F4 12.0 Tf [(Experiment 1B)] TJ ET BT 26.250 588.246 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 568.842 Td /F1 9.8 Tf [(A second cohort of BAC HD \(CHDI-007-\(3\)\(1\)\) female mice were evaluated at an earlier age, both to confirm the phenotype was )] TJ ET BT 26.250 556.937 Td /F1 9.8 Tf [(robust and to potentially extend the finding to an earlier timepoint. These animals were experimentally nave and were housed )] TJ ET BT 26.250 545.032 Td /F1 9.8 Tf [(and maintained as described in Experiment 1A, except that they were maintained at their target weights by daily access to an )] TJ ET BT 26.250 533.127 Td /F1 9.8 Tf [(unlimited quantity of food for a 2 hour period following completion of testing, rather than by daily provision of a limited quantity of )] TJ ET BT 26.250 521.223 Td /F1 9.8 Tf [(food. These animals were 46 weeks old 1 week on the initial day of instrumental training and were 55 weeks old 1 week on )] TJ ET BT 26.250 509.318 Td /F1 9.8 Tf [(the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 489.913 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 470.508 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that these animals received 5 sessions of )] TJ ET BT 26.250 458.604 Td /F1 9.8 Tf [(FR5 training following acquisition of the lever press and only 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 439.199 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 419.794 Td /F5 9.8 Tf [(Pretraining. )] TJ ET BT 78.812 419.794 Td /F1 9.8 Tf [(As in Experiment 1A, all animals in this study readily acquired the lever press response, reaching the reinforcer )] TJ ET BT 26.250 407.889 Td /F1 9.8 Tf [(criterion in the initial two days of testing. In the FR5 training phase, both groups responded faster as training progressed )] TJ ET BT 26.250 395.985 Td /F1 9.8 Tf [(independently of genotype \(Session main effect: F\(4,56\) = 25.7, p < 0.0001\).)] TJ ET BT 26.250 376.580 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 376.580 Td /F1 9.8 Tf [(Overall session data from the PR-alone training phase, presented in the right panel ofFigure 1 above, again clearly )] TJ ET BT 26.250 364.675 Td /F1 9.8 Tf [(indicated that the BAC Tg mice earned fewer reinforcers than WT controls, with no major effects of training\(Genotype main )] TJ ET BT 26.250 352.770 Td /F1 9.8 Tf [(effect: F\(1,14\) = 10.2, )] TJ ET BT 122.453 352.770 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 157.963 352.770 Td /F1 9.8 Tf [(; Figure 1\).)] TJ ET BT 26.250 333.366 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 333.366 Td /F1 9.8 Tf [( The number of reinforcers earned in the 8 sessions of mixed FR5/PR sessions is summarized in the right panels )] TJ ET BT 26.250 321.461 Td /F1 9.8 Tf [(of Figure 2, with the FR5 period presented in the upper panel and the PR period presented in the lower panel. Both groups )] TJ ET BT 26.250 309.556 Td /F1 9.8 Tf [(earned similar number of reinforcers in the FR5 components although they earned fewer reinforcers as training progressed and )] TJ ET BT 26.250 297.651 Td /F1 9.8 Tf [(showed some variability during the last few sessions, with BAC Tg mice earning more reinforcers on day 6 \(Genotype x Session )] TJ ET BT 26.250 285.747 Td /F1 9.8 Tf [(interaction and Session main effect: smaller F\(7,98\) = 2.30, )] TJ ET BT 284.469 285.747 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 327.564 285.747 Td /F1 9.8 Tf [( simple main effects analysis: F\(1,98\) = 5.11, )] TJ ET BT 523.997 285.747 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 26.250 273.842 Td /F1 9.8 Tf [(Figure 2 upper panel\))] TJ ET BT 119.460 273.842 Td /F5 9.8 Tf [(. )] TJ ET BT 124.881 273.842 Td /F1 9.8 Tf [(Similarly, in the PR component of this experiment, both groups earned slightly less reinforcers as )] TJ ET BT 26.250 261.937 Td /F1 9.8 Tf [(sessions progressed but this effect was independent of genotype \(Session main effect: F\(7,98\) = 3.59)] TJ ET BT 464.415 261.937 Td /F5 9.8 Tf [(, p < 0.01; )] TJ ET BT 510.767 261.937 Td /F1 9.8 Tf [(Figure 2, lower )] TJ ET BT 26.250 250.032 Td /F1 9.8 Tf [(right panel\).)] TJ ET BT 26.250 230.628 Td /F1 9.8 Tf [(Finally, analysis of the response rate as a function of the number of reinforcers earned was carried out. As in the previous )] TJ ET BT 26.250 218.723 Td /F1 9.8 Tf [(experiment, these data were analyzed only for the period during the sessions where the majority of animals contributed data, )] TJ ET BT 26.250 206.818 Td /F1 9.8 Tf [(namely the first 30 reinforcers in the FR5 phase and 14 reinforcers in the PR phase \(up to the 180 ratio\). These data show that )] TJ ET BT 26.250 194.913 Td /F1 9.8 Tf [(the response rate in the FR5 component declined with successive reinforcers independently of genotype \(Reinforcer Bin main )] TJ ET BT 26.250 183.009 Td /F1 9.8 Tf [(effect: F\(39,546\) = 4.96, )] TJ ET BT 133.295 183.009 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 185.068 183.009 Td /F1 9.8 Tf [(Figure 3, upper right panel\). Analysis of the PR period showed that responding increased )] TJ ET BT 26.250 171.104 Td /F1 9.8 Tf [(with the ratio requirement and suggested a drop in the BAC Tg mice with higher ratios, although the genotype difference missed )] TJ ET BT 26.250 159.199 Td /F1 9.8 Tf [(significance \(Genotype x Response Requirement interaction: F\(12,167\) = 1.49, p < 0.14; Response Requirement main effect: )] TJ ET BT 26.250 147.294 Td /F1 9.8 Tf [(F\(12,167\) = 13.8, p < 0.0001\).)] TJ ET BT 26.250 110.692 Td /F4 12.0 Tf [(Experiment 2A)] TJ ET BT 26.250 90.738 Td /F1 9.8 Tf [(Testing was also carried out in the z_Q175 KI mouse, a novel knock-in model derived at Psychogenics from the CAG140 KI )] TJ ET BT 26.250 78.833 Td /F1 9.8 Tf [(mouse \(see )] TJ ET 0.267 0.267 0.267 rg BT 79.894 80.340 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 89.532 78.833 Td /F1 9.8 Tf [(\), now carrying between 180 and 195 CAG repeats \(see )] TJ ET 0.267 0.267 0.267 rg BT 333.399 80.340 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 343.036 78.833 Td /F1 9.8 Tf [( for detailed description of this novel line\), to study )] TJ ET BT 26.250 66.928 Td /F1 9.8 Tf [(incentive motivation in a second HD model.)] TJ ET Q q 15.000 37.642 577.500 739.358 re W n 0.965 0.965 0.965 rg 26.250 642.422 555.000 134.578 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 642.422 m 581.250 642.422 l 581.250 643.172 l 26.250 643.172 l f q 35.250 653.672 537.000 123.328 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 3: Collapsed response rate data from the final 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 1A \(left panels\) and Experiment 1B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 708.729 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 608.201 Td /F4 12.0 Tf [(Experiment 1B)] TJ ET BT 26.250 588.246 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 568.842 Td /F1 9.8 Tf [(A second cohort of BAC HD \(CHDI-007-\(3\)\(1\)\) female mice were evaluated at an earlier age, both to confirm the phenotype was )] TJ ET BT 26.250 556.937 Td /F1 9.8 Tf [(robust and to potentially extend the finding to an earlier timepoint. These animals were experimentally nave and were housed )] TJ ET BT 26.250 545.032 Td /F1 9.8 Tf [(and maintained as described in Experiment 1A, except that they were maintained at their target weights by daily access to an )] TJ ET BT 26.250 533.127 Td /F1 9.8 Tf [(unlimited quantity of food for a 2 hour period following completion of testing, rather than by daily provision of a limited quantity of )] TJ ET BT 26.250 521.223 Td /F1 9.8 Tf [(food. These animals were 46 weeks old 1 week on the initial day of instrumental training and were 55 weeks old 1 week on )] TJ ET BT 26.250 509.318 Td /F1 9.8 Tf [(the initial day of mixed FR5/PR training.)] TJ ET BT 26.250 489.913 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 470.508 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that these animals received 5 sessions of )] TJ ET BT 26.250 458.604 Td /F1 9.8 Tf [(FR5 training following acquisition of the lever press and only 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 439.199 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 419.794 Td /F5 9.8 Tf [(Pretraining. )] TJ ET BT 78.812 419.794 Td /F1 9.8 Tf [(As in Experiment 1A, all animals in this study readily acquired the lever press response, reaching the reinforcer )] TJ ET BT 26.250 407.889 Td /F1 9.8 Tf [(criterion in the initial two days of testing. In the FR5 training phase, both groups responded faster as training progressed )] TJ ET BT 26.250 395.985 Td /F1 9.8 Tf [(independently of genotype \(Session main effect: F\(4,56\) = 25.7, p < 0.0001\).)] TJ ET BT 26.250 376.580 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 376.580 Td /F1 9.8 Tf [(Overall session data from the PR-alone training phase, presented in the right panel ofFigure 1 above, again clearly )] TJ ET BT 26.250 364.675 Td /F1 9.8 Tf [(indicated that the BAC Tg mice earned fewer reinforcers than WT controls, with no major effects of training\(Genotype main )] TJ ET BT 26.250 352.770 Td /F1 9.8 Tf [(effect: F\(1,14\) = 10.2, )] TJ ET BT 122.453 352.770 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 157.963 352.770 Td /F1 9.8 Tf [(; Figure 1\).)] TJ ET BT 26.250 333.366 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 333.366 Td /F1 9.8 Tf [( The number of reinforcers earned in the 8 sessions of mixed FR5/PR sessions is summarized in the right panels )] TJ ET BT 26.250 321.461 Td /F1 9.8 Tf [(of Figure 2, with the FR5 period presented in the upper panel and the PR period presented in the lower panel. Both groups )] TJ ET BT 26.250 309.556 Td /F1 9.8 Tf [(earned similar number of reinforcers in the FR5 components although they earned fewer reinforcers as training progressed and )] TJ ET BT 26.250 297.651 Td /F1 9.8 Tf [(showed some variability during the last few sessions, with BAC Tg mice earning more reinforcers on day 6 \(Genotype x Session )] TJ ET BT 26.250 285.747 Td /F1 9.8 Tf [(interaction and Session main effect: smaller F\(7,98\) = 2.30, )] TJ ET BT 284.469 285.747 Td /F5 9.8 Tf [(ps < 0.05;)] TJ ET BT 327.564 285.747 Td /F1 9.8 Tf [( simple main effects analysis: F\(1,98\) = 5.11, )] TJ ET BT 523.997 285.747 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 26.250 273.842 Td /F1 9.8 Tf [(Figure 2 upper panel\))] TJ ET BT 119.460 273.842 Td /F5 9.8 Tf [(. )] TJ ET BT 124.881 273.842 Td /F1 9.8 Tf [(Similarly, in the PR component of this experiment, both groups earned slightly less reinforcers as )] TJ ET BT 26.250 261.937 Td /F1 9.8 Tf [(sessions progressed but this effect was independent of genotype \(Session main effect: F\(7,98\) = 3.59)] TJ ET BT 464.415 261.937 Td /F5 9.8 Tf [(, p < 0.01; )] TJ ET BT 510.767 261.937 Td /F1 9.8 Tf [(Figure 2, lower )] TJ ET BT 26.250 250.032 Td /F1 9.8 Tf [(right panel\).)] TJ ET BT 26.250 230.628 Td /F1 9.8 Tf [(Finally, analysis of the response rate as a function of the number of reinforcers earned was carried out. As in the previous )] TJ ET BT 26.250 218.723 Td /F1 9.8 Tf [(experiment, these data were analyzed only for the period during the sessions where the majority of animals contributed data, )] TJ ET BT 26.250 206.818 Td /F1 9.8 Tf [(namely the first 30 reinforcers in the FR5 phase and 14 reinforcers in the PR phase \(up to the 180 ratio\). These data show that )] TJ ET BT 26.250 194.913 Td /F1 9.8 Tf [(the response rate in the FR5 component declined with successive reinforcers independently of genotype \(Reinforcer Bin main )] TJ ET BT 26.250 183.009 Td /F1 9.8 Tf [(effect: F\(39,546\) = 4.96, )] TJ ET BT 133.295 183.009 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 185.068 183.009 Td /F1 9.8 Tf [(Figure 3, upper right panel\). Analysis of the PR period showed that responding increased )] TJ ET BT 26.250 171.104 Td /F1 9.8 Tf [(with the ratio requirement and suggested a drop in the BAC Tg mice with higher ratios, although the genotype difference missed )] TJ ET BT 26.250 159.199 Td /F1 9.8 Tf [(significance \(Genotype x Response Requirement interaction: F\(12,167\) = 1.49, p < 0.14; Response Requirement main effect: )] TJ ET BT 26.250 147.294 Td /F1 9.8 Tf [(F\(12,167\) = 13.8, p < 0.0001\).)] TJ ET BT 26.250 110.692 Td /F4 12.0 Tf [(Experiment 2A)] TJ ET BT 26.250 90.738 Td /F1 9.8 Tf [(Testing was also carried out in the z_Q175 KI mouse, a novel knock-in model derived at Psychogenics from the CAG140 KI )] TJ ET BT 26.250 78.833 Td /F1 9.8 Tf [(mouse \(see )] TJ ET 0.267 0.267 0.267 rg BT 79.894 80.340 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 89.532 78.833 Td /F1 9.8 Tf [(\), now carrying between 180 and 195 CAG repeats \(see )] TJ ET 0.267 0.267 0.267 rg BT 333.399 80.340 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 343.036 78.833 Td /F1 9.8 Tf [( for detailed description of this novel line\), to study )] TJ ET BT 26.250 66.928 Td /F1 9.8 Tf [(incentive motivation in a second HD model.)] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(5)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 299 0 obj << /Type /Annot /Subtype /Link /A 300 0 R /Border [0 0 0] /H /I /Rect [ 79.8945 79.5384 89.5318 88.3567 ] >> endobj 300 0 obj << /Type /Action >> endobj 301 0 obj << /Type /Annot /Subtype /Link /A 302 0 R /Border [0 0 0] /H /I /Rect [ 333.3988 79.5384 343.0362 88.3567 ] >> endobj 302 0 obj << /Type /Action >> endobj 303 0 obj << /Type /Annot /Subtype /Link /A 304 0 R /Border [0 0 0] /H /I /Rect [ 79.8945 79.5384 89.5318 88.3567 ] >> endobj 304 0 obj << /Type /Action >> endobj 305 0 obj << /Type /Annot /Subtype /Link /A 306 0 R /Border [0 0 0] /H /I /Rect [ 333.3988 79.5384 343.0362 88.3567 ] >> endobj 306 0 obj << /Type /Action >> endobj 307 0 obj << /Type /Annot /Subtype /Link /A 308 0 R /Border [0 0 0] /H /I /Rect [ 79.8945 79.5384 89.5318 88.3567 ] >> endobj 308 0 obj << /Type /Action >> endobj 309 0 obj << /Type /Annot /Subtype /Link /A 310 0 R /Border [0 0 0] /H /I /Rect [ 333.3988 79.5384 343.0362 88.3567 ] >> endobj 310 0 obj << /Type /Action >> endobj 311 0 obj << /Type /Page /Parent 3 0 R /Annots [ 313 0 R 317 0 R 321 0 R 325 0 R 329 0 R 333 0 R ] /Contents 312 0 R >> endobj 312 0 obj << /Length 17598 >> stream 0.271 0.267 0.267 rg q 15.000 22.453 577.500 754.547 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(WT, homozygous and heterozygous \(het\) knock-in \(KI\) mice were bred in our facility by crossing pairs of z_Q175 KI \(CHDI-015-)] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(1\) het mice, on a congenic C57Bl/6J background. A small cohort of female z_Q175 KI mice was evaluated in this study, with 5 )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(mice tested per genotype. Homozygous mice were also included in the initial cohort but failed to acquire the lever press )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(response in a timely fashion and were accordingly dropped from the study. One WT animal became sick following completion of )] TJ ET BT 26.250 700.452 Td /F1 9.8 Tf [(instrumental pretraining and was subsequently dropped from the experiment.)] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(The mice were maintained throughout in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(water. Prior to this testing, these animals underwent a comprehensive behavioral test battery but were nave to operant boxes )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(and had not previously been food restricted. These mice were single housed in opti-MICE cages prior to the start of this testing, )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(with all animals reduced to 85% of their )] TJ ET BT 198.571 645.333 Td /F5 9.8 Tf [(ad libitum)] TJ ET BT 240.292 645.333 Td /F1 9.8 Tf [( body weight by daily feeding of limited quantities of food \(BIO-SERV 500mg )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(pellets\) throughout the test period.)] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(All mice were 74 weeks old 1 week on the initial day of instrumental training and 77 1-week old on the initial day of mixed )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(FR5/PR training. The z_Q175 het mice in this experiment had a mean CAG repeat length of 193 repeats, ranging from 189 to )] TJ ET BT 26.250 590.214 Td /F1 9.8 Tf [(196 repeats.)] TJ ET BT 26.250 570.810 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 551.405 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A. This experiment was conducted over a shorter )] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(timeframe than were Experiments 1A and 1B, with the animals receiving 4 sessions of FR5 training following acquisition of the )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(lever press, 6 sessions of PR-alone training and finishing with 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 508.191 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 488.786 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 488.786 Td /F1 9.8 Tf [( WT and het mice reached the reinforcer criterion after between 2 and 6 days with no genotype differences. )] TJ ET BT 26.250 476.881 Td /F1 9.8 Tf [(Evaluation of the response rates in the four FR5 training sessions \(data not shown\) showed that both groups responded faster )] TJ ET BT 26.250 464.976 Td /F1 9.8 Tf [(over the course of training independently of genotype \(Session main effect: F\(3,21\) = 16.5, )] TJ ET BT 419.438 464.976 Td /F5 9.8 Tf [(p < 0.0001\).)] TJ ET BT 26.250 445.572 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 445.572 Td /F1 9.8 Tf [(Day-by-day data from the 6 PR-alone training days showed that WT mice earned more reinforcers as training )] TJ ET BT 26.250 433.667 Td /F1 9.8 Tf [(progressed, whereas the z_Q175 mice were quite stable. Both groups performed similarly at the end of training \(Session x )] TJ ET BT 26.250 421.762 Td /F1 9.8 Tf [(Genotype interaction: F\(5,35\) = 2.98, )] TJ ET BT 188.568 421.762 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 224.078 421.762 Td /F1 9.8 Tf [(, Session effect in WT: F\(5,35\) = 6.32, )] TJ ET BT 391.261 421.762 Td /F5 9.8 Tf [(p < 0.001, )] TJ ET BT 437.612 421.762 Td /F1 9.8 Tf [(Figure 4, left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 270.322 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 411.881 m 581.250 411.881 l 581.250 411.131 l 26.250 411.131 l f 26.250 270.322 m 581.250 270.322 l 581.250 271.072 l 26.250 271.072 l f q 225.000 0 0 84.750 35.250 317.381 cm /I28 Do Q q 35.250 281.572 537.000 29.809 re W n 0.271 0.267 0.267 rg BT 35.250 301.857 Td /F4 9.8 Tf [(Fig. 4: Number of reinforcers earned during progressive ratio training across sessions in Experiments 2A \(left )] TJ ET BT 35.250 289.953 Td /F4 9.8 Tf [(panel\) and 2B \(right panel\))] TJ ET Q BT 26.250 253.298 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 253.298 Td /F1 9.8 Tf [( During the PR component both groups showed variable performance across days but without a consistent effect )] TJ ET BT 26.250 241.393 Td /F1 9.8 Tf [(of either genotype or training session \(Session main effect: F\(7,49\) = 2.75, )] TJ ET BT 349.511 241.393 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 390.442 241.393 Td /F1 9.8 Tf [(Figure 5, upper left panel\). Analysis of the )] TJ ET BT 26.250 229.488 Td /F1 9.8 Tf [(PR data revealed that both groups earned less reinforcers as training progressed independently of genotype \(Session main )] TJ ET BT 26.250 217.584 Td /F1 9.8 Tf [(effect: F\(7,49\) = 7.70, )] TJ ET BT 122.453 217.584 Td /F5 9.8 Tf [(p < 0.001)] TJ ET BT 163.384 217.584 Td /F1 9.8 Tf [(; Figure 5, lower left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 22.453 555.000 185.250 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 207.703 m 581.250 207.703 l 581.250 206.953 l 26.250 206.953 l f q 225.000 0 0 169.500 35.250 28.453 cm /I30 Do Q q 35.250 22.453 537.000 0.000 re W n Q Q q 15.000 22.453 577.500 754.547 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(WT, homozygous and heterozygous \(het\) knock-in \(KI\) mice were bred in our facility by crossing pairs of z_Q175 KI \(CHDI-015-)] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(1\) het mice, on a congenic C57Bl/6J background. A small cohort of female z_Q175 KI mice was evaluated in this study, with 5 )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(mice tested per genotype. Homozygous mice were also included in the initial cohort but failed to acquire the lever press )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(response in a timely fashion and were accordingly dropped from the study. One WT animal became sick following completion of )] TJ ET BT 26.250 700.452 Td /F1 9.8 Tf [(instrumental pretraining and was subsequently dropped from the experiment.)] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(The mice were maintained throughout in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(water. Prior to this testing, these animals underwent a comprehensive behavioral test battery but were nave to operant boxes )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(and had not previously been food restricted. These mice were single housed in opti-MICE cages prior to the start of this testing, )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(with all animals reduced to 85% of their )] TJ ET BT 198.571 645.333 Td /F5 9.8 Tf [(ad libitum)] TJ ET BT 240.292 645.333 Td /F1 9.8 Tf [( body weight by daily feeding of limited quantities of food \(BIO-SERV 500mg )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(pellets\) throughout the test period.)] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(All mice were 74 weeks old 1 week on the initial day of instrumental training and 77 1-week old on the initial day of mixed )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(FR5/PR training. The z_Q175 het mice in this experiment had a mean CAG repeat length of 193 repeats, ranging from 189 to )] TJ ET BT 26.250 590.214 Td /F1 9.8 Tf [(196 repeats.)] TJ ET BT 26.250 570.810 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 551.405 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A. This experiment was conducted over a shorter )] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(timeframe than were Experiments 1A and 1B, with the animals receiving 4 sessions of FR5 training following acquisition of the )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(lever press, 6 sessions of PR-alone training and finishing with 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 508.191 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 488.786 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 488.786 Td /F1 9.8 Tf [( WT and het mice reached the reinforcer criterion after between 2 and 6 days with no genotype differences. )] TJ ET BT 26.250 476.881 Td /F1 9.8 Tf [(Evaluation of the response rates in the four FR5 training sessions \(data not shown\) showed that both groups responded faster )] TJ ET BT 26.250 464.976 Td /F1 9.8 Tf [(over the course of training independently of genotype \(Session main effect: F\(3,21\) = 16.5, )] TJ ET BT 419.438 464.976 Td /F5 9.8 Tf [(p < 0.0001\).)] TJ ET BT 26.250 445.572 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 445.572 Td /F1 9.8 Tf [(Day-by-day data from the 6 PR-alone training days showed that WT mice earned more reinforcers as training )] TJ ET BT 26.250 433.667 Td /F1 9.8 Tf [(progressed, whereas the z_Q175 mice were quite stable. Both groups performed similarly at the end of training \(Session x )] TJ ET BT 26.250 421.762 Td /F1 9.8 Tf [(Genotype interaction: F\(5,35\) = 2.98, )] TJ ET BT 188.568 421.762 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 224.078 421.762 Td /F1 9.8 Tf [(, Session effect in WT: F\(5,35\) = 6.32, )] TJ ET BT 391.261 421.762 Td /F5 9.8 Tf [(p < 0.001, )] TJ ET BT 437.612 421.762 Td /F1 9.8 Tf [(Figure 4, left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 270.322 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 411.881 m 581.250 411.881 l 581.250 411.131 l 26.250 411.131 l f 26.250 270.322 m 581.250 270.322 l 581.250 271.072 l 26.250 271.072 l f q 225.000 0 0 84.750 35.250 317.381 cm /I32 Do Q q 35.250 281.572 537.000 29.809 re W n 0.271 0.267 0.267 rg BT 35.250 301.857 Td /F4 9.8 Tf [(Fig. 4: Number of reinforcers earned during progressive ratio training across sessions in Experiments 2A \(left )] TJ ET BT 35.250 289.953 Td /F4 9.8 Tf [(panel\) and 2B \(right panel\))] TJ ET Q BT 26.250 253.298 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 253.298 Td /F1 9.8 Tf [( During the PR component both groups showed variable performance across days but without a consistent effect )] TJ ET BT 26.250 241.393 Td /F1 9.8 Tf [(of either genotype or training session \(Session main effect: F\(7,49\) = 2.75, )] TJ ET BT 349.511 241.393 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 390.442 241.393 Td /F1 9.8 Tf [(Figure 5, upper left panel\). Analysis of the )] TJ ET BT 26.250 229.488 Td /F1 9.8 Tf [(PR data revealed that both groups earned less reinforcers as training progressed independently of genotype \(Session main )] TJ ET BT 26.250 217.584 Td /F1 9.8 Tf [(effect: F\(7,49\) = 7.70, )] TJ ET BT 122.453 217.584 Td /F5 9.8 Tf [(p < 0.001)] TJ ET BT 163.384 217.584 Td /F1 9.8 Tf [(; Figure 5, lower left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 22.453 555.000 185.250 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 207.703 m 581.250 207.703 l 581.250 206.953 l 26.250 206.953 l f q 225.000 0 0 169.500 35.250 28.453 cm /I34 Do Q q 35.250 22.453 537.000 0.000 re W n Q Q q 15.000 22.453 577.500 754.547 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 748.071 Td /F1 9.8 Tf [(WT, homozygous and heterozygous \(het\) knock-in \(KI\) mice were bred in our facility by crossing pairs of z_Q175 KI \(CHDI-015-)] TJ ET BT 26.250 736.167 Td /F1 9.8 Tf [(1\) het mice, on a congenic C57Bl/6J background. A small cohort of female z_Q175 KI mice was evaluated in this study, with 5 )] TJ ET BT 26.250 724.262 Td /F1 9.8 Tf [(mice tested per genotype. Homozygous mice were also included in the initial cohort but failed to acquire the lever press )] TJ ET BT 26.250 712.357 Td /F1 9.8 Tf [(response in a timely fashion and were accordingly dropped from the study. One WT animal became sick following completion of )] TJ ET BT 26.250 700.452 Td /F1 9.8 Tf [(instrumental pretraining and was subsequently dropped from the experiment.)] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(The mice were maintained throughout in opti-MICE cages \(Animal Care Systems, CO\) on a 12:12 light cycle with free access to )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(water. Prior to this testing, these animals underwent a comprehensive behavioral test battery but were nave to operant boxes )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(and had not previously been food restricted. These mice were single housed in opti-MICE cages prior to the start of this testing, )] TJ ET BT 26.250 645.333 Td /F1 9.8 Tf [(with all animals reduced to 85% of their )] TJ ET BT 198.571 645.333 Td /F5 9.8 Tf [(ad libitum)] TJ ET BT 240.292 645.333 Td /F1 9.8 Tf [( body weight by daily feeding of limited quantities of food \(BIO-SERV 500mg )] TJ ET BT 26.250 633.429 Td /F1 9.8 Tf [(pellets\) throughout the test period.)] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(All mice were 74 weeks old 1 week on the initial day of instrumental training and 77 1-week old on the initial day of mixed )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(FR5/PR training. The z_Q175 het mice in this experiment had a mean CAG repeat length of 193 repeats, ranging from 189 to )] TJ ET BT 26.250 590.214 Td /F1 9.8 Tf [(196 repeats.)] TJ ET BT 26.250 570.810 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET BT 26.250 551.405 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A. This experiment was conducted over a shorter )] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(timeframe than were Experiments 1A and 1B, with the animals receiving 4 sessions of FR5 training following acquisition of the )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(lever press, 6 sessions of PR-alone training and finishing with 8 sessions of mixed FR5/PR training at the end of the study.)] TJ ET BT 26.250 508.191 Td /F5 9.8 Tf [(Behavioral results)] TJ ET BT 26.250 488.786 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 488.786 Td /F1 9.8 Tf [( WT and het mice reached the reinforcer criterion after between 2 and 6 days with no genotype differences. )] TJ ET BT 26.250 476.881 Td /F1 9.8 Tf [(Evaluation of the response rates in the four FR5 training sessions \(data not shown\) showed that both groups responded faster )] TJ ET BT 26.250 464.976 Td /F1 9.8 Tf [(over the course of training independently of genotype \(Session main effect: F\(3,21\) = 16.5, )] TJ ET BT 419.438 464.976 Td /F5 9.8 Tf [(p < 0.0001\).)] TJ ET BT 26.250 445.572 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 445.572 Td /F1 9.8 Tf [(Day-by-day data from the 6 PR-alone training days showed that WT mice earned more reinforcers as training )] TJ ET BT 26.250 433.667 Td /F1 9.8 Tf [(progressed, whereas the z_Q175 mice were quite stable. Both groups performed similarly at the end of training \(Session x )] TJ ET BT 26.250 421.762 Td /F1 9.8 Tf [(Genotype interaction: F\(5,35\) = 2.98, )] TJ ET BT 188.568 421.762 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 224.078 421.762 Td /F1 9.8 Tf [(, Session effect in WT: F\(5,35\) = 6.32, )] TJ ET BT 391.261 421.762 Td /F5 9.8 Tf [(p < 0.001, )] TJ ET BT 437.612 421.762 Td /F1 9.8 Tf [(Figure 4, left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 270.322 555.000 141.560 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 411.881 m 581.250 411.881 l 581.250 411.131 l 26.250 411.131 l f 26.250 270.322 m 581.250 270.322 l 581.250 271.072 l 26.250 271.072 l f q 225.000 0 0 84.750 35.250 317.381 cm /I36 Do Q q 35.250 281.572 537.000 29.809 re W n 0.271 0.267 0.267 rg BT 35.250 301.857 Td /F4 9.8 Tf [(Fig. 4: Number of reinforcers earned during progressive ratio training across sessions in Experiments 2A \(left )] TJ ET BT 35.250 289.953 Td /F4 9.8 Tf [(panel\) and 2B \(right panel\))] TJ ET Q BT 26.250 253.298 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 253.298 Td /F1 9.8 Tf [( During the PR component both groups showed variable performance across days but without a consistent effect )] TJ ET BT 26.250 241.393 Td /F1 9.8 Tf [(of either genotype or training session \(Session main effect: F\(7,49\) = 2.75, )] TJ ET BT 349.511 241.393 Td /F5 9.8 Tf [(p < 0.05, )] TJ ET BT 390.442 241.393 Td /F1 9.8 Tf [(Figure 5, upper left panel\). Analysis of the )] TJ ET BT 26.250 229.488 Td /F1 9.8 Tf [(PR data revealed that both groups earned less reinforcers as training progressed independently of genotype \(Session main )] TJ ET BT 26.250 217.584 Td /F1 9.8 Tf [(effect: F\(7,49\) = 7.70, )] TJ ET BT 122.453 217.584 Td /F5 9.8 Tf [(p < 0.001)] TJ ET BT 163.384 217.584 Td /F1 9.8 Tf [(; Figure 5, lower left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 22.453 555.000 185.250 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 207.703 m 581.250 207.703 l 581.250 206.953 l 26.250 206.953 l f q 225.000 0 0 169.500 35.250 28.453 cm /I38 Do Q q 35.250 22.453 537.000 0.000 re W n Q Q q 225.000 0 0 84.750 35.250 317.381 cm /I40 Do Q q 225.000 0 0 169.500 35.250 28.453 cm /I42 Do Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(6)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 313 0 obj << /Type /Annot /Subtype /Link /A 314 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 317.3813 260.2500 402.1313 ] >> endobj 314 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure4.png) >> endobj 315 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 316 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 315 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 20005>> stream xyxE8^3ܙ@$p+9uUuA]D||8˗={ MF7|M b]]c bjjoDQ+?h<<tGuVQQѓO>K/UUUuuu)))ruu=|MMVaZ_,[:?.˽***3f̐H$}}}oDZ WX-,,z}[[[~~O>Q555aAlllĂ_Wkk ޼y8>>_Dx5T4....\xΝaÆ DbpOoootTpv̘1o+pРwy'##ƍOjΝ~7 E.]rʎӧO?|… ΥKjjjƎ;|ބ5kWTT۷/++k̘1vZXX_򗴴欬۷o>|<55+**)))|իΝ|+W_~޽{&L2eJ[[Q^xJP(ZZZ~i^9sʕ+/<}+W^rS0 Rx`޼y,do޼ISdW^`^<>uuu===ATN6 UbX[[wޙ;wnBB#00:cƌ+WÑe˖M6mʕ?3<&HIFF` B3<GJKK9kkkj ѣFϺv-Jy5R)7fTTg}W_(ŋ֬Y#JSRR6mdeeuԩ^{WbŊ _Ν_gff>͓d{ꩧ{=H_SS#---p)66v˖-9993!ݻ.]*7l؀?___hH I`4YdD8UYYP( ;bss]P&*QVwttA%M4g}ϯ833s騶+V7o޼/eRRܹs A #){!Hd 8``e``a+;vSؼyk׊,--?Ç3fŊ~_pd2Yhh(7J$AAA666~&>>~Ϟ=۶mh4s3f q#FHLL<{̙3 <<<,--%[om۶#G/^sN//QF.YdG]b;::N2wߍ0aqUUUP;w5j$󳳳srr8.<<|ʔ)_~U`_P_P&6zh>֭ƢAjv޽}A|駃7nheefD%D^awwwo  iɒ%|mll`mFQ.A:Saoo@ "ARd%ZL޼yرc_}: _j޽{W-)QnOOBJ,E]T=ٙ#FP(ׯ^hj%v+hXc4\QL*"C&4w04Z555.]|[[[QZFb|)b1fcE.TnJ|*@dx2(j7dc5!**ƍ ߇T0 45j#&jj&#(4CU+5UQ)Zc{{{CBBlll ZffΝ;Hrl gTZ_$иĨϻO2C70L5662"(I$$H^߹smT**!勊,,,֮]wޞ!sZZZjjj09DjNHׯ[[[\~}dǎT㤂v{ U vvv]]]sˋ=3`;/^4(?WX!˩7nhiiY`hJe^^^^^^aaaCCիWϟ띝BCCCBBBCC---?Cn`7n;v믿C{K_W_}奥8::FEE655XZZ?~*//복 *J&޻w?$$dܸqǏ=z֭[CBB-Zh:;;*+++*****jkk333]]]9s愇S_b8۴iSee_|N"7 qN"ꫯYn.tb%̘SxCgN`#JbEHO2pd׮]]]]xi4gee*Fႂ &9r:44t֬Y3g TW\ioo={6Z`QwOBuJJa')F-1b2ױq2"bvQȒPdل&''߻w`05*""bԨQ[n9s%Kz7#!!! |[Ռ3/^B@`ttNknnOZ^iӦ*XӦM+,,T*֍p-T*ZɓIbҍ2^ g_/0RJ`-J !PsԺǺÙQTmmm؞5F-3ܹsg%ܹsÇK>&""b…(WWWf̘d8qEGG̙3  YKK˨ȷ~/o=zoڵ>>>򩧞6l`裏,,,^~e2#677_r _8Fbrb{ڡTԛܷ25]ͤ]$Byyccc;ZQ|EkDՖ޼y͛%%%nnn111sݵkSLL̳>+d 3r;vHAE GU]]sN*T*ݱc#ǰR(@)rL#hb&6yrTUDI QY OLHPtI^yJ'U f;WTT455ϟ6mڶmt:ڵk]vO?tٲepDS,1]%ٳ?Z-)bŝ|8oy£Z9f '|uI\ʏy…c/fA0R4** PRRnݺ .xxxU"C٭_^ !oRY`AAA\U{xxYZZJ"d x]í ~?ʲ0Td/3}:ÞefW !?9{}q7npww ڲe˭[ dVT&''9sf…2ĉUUUMMMOO_> zp۷og}rS"00pݺu^oooBB^<>|W5ӧ3 ͓ WFEEM<"hZ++nxK88F1PGH&T Q!H v!<8Uz'Ϟ=HIIR1clhT{nMMMllU>3ĄRbMeóg&''K?O|?񏮮/^8`0{Prss\+H6lؠ +G{{XTk.>'˗c&sd|9<(DRWW /=ztѢE؟a*H4*B= uPrP.!0[og c9`着_GQ^^p}͛I㜜klmmớ: boMOO7oޞ={BBB!#F0!L&O>=))_~뭷$D"ill{=5IjDPev|Ȧ&8ncc8~M2Jq3 11hH PB<#P_.8~|ŵpDT޻w/&&2`ƍ...V<^^^pE &@^IHHz믿饗㸸877W_}Uiii<F=.Ċ %C?'\x͛7 }SSS]]][[[oo/GD;v D M1i'l.nbIRq* 5*ehd2ѣ9 {%I||<8 Gŋo߾ R728ȑ#3gΔJىAAAXY!rss\-)d$P"cd9{)))݃[o_@c$ Q\I@Ʌ CakN YFĤTr>Ǐ)!e&#aܹ؟cJo8nŅ 3fԩSoܸacc3{`FQӟ$ ?B-L3ňʊ w5uT1XdF 5TL9!􊊊_~ޞ̖~#Glٲ?`( fggtMe\CD2mڴɓ''LⓋU*›IϽ{:88p7g771c,[,""^/b#3R,ʄA6ـ+kkիW'ȵNiӦ`hB~MHHbW111 ƁI P(EEE=:(l9RUU닮&LP(_>ydNf͚_]?oR)b_R̋bKm󅅅nnnʘI^rbh܆حguuuEEEgg'+ :rӧ=zrGyAm_ jdr F?}.++xf?J>)vI*y(97|hnG.!7mooOLLtvv>y$#?UYYh"3DOŐtww8q"&&<;"A|y7n_~…W^d[f&}F`2&V6ki:;;߿?,,lٲenq+ح|&ĬXͻ0 `޼y(!:^_^^w^LАP(Gvvvvvv?`q]]]?c||g}@*(b"BNyxR6lPYYyС04IH-]&Cf2%&iݸ>bl9请tr>3$g >aggߌ'DN\ZZzy湹hjugg'RՅ;?IFcKKKVVVccի}__@>===ɾ Nh4mmmmmm---jZVxzz޿3mB_.ɔJeDD>k&+q'Oܺu5kvލEiEM>,[hJH%!qRT&Fwliiqٳy;1e ٳ׮]ۺu2z'++AAAV 'J-,,,,,#$NNN^^^ӧOOOOtuu]f &'//ڵk7o8n3f̘4i\TQ[[[]] ammm4+++ CkkZh4r+::zҥɰ`S8j  5~ ^o4R}}}{{{Q/a ؜ \lvmiiO]… /Ud#XjP&"r&K-d* 0|ddsӃC0B+Ü9sz=@T!D{ u:Mpp0|eܹnnnj'u3`4]v9R]?vym޼59288811oWyYjuv???4BQZZ`mm-˭zzz{{{]]]<==JF222xK.wPIw5۶mr@;YuԔ}ÜFu6؂Rq0jT=quO<Uon?l"&&Vy  ;wnPP7[=//_c"ѡR?$&&Bf̘§Նg7ˈ#ÇuuuG}}}1OOOWWWXDcǎ7xQxXtǏ_VV+SN(@xig ``y1۩Q3BaՉ s Փ: RDՙ`Rv ǥJ1$$$$$dڴi/4Fkaa[o>}r'liiٱc뽼;vɌM5G?4iƍ &L;1f#dDkhhP(NNNs28 Em޼Y*7i8͓Tn$˃"T q bIyW"899}waaaRKfìY6nhggd|lA"1zzz.\?Ο?Zeee%%%0uV&mذ!))7߄30 @(-- I04th4c0zzzryMM |ڕ!iӦ nDF: !*r'2Tel3;!d$J$8|@CCv6 9r^?~~Ietׯ_?rHQQQBB͛###uG34wss[f |©@yyyiiimm%0bĈ?|P޻woܹ7oބ/-///..vvvMKK3RGGGW?]"<lI`)D9D"sss>ccc EGG_VDN>ekk7?><<#D"W1dPTTt_~eԩSa O\\\K#``8suu}!Tl0777::ZT/z {JS*p0))СC|I0rAwfl+3F "֊%GN޽{:$$T__?bĈ|%==}֭&M"NRj rJhhFٳgσR#&L0nܸ#G!ìP( FǏ={_O+QB;s SFi f#)SL@%[Ԓ 9S*---ϟ֭CovJ$ꉙ+B(c^S?$vF0򊌌,,,3 eəÆ .tssOq $...##CP/8R(ɥF1,, nԨQ'N3gΜ8qϟ9s ۯ5z ^&cH/9 :uBv  000TꆍW:ǜ B5#V9m ?%#mll=Pl&Ãz^Frtt/rrr>\RR 6a„;w޺ugٻwoppfy!Z " `z05e1r>R RFL1JꃒP4ucU:.Fh^z(D??Q!!!7n܀&ㇹF'@.?~ܸq<''端ڱcǰavVFMPAK2ߩb1GQ9cIqSBYVy?9R*-=b ~,ũja>69;;1cDGGϜ9_~eO?>-TeH̭lB1B9@ YI%%L&rww.vɃ(5wy!w]Շ "N3"#(gxVTO$V(S* Wtv*Y77SNYZZ*7| ejЌ k@&C;U:RGJ(@^"\t ^xd6P+U(j@Ѩ`mm oƘ6EM,IHFBxvQa„ gMcQeW,J4=H@irPhYJ0Ȗ&$ц w̙3ȑ#cƌBqDҤ#Ft:E &-$Kd;h ҉1>t萫kss3#d)恘$ATFP[D!zFlmm` '3`ܸq$?CC1L$i۠l7{0zxjFcggo(!ܘ`3+i  OӋ9%ҫh)HG@a3P\.߳g)C`!QEl$ЕRj2y)K &_2&ԆKR pEn8H?O֣a z,S+D L,@ؒߤBI&D`擦"`f-$lSR7/TH Z;vMS q ։mǥw{]H^gIBR+Bm9@9S0Hq(C14* Uy1+C[!Θ4fhԈ)&P^7@f`# fJZu< lܯj$\IdCI8%\&,ɧ9-- >09)ƁD,gԶImdfDP0 MIm/_,;;`PE6fE(~ xY``sb~'[,%'I*m)kjju֝;w좚#d2v,T( 4aBʋ1=f)7pfDp9!v}뫪"]L=f` ]$ B"N[+["G8q"|ԢE===T"a(c2 qbz[[!UEqR" $H${{ qF 'P6C13 @lvttX  P'x*aHC%HA߶F*ʑ#GB^xBb9 ~׸eHA3%; ŋc`n& ](. $|&?Q!'d2CЭb6-73@t8@L1oAtvȚ7%"3B̮R`et !hWܶcq!uM95-!y<H*mgmЋ\DPXQiGP|hLJVqE-Nqn@6ֹ4%_BU D5 nFΠҊ1*K0D!)& c )naPHBG @`cP-'Ѱet&3"#*K=nSE`&01/j8R:ϟ]TyndԨ> [C-Uy1"ڢdYr ?nE ۰q,lF&JB&ZhԆ` -(C !8hm\ؒQ#S L%gT8AT :ر OZ`-I(RIlRg(ժYa2*l*3i2V!l&hB9" e@u] * 3V1Q QXR"(Ft yA *uUA$h Qd+S& l>)1 o+!&"@6AC"xkB*.\)v ]i6°fBΒK!#FS j(D5DL<`o<}T Fu# 砨L:Ƚ*1-6()cJAܶ$f߂96Cy0ؠ𝞁/A5ݴǻ GY?NR__ѣGcS"gRῒ U}z`i|e_F8U.=" j| _BĐerxsH)C.Y$55u$11СC  Zjڴi=T}}}QQQWWBVקj~KcwпF qpp())# .uppXx1Cܞ~iNrry87n^v-իWf\rbधWVV //`TVV~7۷og}w[ne8p`ڴi! ۷o=3ǝ9sf˖- BRZV-))6V|(?Ν; 3g8pΝ;TArC|{U555H0 ŗ/_>uOKWT={CUUŋ<($O>!?<88 z}bbbQQѸq$6E<ܨ( !4!&)))ƍc 7|k4mllK{{{x7^&NF KKKGGǴ*hJ...---Æ #q8SZÇ^^^T0iҤ:,Jjhhprr1birPTAAAF"dff7.55IGz(~!_D봴41 Q===ǎ+,,5 ٚSAP޽wa }7 z}{{}%%%z+ CGGh4Bax>z`0PqڒP^O ֶo>Ңjlnnvڑ#GJ%#|R3?@Np]// X[[ۃ_ i?]\\M6zh*D$-;;{!!!٪ݹsg9r$[C*y<9NB?>55]]]NNNZΝ}v{{?Vp¹sܹ#!.ct:_]]M5D_x>>>0 ҥKΝ+))`4322Ο?&oooxꩧ^{5Ta2LJ$L&JϞ=xblz5v}Lhmtqpp8uI؈`;::yZ1رcuuuOFB EC8uRΝI3?սth׿vmN7aggS ޽{233 ĉrQF~?I&Ϛ5 ˟}4t111vvvbmmm%&&6..}ǃzСckk qP>tIHHѣGN:mwppHHH 3wޕH$/_NdD7VQy&.\pvvwoֆjSM]VrwwX!=IIIuuu-))ydB___JJ[qq /ovzX՞9sF*:;;M%%`0)D5Luj%,J_}Uf HR{{{//^x߆aBϟO0ܡ:;;Sd0"""Lot8.88Xь?>** *;u@t4lذ'|R.2aaa3cǎŤ222?Wɔ|N۷s̱ԕ{g0ZXb Cz)\q=<<ȭ Մ~ڴi'N  N<9~0ZD$3gBBBƎ[__?f̘1cyWrFCXtiII %~UVVzzzZYY yrѢEMMM| ,Lz̙3Gt:F6iqqqRzM̙33 !0OIcbbBBB[&Q,V3w܀ W lrM:ttt|wgmgg}ƍ@ln[c D ? ٧[ظHRq"#Fj#QYr0*a|H !Hء-ӤQh7' DLmqG P̋gT:-T#sQ y$C1IBc8ȯ_Pdbd(!&5y&!` uq 0(իEI$"Id 7Hk#9_~C#8,_-R[ѯeojjj<8>xUT*JT2XtttC tn8`0ܽ{6??㸚'Aףvuttdgg?|H]@TƜFu/Q4pclmm7.C䮮.Nq\CCCvvvnn.s~{{{__dCooJ"7oh4';;8z?*q.ꦦ>`twwcGR#w ]#6m[{V6/Էkkk Saa!2l߾}<0a|Kcccee{322jkk;;; &JyA------~+W[ Eee%<%%eĉ)))~~~cǎqFkkkXX\.OOOoiiqss+((?yq㲳}}}o޼P(oݺ9  X60p0!qTZlkk+//U*]AFG5jmVVF)))qpp(..6 A[n;aaa^#X]] veee{xxH$ӧOO:~rrrʂ߿:22~...̌~{NN^ijjh4* ޶>|p,dx {G%e*{?ؿii)g>}zFFƾ}_'NX]]cmmmmm$,,,T*UsssGGG[[T* tˬe2`ٲe!!!RRxÔD"quutR||ɓ'}||Fc``T*겱1 555>>>ӧO/))섍VBlM:PB\!I)DZ/^p0nܸw}W&{2L&T*ӦMjժ;wz@MMȑ#KJJjCxxx___```AAANjggw۲e!T*urrtAAAEEEqqqݩ򊇇Ggggjj9l[[[lmmcǎU:~xx/| DxOnQhA?s}WyYfErwwW*naÆuuu)fX_un^x>wqرc/\骫ju[[N Q(666α< 1 NNN&::g͚vyF[3rtҥKjunn#GYWWn/// L]*CBB|}}%/pvvkXZZbƔw8qm-888--㸪*Zhmll`GQSS~](E.`$$$\|9""{Z s|p08|H8Fov2!j{-D"πzzzb&"@vz`߱V\\lii9uTT$ ᐟU*-^g*&ޤB(>OB3Tg،IPМmH`9Lz \Y[ c5&1cP|AߨXMTMfl1UٻYzB"{" $OK7cJԠ $lMnٟb I%d :.^ endstream endobj 317 0 obj << /Type /Annot /Subtype /Link /A 318 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 28.4528 260.2500 197.9528 ] >> endobj 318 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure5.png) >> endobj 319 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 320 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 319 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 41263>> stream xw\?>z4!"؈%h4[,c4hhQ4*(" (H* swݳzy<<>gv8q "`xȁTP)&&A$ d~&X4&8,)QQuZ ".}TTvuuADbV[[[^^TTT~7^vfmmm-aRdeeeuuuPgϚ` N8!/\p}΍7‡+WdggGDDǎST\z/111&&4..cΝkuuL&njj*--Qmmm*z2憆7n|$|W"h۶mǏbqCCaRYYYQQQgggkk+4^$AW777K$,TaX,.**ڰaL|rXT666fgg+ LVUU^__P(Riuuu[[aXgggii)Drfav`յi& `ɁC| sܼ\T{xxx?sذa<=|P"ttt؜;wӣG^r~xxxpp^[[{-PW_|CCëWvww:uJP:tHKK;rHKK=<<>SJ驣3uT>ogggkk{O?444tĉeeeмׯǏ뭷v5a„ѕuuul f̘T*#""N< ‹/zzzܹ˞}D"x񢏏ObbbZZZ|||eee``Rwɓ+++ry[[˗-,,rrrlmmmmmR-yB%|SqqqKK˄ >}JKK9RK,--kjjpJϟё[WWwT@QQLP(233߿eff{YfY[[>|3!!811,44СC&MڳgϿ|rPPr͛77nSRRf̘i&++7oz{{:u ðð77gd_UT&&&~_f ;w… 555Ϟ=kggWSScbbRVV[Ž_~ӧeee~~~aaa&M"gXɝ,%JR鵵hX[[[[[Rm۶gϞQF988m޼y֬Y׮]`dd~zboo޼lڵkYYYuuuݳܼy3:::g͚E?!!)<<޽{*ߟ pŊ۶m333[vrqqqqq9tʕ[n޽?iF5{l̙3̙󎎎Jcddm۶s疗[B?0455sKKKmmmSS СCAAAo֡C~嗳gLX222 E__ 477_?Nbf\WWf͛wÛ7o̙C <<<֯_f͚5k_v!//e˖}eeecǎz~> jkk͇ F=zڵk?裥K>{ رcɒ%+VzG222n߾qƑ#GB6///'''gg˗=ȨXx-[ꈐ~{֭555?;w͛]]]kkkuuuy<^__L&ѣW~ ""⧟~R*<t8kxCT:i$49mڴ[nwvv%%%Æ +((x7[ZZ Ecc#FyDS^>Oncǎ駟(ux={̙C6#y/aNNN˖-~Ç_0h'ɓG #RH155nhh:::Ӄᳱ͛e˖ӧGGGO>6U*)@{{{{{ಲ8w܈2É,4FFFz+++O0!%%:qds1bDjjj}}=& ύjjj/_P(oNʇ;a„'Oܹ355իJ\ 9| OOO孭0DWWB )3&MtǏG 8 :@@7=%3 g͚аo߾;w={ 2d͚5;w477O<<<:::BqLJ r۷[[[Cfmll͛wݻwwuu]r,11qnnnWWӧO_paٲe<{{{ݝɓYfFDDlڴRtӦM7n,--aO8d}}}ǧMsN ChiiA8C Ԅ!zzz6lrСCq8[[[΄nڴ)))iŇR(>ӦMCze겴D jhhoڴ zaÆEEEccc?##9**jҥЁC ?|pxx+B)""nǎ˖-KNN311ٸqcMMMUUĉ! rJ$--9;;'AAAVVVv  E~~>7-,, baaaTB啒sN>:;;wttXXX@3z'|rС'n߾=00J&YXXPq*Fg괁dJLLܾ};(ʇM)GSY -@v?Í7~'OK.D"Z{GK0x<8?"E S$&-@۶mKII?; y뭷4PP̛7j'L*(h": ͋Aw RZ[[?3naa1n8'y{{;::R %_[/w u*HOO7 hjj0񣶱M>ÇsT?Hğۃe7bJ Z؉_"`I(DRRuꔯi+**zzz<<<˟?T*wQ[[+ &L뫧-"@M;j mc t%pQ0Dd٫e*bb#J $kGd~5B(QhҢh@YYY9U*UVVVLL̽{gϞ} GGGRW_-^(ʊGikkM0aܸq555˖- jR,La@4{/x _ٵpB1E A=,i `%y_5`{{'OƏ/ݻoرА6j(zj :;;322=ztԩ6??_ĉs555522b;==($$}̝;/΅)yozɓ'oF,O2eݺucƌ!NH|>l̙3gq\P(!!!%%E$b' -,,,--yyy_~ezzzJJ <|||&O<$OPzqooo@@-,,cf q7w)225qDcccr,UUUFFFBG*3D0 [t)H$R(>T[455Nk.GG˗/kkka*J,Ꚙ?~ҤI#Fe*3T )A%%%ǎnܸ9zLݻ+KCL~O/Ř3gdggl㸎T*2eӧO{~mff&ඞR"""V^Maq4>>>!!m„ 3gljj:r\.߶m$(Zfff.f92##(x@ ͉ ѿL&{.>>ZZZWWk a }ĉB011deeI$T?WHH9 .8fZtTkh4Vm~DW )..O0!66VSS-XB###333u'O 2$$$ĖF@XXؒ%KYrb&R۪Λ7ʕ+SN533xw?}4///!!y8lذ3g]xqd"mmmOOO9͛7̙3o&L,J\]]KJJF xxxP*!C0d~49SôI a;n j ,x+``CCüy󴴴*++===!/Q!i=FV?Ϛ5ҥK---iiiJrʔ)+V8zNXkk7xC-dH6i$ڶlOKKKJJ4/dz6mqb\.?z@ ҊYh.j-!p@%OQ o[pܻw=##q8:9J.. TA{♩(Ȳb!ç'B(+ oܸ{4r\T*MMM> O$Dhhhxī)#'SM @KKsΑٸ7hqmm%K޽ѣ&MڰaJPB[[{СwXҚAG=㸻{GG\Z R) D 0̃ZLKj]LT@J<,D>ej8əd)vuu}!<!0,444 ŋO8ex;@BQ ?(\^QQC@Bwڵ^^^_uFFƌ3v=u^1xOoGGEGbOhp.Li!miUe)D٩T*Ҧe;v?:JyyyfffxvLSXXh``$ѡT*ՖOs̙3gBcҤI6lV.vh>NG2LWW@KŦQ5zQdd2@V&GSkjj233޽KB</!!^HI8Ϗbرc0ٳgϞ={IOO5k?S @ZP3ׯg6$`9x!L_(@2_ǎ@P CCCQHKKɉ|&:#?~޽>H[[I|upp l߾}ĵ% / b'0 68q"22 #jTD/^=MMMǏ/))E*\Aj(vttJE2J._+w+ׯ_W(,i)%㵶cuWu m(puNG.I$FE"Qii,EZ=@ P*Օ555$333 p?qĹs|}}'LL‰g"˸X58i ̟?;rȹs8hѢ5kXZZҖSN͛7t 4'$$7bĈ x3A6YD P%4LNȁ(ܡ6FP(-4 疖&LXhTGzxxϝ;O* B'''xQEE_=jԨ B}:''5 ERʈKi픨*0͛/_ѹr__ߣGd2ˉ=&qvv5jԌ3LMM'X|ҥ zzznٲeȑJL՛.#F0MMMgϞݰaQFF… 2)UR(.ޒCrTɯe%2 A .QS'NheeC=zo_7n\VVָqp!Q00--ðkΜ9xjȐ!SN]j<(((00ַϟGDDܹSSSs̘1bXSSsϞ=WvuuH$}}}===3ZRR2fDb 777---mmmmmmMMM]]] ===@ܹP(6l؀g񏁁 ֭[|AH~P\80CKNN퍋;+**||| ύ7<8m4b*L! z$SI7FbD+$ʆ d={rV\PP,--$I~~~UU QTB[IhKVVV~R? ~뭷ZRZ[j> D'NZXX|7...p _OO@OOVTܼp¡C»e2\.J CRTQFiii566Һ6/Ȝ|>?88xէN:tмy֮]kmm]RR% )]ѿ_q:7nܨ B?~,cbbˉ@~¿jjj?~ٳ[nW_ݽ{^1ӧ γg.ZwީT߼y|g egg/]tӦM~~~IJQ]]sى'.^8///**޽{2:;;9;;ÐqVk $쩘:WMz$T*mii!&0`ƃ~]kڵp6LT^y.10jժU}}}7nI,002rϙ3G*]6/// !H i:=eToPEEŵk׺)Y pb203Ԧz)FP\Ryd999,lBjkk;ONRPPp֭7jii(8Cb:88Q444)-@J8 UTTX,^lYWWoYCNJ?wQ8?gu'^탂Mprrrљ9s&|%'N ISSs۶m3xѢE/]4{ӧOԅ L6mС ¼lbBPBiQf0v WPB.&bj$Pd%9@S.iaݻw$W;q0X_^z՗/_vwwG bTg/NlJ577(ai--*!gKIIImmmGGRfLCླྀ#-䴀8'6oW5%L0Ph@M((=yyyX)-d}VVʕ+CBB$) @&YYYy r>55F;AOO?#L"7&&ٳ~B b~`ff& M033kll"R(xZC;h2ɧPlf1b,rg٢mk)(ZLMMMܹ# *Ǣ@DyU*[nݰaB=nџ֙䴖_#WG͝;əL9hnn~U\vtt(CCCOO7|#!CKV.W*}aRloo ?٩Vbo=hnnbhhhddqH<ƍϟi G1 􌈈 .422600:::?c~+*TJ% yX===AOO4~>oiӦM=ta@0?~ w XXXXMMM H-}H;|' $$9VQ`jدHHUt;@&_Q;v 2QE\|^@JfEiy(*D7o @>Ci'0a„?XWWhB~ $bb K&2 .|*Ck YPJV3UN!hm۶m̙D%0k"H)TKvJ,dDz|vvvvww'/4fӧ듷q\av?$q11%%%-"bKȥp1Pڵ ^$‘::bĈ\b%^(OOF6+W֭[/{ӺKRՒWLLLmm-i $@v/( X @wwwyyyll,GKLVQT*JVt hZOˉG!^,X ) id!|wL&ɲc}YhI, E]^U4W BJUVV6t~'_)FGuuuRRDM'˙qtۯ +ד.8??޽{D emlkJ0JuƍtɎRL&06xeY`7һ%*55uLzi!WTTܻwc_b!vs]8G(C )--eee2J[N<_ڶmAbv\wׯ7n\HHȆ s%%%UTT RvvG}TQQwߡ]4dRrA;gΜ WJ+&GJJNNϷl幹cƌ8^XXH|CR269MřR4/bu^^Y__@./2yyy7o$wf y…20#DH$'NߴiSddH$Zp޽{![ggguuuMMMuu5\$d]]]C?H@P,el$L!2YZQ) uqq1ϯ9T*)K?NE?HL&EpZL6Ms[xv6H4d MMM2%;;{̘10}HHƭ[X[IOE >%\o-[ eMKK{ȑ# fSSS#GBGrrqmmmܹ3|y-\Ϗ|:ܹsҥK EZ꠵׮]+**ruu/f uGAJDڙ3gΚ5 qX\SSTTT4ydwߕY@ DDRui~u#[EAAimj vZ h9(v;__YfO2ngȢȁMMMEEE:-[<<?gΜ[[)S0 (ȯܧH;1A(؝ZBA>Z,8 lnStƍ?XSS~prr&w>(W_}5zhaXllbccF_(^0,99ٳeeeK."8?04iҙ3g6o|…C\vmǎ#G\`iYRU1vww;v 4zhx3=&b{Jox{{}ebzFPt,(ru :PifffvvvWnkk6l߸qF U*U|||vvD"MJJ\]]/]D>qOOOٳoܸ1f̘m۶gٳg/_~̙SN:ð'O\vm߾}...'O177?gaaaAAAqqqSSעE<==---1 ;{lddܹswܙѣGO2 A4=ϟ?d߃aիWG}СP.U%˅e/R[rl?fߺuTˉaXDDDqq_~aXYYٓ'O222rrr0 wwwmiiصk䢢Ǐ'&&\r…oZR*bG@yy{QfH?ӧy<Ν;E"7o޸qѣ)d2YfffRRRJJX,rرk#?&'''''j˖-&&&111 iٲe{}wnM܉>!L"&O /T*-..|kjj,,,V\+++}}}===>}SZ핕C c)T*qQQQoON[ҥKÆ 2d"?TOO` 7]]]3g+)@jjj(\J577',D)fffׯ_wbMpc!bbbbBCCqӳtqqT` Μ9=4&L(,,g1|U*yDhBB[m4RۭYOx*RvH/ }MII INN"<"A:u*vrR!!!V***'dS600rʕ+by<)7N(444%%78vؐ!C/\c %|@ !ڌg$$$KRڂBvj6{]0 miM"ІsH+M;9@&'''CCÒ'p͛i"jhhQE3x޽d%VŴiӖ.]JAkyx<ޅ  k lll F 9s?{o % qc>gΜ'3XZK0rHB3y䚚hѢ;wP 5ZC- 2Ҧa`R;;b{w (kiid2pZՋ/>vdzw!gΜD}YTTԎ;0 ޺ukHHHcc3g"##|Mb0 3gξ}XnKLJ==DFF ~Kҵw\wJ7666}Ņ6  ',#u& #Glݺ766/lmm׭[/Ku58!88;v2ox=Mp1HaCsiSq$ 666<ҥKִ6N`VH$V(|Xo8nccn:&QU$R-dA3AVkKZSPӪd ,,PMdT*KL $+**hdO6-CqqkWWj9# š8`F8^XZ  +S.sHR'Y\vR`m_+? QV3((YZn?|p͚5\6+h &L{I? -   ,mreIr$PtQbXx W&J8 )H:&I& ˗/X$+.Z/xѥ \O |y )( 9 ǘ+$KKvB  MO?D ᘊIE+KGGEt#\ C Zi{-T;"“,{T`&!dQ  vW&72% |fv,f:p\q 9(e :BK{*B]YYYppp^^ĉqqq555qqqĭ+"cc㐐 s\\+7o7Rsq6 _sssꤤ$BF"4yyy9qY&ʏaXIIIzz:0&0֭[ZjhhHHH b?H+',QQQE"QttZtdQ!;77͎Z QQQQb#˗/7xz~Z{n߾܌5 ZZ^^Ύe`_+S`,u .NV̡p/v 0^R +hzhyǴIKd|%Z!\$P%ZDpP=Z ^HU-\BZUY}0LA ހ 'j@JMMmjj"3P@OKKkiihgZZZOOG'OT*(}}},=JKK#!Hh3W,HD"Ǵ ɣR۷9 sG,J233LRRRR8ڀa؉'Gyjb)Yd p iѣGRСCզhnn B aaavvv,z]SSS̽/^U(\߿_^^8dvNHPRR xb^^n*(//?DB)%%ԴёLKKD)))>>> ϙ(,,Ã|-EEE>x`ᔳPzYmmmRR/B>|RTTg`JJH$qss$oAYȤI={e!===ggÇX6113fLJJʨQԚP(?R9 HuuuQQO:;;755蘘3x<''N.f"?~?OyAC x񢾾;T*RkIeeX,GggH$eg655 7n\GGP(L1[(&$$;"蘙9evjƺ2*… 8WVV.4),, ð^v88ye7ٳ{ii-,, 񂂂6f;w0x6B%D"`㖖f%HK9998h8~]ꊊ233U*UrrZ҇b.(*++ S-mm-ӧeΝ;8766#H޽Rz{,ʀxSSS\\\vvӉFoii͝>}5eee3ݺu+'''88X(_f2b~O/vtthkkKNHH(((ʂ/?RABRF1uTuegggeeԌ=ČxNNΓ'OOX奧?|ҤI RSS+**LB\Çϟ?6mțŒ䒒Yf{Dg4>=5 OIIɥKd2ٮ]ݻ"P^^ٹsj'+**.]Դ{nLݗ_~ ^:SP744\t/k'd斖;vk'3K.~gٴ BD"џ}<&KŔYh &&&::: ͭ ׯ3f nܸ;j(2ׯ1߿-n߾}5OOO8D KsNddgHHHQQ;aÆ͞=ӧ### o!Z=!x@7dzEn߾gllaq">$&&(Jsse˖QHҾjW`eh$a">0 _zr"Ceׯ0H|8$Q;}2-?%l!L*Tˠ0,$p :0NbZT KGC R)C-_PX" t"Gd_Yɢ,%ݖ ZM$ %r Y(Of@2xcccbS|ZZիW555nݺmddnggGR U*f2j={DP(PHFFF~~~[[ 99>))k蘔/*999"^(fff_S>L>ddRǔ#,AJT{{{qq1RSSmll\]WWw----Ǐ{%溺:cccmmmNYx8r@0 .**~L&kooҺ}J*))T $%% BR xXа`f!%"RVG-X'!{)++X7UڵkӧO/..vttD:::uuu===r[XXd7o P$ njΒ<396L9m۶I$@CCCccq͛577o-ZTPPlx|>_PX[[+J>/4EPLuV{{{mTTR@"333-ZT__?mmmO:::nnnyyyÇDb\T*kcc@ hnn0L.WWWx)WZ!h }[#ЁJ200H$WWWWT>yn+ՅrnƇ;iG&!MMMzy === USSڵvvvphAARvuud2b' ‘RmmmɻdR311d ӓPfC[BQ__ Z*67}w\TG]veU:"b`}wԠ-ϓĘ1&1k5vaYX?03wR4<393e` vU |Rl4AY[[r^zW8Rj6b?_HRg#dw03rbM 6^G,G'-8>|e. ^%2 x҃F̥ucU#MB.LvW|Ґx!`q ~$#! Qr3 qqL. >NHqէO9;;;::syrrrkkkR)x[]]'J]\\D"QCCÝ;w=$>xf0<==E"V53"*--ljSSS\h|rZZZCCB|}}% xËa\r<0Ʀqp8|kekkkwekkkϜ9@PvvD"jNm èj!N3xSF٦MD,HP8998q"555//M6r<66655533ѱ^ XK0L@@kXXXjj*H߻wo.]ߏ?~\:99gQ>Gx+z~ٲebx֭O}(J#G@욤8Т%gv 7oޜ]VVzk׮999%''oe]8D۷hH$oq)RQYYy]ew'''++KL&W.畕t] Ilŋfsuu^^21cϹs|M{0`@`` 0f/\dӧ2gLL̕+WΜ9#>%KGGG'$$k… g^tI./_|%%%˖-x񢽽W_}e0>S???\խ[˗:twٷo_5==]Vڮ\r۶m'O.++{6ls,˾۶֭AI̜:u}Fh=zT[[jժÇϚ5k^^^ bݺu3gҥK 7lؐeEpW_}~ʕ]tի׀jjjgϞB [KRG*;/00pذaŋzw;v찶~kN7vXy3&!!aǏԩSCCӧO?Cooo`0L4ҥK L0͛ G 6lXhh(˲~!0aaaÆ +))ٹsBG 4rHǬӳgbbb.\q7N0رc C_jJJKKx{I&}aaa3f_8dȐ8p@.k;rHΝ;fmm=|q}:.//رc 9r$::Z$yyy]t 0l0tݪ 2SHuk4'd+>|ӽmڴ9rdquutz~6mXf (۷ɓ'E"QLL̠A駟2 cggWQQqҥq%'';;;K;w,;}t^4iƍ={?1 h _,\pǎJDDį{k?좢>30t_W^=zubb"$l6/_|wᶍZbѣ8O?͜9S2 SYY}A?Ν;/\`0 fΜG1 DfĊW>M:Օ/.\6lÇWZ{t3gO?x𠗗W߾}c0F5ad̘1cٲeϟ߳gO```>}vڥ룢6o|ӧGGG0 ӡCalܹܿsw1 yyy}*jɒ%sMHHtbxڴis7iii}y&>}#bbbLy)٬j ȑ#7mN)Gϟ?vZ.^yyy... $%%͝;w2ݻs޽{ǎ)b R^6'FC ԀqS\<==쬬\G >/IiiiGYlT*=zӧu[s +t:#ץKbL&aǏߣGD"Ja RD")N z}Qdd b<$|5_G^TPPjat:]6mLb2?I,h4٬<<<^ 8N~aÆo X@~[wuu-**t"֭[Ńz*w  Dz͝0Lddmێ; c#<6,U Nx 43 :411qݺuw%ɓǎ˲… q!\9ĉfyɒ%pzׯ_'Θ1c}ѣfŋh4WNOO&ݻwae˖mܸ-[rOh"{{{n77sYuvv|rAA|7wQQQ .,(RW_}y.]P!x(eH/^r>`nȑ .ܺu+0?̙3}}}W\ɓ0f<,Dӿ{{nݺ۱yxx\reҤIyQQQii+WFW( o߾a-Fqݺuǎe2ٙ3gWXqKwIKKKII===ϟ?P(ꈑBO>|IBB³g_CCxc'Nrss䠘[@W\Yd z}~&`0̙3{^^o0F#kZ8ck& V >|ȇp\*XF1xɤh@ pF:TSSsЬ 0۷1o3/Æ!+WRF#0,8f 8(n /*g,_sNNdjpt:h Y /Ϟ=yfMMͧ~ '''O`_`0jjj~7J$|#m!T V++}q:H<]#D"8`0"<la8_XfNV\2{l肂;]vgM"N&чݺuPg$L.|\@ylHP!'X ߅ N:q:w\__/2&更dnnkΝr_MziMZV2"ў-G%t Ѣid)1~cсͰT &O5K'aZ&S÷F$<;9 @I?Us\\qhE e.'?TH|'o .|`\HJeMJ\I㳕O Mx;2(fX5VktuSY࠿0_h6_KJ/37t!y qfhEht-5 BZT SQJTSEh^v zBN'JȀ'VlȌ*H![` 'zѕ!D]3DHVK3Q<BDfdNq0#4 KP q]O-I@CHÇרDWG(D"Qx+Ϗ%DhqH֊V5{ (Ps^HL-mj'H4N8q۷mmmG e w9~!feh'ǺYܾ};//ӳgϞȰoغ u-qEe}۶m=<< ̲o߾kʨ/"NX 7sjz`Kye_/y^KPG[$zR.^X__sEnl{B p8OE@b%_:g(` L㱐l TSS߻wop|Ox,~K@-$BsBی1\k׎x7,b[%nIEGG\pK@8x |- My $Up(qpGѧFK%5i a\-9"#Uޒϙʗ e%U_zLJ!_ @`37YXi袻-Y yO:e0*ePQ < '\E kƛj! GoS W t,D8 ݶ Lm۶m۶dZG셅[C7dj9~ ==+0f|}5q|hm[թS^zQv7. teee...-Y W=`6LQa4'L{K.ݼy3gRRR***F#IIIXR>W0PA!jG="V~<֭0òl\\?52 05 b/0ѕ5΃$XR6`D0@D% .]ossB5@DR`F.rooHHcǎ\Dh4R߿*GGvuرcǎNJ2..uȐ!3 s) <pFٶm[XXxW '9.K8ڵkrrR իWǏ1cxyyA60{a<##|>y[4Xp!8?p!efIIIa._RRRگ_?!*8111 BVoذ!00VT2ޞKa//_bl6T촴˗/o޼T&tԩv޼yGv4MCCC]]w YtZ~wwf( pSxxѫ Q  Cyy9p_@@8o O0޽{cbbƏoq fÇ E fΏ $kyXGw1YYY;vdW^ҥKjjjEE'0'ONHHXr%e7 /|{ aHb1wܚE >L򺻻'd Àrrr=<<&N F`dkkk=}taa{Ǝ\QQymp̛ZnhhH$ڲe^_rرc}||\]]yjz=zǹ21 3bĈ²2^RRRH,|8=%RKmS3>WEN&cʔ)=zWߧOiO (JǏ_Y[[GFF0 22t;vt֍;%deeеk׮]ƍ3bXqvvx"2@emm zwygΜ9;vܰawFdtEEEEEEEEE0&Lܹs۷o cC'J+//@q"WI _<8%fDn/B=_A#*I\^Rx-Go||{g b-JAAAaG>x;w >};::rE:y]va pgELLp, ܇ 899͞= Ds2mmm}||zͥ߿ȑ#JrΜ92,33رc999eeeRTdeM|X? qX5`!X7;w0]b)szJ|6 ƒg9Q#Q Qg\;Tqf`mmM C w:uT^^ e]]X(RGڶm;k,ggg O>d„ QQQĦ[SS3mڴ#F,\/|(J#Luuu .ӧϜ9steZmQQѳg233333sssCCC׭[g6ٳg%D}%%%}WK񦚻FL$!pkD%h!`B%#HD8 .}߾}>1bU7ݜ9s~嗞={nذ~e2ꪪꔔpprllٳgkjjb< vww|ruu58_;˲hApD˻vz^{V#FX~=qӧ4+Hfn׮qC~Ddcc@Կzݕ|ي3f X)DpX.#^4U ˲&ݻwG) e2?Htss׿viP6 C]]]џӧOVTT؀-ƍggg'zX,6VVVFwmkk wddŋe2xOׯVݱcǴilll#"/_vss:t(}e꫟~k # N߭[Ν;[lD-6GK`4>|$$y1ZjFqqnj#$cctttϹAH$߿jj* յk׮~T*+++ׯ_߯_?F0Ç:3RjjjT*UuuJ wppعsÇ!9VVVֶO>&iҥǏjӦM6m C~ ;ھ}]FDDK8Sk˲}vvvAt)55lm7RmR[("FLs>x,i"l0@ Q'.}ce|6˲ҥKGC sȯL&suue?>|xHHHll,2 2 RYYYeeL&.# 8uĈݻ7l 5c7a ǎ+JJR$H';vh4IIIRffVСCXXؔ)S$ɚ5k\]]###Mv֭-[}۷/ޠh4LM <Z q@L #9!n2  "e++@x˲l߾}o>h \ ׯ6… ӦMK?q>#+@D>ӸBHf-9daRRR&O  ڵkQQQ1PRX5kVbb"WE"x=H弶f͚+Vd2[=QF9::r6v1c0 c4T kk.]XtAWX^4iFJ,|L8Q$]p,为ڴi.^`0͛7`0444׃7CCC;uzի>}ڹs!C 2T qK!a3 s…^{]իVVV7nܰa'|'i)M&Snnn.](!Z Ҕ"^" "F<#7`9!M.kkF.T*0' V8:{l׮]E"O?~z\ꕟ_=Ae{իM&ŋ'LPUU*RHKKVTT*R9eDgfGnZ\0͆ ܒ\`^z :tРA...K.8p`ƍGq8R$9RP+9|puuիWAI\]]={:5o!B.E/ )0666?X+,,e^z]~=88 , Bu 37W/0 ?߸qcڴiSNǍz믿3""G...jg}ֶm[%&<<!p+##㫯R_}UxxH$zUݗ-[f|uwe߿JJ,fs^^/\ׇ+"""$$gȑ#`Mt7???''Gq/(UV=~xܹO<{RiVV'x3fӧOu:hD"R c"Ʒ%DQA Y5666666x{_/ )S~&+V˖-ާ 7n *(((((,t{ܹ֭[cƌ1)));w_1o>}paÆ榦8q*,,ԩSo&iȐ!@`66O8{pOi/^d-F#VQ@Q"ȥ|o6}mݺu 8:e333XVVF|@=<|)4 'x U<>$|!Hp_J}Rv,YwO™bǎxbQm0***>}h삂Xjƌ 5W!'ϗ8lFCo[rurå'?-Z ED 3~MR(Jnm1 Hxw!..zm $~\E :tѣFj۶`޽{jjjJJJVVZرc.]ϟ߮];'''eׯ_|rd{\C`^/**:tЛogϞٳg#[Bo߾m*Yf$3Fمǔ2N+m!OSIx 9a=_dqR{Eaӌ1hͦH$:u/p̙۷t4[2,88xѢE۶mkhh(|@:utE #p/< wQ |o,k46mڔQƄ"DdDׅ  _~k׮9r$ (++S.Ϟ=z"$Qtդ$wwwJ%0^ c|B(q"Y489 3XxV ZXΝ;[lۈϯ7§>}|w;v4{r0DڶmM!ȏte`+jā(Bǯ}#=让Έ5|WH\oQJ8"VF(H,a4|] p> D"l5̢m ATx{{#+I#GrgS@8/ Ìaĉ`tWWWD/l%"D] @b(lH>1LL/r0OH*! &X6χ7%ܸqG^#r0Fؠbv'0 ^k(*p9x!CZQ%ĝ" i>=cW-X@H 'i쵡CGx@!6}.,pPz$8% W)|H>B2Z n_+PHI|q㑖QAdm@tr $Z.i|OU#Kt>Wp$x#Hqr~WNL;ti|D1ϳDP$S*6r.xBWX߼yÇDf<~'+1# 1QH1hRb3+`sns㨦OC,ĿϕZOjv^E"ٳg|%0|B!NM NM,-rnOSw޽rVYlO T2 prrrwwe$r a#֥KJUYY N<ŋ @"G9t&-B)ļB | G7R;S/:kD_$@<|%#s X:@./'DV > HEHTD`-8;\$!<N.m{q|Ha!)ى Qx ͒ZP,5.^^-ꢀpr,T>u9 6zBeKL8jeHkqt { H8M]fcxG‡tx@?qɠXka|@ܺf_bO4.|l >x_rOJ|B9F[T]BaVqE]~37s""X^@$?x~BZ"j7;( ~6)K`[L-"'RGקּ|ɕcؙ36/hь02䛈ҧtm6 q2Lo(XҌ"ںdE _HMlj[*-Nqi[\ {iV= I,(p՗bFsB ]HaQ)Č|qhqk+D;x/=QH—^@pPPjaM7ޢ(F0@D.!( |BN߉JRo w} |B^o2z>oDgk0 }NȲlAAA=ݻ>|믿VTw"AR aV*Z9@CBB0ՕsѩFR a6 YYY;v¬j;t aʔ)K. 83?)oV|||vv6W*Ӄ) ԰0uww![,D:={, @ @BaooϝL|gggn0:/۵kG6>rBZ>x`RRǓg!lٳgcbb_F#G֮]g "<99yΜ9HLL|aBrTRR2i$3fP8a"{X#0Yfeff%p 8fX/^|!fy޼yO<(ӧOSӲeJl΁?`Is qٝwעV!QS&.]QKђV<痽[B ltlIӸrwYuH".M`3.$O#H$/g7ntq[lyxŊH]/ /+HE`0u떑akk+z}@@۷uۀ~A޽qeܹSPP={zjeeebb"dBu=%%s휊GeeeFFFҽAg0tVVV7nptttvvH$~ A$D"ŗ/_0FY~}xx8̇E$길gggHRRٶm[faÆ}jkklmmrСC]]] o`mɒ%mڴK.//D'N}0 ÈD p9KRlll&O,xYӗq^c'K4 ۶m3 O#H1Lqqq;v8&oٲE[4L Vy @ٜ믿[4eل~b j‰ϥĿǎ"977ݻ`Nxnn{8 Pܻwo׮]B(,,wޞ={X$7@III;wr&&&ܹb3w<))iǎ i9]v544_zO>۹sg}}}UUU\\w~-Q$i4]VWW_pn^Ϟ=Kg6 wﮭ;u%$^gϞLl4sCCCll,n6ۧRZÇ钛JD>x ^jG]|YT޸qC'ŸT*t朜cǎTǏc($/͠ǏjkkG}zÃ\WWwqJ%zwߵ瓬bccKKK/>fR"J?~Eln:f̘,X"!;ΝdgsPPо}uUeY6((:t޽;Œ ڻwohhhpp0X"cJߐ={DDDp,qNvvӷo_"@?ų;::fee1 VgΜiccChooc43Z3wZ8^D"\./--h4ӧOCBd*JT644L6ȑL&.))1Lo񆃃T*h4yyyf7prr஖J:.;;e٩S+ɸI'947nPD{u E||@+Weҋ/ L^QQqYZJ̙3]Thӧd7gΜQ*;wB3˲ϟ///(]wʕ"<1!!@W^ nX^@b7m<v*jE _0X^Uho!@0z-\%T _K6}I/Ca4^6/%Ԓ/{ /D N@UUU"\ܿ?77W$!Jܥjwihh`YX޿????777==aXlD*N/))yZ$joj .)| Q㒇L B\___]]B_ډ "99aRaaj+++ZmEE|- h4j:55UV+J>DWC,0pDB6:Kc"/HTfee듒lllDzl@@@rr+RQQQUUSXXheeagg0 vAy CfffeeeEEΝ;/];88<|S$FDD8qƍݺuH$EEE:tppp8sLuuǏϟ?߽{k׮O7t:]iiiQQӧOoܸѮ];?%2[_X ,^&1%/...**)---,,w͞={D"JJ+T*+++sssDӧO#-- _YY ^ VVVo۶ѣG...JRR'OIҚwﺹedd+ǏfffvԩJ >>ݻ:.--ӧ&VVVwܱ3 iiiȖEHALGD(j˖-51cȑ#7o|-aϟȑN:ֺr\ v(rrr*..V(zT.͇H$JKK@m Wd"hѢER{ʲlũ6LN5k x}555 ^_WW䔑j ޽0 |Im tMBnݺ5''aӧ5ܹs7nd&00;x`.]jjj\]]A222ݓmϏa;wxyy%%%߈D#G!!!˗/dVVV?L&xxx80''d2kN,={W_ tvvoWUUچo ڴiSSSx )&&\ܿ ,),;55U$0 뛛o2BCC&eZ6++ Aս{={QN^t)cll,bhhz}~~~uuZm=|ʪ}YYY:us0D*|ĘmeeV\ .߿߱cǽ{e}}}#""eeef8^R EUUZ DDEEEEE1 SUUu6m|7 `RWW^4{ə3g|||233juuuuΝZmnnnXXL&K$[[`(D"i׮]jjꫯZ\\\=(-- LZ~H$߿D"3='!o}}drttl1;c:*++kӦMSgddxzzM&eoE ݤz,pVJ^Uz%s—^jvE?@,[ц!vWD-.q8I$~q"> endobj 322 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure4.png) >> endobj 323 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 324 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 323 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 20005>> stream xyxE8^3ܙ@$p+9uUuA]D||8˗={ MF7|M b]]c bjjoDQ+?h<<tGuVQQѓO>K/UUUuuu)))ruu=|MMVaZ_,[:?.˽***3f̐H$}}}oDZ WX-,,z}[[[~~O>Q555aAlllĂ_Wkk ޼y8>>_Dx5T4....\xΝaÆ DbpOoootTpv̘1o+pРwy'##ƍOjΝ~7 E.]rʎӧO?|… ΥKjjjƎ;|ބ5kWTT۷/++k̘1vZXX_򗴴欬۷o>|<55+**)))|իΝ|+W_~޽{&L2eJ[[Q^xJP(ZZZ~i^9sʕ+/<}+W^rS0 Rx`޼y,do޼ISdW^`^<>uuu===ATN6 UbX[[wޙ;wnBB#00:cƌ+WÑe˖M6mʕ?3<&HIFF` B3<GJKK9kkkj ѣFϺv-Jy5R)7fTTg}W_(ŋ֬Y#JSRR6mdeeuԩ^{WbŊ _Ν_gff>͓d{ꩧ{=H_SS#---p)66v˖-9993!ݻ.]*7l؀?___hH I`4YdD8UYYP( ;bss]P&*QVwttA%M4g}ϯ833s騶+V7o޼/eRRܹs A #){!Hd 8``e``a+;vSؼyk׊,--?Ç3fŊ~_pd2Yhh(7J$AAA666~&>>~Ϟ=۶mh4s3f q#FHLL<{̙3 <<<,--%[om۶#G/^sN//QF.YdG]b;::N2wߍ0aqUUUP;w5j$󳳳srr8.<<|ʔ)_~U`_P_P&6zh>֭ƢAjv޽}A|駃7nheefD%D^awwwo  iɒ%|mll`mFQ.A:Saoo@ "ARd%ZL޼yرc_}: _j޽{W-)QnOOBJ,E]T=ٙ#FP(ׯ^hj%v+hXc4\QL*"C&4w04Z555.]|[[[QZFb|)b1fcE.TnJ|*@dx2(j7dc5!**ƍ ߇T0 45j#&jj&#(4CU+5UQ)Zc{{{CBBlll ZffΝ;Hrl gTZ_$иĨϻO2C70L5662"(I$$H^߹smT**!勊,,,֮]wޞ!sZZZjjj09DjNHׯ[[[\~}dǎT㤂v{ U vvv]]]sˋ=3`;/^4(?WX!˩7nhiiY`hJe^^^^^^aaaCCիWϟ띝BCCCBBBCC---?Cn`7n;v믿C{K_W_}奥8::FEE655XZZ?~*//복 *J&޻w?$$dܸqǏ=z֭[CBB-Zh:;;*+++*****jkk333]]]9s愇S_b8۴iSee_|N"7 qN"ꫯYn.tb%̘SxCgN`#JbEHO2pd׮]]]]xi4gee*Fႂ &9r:44t֬Y3g TW\ioo={6Z`QwOBuJJa')F-1b2ױq2"bvQȒPdل&''߻w`05*""bԨQ[n9s%Kz7#!!! |[Ռ3/^B@`ttNknnOZ^iӦ*XӦM+,,T*֍p-T*ZɓIbҍ2^ g_/0RJ`-J !PsԺǺÙQTmmm؞5F-3ܹsg%ܹsÇK>&""b…(WWWf̘d8qEGG̙3  YKK˨ȷ~/o=zoڵ>>>򩧞6l`裏,,,^~e2#677_r _8Fbrb{ڡTԛܷ25]ͤ]$Byyccc;ZQ|EkDՖ޼y͛%%%nnn111sݵkSLL̳>+d 3r;vHAE GU]]sN*T*ݱc#ǰR(@)rL#hb&6yrTUDI QY OLHPtI^yJ'U f;WTT455ϟ6mڶmt:ڵk]vO?tٲepDS,1]%ٳ?Z-)bŝ|8oy£Z9f '|uI\ʏy…c/fA0R4** PRRnݺ .xxxU"C٭_^ !oRY`AAA\U{xxYZZJ"d x]í ~?ʲ0Td/3}:ÞefW !?9{}q7npww ڲe˭[ dVT&''9sf…2ĉUUUMMMOO_> zp۷og}rS"00pݺu^oooBB^<>|W5ӧ3 ͓ WFEEM<"hZ++nxK88F1PGH&T Q!H v!<8Uz'Ϟ=HIIR1clhT{nMMMllU>3ĄRbMeóg&''K?O|?񏮮/^8`0{Prss\+H6lؠ +G{{XTk.>'˗c&sd|9<(DRWW /=ztѢE؟a*H4*B= uPrP.!0[og c9`着_GQ^^p}͛I㜜klmmớ: boMOO7oޞ={BBB!#F0!L&O>=))_~뭷$D"ill{=5IjDPev|Ȧ&8ncc8~M2Jq3 11hH PB<#P_.8~|ŵpDT޻w/&&2`ƍ...V<^^^pE &@^IHHz믿饗㸸877W_}Uiii<F=.Ċ %C?'\x͛7 }SSS]]][[[oo/GD;v D M1i'l.nbIRq* 5*ehd2ѣ9 {%I||<8 Gŋo߾ R728ȑ#3gΔJىAAAXY!rss\-)d$P"cd9{)))݃[o_@c$ Q\I@Ʌ CakN YFĤTr>Ǐ)!e&#aܹ؟cJo8nŅ 3fԩSoܸacc3{`FQӟ$ ?B-L3ňʊ w5uT1XdF 5TL9!􊊊_~ޞ̖~#Glٲ?`( fggtMe\CD2mڴɓ''LⓋU*›IϽ{:88p7g771c,[,""^/b#3R,ʄA6ـ+kkիW'ȵNiӦ`hB~MHHbW111 ƁI P(EEE=:(l9RUU닮&LP(_>ydNf͚_]?oR)b_R̋bKm󅅅nnnʘI^rbh܆حguuuEEEgg'+ :rӧ=zrGyAm_ jdr F?}.++xf?J>)vI*y(97|hnG.!7mooOLLtvv>y$#?UYYh"3DOŐtww8q"&&<;"A|y7n_~…W^d[f&}F`2&V6ki:;;߿?,,lٲenq+ح|&ĬXͻ0 `޼y(!:^_^^w^LАP(Gvvvvvv?`q]]]?c||g}@*(b"BNyxR6lPYYyС04IH-]&Cf2%&iݸ>bl9请tr>3$g >aggߌ'DN\ZZzy湹hjugg'RՅ;?IFcKKKVVVccի}__@>===ɾ Nh4mmmmmm---jZVxzz޿3mB_.ɔJeDD>k&+q'Oܺu5kvލEiEM>,[hJH%!qRT&Fwliiqٳy;1e ٳ׮]ۺu2z'++AAAV 'J-,,,,,#$NNN^^^ӧOOOOtuu]f &'//ڵk7o8n3f̘4i\TQ[[[]] ammm4+++ CkkZh4r+::zҥɰ`S8j  5~ ^o4R}}}{{{Q/a ؜ \lvmiiO]… /Ud#XjP&"r&K-d* 0|ddsӃC0B+Ü9sz=@T!D{ u:Mpp0|eܹnnnj'u3`4]v9R]?vym޼59288811oWyYjuv???4BQZZ`mm-˭zzz{{{]]]<==JF222xK.wPIw5۶mr@;YuԔ}ÜFu6؂Rq0jT=quO<Uon?l"&&Vy  ;wnPP7[=//_c"ѡR?$&&Bf̘§Նg7ˈ#ÇuuuG}}}1OOOWWWXDcǎ7xQxXtǏ_VV+SN(@xig ``y1۩Q3BaՉ s Փ: RDՙ`Rv ǥJ1$$$$$dڴi/4Fkaa[o>}r'liiٱc뽼;vɌM5G?4iƍ &L;1f#dDkhhP(NNNs28 Em޼Y*7i8͓Tn$˃"T q bIyW"899}waaaRKfìY6nhggd|lA"1zzz.\?Ο?Zeee%%%0uV&mذ!))7߄30 @(-- I04th4c0zzzryMM |ڕ!iӦ nDF: !*r'2Tel3;!d$J$8|@CCv6 9r^?~~Ietׯ_?rHQQQBB͛###uG34wss[f |©@yyyiiimm%0bĈ?|P޻woܹ7oބ/-///..vvvMKK3RGGGW?]"<lI`)D9D"sss>ccc EGG_VDN>ekk7?><<#D"W1dPTTt_~eԩSa O\\\K#``8suu}!Tl0777::ZT/z {JS*p0))СC|I0rAwfl+3F "֊%GN޽{:$$T__?bĈ|%==}֭&M"NRj rJhhFٳgσR#&L0nܸ#G!ìP( FǏ={_O+QB;s SFi f#)SL@%[Ԓ 9S*---ϟ֭CovJ$ꉙ+B(c^S?$vF0򊌌,,,3 eəÆ .tssOq $...##CP/8R(ɥF1,, nԨQ'N3gΜ8qϟ9s ۯ5z ^&cH/9 :uBv  000TꆍW:ǜ B5#V9m ?%#mll=Pl&Ãz^Frtt/rrr>\RR 6a„;w޺ugٻwoppfy!Z " `z05e1r>R RFL1JꃒP4ucU:.Fh^z(D??Q!!!7n܀&ㇹF'@.?~ܸq<''端ڱcǰavVFMPAK2ߩb1GQ9cIqSBYVy?9R*-=b ~,ũja>69;;1cDGGϜ9_~eO?>-TeH̭lB1B9@ YI%%L&rww.vɃ(5wy!w]Շ "N3"#(gxVTO$V(S* Wtv*Y77SNYZZ*7| ejЌ k@&C;U:RGJ(@^"\t ^xd6P+U(j@Ѩ`mm oƘ6EM,IHFBxvQa„ gMcQeW,J4=H@irPhYJ0Ȗ&$ц w̙3ȑ#cƌBqDҤ#Ft:E &-$Kd;h ҉1>t萫kss3#d)恘$ATFP[D!zFlmm` '3`ܸq$?CC1L$i۠l7{0zxjFcggo(!ܘ`3+i  OӋ9%ҫh)HG@a3P\.߳g)C`!QEl$ЕRj2y)K &_2&ԆKR pEn8H?O֣a z,S+D L,@ؒߤBI&D`擦"`f-$lSR7/TH Z;vMS q ։mǥw{]H^gIBR+Bm9@9S0Hq(C14* Uy1+C[!Θ4fhԈ)&P^7@f`# fJZu< lܯj$\IdCI8%\&,ɧ9-- >09)ƁD,gԶImdfDP0 MIm/_,;;`PE6fE(~ xY``sb~'[,%'I*m)kjju֝;w좚#d2v,T( 4aBʋ1=f)7pfDp9!v}뫪"]L=f` ]$ B"N[+["G8q"|ԢE===T"a(c2 qbz[[!UEqR" $H${{ qF 'P6C13 @lvttX  P'x*aHC%HA߶F*ʑ#GB^xBb9 ~׸eHA3%; ŋc`n& ](. $|&?Q!'d2CЭb6-73@t8@L1oAtvȚ7%"3B̮R`et !hWܶcq!uM95-!y<H*mgmЋ\DPXQiGP|hLJVqE-Nqn@6ֹ4%_BU D5 nFΠҊ1*K0D!)& c )naPHBG @`cP-'Ѱet&3"#*K=nSE`&01/j8R:ϟ]TyndԨ> [C-Uy1"ڢdYr ?nE ۰q,lF&JB&ZhԆ` -(C !8hm\ؒQ#S L%gT8AT :ر OZ`-I(RIlRg(ժYa2*l*3i2V!l&hB9" e@u] * 3V1Q QXR"(Ft yA *uUA$h Qd+S& l>)1 o+!&"@6AC"xkB*.\)v ]i6°fBΒK!#FS j(D5DL<`o<}T Fu# 砨L:Ƚ*1-6()cJAܶ$f߂96Cy0ؠ𝞁/A5ݴǻ GY?NR__ѣGcS"gRῒ U}z`i|e_F8U.=" j| _BĐerxsH)C.Y$55u$11СC  Zjڴi=T}}}QQQWWBVקj~KcwпF qpp())# .uppXx1Cܞ~iNrry87n^v-իWf\rbधWVV //`TVV~7۷og}w[ne8p`ڴi! ۷o=3ǝ9sf˖- BRZV-))6V|(?Ν; 3g8pΝ;TArC|{U555H0 ŗ/_>uOKWT={CUUŋ<($O>!?<88 z}bbbQQѸq$6E<ܨ( !4!&)))ƍc 7|k4mllK{{{x7^&NF KKKGGǴ*hJ...---Æ #q8SZÇ^^^T0iҤ:,Jjhhprr1birPTAAAF"dff7.55IGz(~!_D봴41 Q===ǎ+,,5 ٚSAP޽wa }7 z}{{}%%%z+ CGGh4Bax>z`0PqڒP^O ֶo>Ңjlnnvڑ#GJ%#|R3?@Np]// X[[ۃ_ i?]\\M6zh*D$-;;{!!!٪ݹsg9r$[C*y<9NB?>55]]]NNNZΝ}v{{?Vp¹sܹ#!.ct:_]]M5D_x>>>0 ҥKΝ+))`4322Ο?&oooxꩧ^{5Ta2LJ$L&JϞ=xblz5v}Lhmtqpp8uI؈`;::yZ1رcuuuOFB EC8uRΝI3?սth׿vmN7aggS ޽{233 ĉrQF~?I&Ϛ5 ˟}4t111vvvbmmm%&&6..}ǃzСckk qP>tIHHѣGN:mwppHHH 3wޕH$/_NdD7VQy&.\pvvwoֆjSM]VrwwX!=IIIuuu-))ydB___JJ[qq /ovzX՞9sF*:;;M%%`0)D5Luj%,J_}Uf HR{{{//^x߆aBϟO0ܡ:;;Sd0"""Lot8.88Xь?>** *;u@t4lذ'|R.2aaa3cǎŤ222?Wɔ|N۷s̱ԕ{g0ZXb Cz)\q=<<ȭ Մ~ڴi'N  N<9~0ZD$3gBBBƎ[__?f̘1cyWrFCXtiII %~UVVzzzZYY yrѢEMMM| ,Lz̙3Gt:F6iqqqRzM̙33 !0OIcbbBBB[&Q,V3w܀ W lrM:ttt|wgmgg}ƍ@ln[c D ? ٧[ظHRq"#Fj#QYr0*a|H !Hء-ӤQh7' DLmqG P̋gT:-T#sQ y$C1IBc8ȯ_Pdbd(!&5y&!` uq 0(իEI$"Id 7Hk#9_~C#8,_-R[ѯeojjj<8>xUT*JT2XtttC tn8`0ܽ{6??㸚'Aףvuttdgg?|H]@TƜFu/Q4pclmm7.C䮮.Nq\CCCvvvnn.s~{{{__dCooJ"7oh4';;8z?*q.ꦦ>`twwcGR#w ]#6m[{V6/Էkkk Saa!2l߾}<0a|Kcccee{322jkk;;; &JyA------~+W[ Eee%<%%eĉ)))~~~cǎqFkkkXX\.OOOoiiqss+((?yq㲳}}}o޼P(oݺ9  X60p0!qTZlkk+//U*]AFG5jmVVF)))qpp(..6 A[n;aaa^#X]] veee{xxH$ӧOO:~rrrʂ߿:22~...̌~{NN^ijjh4* ޶>|p,dx {G%e*{?ؿii)g>}zFFƾ}_'NX]]cmmmmm$,,,T*UsssGGG[[T* tˬe2`ٲe!!!RRxÔD"quutR||ɓ'}||Fc``T*겱1 555>>>ӧO/))섍VBlM:PB\!I)DZ/^p0nܸw}W&{2L&T*ӦMjժ;wz@MMȑ#KJJjCxxx___```AAANjggw۲e!T*urrtAAAEEEqqqݩ򊇇Ggggjj9l[[[lmmcǎU:~xx/| DxOnQhA?s}WyYfErwwW*naÆuuu)fX_un^x>wqرc/\骫ju[[N Q(666α< 1 NNN&::g͚vyF[3rtҥKjunn#GYWWn/// L]*CBB|}}%/pvvkXZZbƔw8qm-888--㸪*Zhmll`GQSS~](E.`$$$\|9""{Z s|p08|H8Fov2!j{-D"πzzzb&"@vz`߱V\\lii9uTT$ ᐟU*-^g*&ޤB(>OB3Tg،IPМmH`9Lz \Y[ c5&1cP|AߨXMTMfl1UٻYzB"{" $OK7cJԠ $lMnٟb I%d :.^ endstream endobj 325 0 obj << /Type /Annot /Subtype /Link /A 326 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 28.4528 260.2500 197.9528 ] >> endobj 326 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure5.png) >> endobj 327 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 328 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 327 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 41263>> stream xw\?>z4!"؈%h4[,c4hhQ4*(" (H* swݳzy<<>gv8q "`xȁTP)&&A$ d~&X4&8,)QQuZ ".}TTvuuADbV[[[^^TTT~7^vfmmm-aRdeeeuuuPgϚ` N8!/\p}΍7‡+WdggGDDǎST\z/111&&4..cΝkuuL&njj*--Qmmm*z2憆7n|$|W"h۶mǏbqCCaRYYYQQQgggkk+4^$AW777K$,TaX,.**ڰaL|rXT666fgg+ LVUU^__P(Riuuu[[aXgggii)Drfav`յi& `ɁC| sܼ\T{xxx?sذa<=|P"ttt؜;wӣG^r~xxxpp^[[{-PW_|CCëWvww:uJP:tHKK;rHKK=<<>SJ驣3uT>ogggkk{O?444tĉeeeмׯǏ뭷v5a„ѕuuul f̘T*#""N< ‹/zzzܹ˞}D"x񢏏ObbbZZZ|||eee``Rwɓ+++ry[[˗-,,rrrlmmmmmR-yB%|SqqqKK˄ >}JKK9RK,--kjjpJϟё[WWwT@QQLP(233߿eff{YfY[[>|3!!811,44СC&MڳgϿ|rPPr͛77nSRRf̘i&++7oz{{:u ðð77gd_UT&&&~_f ;w… 555Ϟ=kggWSScbbRVV[Ž_~ӧeee~~~aaa&M"gXɝ,%JR鵵hX[[[[[Rm۶gϞQF988m޼y֬Y׮]`dd~zboo޼lڵkYYYuuuݳܼy3:::g͚E?!!)<<޽{*ߟ pŊ۶m333[vrqqqqq9tʕ[n޽?iF5{l̙3̙󎎎Jcddm۶s疗[B?0455sKKKmmmSS СCAAAo֡C~嗳gLX222 E__ 477_?Nbf\WWf͛wÛ7o̙C <<<֯_f͚5k_v!//e˖}eeecǎz~> jkk͇ F=zڵk?裥K>{ رcɒ%+VzG222n߾qƑ#GB6///'''gg˗=ȨXx-[ꈐ~{֭555?;w͛]]]kkkuuuy<^__L&ѣW~ ""⧟~R*<t8kxCT:i$49mڴ[nwvv%%%Æ +((x7[ZZ Ecc#FyDS^>Oncǎ駟(ux={̙C6#y/aNNN˖-~Ç_0h'ɓG #RH155nhh:::Ӄᳱ͛e˖ӧGGGO>6U*)@{{{{{ಲ8w܈2É,4FFFz+++O0!%%:qds1bDjjj}}=& ύjjj/_P(oNʇ;a„'Oܹ355իJ\ 9| OOO孭0DWWB )3&MtǏG 8 :@@7=%3 g͚аo߾;w={ 2d͚5;w477O<<<:::BqLJ r۷[[[Cfmll͛wݻwwuu]r,11qnnnWWӧO_paٲe<{{{ݝɓYfFDDlڴRtӦM7n,--aO8d}}}ǧMsN ChiiA8C Ԅ!zzz6lrСCq8[[[΄nڴ)))iŇR(>ӦMCze겴D jhhoڴ zaÆEEEccc?##9**jҥЁC ?|pxx+B)""nǎ˖-KNN311ٸqcMMMUUĉ! rJ$--9;;'AAAVVVv  E~~>7-,, baaaTB啒sN>:;;wttXXX@3z'|rС'n߾=00J&YXXPq*Fg괁dJLLܾ};(ʇM)GSY -@v?Í7~'OK.D"Z{GK0x<8?"E S$&-@۶mKII?; y뭷4PP̛7j'L*(h": ͋Aw RZ[[?3naa1n8'y{{;::R %_[/w u*HOO7 hjj0񣶱M>ÇsT?Hğۃe7bJ Z؉_"`I(DRRuꔯi+**zzz<<<˟?T*wQ[[+ &L뫧-"@M;j mc t%pQ0Dd٫e*bb#J $kGd~5B(QhҢh@YYY9U*UVVVLL̽{gϞ} GGGRW_-^(ʊGikkM0aܸq555˖- jR,La@4{/x _ٵpB1E A=,i `%y_5`{{'OƏ/ݻoرА6j(zj :;;322=ztԩ6??_ĉs555522b;==($$}̝;/΅)yozɓ'oF,O2eݺucƌ!NH|>l̙3gq\P(!!!%%E$b' -,,,--yyy_~ezzzJJ <|||&O<$OPzqooo@@-,,cf q7w)225qDcccr,UUUFFFBG*3D0 [t)H$R(>T[455Nk.GG˗/kkka*J,Ꚙ?~ҤI#Fe*3T )A%%%ǎnܸ9zLݻ+KCL~O/Ř3gdggl㸎T*2eӧO{~mff&ඞR"""V^Maq4>>>!!m„ 3gljj:r\.߶m$(Zfff.f92##(x@ ͉ ѿL&{.>>ZZZWWk a }ĉB011deeI$T?WHH9 .8fZtTkh4Vm~DW )..O0!66VSS-XB###333u'O 2$$$ĖF@XXؒ%KYrb&R۪Λ7ʕ+SN533xw?}4///!!y8lذ3g]xqd"mmmOOO9͛7̙3o&L,J\]]KJJF xxxP*!C0d~49SôI a;n j ,x+``CCüy󴴴*++===!/Q!i=FV?Ϛ5ҥK---iiiJrʔ)+V8zNXkk7xC-dH6i$ڶlOKKKJJ4/dz6mqb\.?z@ ҊYh.j-!p@%OQ o[pܻw=##q8:9J.. TA{♩(Ȳb!ç'B(+ oܸ{4r\T*MMM> O$Dhhhxī)#'SM @KKsΑٸ7hqmm%K޽ѣ&MڰaJPB[[{СwXҚAG=㸻{GG\Z R) D 0̃ZLKj]LT@J<,D>ej8əd)vuu}!<!0,444 ŋO8ex;@BQ ?(\^QQC@Bwڵ^^^_uFFƌ3v=u^1xOoGGEGbOhp.Li!miUe)D٩T*Ҧe;v?:JyyyfffxvLSXXh``$ѡT*ՖOs̙3gBcҤI6lV.vh>NG2LWW@KŦQ5zQdd2@V&GSkjj233޽KB</!!^HI8Ϗbرc0ٳgϞ={IOO5k?S @ZP3ׯg6$`9x!L_(@2_ǎ@P CCCQHKKɉ|&:#?~޽>H[[I|upp l߾}ĵ% / b'0 68q"22 #jTD/^=MMMǏ/))E*\Aj(vttJE2J._+w+ׯ_W(,i)%㵶cuWu m(puNG.I$FE"Qii,EZ=@ P*Օ555$333 p?qĹs|}}'LL‰g"˸X58i ̟?;rȹs8hѢ5kXZZҖSN͛7t 4'$$7bĈ x3A6YD P%4LNȁ(ܡ6FP(-4 疖&LXhTGzxxϝ;O* B'''xQEE_=jԨ B}:''5 ERʈKi픨*0͛/_ѹr__ߣGd2ˉ=&qvv5jԌ3LMM'X|ҥ zzznٲeȑJL՛.#F0MMMgϞݰaQFF… 2)UR(.ޒCrTɯe%2 A .QS'NheeC=zo_7n\VVָqp!Q00--ðkΜ9xjȐ!SN]j<(((00ַϟGDDܹSSSs̘1bXSSsϞ=WvuuH$}}}===3ZRR2fDb 777---mmmmmmMMM]]] ===@ܹP(6l؀g񏁁 ֭[|AH~P\80CKNN퍋;+**||| ύ7<8m4b*L! z$SI7FbD+$ʆ d={rV\PP,--$I~~~UU QTB[IhKVVV~R? ~뭷ZRZ[j> D'NZXX|7...p _OO@OOVTܼp¡C»e2\.J CRTQFiii566Һ6/Ȝ|>?88xէN:tмy֮]kmm]RR% )]ѿ_q:7nܨ B?~,cbbˉ@~¿jjj?~ٳ[nW_ݽ{^1ӧ γg.ZwީT߼y|g egg/]tӦM~~~IJQ]]sى'.^8///**޽{2:;;9;;ÐqVk $쩘:WMz$T*mii!&0`ƃ~]kڵp6LT^y.10jժU}}}7nI,002rϙ3G*]6/// !H i:=eToPEEŵk׺)Y pb203Ԧz)FP\Ryd999,lBjkk;ONRPPp֭7jii(8Cb:88Q444)-@J8 UTTX,^lYWWoYCNJ?wQ8?gu'^탂Mprrrљ9s&|%'N ISSs۶m3xѢE/]4{ӧOԅ L6mС ¼lbBPBiQf0v WPB.&bj$Pd%9@S.iaݻw$W;q0X_^z՗/_vwwG bTg/NlJ577(ai--*!gKIIImmmGGRfLCླྀ#-䴀8'6oW5%L0Ph@M((=yyyX)-d}VVʕ+CBB$) @&YYYy r>55F;AOO?#L"7&&ٳ~B b~`ff& M033kll"R(xZC;h2ɧPlf1b,rg٢mk)(ZLMMMܹ# *Ǣ@DyU*[nݰaB=nџ֙䴖_#WG͝;əL9hnn~U\vtt(CCCOO7|#!CKV.W*}aRloo ?٩Vbo=hnnbhhhddqH<ƍϟi G1 􌈈 .422600:::?c~+*TJ% yX===AOO4~>oiӦM=ta@0?~ w XXXXMMM H-}H;|' $$9VQ`jدHHUt;@&_Q;v 2QE\|^@JfEiy(*D7o @>Ci'0a„?XWWhB~ $bb K&2 .|*Ck YPJV3UN!hm۶m̙D%0k"H)TKvJ,dDz|vvvvww'/4fӧ듷q\av?$q11%%%-"bKȥp1Pڵ ^$‘::bĈ\b%^(OOF6+W֭[/{ӺKRՒWLLLmm-i $@v/( X @wwwyyyll,GKLVQT*JVt hZOˉG!^,X ) id!|wL&ɲc}YhI, E]^U4W BJUVV6t~'_)FGuuuRRDM'˙qtۯ +ד.8??޽{D emlkJ0JuƍtɎRL&06xeY`7һ%*55uLzi!WTTܻwc_b!vs]8G(C )--eee2J[N<_ڶmAbv\wׯ7n\HHȆ s%%%UTT RvvG}TQQwߡ]4dRrA;gΜ WJ+&GJJNNϷl幹cƌ8^XXH|CR269MřR4/bu^^Y__@./2yyy7o$wf y…20#DH$'NߴiSddH$Zp޽{![ggguuuMMMuu5\$d]]]C?H@P,el$L!2YZQ) uqq1ϯ9T*)K?NE?HL&EpZL6Ms[xv6H4d MMM2%;;{̘10}HHƭ[X[IOE >%\o-[ eMKK{ȑ# fSSS#GBGrrqmmmܹ3|y-\Ϗ|:ܹsҥK EZ꠵׮]+**ruu/f uGAJDڙ3gΚ5 qX\SSTTT4ydwߕY@ DDRui~u#[EAAimj vZ h9(v;__YfO2ngȢȁMMMEEE:-[<<?gΜ[[)S0 (ȯܧH;1A(؝ZBA>Z,8 lnStƍ?XSS~prr&w>(W_}5zhaXllbccF_(^0,99ٳeeeK."8?04iҙ3g6o|…C\vmǎ#G\`iYRU1vww;v 4zhx3=&b{Jox{{}ebzFPt,(ru :PifffvvvWnkk6l߸qF U*U|||vvD"MJJ\]]/]D>qOOOٳoܸ1f̘m۶gٳg/_~̙SN:ð'O\vm߾}...'O177?gaaaAAAqqqSSעE<==---1 ;{lddܹswܙѣGO2 A4=ϟ?d߃aիWG}СP.U%˅e/R[rl?fߺuTˉaXDDDqq_~aXYYٓ'O222rrr0 wwwmiiصk䢢Ǐ'&&\r…oZR*bG@yy{QfH?ӧy<Ν;E"7o޸qѣ)d2YfffRRRJJX,rرk#?&'''''j˖-&&&111 iٲe{}wnM܉>!L"&O /T*-..|kjj,,,V\+++}}}===>}SZ핕C c)T*qQQQoON[ҥKÆ 2d"?TOO` 7]]]3g+)@jjj(\J577',D)fffׯ_wbMpc!bbbbBCCqӳtqqT` Μ9=4&L(,,g1|U*yDhBB[m4RۭYOx*RvH/ }MII INN"<"A:u*vrR!!!V***'dS600rʕ+by<)7N(444%%78vؐ!C/\c %|@ !ڌg$$$KRڂBvj6{]0 miM"ІsH+M;9@&'''CCÒ'p͛i"jhhQE3x޽d%VŴiӖ.]JAkyx<ޅ  k lll F 9s?{o % qc>gΜ'3XZK0rHB3y䚚hѢ;wP 5ZC- 2Ҧa`R;;b{w (kiid2pZՋ/>vdzw!gΜD}YTTԎ;0 ޺ukHHHcc3g"##|Mb0 3gξ}XnKLJ==DFF ~Kҵw\wJ7666}Ņ6  ',#u& #Glݺ766/lmm׭[/Ku58!88;v2ox=Mp1HaCsiSq$ 666<ҥKִ6N`VH$V(|Xo8nccn:&QU$R-dA3AVkKZSPӪd ,,PMdT*KL $+**hdO6-CqqkWWj9# š8`F8^XZ  +S.sHR'Y\vR`m_+? QV3((YZn?|p͚5\6+h &L{I? -   ,mreIr$PtQbXx W&J8 )H:&I& ˗/X$+.Z/xѥ \O |y )( 9 ǘ+$KKvB  MO?D ᘊIE+KGGEt#\ C Zi{-T;"“,{T`&!dQ  vW&72% |fv,f:p\q 9(e :BK{*B]YYYppp^^ĉqqq555qqqĭ+"cc㐐 s\\+7o7Rsq6 _sssꤤ$BF"4yyy9qY&ʏaXIIIzz:0&0֭[ZjhhHHH b?H+',QQQE"QttZtdQ!;77͎Z QQQQb#˗/7xz~Z{n߾܌5 ZZ^^Ύe`_+S`,u .NV̡p/v 0^R +hzhyǴIKd|%Z!\$P%ZDpP=Z ^HU-\BZUY}0LA ހ 'j@JMMmjj"3P@OKKkiihgZZZOOG'OT*(}}},=JKK#!Hh3W,HD"Ǵ ɣR۷9 sG,J233LRRRR8ڀa؉'Gyjb)Yd p iѣGRСCզhnn B aaavvv,z]SSS̽/^U(\߿_^^8dvNHPRR xb^^n*(//?DB)%%ԴёLKKD)))>>> ϙ(,,Ã|-EEE>x`ᔳPzYmmmRR/B>|RTTg`JJH$qss$oAYȤI={e!===ggÇX6113fLJJʨQԚP(?R9 HuuuQQO:;;755蘘3x<''N.f"?~?OyAC x񢾾;T*RkIeeX,GggH$eg655 7n\GGP(L1[(&$$;"蘙9evjƺ2*… 8WVV.4),, ð^v88ye7ٳ{ii-,, 񂂂6f;w0x6B%D"`㖖f%HK9998h8~]ꊊ233U*UrrZ҇b.(*++ S-mm-ӧeΝ;8766#H޽Rz{,ʀxSSS\\\vvӉFoii͝>}5eee3ݺu+'''88X(_f2b~O/vtthkkKNHH(((ʂ/?RABRF1uTuegggeeԌ=ČxNNΓ'OOX奧?|ҤI RSS+**LB\Çϟ?6mțŒ䒒Yf{Dg4>=5 OIIɥKd2ٮ]ݻ"P^^ٹsj'+**.]Դ{nLݗ_~ ^:SP744\t/k'd斖;vk'3K.~gٴ BD"џ}<&KŔYh &&&::: ͭ ׯ3f nܸ;j(2ׯ1߿-n߾}5OOO8D KsNddgHHHQQ;aÆ͞=ӧ### o!Z=!x@7dzEn߾gllaq">$&&(Jsse˖QHҾjW`eh$a">0 _zr"Ceׯ0H|8$Q;}2-?%l!L*Tˠ0,$p :0NbZT KGC R)C-_PX" t"Gd_Yɢ,%ݖ ZM$ %r Y(Of@2xcccbS|ZZիW555nݺmddnggGR U*f2j={DP(PHFFF~~~[[ 99>))k蘔/*999"^(fff_S>L>ddRǔ#,AJT{{{qq1RSSmll\]WWw----Ǐ{%溺:cccmmmNYx8r@0 .**~L&kooҺ}J*))T $%% BR xXа`f!%"RVG-X'!{)++X7UڵkӧO/..vttD:::uuu===r[XXd7o P$ njΒ<396L9m۶I$@CCCccq͛577o-ZTPPlx|>_PX[[+J>/4EPLuV{{{mTTR@"333-ZT__?mmmO:::nnnyyyÇDb\T*kcc@ hnn0L.WWWx)WZ!h }[#ЁJ200H$WWWWT>yn+ՅrnƇ;iG&!MMMzy === USSڵvvvphAARvuud2b' ‘RmmmɻdR311d ӓPfC[BQ__ Z*67}w\TG]veU:"b`}wԠ-ϓĘ1&1k5vaYX?03wR4<393e` vU |Rl4AY[[r^zW8Rj6b?_HRg#dw03rbM 6^G,G'-8>|e. ^%2 x҃F̥ucU#MB.LvW|Ґx!`q ~$#! Qr3 qqL. >NHqէO9;;;::syrrrkkkR)x[]]'J]\\D"QCCÝ;w=$>xf0<==E"V53"*--ljSSS\h|rZZZCCB|}}% xËa\r<0Ʀqp8|kekkkwekkkϜ9@PvvD"jNm èj!N3xSF٦MD,HP8998q"555//M6r<66655533ѱ^ XK0L@@kXXXjj*H߻wo.]ߏ?~\:99gQ>Gx+z~ٲebx֭O}(J#G@욤8Т%gv 7oޜ]VVzk׮999%''oe]8D۷hH$oq)RQYYy]ew'''++KL&W.畕t] Ilŋfsuu^^21cϹs|M{0`@`` 0f/\dӧ2gLL̕+WΜ9#>%KGGG'$$k… g^tI./_|%%%˖-x񢽽W_}e0>S???\խ[˗:twٷo_5==]Vڮ\r۶m'O.++{6ls,˾۶֭AI̜:u}Fh=zT[[jժÇϚ5k^^^ bݺu3gҥK 7lؐeEpW_}~ʕ]tի׀jjjgϞB [KRG*;/00pذaŋzw;v찶~kN7vXy3&!!aǏԩSCCӧO?Cooo`0L4ҥK L0͛ G 6lXhh(˲~!0aaaÆ +))ٹsBG 4rHǬӳgbbb.\q7N0رc C_jJJKKx{I&}aaa3f_8dȐ8p@.k;rHΝ;fmm=|q}:.//رc 9r$::Z$yyy]t 0l0tݪ 2SHuk4'd+>|ӽmڴ9rdquutz~6mXf (۷ɓ'E"QLL̠A駟2 cggWQQqҥq%'';;;K;w,;}t^4iƍ={?1 h _,\pǎJDDį{k?좢>30t_W^=zubb"$l6/_|wᶍZbѣ8O?͜9S2 SYY}A?Ν;/\`0 fΜG1 DfĊW>M:Օ/.\6lÇWZ{t3gO?x𠗗W߾}c0F5ad̘1cٲeϟ߳gO```>}vڥ룢6o|ӧGGG0 ӡCalܹܿsw1 yyy}*jɒ%sMHHtbxڴis7iii}y&>}#bbbLy)٬j ȑ#7mN)Gϟ?vZ.^yyy... $%%͝;w2ݻs޽{ǎ)b R^6'FC ԀqS\<==쬬\G >/IiiiGYlT*=zӧu[s +t:#ץKbL&aǏߣGD"Ja RD")N z}Qdd b<$|5_G^TPPjat:]6mLb2?I,h4٬<<<^ 8N~aÆo X@~[wuu-**t"֭[Ńz*w  Dz͝0Lddmێ; c#<6,U Nx 43 :411qݺuw%ɓǎ˲… q!\9ĉfyɒ%pzׯ_'Θ1c}ѣfŋh4WNOO&ݻwae˖mܸ-[rOh"{{{n77sYuvv|rAA|7wQQQ .,(RW_}y.]P!x(eH/^r>`nȑ .ܺu+0?̙3}}}W\ɓ0f<,Dӿ{{nݺ۱yxx\reҤIyQQQii+WFW( o߾a-Fqݺuǎe2ٙ3gWXqKwIKKKII===ϟ?P(ꈑBO>|IBB³g_CCxc'Nrss䠘[@W\Yd z}~&`0̙3{^^o0F#kZ8ck& V >|ȇp\*XF1xɤh@ pF:TSSsЬ 0۷1o3/Æ!+WRF#0,8f 8(n /*g,_sNNdjpt:h Y /Ϟ=yfMMͧ~ '''O`_`0jjj~7J$|#m!T V++}q:H<]#D"8`0"<la8_XfNV\2{l肂;]vgM"N&чݺuPg$L.|\@ylHP!'X ߅ N:q:w\__/2&更dnnkΝr_MziMZV2"ў-G%t Ѣid)1~cсͰT &O5K'aZ&S÷F$<;9 @I?Us\\qhE e.'?TH|'o .|`\HJeMJ\I㳕O Mx;2(fX5VktuSY࠿0_h6_KJ/37t!y qfhEht-5 BZT SQJTSEh^v zBN'JȀ'VlȌ*H![` 'zѕ!D]3DHVK3Q<BDfdNq0#4 KP q]O-I@CHÇרDWG(D"Qx+Ϗ%DhqH֊V5{ (Ps^HL-mj'H4N8q۷mmmG e w9~!feh'ǺYܾ};//ӳgϞȰoغ u-qEe}۶m=<< ̲o߾kʨ/"NX 7sjz`Kye_/y^KPG[$zR.^X__sEnl{B p8OE@b%_:g(` L㱐l TSS߻wop|Ox,~K@-$BsBی1\k׎x7,b[%nIEGG\pK@8x |- My $Up(qpGѧFK%5i a\-9"#Uޒϙʗ e%U_zLJ!_ @`37YXi袻-Y yO:e0*ePQ < '\E kƛj! GoS W t,D8 ݶ Lm۶m۶dZG셅[C7dj9~ ==+0f|}5q|hm[թS^zQv7. teee...-Y W=`6LQa4'L{K.ݼy3gRRR***F#IIIXR>W0PA!jG="V~<֭0òl\\?52 05 b/0ѕ5΃$XR6`D0@D% .]ossB5@DR`F.rooHHcǎ\Dh4R߿*GGvuرcǎNJ2..uȐ!3 s) <pFٶm[XXxW '9.K8ڵkrrR իWǏ1cxyyA60{a<##|>y[4Xp!8?p!efIIIa._RRRگ_?!*8111 BVoذ!00VT2ޞKa//_bl6T촴˗/o޼T&tԩv޼yGv4MCCC]]w YtZ~wwf( pSxxѫ Q  Cyy9p_@@8o O0޽{cbbƏoq fÇ E fΏ $kyXGw1YYY;vdW^ҥKjjjEE'0'ONHHXr%e7 /|{ aHb1wܚE >L򺻻'd Àrrr=<<&N F`dkkk=}taa{Ǝ\QQymp̛ZnhhH$ڲe^_rرc}||\]]yjz=zǹ21 3bĈ²2^RRRH,|8=%RKmS3>WEN&cʔ)=zWߧOiO (JǏ_Y[[GFF0 22t;vt֍;%deeеk׮]ƍ3bXqvvx"2@emm zwygΜ9;vܰawFdtEEEEEEEEE0&Lܹs۷o cC'J+//@q"WI _<8%fDn/B=_A#*I\^Rx-Go||{g b-JAAAaG>x;w >};::rE:y]va pgELLp, ܇ 899͞= Ds2mmm}||zͥ߿ȑ#JrΜ92,33رc999eeeRTdeM|X? qX5`!X7;w0]b)szJ|6 ƒg9Q#Q Qg\;Tqf`mmM C w:uT^^ e]]X(RGڶm;k,ggg O>d„ QQQĦ[SS3mڴ#F,\/|(J#Luuu .ӧϜ9steZmQQѳg233333sssCCC׭[g6ٳg%D}%%%}WK񦚻FL$!pkD%h!`B%#HD8 .}߾}>1bU7ݜ9s~嗞={nذ~e2ꪪꔔpprllٳgkjjb< vww|ruu58_;˲hApD˻vz^{V#FX~=qӧ4+Hfn׮qC~Ddcc@Կzݕ|ي3f X)DpX.#^4U ˲&ݻwG) e2?Htss׿viP6 C]]]џӧOVTT؀-ƍggg'zX,6VVVFwmkk wddŋe2xOׯVݱcǴilll#"/_vss:t(}e꫟~k # N߭[Ν;[lD-6GK`4>|$$y1ZjFqqnj#$cctttϹAH$߿jj* յk׮~T*+++ׯ_߯_?F0Ç:3RjjjT*UuuJ wppعsÇ!9VVVֶO>&iҥǏjӦM6m C~ ;ھ}]FDDK8Sk˲}vvvAt)55lm7RmR[("FLs>x,i"l0@ Q'.}ce|6˲ҥKGC sȯL&suue?>|xHHHll,2 2 RYYYeeL&.# 8uĈݻ7l 5c7a ǎ+JJR$H';vh4IIIRffVСCXXؔ)S$ɚ5k\]]###Mv֭-[}۷/ޠh4LM <Z q@L #9!n2  "e++@x˲l߾}o>h \ ׯ6… ӦMK?q>#+@D>ӸBHf-9daRRR&O  ڵkQQQ1PRX5kVbb"WE"x=H弶f͚+Vd2[=QF9::r6v1c0 c4T kk.]XtAWX^4iFJ,|L8Q$]p,为ڴi.^`0͛7`0444׃7CCC;uzի>}ڹs!C 2T qK!a3 s…^{]իVVV7nܰa'|'i)M&Snnn.](!Z Ҕ"^" "F<#7`9!M.kkF.T*0' V8:{l׮]E"O?~z\ꕟ_=Ae{իM&ŋ'LPUU*RHKKVTT*R9eDgfGnZ\0͆ ܒ\`^z :tРA...K.8p`ƍGq8R$9RP+9|puuիWAI\]]={:5o!B.E/ )0666?X+,,e^z]~=88 , Bu 37W/0 ?߸qcڴiSNǍz믿3""G...jg}ֶm[%&<<!p+##㫯R_}UxxH$zUݗ-[f|uwe߿JJ,fs^^/\ׇ+"""$$gȑ#`Mt7???''Gq/(UV=~xܹO<{RiVV'x3fӧOu:hD"R c"Ʒ%DQA Y5666666x{_/ )S~&+V˖-ާ 7n *(((((,t{ܹ֭[cƌ1)));w_1o>}paÆ榦8q*,,ԩSo&iȐ!@`66O8{pOi/^d-F#VQ@Q"ȥ|o6}mݺu 8:e333XVVF|@=<|)4 'x U<>$|!Hp_J}Rv,YwO™bǎxbQm0***>}h삂Xjƌ 5W!'ϗ8lFCo[rurå'?-Z ED 3~MR(Jnm1 Hxw!..zm $~\E :tѣFj۶`޽{jjjJJJVVZرc.]ϟ߮];'''eׯ_|rd{\C`^/**:tЛogϞٳg#[Bo߾m*Yf$3Fمǔ2N+m!OSIx 9a=_dqR{Eaӌ1hͦH$:u/p̙۷t4[2,88xѢE۶mkhh(|@:utE #p/< wQ |o,k46mڔQƄ"DdDׅ  _~k׮9r$ (++S.Ϟ=z"$Qtդ$wwwJ%0^ c|B(q"Y489 3XxV ZXΝ;[lۈϯ7§>}|w;v4{r0DڶmM!ȏte`+jā(Bǯ}#=让Έ5|WH\oQJ8"VF(H,a4|] p> D"l5̢m ATx{{#+I#GrgS@8/ Ìaĉ`tWWWD/l%"D] @b(lH>1LL/r0OH*! &X6χ7%ܸqG^#r0Fؠbv'0 ^k(*p9x!CZQ%ĝ" i>=cW-X@H 'i쵡CGx@!6}.,pPz$8% W)|H>B2Z n_+PHI|q㑖QAdm@tr $Z.i|OU#Kt>Wp$x#Hqr~WNL;ti|D1ϳDP$S*6r.xBWX߼yÇDf<~'+1# 1QH1hRb3+`sns㨦OC,ĿϕZOjv^E"ٳg|%0|B!NM NM,-rnOSw޽rVYlO T2 prrrwwe$r a#֥KJUYY N<ŋ @"G9t&-B)ļB | G7R;S/:kD_$@<|%#s X:@./'DV > HEHTD`-8;\$!<N.m{q|Ha!)ى Qx ͒ZP,5.^^-ꢀpr,T>u9 6zBeKL8jeHkqt { H8M]fcxG‡tx@?qɠXka|@ܺf_bO4.|l >x_rOJ|B9F[T]BaVqE]~37s""X^@$?x~BZ"j7;( ~6)K`[L-"'RGקּ|ɕcؙ36/hь02䛈ҧtm6 q2Lo(XҌ"ںdE _HMlj[*-Nqi[\ {iV= I,(p՗bFsB ]HaQ)Č|qhqk+D;x/=QH—^@pPPjaM7ޢ(F0@D.!( |BN߉JRo w} |B^o2z>oDgk0 }NȲlAAA=ݻ>|믿VTw"AR aV*Z9@CBB0ՕsѩFR a6 YYY;v¬j;t aʔ)K. 83?)oV|||vv6W*Ӄ) ԰0uww![,D:={, @ @BaooϝL|gggn0:/۵kG6>rBZ>x`RRǓg!lٳgcbb_F#G֮]g "<99yΜ9HLL|aBrTRR2i$3fP8a"{X#0Yfeff%p 8fX/^|!fy޼yO<(ӧOSӲeJl΁?`Is qٝwעV!QS&.]QKђV<痽[B ltlIӸrwYuH".M`3.$O#H$/g7ntq[lyxŊH]/ /+HE`0u떑akk+z}@@۷uۀ~A޽qeܹSPP={zjeeebb"dBu=%%s휊GeeeFFFҽAg0tVVV7nptttvvH$~ A$D"ŗ/_0FY~}xx8̇E$길gggHRRٶm[faÆ}jkklmmrСC]]] o`mɒ%mڴK.//D'N}0 ÈD p9KRlll&O,xYӗq^c'K4 ۶m3 O#H1Lqqq;v8&oٲE[4L Vy @ٜ믿[4eل~b j‰ϥĿǎ"977ݻ`Nxnn{8 Pܻwo׮]B(,,wޞ={X$7@III;wr&&&ܹb3w<))iǎ i9]v544_zO>۹sg}}}UUU\\w~-Q$i4]VWW_pn^Ϟ=Kg6 wﮭ;u%$^gϞLl4sCCCll,n6ۧRZÇ钛JD>x ^jG]|YT޸qC'ŸT*t朜cǎTǏc($/͠ǏjkkG}zÃ\WWwqJ%zwߵ瓬bccKKK/>fR"J?~Eln:f̘,X"!;ΝdgsPPо}uUeY6((:t޽;Œ ڻwohhhpp0X"cJߐ={DDDp,qNvvӷo_"@?ų;::fee1 VgΜiccChooc43Z3wZ8^D"\./--h4ӧOCBd*JT644L6ȑL&.))1Lo񆃃T*h4yyyf7prr஖J:.;;e٩S+ɸI'947nPD{u E||@+Weҋ/ L^QQqYZJ̙3]Thӧd7gΜQ*;wB3˲ϟ///(]wʕ"<1!!@W^ nX^@b7m<v*jE _0X^Uho!@0z-\%T _K6}I/Ca4^6/%Ԓ/{ /D N@UUU"\ܿ?77W$!Jܥjwihh`YX޿????777==aXlD*N/))yZ$joj .)| Q㒇L B\___]]B_ډ "99aRaaj+++ZmEE|- h4j:55UV+J>DWC,0pDB6:Kc"/HTfee듒lllDzl@@@rr+RQQQUUSXXheeagg0 vAy CfffeeeEEΝ;/];88<|S$FDD8qƍݺuH$EEE:tppp8sLuuǏϟ?߽{k׮O7t:]iiiQQӧOoܸѮ];?%2[_X ,^&1%/...**)---,,w͞={D"JJ+T*+++sssDӧO#-- _YY ^ VVVo۶ѣG...JRR'OIҚwﺹedd+ǏfffvԩJ >>ݻ:.--ӧ&VVVwܱ3 iiiȖEHALGD(j˖-51cȑ#7o|-aϟȑN:ֺr\ v(rrr*..V(zT.͇H$JKK@m Wd"hѢER{ʲlũ6LN5k x}555 ^_WW䔑j ޽0 |Im tMBnݺ5''aӧ5ܹs7nd&00;x`.]jjj\]]A222ݓmϏa;wxyy%%%߈D#G!!!˗/dVVV?L&xxx80''d2kN,={W_ tvvoWUUچo ڴiSSSx )&&\ܿ ,),;55U$0 뛛o2BCC&eZ6++ Aս{={QN^t)cll,bhhz}~~~uuZm=|ʪ}YYY:us0D*|ĘmeeV\ .߿߱cǽ{e}}}#""eeef8^R EUUZ DDEEEEE1 SUUu6m|7 `RWW^4{ə3g|||233juuuuΝZmnnnXXL&K$[[`(D"i׮]jjꫯZ\\\=(-- LZ~H$߿D"3='!o}}drttl1;c:*++kӦMSgddxzzM&eoE ݤz,pVJ^Uz%s—^jvE?@,[ц!vWD-.q8I$~q"> endobj 330 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure4.png) >> endobj 331 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 332 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 331 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 20005>> stream xyxE8^3ܙ@$p+9uUuA]D||8˗={ MF7|M b]]c bjjoDQ+?h<<tGuVQQѓO>K/UUUuuu)))ruu=|MMVaZ_,[:?.˽***3f̐H$}}}oDZ WX-,,z}[[[~~O>Q555aAlllĂ_Wkk ޼y8>>_Dx5T4....\xΝaÆ DbpOoootTpv̘1o+pРwy'##ƍOjΝ~7 E.]rʎӧO?|… ΥKjjjƎ;|ބ5kWTT۷/++k̘1vZXX_򗴴欬۷o>|<55+**)))|իΝ|+W_~޽{&L2eJ[[Q^xJP(ZZZ~i^9sʕ+/<}+W^rS0 Rx`޼y,do޼ISdW^`^<>uuu===ATN6 UbX[[wޙ;wnBB#00:cƌ+WÑe˖M6mʕ?3<&HIFF` B3<GJKK9kkkj ѣFϺv-Jy5R)7fTTg}W_(ŋ֬Y#JSRR6mdeeuԩ^{WbŊ _Ν_gff>͓d{ꩧ{=H_SS#---p)66v˖-9993!ݻ.]*7l؀?___hH I`4YdD8UYYP( ;bss]P&*QVwttA%M4g}ϯ833s騶+V7o޼/eRRܹs A #){!Hd 8``e``a+;vSؼyk׊,--?Ç3fŊ~_pd2Yhh(7J$AAA666~&>>~Ϟ=۶mh4s3f q#FHLL<{̙3 <<<,--%[om۶#G/^sN//QF.YdG]b;::N2wߍ0aqUUUP;w5j$󳳳srr8.<<|ʔ)_~U`_P_P&6zh>֭ƢAjv޽}A|駃7nheefD%D^awwwo  iɒ%|mll`mFQ.A:Saoo@ "ARd%ZL޼yرc_}: _j޽{W-)QnOOBJ,E]T=ٙ#FP(ׯ^hj%v+hXc4\QL*"C&4w04Z555.]|[[[QZFb|)b1fcE.TnJ|*@dx2(j7dc5!**ƍ ߇T0 45j#&jj&#(4CU+5UQ)Zc{{{CBBlll ZffΝ;Hrl gTZ_$иĨϻO2C70L5662"(I$$H^߹smT**!勊,,,֮]wޞ!sZZZjjj09DjNHׯ[[[\~}dǎT㤂v{ U vvv]]]sˋ=3`;/^4(?WX!˩7nhiiY`hJe^^^^^^aaaCCիWϟ띝BCCCBBBCC---?Cn`7n;v믿C{K_W_}奥8::FEE655XZZ?~*//복 *J&޻w?$$dܸqǏ=z֭[CBB-Zh:;;*+++*****jkk333]]]9s愇S_b8۴iSee_|N"7 qN"ꫯYn.tb%̘SxCgN`#JbEHO2pd׮]]]]xi4gee*Fႂ &9r:44t֬Y3g TW\ioo={6Z`QwOBuJJa')F-1b2ױq2"bvQȒPdل&''߻w`05*""bԨQ[n9s%Kz7#!!! |[Ռ3/^B@`ttNknnOZ^iӦ*XӦM+,,T*֍p-T*ZɓIbҍ2^ g_/0RJ`-J !PsԺǺÙQTmmm؞5F-3ܹsg%ܹsÇK>&""b…(WWWf̘d8qEGG̙3  YKK˨ȷ~/o=zoڵ>>>򩧞6l`裏,,,^~e2#677_r _8Fbrb{ڡTԛܷ25]ͤ]$Byyccc;ZQ|EkDՖ޼y͛%%%nnn111sݵkSLL̳>+d 3r;vHAE GU]]sN*T*ݱc#ǰR(@)rL#hb&6yrTUDI QY OLHPtI^yJ'U f;WTT455ϟ6mڶmt:ڵk]vO?tٲepDS,1]%ٳ?Z-)bŝ|8oy£Z9f '|uI\ʏy…c/fA0R4** PRRnݺ .xxxU"C٭_^ !oRY`AAA\U{xxYZZJ"d x]í ~?ʲ0Td/3}:ÞefW !?9{}q7npww ڲe˭[ dVT&''9sf…2ĉUUUMMMOO_> zp۷og}rS"00pݺu^oooBB^<>|W5ӧ3 ͓ WFEEM<"hZ++nxK88F1PGH&T Q!H v!<8Uz'Ϟ=HIIR1clhT{nMMMllU>3ĄRbMeóg&''K?O|?񏮮/^8`0{Prss\+H6lؠ +G{{XTk.>'˗c&sd|9<(DRWW /=ztѢE؟a*H4*B= uPrP.!0[og c9`着_GQ^^p}͛I㜜klmmớ: boMOO7oޞ={BBB!#F0!L&O>=))_~뭷$D"ill{=5IjDPev|Ȧ&8ncc8~M2Jq3 11hH PB<#P_.8~|ŵpDT޻w/&&2`ƍ...V<^^^pE &@^IHHz믿饗㸸877W_}Uiii<F=.Ċ %C?'\x͛7 }SSS]]][[[oo/GD;v D M1i'l.nbIRq* 5*ehd2ѣ9 {%I||<8 Gŋo߾ R728ȑ#3gΔJىAAAXY!rss\-)d$P"cd9{)))݃[o_@c$ Q\I@Ʌ CakN YFĤTr>Ǐ)!e&#aܹ؟cJo8nŅ 3fԩSoܸacc3{`FQӟ$ ?B-L3ňʊ w5uT1XdF 5TL9!􊊊_~ޞ̖~#Glٲ?`( fggtMe\CD2mڴɓ''LⓋU*›IϽ{:88p7g771c,[,""^/b#3R,ʄA6ـ+kkիW'ȵNiӦ`hB~MHHbW111 ƁI P(EEE=:(l9RUU닮&LP(_>ydNf͚_]?oR)b_R̋bKm󅅅nnnʘI^rbh܆حguuuEEEgg'+ :rӧ=zrGyAm_ jdr F?}.++xf?J>)vI*y(97|hnG.!7mooOLLtvv>y$#?UYYh"3DOŐtww8q"&&<;"A|y7n_~…W^d[f&}F`2&V6ki:;;߿?,,lٲenq+ح|&ĬXͻ0 `޼y(!:^_^^w^LАP(Gvvvvvv?`q]]]?c||g}@*(b"BNyxR6lPYYyС04IH-]&Cf2%&iݸ>bl9请tr>3$g >aggߌ'DN\ZZzy湹hjugg'RՅ;?IFcKKKVVVccի}__@>===ɾ Nh4mmmmmm---jZVxzz޿3mB_.ɔJeDD>k&+q'Oܺu5kvލEiEM>,[hJH%!qRT&Fwliiqٳy;1e ٳ׮]ۺu2z'++AAAV 'J-,,,,,#$NNN^^^ӧOOOOtuu]f &'//ڵk7o8n3f̘4i\TQ[[[]] ammm4+++ CkkZh4r+::zҥɰ`S8j  5~ ^o4R}}}{{{Q/a ؜ \lvmiiO]… /Ud#XjP&"r&K-d* 0|ddsӃC0B+Ü9sz=@T!D{ u:Mpp0|eܹnnnj'u3`4]v9R]?vym޼59288811oWyYjuv???4BQZZ`mm-˭zzz{{{]]]<==JF222xK.wPIw5۶mr@;YuԔ}ÜFu6؂Rq0jT=quO<Uon?l"&&Vy  ;wnPP7[=//_c"ѡR?$&&Bf̘§Նg7ˈ#ÇuuuG}}}1OOOWWWXDcǎ7xQxXtǏ_VV+SN(@xig ``y1۩Q3BaՉ s Փ: RDՙ`Rv ǥJ1$$$$$dڴi/4Fkaa[o>}r'liiٱc뽼;vɌM5G?4iƍ &L;1f#dDkhhP(NNNs28 Em޼Y*7i8͓Tn$˃"T q bIyW"899}waaaRKfìY6nhggd|lA"1zzz.\?Ο?Zeee%%%0uV&mذ!))7߄30 @(-- I04th4c0zzzryMM |ڕ!iӦ nDF: !*r'2Tel3;!d$J$8|@CCv6 9r^?~~Ietׯ_?rHQQQBB͛###uG34wss[f |©@yyyiiimm%0bĈ?|P޻woܹ7oބ/-///..vvvMKK3RGGGW?]"<lI`)D9D"sss>ccc EGG_VDN>ekk7?><<#D"W1dPTTt_~eԩSa O\\\K#``8suu}!Tl0777::ZT/z {JS*p0))СC|I0rAwfl+3F "֊%GN޽{:$$T__?bĈ|%==}֭&M"NRj rJhhFٳgσR#&L0nܸ#G!ìP( FǏ={_O+QB;s SFi f#)SL@%[Ԓ 9S*---ϟ֭CovJ$ꉙ+B(c^S?$vF0򊌌,,,3 eəÆ .tssOq $...##CP/8R(ɥF1,, nԨQ'N3gΜ8qϟ9s ۯ5z ^&cH/9 :uBv  000TꆍW:ǜ B5#V9m ?%#mll=Pl&Ãz^Frtt/rrr>\RR 6a„;w޺ugٻwoppfy!Z " `z05e1r>R RFL1JꃒP4ucU:.Fh^z(D??Q!!!7n܀&ㇹF'@.?~ܸq<''端ڱcǰavVFMPAK2ߩb1GQ9cIqSBYVy?9R*-=b ~,ũja>69;;1cDGGϜ9_~eO?>-TeH̭lB1B9@ YI%%L&rww.vɃ(5wy!w]Շ "N3"#(gxVTO$V(S* Wtv*Y77SNYZZ*7| ejЌ k@&C;U:RGJ(@^"\t ^xd6P+U(j@Ѩ`mm oƘ6EM,IHFBxvQa„ gMcQeW,J4=H@irPhYJ0Ȗ&$ц w̙3ȑ#cƌBqDҤ#Ft:E &-$Kd;h ҉1>t萫kss3#d)恘$ATFP[D!zFlmm` '3`ܸq$?CC1L$i۠l7{0zxjFcggo(!ܘ`3+i  OӋ9%ҫh)HG@a3P\.߳g)C`!QEl$ЕRj2y)K &_2&ԆKR pEn8H?O֣a z,S+D L,@ؒߤBI&D`擦"`f-$lSR7/TH Z;vMS q ։mǥw{]H^gIBR+Bm9@9S0Hq(C14* Uy1+C[!Θ4fhԈ)&P^7@f`# fJZu< lܯj$\IdCI8%\&,ɧ9-- >09)ƁD,gԶImdfDP0 MIm/_,;;`PE6fE(~ xY``sb~'[,%'I*m)kjju֝;w좚#d2v,T( 4aBʋ1=f)7pfDp9!v}뫪"]L=f` ]$ B"N[+["G8q"|ԢE===T"a(c2 qbz[[!UEqR" $H${{ qF 'P6C13 @lvttX  P'x*aHC%HA߶F*ʑ#GB^xBb9 ~׸eHA3%; ŋc`n& ](. $|&?Q!'d2CЭb6-73@t8@L1oAtvȚ7%"3B̮R`et !hWܶcq!uM95-!y<H*mgmЋ\DPXQiGP|hLJVqE-Nqn@6ֹ4%_BU D5 nFΠҊ1*K0D!)& c )naPHBG @`cP-'Ѱet&3"#*K=nSE`&01/j8R:ϟ]TyndԨ> [C-Uy1"ڢdYr ?nE ۰q,lF&JB&ZhԆ` -(C !8hm\ؒQ#S L%gT8AT :ر OZ`-I(RIlRg(ժYa2*l*3i2V!l&hB9" e@u] * 3V1Q QXR"(Ft yA *uUA$h Qd+S& l>)1 o+!&"@6AC"xkB*.\)v ]i6°fBΒK!#FS j(D5DL<`o<}T Fu# 砨L:Ƚ*1-6()cJAܶ$f߂96Cy0ؠ𝞁/A5ݴǻ GY?NR__ѣGcS"gRῒ U}z`i|e_F8U.=" j| _BĐerxsH)C.Y$55u$11СC  Zjڴi=T}}}QQQWWBVקj~KcwпF qpp())# .uppXx1Cܞ~iNrry87n^v-իWf\rbधWVV //`TVV~7۷og}w[ne8p`ڴi! ۷o=3ǝ9sf˖- BRZV-))6V|(?Ν; 3g8pΝ;TArC|{U555H0 ŗ/_>uOKWT={CUUŋ<($O>!?<88 z}bbbQQѸq$6E<ܨ( !4!&)))ƍc 7|k4mllK{{{x7^&NF KKKGGǴ*hJ...---Æ #q8SZÇ^^^T0iҤ:,Jjhhprr1birPTAAAF"dff7.55IGz(~!_D봴41 Q===ǎ+,,5 ٚSAP޽wa }7 z}{{}%%%z+ CGGh4Bax>z`0PqڒP^O ֶo>Ңjlnnvڑ#GJ%#|R3?@Np]// X[[ۃ_ i?]\\M6zh*D$-;;{!!!٪ݹsg9r$[C*y<9NB?>55]]]NNNZΝ}v{{?Vp¹sܹ#!.ct:_]]M5D_x>>>0 ҥKΝ+))`4322Ο?&oooxꩧ^{5Ta2LJ$L&JϞ=xblz5v}Lhmtqpp8uI؈`;::yZ1رcuuuOFB EC8uRΝI3?սth׿vmN7aggS ޽{233 ĉrQF~?I&Ϛ5 ˟}4t111vvvbmmm%&&6..}ǃzСckk qP>tIHHѣGN:mwppHHH 3wޕH$/_NdD7VQy&.\pvvwoֆjSM]VrwwX!=IIIuuu-))ydB___JJ[qq /ovzX՞9sF*:;;M%%`0)D5Luj%,J_}Uf HR{{{//^x߆aBϟO0ܡ:;;Sd0"""Lot8.88Xь?>** *;u@t4lذ'|R.2aaa3cǎŤ222?Wɔ|N۷s̱ԕ{g0ZXb Cz)\q=<<ȭ Մ~ڴi'N  N<9~0ZD$3gBBBƎ[__?f̘1cyWrFCXtiII %~UVVzzzZYY yrѢEMMM| ,Lz̙3Gt:F6iqqqRzM̙33 !0OIcbbBBB[&Q,V3w܀ W lrM:ttt|wgmgg}ƍ@ln[c D ? ٧[ظHRq"#Fj#QYr0*a|H !Hء-ӤQh7' DLmqG P̋gT:-T#sQ y$C1IBc8ȯ_Pdbd(!&5y&!` uq 0(իEI$"Id 7Hk#9_~C#8,_-R[ѯeojjj<8>xUT*JT2XtttC tn8`0ܽ{6??㸚'Aףvuttdgg?|H]@TƜFu/Q4pclmm7.C䮮.Nq\CCCvvvnn.s~{{{__dCooJ"7oh4';;8z?*q.ꦦ>`twwcGR#w ]#6m[{V6/Էkkk Saa!2l߾}<0a|Kcccee{322jkk;;; &JyA------~+W[ Eee%<%%eĉ)))~~~cǎqFkkkXX\.OOOoiiqss+((?yq㲳}}}o޼P(oݺ9  X60p0!qTZlkk+//U*]AFG5jmVVF)))qpp(..6 A[n;aaa^#X]] veee{xxH$ӧOO:~rrrʂ߿:22~...̌~{NN^ijjh4* ޶>|p,dx {G%e*{?ؿii)g>}zFFƾ}_'NX]]cmmmmm$,,,T*UsssGGG[[T* tˬe2`ٲe!!!RRxÔD"quutR||ɓ'}||Fc``T*겱1 555>>>ӧO/))섍VBlM:PB\!I)DZ/^p0nܸw}W&{2L&T*ӦMjժ;wz@MMȑ#KJJjCxxx___```AAANjggw۲e!T*urrtAAAEEEqqqݩ򊇇Ggggjj9l[[[lmmcǎU:~xx/| DxOnQhA?s}WyYfErwwW*naÆuuu)fX_un^x>wqرc/\骫ju[[N Q(666α< 1 NNN&::g͚vyF[3rtҥKjunn#GYWWn/// L]*CBB|}}%/pvvkXZZbƔw8qm-888--㸪*Zhmll`GQSS~](E.`$$$\|9""{Z s|p08|H8Fov2!j{-D"πzzzb&"@vz`߱V\\lii9uTT$ ᐟU*-^g*&ޤB(>OB3Tg،IPМmH`9Lz \Y[ c5&1cP|AߨXMTMfl1UٻYzB"{" $OK7cJԠ $lMnٟb I%d :.^ endstream endobj 333 0 obj << /Type /Annot /Subtype /Link /A 334 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 28.4528 260.2500 197.9528 ] >> endobj 334 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure5.png) >> endobj 335 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 336 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 335 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 41263>> stream xw\?>z4!"؈%h4[,c4hhQ4*(" (H* swݳzy<<>gv8q "`xȁTP)&&A$ d~&X4&8,)QQuZ ".}TTvuuADbV[[[^^TTT~7^vfmmm-aRdeeeuuuPgϚ` N8!/\p}΍7‡+WdggGDDǎST\z/111&&4..cΝkuuL&njj*--Qmmm*z2憆7n|$|W"h۶mǏbqCCaRYYYQQQgggkk+4^$AW777K$,TaX,.**ڰaL|rXT666fgg+ LVUU^__P(Riuuu[[aXgggii)Drfav`յi& `ɁC| sܼ\T{xxx?sذa<=|P"ttt؜;wӣG^r~xxxpp^[[{-PW_|CCëWvww:uJP:tHKK;rHKK=<<>SJ驣3uT>ogggkk{O?444tĉeeeмׯǏ뭷v5a„ѕuuul f̘T*#""N< ‹/zzzܹ˞}D"x񢏏ObbbZZZ|||eee``Rwɓ+++ry[[˗-,,rrrlmmmmmR-yB%|SqqqKK˄ >}JKK9RK,--kjjpJϟё[WWwT@QQLP(233߿eff{YfY[[>|3!!811,44СC&MڳgϿ|rPPr͛77nSRRf̘i&++7oz{{:u ðð77gd_UT&&&~_f ;w… 555Ϟ=kggWSScbbRVV[Ž_~ӧeee~~~aaa&M"gXɝ,%JR鵵hX[[[[[Rm۶gϞQF988m޼y֬Y׮]`dd~zboo޼lڵkYYYuuuݳܼy3:::g͚E?!!)<<޽{*ߟ pŊ۶m333[vrqqqqq9tʕ[n޽?iF5{l̙3̙󎎎Jcddm۶s疗[B?0455sKKKmmmSS СCAAAo֡C~嗳gLX222 E__ 477_?Nbf\WWf͛wÛ7o̙C <<<֯_f͚5k_v!//e˖}eeecǎz~> jkk͇ F=zڵk?裥K>{ رcɒ%+VzG222n߾qƑ#GB6///'''gg˗=ȨXx-[ꈐ~{֭555?;w͛]]]kkkuuuy<^__L&ѣW~ ""⧟~R*<t8kxCT:i$49mڴ[nwvv%%%Æ +((x7[ZZ Ecc#FyDS^>Oncǎ駟(ux={̙C6#y/aNNN˖-~Ç_0h'ɓG #RH155nhh:::Ӄᳱ͛e˖ӧGGGO>6U*)@{{{{{ಲ8w܈2É,4FFFz+++O0!%%:qds1bDjjj}}=& ύjjj/_P(oNʇ;a„'Oܹ355իJ\ 9| OOO孭0DWWB )3&MtǏG 8 :@@7=%3 g͚аo߾;w={ 2d͚5;w477O<<<:::BqLJ r۷[[[Cfmll͛wݻwwuu]r,11qnnnWWӧO_paٲe<{{{ݝɓYfFDDlڴRtӦM7n,--aO8d}}}ǧMsN ChiiA8C Ԅ!zzz6lrСCq8[[[΄nڴ)))iŇR(>ӦMCze겴D jhhoڴ zaÆEEEccc?##9**jҥЁC ?|pxx+B)""nǎ˖-KNN311ٸqcMMMUUĉ! rJ$--9;;'AAAVVVv  E~~>7-,, baaaTB啒sN>:;;wttXXX@3z'|rС'n߾=00J&YXXPq*Fg괁dJLLܾ};(ʇM)GSY -@v?Í7~'OK.D"Z{GK0x<8?"E S$&-@۶mKII?; y뭷4PP̛7j'L*(h": ͋Aw RZ[[?3naa1n8'y{{;::R %_[/w u*HOO7 hjj0񣶱M>ÇsT?Hğۃe7bJ Z؉_"`I(DRRuꔯi+**zzz<<<˟?T*wQ[[+ &L뫧-"@M;j mc t%pQ0Dd٫e*bb#J $kGd~5B(QhҢh@YYY9U*UVVVLL̽{gϞ} GGGRW_-^(ʊGikkM0aܸq555˖- jR,La@4{/x _ٵpB1E A=,i `%y_5`{{'OƏ/ݻoرА6j(zj :;;322=ztԩ6??_ĉs555522b;==($$}̝;/΅)yozɓ'oF,O2eݺucƌ!NH|>l̙3gq\P(!!!%%E$b' -,,,--yyy_~ezzzJJ <|||&O<$OPzqooo@@-,,cf q7w)225qDcccr,UUUFFFBG*3D0 [t)H$R(>T[455Nk.GG˗/kkka*J,Ꚙ?~ҤI#Fe*3T )A%%%ǎnܸ9zLݻ+KCL~O/Ř3gdggl㸎T*2eӧO{~mff&ඞR"""V^Maq4>>>!!m„ 3gljj:r\.߶m$(Zfff.f92##(x@ ͉ ѿL&{.>>ZZZWWk a }ĉB011deeI$T?WHH9 .8fZtTkh4Vm~DW )..O0!66VSS-XB###333u'O 2$$$ĖF@XXؒ%KYrb&R۪Λ7ʕ+SN533xw?}4///!!y8lذ3g]xqd"mmmOOO9͛7̙3o&L,J\]]KJJF xxxP*!C0d~49SôI a;n j ,x+``CCüy󴴴*++===!/Q!i=FV?Ϛ5ҥK---iiiJrʔ)+V8zNXkk7xC-dH6i$ڶlOKKKJJ4/dz6mqb\.?z@ ҊYh.j-!p@%OQ o[pܻw=##q8:9J.. TA{♩(Ȳb!ç'B(+ oܸ{4r\T*MMM> O$Dhhhxī)#'SM @KKsΑٸ7hqmm%K޽ѣ&MڰaJPB[[{СwXҚAG=㸻{GG\Z R) D 0̃ZLKj]LT@J<,D>ej8əd)vuu}!<!0,444 ŋO8ex;@BQ ?(\^QQC@Bwڵ^^^_uFFƌ3v=u^1xOoGGEGbOhp.Li!miUe)D٩T*Ҧe;v?:JyyyfffxvLSXXh``$ѡT*ՖOs̙3gBcҤI6lV.vh>NG2LWW@KŦQ5zQdd2@V&GSkjj233޽KB</!!^HI8Ϗbرc0ٳgϞ={IOO5k?S @ZP3ׯg6$`9x!L_(@2_ǎ@P CCCQHKKɉ|&:#?~޽>H[[I|upp l߾}ĵ% / b'0 68q"22 #jTD/^=MMMǏ/))E*\Aj(vttJE2J._+w+ׯ_W(,i)%㵶cuWu m(puNG.I$FE"Qii,EZ=@ P*Օ555$333 p?qĹs|}}'LL‰g"˸X58i ̟?;rȹs8hѢ5kXZZҖSN͛7t 4'$$7bĈ x3A6YD P%4LNȁ(ܡ6FP(-4 疖&LXhTGzxxϝ;O* B'''xQEE_=jԨ B}:''5 ERʈKi픨*0͛/_ѹr__ߣGd2ˉ=&qvv5jԌ3LMM'X|ҥ zzznٲeȑJL՛.#F0MMMgϞݰaQFF… 2)UR(.ޒCrTɯe%2 A .QS'NheeC=zo_7n\VVָqp!Q00--ðkΜ9xjȐ!SN]j<(((00ַϟGDDܹSSSs̘1bXSSsϞ=WvuuH$}}}===3ZRR2fDb 777---mmmmmmMMM]]] ===@ܹP(6l؀g񏁁 ֭[|AH~P\80CKNN퍋;+**||| ύ7<8m4b*L! z$SI7FbD+$ʆ d={rV\PP,--$I~~~UU QTB[IhKVVV~R? ~뭷ZRZ[j> D'NZXX|7...p _OO@OOVTܼp¡C»e2\.J CRTQFiii566Һ6/Ȝ|>?88xէN:tмy֮]kmm]RR% )]ѿ_q:7nܨ B?~,cbbˉ@~¿jjj?~ٳ[nW_ݽ{^1ӧ γg.ZwީT߼y|g egg/]tӦM~~~IJQ]]sى'.^8///**޽{2:;;9;;ÐqVk $쩘:WMz$T*mii!&0`ƃ~]kڵp6LT^y.10jժU}}}7nI,002rϙ3G*]6/// !H i:=eToPEEŵk׺)Y pb203Ԧz)FP\Ryd999,lBjkk;ONRPPp֭7jii(8Cb:88Q444)-@J8 UTTX,^lYWWoYCNJ?wQ8?gu'^탂Mprrrљ9s&|%'N ISSs۶m3xѢE/]4{ӧOԅ L6mС ¼lbBPBiQf0v WPB.&bj$Pd%9@S.iaݻw$W;q0X_^z՗/_vwwG bTg/NlJ577(ai--*!gKIIImmmGGRfLCླྀ#-䴀8'6oW5%L0Ph@M((=yyyX)-d}VVʕ+CBB$) @&YYYy r>55F;AOO?#L"7&&ٳ~B b~`ff& M033kll"R(xZC;h2ɧPlf1b,rg٢mk)(ZLMMMܹ# *Ǣ@DyU*[nݰaB=nџ֙䴖_#WG͝;əL9hnn~U\vtt(CCCOO7|#!CKV.W*}aRloo ?٩Vbo=hnnbhhhddqH<ƍϟi G1 􌈈 .422600:::?c~+*TJ% yX===AOO4~>oiӦM=ta@0?~ w XXXXMMM H-}H;|' $$9VQ`jدHHUt;@&_Q;v 2QE\|^@JfEiy(*D7o @>Ci'0a„?XWWhB~ $bb K&2 .|*Ck YPJV3UN!hm۶m̙D%0k"H)TKvJ,dDz|vvvvww'/4fӧ듷q\av?$q11%%%-"bKȥp1Pڵ ^$‘::bĈ\b%^(OOF6+W֭[/{ӺKRՒWLLLmm-i $@v/( X @wwwyyyll,GKLVQT*JVt hZOˉG!^,X ) id!|wL&ɲc}YhI, E]^U4W BJUVV6t~'_)FGuuuRRDM'˙qtۯ +ד.8??޽{D emlkJ0JuƍtɎRL&06xeY`7һ%*55uLzi!WTTܻwc_b!vs]8G(C )--eee2J[N<_ڶmAbv\wׯ7n\HHȆ s%%%UTT RvvG}TQQwߡ]4dRrA;gΜ WJ+&GJJNNϷl幹cƌ8^XXH|CR269MřR4/bu^^Y__@./2yyy7o$wf y…20#DH$'NߴiSddH$Zp޽{![ggguuuMMMuu5\$d]]]C?H@P,el$L!2YZQ) uqq1ϯ9T*)K?NE?HL&EpZL6Ms[xv6H4d MMM2%;;{̘10}HHƭ[X[IOE >%\o-[ eMKK{ȑ# fSSS#GBGrrqmmmܹ3|y-\Ϗ|:ܹsҥK EZ꠵׮]+**ruu/f uGAJDڙ3gΚ5 qX\SSTTT4ydwߕY@ DDRui~u#[EAAimj vZ h9(v;__YfO2ngȢȁMMMEEE:-[<<?gΜ[[)S0 (ȯܧH;1A(؝ZBA>Z,8 lnStƍ?XSS~prr&w>(W_}5zhaXllbccF_(^0,99ٳeeeK."8?04iҙ3g6o|…C\vmǎ#G\`iYRU1vww;v 4zhx3=&b{Jox{{}ebzFPt,(ru :PifffvvvWnkk6l߸qF U*U|||vvD"MJJ\]]/]D>qOOOٳoܸ1f̘m۶gٳg/_~̙SN:ð'O\vm߾}...'O177?gaaaAAAqqqSSעE<==---1 ;{lddܹswܙѣGO2 A4=ϟ?d߃aիWG}СP.U%˅e/R[rl?fߺuTˉaXDDDqq_~aXYYٓ'O222rrr0 wwwmiiصk䢢Ǐ'&&\r…oZR*bG@yy{QfH?ӧy<Ν;E"7o޸qѣ)d2YfffRRRJJX,rرk#?&'''''j˖-&&&111 iٲe{}wnM܉>!L"&O /T*-..|kjj,,,V\+++}}}===>}SZ핕C c)T*qQQQoON[ҥKÆ 2d"?TOO` 7]]]3g+)@jjj(\J577',D)fffׯ_wbMpc!bbbbBCCqӳtqqT` Μ9=4&L(,,g1|U*yDhBB[m4RۭYOx*RvH/ }MII INN"<"A:u*vrR!!!V***'dS600rʕ+by<)7N(444%%78vؐ!C/\c %|@ !ڌg$$$KRڂBvj6{]0 miM"ІsH+M;9@&'''CCÒ'p͛i"jhhQE3x޽d%VŴiӖ.]JAkyx<ޅ  k lll F 9s?{o % qc>gΜ'3XZK0rHB3y䚚hѢ;wP 5ZC- 2Ҧa`R;;b{w (kiid2pZՋ/>vdzw!gΜD}YTTԎ;0 ޺ukHHHcc3g"##|Mb0 3gξ}XnKLJ==DFF ~Kҵw\wJ7666}Ņ6  ',#u& #Glݺ766/lmm׭[/Ku58!88;v2ox=Mp1HaCsiSq$ 666<ҥKִ6N`VH$V(|Xo8nccn:&QU$R-dA3AVkKZSPӪd ,,PMdT*KL $+**hdO6-CqqkWWj9# š8`F8^XZ  +S.sHR'Y\vR`m_+? QV3((YZn?|p͚5\6+h &L{I? -   ,mreIr$PtQbXx W&J8 )H:&I& ˗/X$+.Z/xѥ \O |y )( 9 ǘ+$KKvB  MO?D ᘊIE+KGGEt#\ C Zi{-T;"“,{T`&!dQ  vW&72% |fv,f:p\q 9(e :BK{*B]YYYppp^^ĉqqq555qqqĭ+"cc㐐 s\\+7o7Rsq6 _sssꤤ$BF"4yyy9qY&ʏaXIIIzz:0&0֭[ZjhhHHH b?H+',QQQE"QttZtdQ!;77͎Z QQQQb#˗/7xz~Z{n߾܌5 ZZ^^Ύe`_+S`,u .NV̡p/v 0^R +hzhyǴIKd|%Z!\$P%ZDpP=Z ^HU-\BZUY}0LA ހ 'j@JMMmjj"3P@OKKkiihgZZZOOG'OT*(}}},=JKK#!Hh3W,HD"Ǵ ɣR۷9 sG,J233LRRRR8ڀa؉'Gyjb)Yd p iѣGRСCզhnn B aaavvv,z]SSS̽/^U(\߿_^^8dvNHPRR xb^^n*(//?DB)%%ԴёLKKD)))>>> ϙ(,,Ã|-EEE>x`ᔳPzYmmmRR/B>|RTTg`JJH$qss$oAYȤI={e!===ggÇX6113fLJJʨQԚP(?R9 HuuuQQO:;;755蘘3x<''N.f"?~?OyAC x񢾾;T*RkIeeX,GggH$eg655 7n\GGP(L1[(&$$;"蘙9evjƺ2*… 8WVV.4),, ð^v88ye7ٳ{ii-,, 񂂂6f;w0x6B%D"`㖖f%HK9998h8~]ꊊ233U*UrrZ҇b.(*++ S-mm-ӧeΝ;8766#H޽Rz{,ʀxSSS\\\vvӉFoii͝>}5eee3ݺu+'''88X(_f2b~O/vtthkkKNHH(((ʂ/?RABRF1uTuegggeeԌ=ČxNNΓ'OOX奧?|ҤI RSS+**LB\Çϟ?6mțŒ䒒Yf{Dg4>=5 OIIɥKd2ٮ]ݻ"P^^ٹsj'+**.]Դ{nLݗ_~ ^:SP744\t/k'd斖;vk'3K.~gٴ BD"џ}<&KŔYh &&&::: ͭ ׯ3f nܸ;j(2ׯ1߿-n߾}5OOO8D KsNddgHHHQQ;aÆ͞=ӧ### o!Z=!x@7dzEn߾gllaq">$&&(Jsse˖QHҾjW`eh$a">0 _zr"Ceׯ0H|8$Q;}2-?%l!L*Tˠ0,$p :0NbZT KGC R)C-_PX" t"Gd_Yɢ,%ݖ ZM$ %r Y(Of@2xcccbS|ZZիW555nݺmddnggGR U*f2j={DP(PHFFF~~~[[ 99>))k蘔/*999"^(fff_S>L>ddRǔ#,AJT{{{qq1RSSmll\]WWw----Ǐ{%溺:cccmmmNYx8r@0 .**~L&kooҺ}J*))T $%% BR xXа`f!%"RVG-X'!{)++X7UڵkӧO/..vttD:::uuu===r[XXd7o P$ njΒ<396L9m۶I$@CCCccq͛577o-ZTPPlx|>_PX[[+J>/4EPLuV{{{mTTR@"333-ZT__?mmmO:::nnnyyyÇDb\T*kcc@ hnn0L.WWWx)WZ!h }[#ЁJ200H$WWWWT>yn+ՅrnƇ;iG&!MMMzy === USSڵvvvphAARvuud2b' ‘RmmmɻdR311d ӓPfC[BQ__ Z*67}w\TG]veU:"b`}wԠ-ϓĘ1&1k5vaYX?03wR4<393e` vU |Rl4AY[[r^zW8Rj6b?_HRg#dw03rbM 6^G,G'-8>|e. ^%2 x҃F̥ucU#MB.LvW|Ґx!`q ~$#! Qr3 qqL. >NHqէO9;;;::syrrrkkkR)x[]]'J]\\D"QCCÝ;w=$>xf0<==E"V53"*--ljSSS\h|rZZZCCB|}}% xËa\r<0Ʀqp8|kekkkwekkkϜ9@PvvD"jNm èj!N3xSF٦MD,HP8998q"555//M6r<66655533ѱ^ XK0L@@kXXXjj*H߻wo.]ߏ?~\:99gQ>Gx+z~ٲebx֭O}(J#G@욤8Т%gv 7oޜ]VVzk׮999%''oe]8D۷hH$oq)RQYYy]ew'''++KL&W.畕t] Ilŋfsuu^^21cϹs|M{0`@`` 0f/\dӧ2gLL̕+WΜ9#>%KGGG'$$k… g^tI./_|%%%˖-x񢽽W_}e0>S???\խ[˗:twٷo_5==]Vڮ\r۶m'O.++{6ls,˾۶֭AI̜:u}Fh=zT[[jժÇϚ5k^^^ bݺu3gҥK 7lؐeEpW_}~ʕ]tի׀jjjgϞB [KRG*;/00pذaŋzw;v찶~kN7vXy3&!!aǏԩSCCӧO?Cooo`0L4ҥK L0͛ G 6lXhh(˲~!0aaaÆ +))ٹsBG 4rHǬӳgbbb.\q7N0رc C_jJJKKx{I&}aaa3f_8dȐ8p@.k;rHΝ;fmm=|q}:.//رc 9r$::Z$yyy]t 0l0tݪ 2SHuk4'd+>|ӽmڴ9rdquutz~6mXf (۷ɓ'E"QLL̠A駟2 cggWQQqҥq%'';;;K;w,;}t^4iƍ={?1 h _,\pǎJDDį{k?좢>30t_W^=zubb"$l6/_|wᶍZbѣ8O?͜9S2 SYY}A?Ν;/\`0 fΜG1 DfĊW>M:Օ/.\6lÇWZ{t3gO?x𠗗W߾}c0F5ad̘1cٲeϟ߳gO```>}vڥ룢6o|ӧGGG0 ӡCalܹܿsw1 yyy}*jɒ%sMHHtbxڴis7iii}y&>}#bbbLy)٬j ȑ#7mN)Gϟ?vZ.^yyy... $%%͝;w2ݻs޽{ǎ)b R^6'FC ԀqS\<==쬬\G >/IiiiGYlT*=zӧu[s +t:#ץKbL&aǏߣGD"Ja RD")N z}Qdd b<$|5_G^TPPjat:]6mLb2?I,h4٬<<<^ 8N~aÆo X@~[wuu-**t"֭[Ńz*w  Dz͝0Lddmێ; c#<6,U Nx 43 :411qݺuw%ɓǎ˲… q!\9ĉfyɒ%pzׯ_'Θ1c}ѣfŋh4WNOO&ݻwae˖mܸ-[rOh"{{{n77sYuvv|rAA|7wQQQ .,(RW_}y.]P!x(eH/^r>`nȑ .ܺu+0?̙3}}}W\ɓ0f<,Dӿ{{nݺ۱yxx\reҤIyQQQii+WFW( o߾a-Fqݺuǎe2ٙ3gWXqKwIKKKII===ϟ?P(ꈑBO>|IBB³g_CCxc'Nrss䠘[@W\Yd z}~&`0̙3{^^o0F#kZ8ck& V >|ȇp\*XF1xɤh@ pF:TSSsЬ 0۷1o3/Æ!+WRF#0,8f 8(n /*g,_sNNdjpt:h Y /Ϟ=yfMMͧ~ '''O`_`0jjj~7J$|#m!T V++}q:H<]#D"8`0"<la8_XfNV\2{l肂;]vgM"N&чݺuPg$L.|\@ylHP!'X ߅ N:q:w\__/2&更dnnkΝr_MziMZV2"ў-G%t Ѣid)1~cсͰT &O5K'aZ&S÷F$<;9 @I?Us\\qhE e.'?TH|'o .|`\HJeMJ\I㳕O Mx;2(fX5VktuSY࠿0_h6_KJ/37t!y qfhEht-5 BZT SQJTSEh^v zBN'JȀ'VlȌ*H![` 'zѕ!D]3DHVK3Q<BDfdNq0#4 KP q]O-I@CHÇרDWG(D"Qx+Ϗ%DhqH֊V5{ (Ps^HL-mj'H4N8q۷mmmG e w9~!feh'ǺYܾ};//ӳgϞȰoغ u-qEe}۶m=<< ̲o߾kʨ/"NX 7sjz`Kye_/y^KPG[$zR.^X__sEnl{B p8OE@b%_:g(` L㱐l TSS߻wop|Ox,~K@-$BsBی1\k׎x7,b[%nIEGG\pK@8x |- My $Up(qpGѧFK%5i a\-9"#Uޒϙʗ e%U_zLJ!_ @`37YXi袻-Y yO:e0*ePQ < '\E kƛj! GoS W t,D8 ݶ Lm۶m۶dZG셅[C7dj9~ ==+0f|}5q|hm[թS^zQv7. teee...-Y W=`6LQa4'L{K.ݼy3gRRR***F#IIIXR>W0PA!jG="V~<֭0òl\\?52 05 b/0ѕ5΃$XR6`D0@D% .]ossB5@DR`F.rooHHcǎ\Dh4R߿*GGvuرcǎNJ2..uȐ!3 s) <pFٶm[XXxW '9.K8ڵkrrR իWǏ1cxyyA60{a<##|>y[4Xp!8?p!efIIIa._RRRگ_?!*8111 BVoذ!00VT2ޞKa//_bl6T촴˗/o޼T&tԩv޼yGv4MCCC]]w YtZ~wwf( pSxxѫ Q  Cyy9p_@@8o O0޽{cbbƏoq fÇ E fΏ $kyXGw1YYY;vdW^ҥKjjjEE'0'ONHHXr%e7 /|{ aHb1wܚE >L򺻻'd Àrrr=<<&N F`dkkk=}taa{Ǝ\QQymp̛ZnhhH$ڲe^_rرc}||\]]yjz=zǹ21 3bĈ²2^RRRH,|8=%RKmS3>WEN&cʔ)=zWߧOiO (JǏ_Y[[GFF0 22t;vt֍;%deeеk׮]ƍ3bXqvvx"2@emm zwygΜ9;vܰawFdtEEEEEEEEE0&Lܹs۷o cC'J+//@q"WI _<8%fDn/B=_A#*I\^Rx-Go||{g b-JAAAaG>x;w >};::rE:y]va pgELLp, ܇ 899͞= Ds2mmm}||zͥ߿ȑ#JrΜ92,33رc999eeeRTdeM|X? qX5`!X7;w0]b)szJ|6 ƒg9Q#Q Qg\;Tqf`mmM C w:uT^^ e]]X(RGڶm;k,ggg O>d„ QQQĦ[SS3mڴ#F,\/|(J#Luuu .ӧϜ9steZmQQѳg233333sssCCC׭[g6ٳg%D}%%%}WK񦚻FL$!pkD%h!`B%#HD8 .}߾}>1bU7ݜ9s~嗞={nذ~e2ꪪꔔpprllٳgkjjb< vww|ruu58_;˲hApD˻vz^{V#FX~=qӧ4+Hfn׮qC~Ddcc@Կzݕ|ي3f X)DpX.#^4U ˲&ݻwG) e2?Htss׿viP6 C]]]џӧOVTT؀-ƍggg'zX,6VVVFwmkk wddŋe2xOׯVݱcǴilll#"/_vss:t(}e꫟~k # N߭[Ν;[lD-6GK`4>|$$y1ZjFqqnj#$cctttϹAH$߿jj* յk׮~T*+++ׯ_߯_?F0Ç:3RjjjT*UuuJ wppعsÇ!9VVVֶO>&iҥǏjӦM6m C~ ;ھ}]FDDK8Sk˲}vvvAt)55lm7RmR[("FLs>x,i"l0@ Q'.}ce|6˲ҥKGC sȯL&suue?>|xHHHll,2 2 RYYYeeL&.# 8uĈݻ7l 5c7a ǎ+JJR$H';vh4IIIRffVСCXXؔ)S$ɚ5k\]]###Mv֭-[}۷/ޠh4LM <Z q@L #9!n2  "e++@x˲l߾}o>h \ ׯ6… ӦMK?q>#+@D>ӸBHf-9daRRR&O  ڵkQQQ1PRX5kVbb"WE"x=H弶f͚+Vd2[=QF9::r6v1c0 c4T kk.]XtAWX^4iFJ,|L8Q$]p,为ڴi.^`0͛7`0444׃7CCC;uzի>}ڹs!C 2T qK!a3 s…^{]իVVV7nܰa'|'i)M&Snnn.](!Z Ҕ"^" "F<#7`9!M.kkF.T*0' V8:{l׮]E"O?~z\ꕟ_=Ae{իM&ŋ'LPUU*RHKKVTT*R9eDgfGnZ\0͆ ܒ\`^z :tРA...K.8p`ƍGq8R$9RP+9|puuիWAI\]]={:5o!B.E/ )0666?X+,,e^z]~=88 , Bu 37W/0 ?߸qcڴiSNǍz믿3""G...jg}ֶm[%&<<!p+##㫯R_}UxxH$zUݗ-[f|uwe߿JJ,fs^^/\ׇ+"""$$gȑ#`Mt7???''Gq/(UV=~xܹO<{RiVV'x3fӧOu:hD"R c"Ʒ%DQA Y5666666x{_/ )S~&+V˖-ާ 7n *(((((,t{ܹ֭[cƌ1)));w_1o>}paÆ榦8q*,,ԩSo&iȐ!@`66O8{pOi/^d-F#VQ@Q"ȥ|o6}mݺu 8:e333XVVF|@=<|)4 'x U<>$|!Hp_J}Rv,YwO™bǎxbQm0***>}h삂Xjƌ 5W!'ϗ8lFCo[rurå'?-Z ED 3~MR(Jnm1 Hxw!..zm $~\E :tѣFj۶`޽{jjjJJJVVZرc.]ϟ߮];'''eׯ_|rd{\C`^/**:tЛogϞٳg#[Bo߾m*Yf$3Fمǔ2N+m!OSIx 9a=_dqR{Eaӌ1hͦH$:u/p̙۷t4[2,88xѢE۶mkhh(|@:utE #p/< wQ |o,k46mڔQƄ"DdDׅ  _~k׮9r$ (++S.Ϟ=z"$Qtդ$wwwJ%0^ c|B(q"Y489 3XxV ZXΝ;[lۈϯ7§>}|w;v4{r0DڶmM!ȏte`+jā(Bǯ}#=让Έ5|WH\oQJ8"VF(H,a4|] p> D"l5̢m ATx{{#+I#GrgS@8/ Ìaĉ`tWWWD/l%"D] @b(lH>1LL/r0OH*! &X6χ7%ܸqG^#r0Fؠbv'0 ^k(*p9x!CZQ%ĝ" i>=cW-X@H 'i쵡CGx@!6}.,pPz$8% W)|H>B2Z n_+PHI|q㑖QAdm@tr $Z.i|OU#Kt>Wp$x#Hqr~WNL;ti|D1ϳDP$S*6r.xBWX߼yÇDf<~'+1# 1QH1hRb3+`sns㨦OC,ĿϕZOjv^E"ٳg|%0|B!NM NM,-rnOSw޽rVYlO T2 prrrwwe$r a#֥KJUYY N<ŋ @"G9t&-B)ļB | G7R;S/:kD_$@<|%#s X:@./'DV > HEHTD`-8;\$!<N.m{q|Ha!)ى Qx ͒ZP,5.^^-ꢀpr,T>u9 6zBeKL8jeHkqt { H8M]fcxG‡tx@?qɠXka|@ܺf_bO4.|l >x_rOJ|B9F[T]BaVqE]~37s""X^@$?x~BZ"j7;( ~6)K`[L-"'RGקּ|ɕcؙ36/hь02䛈ҧtm6 q2Lo(XҌ"ںdE _HMlj[*-Nqi[\ {iV= I,(p՗bFsB ]HaQ)Č|qhqk+D;x/=QH—^@pPPjaM7ޢ(F0@D.!( |BN߉JRo w} |B^o2z>oDgk0 }NȲlAAA=ݻ>|믿VTw"AR aV*Z9@CBB0ՕsѩFR a6 YYY;v¬j;t aʔ)K. 83?)oV|||vv6W*Ӄ) ԰0uww![,D:={, @ @BaooϝL|gggn0:/۵kG6>rBZ>x`RRǓg!lٳgcbb_F#G֮]g "<99yΜ9HLL|aBrTRR2i$3fP8a"{X#0Yfeff%p 8fX/^|!fy޼yO<(ӧOSӲeJl΁?`Is qٝwעV!QS&.]QKђV<痽[B ltlIӸrwYuH".M`3.$O#H$/g7ntq[lyxŊH]/ /+HE`0u떑akk+z}@@۷uۀ~A޽qeܹSPP={zjeeebb"dBu=%%s휊GeeeFFFҽAg0tVVV7nptttvvH$~ A$D"ŗ/_0FY~}xx8̇E$길gggHRRٶm[faÆ}jkklmmrСC]]] o`mɒ%mڴK.//D'N}0 ÈD p9KRlll&O,xYӗq^c'K4 ۶m3 O#H1Lqqq;v8&oٲE[4L Vy @ٜ믿[4eل~b j‰ϥĿǎ"977ݻ`Nxnn{8 Pܻwo׮]B(,,wޞ={X$7@III;wr&&&ܹb3w<))iǎ i9]v544_zO>۹sg}}}UUU\\w~-Q$i4]VWW_pn^Ϟ=Kg6 wﮭ;u%$^gϞLl4sCCCll,n6ۧRZÇ钛JD>x ^jG]|YT޸qC'ŸT*t朜cǎTǏc($/͠ǏjkkG}zÃ\WWwqJ%zwߵ瓬bccKKK/>fR"J?~Eln:f̘,X"!;ΝdgsPPо}uUeY6((:t޽;Œ ڻwohhhpp0X"cJߐ={DDDp,qNvvӷo_"@?ų;::fee1 VgΜiccChooc43Z3wZ8^D"\./--h4ӧOCBd*JT644L6ȑL&.))1Lo񆃃T*h4yyyf7prr஖J:.;;e٩S+ɸI'947nPD{u E||@+Weҋ/ L^QQqYZJ̙3]Thӧd7gΜQ*;wB3˲ϟ///(]wʕ"<1!!@W^ nX^@b7m<v*jE _0X^Uho!@0z-\%T _K6}I/Ca4^6/%Ԓ/{ /D N@UUU"\ܿ?77W$!Jܥjwihh`YX޿????777==aXlD*N/))yZ$joj .)| Q㒇L B\___]]B_ډ "99aRaaj+++ZmEE|- h4j:55UV+J>DWC,0pDB6:Kc"/HTfee듒lllDzl@@@rr+RQQQUUSXXheeagg0 vAy CfffeeeEEΝ;/];88<|S$FDD8qƍݺuH$EEE:tppp8sLuuǏϟ?߽{k׮O7t:]iiiQQӧOoܸѮ];?%2[_X ,^&1%/...**)---,,w͞={D"JJ+T*+++sssDӧO#-- _YY ^ VVVo۶ѣG...JRR'OIҚwﺹedd+ǏfffvԩJ >>ݻ:.--ӧ&VVVwܱ3 iiiȖEHALGD(j˖-51cȑ#7o|-aϟȑN:ֺr\ v(rrr*..V(zT.͇H$JKK@m Wd"hѢER{ʲlũ6LN5k x}555 ^_WW䔑j ޽0 |Im tMBnݺ5''aӧ5ܹs7nd&00;x`.]jjj\]]A222ݓmϏa;wxyy%%%߈D#G!!!˗/dVVV?L&xxx80''d2kN,={W_ tvvoWUUچo ڴiSSSx )&&\ܿ ,),;55U$0 뛛o2BCC&eZ6++ Aս{={QN^t)cll,bhhz}~~~uuZm=|ʪ}YYY:us0D*|ĘmeeV\ .߿߱cǽ{e}}}#""eeef8^R EUUZ DDEEEEE1 SUUu6m|7 `RWW^4{ə3g|||233juuuuΝZmnnnXXL&K$[[`(D"i׮]jjꫯZ\\\=(-- LZ~H$߿D"3='!o}}drttl1;c:*++kӦMSgddxzzM&eoE ݤz,pVJ^Uz%s—^jvE?@,[ц!vWD-.q8I$~q"> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 255>> stream xA 0 =fy9뀟\j endstream endobj 338 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 113 /SMask 337 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 20005>> stream xyxE8^3ܙ@$p+9uUuA]D||8˗={ MF7|M b]]c bjjoDQ+?h<<tGuVQQѓO>K/UUUuuu)))ruu=|MMVaZ_,[:?.˽***3f̐H$}}}oDZ WX-,,z}[[[~~O>Q555aAlllĂ_Wkk ޼y8>>_Dx5T4....\xΝaÆ DbpOoootTpv̘1o+pРwy'##ƍOjΝ~7 E.]rʎӧO?|… ΥKjjjƎ;|ބ5kWTT۷/++k̘1vZXX_򗴴欬۷o>|<55+**)))|իΝ|+W_~޽{&L2eJ[[Q^xJP(ZZZ~i^9sʕ+/<}+W^rS0 Rx`޼y,do޼ISdW^`^<>uuu===ATN6 UbX[[wޙ;wnBB#00:cƌ+WÑe˖M6mʕ?3<&HIFF` B3<GJKK9kkkj ѣFϺv-Jy5R)7fTTg}W_(ŋ֬Y#JSRR6mdeeuԩ^{WbŊ _Ν_gff>͓d{ꩧ{=H_SS#---p)66v˖-9993!ݻ.]*7l؀?___hH I`4YdD8UYYP( ;bss]P&*QVwttA%M4g}ϯ833s騶+V7o޼/eRRܹs A #){!Hd 8``e``a+;vSؼyk׊,--?Ç3fŊ~_pd2Yhh(7J$AAA666~&>>~Ϟ=۶mh4s3f q#FHLL<{̙3 <<<,--%[om۶#G/^sN//QF.YdG]b;::N2wߍ0aqUUUP;w5j$󳳳srr8.<<|ʔ)_~U`_P_P&6zh>֭ƢAjv޽}A|駃7nheefD%D^awwwo  iɒ%|mll`mFQ.A:Saoo@ "ARd%ZL޼yرc_}: _j޽{W-)QnOOBJ,E]T=ٙ#FP(ׯ^hj%v+hXc4\QL*"C&4w04Z555.]|[[[QZFb|)b1fcE.TnJ|*@dx2(j7dc5!**ƍ ߇T0 45j#&jj&#(4CU+5UQ)Zc{{{CBBlll ZffΝ;Hrl gTZ_$иĨϻO2C70L5662"(I$$H^߹smT**!勊,,,֮]wޞ!sZZZjjj09DjNHׯ[[[\~}dǎT㤂v{ U vvv]]]sˋ=3`;/^4(?WX!˩7nhiiY`hJe^^^^^^aaaCCիWϟ띝BCCCBBBCC---?Cn`7n;v믿C{K_W_}奥8::FEE655XZZ?~*//복 *J&޻w?$$dܸqǏ=z֭[CBB-Zh:;;*+++*****jkk333]]]9s愇S_b8۴iSee_|N"7 qN"ꫯYn.tb%̘SxCgN`#JbEHO2pd׮]]]]xi4gee*Fႂ &9r:44t֬Y3g TW\ioo={6Z`QwOBuJJa')F-1b2ױq2"bvQȒPdل&''߻w`05*""bԨQ[n9s%Kz7#!!! |[Ռ3/^B@`ttNknnOZ^iӦ*XӦM+,,T*֍p-T*ZɓIbҍ2^ g_/0RJ`-J !PsԺǺÙQTmmm؞5F-3ܹsg%ܹsÇK>&""b…(WWWf̘d8qEGG̙3  YKK˨ȷ~/o=zoڵ>>>򩧞6l`裏,,,^~e2#677_r _8Fbrb{ڡTԛܷ25]ͤ]$Byyccc;ZQ|EkDՖ޼y͛%%%nnn111sݵkSLL̳>+d 3r;vHAE GU]]sN*T*ݱc#ǰR(@)rL#hb&6yrTUDI QY OLHPtI^yJ'U f;WTT455ϟ6mڶmt:ڵk]vO?tٲepDS,1]%ٳ?Z-)bŝ|8oy£Z9f '|uI\ʏy…c/fA0R4** PRRnݺ .xxxU"C٭_^ !oRY`AAA\U{xxYZZJ"d x]í ~?ʲ0Td/3}:ÞefW !?9{}q7npww ڲe˭[ dVT&''9sf…2ĉUUUMMMOO_> zp۷og}rS"00pݺu^oooBB^<>|W5ӧ3 ͓ WFEEM<"hZ++nxK88F1PGH&T Q!H v!<8Uz'Ϟ=HIIR1clhT{nMMMllU>3ĄRbMeóg&''K?O|?񏮮/^8`0{Prss\+H6lؠ +G{{XTk.>'˗c&sd|9<(DRWW /=ztѢE؟a*H4*B= uPrP.!0[og c9`着_GQ^^p}͛I㜜klmmớ: boMOO7oޞ={BBB!#F0!L&O>=))_~뭷$D"ill{=5IjDPev|Ȧ&8ncc8~M2Jq3 11hH PB<#P_.8~|ŵpDT޻w/&&2`ƍ...V<^^^pE &@^IHHz믿饗㸸877W_}Uiii<F=.Ċ %C?'\x͛7 }SSS]]][[[oo/GD;v D M1i'l.nbIRq* 5*ehd2ѣ9 {%I||<8 Gŋo߾ R728ȑ#3gΔJىAAAXY!rss\-)d$P"cd9{)))݃[o_@c$ Q\I@Ʌ CakN YFĤTr>Ǐ)!e&#aܹ؟cJo8nŅ 3fԩSoܸacc3{`FQӟ$ ?B-L3ňʊ w5uT1XdF 5TL9!􊊊_~ޞ̖~#Glٲ?`( fggtMe\CD2mڴɓ''LⓋU*›IϽ{:88p7g771c,[,""^/b#3R,ʄA6ـ+kkիW'ȵNiӦ`hB~MHHbW111 ƁI P(EEE=:(l9RUU닮&LP(_>ydNf͚_]?oR)b_R̋bKm󅅅nnnʘI^rbh܆حguuuEEEgg'+ :rӧ=zrGyAm_ jdr F?}.++xf?J>)vI*y(97|hnG.!7mooOLLtvv>y$#?UYYh"3DOŐtww8q"&&<;"A|y7n_~…W^d[f&}F`2&V6ki:;;߿?,,lٲenq+ح|&ĬXͻ0 `޼y(!:^_^^w^LАP(Gvvvvvv?`q]]]?c||g}@*(b"BNyxR6lPYYyС04IH-]&Cf2%&iݸ>bl9请tr>3$g >aggߌ'DN\ZZzy湹hjugg'RՅ;?IFcKKKVVVccի}__@>===ɾ Nh4mmmmmm---jZVxzz޿3mB_.ɔJeDD>k&+q'Oܺu5kvލEiEM>,[hJH%!qRT&Fwliiqٳy;1e ٳ׮]ۺu2z'++AAAV 'J-,,,,,#$NNN^^^ӧOOOOtuu]f &'//ڵk7o8n3f̘4i\TQ[[[]] ammm4+++ CkkZh4r+::zҥɰ`S8j  5~ ^o4R}}}{{{Q/a ؜ \lvmiiO]… /Ud#XjP&"r&K-d* 0|ddsӃC0B+Ü9sz=@T!D{ u:Mpp0|eܹnnnj'u3`4]v9R]?vym޼59288811oWyYjuv???4BQZZ`mm-˭zzz{{{]]]<==JF222xK.wPIw5۶mr@;YuԔ}ÜFu6؂Rq0jT=quO<Uon?l"&&Vy  ;wnPP7[=//_c"ѡR?$&&Bf̘§Նg7ˈ#ÇuuuG}}}1OOOWWWXDcǎ7xQxXtǏ_VV+SN(@xig ``y1۩Q3BaՉ s Փ: RDՙ`Rv ǥJ1$$$$$dڴi/4Fkaa[o>}r'liiٱc뽼;vɌM5G?4iƍ &L;1f#dDkhhP(NNNs28 Em޼Y*7i8͓Tn$˃"T q bIyW"899}waaaRKfìY6nhggd|lA"1zzz.\?Ο?Zeee%%%0uV&mذ!))7߄30 @(-- I04th4c0zzzryMM |ڕ!iӦ nDF: !*r'2Tel3;!d$J$8|@CCv6 9r^?~~Ietׯ_?rHQQQBB͛###uG34wss[f |©@yyyiiimm%0bĈ?|P޻woܹ7oބ/-///..vvvMKK3RGGGW?]"<lI`)D9D"sss>ccc EGG_VDN>ekk7?><<#D"W1dPTTt_~eԩSa O\\\K#``8suu}!Tl0777::ZT/z {JS*p0))СC|I0rAwfl+3F "֊%GN޽{:$$T__?bĈ|%==}֭&M"NRj rJhhFٳgσR#&L0nܸ#G!ìP( FǏ={_O+QB;s SFi f#)SL@%[Ԓ 9S*---ϟ֭CovJ$ꉙ+B(c^S?$vF0򊌌,,,3 eəÆ .tssOq $...##CP/8R(ɥF1,, nԨQ'N3gΜ8qϟ9s ۯ5z ^&cH/9 :uBv  000TꆍW:ǜ B5#V9m ?%#mll=Pl&Ãz^Frtt/rrr>\RR 6a„;w޺ugٻwoppfy!Z " `z05e1r>R RFL1JꃒP4ucU:.Fh^z(D??Q!!!7n܀&ㇹF'@.?~ܸq<''端ڱcǰavVFMPAK2ߩb1GQ9cIqSBYVy?9R*-=b ~,ũja>69;;1cDGGϜ9_~eO?>-TeH̭lB1B9@ YI%%L&rww.vɃ(5wy!w]Շ "N3"#(gxVTO$V(S* Wtv*Y77SNYZZ*7| ejЌ k@&C;U:RGJ(@^"\t ^xd6P+U(j@Ѩ`mm oƘ6EM,IHFBxvQa„ gMcQeW,J4=H@irPhYJ0Ȗ&$ц w̙3ȑ#cƌBqDҤ#Ft:E &-$Kd;h ҉1>t萫kss3#d)恘$ATFP[D!zFlmm` '3`ܸq$?CC1L$i۠l7{0zxjFcggo(!ܘ`3+i  OӋ9%ҫh)HG@a3P\.߳g)C`!QEl$ЕRj2y)K &_2&ԆKR pEn8H?O֣a z,S+D L,@ؒߤBI&D`擦"`f-$lSR7/TH Z;vMS q ։mǥw{]H^gIBR+Bm9@9S0Hq(C14* Uy1+C[!Θ4fhԈ)&P^7@f`# fJZu< lܯj$\IdCI8%\&,ɧ9-- >09)ƁD,gԶImdfDP0 MIm/_,;;`PE6fE(~ xY``sb~'[,%'I*m)kjju֝;w좚#d2v,T( 4aBʋ1=f)7pfDp9!v}뫪"]L=f` ]$ B"N[+["G8q"|ԢE===T"a(c2 qbz[[!UEqR" $H${{ qF 'P6C13 @lvttX  P'x*aHC%HA߶F*ʑ#GB^xBb9 ~׸eHA3%; ŋc`n& ](. $|&?Q!'d2CЭb6-73@t8@L1oAtvȚ7%"3B̮R`et !hWܶcq!uM95-!y<H*mgmЋ\DPXQiGP|hLJVqE-Nqn@6ֹ4%_BU D5 nFΠҊ1*K0D!)& c )naPHBG @`cP-'Ѱet&3"#*K=nSE`&01/j8R:ϟ]TyndԨ> [C-Uy1"ڢdYr ?nE ۰q,lF&JB&ZhԆ` -(C !8hm\ؒQ#S L%gT8AT :ر OZ`-I(RIlRg(ժYa2*l*3i2V!l&hB9" e@u] * 3V1Q QXR"(Ft yA *uUA$h Qd+S& l>)1 o+!&"@6AC"xkB*.\)v ]i6°fBΒK!#FS j(D5DL<`o<}T Fu# 砨L:Ƚ*1-6()cJAܶ$f߂96Cy0ؠ𝞁/A5ݴǻ GY?NR__ѣGcS"gRῒ U}z`i|e_F8U.=" j| _BĐerxsH)C.Y$55u$11СC  Zjڴi=T}}}QQQWWBVקj~KcwпF qpp())# .uppXx1Cܞ~iNrry87n^v-իWf\rbधWVV //`TVV~7۷og}w[ne8p`ڴi! ۷o=3ǝ9sf˖- BRZV-))6V|(?Ν; 3g8pΝ;TArC|{U555H0 ŗ/_>uOKWT={CUUŋ<($O>!?<88 z}bbbQQѸq$6E<ܨ( !4!&)))ƍc 7|k4mllK{{{x7^&NF KKKGGǴ*hJ...---Æ #q8SZÇ^^^T0iҤ:,Jjhhprr1birPTAAAF"dff7.55IGz(~!_D봴41 Q===ǎ+,,5 ٚSAP޽wa }7 z}{{}%%%z+ CGGh4Bax>z`0PqڒP^O ֶo>Ңjlnnvڑ#GJ%#|R3?@Np]// X[[ۃ_ i?]\\M6zh*D$-;;{!!!٪ݹsg9r$[C*y<9NB?>55]]]NNNZΝ}v{{?Vp¹sܹ#!.ct:_]]M5D_x>>>0 ҥKΝ+))`4322Ο?&oooxꩧ^{5Ta2LJ$L&JϞ=xblz5v}Lhmtqpp8uI؈`;::yZ1رcuuuOFB EC8uRΝI3?սth׿vmN7aggS ޽{233 ĉrQF~?I&Ϛ5 ˟}4t111vvvbmmm%&&6..}ǃzСckk qP>tIHHѣGN:mwppHHH 3wޕH$/_NdD7VQy&.\pvvwoֆjSM]VrwwX!=IIIuuu-))ydB___JJ[qq /ovzX՞9sF*:;;M%%`0)D5Luj%,J_}Uf HR{{{//^x߆aBϟO0ܡ:;;Sd0"""Lot8.88Xь?>** *;u@t4lذ'|R.2aaa3cǎŤ222?Wɔ|N۷s̱ԕ{g0ZXb Cz)\q=<<ȭ Մ~ڴi'N  N<9~0ZD$3gBBBƎ[__?f̘1cyWrFCXtiII %~UVVzzzZYY yrѢEMMM| ,Lz̙3Gt:F6iqqqRzM̙33 !0OIcbbBBB[&Q,V3w܀ W lrM:ttt|wgmgg}ƍ@ln[c D ? ٧[ظHRq"#Fj#QYr0*a|H !Hء-ӤQh7' DLmqG P̋gT:-T#sQ y$C1IBc8ȯ_Pdbd(!&5y&!` uq 0(իEI$"Id 7Hk#9_~C#8,_-R[ѯeojjj<8>xUT*JT2XtttC tn8`0ܽ{6??㸚'Aףvuttdgg?|H]@TƜFu/Q4pclmm7.C䮮.Nq\CCCvvvnn.s~{{{__dCooJ"7oh4';;8z?*q.ꦦ>`twwcGR#w ]#6m[{V6/Էkkk Saa!2l߾}<0a|Kcccee{322jkk;;; &JyA------~+W[ Eee%<%%eĉ)))~~~cǎqFkkkXX\.OOOoiiqss+((?yq㲳}}}o޼P(oݺ9  X60p0!qTZlkk+//U*]AFG5jmVVF)))qpp(..6 A[n;aaa^#X]] veee{xxH$ӧOO:~rrrʂ߿:22~...̌~{NN^ijjh4* ޶>|p,dx {G%e*{?ؿii)g>}zFFƾ}_'NX]]cmmmmm$,,,T*UsssGGG[[T* tˬe2`ٲe!!!RRxÔD"quutR||ɓ'}||Fc``T*겱1 555>>>ӧO/))섍VBlM:PB\!I)DZ/^p0nܸw}W&{2L&T*ӦMjժ;wz@MMȑ#KJJjCxxx___```AAANjggw۲e!T*urrtAAAEEEqqqݩ򊇇Ggggjj9l[[[lmmcǎU:~xx/| DxOnQhA?s}WyYfErwwW*naÆuuu)fX_un^x>wqرc/\骫ju[[N Q(666α< 1 NNN&::g͚vyF[3rtҥKjunn#GYWWn/// L]*CBB|}}%/pvvkXZZbƔw8qm-888--㸪*Zhmll`GQSS~](E.`$$$\|9""{Z s|p08|H8Fov2!j{-D"πzzzb&"@vz`߱V\\lii9uTT$ ᐟU*-^g*&ޤB(>OB3Tg،IPМmH`9Lz \Y[ c5&1cP|AߨXMTMfl1UٻYzB"{" $OK7cJԠ $lMnٟb I%d :.^ endstream endobj 339 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 340 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 339 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 41263>> stream xw\?>z4!"؈%h4[,c4hhQ4*(" (H* swݳzy<<>gv8q "`xȁTP)&&A$ d~&X4&8,)QQuZ ".}TTvuuADbV[[[^^TTT~7^vfmmm-aRdeeeuuuPgϚ` N8!/\p}΍7‡+WdggGDDǎST\z/111&&4..cΝkuuL&njj*--Qmmm*z2憆7n|$|W"h۶mǏbqCCaRYYYQQQgggkk+4^$AW777K$,TaX,.**ڰaL|rXT666fgg+ LVUU^__P(Riuuu[[aXgggii)Drfav`յi& `ɁC| sܼ\T{xxx?sذa<=|P"ttt؜;wӣG^r~xxxpp^[[{-PW_|CCëWvww:uJP:tHKK;rHKK=<<>SJ驣3uT>ogggkk{O?444tĉeeeмׯǏ뭷v5a„ѕuuul f̘T*#""N< ‹/zzzܹ˞}D"x񢏏ObbbZZZ|||eee``Rwɓ+++ry[[˗-,,rrrlmmmmmR-yB%|SqqqKK˄ >}JKK9RK,--kjjpJϟё[WWwT@QQLP(233߿eff{YfY[[>|3!!811,44СC&MڳgϿ|rPPr͛77nSRRf̘i&++7oz{{:u ðð77gd_UT&&&~_f ;w… 555Ϟ=kggWSScbbRVV[Ž_~ӧeee~~~aaa&M"gXɝ,%JR鵵hX[[[[[Rm۶gϞQF988m޼y֬Y׮]`dd~zboo޼lڵkYYYuuuݳܼy3:::g͚E?!!)<<޽{*ߟ pŊ۶m333[vrqqqqq9tʕ[n޽?iF5{l̙3̙󎎎Jcddm۶s疗[B?0455sKKKmmmSS СCAAAo֡C~嗳gLX222 E__ 477_?Nbf\WWf͛wÛ7o̙C <<<֯_f͚5k_v!//e˖}eeecǎz~> jkk͇ F=zڵk?裥K>{ رcɒ%+VzG222n߾qƑ#GB6///'''gg˗=ȨXx-[ꈐ~{֭555?;w͛]]]kkkuuuy<^__L&ѣW~ ""⧟~R*<t8kxCT:i$49mڴ[nwvv%%%Æ +((x7[ZZ Ecc#FyDS^>Oncǎ駟(ux={̙C6#y/aNNN˖-~Ç_0h'ɓG #RH155nhh:::Ӄᳱ͛e˖ӧGGGO>6U*)@{{{{{ಲ8w܈2É,4FFFz+++O0!%%:qds1bDjjj}}=& ύjjj/_P(oNʇ;a„'Oܹ355իJ\ 9| OOO孭0DWWB )3&MtǏG 8 :@@7=%3 g͚аo߾;w={ 2d͚5;w477O<<<:::BqLJ r۷[[[Cfmll͛wݻwwuu]r,11qnnnWWӧO_paٲe<{{{ݝɓYfFDDlڴRtӦM7n,--aO8d}}}ǧMsN ChiiA8C Ԅ!zzz6lrСCq8[[[΄nڴ)))iŇR(>ӦMCze겴D jhhoڴ zaÆEEEccc?##9**jҥЁC ?|pxx+B)""nǎ˖-KNN311ٸqcMMMUUĉ! rJ$--9;;'AAAVVVv  E~~>7-,, baaaTB啒sN>:;;wttXXX@3z'|rС'n߾=00J&YXXPq*Fg괁dJLLܾ};(ʇM)GSY -@v?Í7~'OK.D"Z{GK0x<8?"E S$&-@۶mKII?; y뭷4PP̛7j'L*(h": ͋Aw RZ[[?3naa1n8'y{{;::R %_[/w u*HOO7 hjj0񣶱M>ÇsT?Hğۃe7bJ Z؉_"`I(DRRuꔯi+**zzz<<<˟?T*wQ[[+ &L뫧-"@M;j mc t%pQ0Dd٫e*bb#J $kGd~5B(QhҢh@YYY9U*UVVVLL̽{gϞ} GGGRW_-^(ʊGikkM0aܸq555˖- jR,La@4{/x _ٵpB1E A=,i `%y_5`{{'OƏ/ݻoرА6j(zj :;;322=ztԩ6??_ĉs555522b;==($$}̝;/΅)yozɓ'oF,O2eݺucƌ!NH|>l̙3gq\P(!!!%%E$b' -,,,--yyy_~ezzzJJ <|||&O<$OPzqooo@@-,,cf q7w)225qDcccr,UUUFFFBG*3D0 [t)H$R(>T[455Nk.GG˗/kkka*J,Ꚙ?~ҤI#Fe*3T )A%%%ǎnܸ9zLݻ+KCL~O/Ř3gdggl㸎T*2eӧO{~mff&ඞR"""V^Maq4>>>!!m„ 3gljj:r\.߶m$(Zfff.f92##(x@ ͉ ѿL&{.>>ZZZWWk a }ĉB011deeI$T?WHH9 .8fZtTkh4Vm~DW )..O0!66VSS-XB###333u'O 2$$$ĖF@XXؒ%KYrb&R۪Λ7ʕ+SN533xw?}4///!!y8lذ3g]xqd"mmmOOO9͛7̙3o&L,J\]]KJJF xxxP*!C0d~49SôI a;n j ,x+``CCüy󴴴*++===!/Q!i=FV?Ϛ5ҥK---iiiJrʔ)+V8zNXkk7xC-dH6i$ڶlOKKKJJ4/dz6mqb\.?z@ ҊYh.j-!p@%OQ o[pܻw=##q8:9J.. TA{♩(Ȳb!ç'B(+ oܸ{4r\T*MMM> O$Dhhhxī)#'SM @KKsΑٸ7hqmm%K޽ѣ&MڰaJPB[[{СwXҚAG=㸻{GG\Z R) D 0̃ZLKj]LT@J<,D>ej8əd)vuu}!<!0,444 ŋO8ex;@BQ ?(\^QQC@Bwڵ^^^_uFFƌ3v=u^1xOoGGEGbOhp.Li!miUe)D٩T*Ҧe;v?:JyyyfffxvLSXXh``$ѡT*ՖOs̙3gBcҤI6lV.vh>NG2LWW@KŦQ5zQdd2@V&GSkjj233޽KB</!!^HI8Ϗbرc0ٳgϞ={IOO5k?S @ZP3ׯg6$`9x!L_(@2_ǎ@P CCCQHKKɉ|&:#?~޽>H[[I|upp l߾}ĵ% / b'0 68q"22 #jTD/^=MMMǏ/))E*\Aj(vttJE2J._+w+ׯ_W(,i)%㵶cuWu m(puNG.I$FE"Qii,EZ=@ P*Օ555$333 p?qĹs|}}'LL‰g"˸X58i ̟?;rȹs8hѢ5kXZZҖSN͛7t 4'$$7bĈ x3A6YD P%4LNȁ(ܡ6FP(-4 疖&LXhTGzxxϝ;O* B'''xQEE_=jԨ B}:''5 ERʈKi픨*0͛/_ѹr__ߣGd2ˉ=&qvv5jԌ3LMM'X|ҥ zzznٲeȑJL՛.#F0MMMgϞݰaQFF… 2)UR(.ޒCrTɯe%2 A .QS'NheeC=zo_7n\VVָqp!Q00--ðkΜ9xjȐ!SN]j<(((00ַϟGDDܹSSSs̘1bXSSsϞ=WvuuH$}}}===3ZRR2fDb 777---mmmmmmMMM]]] ===@ܹP(6l؀g񏁁 ֭[|AH~P\80CKNN퍋;+**||| ύ7<8m4b*L! z$SI7FbD+$ʆ d={rV\PP,--$I~~~UU QTB[IhKVVV~R? ~뭷ZRZ[j> D'NZXX|7...p _OO@OOVTܼp¡C»e2\.J CRTQFiii566Һ6/Ȝ|>?88xէN:tмy֮]kmm]RR% )]ѿ_q:7nܨ B?~,cbbˉ@~¿jjj?~ٳ[nW_ݽ{^1ӧ γg.ZwީT߼y|g egg/]tӦM~~~IJQ]]sى'.^8///**޽{2:;;9;;ÐqVk $쩘:WMz$T*mii!&0`ƃ~]kڵp6LT^y.10jժU}}}7nI,002rϙ3G*]6/// !H i:=eToPEEŵk׺)Y pb203Ԧz)FP\Ryd999,lBjkk;ONRPPp֭7jii(8Cb:88Q444)-@J8 UTTX,^lYWWoYCNJ?wQ8?gu'^탂Mprrrљ9s&|%'N ISSs۶m3xѢE/]4{ӧOԅ L6mС ¼lbBPBiQf0v WPB.&bj$Pd%9@S.iaݻw$W;q0X_^z՗/_vwwG bTg/NlJ577(ai--*!gKIIImmmGGRfLCླྀ#-䴀8'6oW5%L0Ph@M((=yyyX)-d}VVʕ+CBB$) @&YYYy r>55F;AOO?#L"7&&ٳ~B b~`ff& M033kll"R(xZC;h2ɧPlf1b,rg٢mk)(ZLMMMܹ# *Ǣ@DyU*[nݰaB=nџ֙䴖_#WG͝;əL9hnn~U\vtt(CCCOO7|#!CKV.W*}aRloo ?٩Vbo=hnnbhhhddqH<ƍϟi G1 􌈈 .422600:::?c~+*TJ% yX===AOO4~>oiӦM=ta@0?~ w XXXXMMM H-}H;|' $$9VQ`jدHHUt;@&_Q;v 2QE\|^@JfEiy(*D7o @>Ci'0a„?XWWhB~ $bb K&2 .|*Ck YPJV3UN!hm۶m̙D%0k"H)TKvJ,dDz|vvvvww'/4fӧ듷q\av?$q11%%%-"bKȥp1Pڵ ^$‘::bĈ\b%^(OOF6+W֭[/{ӺKRՒWLLLmm-i $@v/( X @wwwyyyll,GKLVQT*JVt hZOˉG!^,X ) id!|wL&ɲc}YhI, E]^U4W BJUVV6t~'_)FGuuuRRDM'˙qtۯ +ד.8??޽{D emlkJ0JuƍtɎRL&06xeY`7һ%*55uLzi!WTTܻwc_b!vs]8G(C )--eee2J[N<_ڶmAbv\wׯ7n\HHȆ s%%%UTT RvvG}TQQwߡ]4dRrA;gΜ WJ+&GJJNNϷl幹cƌ8^XXH|CR269MřR4/bu^^Y__@./2yyy7o$wf y…20#DH$'NߴiSddH$Zp޽{![ggguuuMMMuu5\$d]]]C?H@P,el$L!2YZQ) uqq1ϯ9T*)K?NE?HL&EpZL6Ms[xv6H4d MMM2%;;{̘10}HHƭ[X[IOE >%\o-[ eMKK{ȑ# fSSS#GBGrrqmmmܹ3|y-\Ϗ|:ܹsҥK EZ꠵׮]+**ruu/f uGAJDڙ3gΚ5 qX\SSTTT4ydwߕY@ DDRui~u#[EAAimj vZ h9(v;__YfO2ngȢȁMMMEEE:-[<<?gΜ[[)S0 (ȯܧH;1A(؝ZBA>Z,8 lnStƍ?XSS~prr&w>(W_}5zhaXllbccF_(^0,99ٳeeeK."8?04iҙ3g6o|…C\vmǎ#G\`iYRU1vww;v 4zhx3=&b{Jox{{}ebzFPt,(ru :PifffvvvWnkk6l߸qF U*U|||vvD"MJJ\]]/]D>qOOOٳoܸ1f̘m۶gٳg/_~̙SN:ð'O\vm߾}...'O177?gaaaAAAqqqSSעE<==---1 ;{lddܹswܙѣGO2 A4=ϟ?d߃aիWG}СP.U%˅e/R[rl?fߺuTˉaXDDDqq_~aXYYٓ'O222rrr0 wwwmiiصk䢢Ǐ'&&\r…oZR*bG@yy{QfH?ӧy<Ν;E"7o޸qѣ)d2YfffRRRJJX,rرk#?&'''''j˖-&&&111 iٲe{}wnM܉>!L"&O /T*-..|kjj,,,V\+++}}}===>}SZ핕C c)T*qQQQoON[ҥKÆ 2d"?TOO` 7]]]3g+)@jjj(\J577',D)fffׯ_wbMpc!bbbbBCCqӳtqqT` Μ9=4&L(,,g1|U*yDhBB[m4RۭYOx*RvH/ }MII INN"<"A:u*vrR!!!V***'dS600rʕ+by<)7N(444%%78vؐ!C/\c %|@ !ڌg$$$KRڂBvj6{]0 miM"ІsH+M;9@&'''CCÒ'p͛i"jhhQE3x޽d%VŴiӖ.]JAkyx<ޅ  k lll F 9s?{o % qc>gΜ'3XZK0rHB3y䚚hѢ;wP 5ZC- 2Ҧa`R;;b{w (kiid2pZՋ/>vdzw!gΜD}YTTԎ;0 ޺ukHHHcc3g"##|Mb0 3gξ}XnKLJ==DFF ~Kҵw\wJ7666}Ņ6  ',#u& #Glݺ766/lmm׭[/Ku58!88;v2ox=Mp1HaCsiSq$ 666<ҥKִ6N`VH$V(|Xo8nccn:&QU$R-dA3AVkKZSPӪd ,,PMdT*KL $+**hdO6-CqqkWWj9# š8`F8^XZ  +S.sHR'Y\vR`m_+? QV3((YZn?|p͚5\6+h &L{I? -   ,mreIr$PtQbXx W&J8 )H:&I& ˗/X$+.Z/xѥ \O |y )( 9 ǘ+$KKvB  MO?D ᘊIE+KGGEt#\ C Zi{-T;"“,{T`&!dQ  vW&72% |fv,f:p\q 9(e :BK{*B]YYYppp^^ĉqqq555qqqĭ+"cc㐐 s\\+7o7Rsq6 _sssꤤ$BF"4yyy9qY&ʏaXIIIzz:0&0֭[ZjhhHHH b?H+',QQQE"QttZtdQ!;77͎Z QQQQb#˗/7xz~Z{n߾܌5 ZZ^^Ύe`_+S`,u .NV̡p/v 0^R +hzhyǴIKd|%Z!\$P%ZDpP=Z ^HU-\BZUY}0LA ހ 'j@JMMmjj"3P@OKKkiihgZZZOOG'OT*(}}},=JKK#!Hh3W,HD"Ǵ ɣR۷9 sG,J233LRRRR8ڀa؉'Gyjb)Yd p iѣGRСCզhnn B aaavvv,z]SSS̽/^U(\߿_^^8dvNHPRR xb^^n*(//?DB)%%ԴёLKKD)))>>> ϙ(,,Ã|-EEE>x`ᔳPzYmmmRR/B>|RTTg`JJH$qss$oAYȤI={e!===ggÇX6113fLJJʨQԚP(?R9 HuuuQQO:;;755蘘3x<''N.f"?~?OyAC x񢾾;T*RkIeeX,GggH$eg655 7n\GGP(L1[(&$$;"蘙9evjƺ2*… 8WVV.4),, ð^v88ye7ٳ{ii-,, 񂂂6f;w0x6B%D"`㖖f%HK9998h8~]ꊊ233U*UrrZ҇b.(*++ S-mm-ӧeΝ;8766#H޽Rz{,ʀxSSS\\\vvӉFoii͝>}5eee3ݺu+'''88X(_f2b~O/vtthkkKNHH(((ʂ/?RABRF1uTuegggeeԌ=ČxNNΓ'OOX奧?|ҤI RSS+**LB\Çϟ?6mțŒ䒒Yf{Dg4>=5 OIIɥKd2ٮ]ݻ"P^^ٹsj'+**.]Դ{nLݗ_~ ^:SP744\t/k'd斖;vk'3K.~gٴ BD"џ}<&KŔYh &&&::: ͭ ׯ3f nܸ;j(2ׯ1߿-n߾}5OOO8D KsNddgHHHQQ;aÆ͞=ӧ### o!Z=!x@7dzEn߾gllaq">$&&(Jsse˖QHҾjW`eh$a">0 _zr"Ceׯ0H|8$Q;}2-?%l!L*Tˠ0,$p :0NbZT KGC R)C-_PX" t"Gd_Yɢ,%ݖ ZM$ %r Y(Of@2xcccbS|ZZիW555nݺmddnggGR U*f2j={DP(PHFFF~~~[[ 99>))k蘔/*999"^(fff_S>L>ddRǔ#,AJT{{{qq1RSSmll\]WWw----Ǐ{%溺:cccmmmNYx8r@0 .**~L&kooҺ}J*))T $%% BR xXа`f!%"RVG-X'!{)++X7UڵkӧO/..vttD:::uuu===r[XXd7o P$ njΒ<396L9m۶I$@CCCccq͛577o-ZTPPlx|>_PX[[+J>/4EPLuV{{{mTTR@"333-ZT__?mmmO:::nnnyyyÇDb\T*kcc@ hnn0L.WWWx)WZ!h }[#ЁJ200H$WWWWT>yn+ՅrnƇ;iG&!MMMzy === USSڵvvvphAARvuud2b' ‘RmmmɻdR311d ӓPfC[BQ__ Z*67}w\TG]veU:"b`}wԠ-ϓĘ1&1k5vaYX?03wR4<393e` vU |Rl4AY[[r^zW8Rj6b?_HRg#dw03rbM 6^G,G'-8>|e. ^%2 x҃F̥ucU#MB.LvW|Ґx!`q ~$#! Qr3 qqL. >NHqէO9;;;::syrrrkkkR)x[]]'J]\\D"QCCÝ;w=$>xf0<==E"V53"*--ljSSS\h|rZZZCCB|}}% xËa\r<0Ʀqp8|kekkkwekkkϜ9@PvvD"jNm èj!N3xSF٦MD,HP8998q"555//M6r<66655533ѱ^ XK0L@@kXXXjj*H߻wo.]ߏ?~\:99gQ>Gx+z~ٲebx֭O}(J#G@욤8Т%gv 7oޜ]VVzk׮999%''oe]8D۷hH$oq)RQYYy]ew'''++KL&W.畕t] Ilŋfsuu^^21cϹs|M{0`@`` 0f/\dӧ2gLL̕+WΜ9#>%KGGG'$$k… g^tI./_|%%%˖-x񢽽W_}e0>S???\խ[˗:twٷo_5==]Vڮ\r۶m'O.++{6ls,˾۶֭AI̜:u}Fh=zT[[jժÇϚ5k^^^ bݺu3gҥK 7lؐeEpW_}~ʕ]tի׀jjjgϞB [KRG*;/00pذaŋzw;v찶~kN7vXy3&!!aǏԩSCCӧO?Cooo`0L4ҥK L0͛ G 6lXhh(˲~!0aaaÆ +))ٹsBG 4rHǬӳgbbb.\q7N0رc C_jJJKKx{I&}aaa3f_8dȐ8p@.k;rHΝ;fmm=|q}:.//رc 9r$::Z$yyy]t 0l0tݪ 2SHuk4'd+>|ӽmڴ9rdquutz~6mXf (۷ɓ'E"QLL̠A駟2 cggWQQqҥq%'';;;K;w,;}t^4iƍ={?1 h _,\pǎJDDį{k?좢>30t_W^=zubb"$l6/_|wᶍZbѣ8O?͜9S2 SYY}A?Ν;/\`0 fΜG1 DfĊW>M:Օ/.\6lÇWZ{t3gO?x𠗗W߾}c0F5ad̘1cٲeϟ߳gO```>}vڥ룢6o|ӧGGG0 ӡCalܹܿsw1 yyy}*jɒ%sMHHtbxڴis7iii}y&>}#bbbLy)٬j ȑ#7mN)Gϟ?vZ.^yyy... $%%͝;w2ݻs޽{ǎ)b R^6'FC ԀqS\<==쬬\G >/IiiiGYlT*=zӧu[s +t:#ץKbL&aǏߣGD"Ja RD")N z}Qdd b<$|5_G^TPPjat:]6mLb2?I,h4٬<<<^ 8N~aÆo X@~[wuu-**t"֭[Ńz*w  Dz͝0Lddmێ; c#<6,U Nx 43 :411qݺuw%ɓǎ˲… q!\9ĉfyɒ%pzׯ_'Θ1c}ѣfŋh4WNOO&ݻwae˖mܸ-[rOh"{{{n77sYuvv|rAA|7wQQQ .,(RW_}y.]P!x(eH/^r>`nȑ .ܺu+0?̙3}}}W\ɓ0f<,Dӿ{{nݺ۱yxx\reҤIyQQQii+WFW( o߾a-Fqݺuǎe2ٙ3gWXqKwIKKKII===ϟ?P(ꈑBO>|IBB³g_CCxc'Nrss䠘[@W\Yd z}~&`0̙3{^^o0F#kZ8ck& V >|ȇp\*XF1xɤh@ pF:TSSsЬ 0۷1o3/Æ!+WRF#0,8f 8(n /*g,_sNNdjpt:h Y /Ϟ=yfMMͧ~ '''O`_`0jjj~7J$|#m!T V++}q:H<]#D"8`0"<la8_XfNV\2{l肂;]vgM"N&чݺuPg$L.|\@ylHP!'X ߅ N:q:w\__/2&更dnnkΝr_MziMZV2"ў-G%t Ѣid)1~cсͰT &O5K'aZ&S÷F$<;9 @I?Us\\qhE e.'?TH|'o .|`\HJeMJ\I㳕O Mx;2(fX5VktuSY࠿0_h6_KJ/37t!y qfhEht-5 BZT SQJTSEh^v zBN'JȀ'VlȌ*H![` 'zѕ!D]3DHVK3Q<BDfdNq0#4 KP q]O-I@CHÇרDWG(D"Qx+Ϗ%DhqH֊V5{ (Ps^HL-mj'H4N8q۷mmmG e w9~!feh'ǺYܾ};//ӳgϞȰoغ u-qEe}۶m=<< ̲o߾kʨ/"NX 7sjz`Kye_/y^KPG[$zR.^X__sEnl{B p8OE@b%_:g(` L㱐l TSS߻wop|Ox,~K@-$BsBی1\k׎x7,b[%nIEGG\pK@8x |- My $Up(qpGѧFK%5i a\-9"#Uޒϙʗ e%U_zLJ!_ @`37YXi袻-Y yO:e0*ePQ < '\E kƛj! GoS W t,D8 ݶ Lm۶m۶dZG셅[C7dj9~ ==+0f|}5q|hm[թS^zQv7. teee...-Y W=`6LQa4'L{K.ݼy3gRRR***F#IIIXR>W0PA!jG="V~<֭0òl\\?52 05 b/0ѕ5΃$XR6`D0@D% .]ossB5@DR`F.rooHHcǎ\Dh4R߿*GGvuرcǎNJ2..uȐ!3 s) <pFٶm[XXxW '9.K8ڵkrrR իWǏ1cxyyA60{a<##|>y[4Xp!8?p!efIIIa._RRRگ_?!*8111 BVoذ!00VT2ޞKa//_bl6T촴˗/o޼T&tԩv޼yGv4MCCC]]w YtZ~wwf( pSxxѫ Q  Cyy9p_@@8o O0޽{cbbƏoq fÇ E fΏ $kyXGw1YYY;vdW^ҥKjjjEE'0'ONHHXr%e7 /|{ aHb1wܚE >L򺻻'd Àrrr=<<&N F`dkkk=}taa{Ǝ\QQymp̛ZnhhH$ڲe^_rرc}||\]]yjz=zǹ21 3bĈ²2^RRRH,|8=%RKmS3>WEN&cʔ)=zWߧOiO (JǏ_Y[[GFF0 22t;vt֍;%deeеk׮]ƍ3bXqvvx"2@emm zwygΜ9;vܰawFdtEEEEEEEEE0&Lܹs۷o cC'J+//@q"WI _<8%fDn/B=_A#*I\^Rx-Go||{g b-JAAAaG>x;w >};::rE:y]va pgELLp, ܇ 899͞= Ds2mmm}||zͥ߿ȑ#JrΜ92,33رc999eeeRTdeM|X? qX5`!X7;w0]b)szJ|6 ƒg9Q#Q Qg\;Tqf`mmM C w:uT^^ e]]X(RGڶm;k,ggg O>d„ QQQĦ[SS3mڴ#F,\/|(J#Luuu .ӧϜ9steZmQQѳg233333sssCCC׭[g6ٳg%D}%%%}WK񦚻FL$!pkD%h!`B%#HD8 .}߾}>1bU7ݜ9s~嗞={nذ~e2ꪪꔔpprllٳgkjjb< vww|ruu58_;˲hApD˻vz^{V#FX~=qӧ4+Hfn׮qC~Ddcc@Կzݕ|ي3f X)DpX.#^4U ˲&ݻwG) e2?Htss׿viP6 C]]]џӧOVTT؀-ƍggg'zX,6VVVFwmkk wddŋe2xOׯVݱcǴilll#"/_vss:t(}e꫟~k # N߭[Ν;[lD-6GK`4>|$$y1ZjFqqnj#$cctttϹAH$߿jj* յk׮~T*+++ׯ_߯_?F0Ç:3RjjjT*UuuJ wppعsÇ!9VVVֶO>&iҥǏjӦM6m C~ ;ھ}]FDDK8Sk˲}vvvAt)55lm7RmR[("FLs>x,i"l0@ Q'.}ce|6˲ҥKGC sȯL&suue?>|xHHHll,2 2 RYYYeeL&.# 8uĈݻ7l 5c7a ǎ+JJR$H';vh4IIIRffVСCXXؔ)S$ɚ5k\]]###Mv֭-[}۷/ޠh4LM <Z q@L #9!n2  "e++@x˲l߾}o>h \ ׯ6… ӦMK?q>#+@D>ӸBHf-9daRRR&O  ڵkQQQ1PRX5kVbb"WE"x=H弶f͚+Vd2[=QF9::r6v1c0 c4T kk.]XtAWX^4iFJ,|L8Q$]p,为ڴi.^`0͛7`0444׃7CCC;uzի>}ڹs!C 2T qK!a3 s…^{]իVVV7nܰa'|'i)M&Snnn.](!Z Ҕ"^" "F<#7`9!M.kkF.T*0' V8:{l׮]E"O?~z\ꕟ_=Ae{իM&ŋ'LPUU*RHKKVTT*R9eDgfGnZ\0͆ ܒ\`^z :tРA...K.8p`ƍGq8R$9RP+9|puuիWAI\]]={:5o!B.E/ )0666?X+,,e^z]~=88 , Bu 37W/0 ?߸qcڴiSNǍz믿3""G...jg}ֶm[%&<<!p+##㫯R_}UxxH$zUݗ-[f|uwe߿JJ,fs^^/\ׇ+"""$$gȑ#`Mt7???''Gq/(UV=~xܹO<{RiVV'x3fӧOu:hD"R c"Ʒ%DQA Y5666666x{_/ )S~&+V˖-ާ 7n *(((((,t{ܹ֭[cƌ1)));w_1o>}paÆ榦8q*,,ԩSo&iȐ!@`66O8{pOi/^d-F#VQ@Q"ȥ|o6}mݺu 8:e333XVVF|@=<|)4 'x U<>$|!Hp_J}Rv,YwO™bǎxbQm0***>}h삂Xjƌ 5W!'ϗ8lFCo[rurå'?-Z ED 3~MR(Jnm1 Hxw!..zm $~\E :tѣFj۶`޽{jjjJJJVVZرc.]ϟ߮];'''eׯ_|rd{\C`^/**:tЛogϞٳg#[Bo߾m*Yf$3Fمǔ2N+m!OSIx 9a=_dqR{Eaӌ1hͦH$:u/p̙۷t4[2,88xѢE۶mkhh(|@:utE #p/< wQ |o,k46mڔQƄ"DdDׅ  _~k׮9r$ (++S.Ϟ=z"$Qtդ$wwwJ%0^ c|B(q"Y489 3XxV ZXΝ;[lۈϯ7§>}|w;v4{r0DڶmM!ȏte`+jā(Bǯ}#=让Έ5|WH\oQJ8"VF(H,a4|] p> D"l5̢m ATx{{#+I#GrgS@8/ Ìaĉ`tWWWD/l%"D] @b(lH>1LL/r0OH*! &X6χ7%ܸqG^#r0Fؠbv'0 ^k(*p9x!CZQ%ĝ" i>=cW-X@H 'i쵡CGx@!6}.,pPz$8% W)|H>B2Z n_+PHI|q㑖QAdm@tr $Z.i|OU#Kt>Wp$x#Hqr~WNL;ti|D1ϳDP$S*6r.xBWX߼yÇDf<~'+1# 1QH1hRb3+`sns㨦OC,ĿϕZOjv^E"ٳg|%0|B!NM NM,-rnOSw޽rVYlO T2 prrrwwe$r a#֥KJUYY N<ŋ @"G9t&-B)ļB | G7R;S/:kD_$@<|%#s X:@./'DV > HEHTD`-8;\$!<N.m{q|Ha!)ى Qx ͒ZP,5.^^-ꢀpr,T>u9 6zBeKL8jeHkqt { H8M]fcxG‡tx@?qɠXka|@ܺf_bO4.|l >x_rOJ|B9F[T]BaVqE]~37s""X^@$?x~BZ"j7;( ~6)K`[L-"'RGקּ|ɕcؙ36/hь02䛈ҧtm6 q2Lo(XҌ"ںdE _HMlj[*-Nqi[\ {iV= I,(p՗bFsB ]HaQ)Č|qhqk+D;x/=QH—^@pPPjaM7ޢ(F0@D.!( |BN߉JRo w} |B^o2z>oDgk0 }NȲlAAA=ݻ>|믿VTw"AR aV*Z9@CBB0ՕsѩFR a6 YYY;v¬j;t aʔ)K. 83?)oV|||vv6W*Ӄ) ԰0uww![,D:={, @ @BaooϝL|gggn0:/۵kG6>rBZ>x`RRǓg!lٳgcbb_F#G֮]g "<99yΜ9HLL|aBrTRR2i$3fP8a"{X#0Yfeff%p 8fX/^|!fy޼yO<(ӧOSӲeJl΁?`Is qٝwעV!QS&.]QKђV<痽[B ltlIӸrwYuH".M`3.$O#H$/g7ntq[lyxŊH]/ /+HE`0u떑akk+z}@@۷uۀ~A޽qeܹSPP={zjeeebb"dBu=%%s휊GeeeFFFҽAg0tVVV7nptttvvH$~ A$D"ŗ/_0FY~}xx8̇E$길gggHRRٶm[faÆ}jkklmmrСC]]] o`mɒ%mڴK.//D'N}0 ÈD p9KRlll&O,xYӗq^c'K4 ۶m3 O#H1Lqqq;v8&oٲE[4L Vy @ٜ믿[4eل~b j‰ϥĿǎ"977ݻ`Nxnn{8 Pܻwo׮]B(,,wޞ={X$7@III;wr&&&ܹb3w<))iǎ i9]v544_zO>۹sg}}}UUU\\w~-Q$i4]VWW_pn^Ϟ=Kg6 wﮭ;u%$^gϞLl4sCCCll,n6ۧRZÇ钛JD>x ^jG]|YT޸qC'ŸT*t朜cǎTǏc($/͠ǏjkkG}zÃ\WWwqJ%zwߵ瓬bccKKK/>fR"J?~Eln:f̘,X"!;ΝdgsPPо}uUeY6((:t޽;Œ ڻwohhhpp0X"cJߐ={DDDp,qNvvӷo_"@?ų;::fee1 VgΜiccChooc43Z3wZ8^D"\./--h4ӧOCBd*JT644L6ȑL&.))1Lo񆃃T*h4yyyf7prr஖J:.;;e٩S+ɸI'947nPD{u E||@+Weҋ/ L^QQqYZJ̙3]Thӧd7gΜQ*;wB3˲ϟ///(]wʕ"<1!!@W^ nX^@b7m<v*jE _0X^Uho!@0z-\%T _K6}I/Ca4^6/%Ԓ/{ /D N@UUU"\ܿ?77W$!Jܥjwihh`YX޿????777==aXlD*N/))yZ$joj .)| Q㒇L B\___]]B_ډ "99aRaaj+++ZmEE|- h4j:55UV+J>DWC,0pDB6:Kc"/HTfee듒lllDzl@@@rr+RQQQUUSXXheeagg0 vAy CfffeeeEEΝ;/];88<|S$FDD8qƍݺuH$EEE:tppp8sLuuǏϟ?߽{k׮O7t:]iiiQQӧOoܸѮ];?%2[_X ,^&1%/...**)---,,w͞={D"JJ+T*+++sssDӧO#-- _YY ^ VVVo۶ѣG...JRR'OIҚwﺹedd+ǏfffvԩJ >>ݻ:.--ӧ&VVVwܱ3 iiiȖEHALGD(j˖-51cȑ#7o|-aϟȑN:ֺr\ v(rrr*..V(zT.͇H$JKK@m Wd"hѢER{ʲlũ6LN5k x}555 ^_WW䔑j ޽0 |Im tMBnݺ5''aӧ5ܹs7nd&00;x`.]jjj\]]A222ݓmϏa;wxyy%%%߈D#G!!!˗/dVVV?L&xxx80''d2kN,={W_ tvvoWUUچo ڴiSSSx )&&\ܿ ,),;55U$0 뛛o2BCC&eZ6++ Aս{={QN^t)cll,bhhz}~~~uuZm=|ʪ}YYY:us0D*|ĘmeeV\ .߿߱cǽ{e}}}#""eeef8^R EUUZ DDEEEEE1 SUUu6m|7 `RWW^4{ə3g|||233juuuuΝZmnnnXXL&K$[[`(D"i׮]jjꫯZ\\\=(-- LZ~H$߿D"3='!o}}drttl1;c:*++kӦMSgddxzzM&eoE ݤz,pVJ^Uz%s—^jvE?@,[ц!vWD-.q8I$~q"> endobj 342 0 obj << /Length 15108 >> stream q 15.000 35.584 577.500 741.416 re W n 0.965 0.965 0.965 rg 26.250 700.968 555.000 76.032 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 700.968 m 581.250 700.968 l 581.250 701.718 l 26.250 701.718 l f q 35.250 712.218 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 5: Summarized data from mixed FR5/PR training across sessions in Experiment 2A \(left panels\) and )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 2B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the initial FR5 training period are presented in the upper panels, while data from the PR training period are )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(presented in the lower panels.)] TJ ET Q BT 26.250 683.944 Td /F1 9.8 Tf [(Analysis of the FR5 response rate as a function of the number of reinforcers earned \(for the initial 15 reinforcers\) showed that )] TJ ET BT 26.250 672.039 Td /F1 9.8 Tf [(the z_Q175 het mice responded consistently less than WT mice \(Genotype main effect: F\(1,7\) = 7.68, )] TJ ET BT 467.096 672.039 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 508.027 672.039 Td /F1 9.8 Tf [(Figure 6 upper )] TJ ET BT 26.250 660.135 Td /F1 9.8 Tf [(left panel)] TJ ET BT 65.816 660.135 Td /F5 9.8 Tf [(\))] TJ ET BT 69.062 660.135 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 640.730 Td /F1 9.8 Tf [(The inverse U-shaped response rate curve in the PR component \(truncated at the 120-response requirement\) suggested that )] TJ ET BT 26.250 628.825 Td /F1 9.8 Tf [(z_Q175 het mice responded slightly less than WT mice although this difference did not reach significance \(Genotype main )] TJ ET BT 26.250 616.920 Td /F1 9.8 Tf [(effect: F\(1,7\) = 3.74, )] TJ ET BT 117.032 616.920 Td /F5 9.8 Tf [(p < 0.10; )] TJ ET BT 157.963 616.920 Td /F1 9.8 Tf [(Response Requirement: F\(10,62\) =5.11, )] TJ ET BT 335.442 616.920 Td /F5 9.8 Tf [(p < 0.001;)] TJ ET BT 379.083 616.920 Td /F1 9.8 Tf [( Figure 6 bottom left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 332.021 555.000 275.018 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 607.039 m 581.250 607.039 l 581.250 606.289 l 26.250 606.289 l f 26.250 332.021 m 581.250 332.021 l 581.250 332.771 l 26.250 332.771 l f q 225.000 0 0 169.500 35.250 427.789 cm /I44 Do Q q 35.250 343.271 537.000 78.518 re W n 0.271 0.267 0.267 rg BT 35.250 412.266 Td /F4 9.8 Tf [(Fig. 6: Collapsed response rate data from the 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 400.361 Td /F4 9.8 Tf [(Experiment 2A \(left panels\) and Experiment 2B \(right panels\))] TJ ET BT 35.250 380.991 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 367.255 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 353.518 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 297.800 Td /F4 12.0 Tf [(Experiment 2B)] TJ ET BT 26.250 277.845 Td /F1 9.8 Tf [(As response rates in Experiment 2A were low overall regardless of genotype, likely due to the advanced age of the mice, a )] TJ ET BT 26.250 265.941 Td /F1 9.8 Tf [(second group of z_Q175 KI mice were investigated at an earlier timepoint, to establish if a more robust phenotype might be )] TJ ET BT 26.250 254.036 Td /F1 9.8 Tf [(detected at this age.)] TJ ET BT 26.250 234.631 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 215.226 Td /F1 9.8 Tf [(A mixed sex cohort of het and WT knock-in mice, 11 per genotype, was bred in our facility by crossing z_Q175 \(CHDI-15-1\) het )] TJ ET BT 26.250 203.322 Td /F1 9.8 Tf [(male animals with WT females, all on a congenic C57Bl/6J background. The mice were housed and maintained as described for )] TJ ET BT 26.250 191.417 Td /F1 9.8 Tf [(Experiment 2A, above. Prior to this testing, these animals had been evaluated in the PhenoCube, a comprehensive behavioral )] TJ ET BT 26.250 179.512 Td /F1 9.8 Tf [(screening platform, and the tapered beam motor assay, but were nave to operant boxes and had never before been food )] TJ ET BT 26.250 167.607 Td /F1 9.8 Tf [(restricted. The mean CAG repeat length in the z_Q175 het mice in this experiment was 189 repeats, with the cohort ranging )] TJ ET BT 26.250 155.703 Td /F1 9.8 Tf [(from 184 to 201 repeats. One female z_Q175 het animal became sick during the final phase of testing \(FR5/PR testing\) and )] TJ ET BT 26.250 143.798 Td /F1 9.8 Tf [(was unable to continue. As a precaution, all data from this animal was excluded from the dataset analyzed. These animals were )] TJ ET BT 26.250 131.893 Td /F1 9.8 Tf [(27 weeks old 1 week on the initial day of instrumental training and 30 1 weeks old on the initial day of mixed FR5/PR )] TJ ET BT 26.250 119.988 Td /F1 9.8 Tf [(training.)] TJ ET BT 26.250 100.584 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET Q q 15.000 35.584 577.500 741.416 re W n 0.965 0.965 0.965 rg 26.250 700.968 555.000 76.032 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 700.968 m 581.250 700.968 l 581.250 701.718 l 26.250 701.718 l f q 35.250 712.218 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 5: Summarized data from mixed FR5/PR training across sessions in Experiment 2A \(left panels\) and )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 2B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the initial FR5 training period are presented in the upper panels, while data from the PR training period are )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(presented in the lower panels.)] TJ ET Q BT 26.250 683.944 Td /F1 9.8 Tf [(Analysis of the FR5 response rate as a function of the number of reinforcers earned \(for the initial 15 reinforcers\) showed that )] TJ ET BT 26.250 672.039 Td /F1 9.8 Tf [(the z_Q175 het mice responded consistently less than WT mice \(Genotype main effect: F\(1,7\) = 7.68, )] TJ ET BT 467.096 672.039 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 508.027 672.039 Td /F1 9.8 Tf [(Figure 6 upper )] TJ ET BT 26.250 660.135 Td /F1 9.8 Tf [(left panel)] TJ ET BT 65.816 660.135 Td /F5 9.8 Tf [(\))] TJ ET BT 69.062 660.135 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 640.730 Td /F1 9.8 Tf [(The inverse U-shaped response rate curve in the PR component \(truncated at the 120-response requirement\) suggested that )] TJ ET BT 26.250 628.825 Td /F1 9.8 Tf [(z_Q175 het mice responded slightly less than WT mice although this difference did not reach significance \(Genotype main )] TJ ET BT 26.250 616.920 Td /F1 9.8 Tf [(effect: F\(1,7\) = 3.74, )] TJ ET BT 117.032 616.920 Td /F5 9.8 Tf [(p < 0.10; )] TJ ET BT 157.963 616.920 Td /F1 9.8 Tf [(Response Requirement: F\(10,62\) =5.11, )] TJ ET BT 335.442 616.920 Td /F5 9.8 Tf [(p < 0.001;)] TJ ET BT 379.083 616.920 Td /F1 9.8 Tf [( Figure 6 bottom left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 332.021 555.000 275.018 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 607.039 m 581.250 607.039 l 581.250 606.289 l 26.250 606.289 l f 26.250 332.021 m 581.250 332.021 l 581.250 332.771 l 26.250 332.771 l f q 225.000 0 0 169.500 35.250 427.789 cm /I46 Do Q q 35.250 343.271 537.000 78.518 re W n 0.271 0.267 0.267 rg BT 35.250 412.266 Td /F4 9.8 Tf [(Fig. 6: Collapsed response rate data from the 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 400.361 Td /F4 9.8 Tf [(Experiment 2A \(left panels\) and Experiment 2B \(right panels\))] TJ ET BT 35.250 380.991 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 367.255 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 353.518 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 297.800 Td /F4 12.0 Tf [(Experiment 2B)] TJ ET BT 26.250 277.845 Td /F1 9.8 Tf [(As response rates in Experiment 2A were low overall regardless of genotype, likely due to the advanced age of the mice, a )] TJ ET BT 26.250 265.941 Td /F1 9.8 Tf [(second group of z_Q175 KI mice were investigated at an earlier timepoint, to establish if a more robust phenotype might be )] TJ ET BT 26.250 254.036 Td /F1 9.8 Tf [(detected at this age.)] TJ ET BT 26.250 234.631 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 215.226 Td /F1 9.8 Tf [(A mixed sex cohort of het and WT knock-in mice, 11 per genotype, was bred in our facility by crossing z_Q175 \(CHDI-15-1\) het )] TJ ET BT 26.250 203.322 Td /F1 9.8 Tf [(male animals with WT females, all on a congenic C57Bl/6J background. The mice were housed and maintained as described for )] TJ ET BT 26.250 191.417 Td /F1 9.8 Tf [(Experiment 2A, above. Prior to this testing, these animals had been evaluated in the PhenoCube, a comprehensive behavioral )] TJ ET BT 26.250 179.512 Td /F1 9.8 Tf [(screening platform, and the tapered beam motor assay, but were nave to operant boxes and had never before been food )] TJ ET BT 26.250 167.607 Td /F1 9.8 Tf [(restricted. The mean CAG repeat length in the z_Q175 het mice in this experiment was 189 repeats, with the cohort ranging )] TJ ET BT 26.250 155.703 Td /F1 9.8 Tf [(from 184 to 201 repeats. One female z_Q175 het animal became sick during the final phase of testing \(FR5/PR testing\) and )] TJ ET BT 26.250 143.798 Td /F1 9.8 Tf [(was unable to continue. As a precaution, all data from this animal was excluded from the dataset analyzed. These animals were )] TJ ET BT 26.250 131.893 Td /F1 9.8 Tf [(27 weeks old 1 week on the initial day of instrumental training and 30 1 weeks old on the initial day of mixed FR5/PR )] TJ ET BT 26.250 119.988 Td /F1 9.8 Tf [(training.)] TJ ET BT 26.250 100.584 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET Q q 15.000 35.584 577.500 741.416 re W n 0.965 0.965 0.965 rg 26.250 700.968 555.000 76.032 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 700.968 m 581.250 700.968 l 581.250 701.718 l 26.250 701.718 l f q 35.250 712.218 537.000 64.782 re W n 0.271 0.267 0.267 rg BT 35.250 767.476 Td /F4 9.8 Tf [(Fig. 5: Summarized data from mixed FR5/PR training across sessions in Experiment 2A \(left panels\) and )] TJ ET BT 35.250 755.571 Td /F4 9.8 Tf [(Experiment 2B \(right panels\))] TJ ET BT 35.250 736.201 Td /F1 9.8 Tf [(Data from the initial FR5 training period are presented in the upper panels, while data from the PR training period are )] TJ ET BT 35.250 722.465 Td /F1 9.8 Tf [(presented in the lower panels.)] TJ ET Q BT 26.250 683.944 Td /F1 9.8 Tf [(Analysis of the FR5 response rate as a function of the number of reinforcers earned \(for the initial 15 reinforcers\) showed that )] TJ ET BT 26.250 672.039 Td /F1 9.8 Tf [(the z_Q175 het mice responded consistently less than WT mice \(Genotype main effect: F\(1,7\) = 7.68, )] TJ ET BT 467.096 672.039 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 508.027 672.039 Td /F1 9.8 Tf [(Figure 6 upper )] TJ ET BT 26.250 660.135 Td /F1 9.8 Tf [(left panel)] TJ ET BT 65.816 660.135 Td /F5 9.8 Tf [(\))] TJ ET BT 69.062 660.135 Td /F1 9.8 Tf [(.)] TJ ET BT 26.250 640.730 Td /F1 9.8 Tf [(The inverse U-shaped response rate curve in the PR component \(truncated at the 120-response requirement\) suggested that )] TJ ET BT 26.250 628.825 Td /F1 9.8 Tf [(z_Q175 het mice responded slightly less than WT mice although this difference did not reach significance \(Genotype main )] TJ ET BT 26.250 616.920 Td /F1 9.8 Tf [(effect: F\(1,7\) = 3.74, )] TJ ET BT 117.032 616.920 Td /F5 9.8 Tf [(p < 0.10; )] TJ ET BT 157.963 616.920 Td /F1 9.8 Tf [(Response Requirement: F\(10,62\) =5.11, )] TJ ET BT 335.442 616.920 Td /F5 9.8 Tf [(p < 0.001;)] TJ ET BT 379.083 616.920 Td /F1 9.8 Tf [( Figure 6 bottom left panel\).)] TJ ET 0.965 0.965 0.965 rg 26.250 332.021 555.000 275.018 re f 0.267 0.267 0.267 rg 0.267 0.267 0.267 RG 26.250 607.039 m 581.250 607.039 l 581.250 606.289 l 26.250 606.289 l f 26.250 332.021 m 581.250 332.021 l 581.250 332.771 l 26.250 332.771 l f q 225.000 0 0 169.500 35.250 427.789 cm /I48 Do Q q 35.250 343.271 537.000 78.518 re W n 0.271 0.267 0.267 rg BT 35.250 412.266 Td /F4 9.8 Tf [(Fig. 6: Collapsed response rate data from the 8 sessions of FR5/PR training broken down by reinforcer for )] TJ ET BT 35.250 400.361 Td /F4 9.8 Tf [(Experiment 2A \(left panels\) and Experiment 2B \(right panels\))] TJ ET BT 35.250 380.991 Td /F1 9.8 Tf [(Data from the FR5 training period \(upper panels\) are labeled per reinforcer earned, while those from the PR training period )] TJ ET BT 35.250 367.255 Td /F1 9.8 Tf [(\(lower panels\) are instead labeled by the ratio requirement, such that each datapoint indicates the mean response rate )] TJ ET BT 35.250 353.518 Td /F1 9.8 Tf [(while working on that ratio.)] TJ ET Q BT 26.250 297.800 Td /F4 12.0 Tf [(Experiment 2B)] TJ ET BT 26.250 277.845 Td /F1 9.8 Tf [(As response rates in Experiment 2A were low overall regardless of genotype, likely due to the advanced age of the mice, a )] TJ ET BT 26.250 265.941 Td /F1 9.8 Tf [(second group of z_Q175 KI mice were investigated at an earlier timepoint, to establish if a more robust phenotype might be )] TJ ET BT 26.250 254.036 Td /F1 9.8 Tf [(detected at this age.)] TJ ET BT 26.250 234.631 Td /F5 9.8 Tf [(Subjects)] TJ ET BT 26.250 215.226 Td /F1 9.8 Tf [(A mixed sex cohort of het and WT knock-in mice, 11 per genotype, was bred in our facility by crossing z_Q175 \(CHDI-15-1\) het )] TJ ET BT 26.250 203.322 Td /F1 9.8 Tf [(male animals with WT females, all on a congenic C57Bl/6J background. The mice were housed and maintained as described for )] TJ ET BT 26.250 191.417 Td /F1 9.8 Tf [(Experiment 2A, above. Prior to this testing, these animals had been evaluated in the PhenoCube, a comprehensive behavioral )] TJ ET BT 26.250 179.512 Td /F1 9.8 Tf [(screening platform, and the tapered beam motor assay, but were nave to operant boxes and had never before been food )] TJ ET BT 26.250 167.607 Td /F1 9.8 Tf [(restricted. The mean CAG repeat length in the z_Q175 het mice in this experiment was 189 repeats, with the cohort ranging )] TJ ET BT 26.250 155.703 Td /F1 9.8 Tf [(from 184 to 201 repeats. One female z_Q175 het animal became sick during the final phase of testing \(FR5/PR testing\) and )] TJ ET BT 26.250 143.798 Td /F1 9.8 Tf [(was unable to continue. As a precaution, all data from this animal was excluded from the dataset analyzed. These animals were )] TJ ET BT 26.250 131.893 Td /F1 9.8 Tf [(27 weeks old 1 week on the initial day of instrumental training and 30 1 weeks old on the initial day of mixed FR5/PR )] TJ ET BT 26.250 119.988 Td /F1 9.8 Tf [(training.)] TJ ET BT 26.250 100.584 Td /F5 9.8 Tf [(Equipment and procedures)] TJ ET Q q 225.000 0 0 169.500 35.250 427.789 cm /I50 Do Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(7)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 343 0 obj << /Type /Annot /Subtype /Link /A 344 0 R /Border [0 0 0] /H /I /Rect [ 35.2500 427.7895 260.2500 597.2895 ] >> endobj 344 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 345 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 346 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 345 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 348 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 349 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 350 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 349 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 352 0 obj << /Type /Action /S /URI /URI (https://currents.plos.org/hd/files/2012/04/figure3-1.png) >> endobj 353 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 354 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 353 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 481>> stream xA 0 =fy9뀟\Aի endstream endobj 356 0 obj << /Type /XObject /Subtype /Image /Width 300 /Height 226 /SMask 355 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 300 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 47981>> stream x}g\o0%G Ī9`5!VxwuYY]u ꪬWQ$AE@$DrNCzC]f@=WS}?aEQ  QB a OONh !LЅ72 Y,HB@O' @ddJ "Yx\D, 3$3P0YHIł"_fCBNW>Fd27:RÐD0PC qI$J)kZ,(nc4HH|BvS2x?h_____W^tZO#O<_U."",O<֭[ \ZZMiRtt4>ep3220;v ϝ;' B!{5@+ .Y3}W@ٳg)ybd+,,mݺ'8>} B0''FEEcbbb]oFFv~r ۷oH$̤pDOLL GaX\YY)Hjkk@ 9D")///++p8---_kkk߽{ᑑtedd,_<((=vÇkjj@̼&SPP {zzC]] T"WTT$UUUeeeH$UUUB痗766555 @۷o[ZZ@Dr@SNw۷osb,y(&&,66[[[R[[RVVzᲲr]RR@ xÇ{%߿$??`}}?ԄT^^^RRp1]]]|D[RRm6<++}H$jnn 555_ |>H$eee %H_"Hzzz|}}% (9rrr;r#baQQfKK7o`˻y󦭭*4A:;; v5--˗/O0[UU5<<-88>..,K]]Ν;\.ҥKB0 @AA!((hҥg[[ۃ'FLurrjhh/bG:mڴr333`^xxϟ?r8;;khhxꆆ`d^^D";wH$uuu.^hggn޼iaaݻ[n9::;oE$22r/^xquuH$zɌ3 A{{ɭ[jkk?իׯ_|####BAojjZTT\PP`nnpXXXYYو#'Ͽqㆾ~]]|>rrrܤTTTp8r]fggwܹ%K,_\SS3((%;; ˗/wvv744\z㥧CTRR2gUUUP8eʔW^-Yӧ***`33[,\000;11QSS399YGG'44tȑӧO?tźu떫@ x葏 Ås500rwr=5jԥK$ӧ%IbbիWϟ?/ǎx}0 _vmŊW^566*//-oqqqgg3g ˝¦O2Xuuurrr REEĉν^,޽СCǏ755۱c… ߿2&11QCCc֭ _~c[ r܆'Oرcܹ .LMMYILLlii X, u+W 8sիW!211y5$z&`+V }}}*** RWW̙3d\OOvpp͛!ڱcǁ=zիX'M988lݺj͛7o~^~ &ׯ?Q^^>yI&0aꪯr &lٲEWW믿^f Aw߭^˫ ###+++>>gܸqm#G4770aFUU&UVܹ/wUWWdɒ;v >X[__ p__[?nj;wrrrɓ'E"QEE Pe555D+Wxb qqqqde˖YZZpiimQQŋڄBass1cƍwر6A zY,!4iɓ'P7ٳg !{%Iqqakk Z[[Z";!xٳg7c\nSSWRR̙šK.ݹs9s̙chh*X9277wԩSNK,31Ĉ FGGAjjjSLqvvNMM>}.Yd̘1鍍#dP(#`!윝=o޼y޹sG$K<&'<<|{! ǘ|R p8 XXX`~ hܹӧO?233ǎ_ B Pؐ ufff(@%SBr _fCp¦~-!!b:th֛7oݻwAJJJjjj(:88(jkkf 3Æ \ti``?xQFA4|˗/GDD_gbbð=!;;{…cǎ}}}|A9s.\zjUUUEgϞﯧgccceelkkkyyym۶_U ؠ(/P544Ɯ SWW}٪UBQ}ٳ[t[^XөO2fWTTBlٲ+W޻wǏݻWCC[f>la˖- :88XXXՙ񪩩=ZZZ>>>uuu555ӦM(Yidd BIIbiiwɓ'8pTWW ŋ8qBOOoԨQ zzzPTT9rdjj?---;;;IA{ 6mAJؙGQ(&)#񔜜 ?(僦TFS"-@fǏdzA̍7(3ŋΘkb ~_eHǀǂϻH~ Q-**_= b8Ν;w ccc9=%tn> 0"A ] $LZf bwޝl2{{{rJ0䣴) .]*R't*VGd KG1B,s8o} N&6j(333B$0LZ8q"D~$}4×>̟?#//?l0:~mRgĈ2/$A cƌ!Dk3QB)"ӫKf~fD) [ H>}<@E;;;lvgg%,~ft+j>E.3/7W\.`kkWc}^orrrll@ IDHBI< )(BZ)L2Aɘ¬>OټPF yG.RQJ&^_&/aᘘuuu@0vgϞGׯ_߱cƼl2gggIFDD={AQڵkϟCQTGG,z@DkS˜ƚ|ZԈ{w'CK KkbO2999...ᑐ@J%Lb0<F):mLZbӕZZZ5 6\pRMLL֥K;̙cii 6 OG\5< HZ" 6m4eʔ@v!{yds@bT0<3P#?wDtit@_tREg͚Ze˖a[EEEj=^J:) BO6و#0?S,mǫRl gC`3t~ @SU^20]v vX AP~~~QQ@ UTT|P(NOOK󱱱gΜp8%EpMkk+x50 A4C1f~YFPpB~3 D)\Z}O|Eg% t)a*6  D`Ka8;;w?s|||nnn\\ڵk۠ӧ^~~: ...FFFJJJ0 _xQ,tK$}A'On޼:::g޹sadd]]]!9rdkk+͆aƦJNNNB3{oHɳ oݺ秭͌KCHx)9T| bg*C*- ,44?...;;Aгgrss{{{\=࠮^\\lkk),,rPeD< #%KMNMA߯;X/^;o߾n:ϯ111gM~R:t%MMͫW|.[PPrJ55n777SSS_677!(f6 gΜ #G6m ؇LHH\`6>r-,,@'L Lޓ?8q'TF@斟?~)S`p2mϞ=/_-++^tiQQ,+''gAAA~TTTrrڵk,رc˷At֭?SEEEEE޽{zzzgϞmmm=={6Aw2p_?*((S$MB+%-J!h|~[[[YYFKK zL H)S&DK$2iy 1!&?[ ~̙3jԨaÆΚ5 X`jj:vXP )5Mdɒ 6455|LMMBCC[ZZʪ{CPdΜ9SQQ_ XYY555(߿W^kii[d޶m'GQtٲe\.WWW{{{n2Ip 89}a f6y~apQG8;;Z̙3͵jjj|>?((P(ZXX|0(_p҆ LLL(NVl%?!-!8EFF7;B8}t{zzVZ5۷o7GKedhӦMt8 ;;; MNNnĈ9R555 ʌ/ea!'nlA***...(ZZZ9sCA<=}NQQqiiiyyy[l?~Ν;afځDUUU</&&Hkllܽ{  `6,pOVVVQQ 7N$L&]]]'L@-][[\\[[yCݻOUUUƎ AP@@˭~:s˖-ʺwb @߇ E{577"'%L% aڵ_pn>Ն @M0AYYYUU\cnn^QQajj l6fo߾}ٲe0Ϛ5ɓ'.] (##{wCۃۓb#G233⋤$0={.m %˃#'Pmjj =zE޽{W]]niiz5kրUPUUUH$&L@Ql۶mo?߽{t;ҥK`-ܹ3nܸm۶%$$kkkoذ\YYVOOO[[{(-[ EQmgg>\YYy…B?[th.< ͛7%566Ν;wȑof`b&===<())Z| /0{Ҳ6mڤ8g999PAWt HSSΝ;[nURR>tЦM 233o]ckkk=z3f`&'''} w0}vMMMuuuuuuB CAF79ा%ðvǎ333?(((pBYYŋKJJZZZBBB|}}WZUYY nXkkk;qΩ&3%%ݝ[ZZ0\VVWN֭#Pmiiiii6l\D ϟOLL5k+~!aPX\\ammf'MwƎ`:׽zjǎcS:uҥK 9NCCØ1c^x1|p'ǠΝ A~05ϕ+WFFF:uܹs֭(..>}4Z|y[[ܹsw5;v,hcc*۷oA%'JJJ` XwΝ3gΨ|ܛƾ~Ԕx"%%e͆|>ƍ <O]]4"555SN%TKEp__͛7Y,oY,JVV{{{Y,ݻwn$$$`UV_t|ѢEw$Q7n\||ƍQUWWWSSZ|ycccyy1cn߾^xq֬YǏ 0۷J2Q"ן:uÇOLL־~)`KY,BNMM:@P\\0rHwb._ *!CI{–08ydFF=2r2Ax={QMNNNNN/s?{,'''O,'' 6?V^fLf97i$E|cpݖ-[k׮{ϟ~ p&Hgdd4qĨ~ggӧ3g΀.Dر>//O TWWԠ(/( ^G\xB[[ u%J"ʜwu@r{MD"vƍ@\BBŋ񷭡ۦH|pË/ _5 ::E`Id/_'jjj+W$͛7ٳgX,F~8qbss3?'N}}}aaaD l۶ڵk$))iڵ/aXYY 1cbbb6lްaCCCD" 6mZii)h]UUnǂaӳŋNNN۷o~7|sرǏgff޸qܺ 䤦/'O[n  rϞ=6m%*$$ɓ'III===>>>W\? LW_"A(G_ d"}}}+VprrZ|9iooWSSSUU3f ~PNNe\+ehɒ%uRSS-ZDH^RRRSSTTTpB___>…ٳg_|Y^^x_jUIIIooZCCC[[>APbb"XܵkWkkD"ы/QwP `EtѢEWܻwXg/ n4])@n݊e(ڶcx{{XtÇE"QMMMDDě7oYYYI$ٹkرÇp8ˣ(  /600غuu W+444ql6x,+##\Aٳ/ӧX\hMM IMM544 innVQQ9y$uժU󫫫;~8 ה)S:$._|~ xXϟO6-++둑)))/^666F+>>>##l}3gUCBooo#,W0dsssp Z1 ;c^|%'''!Fzj!kkkkcc3}'NDM6[nƍ7n5k222@;w\PPWX!X,֦M-(ax~MjjjGGX,]/ϝ;w'bVD"ǣvqqILLtԄ {j|$e}}}rrrW^U}رYfM2\:,֬Y#y```  ãG{۷ܹݽeeee!!!OJJq!  ===eee`!"|+{6-v͞=[EE\+H[XhĉMMM۶m ݺu+ Ï=ܿ @xbll,xuɓ'/^|𡱱gXpٲe!!!SNݰaѣGY,[[[z޽{!,..fh`ի7n ^b <Bnnng`` K(f͚'Ooذ۷o055o/^rJ999m6,--{<""bs?~<5jTZZ+W>??͚5MCQTGG'00Ӝ[ZZl2++2{{B_Ο?M(&$$\xڕ+Wrss/^sN;;s綶 B vrr3fҥK,--%|>)+++**ڵU7oyk.\ޞvܹ7onڴ655ݲeKPPPcc۷>LPb@= ſBȶ_I˘ Rp. HhII 8//7o&&&.^ɓÆ 5jgg+lmm5k/ĉ_lo@@+WLLLݻ8<}4%%e֬YF3aGs-6lؐs) Ikll gllL*Lg͚emmmaaAФI6n*)SXb߿khh`a|*))M4 A****++g׮]?ƍ׬Ys/EQGGGHfWZ7466Ι3lEl'N8b999//~iڵ񇲲[322,X[]]h@@6)++ٳG]][GGGYYy6mX~=}H$:p̙3===---;::V^ե%@I^^^CCzF^  8s F.5%̠P,3DEEm޼رcׯ_GQÇaaa zEQlub822rׯ_oܚO:hlllEEp8gL4(fff ߿kΜ9|>|l|Q*EEEn"Oӏ9ᅢ+* ]]ssUVq77P󋏏'ݶmX|~aa!A&@p8^^^wAQ… (DK.Q:uJ,߼yhӦM(;vEK.L-]իWO{._(644477˿'ObyDbի@8ǻ&%%]zmʔ)iii`AL=!URR5 999D~Q%Z4Tڕ؄G BDbmm__vKB׎r۷oݺu%/^\__hѢ]v}7ؒYΝ;'핔!'N믿?jkkdf#5r}-\./_̾Vтv.̞޼y(''bׅ|$'Yre{{;p:u[33'Oɓ'əU$x(**5 zBȑ#JJJÆ sp8pF\RRx[;22l9rdcǎ>|pYY_r߿/fΜbcc'VX~999ȼjժΟ?߾}w%(`EEv]] xBIInԇ~8EpK@X$eUCa@+<^иb 6-//ϼ hѢJb455ك-[AF{NMMm˖-@ F޿۩S|޼y[llllΝy+W߿bΝ~~~ӦM?~<-999!!;ɮ(z{{jjjzzz\J'g<0L~---Qmoo&VVV='l6_]r%A,lլ[D" Հ%7n#=8||O^^lx}(f(;:::o>ʰj\jLٵҹK8ϟwww`^CCcذarrrӧO 244 hѢEdJJJb O?_pٳg;&''WZZݭ VaT#G믔0p̙3g|?Յ ӧO v쳍ׯyJd,f_$Y'tTpy ǏaPTVV^xUUUKKK֟ @`kkA/bRJ_miiyɓښXt0A&yٳL1󝝝:;wN _^nAYYZB!`/m۳g"8chhH'V!?~XUU|aggg e2()K0K`(UjEEEҲX,CCCfGi=p b[{OGf|)Szw[ N:u'Ǐ _llljj;V`PFF۷o[ZZ _xmsIb Ɐ#?s2PdDI( xOٹs'D㉺}2eJnn)e?N i$++ݻwcSdTR;f?~|EEikkG6779;;KqqTxWW׆cʒ_C"YF=R1TedI; h``̙3 C[?1Og.**111555zAВ%K/.]:lذtϔΜ93|p333{jCݼy3#E^Kħ%3CIǃaN#>`X!ߏJvNIIIsssww7AG*:::55ܗO2u`'\xN={vWWe0@|],@2BBY RJH)V S '''!;Ekkk333/^7uT0FŌ\ :*|޼y?-- TVV޸q7?ijjbS:MPPP011iiiOPUUU9s&a.bfPs >2 ,dHܬ͍ A}}},K"`[ \u _Kj#/P)#{=&c͞=[ lmm8&U;eD… 0 ܾ}NWEv255޶Q G! B2/4 ' y#ѐrOUUվ>pZ/?|3)x1$@U̓c:Y;)[E  *++w|<$6ڧH,JdsJ :lJl6 ' >\`AqqX,^r倆s4ڂuIYV2eBF0 %%3C&djjjhQQQQQͶ!\Ȑ5Rbrd 9 !d1kdd$//577O:;?K7Ň`Inj2HLVD&#@rddkk .kbbbaa>Kj*pdfC .9̓kAfRRRW^ ,--  \ECJ)F l19o޼HLLDQT"ܸqCQQQ__ӧ#Gkr\wwwDr^$eKNrB9A)9g|{{{ee PTT4cƌϘ1CAi°!G7 ɒ 1ց~@^a*\-ɐ(߳gόfΜ ujEAm`33@=H)9-9B𠶶d)KCC\pBBB`` YypK2#sٹtu@nJ.0&>GHeo?|Qѱb B СCYYY  `"D5AƂ !J p˖-:::I 7yׯ_qƁ!; !$,_[;,9-]7?Jɢ(RSb*!muuJ <A,777> NT:R#@G F )BBB}*x#GJ$p8 tD9#<~: gl@2, SRR jmmž"MMME"%`stt֦yʘ.-'+%WHm'TQQƍ{ŗ_~ A#'J|kDh@B^n*3vtt dSܧ#U<2Z.#}[P ,K͖I_&L0a} "lG{D1td?T=(f,yHh@gH dR> ceEhF :ʩ- Y ,Z98MFc(2;PL12 'Hcv,b̲H 3P(,--H$YYY;eGH`&'kä߿ o!n,R꜊` i.- k;:::::RSSw* )[>/YB+4('DvttyFCC,((m"nJRdB)C5Fwr \>dȜ ~8pSRRrrr@|ooP(g9g(Gb@) R%bH6l@ʴdHrywRD( Z 3r777|[[ی _~n\\е#$t*bdL7,4Yb1TUU{nܸq999K[0 [N ],_˞wT!ߤTWF}9h4A 4ɢk~ND*9nrK&2:5' x(R@چ1 7 vL: 1R2PzLjѢcD/:]O&H/^^<Ön֋bh EaO@Wp)'LCc0STP2ċH .HghRel;lu!]K KB.Ax]hhhcc#L>|+o( CFr$t "$O<%k' $1O>MJJH$t(ӧHCR, WHʖ s ^JrrrÆ .wd6HjBs \RJc-BR.RHY444VSS ?hooCe!Ks**gΜ >dAoA~~RoP$S  9!C3b@ ?xI}b,G2?YU Pre8eO[ I! Ȯe'dHFGtt*b~lvAFC~P l!K cL[QQq.//w o߾}5 =B$&&,--),,| IIIW~~'O@9A#F@ҥK |eGGGACBBihh G.<<oܸa|Ѳeˤ@@\./_X,A.\0 AAA!ry###E9{ fffAӧO[XXbAN:emm- HP -H~:A b c ***# W\QUUekhh!r%---'b1cTTT{{ <www#r]. ȝ;wx<* n***ܼy###E"a 1bp;EId&HЀקp]]]zz:ԼxEQDR]]⫪233A˗ \QQ \쌏ᎎǏ %HZ[[Aɓ' Snjjz766pCC1\iii \[[a{xpooG@FGGpOOOLL wwwƂpWWW\\%F ILL|4H$b⺺@866cbbzzz@8::Gz{{AÇ< 33̬gSRׯ_?xtDb1 ir94;MMLƹHpTJ/hrr)&&LP(]=KK~zUU~('M|` c͛7KJJt{]޻CwaJSSSmmmDDrrr8f8찰L&{S|CffD"sP(޽KJXTTtرt\T@VVVtt;wp-(zQXXXll,.< 99Y(^~GPĐ(--=uTbb;_4{޹sΝ;HٳgW\!폻1.!#x@Oa믿N>ގKJJjmm=x`kk+=::Z,޽/<==O> 'OlllnݺE*C544WWWLחzZnccg ~]]ݳgcmm>%00VWFnYԆSί^ Bȕnݚ7oX,Bȕׯ_= 0aBppH$Bkkk–/_(lɅ#GT,C:877ή}Ĉ0~zCٳ'$.Hϟ2tPx\.ֆsrrƍ( 55[[[@ּAP ڵk[TZҠ(jdRkkk_UUUV˗/G YbEYYj*2nŊ7m[[۱cZZZHVN{! CjjݻI:YYYlIĉpL?u~SN) ZӧϞ=K9sرctBQ\\|RNxJ+<DAŴtRw#)udħ(ve'S(pų FzCCއ# ɰ񃤷 ޗDpsӧbZUUeaa,--?T2S,Ӯ*߉5RP0?~?_KKKUU.../_OKK {RLKKkll ޿PPѣϜ9gVUU褥⊊ ]]ݶ6ʪ}ʔ)m ]-?&@'755UWW3: --MCC#88޽{4'8p`ĈȉE 0ZGG'55Ãщ'Ooi#i 4dnDA;::ܜ!DRJ[[ڪkkkՕ}/^Jiii \[[MSSS&yxx 6HA@dAfNlhhps"Z@N D$˗/r۷o-,,𵦦T*𰷷pK 2>|> y,ha!mX2 NӨ:-;88dff:99=x۷<޾`oo ;88^^zQ[D*Dbnn.---ccc $J@S r)YY<[T?8;::;;;?xwωp͉:::8MAZf4x mBQ^TZs\A!?Bv~[F|;zTei[n*ɮnU*0'HbccRirrrxx8xFnw@fffmm-%<<<%%?#nܸ13\ʸ2]UyFC6.D"͕H$(KFF\r]VVիlRg{&EQ0RP(8_!'eee***H' -+̗_hճGGG'''lllƏN8ŋ>аojjJplGGwNNׯy6+G@@ݻwy[[[ W^⠠Ҿ}fff^zU ߿4??.ӅREDDz PXXxĉB7-X:PRRRRR0`@QQrbzz:tqz7Ot(Œ`nnޯ_?trrRSSSWWٳs>쳛7o%J KKvPWrVUUD"z҄[bdSSSsttlnn;(e2 C IOOWWW8h.H$&\ۻw簾&6' B'翧<5Q/C( ׂhJR"| 0hn߾]]]]__#*gϞS!rpB+--(ŋ B"444\pĉUUUhhSSǏ[[[IFq8@ʀR] FjߖTڦ춶b&8h[->>ݻ42]܉JщǏohhLIIq8p7٦3`xا'Q]zw|٩r@@}eR~񎎎.)HKgӔd̀$ FhO(9&0LgSkoo9fDyQ hѽJȑI$56}3 ̖]`k5K!c!ds*:EZ)Ȧ2.6l2`1]EF vtT];q'vJE> ]i> v]>*F TN?7@QT[[z|Ru|61~@`|eHu7@"rWPE|hSOQ< Ž{ ???+++nJKK">UGGyd2Yii=߻w޽{,u*4`jmmEyrrr߿fffSF|$EQ---pbC 744P(ʪʊȿ<^aaaii:L?w\/^HÏY%C 2qӦMP%KDGGÿW\ٷo۷oBl, ;w>۷<&Ih)$DDDǏy 55,g 6Pu}NͶ68p?x`8+CQԩS _lkkvtN@v* z=u|V*aaa-(͛7=2008qD4BwGˆso1455W(T='O~APZZZ?S]]D"a<MԻ_ss3g۷gΜIRqիW߼yܺukLLLTTO?zjSSӐXx UssBnݺu=-- 6,Yʪrwݼy3 ))iĉ%%%שmذʕ+ ,8ٳ:ǏuKKcMM-[>>>nݚ4i[E) >illCtQXX̙3MMM!mpp0|{T*>zP(kjjZlY^m&Hƍ'"##ǎ{ &ׯիW}H$ھ}L&2eJ]]ݽ{'NLHH3f 򂓓O>N*---..O`Y5j+ 77w֭w|֭[ω':;;׿Kcccvv6|ܹ[nM2e}3gαcۇ 2bĈ˗/{zz^tIKKk׮]sqq  ~gDR\\vZHHdzvw\P(?t%tY:b;v3f̀q;~-ZhQSS_f͚Z???uu?iiigΜ4hP=]P(njll,HR駟~o߾;vry???==7nx[:zذa֭LG CCCuuux^ٳT:eʔ?3770:::+V=zgΜ?/_)L4dAAAӦMsss3fP(LLLJrÆ &Lx:xӦMcƌILLt޽{Ν B xhhE6nܸdΝ;'N8w5k`JV32O_|ԩS'NأG6޹s:.22288855_~YlD";w޽{/^xe ??gdѣGO8~jo߾]SSsΜ9ׯ>{AN>-J'Mt7n̞=;$$`nn>j(T./^hѢK۷W^| ,,,vظzEJ$55Yf-\N痐`aa̞={ȑ:::[n|}}!bX&5j߾}2f̘GGG'&&zzz_X/^,ZhժUϟ?_pk߾}a#2QûJm{B@kkkss9|>xLP[CUDpٴiӚH9s%K(Zr%q }ccrkkkDbhh(BBBy<;('O*** rS2L]]'(+ooÇ,AW֎R,kG[6Ñ`3 JLLܵkי3g {@0uqEFFRUӈ5jhxz޽w޽vZ‰tttr(<*@L\.(+Wcbbmj:?] `ƌ/r\{{;n-[\EbtMdX \PZ 裋 X,jB< I! Ҙܼn:=##^Ňd2L| /4AG]].|???OP!z > x(t p8>0g )4eGGǯ .$$g utpp\ &li  ?p!Ic"_!ӢD HG!cQZ^-eFcݫW_5K[[ǣ1TTT4sLDZne+@h޼y*t5oA~v xȄ TAB&P( ,>>> 0p@YM=!Ewr壘m򃖋~HrG@|s<R1uUF4vIxF44nyY[[,qК җ0@lrsscbbe:l@f' rScġ48Iܬv(><\G9(JÇw)**s4:99w `(p_Zm@ @WDʏSfl97)8}Z"dG3 BuxRpCmooOKK˗/3$=Ũ>MAƲ"/cƌa*MA6_Ј|8ƨH$W|wep ٰ\#elVU^lYhiTơ -k@n8dc#K4MJJc}QR揣 hv]"EѤ-͵R1;T|z5Jioo011  ;v۷E4jd"a۶m>>>ӧOgԂ:EwNk;U355ٳ@ :t(L>}zdd$P|p4fUرC[߾EIYp(VD8]G 6C%[ A(##N4N8 hUQG2pU( D"I vϡ [6J#Á[Ap|T /`F뫮 }tT2ǘV1y`TdA-?A??W^YZZ^EGG)))Wo/\0u^z1 Ɩ;;0YZ}*Gsn[@d$A&P,_nLagUe;e&*#NV vZr8^qȀzഴիWEGG_znd]F&!655mٲ%33344n^ի{xxxzzY^LQԀ;F;t…GY`EQdqPSS~ٳg?M65SjЬT*r9H-2EܸObLHP"% FPSC0FQN8lN(F&3*H#+sP**J*޼ymkmmJ͛FqY>}z=FFFׯ_7o:Ŵ?s777x(#&^9D/^7ng}6tІ8FFCbbb\\\dwƍM6Bɿ4F$_2ETfggr/._ O>)0*-7M)v]MdL6QȮP(ȞBpqqilloWXtOAҶCQ!_]]]4G&ݸqQCCwޓ'OQFX|ȈFqWZy@2+P)Sf̘:iҤ\F(RǏ}:::=ztBB2m4۷o31}ѱcǖݽ{wȑ`aaK,Xk.T,Tih90?xL߷Buu}}}zꥩѣCBSNC(+˗DW^_tuuQ~!khh=ٙC;R Nǩʂ;p8zhSSɓ2LQkjj9۷_tuu\ڲ $:uJMMmܹory]]H$􌌌r@ 1cƭ[ [[[[[[-,,vDl*X@٨u>~)hFUUUϜY~ׯ-[vDYmФ̏ⲳ;::aI/^3ںK ƒp9s$bƖE 555cƌCE-Zȑ#>~:>  MMME"ڵk>|x" `hXR88_|1o޼? SN޽~R۷>͛gkkϝ;7bgfd0np¬YcbbNhq7oFl]ϑGi5PAsbx˖-666cǎōNDѥ𯮮ի۷ veffdggcÇ߸qcpp0.Ç?hkkW2N[[[__o.\`1` 8h&UGbLݻ9bhFl>,_={ٳgFFFvvvZZZOOÇ/_<<<֭[q(UWW1ŋ_aaa#F¦ pĆl&"OQ4Qmm-"JyMs F?C&ƞ>}:))իAAA---pWަM͛~#Gl߾'''j׮]Hɶm۶-\p,2ZlbJ*M /`ѲQO6A)mmm3jQ!W,**ɉÇ|ΝkiiH-Zha5e9;;xǖ4RAZƫ$qfRiuu5[`'֗BSѣ˖-Ԝ={vZZwHHHAA#G7}}ѣGΟ?`~9;;ٳg֬Y֋/0aBLL̈# -TD·$ u8_nx'vt---kkk;;;ƐM돦 z+Hf̘a``O?YXX\rc@VVցcddwj^E"AY Ŋ+<<}H񯽽L&)gΜJHHÉ @ SRTK./Z_rLN(~wǏ߰aڵkLLLLLLn#ݒp .IY(FGG}&&&E={lpǏ( 3MT:~WZ[[Ϝ9s޽0A kkk+**hGhѢCݻ722;vXhQeee~ -)0zXIypc 4ʀMJJАJ@akkښaÆE®Q) )MA!CP(˖-S*Og$&&¶ ͛#Fϟ9sё-^ XCv=zk ̦#p`*dc 'pƦVKKԴ"!!ݏѳgO777tEQ~ɱc&$$l޼nnn,2O> l,4556G BGGGGGQFe^jjjZZZԠAJ`6ʸ x555^T;%TzQԸ//^/ YtrBHҖ@VVֱcǦOnhhnҥ7n]tuu>pBN NA2UWWƒttt̂ZZZ\'< E|}9m۶555YXX5l߾}͚5p]L&u떁A~~˗/o޼sΆ=z #""e3WT񇷷7nkF`4oyyy^(իW߾}|۷o8i{^I`d_QQ1aF"ĨX( ^I"O8w/$bqi9||| رc?Zd̙111_}UMMѣ̙3|pZ./YrÆ _}ʕ+=ʆj*r?Bޡ Z(dۉD"ݠj50;rȿiƍ dXgjjtR\ooo/vtt>sP(߾[PH(DR]]CMBnpK1N:uT"6TٳgHĪ;wgϞM>V$[ŋB"׬W4 xӧwA]544:_~{9~~ k Nfyǟ9s/YfN_1f^܃Ɯ{#8]\&FRu!C|NNN/e[8ƔMMӧ۱cG``H$JHH ^ڸqǏ EKKKaaa^^˗/Š曩S&&&~,SSS###MMӧOZ lM RTd"h}P(433& iO"KƎ NKK|www[[[KKˊ +++@ŋ+W455566 B8ֶ{n؜2Pl=<_VVVFFF #e##ݻw3{{WFEEEEE=ƅVyȑ555###8 RΝ;+++KDRWWАP[[rʀ55q _pܹss[%wTX|FlHxO[%{.`jjA[@```o3'mɒ% ,(**__x|ٳgse217f7<cG©#Xp̛7oZYY8x\h'$82jii3"p@'8p͛<ٓ'O-[flllggIcTPP02*ΦÀ, [[[QGQTZZl :mBCCr@=+"##92{ltOWWW'7}@|||ݿ}p-/_5k֩S,Ynii:v333 ÐȥK3 ?(:垗[VV?D *@`Λ7ȑ#:::Bn{mےvIstt>|8>(/gcVۧO)Sc={x< 3@ 즦7n/hȘDDMcbWڵkӦM9r#^z9٩$Ǐ5kVFF,SQQڊ0y<ދ/⋋/{^I 9)gFFF۶mwYbiBՉ˰0yo߾Gw999+.--H&6P'''D#H_[xCyɏ=JOO>{s.\FjVdl)zܹy%%%JիxǏ/^8,,O>$Y)}AK.SRRJX,.\8FaJGGǍ7SSS?FDD|'lFϟ?U뫣+f$/q"066ߴiSll,/EN/#ʼnlzj(׷%dvUI^SS>zQ0,\_ "vsy>$&&>|eHHH@8F4ݻiHd`fffTTTGGsƌ8 J<:2bZGF_5999$!!a׮]qqqfTWW nR):I[1.]iӦK8R,4ni+~wn!\ZZZXXԄ/zee#G߿/JO,YB*Ȱ 322ljp8}Ϻ:ƴ{9Ν;yffuu-[˫ۢA=N8q 6? C++HMM۷omm-[%QKKKʜV\y-SSӉ&uJ*9?J*<| .6vĉãBpYܼYT Bccc ɨ V&\.$wguΞ=[YYyE@mc}066Xnݜ9s9c9HQe2\.h/vT*|>_$Vjnn~9y#N=zt?w}v ? 8SÎ0a» ou !Cܾ}8::믿oCp; r& 6h1![ٳg۶mv 2;cY]]!oDDĖ-[V\yر'N06 bԩ~ȑ#% ͒ D>} 9!@#B %gxjjg}{exx޼yCځP*'N;w.  fF:$A6iLqH|6ΦV&wܸq[nEǮK$O>ٳg-***44]D~U'bm*Qh$%q /ss;v8Q[[[+䬬,555X,߾}+JRikk+-cbbrrr;(%f᭭IIIEEE|d$ϨDܻw `<]rĈiii+Wևɓ' An6sg޻;zبDاi8b~LJK1n rd=~U||3FFnlG4TQm[9pylll>}Z__ {{{;;;׿%~f .\X\\7߰ P-dSC ]¶nݺ|iӦ1b}YY?yf@:FFF.];z_|oǏG_0HTVVgjjW^/C7nX~=< 2)*iyy baʂQXHGFFFdF䐁[8ĠApppQQQHH766VSSׯߵkbbbϟ?xիWI?ccc3334T*=z}vHo>ggg… ?~qƱcvcl777Xn$2e䧟gմ0=btZ6VVVʂ7Coѹ[@5UCCto֬YÁH1zEF;m]meKJJlmm߼yɎ1޽{G>rWq;vl׮]NrrrIDEE=zthwCKDyyy|Uԡյ^ZKKwhHKu QE.Y__U"Q7O> 1#!Fܸ[Įv6C[;;;x'pYiϸm&D950Ց#GشcֆG3 Iذa7nX`ӧO'NAF( g$͛332,/_ٜA(d^L+9 i]+H?\PPp!t&x di3vd塱#+ `ViL9["IM6z#R333z [HtJ84fx%%%4k WWWgggx Ĩ Iy6UHnPLe ..={odd!CVSvS b6" MAbt[A?XXX"sst;;;?~LީT6dUŁF2ZJ'&!#S|;iҤ'Ndgg[]2GKJJ4h޽{E4a>*J:#*2SDdlDunMMMx@, %%%FFF |x^QPŋRƋx=z'YtZqK ﯠ CV6~*æ3c^UZ&Z@3J&7 4wMʘOԞpi[i8 f *u/+FC{^%QU!>|wBeLx.2#i;pLmccG#HjAYH;PA&* Y͝(fIF q$v8)AR;Iu[AΌO8'Ԃr'I"4S^Jt3c9VET6vt9иMA0gL[AȢ 抔@ 텄nȿe{q4'6P%d{ŭ$?nvj)VAUJ3_nQг,$Wy+SWwJ ]~7wU|O*>~ 2pg۷o9zR^Q p#( \/ZxOR+ÊJKKϩ@K.5kѣG~혘.ݻw\dڳgONNe6ެm-Xxbv= n 驫c Hcv]SScZ|qgq5. V ^[V^^NȚ\Vb)--GgS.X#677L&ZrZJ.pqjh4뫪b%۷ hd뎎&)R c%xΝVsƹz!Ai>6Wb_'OI1dmyb7^fmZ+AJHJsT& ㅅ<289sFqx<ׯ_gZ$ܺ:.+ǎkii=vأGX 3;;74јcZL\Ǐ2VUU8q딦pxD 'NsNAA ^OMLRۙq?}tII q4AMMMǏSAD__-g EO:Ey< :.???n JV5''`0@0,???1A/N, tww7552vW$aS%!!Փ.f([~͛7A@Q4++. N8AI3QQQUUUYYY٠1WP333srr^^sN{|ffǏ'''|riC?d(aիV3&i"b1 ?z'NuV\sPgΜINNvEիWgeey=<<,HU*W.aXRutt,]\!r[ZmBB‚ %jtRy`HN,jx<3g4o̙36mڟjժXƮYPnݺ=sիWduR`6uuuv]ծ[N&Q##˧MNaAԦ+W, XhH}M-UFRuvv.YðnAϟl2?*..VsΥ^p^OKKKJJgBBBq_v-m* ٳgyyy}Yrr2y AEEE&7LKKH$$S .^aT* π@}}}[[X,LLL$D9%%?##cѢEb0^UUbŊ+W9522"GuuuTTTff&M6͙3k N?_Cbi% xZ#!bƌ۶mvnݺ1>>@  ^g.40,ɦOrȠ /Yd@Vÿ꫘(J$uVժjuI$9s( _X,v:ӧO,ið]v9NLvpRW3/LP Njb2:AN{{{dd$Z.\'ɦNJJL& ي|wÃaJK@@9⋰HBA%"waXfϞ4cǎF ? b޽{ɒ%TVS w}#R)'Ο?T*IOm۶EEE͞=#ݸqc\\br~@87y  |hNb\.DOO㡽\3fxM9V `F'X)D! 5dʈD"C ȷV[ZZ Q0 S*}nݺh&L[>Nd4Lq\T+V˗/o߾b@ٹxbJn;Na \.Wyy9aK.&t(ǥ+A qon߹sٳg}||NglllMMbIOOldʔ)n{N:>>V5!!r%&&:hTРT*yOb`#""֯_v; iii.\P(smoo7oFQtժUޕ+Wn)SBCC JB$!!b&&&y pXVNc\^^xZZht8@0!SG^`Eaxt+/|=22ɓᬬ,Іc6|A@U-cbb BD ׮] _#b4333JԔ)S*++e2 kllLJJaX477nV;888!`0̜9>UTQQQUUUdH$dLLH$Jt:Hx<+W̘1?"""** ð OSF#ەOA^ϘrO<H$bB N$ZLy!D"jzpp`0I$EikDn Sy!gljGFF]0͡4և^ymЋf3 x}+$&?$&A> endobj 358 0 obj << /Length 22069 >> stream 0.271 0.267 0.267 rg q 15.000 44.625 577.500 732.375 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that the maximum length of both the PR-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(alone and the FR5/PR test sessions was reduced to 60 min. The FR5/PR sessions still began with 10 min of the FR5 schedule, )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(now followed by 50 min of PR training. Animals in this experiment received 5 sessions of FR5 training following acquisition of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(the lever press, followed by 10 sessions of PR-alone training and concluding with 8 sessions of the mixed FR5/PR procedure.)] TJ ET BT 26.250 712.357 Td /F5 9.8 Tf [(Behavioral Results)] TJ ET BT 26.250 692.952 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 692.952 Td /F1 9.8 Tf [( All mice acquired the lever press within two days with the exception of one female z_Q175 het animal, which )] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(required 6 days. While WT mice increased their response rate as training progressed, responding in the z_Q175 mice )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(responding was lower on all days and did not change with training \(Genotype x Session interaction: F\(4,67\) = 2.66, )] TJ ET BT 523.471 669.143 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 558.980 669.143 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(simple main effects: smallest F\(1,67\)= 5.17, all )] TJ ET BT 230.259 657.238 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 270.644 657.238 Td /F1 9.8 Tf [(; Session effect for the WT: F\(4,67\) = 10.5, )] TJ ET BT 457.882 657.238 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 504.234 657.238 Td /F1 9.8 Tf [(; not shown\).)] TJ ET BT 26.250 637.833 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 637.833 Td /F1 9.8 Tf [(z_Q175 het mice earned fewer reinforcers across the PR training sessions than did WT controls, although both )] TJ ET BT 26.250 625.929 Td /F1 9.8 Tf [(groups earned more as training progressed \(Genotype main effect: F\(1,17\) = 38.0)] TJ ET BT 378.771 625.929 Td /F5 9.8 Tf [(, p < 0.0001;)] TJ ET BT 433.254 625.929 Td /F1 9.8 Tf [( Session main effect: F\(9,153\) = )] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(13.7, )] TJ ET BT 50.645 614.024 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 102.417 614.024 Td /F1 9.8 Tf [(Figure 4, right panel\))] TJ ET BT 192.370 614.024 Td /F5 9.8 Tf [(. )] TJ ET BT 197.791 614.024 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers \(Sex main effect: F\(1,17\) = 11.3, )] TJ ET BT 507.471 614.024 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 548.402 614.024 Td /F1 9.8 Tf [(not )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(shown\).)] TJ ET BT 26.250 582.714 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 582.714 Td /F1 9.8 Tf [( During the FR5 component of the FR5/PR phase, the total number of reinforcers earned in both groups )] TJ ET BT 26.250 570.810 Td /F1 9.8 Tf [(increased and then decreased across sessions, z_Q175 het mice earning fewer reinforcers than did WT mice overall \(Genotype )] TJ ET BT 26.250 558.905 Td /F1 9.8 Tf [(main effect: F\(1,17\) = 4.75, )] TJ ET BT 146.292 558.905 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 181.802 558.905 Td /F1 9.8 Tf [(; Session main effect: F\(7,119\) = 8.27, )] TJ ET BT 350.077 558.905 Td /F5 9.8 Tf [(p < 0.0001;)] TJ ET BT 399.139 558.905 Td /F1 9.8 Tf [(Figure 5, upper right panel\).)] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(In the PR component the same pattern was shown across sessions, with fewer reinforcers earned with more training and )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(reduced responding in the mutants \(Genotype main effect: F\(1,17\) = 10.2,)] TJ ET BT 344.646 527.595 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 385.577 527.595 Td /F1 9.8 Tf [(Session main effect: F\(7,119\) = 14.7, )] TJ ET BT 548.431 527.595 Td /F5 9.8 Tf [(p < )] TJ ET BT 26.250 515.691 Td /F5 9.8 Tf [(0.001; )] TJ ET BT 56.066 515.691 Td /F1 9.8 Tf [(Figure 5, bottom right panel)] TJ ET BT 175.288 515.691 Td /F5 9.8 Tf [(\). )] TJ ET BT 183.956 515.691 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers than did males \(Sex main effect: F\(1,17\) = 4.58, )] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 67.180 503.786 Td /F1 9.8 Tf [(not shown\).)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(During the FR5 period, the overall response rate for both groups was lower within sessions as more reinforcers were earned, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(with the z_Q175 het mice responding significantly less for almost all reinforcers \(Genotype x Reinforcer Bin interaction: )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(F\(29,493\) = 3.31, )] TJ ET BT 104.026 460.572 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 150.377 460.572 Td /F1 9.8 Tf [(. Simple main effect analysis for reinforcers 1-14, 16-17, 19: smallest F\(1,493\) = 5.58, all )] TJ ET BT 534.303 460.572 Td /F5 9.8 Tf [(ps < 0.02;)] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(Figure 6, upper right panel\). There were no sex effects.)] TJ ET BT 26.250 429.262 Td /F1 9.8 Tf [(Finally, response rate as a function of response requirement within sessions in the PR component of the FR5/PR phase, as in )] TJ ET BT 26.250 417.357 Td /F1 9.8 Tf [(Experiment 2A, showed an inverse U-shaped response function for both groups with significantly lower overall responding in the )] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(z_Q175 mice but no significant interaction with the required level of effort \(Genotype main effect: F\(1,19\) = 9.89, )] TJ ET BT 511.546 405.453 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 547.056 405.453 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(Response Requirement main effect: F\(10,192\) = 9.92, )] TJ ET BT 262.259 393.548 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 308.610 393.548 Td /F1 9.8 Tf [(; Figure 6 lower right panel\). There were no sex effects.)] TJ ET BT 26.250 356.945 Td /F4 12.0 Tf [(Power Analyses)] TJ ET BT 26.250 336.991 Td /F1 9.8 Tf [(In order to evaluate the utility of this procedure for screening work, power analysis was carried out on the Genotype main effect )] TJ ET BT 26.250 325.086 Td /F1 9.8 Tf [(and the Genotype x Response Requirement interaction in data from the PR period of the final FR5/PR period of each of the four )] TJ ET BT 26.250 313.182 Td /F1 9.8 Tf [(experiments. The output of these analyses is summarized in Table 1 below, indicating that the assay has reasonable power in )] TJ ET BT 26.250 301.277 Td /F1 9.8 Tf [(both lines tested. In the BAC HD mice, around 35 mice per group are required for a 50% effect size in the Genotype x )] TJ ET BT 26.250 289.372 Td /F1 9.8 Tf [(Response Requirement interaction. For the z_Q175 het animals, around 36 mice per condition are required, again for a 50% )] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(effect size but here with the better power seen in the overall Genotype effect, reflecting the more global phenotype seen in this )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(line.)] TJ ET q 450.000 0 0 147.000 26.250 108.682 cm /I52 Do Q BT 26.250 91.658 Td /F5 9.8 Tf [(Table 1: Required n/condition for 80% power with the effect sizes specified.)] TJ ET BT 26.250 55.055 Td /F4 12.0 Tf [(Discussion)] TJ ET Q q 15.000 44.625 577.500 732.375 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that the maximum length of both the PR-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(alone and the FR5/PR test sessions was reduced to 60 min. The FR5/PR sessions still began with 10 min of the FR5 schedule, )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(now followed by 50 min of PR training. Animals in this experiment received 5 sessions of FR5 training following acquisition of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(the lever press, followed by 10 sessions of PR-alone training and concluding with 8 sessions of the mixed FR5/PR procedure.)] TJ ET BT 26.250 712.357 Td /F5 9.8 Tf [(Behavioral Results)] TJ ET BT 26.250 692.952 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 692.952 Td /F1 9.8 Tf [( All mice acquired the lever press within two days with the exception of one female z_Q175 het animal, which )] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(required 6 days. While WT mice increased their response rate as training progressed, responding in the z_Q175 mice )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(responding was lower on all days and did not change with training \(Genotype x Session interaction: F\(4,67\) = 2.66, )] TJ ET BT 523.471 669.143 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 558.980 669.143 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(simple main effects: smallest F\(1,67\)= 5.17, all )] TJ ET BT 230.259 657.238 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 270.644 657.238 Td /F1 9.8 Tf [(; Session effect for the WT: F\(4,67\) = 10.5, )] TJ ET BT 457.882 657.238 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 504.234 657.238 Td /F1 9.8 Tf [(; not shown\).)] TJ ET BT 26.250 637.833 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 637.833 Td /F1 9.8 Tf [(z_Q175 het mice earned fewer reinforcers across the PR training sessions than did WT controls, although both )] TJ ET BT 26.250 625.929 Td /F1 9.8 Tf [(groups earned more as training progressed \(Genotype main effect: F\(1,17\) = 38.0)] TJ ET BT 378.771 625.929 Td /F5 9.8 Tf [(, p < 0.0001;)] TJ ET BT 433.254 625.929 Td /F1 9.8 Tf [( Session main effect: F\(9,153\) = )] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(13.7, )] TJ ET BT 50.645 614.024 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 102.417 614.024 Td /F1 9.8 Tf [(Figure 4, right panel\))] TJ ET BT 192.370 614.024 Td /F5 9.8 Tf [(. )] TJ ET BT 197.791 614.024 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers \(Sex main effect: F\(1,17\) = 11.3, )] TJ ET BT 507.471 614.024 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 548.402 614.024 Td /F1 9.8 Tf [(not )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(shown\).)] TJ ET BT 26.250 582.714 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 582.714 Td /F1 9.8 Tf [( During the FR5 component of the FR5/PR phase, the total number of reinforcers earned in both groups )] TJ ET BT 26.250 570.810 Td /F1 9.8 Tf [(increased and then decreased across sessions, z_Q175 het mice earning fewer reinforcers than did WT mice overall \(Genotype )] TJ ET BT 26.250 558.905 Td /F1 9.8 Tf [(main effect: F\(1,17\) = 4.75, )] TJ ET BT 146.292 558.905 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 181.802 558.905 Td /F1 9.8 Tf [(; Session main effect: F\(7,119\) = 8.27, )] TJ ET BT 350.077 558.905 Td /F5 9.8 Tf [(p < 0.0001;)] TJ ET BT 399.139 558.905 Td /F1 9.8 Tf [(Figure 5, upper right panel\).)] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(In the PR component the same pattern was shown across sessions, with fewer reinforcers earned with more training and )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(reduced responding in the mutants \(Genotype main effect: F\(1,17\) = 10.2,)] TJ ET BT 344.646 527.595 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 385.577 527.595 Td /F1 9.8 Tf [(Session main effect: F\(7,119\) = 14.7, )] TJ ET BT 548.431 527.595 Td /F5 9.8 Tf [(p < )] TJ ET BT 26.250 515.691 Td /F5 9.8 Tf [(0.001; )] TJ ET BT 56.066 515.691 Td /F1 9.8 Tf [(Figure 5, bottom right panel)] TJ ET BT 175.288 515.691 Td /F5 9.8 Tf [(\). )] TJ ET BT 183.956 515.691 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers than did males \(Sex main effect: F\(1,17\) = 4.58, )] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 67.180 503.786 Td /F1 9.8 Tf [(not shown\).)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(During the FR5 period, the overall response rate for both groups was lower within sessions as more reinforcers were earned, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(with the z_Q175 het mice responding significantly less for almost all reinforcers \(Genotype x Reinforcer Bin interaction: )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(F\(29,493\) = 3.31, )] TJ ET BT 104.026 460.572 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 150.377 460.572 Td /F1 9.8 Tf [(. Simple main effect analysis for reinforcers 1-14, 16-17, 19: smallest F\(1,493\) = 5.58, all )] TJ ET BT 534.303 460.572 Td /F5 9.8 Tf [(ps < 0.02;)] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(Figure 6, upper right panel\). There were no sex effects.)] TJ ET BT 26.250 429.262 Td /F1 9.8 Tf [(Finally, response rate as a function of response requirement within sessions in the PR component of the FR5/PR phase, as in )] TJ ET BT 26.250 417.357 Td /F1 9.8 Tf [(Experiment 2A, showed an inverse U-shaped response function for both groups with significantly lower overall responding in the )] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(z_Q175 mice but no significant interaction with the required level of effort \(Genotype main effect: F\(1,19\) = 9.89, )] TJ ET BT 511.546 405.453 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 547.056 405.453 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(Response Requirement main effect: F\(10,192\) = 9.92, )] TJ ET BT 262.259 393.548 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 308.610 393.548 Td /F1 9.8 Tf [(; Figure 6 lower right panel\). There were no sex effects.)] TJ ET BT 26.250 356.945 Td /F4 12.0 Tf [(Power Analyses)] TJ ET BT 26.250 336.991 Td /F1 9.8 Tf [(In order to evaluate the utility of this procedure for screening work, power analysis was carried out on the Genotype main effect )] TJ ET BT 26.250 325.086 Td /F1 9.8 Tf [(and the Genotype x Response Requirement interaction in data from the PR period of the final FR5/PR period of each of the four )] TJ ET BT 26.250 313.182 Td /F1 9.8 Tf [(experiments. The output of these analyses is summarized in Table 1 below, indicating that the assay has reasonable power in )] TJ ET BT 26.250 301.277 Td /F1 9.8 Tf [(both lines tested. In the BAC HD mice, around 35 mice per group are required for a 50% effect size in the Genotype x )] TJ ET BT 26.250 289.372 Td /F1 9.8 Tf [(Response Requirement interaction. For the z_Q175 het animals, around 36 mice per condition are required, again for a 50% )] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(effect size but here with the better power seen in the overall Genotype effect, reflecting the more global phenotype seen in this )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(line.)] TJ ET q 450.000 0 0 147.000 26.250 108.682 cm /I54 Do Q BT 26.250 91.658 Td /F5 9.8 Tf [(Table 1: Required n/condition for 80% power with the effect sizes specified.)] TJ ET BT 26.250 55.055 Td /F4 12.0 Tf [(Discussion)] TJ ET Q q 15.000 44.625 577.500 732.375 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(All equipment and behavioral procedures were as described in Experiment 1A, except that the maximum length of both the PR-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(alone and the FR5/PR test sessions was reduced to 60 min. The FR5/PR sessions still began with 10 min of the FR5 schedule, )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(now followed by 50 min of PR training. Animals in this experiment received 5 sessions of FR5 training following acquisition of )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(the lever press, followed by 10 sessions of PR-alone training and concluding with 8 sessions of the mixed FR5/PR procedure.)] TJ ET BT 26.250 712.357 Td /F5 9.8 Tf [(Behavioral Results)] TJ ET BT 26.250 692.952 Td /F5 9.8 Tf [(Pretraining.)] TJ ET BT 76.102 692.952 Td /F1 9.8 Tf [( All mice acquired the lever press within two days with the exception of one female z_Q175 het animal, which )] TJ ET BT 26.250 681.048 Td /F1 9.8 Tf [(required 6 days. While WT mice increased their response rate as training progressed, responding in the z_Q175 mice )] TJ ET BT 26.250 669.143 Td /F1 9.8 Tf [(responding was lower on all days and did not change with training \(Genotype x Session interaction: F\(4,67\) = 2.66, )] TJ ET BT 523.471 669.143 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 558.980 669.143 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 657.238 Td /F1 9.8 Tf [(simple main effects: smallest F\(1,67\)= 5.17, all )] TJ ET BT 230.259 657.238 Td /F5 9.8 Tf [(ps < 0.05)] TJ ET BT 270.644 657.238 Td /F1 9.8 Tf [(; Session effect for the WT: F\(4,67\) = 10.5, )] TJ ET BT 457.882 657.238 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 504.234 657.238 Td /F1 9.8 Tf [(; not shown\).)] TJ ET BT 26.250 637.833 Td /F5 9.8 Tf [(Simple PR. )] TJ ET BT 77.720 637.833 Td /F1 9.8 Tf [(z_Q175 het mice earned fewer reinforcers across the PR training sessions than did WT controls, although both )] TJ ET BT 26.250 625.929 Td /F1 9.8 Tf [(groups earned more as training progressed \(Genotype main effect: F\(1,17\) = 38.0)] TJ ET BT 378.771 625.929 Td /F5 9.8 Tf [(, p < 0.0001;)] TJ ET BT 433.254 625.929 Td /F1 9.8 Tf [( Session main effect: F\(9,153\) = )] TJ ET BT 26.250 614.024 Td /F1 9.8 Tf [(13.7, )] TJ ET BT 50.645 614.024 Td /F5 9.8 Tf [(p < 0.0001; )] TJ ET BT 102.417 614.024 Td /F1 9.8 Tf [(Figure 4, right panel\))] TJ ET BT 192.370 614.024 Td /F5 9.8 Tf [(. )] TJ ET BT 197.791 614.024 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers \(Sex main effect: F\(1,17\) = 11.3, )] TJ ET BT 507.471 614.024 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 548.402 614.024 Td /F1 9.8 Tf [(not )] TJ ET BT 26.250 602.119 Td /F1 9.8 Tf [(shown\).)] TJ ET BT 26.250 582.714 Td /F5 9.8 Tf [(Mixed FR5/PR.)] TJ ET BT 92.345 582.714 Td /F1 9.8 Tf [( During the FR5 component of the FR5/PR phase, the total number of reinforcers earned in both groups )] TJ ET BT 26.250 570.810 Td /F1 9.8 Tf [(increased and then decreased across sessions, z_Q175 het mice earning fewer reinforcers than did WT mice overall \(Genotype )] TJ ET BT 26.250 558.905 Td /F1 9.8 Tf [(main effect: F\(1,17\) = 4.75, )] TJ ET BT 146.292 558.905 Td /F5 9.8 Tf [(p < 0.05)] TJ ET BT 181.802 558.905 Td /F1 9.8 Tf [(; Session main effect: F\(7,119\) = 8.27, )] TJ ET BT 350.077 558.905 Td /F5 9.8 Tf [(p < 0.0001;)] TJ ET BT 399.139 558.905 Td /F1 9.8 Tf [(Figure 5, upper right panel\).)] TJ ET BT 26.250 539.500 Td /F1 9.8 Tf [(In the PR component the same pattern was shown across sessions, with fewer reinforcers earned with more training and )] TJ ET BT 26.250 527.595 Td /F1 9.8 Tf [(reduced responding in the mutants \(Genotype main effect: F\(1,17\) = 10.2,)] TJ ET BT 344.646 527.595 Td /F5 9.8 Tf [(p < 0.01; )] TJ ET BT 385.577 527.595 Td /F1 9.8 Tf [(Session main effect: F\(7,119\) = 14.7, )] TJ ET BT 548.431 527.595 Td /F5 9.8 Tf [(p < )] TJ ET BT 26.250 515.691 Td /F5 9.8 Tf [(0.001; )] TJ ET BT 56.066 515.691 Td /F1 9.8 Tf [(Figure 5, bottom right panel)] TJ ET BT 175.288 515.691 Td /F5 9.8 Tf [(\). )] TJ ET BT 183.956 515.691 Td /F1 9.8 Tf [(Female mice earned fewer reinforcers than did males \(Sex main effect: F\(1,17\) = 4.58, )] TJ ET BT 26.250 503.786 Td /F5 9.8 Tf [(p < 0.05; )] TJ ET BT 67.180 503.786 Td /F1 9.8 Tf [(not shown\).)] TJ ET BT 26.250 484.381 Td /F1 9.8 Tf [(During the FR5 period, the overall response rate for both groups was lower within sessions as more reinforcers were earned, )] TJ ET BT 26.250 472.476 Td /F1 9.8 Tf [(with the z_Q175 het mice responding significantly less for almost all reinforcers \(Genotype x Reinforcer Bin interaction: )] TJ ET BT 26.250 460.572 Td /F1 9.8 Tf [(F\(29,493\) = 3.31, )] TJ ET BT 104.026 460.572 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 150.377 460.572 Td /F1 9.8 Tf [(. Simple main effect analysis for reinforcers 1-14, 16-17, 19: smallest F\(1,493\) = 5.58, all )] TJ ET BT 534.303 460.572 Td /F5 9.8 Tf [(ps < 0.02;)] TJ ET BT 26.250 448.667 Td /F1 9.8 Tf [(Figure 6, upper right panel\). There were no sex effects.)] TJ ET BT 26.250 429.262 Td /F1 9.8 Tf [(Finally, response rate as a function of response requirement within sessions in the PR component of the FR5/PR phase, as in )] TJ ET BT 26.250 417.357 Td /F1 9.8 Tf [(Experiment 2A, showed an inverse U-shaped response function for both groups with significantly lower overall responding in the )] TJ ET BT 26.250 405.453 Td /F1 9.8 Tf [(z_Q175 mice but no significant interaction with the required level of effort \(Genotype main effect: F\(1,19\) = 9.89, )] TJ ET BT 511.546 405.453 Td /F5 9.8 Tf [(p < 0.01)] TJ ET BT 547.056 405.453 Td /F1 9.8 Tf [(; )] TJ ET BT 26.250 393.548 Td /F1 9.8 Tf [(Response Requirement main effect: F\(10,192\) = 9.92, )] TJ ET BT 262.259 393.548 Td /F5 9.8 Tf [(p < 0.0001)] TJ ET BT 308.610 393.548 Td /F1 9.8 Tf [(; Figure 6 lower right panel\). There were no sex effects.)] TJ ET BT 26.250 356.945 Td /F4 12.0 Tf [(Power Analyses)] TJ ET BT 26.250 336.991 Td /F1 9.8 Tf [(In order to evaluate the utility of this procedure for screening work, power analysis was carried out on the Genotype main effect )] TJ ET BT 26.250 325.086 Td /F1 9.8 Tf [(and the Genotype x Response Requirement interaction in data from the PR period of the final FR5/PR period of each of the four )] TJ ET BT 26.250 313.182 Td /F1 9.8 Tf [(experiments. The output of these analyses is summarized in Table 1 below, indicating that the assay has reasonable power in )] TJ ET BT 26.250 301.277 Td /F1 9.8 Tf [(both lines tested. In the BAC HD mice, around 35 mice per group are required for a 50% effect size in the Genotype x )] TJ ET BT 26.250 289.372 Td /F1 9.8 Tf [(Response Requirement interaction. For the z_Q175 het animals, around 36 mice per condition are required, again for a 50% )] TJ ET BT 26.250 277.467 Td /F1 9.8 Tf [(effect size but here with the better power seen in the overall Genotype effect, reflecting the more global phenotype seen in this )] TJ ET BT 26.250 265.563 Td /F1 9.8 Tf [(line.)] TJ ET q 450.000 0 0 147.000 26.250 108.682 cm /I56 Do Q BT 26.250 91.658 Td /F5 9.8 Tf [(Table 1: Required n/condition for 80% power with the effect sizes specified.)] TJ ET BT 26.250 55.055 Td /F4 12.0 Tf [(Discussion)] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(8)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 359 0 obj << /Type /XObject /Subtype /Image /Width 600 /Height 196 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 600 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 518>> stream xA 0 =" ʝ<2;EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$) endstream endobj 360 0 obj << /Type /XObject /Subtype /Image /Width 600 /Height 196 /SMask 359 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 600 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 79658>> stream x}gxT:3)LzޛzG,(\Ć]{-XbWP5*Bzg2ICȰ>{L>C}^'yy8== ӏd6 Űb0VÙU0fߩ_c+$BPCOrt0$۶v-A@+}PCܲE0`w?B~VH:b#EYBQ B٨0 O%Ǝ"Ӧf#tVH"VA"$2fW3}>rS#;>]1)/%Eɟ"aq/mm\1ݱCU"H,+0]jZ>KU ()&%#tyLUs $cE1GV$ŋT0P`QOOF!l9ΈPV1ɐ(dFi@71U(2Pը Bjb H*&1D,Oj+F:]9ǜK ~EPquAYQazYK]O!) U wF5$^BAN OR b䉩V *TdD5,@؀=<(^.Ȳ"7EbBHnSjЊBdU,j9n#<B* &AL #eƧtK fQ<cB*wF@9*`~DP";i#DQV<[j `bH!Bs0 Ȟ'Mo>1- )E$O6U"86qiU}⦮)˒ i|B>dֽz_ȟ(s4PNޢmx0V-b*&K&$[KMa$d"IL8f71LFV a:ɸ峒\V4/+S *VPdG62 ; Y#@"ܦbX 3=64-v{BY Y] ۆ[xl0ԏ F=LY'R6<<&Ԏ YGWͱ=jr `?>ݵM io ϖQSgN%Rd#= _G,.Gyz v *~[3*̅' Y!hX݉9y{8D’D楺HPALŬ#!3 Ohhc 5WmvU;ݰbPixd7P!Fz}gmK'>|n7(]5|U1YU4O8^tf7*_=cȠ=ijëɃœ\C\:^:FNr׫O=TUW颐sPˣo\]EW.xSɌ&)kٸg|6){bF9`tQ(Ю,4Jlr'HQ@/Lf/P&UE\h3 dYժ%Ҏ~)p#dSR'rVBԏ9 )pUVS%d7G^uYq<]Lz@qfk;?;@PyxiT/WFEE&`όpWBRzxǰ _\ҭڇ[P0SPPcD{3vwԗT5e4U{f,RC e oS^*$9lAC^f Cr#H fk t`#s1S+. ~#-5HlEeSُ>Jߐ ZX  bPٝhۦsO[~erguZKog #3;GU|$~E)T_j'Wcmz_; \gcjID& f"nVVh}bE0C4!/d̪lC @SHAb.Ғhzcb(fD@s/ hjS#OoFZdl)T밤P֪>~ZuAh ZюD7`)\rM6 $ 9,ta?@ 8ZΒIA7`QT`g=\^I"{iӆLGLqM<1`f\a@E0HԯTVTRuBo I 4aQ#Ba,B#BH W `|Ia$ sDZ#TMSȒŊTS751)]~˜GVMzؚ^)aF2'sc3JBjSȡB0ҿW2@PK @BrG/&L~ُ$j2+E3YcɟԧTbOk@ealzr.Ћ#41BAh@vHVe F~%hf^dA#J- h"G^ 2J}T,ై`/~'0NFSxPA(˵z²ny䢊`) ? R6i-V]l u(y,TxV_wJ۰f9br>$w"B,Bz;$.Tl@4Wv)2A9AG 1|Մ) BH @;$  =yQy3fj2P`PC~j Mzjy1.iq, I/P-O%_(-lڊ8Iؔw@x6Tc GfF6MAOL:Ɗ3#f^# d _+ DQkUMt'T`F%#-iOӛat*;NHx LH8m5}l ‘,&m2f##`Т/O i;0;3rb#.h'}2U}1嗺7<_Eǎgw?Oغ2Z&\o^#ϊ7lm66f"ܛ:M; /}"@>" BP!lLQ$OB$aAzHO9/zN;Z6>}/nwv;^F /≔%^{U&K_u׽o Ax8B &Vő_I)u4`;KwW~l+Lhj>H~*`{^_p—~>r}1so,:1ųXRP@d˚  奔~:ZNQ_ɸ%{0UYr|G5?K,׮on/立 ~)4s[fYtPa&b zgf7+!T~M<ӬNw4Ơf8[G; Cʲı5bi\֪[BK;vSw Um= bx 1aGӒv=ߑzFab/I0zYx3JMm5k};HMRQ_xXcڵN+GŹ;` b5дEbw24tɓ'O^g*.|fɓi{Dqniиཱུ wߵUյy=׸}wSMGO]Γ]X,x׉f}0@ PハV,}vyT^~'i'+ݟn9az.V,X3Iww ,YʼnDܖO]\un7l&J{ht\ȗ=a\Ǜ !u-Yr@ 3FM@uQe3#'Mqwroy0N|xԕkxeJDDE/|uK6!"""b:4& i-/Mt+sMw'ʊ2bBDDDDDĚ}.݅/t>j]oy d+J@~#1ֶsSmyCssP`ͫos6PZ;'~ݏO}/@O1+-&v8C]|J~+_;^+yK.XyGx^P6;j_99]&ʘYS'N5G߽zO:~rqmNQzwk,^@zZqC:$.U W\TDΘ6iꨡw=dZAhе6;.?S`p9P{wYZKiC: DZ0¹z}ٷZN>2"ǹ6k_9Q# ey#o/äbb" Kۥˎw߾zwWЙsq۩s$KSdzrR_gmcm9mȼe2NS$st0gW4BqKg}XOqnٗzܹWM&CŃU5n.M6)>ܹ^uHIu%z鈅.& 죑pWpe_Qs/m;zر}x={v_,Mݻ'E:`b bc^}tYIn>eU-mpw4=&]fS}#=~禪I]7:.$Md4@ ț~{C4Obί3ƥ8ZAQWOzC,9|{W=gjO<ݧ$Q*8CZNaQu3_>xbOB Z>Ah WykdP:) yEie :TƎJEH& S嚷s}R;O'Ҏ>53v^lʲ:wNnJCV~K9?džtu{89W}dF/Z.K!*U;iD*#"[!Y !vznLX#^|nڍǂ=r_A7U59ԓГTL?v Vzl4g9EL}Ejd2vm=ys#wL?ibTi6gJU|hgPGSS^yUnƩC8;65fV̜M`2KPʲVJR$ ;SGȟlWd|mEAC:4ũ{CqNaw(#?؂pgG9g2ƚ[84;'7s9*4 ^ī*61‰#j3 ?r;nm6f/-O^P_y3%y'7.J^tJR({8BǤܨ"/tN^e6KdUjK\\3TU]f,jiqWToh}iML-S9 us T74Ud\9W(;rPJ엜ATbЍ6if3/$.)l6@&<+N~F/{oOvcCGݮl2Yڄ7o5BPl/b+[{I?DD:\}.؆[ێˎDq Di􃼩,z^cy3K$ soZ2U2ʪ*?lx7ֿ05twV#'Ѫ'ȮzJTN_o W }4W#X&b{ gjݻI5}\sekg-:Hyl49RRCٯ>K}))LyfR {!$H"җ׵Uw  qwS)-=Ԟ4crCgFZ+1@)8aݗ{O2>ڡ&pW.tak3C{9FLdR ~jSO&KuQU,PQk=U !BW@pJWySL|GWL9 \kw_SJKKKKS:v ;PK6lv&Fmi_Uֽs~9ʪʖpJumޑ%hmOKKKKKw}r@! BR$ @%j꛵z3jى78 7]h@T9;Hja_pZ A~`ʾbj@xK>nҌb4–aiFwVKٔrsƫ?x@nZ6roY9ZcݕjcB{QSeEdoW =/vY1!"H&=X!rrio$-2cHt[s[B9698Ef4C#!N=h@b5T#G6Y͖j)d3>c{ uu JЄd +WGF%r܆&Pdhʵ'K]GEye D12,͊j1Q~˯}/y_3,8}iϷПVɔʒStwH(W¨m-ÄswBnZD3Xn|ZNioMm~5gVM*=u9!:ɷ Hul絅Wj#&/SqQ=c`j=azb(gHݡ;vUm?;H^wSSS8YȊ N58]w[3\dQ7E𹥉 sU;oM99tIrv9X䩓/wLJx䅥*Xל?e'>|ј(woє7"(ή͛WH}h_% T5ƨhoXS<4ڴ_LbcOol3ϥ7v8L{}+1΂zw8G0싖_Wz>]U?7r:5&7;د|e/o[y登z [r"'9H` (QcF#PF1ŔEBhIl)ka%Caԛ2#q1`:1'p./_,^}#V*6-S$0X6ԝBP+ H$C #11la @gy}zFB b@(#?՞frz;"Zqy/ߥքi0*y QUÈjv"%nF92gD5BBHm(5SY(0b/ ʔH'#7X+ƶ#[,(nl66*E\K3DMm=eAtfyϥ=Bg=b\"Y5W?բOE7AҴ)*42PSRh8tM92vP\||tÿ7uYi11x+Frвj070 =Y<):6>>&wztD_F(BzaUjs][^1U\0UGG-Dn ]- 0~_2 UIn#c-f 9\jZ3`Xօ.4Ѡ$%{ SHaI  KRSO'i"$.3^9TVVVVVwaڇ8=ݽR~ٹ<ԡx^[WZP07VVնut6TiL{DK֦ƀl>SZM?=ˮh4抬.cg[CyiuUYnzzzz~YR^oַחVeי'ο_\>ܷ,/73===Fkl(HOOʫm%S4'###==8+3#==Q,ޱ^gt5֬7$2D"jҤ\w_jkbrLZ5GEwb1?+!o敕]a_9Ҳ7>($fZ[L:j#4UNo׿m熭ʌe/o++6:+4PZw>#](zUݛeXUe%Q!ۡxbESbf>)BtB#^3fWYu=(6rغ[}q@go8Qus3nW~2old ySKUO${患߿ᄎ O?ͧ W j)6j ;}_?}<ѡWo~6R͍a/(^[*ۋO;2t:s[Q봍>,8O[Ǎ=_d4^WݮG;J+ӻ&vd뵦old:\jЅ-<9$2]Uio֦mu'w#ɵ[5I0 YQ]XujN"/uc1!wPCj9Nw5vvinfDX tftAg2d:]csGc7Awt92Zgы 7!)#~lR~-- m պ]MW qWPf}w}Dwv6TP߮&9zTKv7hW<ΉN2˫F-zQڴ) $Fm4ŠF "lFH!6W lh{B62pm@!X[#f1xdU_;`߼':?_kF8mG/kP*dle2W#s KBYYaڠ%C=MiZj8>݃KiŋAaҬ!Q^<;+;Z&N]5e.aUR*j[>@ȝ<5WW,t 9hkk5|}4%Ar%@7u_;]=ဖ#yEEΚ3g#q3~ɓ?.Y s7a/07UG͎qE4ت:\~t\ABfddMFruvN{)WѰ# ]ō4Mۮ0AF*/?B%8ŘyfM:?j83w5U.5:He27@tZP\]Y .͗:<&c_S.x+3 DbuOp0w~}JzӐ%O7- 8 r:$f^~d%zDP/ #Dwd!,@uȟ@_ O(DUNj-:SѢCђv 3 }$dsR)l?fXQ#P Z\Z@JQO:B zmé?>Q|"3{ ]8|Ӌj +4"yԢ[=J]LMZNiv1Z چC'k{f'/j:@Ukl_۞,gygN=qŴYgr=2!.<U_[XS?± $h)+RH%TP6PKs?|3<o7cJhu5LoBxE(7dRco>plxj[˿RXWioRG'9 Bdb$>=Of}aN*w=|tO?CS}}F*niv Y.!2bb;־tQ4sŘP33w}0[9ĊPHS4 u>n߾~/qĸֳ{R-sqe:N_2&ա̗ Cuǖ'ҜDS[~J_u2\Ph|+:hAV.qt͊BH(0)W֘;Hу~[wܹsK\H>ڼ#?}:РEOf]w׬ݸs΃g :4 q>J覒e؆L5G{wvӥNDs@B/$T;/҇zhCV vנq3gNʁ;,Ih41js6jx7s>=]’\RT3hxҁ"<.>~r\h|#2!G(U =6K))=|C}P'F͘;PںQ+>r ZO|Y+ݑ#AҲoNtǗe gv@Cg[b=bqw/b.}!;b}3 a۪UL-g^ ^$%cnD~6S,ۏP`DJX.EnqZbH",Ua躘@A\!)d/Vv>cB!BY'xq.O]ph_Ye;T^kW1~ԀSe@P/} ~*з7UqnjhoQt4UVUd0j vҾsโI޾R7\qt6cD-Rbن`Q1M .xI0"4VE`/*gW= (PWLm*~d_*GXc4,8F6llc: J},sIݸ[rc a/@Dх[c":ugNo2u¶?2 A7Mjk_CvǓ+j?^}XbrW,>2t^<{ǁ-":YĐx/BNv!Ya1%m!g8r3Rdn\f+JVؿ9hs\ nrT6 Dz(&z4w8k)Kh(apѯOmRlV/-b{uUA*O1+ƃy{{ǻYf-oΔ7u(o—~>Ɵ|HceiU_??WDoG}#LFCYEyڣz@I`6VCV Թ.D"z;Act_Ʃ@L}j$P|2W4|ȡ4/Vwz_,]ilY[IȘu2s r~+~vgDʇܳ)r!QD)[Z*W qTuE~񳩩[F'D)FCUizb|d_(l Fu|HB =kUGMit&žxJQ|$~qMD v 8PPza@ ze%4X]=Nb)i IPV'b*f*&IH3J0x8']|FODX`J;-e) ,u|@HB[`Ryh0r__U<.88D3ӆ-43bؘv@e7['j͢F 9 }m1FbFlQ71nrBxDZ ^5k0 6P1d#+\{@СXZ5;zg"IVS#Y+ s@g2ɥF ua⊏n?QVVVع4q_'2Gl\tY[y{G'Ǎ@`;^QQQQ~fk>Z!eK|@QA&هE*+**s/=G@qXjr?Y8)l"Ӓ/) K,CTlَd DL H \9{vZ6[m"`uː5ըz2a$2*k@([tO~ XF G5 PdlYMOP{,iG6~Q8=5 1 lP}yڂyoM+2m\C\ב4: rߢsi_[~vhwaQe!/I0XܒՇ#nv69 1ئʄj4Z`dwQIY Hd_ZXH@Cb."L9YlG עYݤSlAEJPa)fY$*Th#שM=B*e!36bS0-Ϝ+:@W6^\88XE O|3$ZQ[yy˺uG%6kwpʺOe;~3KR ~ɁA%ٕ8cK鹽{v Zj܆߳ڲ|'5~CEv9z?+i+Tfbo{& Nyvwถ¿*uEپM$@!ztQQ!t*IBzO%f珅e2m'?skιO;"0e+U^r E3U S>#JuErgTuE]`Y91Hѵw]hv0<||pW(UMP?[hU=#TzበƮ_7|x|>5>|?̓[6=h4?7ΆqnCK%n te7`CQNdB̔-gDN5]>N!4Mm7%Zd̒{:?in۝2ی]xnhȾqvLξ[!sN]QvNN `#6jJ]Rb?W6:K/i>gm<)z_[tKMi ډ95z+'jigg>4H vL.Ꜣ:_#bk&牼1`є?sNA1Yxg>0Z𴷆$ueZ(s ǧp_"ǶXE[?m^2MjecTsN~tS[O~}rkMڃM%'4n.~s~-zALiu㷍O=N>;X (IҩF>V6z 02{;_}ߋ%5$d}tPt]Cόa7JI`S >mlu_o+z[nz4KMпo6ڸibhgԆ'*='#=ore o]g~w bG>f\A {JVھ$x_?]b:oEs,>Y1Wuٲ_fڒz[SeSp\;V}{kt@_yb)UVQ{Էls>1ʳD&I%k|wZ1T[BԈo^a41i٣eX%'K-XvyN/Э_Һk>ŪGy=K"Kqy{܃$k/zgl@_0`tDVS"K*ćx\w-ZB}"?mdm;v_) bwfړkV\䡡1Rn߮3\jPg(VnF&8Q~k^er.5v]HU\^}m "ܞ! |#0vAI~ O&?8?/i.;}٧#@:MA3SlKB[ξrLfR϶2rvy? @=?@ +߹~%"ufC1thk =.WUgԌnђRłܫ׵C{$xEe7Z)qp]s J2a|Xo}f%/M3 ey6rm2fT|H7H6~ , zM EYpmwat{wX@tɃJj=>v2rtʂ)CI_x|_a"]f7]Zq-L=' Okc΄۹ YczpLlK:,Tj;|zZvl76g޽5:7"N2Rn)P 4睾G>|T4|SlfWX܋ɑ%;pKY*)i۷ku \QDd|Ѕ'ESD{Sҡ+REGO\ZEpP5;_~g[yAfXB-"#ƥxx"o`xypF_0½Gߔ(WgMYw&Π#XVmFlIm'EB|.@б[|hӒw(fϑfIC^"yJj4GWui~AQZڬrܾqRLu .-&K{]fg3Y`V]&3?xޓ /_\3[y^ӇvϏT=mސbJUm QͿoHrΎV7-ӥ)M={xC>]??-f}փ/MWn?Ǎ #f{g '0-{*us\9YrLO[CEn#ge$yPK 4^J%e?9Nޛ uON$sst+"9^2}E$ =}`wJ]({p-K$壔p p|;% m=&8,.h_/O(5 ?-OL]"B|E~<ݿ< 3Grd8ZnUDxN;-Kig #VԴ 05ԫ-%K$^1|ЍkT]/=$fQ( S0Fn1|n: ٙ|O&9R!gSӓ)ljYDvIχ)xZ]]Wȕg^S >AV#n[q!MF7vN#!d$[{xAKr糢z@ըWۅ0<¤Iͽq}R}x'z蕥-;wqf}Faׇˋ! 2oF oe}WoX$nn|>^uECB.PvZ^\^ 7zuo7bR VUZܐ__Ya|uR*qw>r*謁G)'ɼ<Ѵ!ۄz񁦺X$ /R%jj(z9wt8!t9(݄ۃ}_f_AOߦG5,aq7&,Xʰr !^j}NCx*D8@6h81A8t%4[Kvџ_SfB/U>º˞?bdRʲڨ.B.`d\/qr>E;{uRo@ӻqҖ`B-|޶e_W<*m]= y &uY?G(rά{SKI]|.&>45us_seG:JStze'j@E =Z1uzEhC&$+a]n"ȧ@rY"JZM+ۖ>[!> >|3Ӓ"~AV^Xe)`DZ~,!t l&ht_Xp^Q MzN۔xD_ ٠bMU,p$aFhk*4f8B7Ǎ Vg qs_avFQc!&]Zk|EՓR&8b34*ObiMEYԏ>2z뿾!Y@/I[՛m 4UrGhҠY^BsmE&XFI; 1N6At|M-i0/TCu:ix'BE,Un2U:h}'"[ؔMk B^\W.1Z/o"eAƌ3/f)J >nPPρ"Q_Q G uل( 1֥f_K^^tQK(:xn.nH_ˤ 0y6a65>י\*Kk+nSǷwϬGc7Yb aFՂpI<14%, T*ZJfb3 BVшD".P(zfs(+:5F defD"1jB( qW*f\.7LnnnfYRAE"Nx<OV)+y<@ p*r Ye CY>hZCYgVD"1 eu ¡3 :@T:=밌# t</UrđC?kyNgq\W[[P,j5xr^/JZN:;uzea, ꜑cg:Ν;oBY CQtƍO?E{A{ꅢ(EI&A;5k K,AQի!C8h4(5 BxyE/_!\r%/0##álFQtLE+ ֭[C e =BQ?nݺN:@P={6p޽t&$$`VQQɓbFV`0ֲTVRL&N6 ERaVݵkpΜ9(@SRRݴia ,@Qž={&$$@bvQEŋQvp(6448=z4ܹs(XB?(zرcQUe!q-(>|ðS(ZVV!LJJѣÇ(~'-[({n {=E?aXN:v!EQw߅ٳE͛7Cϟ 鉉rELaؑ#GP]v-a_5ׯ_0lРAekjjP3f ٳ(~}(={B8fFEAA__aڵkQ=rЩ,aIII(:|͛s2 fjdFqP`&v]~}!:2Ά 6OrnS]0H3}D|EerRi61f攻Bz^MKHz0KX 4~HXyy+$qS*NV0qcD&@<ȼ(Yݺ q]󈽂=!f=}A&QbcWLG<хv;FFrA]|ɚQ;7:*nZr߹l_poǞx~zӪ~x~Ͽ>=+}=Hlg '~PtЩ >1ċ5;NCNp'Tu P2դ }'w/>x|8).>uiy>1čX}_X=CI)2c#.]7t%ȱNؤ9'~z'.>.1E_v+,y]۳mӮEָ!>f}ĉOʬhK?!/k농;(FPڵۏުPiV{uPQ¦k,ܤBWT$g_rQ5i6'HviaS1kVUkd2X_y<}8wL&`O:&Yƥ5ʮyy "gC;d2YHL[:)Ø6f, -OP)ʽVStce!CZUv_ͥ=2sxtfϿ*PXC6zz i+YRYxFD?:Dq~V>y=Gn x}`( ̵c: +]e'$,.a ac%()^{o*+߂CG!MNblOv+hݾ_egaR/זs^Q^6T!oBhBÍݚdכmg ȹqgǁ)A>m:9 %:/};'#+lS}O@y֍;L(u y|^U߰H7;{~H9|)aPN/%Ħ1$Z&Zfk@+*5cv*Ύ p`]:s`AWvxkt@OZL71XXTn^򒧮Vnq7dT|TkŤ4/{MMqzs 96uAry i .:}*o!>ufU*Fr/yR&oxq aC<ڿ[ |;:np`ӽ-{qwlmYсe ]-_,7qꐖ>w [;u.M\i7򹱹q !z3".0Oa3\^%zIDJߨ>ށAÎ=-#e{f_s7ˌ0ѣB%  !N8U>~v9 9gΖ"#ҩzwRi{-zEzy]H/C!Ds0F +̒$q#zE%%}3)61e5c2ƥX9 -۬>Qx;{ڸqsޝֵuInd#ڭ5%Gg3r5iE $W=<2 :|VZ ǗO]˫ʧn*=E(*v)ݬH]"d6G ;M_avգcks kis>[joojNghk۵h=Ř{_wlzL7^y鞲Awo?9\yШڿ5dS'j+|L~?̧ߌ _TSm枴>~z.S>)uP`Bw|=gl!}ۄSٸ_'3g*%QA<B_L @i=n2ⱋQc:IjA8a8:. _o/?xpҨۅJBUe@2*|ZKlvv=j=zt.z/t3 L'oEe}t2 iKfw /T aAe5QB>ӭ=)}6( Oxf:km[y !DG'TSbn1eF 3ui7^N`I0]S#.!lCTu͜y.-kX<0x/ &uѣٷ8]dMIeB IM~P9Ƒ\:M[ɄQmyc+?Xv/*̟5eLY \t~}ilPdj.y,L$cԀނh4Rz1zx{#vIJ׆QSl]yι5)/[yS' +ӝ"Oӧ 3BGB_Z{aƻWkA|.J&l>$La؀He7\3`HO OMiJ_Aƒd0  Ʌ`Z|D(q/0D` k?-8dqS>12V@&2.@ˋEuwʲw KOU1ψ_!@[ s*u2_fʝFvY,so:K Oa^",Ԧ'\>(4W=_|"ohGk;Hjk^vХMkf*+MK k?\տ4VZc&NL<*)^YB"ao__ym5̝G NYLǿ~9,ŗ*n͋|j"ZU2?3gfDSv_U7oxmStt^\6 w0 >)V<ͩ  AC a˖-_<<|Uş6<4{Hx#(a#eXR6\76 F~`m!'\-O0,BՕk'1Lm&::{:>Q}Dv |LN_w ;{ܩ-'XzuMYbX)E[/|hRo7N^VݦSBkv[];$K==x\xo_րNs6Y]&efEpsnKXֺ2kB{.2O. F(S1M#e7}ԌJyZ33jA'bcտ{d\"<@FWH oJ[ {Kv c&[KYgW "6Fr\$0>>=)S xx&KS! esdk{%": \qN4 9 q$W.#V(dȔ!ߟWp"Qnb&:2"³CH33{EٌЉeو0y1Vo,19e.D`v-eu׿m 2^G3ˆ bwKX=[x?\:^0OˋT2wh4VRrp+}ӎla2꧷TN[j ٥}L@\qR!@RTTM zZn1{eED.?pqM$n*]"s ( `o7n PV8naD{Ta4 wn9d٣7$#5f3LF1d2Ylvd6[lvf1f9S/pmޓ׊FcOM~AȰh۳rT)x+Nxt?B iBV$ǟ+ fM&f&Ŋ&t5<.)5o_w[?}|h4y ~Ϧs-5000g^_LxwݘwB/WVݝs?rÅ<=ݿ:TZc0S/ZOr/BTIKOf).4e²=bGYAuYTWN20000jFg=KDDO۝WTێf8OF̍Ht: $1۱ϒS̊1rQNbYU{ΒmF~^-Tg 4000SyZ İ?Fvj}MYGM Hugņ0Qz|ێfN\Us{5?/H$l#C|EkVc]ͣ'DMşL0º_;4]U'+nk}^s靅(6>"!+y{1c~ FvboZ ;t|n.Ճ)@DŒa)Ҵ0r3KpyIlu\9{nհ& NaV5 ^/yzt)el8.9$\>" ҡ̐'ltAI'CҎ2Z|i):5FwWsZ0$2HOYɃO-X4,k" ڲJP#DHԽe5_ܽjQ_]ݚ@M9Wn 6H}pɀ":?-$;z}CwsaY~nKYYOXp4K# ڷ0LG8!rU8] %GzSG[eʲ@46a-rTreaf rܛ64DY,Ih)Je#|P}ގ04w}MօlFN~Hz\L 7RR%X"̍Z8vѾ_r) IleX5l$SZM\سc|u N/ˈ7eow;N :z| K 6~uh0ħFkYrN82WҷY Ϯ])g52Z)Tw⢢͌'E DwG[Ev9"-J,6eDAt?ANY _ pA 3CKL3 6bLpA0 #b֌}Ȍu$7ib ͵Ԗd?~\aԻ] _)L%zIx\ө+fbW>7Y8bψv=R9n<,R7n5yy3ו|ub%gnǥs$dmױ'D<Y )Zo\7C(Q3 'JiA AS?~i+:A{@@P՚_fҔ9WE֚ݿbK G& `FDqa xb$yub{g"˴}@Y]_ǠC^șH kM4;>y:8AH z]tIMdgfE+wc k ? KGُ3+.Y *mjCd6l;uɏ#L 7|5Uk2B샤g A`=R'~*+*2. '6}@e6!neZazvo>ՖWjHlO97Mq6[|{7K'NȥGGl54;?xD+_N{ bß}z)峅ѵW;F \m(w exg)*K}|p?]?<=o5Uh'-\>( `{ϖÆuqe痹'F۬ 珈 zmk(ln. A| <47^" Aɩ{Ϲ3וW*=‚dnٶ釖 pСE$^Q*6R, xxsx{ O[fpzKI[@A|Vħ7//9{1yȥgׂ9|/'q9b}}FY|{YZʙk[_I#ua] yav )ƥ|;222r;Y^vS~Z@_O!~iA Q7^yJ}I9t!/M yޑd106$8҉Af#"x3v+ry;lΓƂg4<}\}tӿOSRvAQR[is.T iܨ.kNkcM@l_YYeSC5tD?0` ,{pIp~O16kQ"` zm|}m CrjqbVRo ^W66LS[Ἳg)'r֗Z{ ϛ yOB!St/6^{3K;:/Pl46!M|Ih5(\ ˇf AĠr\3u8) e4N_72M.(wA鉵ן=ȀD0(1PZ P\qǫKՠ:7!5Eϋ ^QR\6^r >A+!nc來%C'BBČ,i9"4K"MF,6' \Jsٖ)s ZZS`p O%tKy| Xv;Xj/"=U"l{U?gfKڦTB5EU+^EQT*ظW,( (EtBI(!$6ew3?6,'3sN6<3w>msv:PY蜡В׃}'Ǒ3^T<16bքj<]W_?2Ii6@162L̓':{\zw~=[XXXX>Bt+Gpua mSHJ@MM!) NS!}Rv]9z o޼yb˓U^_YJٹCSp,;f~V?3_.|5\|r7G^D-OĒZr3ftOeU6BEf 6:Sb/!Mo-.^8B h}c˖-{᧛k?yq9XB^Nt{Fn)Q8`:XgKG3ZĻ~/!u+=UW[ {MgoBpo\ITųyê7p̹{֞ybx態FE}ST ;*|͘ sX" V/`H]F9S #y;u^Z};LMU7˯CR6n3hgU3m#7+2ő'LKݸ~ᘿ!Jo°!rcA~i@rL8zGm0J֚HP{Y]I'ItLGdȂbSˊ\CR1̣iODPqW' ^K߁ReϚ_a6W0/J }E֪+^2FܱwGlԀi.A/k)=s[GOu$ZӒy)Zg~ }dFbhzʑS"25~e'uvT,iK+Lwjis4"”{WkG6LvZVIw7wz3x߼=[xރƯH v|Ʋ qďc@$ IL멼Rz+$UG*sN>S$U<4rGIFI6}1iAze(~U]6V[_[]{#''''' /aQJIhl9\>ʨcGw_N_aӉe9gϝi \]3jmUz}T*8PַQJ)p5V^;/#_/:|$'''¢}e7ACGɅڼڠ JPflײ[qzJYQZHb OGaHS)Y>s˶]^jQ٫soXOLO\عLN3hCK'J>q)x,ҍ> 'SqOMOXܺ՜怑ϭy,ß<+UIs2?2s`=+/҇֡12^FB<ҳ/#kF-7;q&I.=z{ x# V /19wֻ}a{õ^SN30W8tǻ坞UG^QؑOy\K_IE?Z6 y_Gia+e-q:+e2?!8S7DfOCH1xb3 B$z'C,68,[뛫z凫ڗkw y $NIgs-]7K{nO~' ]AL E"@@QCE EbQ*x ݄=3HrRޙw[;nΤC޻ȮwySؘqX8O|lrzdTTfο.@'QQQQQ-:)]Sm q1 ұ\Kɉ>wwZAtTTTTٽf;7 8rjU]p~Aנ鮙AK]ޏWgQswȵ7v@ho6X e70>f](U˅EAQjp VU|&NvVTիN]L@TZ[2qG@_DBB]6ckcSoF+vnU78H\&me3uB9l|jb!;݉ȽR$U8C`?ʙWH ׆s~6~G۫u$ DR'k0KKƙA!(ݶy1?KغUw<=ZC^w>.Ϭ1xmMEq[GCĽE,Ahmʧ~mݸ }+hw58;lڻqJxc6&ȱ FMOy5 MUgw~ خ ~zk xg a5単:7wkΐ=Ol9SOK%֝Xi^1>vWE/j|mH,:>W˚N}ޔBLK;rDڹ#@#FO\ȯ~6b@&QK]gOZΞ0QPY[r@Qs~5^ƔgR)߯\S4me+@o;-r('p[OɮU OdظWor:/Td~᯺بʋjUybv^M.S_o.-;7e ;_A[3vQCqyemMf;}n M36SٲP蝉 ~rѼ݇vr^' ѽa~u4?Me_tW<ĕMVI<0彗96֯HVKk1sLP>`xrV'Јؚ,\);Xf(Taa /)RXm'6JyB,6JCK`VN^Q#b+- ,DO`YTv^ݹtڳXuG=zb^YTv뙬3,43trcvG~yyLxzQu۷?IYɉc_U9hZc?d[#!-7O-)W`/:f߽ c޸ڴ$u=cEά=Hot*jM M1|6xr $Q~G2Kѹ<!tM dG69l+^Bŷ\ B\X./*@#0]̬ή~0i=W_?kˑ?f(5I"7;xzΝ=C_r;[zy L`Zskuta޶7>x3Wo-{^!e..5.#F(D+4Hb, g8]N^+Â(H, ;A6b so' 'g4ɡ1|ŀqRD Sš:m>(_7xOLҨ@MdMEwyzyOڲabo4c6>}]8uw& $7]y t\ر*=v;M&`Ƞxrvju)#H>ĞB7t^%ut5] OW7ߠ9"$ j6f}F̙C_ 6|wn{㒷sLb0C&$3yzrp@K jҦcw|ek0Cn׳7i+8rK0:SsHBG Œޏ$ _<ٖ"F3zTyéѣiBgGXw*#Bˏ+ꄡ,X8VvZ~sl ٚ8W0S~g3H!遗xʓ{xfWp\囫@ aH̓H5?'bڼj??@HEa(sOOϴM') @E"B\xb[IT]]y!ȴ j10lUM>'K(o+7ł–Bڞ8n_ ZjJXUcRz|ErB[3 l׷@Kc+д` 0W^L] } oӟ.*/Š+̙7'V儤=(az/Jc$b-H䰗;" <2@)Sa2:RTrg9ނp[EMO$,hzT "Et^;76QЏdHqgr:5L.]QE!q~'^b.i D#v9ljw(pYQ@nSp2逕'wu _`7/]nN(/| ċÆ$Jn }c׬AAvm{{==>PNă@GS^&bb_P(J=zʫ#0T1 Ox=@GegE]W[V QgsGIU{Us}ַ?n~j20\n+hnrw<6 o}Qb)DϢU.3s( º̯;]Y|Xa{=hʢax,:wjRBdG%\s|qjѮ?^j?njuAVHVgYձrؘ8d '3v]c^7]ckƢiA7q4#<71>Gt1>])l"6AI.SR4L>'(&Z%J KK W1IRH }#*`L*ScG{hΰXTh|&*Mxt||m O es T׬#BqC'H O)t5L:w."'tM"|R P@ Wi{!ʠ`T"OIJТ.\Np;AkKTɣ3yhvt&VT~յR;"¨#:ϕcqyj/RTG߿xN5ws`<;zpnJ.AH> ~KϮe3^nj< v~p8:޿Z7_ϑ*.W4-\3FΏζ+ïis]馸.N}mDқ B^ȉ q.)iBd)n!Ǘ- EJ5E&ũ 46ib%{ö`NE䑃]\ o>p͙cUj??*ɃҪB EQP{׾.䣃#anWQ~^Mue>JнE~*]ƺAS?X}3GJG8$rI 6Wqm9 !Hpw#]wڭ֪7`IMd1zKяL܈uOy Wu;[R[Uy@@$zKqT5pA" Sģ`_j!^a !:B"NdΩ'Pjn>>Ց[-r#737T?rPH$SYxRStPVt)5upi{=ɽwXp[kD;0^wy@l%8x]#&/\٠@ W}-V?I t3$R=El8;)9aV'6&/Us ' O4biO՘Kp!b#@g4$C; "J+ PJ!@tw( GhQ 9 {'zG^-EQu9]25@h.r,ʘ=vmCvdPRA#?EQ'^>+fjW .9鉁!0Mz6x.asg'9ѹ` Tll/m) l\Uh魵\ӇNZ?<^s*kQ??nB|d V3bCH7%"% q <-H[Ma`Dl(

K]?YlF>a <2A,TQAo @!@Jp_y:mNʈ#_`썉\Q[.H/!ӖXM9fxQӭ-w}ozن%݂8 nSۧʅ pXzGܛ"S`{wrma_Sػ#P\e#/bEY®o<=D#,xv wA#v";gI`kpiڎ[l?WÃpZmS ) 5g|K&/xX 短 ;\aF\hWeAtre(EI\/:\| ^FѸ!%Kn\'IG?'(\%| '>9 *b)*mQ'$K7 ɰ1BD%^|Zte%E IT\m皢(45Vjm(:.@NHx9WWXԩi.lM7s-.w|`-QN[/i1Ǫt7UUPh"R|8 NW0pɞgoT4J (6<[bTE$O  `Qmw>d#(`5TW&$QAr!io\.jJ ?>)ܞxwg\,2xJ#bl[O5ŏEWڐgK{iFG(axY'Ҁg,W/%kf){9irA0-$.@ .]ko6\7p~"$.z(hWW@M6' 87\a\qA1t|"&pU0vu?M݀# mZsP>0?u6ap5 #cTe֠O4RR{)Ǡ4\/3 iŠq0k X H kCujYQ7ڃ̜/T$6Si/+$a!dg_.7:z:GD(ڊSs `:,H"4qg)iy<`j T&\nۻԷH\dYWD}fiY[SR/J t%iy ^6:Keb \|Cظ$xl^{A=}>u;U[f(9vR`w̋['p-ǮK5eZP7.A{ C|߶?[N'ġמ:[iǃBlמ_lRT}I7{~խq}cuǷ5/@|)x]MF$y@^% =Yh.<5OAv2(I[ڊ]-Uдc7^+--lV}A4边âT20|6Qtu\WkҰ0_}!roj2VuRDm=#~1̅ffJFS鞏_6hdO{s^ᢺǟ{po?!% JO2헹G7l2'Q$ Ez\'{jqJK3r Ѱ( /74`'W '2~i?i/RXk(jnХnY꛾`i:;LRSm"K b,foNn1>a~~&F57Zar+5=x }!BjW) tuYEO@r7;=3x`#l d\@TRD$ tfԇVtb J W}F`x&E; 썷d.Z"TM |o#Di1_??XL?]Ѭqre6j ԇ{Gƅ3qK5\-7~zF=|e؁˵?丂vnr1_9}EdMeQ%c,馴>2#GKߪm4w8.cm)oDxxsP7n/-o5E.LjukT۶}yq-w?͢]]|dr{_jYm/=YX%S+H|:;/7<<٣(LgY2z?_׷Y }52lhkmh-zbſ^ـQ3DS{?ArqKKpxmfcQUk064קHx!?յkPddn4e !D2߀Psjff>bAE)Q32! b$*BLׯ&UŻ׬ˎyQqrxb":)CX :-LgQNo#eу }ϙcJB|^#y&z' yZB04EWVHbB+ˮGҳB88c7gUaب3'*P#$0uB63؟H89[xCvah! !xC,m.CJ TJ(t@$+J?_&hBTV b46<½ڦBzC.--[?\}TvpXAc}0W遬rT6jtи@1}Nkj:__12AF}15T##zdž@_Qܘ:qDD8;4nZ }("RK8V{ZPe׫*uU{!] o-~)bB#DDV9rP( T:j[fQ $&5W bH H8;q"_.C9w%j+KM 7V9;WGMZ[е,pm/s}s@3/ySd$>tSPUiNv]. 7kU>*%EQ5eg ^_W?!*aLgҒ%ʦPLJQjl,$jݘo\}dƍW W7F&vrQ*^J*'jn䶶GKu rAUi^/S6(UciijuH z翎p8pk CǍN 2O,_#;Z-,{D$7}xV?<1Up:Up\6rE`"(2HӃE[jy5wPW~?Y;}kbJ_ 5r)B2zK 6lI+.,ݞ')4VG ~YF}vJA#V}:12T2nlRΖ\/ z3-*cm!c1X l_py5PlM]cLk[m+{%BD>h5/ag1/O{aۿ30I}Ce[Y DŎ7,ȧP A `ѭ<[6&}}U8H%[젦K}nP+>žm3Ae맍28>ĔT_k#cN~v\@ʯ\_u1r!h 5Q#Ey.[|R@suIkPWYe^AiB?yqkq(R yG'*(%W$r?~~iO~x#mj퟈@XJKEDBxX mQw]YRج|졠o];ro0(e/ܓ h2QpH D3 eJQɿF W5961wlػw ; 7o|?U9jœR5?ł@^+cCߐBhYc@( Z {Z ST6%$=-/p$>ڈC -r$I4kPt9MUV2u`hxBḒM i@ttJ" 0VcS֢ V mZU HGcd!6"Tei147ֵo S :2$@.]WY۽a߮!2؁T,& xZUcͭ:CۚuVo@RJ9uʘ _2%z? pZ[Z V8ZkpZ*8 uBlr9RmTQV_ߤ2E$J\eUd!Ro)V6w\UxB\SjBߠ@Nrc Xr[ @RIxZLZ̥'Jj·[mĪJ&-e5B&:1p݉zɣqgxlzne⇖e`\H_1-{޻"F1S$w]4 xe= /Qf4\owNg"͕{[⁀;le ~$&#`"ؓl1[Md1QKFKceJbxrXYs[ʣܳo#dڎHɏ_>>.K9LfZ @,LvX, u:[an7 BXl6E"P(fY.Z[[vT*u8zB(Hfh B-lr[rI$& E"Q[[dJj"V(X.H(th,[mX)l6X6B6 0bmH$2 &I&QJncX0bG1͌X/Hl6[[[۳cbJJ$6V  0nfe2 R3 B&z1h4zx1" cz{d,lϺnzpu:0X.naxκ cH[La6֭YwζdzBlc≠Yxwܞe V<с(u S% x> 2xq '~\#lyDkaVqq4w’z!iyhʂ=iU__O ٻw/M|0:M'N`fڴi4M gΜ0̱ch~7yh޿?p޼y4M766vǍ!<Ÿ/Bϻem,0uuu4M/\c~!\f M'ON:m^w !?i~뭷<8pas4jƎ?as4/3 i&w1B2l0a>S-[4uVa{1 q0Lqq1Mӏ<0ncKs=Gtvv6pԨQnckkkBCG}0SN1 3eM&YfA=6B~z<!tdh0amի!7nt0̒%KhdclNNMӫVbm0ʕ+=0O> O4Mo޼a.]bfȑ c-Z!ܽ{^{ӧO{5:{cuyw=Ι3f1633W^yc] /<|pիWB+7n0 ӿ}B._Bm6>,Mӗ/_fSCkã*n˦lv6 )dS)JhJ*"("  " В@Bz/em?ٙ7 =8ws~w]Bv&g;~,pȑJUVw8k4Ϝ9T*?sN2&L@1bhLLL49+ʃ-**g,Yb4gF JGPg333J/lrv˖-FqҥB !,..ph4:tHT[իWFc\\!OpB_(ӧOC;ikkS*qqq&g?uLΘ1CT޹sGT.]B]|R!hi:cRkJHm.gF `ydPay&%t!$>$Z' Q6z:qSByl%lw͑.%1d{p4]7jCCU8I*ynn mYARk>4KnB"gEsFVȠ@Y&џd0BQ}.]ZozNrÓ[F1dlP=~/TBC?w\k5`xH sK|jRĦ?! s"Blʡ@d(.$RIaL$ =ƒU ¦j'JJRc<`Ʀ o8M]FQ4gǾ>BC:ރ1+GuӜ" ;r>7Ij˃}g2[^\Ie߫?|A/L`@ԻG~>nw%37iXc9CmYTa<qFKZ"TGZ7yj7z1BF{e?0:P5Hu@d6g>q6 /$I_|4|W] fP+ 5gNʸY="knÉec;)5aY0Sw|VQu`q7=5n(rT yOG1ǰʼnL:U+c۝ i9IVNx\um+ 8nԠ? )ŭb[SjBRK)ox>?qb>uW_m:uDCΝ?O-mO9IVs%S>K=nlS]~ NזxVvM}}XvD6p_>>;*zy;ƽ #bB"Čߡ3.!dhɈpd!5|"FLz/`!y@Oȑ a9Fuٻ%nƶo&,c#WԠ[6Q0iV#~,LmȩֵxuW8>vregpRҷ ~jKcOBI 6x5ԏwrCg\ o}ʛrnm ZW'J 4aTOFSybۖRmcN>XjAm]ڙ[?^rS7}#s us0ٴ}D=!V_:}Qos$!ԜNKT{wdlńًy #1u w/ed"GRHHHXl$fB˭L9!B9<ʭ5U߹|ؗxZ#  tM&OCa ҉";^I}v~ÆˬjRV]|kرcgh6kR?wρG =I^LaFc!|P͌CbM|?gr$b Cdi7s´I zU\4r!{hzpF1n˜1~3/HMj$zXiS&1JLN3ns$eյEw邰K&  1禩O6i҄&Q=LaF dŐ<P6m--Ը&RSI2t~h1ƨ^`hғ1ZLKSq̍tOW>b0?o ]݌޽[)@֮է:Y5MDa,NJuE]]ynw -Ǜ)rlv ݇]hs$2Tg@KK /,RUV]f0$'_R#{9IZԍx8_`5/l-yInK}IV mє_)cCZ Q)" #;p)fmMW'B5yn綄Nníנ`>Xa V[3mU>pa6Y \EPo_Y5ǚ)9㇤VoOV|=b`a{NšNlR=0 #Ϥ"5UE9 1m&hYNhh)|iw9 ȤR5ab_\ ]JoW&'ewS@UAFZe8rTe;:q)ͽc 1k0 Z"s_+uuʢ߈+ ^}=mmw*[v2VY9Q"ڋk`ܤ>+*c3RWu8TlZ}p,&ER%9p|mɺz\ԍlMⶍw^mm+'|=Ȼ|TiogyjF^"{y. hwyZ! 9ikU[x'ߞ8惬1ʳe9ښkKz А~/Q" nXɻszI]]Ga}ztu*[TWoM`؞2ߠo~1dr-X7y=`^f.+ny)@~MdgŃujUH,z%B@Anv!"+W{WcEcFjUQZ !~zOSw? ZM~AJ(s8{?"O+0UC M<3=S u lEe;v@*~𯳞_#uU_ر9M=ed-A EV2~ G&$tݽ3~@)%DY;FPZTeE>.`0 Fh,;oزsG/A\}oUo7mp(m^c%qKDuL7!MJ]3S]Vkk-wAsSZ$NnO^hFm:'/~d;!0%V ; NQGEWG w`Зv"B]bȁm$TU ||0r"Ər<⅏?h=G/I^kfQ̞ ># 0EmpPQP[[Vv=ںv^~,mjtƺɺV*fBMgIc "Yx$V jJ$Y|D\Y|xȐχ BMLc0ZZsj|H[|ANvϽC?76[Y/r+WxasWk;t\.GO ɉ{0aʊ&>ؓmgpcP*(V|>c4U e)S7D.3Wh~x/[d'fPR\h;,"X<{ό/ %c|Uqh\FS&KΑ BSUuU.PD|q U[#O 3rTlezA|6rQolmY=+{F()wW'm)ZK$^*9d+Q($vUV{y/{*c_\sl碟a/g&?_4GsΞ߯j&]@D,ߋ\z -[w[ճa ͑`~/NВIGt혡.\2/-b޺5zBm}iΝ8;\BcK-Ku tV=$-C-+{ygrloä>sQ֨;7~]B#XD%ѡ^cT/ϐ'lM_a*oaQKO@ (#l l!QY %I%FdC )I9b9$ryЀy @z ˦QA^U/h%b`~ę:^zH[g|G*x@{Cyi ̳b]kk_tP}W'gS"U> !csvya?-vɿFu iWmήPru_R{,زxl8h0x7FE}_9R#BՀ"5HTGH  [ {"yRy1V,9-tTK9J1M.kT$r}@DTh>iQAG:q@X@Հo7BhPm]&M ɵi !}hDc@RL0L"8x,XIJL~pr˗/?qsל(o|dc['Z 8:3_[Ҧ [.q.uCaV̓ 1zx+/-iCsXS[m]7zr+N5Jr/ sw,׀=H9-dl`O,3&3M<2]9L$FO .iX0ld>s̰ØS*:x.L62ƼK>[ RۓI-مQcC!gA<[/%-<`{35CнHHT00ZȞFuJ[ŬS˖YlFG63lYK$ 0)y|`*V) Baȑb]b#W<3dVCylS 5G#rh IvHɼ'P &lH$I *9}~gvxH䎌0# B0m7]2rUڔAJYrmU3#GŇ{Hw^^|iٴ甝t %Uu"c l{Z-o)<\'K%Ée,@ldUEƚ9OŨ U  iHE`O1&UĚL&쒴Id!Ũi+2ve" Ek,T[a6ԅk) rդo:JlTlQn7HXHقEdK#2OjGz*Ycx jHol}5<#{Yuk+I[ݢ)|\|O_ ŏ(!l(JI2䟸<"W"`2 4&d#kK#Ӄ)%a3&H.Qa.wV]XbcqF;G[7n%llP !qT":,Á.\H|TH@w!K(&=M, !`aPe8foI]Zp!&y \;ܓO 벢ȹY88of k$!@e(gg蹤.% X sd ٟUl`y, KlE4H;sXGxj's]i@}SޞlT޶^=Sy(Vi0Ϥ1a}խXvQc~I}T@M`.,$j}fc>?   r GOlG3 lH:gtYg;~W!μѱ/mSB3Jޒt\%BCIK4Pu +٤ LEܻւ?U$Ч f~y* F&d {D-˴o.PXqn'C^|NA.!~bb)Rpc/L}oa@7?;dȐacߒ@:Ժccccc'ߙMfa F%EYba;3d+ܫKkޙA Nz@& f:0װ5LHa$0o:q{mR/6+7juƦs &vJyҵf:111LFg<9ii?٦5"s7WEaTd-j#Pi20 F\pl4ȮC.܋ᓻmQV-rه{9'[p %Z(`byR]A<ע=ػ8Ys跄'Oݢ-8;8Ma]smNL~yl={Ǿ*F&G%L''!̽_;o>hc7unr˫p-E>mj1|}^uB<~`sCҗ1Ȗ*/O7v6fnxTz /NX%\~ϟnne :Jd|M_ _pњdY J"Fw5ra{#?]Ja:ZL +/U /߾ꝏΉV(K6*lSG]~޺!/-ғF) "|ɵٶnUK& [q@-j`/ bƏ @s^(ƽāg/t淆DN>Z[~ m!hHyB=yٞ;uݪ;~e bsK-D{DM>VTBg`Hа!r[V[I~6нᩆ%+Z[ψ7XwhxM?0lXp]M} P_vE߇}}Ƌ诅>|31 2@TݠB^xdhۤV5+Rݾ|ވU(^+Р:B3~G[K6>>zswvhWoբQ{*n6>G>CՓT,Z:s#C 9{J٣^|֏L^?y)(x#W\m[AlB  uw;6S A[ʘjDq l/]z!4LzuNlXXXX?O'k@!C}$;F1yT޸T9z[Epd߰Сږ6sFnu. GB*>"@aZғqY d㷤"+Z6i۷l/S_YówM݉ff߻~|զߝ9h%#j_~ڽ5kkQo_wC3 ^WM~x`QSٹ]]+OYnX\ au+VvG[NmfyyQNLt$`=i5P1IOAW C dɛCbbLܚ}R14.wݽ|!xҢ*؜z?r  LO9$tC?0 ZнAXއhn9^Gnv&Ξ-&-qr$Af?6Mz G{e_a^\դ'_=D778ɀ  ?radog41%R2+*R.?z>v玾5Wx/Nd'\='LjNuwυ/ zo,Ӓ\B KHyae_@UV{Ce2ro;U`xHr:o,Oi@Pus?|u篖Gm'M[A̽׀7u**iM$L'7ITFaj[ Vi/.x{٤0tsYʢ'GX[wl,3475 .~~/ϞMbUqصkWL6"?*л><KEGxI!ZUͽX\?5α=kދJA=BU%?y)% rśdc'1*C'kCE,͔|"H@T cdrHk*:ܓ+:;{x7'g\wvr1 S\ Uw~%Je)i}u3u:pfh| 㓤Zda&j@gggI_ɮ9f}v_];0ڠi,i5ïTȝ_[N{Np2c:M~aQs3%Φ/ Д4ɇǛdR!4?0gV]j.ږ?hsp@F* 4ueXpƊ@a֖jܰ|DsK,MQ[x`w$x=FOT_Kl5<ɴA6IhyLjRGKSvM=ؽdcO@KiI 44\߱H =V jRE.Ol/w6MS}Uar?qmcA";&k 6(fKTz#]*뗏1>>0.Pը < vA!8<6g-;~b>@C{mVNAhuqjiаiW^v"0?;MףZeɸQBuU5vW7gO>~V?(|x<wh7,7B#0X1I 692O^F*(037Rѫn֖^wζS[ ]B؞@#t,2n<6N(-*tH w1$mQY"RaS5`A`l \Z AN {<'-׿bӴ'rmoӻݽEw/6C_u|?>_]:u$7!lW@9<&o s3k Y^ӯ93**jت{^S_^<,$O;|P4^66V߾䯐?v緵úԻN"]edC,Ibmê7k#VUPRflST5K)c 1hkFW|!r6kCyF`#y] u &G)ŠKX<2mMi|k$ǂ uCs&uswƮ;iY-l5t.JBǎAQ;10O @Ǟr2 0,ԣ!GR. ^V$R:AXZ9HR5b |Ms*KD2)2BPk/O?q EN&nNR mu  Ӕ6E){ȤF~oʠt 7f>HJ^1ݪ8I"lôf1S JCl 9agВVȮIjl#k: n[AP.fgyV+[@7`d]`-ax<^>^ ? {P_g7 Ogny3_ JEdi?Ѧ8#N /Z|w8ۥ3z;EM*kuUg:1d2^>_oIrRs]2_}= .(u0LÔ{3Z2!ЩK4Fw7gA,8y("T"ԄOOSs |[CL Q]_ ASsÅ/']P۬V79{pa͚q C4jZ "Q ;U`a3X1mvlك <*j2uwmTb0m-uFUƭĻb>y9c_-\zTwJ@[♣6=^@kз՗Oy qϋ_56O.IF-.ZemO\Xs|ء)au^ })M 0 t%3o'޲ Yߺtbo% z#!5/}IPdb.,edl⿺;k5M\]e/~Aǚs}yqՕ+? sD 0wpvW\qeZd792Adt<5)%Teņ~]ŭu%iHDxOw^\x7Raq=S#  v p6wVl7Q[}M(x)Cziv^sãƅlCfbƊy%]'-}/ec"W{Xytߕ,~ss@2P|IIII1ÃdrM2FܽO]c^_Mo((DXCy-IؔA>2_vѤf i}VpuũS<{}k-=XT{vX,*XՠłIc0"V-m灤VQ/߫c|ULZJ.U:>V~r?t2zx70Q is2u-~2hQ}%S4G z ̳slD2?!n$]8 jE.I>ż =bD6`n>i$-$9`PVHKĚ\j:QzZG Gs5Ć{-5INohͺqA݈DtINJUk'CM3$wߪA̜%^EIs]f8![E]6r (~Tĝu֮w;ǿ1k֬(t6Q{{ ǿo '^-%_ɹ5XSn%zj-$ U`,yܝgK8diAKlor?[@Ϩɖ]OqMEl-V_r(aA-){y1QUx6Ae㑚}[a-HTI@CdBI1@MbL`> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 518>> stream xA 0 =" ʝ<2;EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$) endstream endobj 362 0 obj << /Type /XObject /Subtype /Image /Width 600 /Height 196 /SMask 361 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 600 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 79658>> stream x}gxT:3)LzޛzG,(\Ć]{-XbWP5*Bzg2ICȰ>{L>C}^'yy8== ӏd6 Űb0VÙU0fߩ_c+$BPCOrt0$۶v-A@+}PCܲE0`w?B~VH:b#EYBQ B٨0 O%Ǝ"Ӧf#tVH"VA"$2fW3}>rS#;>]1)/%Eɟ"aq/mm\1ݱCU"H,+0]jZ>KU ()&%#tyLUs $cE1GV$ŋT0P`QOOF!l9ΈPV1ɐ(dFi@71U(2Pը Bjb H*&1D,Oj+F:]9ǜK ~EPquAYQazYK]O!) U wF5$^BAN OR b䉩V *TdD5,@؀=<(^.Ȳ"7EbBHnSjЊBdU,j9n#<B* &AL #eƧtK fQ<cB*wF@9*`~DP";i#DQV<[j `bH!Bs0 Ȟ'Mo>1- )E$O6U"86qiU}⦮)˒ i|B>dֽz_ȟ(s4PNޢmx0V-b*&K&$[KMa$d"IL8f71LFV a:ɸ峒\V4/+S *VPdG62 ; Y#@"ܦbX 3=64-v{BY Y] ۆ[xl0ԏ F=LY'R6<<&Ԏ YGWͱ=jr `?>ݵM io ϖQSgN%Rd#= _G,.Gyz v *~[3*̅' Y!hX݉9y{8D’D楺HPALŬ#!3 Ohhc 5WmvU;ݰbPixd7P!Fz}gmK'>|n7(]5|U1YU4O8^tf7*_=cȠ=ijëɃœ\C\:^:FNr׫O=TUW颐sPˣo\]EW.xSɌ&)kٸg|6){bF9`tQ(Ю,4Jlr'HQ@/Lf/P&UE\h3 dYժ%Ҏ~)p#dSR'rVBԏ9 )pUVS%d7G^uYq<]Lz@qfk;?;@PyxiT/WFEE&`όpWBRzxǰ _\ҭڇ[P0SPPcD{3vwԗT5e4U{f,RC e oS^*$9lAC^f Cr#H fk t`#s1S+. ~#-5HlEeSُ>Jߐ ZX  bPٝhۦsO[~erguZKog #3;GU|$~E)T_j'Wcmz_; \gcjID& f"nVVh}bE0C4!/d̪lC @SHAb.Ғhzcb(fD@s/ hjS#OoFZdl)T밤P֪>~ZuAh ZюD7`)\rM6 $ 9,ta?@ 8ZΒIA7`QT`g=\^I"{iӆLGLqM<1`f\a@E0HԯTVTRuBo I 4aQ#Ba,B#BH W `|Ia$ sDZ#TMSȒŊTS751)]~˜GVMzؚ^)aF2'sc3JBjSȡB0ҿW2@PK @BrG/&L~ُ$j2+E3YcɟԧTbOk@ealzr.Ћ#41BAh@vHVe F~%hf^dA#J- h"G^ 2J}T,ై`/~'0NFSxPA(˵z²ny䢊`) ? R6i-V]l u(y,TxV_wJ۰f9br>$w"B,Bz;$.Tl@4Wv)2A9AG 1|Մ) BH @;$  =yQy3fj2P`PC~j Mzjy1.iq, I/P-O%_(-lڊ8Iؔw@x6Tc GfF6MAOL:Ɗ3#f^# d _+ DQkUMt'T`F%#-iOӛat*;NHx LH8m5}l ‘,&m2f##`Т/O i;0;3rb#.h'}2U}1嗺7<_Eǎgw?Oغ2Z&\o^#ϊ7lm66f"ܛ:M; /}"@>" BP!lLQ$OB$aAzHO9/zN;Z6>}/nwv;^F /≔%^{U&K_u׽o Ax8B &Vő_I)u4`;KwW~l+Lhj>H~*`{^_p—~>r}1so,:1ųXRP@d˚  奔~:ZNQ_ɸ%{0UYr|G5?K,׮on/立 ~)4s[fYtPa&b zgf7+!T~M<ӬNw4Ơf8[G; Cʲı5bi\֪[BK;vSw Um= bx 1aGӒv=ߑzFab/I0zYx3JMm5k};HMRQ_xXcڵN+GŹ;` b5дEbw24tɓ'O^g*.|fɓi{Dqniиཱུ wߵUյy=׸}wSMGO]Γ]X,x׉f}0@ PハV,}vyT^~'i'+ݟn9az.V,X3Iww ,YʼnDܖO]\un7l&J{ht\ȗ=a\Ǜ !u-Yr@ 3FM@uQe3#'Mqwroy0N|xԕkxeJDDE/|uK6!"""b:4& i-/Mt+sMw'ʊ2bBDDDDDĚ}.݅/t>j]oy d+J@~#1ֶsSmyCssP`ͫos6PZ;'~ݏO}/@O1+-&v8C]|J~+_;^+yK.XyGx^P6;j_99]&ʘYS'N5G߽zO:~rqmNQzwk,^@zZqC:$.U W\TDΘ6iꨡw=dZAhе6;.?S`p9P{wYZKiC: DZ0¹z}ٷZN>2"ǹ6k_9Q# ey#o/äbb" Kۥˎw߾zwWЙsq۩s$KSdzrR_gmcm9mȼe2NS$st0gW4BqKg}XOqnٗzܹWM&CŃU5n.M6)>ܹ^uHIu%z鈅.& 죑pWpe_Qs/m;zر}x={v_,Mݻ'E:`b bc^}tYIn>eU-mpw4=&]fS}#=~禪I]7:.$Md4@ ț~{C4Obί3ƥ8ZAQWOzC,9|{W=gjO<ݧ$Q*8CZNaQu3_>xbOB Z>Ah WykdP:) yEie :TƎJEH& S嚷s}R;O'Ҏ>53v^lʲ:wNnJCV~K9?džtu{89W}dF/Z.K!*U;iD*#"[!Y !vznLX#^|nڍǂ=r_A7U59ԓГTL?v Vzl4g9EL}Ejd2vm=ys#wL?ibTi6gJU|hgPGSS^yUnƩC8;65fV̜M`2KPʲVJR$ ;SGȟlWd|mEAC:4ũ{CqNaw(#?؂pgG9g2ƚ[84;'7s9*4 ^ī*61‰#j3 ?r;nm6f/-O^P_y3%y'7.J^tJR({8BǤܨ"/tN^e6KdUjK\\3TU]f,jiqWToh}iML-S9 us T74Ud\9W(;rPJ엜ATbЍ6if3/$.)l6@&<+N~F/{oOvcCGݮl2Yڄ7o5BPl/b+[{I?DD:\}.؆[ێˎDq Di􃼩,z^cy3K$ soZ2U2ʪ*?lx7ֿ05twV#'Ѫ'ȮzJTN_o W }4W#X&b{ gjݻI5}\sekg-:Hyl49RRCٯ>K}))LyfR {!$H"җ׵Uw  qwS)-=Ԟ4crCgFZ+1@)8aݗ{O2>ڡ&pW.tak3C{9FLdR ~jSO&KuQU,PQk=U !BW@pJWySL|GWL9 \kw_SJKKKKS:v ;PK6lv&Fmi_Uֽs~9ʪʖpJumޑ%hmOKKKKKw}r@! BR$ @%j꛵z3jى78 7]h@T9;Hja_pZ A~`ʾbj@xK>nҌb4–aiFwVKٔrsƫ?x@nZ6roY9ZcݕjcB{QSeEdoW =/vY1!"H&=X!rrio$-2cHt[s[B9698Ef4C#!N=h@b5T#G6Y͖j)d3>c{ uu JЄd +WGF%r܆&Pdhʵ'K]GEye D12,͊j1Q~˯}/y_3,8}iϷПVɔʒStwH(W¨m-ÄswBnZD3Xn|ZNioMm~5gVM*=u9!:ɷ Hul絅Wj#&/SqQ=c`j=azb(gHݡ;vUm?;H^wSSS8YȊ N58]w[3\dQ7E𹥉 sU;oM99tIrv9X䩓/wLJx䅥*Xל?e'>|ј(woє7"(ή͛WH}h_% T5ƨhoXS<4ڴ_LbcOol3ϥ7v8L{}+1΂zw8G0싖_Wz>]U?7r:5&7;د|e/o[y登z [r"'9H` (QcF#PF1ŔEBhIl)ka%Caԛ2#q1`:1'p./_,^}#V*6-S$0X6ԝBP+ H$C #11la @gy}zFB b@(#?՞frz;"Zqy/ߥքi0*y QUÈjv"%nF92gD5BBHm(5SY(0b/ ʔH'#7X+ƶ#[,(nl66*E\K3DMm=eAtfyϥ=Bg=b\"Y5W?բOE7AҴ)*42PSRh8tM92vP\||tÿ7uYi11x+Frвj070 =Y<):6>>&wztD_F(BzaUjs][^1U\0UGG-Dn ]- 0~_2 UIn#c-f 9\jZ3`Xօ.4Ѡ$%{ SHaI  KRSO'i"$.3^9TVVVVVwaڇ8=ݽR~ٹ<ԡx^[WZP07VVնut6TiL{DK֦ƀl>SZM?=ˮh4抬.cg[CyiuUYnzzzz~YR^oַחVeי'ο_\>ܷ,/73===Fkl(HOOʫm%S4'###==8+3#==Q,ޱ^gt5֬7$2D"jҤ\w_jkbrLZ5GEwb1?+!o敕]a_9Ҳ7>($fZ[L:j#4UNo׿m熭ʌe/o++6:+4PZw>#](zUݛeXUe%Q!ۡxbESbf>)BtB#^3fWYu=(6rغ[}q@go8Qus3nW~2old ySKUO${患߿ᄎ O?ͧ W j)6j ;}_?}<ѡWo~6R͍a/(^[*ۋO;2t:s[Q봍>,8O[Ǎ=_d4^WݮG;J+ӻ&vd뵦old:\jЅ-<9$2]Uio֦mu'w#ɵ[5I0 YQ]XujN"/uc1!wPCj9Nw5vvinfDX tftAg2d:]csGc7Awt92Zgы 7!)#~lR~-- m պ]MW qWPf}w}Dwv6TP߮&9zTKv7hW<ΉN2˫F-zQڴ) $Fm4ŠF "lFH!6W lh{B62pm@!X[#f1xdU_;`߼':?_kF8mG/kP*dle2W#s KBYYaڠ%C=MiZj8>݃KiŋAaҬ!Q^<;+;Z&N]5e.aUR*j[>@ȝ<5WW,t 9hkk5|}4%Ar%@7u_;]=ဖ#yEEΚ3g#q3~ɓ?.Y s7a/07UG͎qE4ت:\~t\ABfddMFruvN{)WѰ# ]ō4Mۮ0AF*/?B%8ŘyfM:?j83w5U.5:He27@tZP\]Y .͗:<&c_S.x+3 DbuOp0w~}JzӐ%O7- 8 r:$f^~d%zDP/ #Dwd!,@uȟ@_ O(DUNj-:SѢCђv 3 }$dsR)l?fXQ#P Z\Z@JQO:B zmé?>Q|"3{ ]8|Ӌj +4"yԢ[=J]LMZNiv1Z چC'k{f'/j:@Ukl_۞,gygN=qŴYgr=2!.<U_[XS?± $h)+RH%TP6PKs?|3<o7cJhu5LoBxE(7dRco>plxj[˿RXWioRG'9 Bdb$>=Of}aN*w=|tO?CS}}F*niv Y.!2bb;־tQ4sŘP33w}0[9ĊPHS4 u>n߾~/qĸֳ{R-sqe:N_2&ա̗ Cuǖ'ҜDS[~J_u2\Ph|+:hAV.qt͊BH(0)W֘;Hу~[wܹsK\H>ڼ#?}:РEOf]w׬ݸs΃g :4 q>J覒e؆L5G{wvӥNDs@B/$T;/҇zhCV vנq3gNʁ;,Ih41js6jx7s>=]’\RT3hxҁ"<.>~r\h|#2!G(U =6K))=|C}P'F͘;PںQ+>r ZO|Y+ݑ#AҲoNtǗe gv@Cg[b=bqw/b.}!;b}3 a۪UL-g^ ^$%cnD~6S,ۏP`DJX.EnqZbH",Ua躘@A\!)d/Vv>cB!BY'xq.O]ph_Ye;T^kW1~ԀSe@P/} ~*з7UqnjhoQt4UVUd0j vҾsโI޾R7\qt6cD-Rbن`Q1M .xI0"4VE`/*gW= (PWLm*~d_*GXc4,8F6llc: J},sIݸ[rc a/@Dх[c":ugNo2u¶?2 A7Mjk_CvǓ+j?^}XbrW,>2t^<{ǁ-":YĐx/BNv!Ya1%m!g8r3Rdn\f+JVؿ9hs\ nrT6 Dz(&z4w8k)Kh(apѯOmRlV/-b{uUA*O1+ƃy{{ǻYf-oΔ7u(o—~>Ɵ|HceiU_??WDoG}#LFCYEyڣz@I`6VCV Թ.D"z;Act_Ʃ@L}j$P|2W4|ȡ4/Vwz_,]ilY[IȘu2s r~+~vgDʇܳ)r!QD)[Z*W qTuE~񳩩[F'D)FCUizb|d_(l Fu|HB =kUGMit&žxJQ|$~qMD v 8PPza@ ze%4X]=Nb)i IPV'b*f*&IH3J0x8']|FODX`J;-e) ,u|@HB[`Ryh0r__U<.88D3ӆ-43bؘv@e7['j͢F 9 }m1FbFlQ71nrBxDZ ^5k0 6P1d#+\{@СXZ5;zg"IVS#Y+ s@g2ɥF ua⊏n?QVVVع4q_'2Gl\tY[y{G'Ǎ@`;^QQQQ~fk>Z!eK|@QA&هE*+**s/=G@qXjr?Y8)l"Ӓ/) K,CTlَd DL H \9{vZ6[m"`uː5ըz2a$2*k@([tO~ XF G5 PdlYMOP{,iG6~Q8=5 1 lP}yڂyoM+2m\C\ב4: rߢsi_[~vhwaQe!/I0XܒՇ#nv69 1ئʄj4Z`dwQIY Hd_ZXH@Cb."L9YlG עYݤSlAEJPa)fY$*Th#שM=B*e!36bS0-Ϝ+:@W6^\88XE O|3$ZQ[yy˺uG%6kwpʺOe;~3KR ~ɁA%ٕ8cK鹽{v Zj܆߳ڲ|'5~CEv9z?+i+Tfbo{& Nyvwถ¿*uEپM$@!ztQQ!t*IBzO%f珅e2m'?skιO;"0e+U^r E3U S>#JuErgTuE]`Y91Hѵw]hv0<||pW(UMP?[hU=#TzበƮ_7|x|>5>|?̓[6=h4?7ΆqnCK%n te7`CQNdB̔-gDN5]>N!4Mm7%Zd̒{:?in۝2ی]xnhȾqvLξ[!sN]QvNN `#6jJ]Rb?W6:K/i>gm<)z_[tKMi ډ95z+'jigg>4H vL.Ꜣ:_#bk&牼1`є?sNA1Yxg>0Z𴷆$ueZ(s ǧp_"ǶXE[?m^2MjecTsN~tS[O~}rkMڃM%'4n.~s~-zALiu㷍O=N>;X (IҩF>V6z 02{;_}ߋ%5$d}tPt]Cόa7JI`S >mlu_o+z[nz4KMпo6ڸibhgԆ'*='#=ore o]g~w bG>f\A {JVھ$x_?]b:oEs,>Y1Wuٲ_fڒz[SeSp\;V}{kt@_yb)UVQ{Էls>1ʳD&I%k|wZ1T[BԈo^a41i٣eX%'K-XvyN/Э_Һk>ŪGy=K"Kqy{܃$k/zgl@_0`tDVS"K*ćx\w-ZB}"?mdm;v_) bwfړkV\䡡1Rn߮3\jPg(VnF&8Q~k^er.5v]HU\^}m "ܞ! |#0vAI~ O&?8?/i.;}٧#@:MA3SlKB[ξrLfR϶2rvy? @=?@ +߹~%"ufC1thk =.WUgԌnђRłܫ׵C{$xEe7Z)qp]s J2a|Xo}f%/M3 ey6rm2fT|H7H6~ , zM EYpmwat{wX@tɃJj=>v2rtʂ)CI_x|_a"]f7]Zq-L=' Okc΄۹ YczpLlK:,Tj;|zZvl76g޽5:7"N2Rn)P 4睾G>|T4|SlfWX܋ɑ%;pKY*)i۷ku \QDd|Ѕ'ESD{Sҡ+REGO\ZEpP5;_~g[yAfXB-"#ƥxx"o`xypF_0½Gߔ(WgMYw&Π#XVmFlIm'EB|.@б[|hӒw(fϑfIC^"yJj4GWui~AQZڬrܾqRLu .-&K{]fg3Y`V]&3?xޓ /_\3[y^ӇvϏT=mސbJUm QͿoHrΎV7-ӥ)M={xC>]??-f}փ/MWn?Ǎ #f{g '0-{*us\9YrLO[CEn#ge$yPK 4^J%e?9Nޛ uON$sst+"9^2}E$ =}`wJ]({p-K$壔p p|;% m=&8,.h_/O(5 ?-OL]"B|E~<ݿ< 3Grd8ZnUDxN;-Kig #VԴ 05ԫ-%K$^1|ЍkT]/=$fQ( S0Fn1|n: ٙ|O&9R!gSӓ)ljYDvIχ)xZ]]Wȕg^S >AV#n[q!MF7vN#!d$[{xAKr糢z@ըWۅ0<¤Iͽq}R}x'z蕥-;wqf}Faׇˋ! 2oF oe}WoX$nn|>^uECB.PvZ^\^ 7zuo7bR VUZܐ__Ya|uR*qw>r*謁G)'ɼ<Ѵ!ۄz񁦺X$ /R%jj(z9wt8!t9(݄ۃ}_f_AOߦG5,aq7&,Xʰr !^j}NCx*D8@6h81A8t%4[Kvџ_SfB/U>º˞?bdRʲڨ.B.`d\/qr>E;{uRo@ӻqҖ`B-|޶e_W<*m]= y &uY?G(rά{SKI]|.&>45us_seG:JStze'j@E =Z1uzEhC&$+a]n"ȧ@rY"JZM+ۖ>[!> >|3Ӓ"~AV^Xe)`DZ~,!t l&ht_Xp^Q MzN۔xD_ ٠bMU,p$aFhk*4f8B7Ǎ Vg qs_avFQc!&]Zk|EՓR&8b34*ObiMEYԏ>2z뿾!Y@/I[՛m 4UrGhҠY^BsmE&XFI; 1N6At|M-i0/TCu:ix'BE,Un2U:h}'"[ؔMk B^\W.1Z/o"eAƌ3/f)J >nPPρ"Q_Q G uل( 1֥f_K^^tQK(:xn.nH_ˤ 0y6a65>י\*Kk+nSǷwϬGc7Yb aFՂpI<14%, T*ZJfb3 BVшD".P(zfs(+:5F defD"1jB( qW*f\.7LnnnfYRAE"Nx<OV)+y<@ p*r Ye CY>hZCYgVD"1 eu ¡3 :@T:=밌# t</UrđC?kyNgq\W[[P,j5xr^/JZN:;uzea, ꜑cg:Ν;oBY CQtƍO?E{A{ꅢ(EI&A;5k K,AQի!C8h4(5 BxyE/_!\r%/0##álFQtLE+ ֭[C e =BQ?nݺN:@P={6p޽t&$$`VQQɓbFV`0ֲTVRL&N6 ERaVݵkpΜ9(@SRRݴia ,@Qž={&$$@bvQEŋQvp(6448=z4ܹs(XB?(zرcQUe!q-(>|ðS(ZVV!LJJѣÇ(~'-[({n {=E?aXN:v!EQw߅ٳE͛7Cϟ 鉉rELaؑ#GP]v-a_5ׯ_0lРAekjjP3f ٳ(~}(={B8fFEAA__aڵkQ=rЩ,aIII(:|͛s2 fjdFqP`&v]~}!:2Ά 6OrnS]0H3}D|EerRi61f攻Bz^MKHz0KX 4~HXyy+$qS*NV0qcD&@<ȼ(Yݺ q]󈽂=!f=}A&QbcWLG<хv;FFrA]|ɚQ;7:*nZr߹l_poǞx~zӪ~x~Ͽ>=+}=Hlg '~PtЩ >1ċ5;NCNp'Tu P2դ }'w/>x|8).>uiy>1čX}_X=CI)2c#.]7t%ȱNؤ9'~z'.>.1E_v+,y]۳mӮEָ!>f}ĉOʬhK?!/k농;(FPڵۏުPiV{uPQ¦k,ܤBWT$g_rQ5i6'HviaS1kVUkd2X_y<}8wL&`O:&Yƥ5ʮyy "gC;d2YHL[:)Ø6f, -OP)ʽVStce!CZUv_ͥ=2sxtfϿ*PXC6zz i+YRYxFD?:Dq~V>y=Gn x}`( ̵c: +]e'$,.a ac%()^{o*+߂CG!MNblOv+hݾ_egaR/זs^Q^6T!oBhBÍݚdכmg ȹqgǁ)A>m:9 %:/};'#+lS}O@y֍;L(u y|^U߰H7;{~H9|)aPN/%Ħ1$Z&Zfk@+*5cv*Ύ p`]:s`AWvxkt@OZL71XXTn^򒧮Vnq7dT|TkŤ4/{MMqzs 96uAry i .:}*o!>ufU*Fr/yR&oxq aC<ڿ[ |;:np`ӽ-{qwlmYсe ]-_,7qꐖ>w [;u.M\i7򹱹q !z3".0Oa3\^%zIDJߨ>ށAÎ=-#e{f_s7ˌ0ѣB%  !N8U>~v9 9gΖ"#ҩzwRi{-zEzy]H/C!Ds0F +̒$q#zE%%}3)61e5c2ƥX9 -۬>Qx;{ڸqsޝֵuInd#ڭ5%Gg3r5iE $W=<2 :|VZ ǗO]˫ʧn*=E(*v)ݬH]"d6G ;M_avգcks kis>[joojNghk۵h=Ř{_wlzL7^y鞲Awo?9\yШڿ5dS'j+|L~?̧ߌ _TSm枴>~z.S>)uP`Bw|=gl!}ۄSٸ_'3g*%QA<B_L @i=n2ⱋQc:IjA8a8:. _o/?xpҨۅJBUe@2*|ZKlvv=j=zt.z/t3 L'oEe}t2 iKfw /T aAe5QB>ӭ=)}6( Oxf:km[y !DG'TSbn1eF 3ui7^N`I0]S#.!lCTu͜y.-kX<0x/ &uѣٷ8]dMIeB IM~P9Ƒ\:M[ɄQmyc+?Xv/*̟5eLY \t~}ilPdj.y,L$cԀނh4Rz1zx{#vIJ׆QSl]yι5)/[yS' +ӝ"Oӧ 3BGB_Z{aƻWkA|.J&l>$La؀He7\3`HO OMiJ_Aƒd0  Ʌ`Z|D(q/0D` k?-8dqS>12V@&2.@ˋEuwʲw KOU1ψ_!@[ s*u2_fʝFvY,so:K Oa^",Ԧ'\>(4W=_|"ohGk;Hjk^vХMkf*+MK k?\տ4VZc&NL<*)^YB"ao__ym5̝G NYLǿ~9,ŗ*n͋|j"ZU2?3gfDSv_U7oxmStt^\6 w0 >)V<ͩ  AC a˖-_<<|Uş6<4{Hx#(a#eXR6\76 F~`m!'\-O0,BՕk'1Lm&::{:>Q}Dv |LN_w ;{ܩ-'XzuMYbX)E[/|hRo7N^VݦSBkv[];$K==x\xo_րNs6Y]&efEpsnKXֺ2kB{.2O. F(S1M#e7}ԌJyZ33jA'bcտ{d\"<@FWH oJ[ {Kv c&[KYgW "6Fr\$0>>=)S xx&KS! esdk{%": \qN4 9 q$W.#V(dȔ!ߟWp"Qnb&:2"³CH33{EٌЉeو0y1Vo,19e.D`v-eu׿m 2^G3ˆ bwKX=[x?\:^0OˋT2wh4VRrp+}ӎla2꧷TN[j ٥}L@\qR!@RTTM zZn1{eED.?pqM$n*]"s ( `o7n PV8naD{Ta4 wn9d٣7$#5f3LF1d2Ylvd6[lvf1f9S/pmޓ׊FcOM~AȰh۳rT)x+Nxt?B iBV$ǟ+ fM&f&Ŋ&t5<.)5o_w[?}|h4y ~Ϧs-5000g^_LxwݘwB/WVݝs?rÅ<=ݿ:TZc0S/ZOr/BTIKOf).4e²=bGYAuYTWN20000jFg=KDDO۝WTێf8OF̍Ht: $1۱ϒS̊1rQNbYU{ΒmF~^-Tg 4000SyZ İ?Fvj}MYGM Hugņ0Qz|ێfN\Us{5?/H$l#C|EkVc]ͣ'DMşL0º_;4]U'+nk}^s靅(6>"!+y{1c~ FvboZ ;t|n.Ճ)@DŒa)Ҵ0r3KpyIlu\9{nհ& NaV5 ^/yzt)el8.9$\>" ҡ̐'ltAI'CҎ2Z|i):5FwWsZ0$2HOYɃO-X4,k" ڲJP#DHԽe5_ܽjQ_]ݚ@M9Wn 6H}pɀ":?-$;z}CwsaY~nKYYOXp4K# ڷ0LG8!rU8] %GzSG[eʲ@46a-rTreaf rܛ64DY,Ih)Je#|P}ގ04w}MօlFN~Hz\L 7RR%X"̍Z8vѾ_r) IleX5l$SZM\سc|u N/ˈ7eow;N :z| K 6~uh0ħFkYrN82WҷY Ϯ])g52Z)Tw⢢͌'E DwG[Ev9"-J,6eDAt?ANY _ pA 3CKL3 6bLpA0 #b֌}Ȍu$7ib ͵Ԗd?~\aԻ] _)L%zIx\ө+fbW>7Y8bψv=R9n<,R7n5yy3ו|ub%gnǥs$dmױ'D<Y )Zo\7C(Q3 'JiA AS?~i+:A{@@P՚_fҔ9WE֚ݿbK G& `FDqa xb$yub{g"˴}@Y]_ǠC^șH kM4;>y:8AH z]tIMdgfE+wc k ? KGُ3+.Y *mjCd6l;uɏ#L 7|5Uk2B샤g A`=R'~*+*2. '6}@e6!neZazvo>ՖWjHlO97Mq6[|{7K'NȥGGl54;?xD+_N{ bß}z)峅ѵW;F \m(w exg)*K}|p?]?<=o5Uh'-\>( `{ϖÆuqe痹'F۬ 珈 zmk(ln. A| <47^" Aɩ{Ϲ3וW*=‚dnٶ釖 pСE$^Q*6R, xxsx{ O[fpzKI[@A|Vħ7//9{1yȥgׂ9|/'q9b}}FY|{YZʙk[_I#ua] yav )ƥ|;222r;Y^vS~Z@_O!~iA Q7^yJ}I9t!/M yޑd106$8҉Af#"x3v+ry;lΓƂg4<}\}tӿOSRvAQR[is.T iܨ.kNkcM@l_YYeSC5tD?0` ,{pIp~O16kQ"` zm|}m CrjqbVRo ^W66LS[Ἳg)'r֗Z{ ϛ yOB!St/6^{3K;:/Pl46!M|Ih5(\ ˇf AĠr\3u8) e4N_72M.(wA鉵ן=ȀD0(1PZ P\qǫKՠ:7!5Eϋ ^QR\6^r >A+!nc來%C'BBČ,i9"4K"MF,6' \Jsٖ)s ZZS`p O%tKy| Xv;Xj/"=U"l{U?gfKڦTB5EU+^EQT*ظW,( (EtBI(!$6ew3?6,'3sN6<3w>msv:PY蜡В׃}'Ǒ3^T<16bքj<]W_?2Ii6@162L̓':{\zw~=[XXXX>Bt+Gpua mSHJ@MM!) NS!}Rv]9z o޼yb˓U^_YJٹCSp,;f~V?3_.|5\|r7G^D-OĒZr3ftOeU6BEf 6:Sb/!Mo-.^8B h}c˖-{᧛k?yq9XB^Nt{Fn)Q8`:XgKG3ZĻ~/!u+=UW[ {MgoBpo\ITųyê7p̹{֞ybx態FE}ST ;*|͘ sX" V/`H]F9S #y;u^Z};LMU7˯CR6n3hgU3m#7+2ő'LKݸ~ᘿ!Jo°!rcA~i@rL8zGm0J֚HP{Y]I'ItLGdȂbSˊ\CR1̣iODPqW' ^K߁ReϚ_a6W0/J }E֪+^2FܱwGlԀi.A/k)=s[GOu$ZӒy)Zg~ }dFbhzʑS"25~e'uvT,iK+Lwjis4"”{WkG6LvZVIw7wz3x߼=[xރƯH v|Ʋ qďc@$ IL멼Rz+$UG*sN>S$U<4rGIFI6}1iAze(~U]6V[_[]{#''''' /aQJIhl9\>ʨcGw_N_aӉe9gϝi \]3jmUz}T*8PַQJ)p5V^;/#_/:|$'''¢}e7ACGɅڼڠ JPflײ[qzJYQZHb OGaHS)Y>s˶]^jQ٫soXOLO\عLN3hCK'J>q)x,ҍ> 'SqOMOXܺ՜怑ϭy,ß<+UIs2?2s`=+/҇֡12^FB<ҳ/#kF-7;q&I.=z{ x# V /19wֻ}a{õ^SN30W8tǻ坞UG^QؑOy\K_IE?Z6 y_Gia+e-q:+e2?!8S7DfOCH1xb3 B$z'C,68,[뛫z凫ڗkw y $NIgs-]7K{nO~' ]AL E"@@QCE EbQ*x ݄=3HrRޙw[;nΤC޻ȮwySؘqX8O|lrzdTTfο.@'QQQQQ-:)]Sm q1 ұ\Kɉ>wwZAtTTTTٽf;7 8rjU]p~Aנ鮙AK]ޏWgQswȵ7v@ho6X e70>f](U˅EAQjp VU|&NvVTիN]L@TZ[2qG@_DBB]6ckcSoF+vnU78H\&me3uB9l|jb!;݉ȽR$U8C`?ʙWH ׆s~6~G۫u$ DR'k0KKƙA!(ݶy1?KغUw<=ZC^w>.Ϭ1xmMEq[GCĽE,Ahmʧ~mݸ }+hw58;lڻqJxc6&ȱ FMOy5 MUgw~ خ ~zk xg a5単:7wkΐ=Ol9SOK%֝Xi^1>vWE/j|mH,:>W˚N}ޔBLK;rDڹ#@#FO\ȯ~6b@&QK]gOZΞ0QPY[r@Qs~5^ƔgR)߯\S4me+@o;-r('p[OɮU OdظWor:/Td~᯺بʋjUybv^M.S_o.-;7e ;_A[3vQCqyemMf;}n M36SٲP蝉 ~rѼ݇vr^' ѽa~u4?Me_tW<ĕMVI<0彗96֯HVKk1sLP>`xrV'Јؚ,\);Xf(Taa /)RXm'6JyB,6JCK`VN^Q#b+- ,DO`YTv^ݹtڳXuG=zb^YTv뙬3,43trcvG~yyLxzQu۷?IYɉc_U9hZc?d[#!-7O-)W`/:f߽ c޸ڴ$u=cEά=Hot*jM M1|6xr $Q~G2Kѹ<!tM dG69l+^Bŷ\ B\X./*@#0]̬ή~0i=W_?kˑ?f(5I"7;xzΝ=C_r;[zy L`Zskuta޶7>x3Wo-{^!e..5.#F(D+4Hb, g8]N^+Â(H, ;A6b so' 'g4ɡ1|ŀqRD Sš:m>(_7xOLҨ@MdMEwyzyOڲabo4c6>}]8uw& $7]y t\ر*=v;M&`Ƞxrvju)#H>ĞB7t^%ut5] OW7ߠ9"$ j6f}F̙C_ 6|wn{㒷sLb0C&$3yzrp@K jҦcw|ek0Cn׳7i+8rK0:SsHBG Œޏ$ _<ٖ"F3zTyéѣiBgGXw*#Bˏ+ꄡ,X8VvZ~sl ٚ8W0S~g3H!遗xʓ{xfWp\囫@ aH̓H5?'bڼj??@HEa(sOOϴM') @E"B\xb[IT]]y!ȴ j10lUM>'K(o+7ł–Bڞ8n_ ZjJXUcRz|ErB[3 l׷@Kc+д` 0W^L] } oӟ.*/Š+̙7'V儤=(az/Jc$b-H䰗;" <2@)Sa2:RTrg9ނp[EMO$,hzT "Et^;76QЏdHqgr:5L.]QE!q~'^b.i D#v9ljw(pYQ@nSp2逕'wu _`7/]nN(/| ċÆ$Jn }c׬AAvm{{==>PNă@GS^&bb_P(J=zʫ#0T1 Ox=@GegE]W[V QgsGIU{Us}ַ?n~j20\n+hnrw<6 o}Qb)DϢU.3s( º̯;]Y|Xa{=hʢax,:wjRBdG%\s|qjѮ?^j?njuAVHVgYձrؘ8d '3v]c^7]ckƢiA7q4#<71>Gt1>])l"6AI.SR4L>'(&Z%J KK W1IRH }#*`L*ScG{hΰXTh|&*Mxt||m O es T׬#BqC'H O)t5L:w."'tM"|R P@ Wi{!ʠ`T"OIJТ.\Np;AkKTɣ3yhvt&VT~յR;"¨#:ϕcqyj/RTG߿xN5ws`<;zpnJ.AH> ~KϮe3^nj< v~p8:޿Z7_ϑ*.W4-\3FΏζ+ïis]馸.N}mDқ B^ȉ q.)iBd)n!Ǘ- EJ5E&ũ 46ib%{ö`NE䑃]\ o>p͙cUj??*ɃҪB EQP{׾.䣃#anWQ~^Mue>JнE~*]ƺAS?X}3GJG8$rI 6Wqm9 !Hpw#]wڭ֪7`IMd1zKяL܈uOy Wu;[R[Uy@@$zKqT5pA" Sģ`_j!^a !:B"NdΩ'Pjn>>Ց[-r#737T?rPH$SYxRStPVt)5upi{=ɽwXp[kD;0^wy@l%8x]#&/\٠@ W}-V?I t3$R=El8;)9aV'6&/Us ' O4biO՘Kp!b#@g4$C; "J+ PJ!@tw( GhQ 9 {'zG^-EQu9]25@h.r,ʘ=vmCvdPRA#?EQ'^>+fjW .9鉁!0Mz6x.asg'9ѹ` Tll/m) l\Uh魵\ӇNZ?<^s*kQ??nB|d V3bCH7%"% q <-H[Ma`Dl(

K]?YlF>a <2A,TQAo @!@Jp_y:mNʈ#_`썉\Q[.H/!ӖXM9fxQӭ-w}ozن%݂8 nSۧʅ pXzGܛ"S`{wrma_Sػ#P\e#/bEY®o<=D#,xv wA#v";gI`kpiڎ[l?WÃpZmS ) 5g|K&/xX 短 ;\aF\hWeAtre(EI\/:\| ^FѸ!%Kn\'IG?'(\%| '>9 *b)*mQ'$K7 ɰ1BD%^|Zte%E IT\m皢(45Vjm(:.@NHx9WWXԩi.lM7s-.w|`-QN[/i1Ǫt7UUPh"R|8 NW0pɞgoT4J (6<[bTE$O  `Qmw>d#(`5TW&$QAr!io\.jJ ?>)ܞxwg\,2xJ#bl[O5ŏEWڐgK{iFG(axY'Ҁg,W/%kf){9irA0-$.@ .]ko6\7p~"$.z(hWW@M6' 87\a\qA1t|"&pU0vu?M݀# mZsP>0?u6ap5 #cTe֠O4RR{)Ǡ4\/3 iŠq0k X H kCujYQ7ڃ̜/T$6Si/+$a!dg_.7:z:GD(ڊSs `:,H"4qg)iy<`j T&\nۻԷH\dYWD}fiY[SR/J t%iy ^6:Keb \|Cظ$xl^{A=}>u;U[f(9vR`w̋['p-ǮK5eZP7.A{ C|߶?[N'ġמ:[iǃBlמ_lRT}I7{~խq}cuǷ5/@|)x]MF$y@^% =Yh.<5OAv2(I[ڊ]-Uдc7^+--lV}A4边âT20|6Qtu\WkҰ0_}!roj2VuRDm=#~1̅ffJFS鞏_6hdO{s^ᢺǟ{po?!% JO2헹G7l2'Q$ Ez\'{jqJK3r Ѱ( /74`'W '2~i?i/RXk(jnХnY꛾`i:;LRSm"K b,foNn1>a~~&F57Zar+5=x }!BjW) tuYEO@r7;=3x`#l d\@TRD$ tfԇVtb J W}F`x&E; 썷d.Z"TM |o#Di1_??XL?]Ѭqre6j ԇ{Gƅ3qK5\-7~zF=|e؁˵?丂vnr1_9}EdMeQ%c,馴>2#GKߪm4w8.cm)oDxxsP7n/-o5E.LjukT۶}yq-w?͢]]|dr{_jYm/=YX%S+H|:;/7<<٣(LgY2z?_׷Y }52lhkmh-zbſ^ـQ3DS{?ArqKKpxmfcQUk064קHx!?յkPddn4e !D2߀Psjff>bAE)Q32! b$*BLׯ&UŻ׬ˎyQqrxb":)CX :-LgQNo#eу }ϙcJB|^#y&z' yZB04EWVHbB+ˮGҳB88c7gUaب3'*P#$0uB63؟H89[xCvah! !xC,m.CJ TJ(t@$+J?_&hBTV b46<½ڦBzC.--[?\}TvpXAc}0W遬rT6jtи@1}Nkj:__12AF}15T##zdž@_Qܘ:qDD8;4nZ }("RK8V{ZPe׫*uU{!] o-~)bB#DDV9rP( T:j[fQ $&5W bH H8;q"_.C9w%j+KM 7V9;WGMZ[е,pm/s}s@3/ySd$>tSPUiNv]. 7kU>*%EQ5eg ^_W?!*aLgҒ%ʦPLJQjl,$jݘo\}dƍW W7F&vrQ*^J*'jn䶶GKu rAUi^/S6(UciijuH z翎p8pk CǍN 2O,_#;Z-,{D$7}xV?<1Up:Up\6rE`"(2HӃE[jy5wPW~?Y;}kbJ_ 5r)B2zK 6lI+.,ݞ')4VG ~YF}vJA#V}:12T2nlRΖ\/ z3-*cm!c1X l_py5PlM]cLk[m+{%BD>h5/ag1/O{aۿ30I}Ce[Y DŎ7,ȧP A `ѭ<[6&}}U8H%[젦K}nP+>žm3Ae맍28>ĔT_k#cN~v\@ʯ\_u1r!h 5Q#Ey.[|R@suIkPWYe^AiB?yqkq(R yG'*(%W$r?~~iO~x#mj퟈@XJKEDBxX mQw]YRج|졠o];ro0(e/ܓ h2QpH D3 eJQɿF W5961wlػw ; 7o|?U9jœR5?ł@^+cCߐBhYc@( Z {Z ST6%$=-/p$>ڈC -r$I4kPt9MUV2u`hxBḒM i@ttJ" 0VcS֢ V mZU HGcd!6"Tei147ֵo S :2$@.]WY۽a߮!2؁T,& xZUcͭ:CۚuVo@RJ9uʘ _2%z? pZ[Z V8ZkpZ*8 uBlr9RmTQV_ߤ2E$J\eUd!Ro)V6w\UxB\SjBߠ@Nrc Xr[ @RIxZLZ̥'Jj·[mĪJ&-e5B&:1p݉zɣqgxlzne⇖e`\H_1-{޻"F1S$w]4 xe= /Qf4\owNg"͕{[⁀;le ~$&#`"ؓl1[Md1QKFKceJbxrXYs[ʣܳo#dڎHɏ_>>.K9LfZ @,LvX, u:[an7 BXl6E"P(fY.Z[[vT*u8zB(Hfh B-lr[rI$& E"Q[[dJj"V(X.H(th,[mX)l6X6B6 0bmH$2 &I&QJncX0bG1͌X/Hl6[[[۳cbJJ$6V  0nfe2 R3 B&z1h4zx1" cz{d,lϺnzpu:0X.naxκ cH[La6֭YwζdzBlc≠Yxwܞe V<с(u S% x> 2xq '~\#lyDkaVqq4w’z!iyhʂ=iU__O ٻw/M|0:M'N`fڴi4M gΜ0̱ch~7yh޿?p޼y4M766vǍ!<Ÿ/Bϻem,0uuu4M/\c~!\f M'ON:m^w !?i~뭷<8pas4jƎ?as4/3 i&w1B2l0a>S-[4uVa{1 q0Lqq1Mӏ<0ncKs=Gtvv6pԨQnckkkBCG}0SN1 3eM&YfA=6B~z<!tdh0amի!7nt0̒%KhdclNNMӫVbm0ʕ+=0O> O4Mo޼a.]bfȑ c-Z!ܽ{^{ӧO{5:{cuyw=Ι3f1633W^yc] /<|pիWB+7n0 ӿ}B._Bm6>,Mӗ/_fSCkã*n˦lv6 )dS)JhJ*"("  " В@Bz/em?ٙ7 =8ws~w]Bv&g;~,pȑJUVw8k4Ϝ9T*?sN2&L@1bhLLL49+ʃ-**g,Yb4gF JGPg333J/lrv˖-FqҥB !,..ph4:tHT[իWFc\\!OpB_(ӧOC;ikkS*qqq&g?uLΘ1CT޹sGT.]B]|R!hi:cRkJHm.gF `ydPay&%t!$>$Z' Q6z:qSByl%lw͑.%1d{p4]7jCCU8I*ynn mYARk>4KnB"gEsFVȠ@Y&џd0BQ}.]ZozNrÓ[F1dlP=~/TBC?w\k5`xH sK|jRĦ?! s"Blʡ@d(.$RIaL$ =ƒU ¦j'JJRc<`Ʀ o8M]FQ4gǾ>BC:ރ1+GuӜ" ;r>7Ij˃}g2[^\Ie߫?|A/L`@ԻG~>nw%37iXc9CmYTa<qFKZ"TGZ7yj7z1BF{e?0:P5Hu@d6g>q6 /$I_|4|W] fP+ 5gNʸY="knÉec;)5aY0Sw|VQu`q7=5n(rT yOG1ǰʼnL:U+c۝ i9IVNx\um+ 8nԠ? )ŭb[SjBRK)ox>?qb>uW_m:uDCΝ?O-mO9IVs%S>K=nlS]~ NזxVvM}}XvD6p_>>;*zy;ƽ #bB"Čߡ3.!dhɈpd!5|"FLz/`!y@Oȑ a9Fuٻ%nƶo&,c#WԠ[6Q0iV#~,LmȩֵxuW8>vregpRҷ ~jKcOBI 6x5ԏwrCg\ o}ʛrnm ZW'J 4aTOFSybۖRmcN>XjAm]ڙ[?^rS7}#s us0ٴ}D=!V_:}Qos$!ԜNKT{wdlńًy #1u w/ed"GRHHHXl$fB˭L9!B9<ʭ5U߹|ؗxZ#  tM&OCa ҉";^I}v~ÆˬjRV]|kرcgh6kR?wρG =I^LaFc!|P͌CbM|?gr$b Cdi7s´I zU\4r!{hzpF1n˜1~3/HMj$zXiS&1JLN3ns$eյEw邰K&  1禩O6i҄&Q=LaF dŐ<P6m--Ը&RSI2t~h1ƨ^`hғ1ZLKSq̍tOW>b0?o ]݌޽[)@֮է:Y5MDa,NJuE]]ynw -Ǜ)rlv ݇]hs$2Tg@KK /,RUV]f0$'_R#{9IZԍx8_`5/l-yInK}IV mє_)cCZ Q)" #;p)fmMW'B5yn綄Nníנ`>Xa V[3mU>pa6Y \EPo_Y5ǚ)9㇤VoOV|=b`a{NšNlR=0 #Ϥ"5UE9 1m&hYNhh)|iw9 ȤR5ab_\ ]JoW&'ewS@UAFZe8rTe;:q)ͽc 1k0 Z"s_+uuʢ߈+ ^}=mmw*[v2VY9Q"ڋk`ܤ>+*c3RWu8TlZ}p,&ER%9p|mɺz\ԍlMⶍw^mm+'|=Ȼ|TiogyjF^"{y. hwyZ! 9ikU[x'ߞ8惬1ʳe9ښkKz А~/Q" nXɻszI]]Ga}ztu*[TWoM`؞2ߠo~1dr-X7y=`^f.+ny)@~MdgŃujUH,z%B@Anv!"+W{WcEcFjUQZ !~zOSw? ZM~AJ(s8{?"O+0UC M<3=S u lEe;v@*~𯳞_#uU_ر9M=ed-A EV2~ G&$tݽ3~@)%DY;FPZTeE>.`0 Fh,;oزsG/A\}oUo7mp(m^c%qKDuL7!MJ]3S]Vkk-wAsSZ$NnO^hFm:'/~d;!0%V ; NQGEWG w`Зv"B]bȁm$TU ||0r"Ər<⅏?h=G/I^kfQ̞ ># 0EmpPQP[[Vv=ںv^~,mjtƺɺV*fBMgIc "Yx$V jJ$Y|D\Y|xȐχ BMLc0ZZsj|H[|ANvϽC?76[Y/r+WxasWk;t\.GO ɉ{0aʊ&>ؓmgpcP*(V|>c4U e)S7D.3Wh~x/[d'fPR\h;,"X<{ό/ %c|Uqh\FS&KΑ BSUuU.PD|q U[#O 3rTlezA|6rQolmY=+{F()wW'm)ZK$^*9d+Q($vUV{y/{*c_\sl碟a/g&?_4GsΞ߯j&]@D,ߋ\z -[w[ճa ͑`~/NВIGt혡.\2/-b޺5zBm}iΝ8;\BcK-Ku tV=$-C-+{ygrloä>sQ֨;7~]B#XD%ѡ^cT/ϐ'lM_a*oaQKO@ (#l l!QY %I%FdC )I9b9$ryЀy @z ˦QA^U/h%b`~ę:^zH[g|G*x@{Cyi ̳b]kk_tP}W'gS"U> !csvya?-vɿFu iWmήPru_R{,زxl8h0x7FE}_9R#BՀ"5HTGH  [ {"yRy1V,9-tTK9J1M.kT$r}@DTh>iQAG:q@X@Հo7BhPm]&M ɵi !}hDc@RL0L"8x,XIJL~pr˗/?qsל(o|dc['Z 8:3_[Ҧ [.q.uCaV̓ 1zx+/-iCsXS[m]7zr+N5Jr/ sw,׀=H9-dl`O,3&3M<2]9L$FO .iX0ld>s̰ØS*:x.L62ƼK>[ RۓI-مQcC!gA<[/%-<`{35CнHHT00ZȞFuJ[ŬS˖YlFG63lYK$ 0)y|`*V) Baȑb]b#W<3dVCylS 5G#rh IvHɼ'P &lH$I *9}~gvxH䎌0# B0m7]2rUڔAJYrmU3#GŇ{Hw^^|iٴ甝t %Uu"c l{Z-o)<\'K%Ée,@ldUEƚ9OŨ U  iHE`O1&UĚL&쒴Id!Ũi+2ve" Ek,T[a6ԅk) rդo:JlTlQn7HXHقEdK#2OjGz*Ycx jHol}5<#{Yuk+I[ݢ)|\|O_ ŏ(!l(JI2䟸<"W"`2 4&d#kK#Ӄ)%a3&H.Qa.wV]XbcqF;G[7n%llP !qT":,Á.\H|TH@w!K(&=M, !`aPe8foI]Zp!&y \;ܓO 벢ȹY88of k$!@e(gg蹤.% X sd ٟUl`y, KlE4H;sXGxj's]i@}SޞlT޶^=Sy(Vi0Ϥ1a}խXvQc~I}T@M`.,$j}fc>?   r GOlG3 lH:gtYg;~W!μѱ/mSB3Jޒt\%BCIK4Pu +٤ LEܻւ?U$Ч f~y* F&d {D-˴o.PXqn'C^|NA.!~bb)Rpc/L}oa@7?;dȐacߒ@:Ժccccc'ߙMfa F%EYba;3d+ܫKkޙA Nz@& f:0װ5LHa$0o:q{mR/6+7juƦs &vJyҵf:111LFg<9ii?٦5"s7WEaTd-j#Pi20 F\pl4ȮC.܋ᓻmQV-rه{9'[p %Z(`byR]A<ע=ػ8Ys跄'Oݢ-8;8Ma]smNL~yl={Ǿ*F&G%L''!̽_;o>hc7unr˫p-E>mj1|}^uB<~`sCҗ1Ȗ*/O7v6fnxTz /NX%\~ϟnne :Jd|M_ _pњdY J"Fw5ra{#?]Ja:ZL +/U /߾ꝏΉV(K6*lSG]~޺!/-ғF) "|ɵٶnUK& [q@-j`/ bƏ @s^(ƽāg/t淆DN>Z[~ m!hHyB=yٞ;uݪ;~e bsK-D{DM>VTBg`Hа!r[V[I~6нᩆ%+Z[ψ7XwhxM?0lXp]M} P_vE߇}}Ƌ诅>|31 2@TݠB^xdhۤV5+Rݾ|ވU(^+Р:B3~G[K6>>zswvhWoբQ{*n6>G>CՓT,Z:s#C 9{J٣^|֏L^?y)(x#W\m[AlB  uw;6S A[ʘjDq l/]z!4LzuNlXXXX?O'k@!C}$;F1yT޸T9z[Epd߰Сږ6sFnu. GB*>"@aZғqY d㷤"+Z6i۷l/S_YówM݉ff߻~|զߝ9h%#j_~ڽ5kkQo_wC3 ^WM~x`QSٹ]]+OYnX\ au+VvG[NmfyyQNLt$`=i5P1IOAW C dɛCbbLܚ}R14.wݽ|!xҢ*؜z?r  LO9$tC?0 ZнAXއhn9^Gnv&Ξ-&-qr$Af?6Mz G{e_a^\դ'_=D778ɀ  ?radog41%R2+*R.?z>v玾5Wx/Nd'\='LjNuwυ/ zo,Ӓ\B KHyae_@UV{Ce2ro;U`xHr:o,Oi@Pus?|u篖Gm'M[A̽׀7u**iM$L'7ITFaj[ Vi/.x{٤0tsYʢ'GX[wl,3475 .~~/ϞMbUqصkWL6"?*л><KEGxI!ZUͽX\?5α=kދJA=BU%?y)% rśdc'1*C'kCE,͔|"H@T cdrHk*:ܓ+:;{x7'g\wvr1 S\ Uw~%Je)i}u3u:pfh| 㓤Zda&j@gggI_ɮ9f}v_];0ڠi,i5ïTȝ_[N{Np2c:M~aQs3%Φ/ Д4ɇǛdR!4?0gV]j.ږ?hsp@F* 4ueXpƊ@a֖jܰ|DsK,MQ[x`w$x=FOT_Kl5<ɴA6IhyLjRGKSvM=ؽdcO@KiI 44\߱H =V jRE.Ol/w6MS}Uar?qmcA";&k 6(fKTz#]*뗏1>>0.Pը < vA!8<6g-;~b>@C{mVNAhuqjiаiW^v"0?;MףZeɸQBuU5vW7gO>~V?(|x<wh7,7B#0X1I 692O^F*(037Rѫn֖^wζS[ ]B؞@#t,2n<6N(-*tH w1$mQY"RaS5`A`l \Z AN {<'-׿bӴ'rmoӻݽEw/6C_u|?>_]:u$7!lW@9<&o s3k Y^ӯ93**jت{^S_^<,$O;|P4^66V߾䯐?v緵úԻN"]edC,Ibmê7k#VUPRflST5K)c 1hkFW|!r6kCyF`#y] u &G)ŠKX<2mMi|k$ǂ uCs&uswƮ;iY-l5t.JBǎAQ;10O @Ǟr2 0,ԣ!GR. ^V$R:AXZ9HR5b |Ms*KD2)2BPk/O?q EN&nNR mu  Ӕ6E){ȤF~oʠt 7f>HJ^1ݪ8I"lôf1S JCl 9agВVȮIjl#k: n[AP.fgyV+[@7`d]`-ax<^>^ ? {P_g7 Ogny3_ JEdi?Ѧ8#N /Z|w8ۥ3z;EM*kuUg:1d2^>_oIrRs]2_}= .(u0LÔ{3Z2!ЩK4Fw7gA,8y("T"ԄOOSs |[CL Q]_ ASsÅ/']P۬V79{pa͚q C4jZ "Q ;U`a3X1mvlك <*j2uwmTb0m-uFUƭĻb>y9c_-\zTwJ@[♣6=^@kз՗Oy qϋ_56O.IF-.ZemO\Xs|ء)au^ })M 0 t%3o'޲ Yߺtbo% z#!5/}IPdb.,edl⿺;k5M\]e/~Aǚs}yqՕ+? sD 0wpvW\qeZd792Adt<5)%Teņ~]ŭu%iHDxOw^\x7Raq=S#  v p6wVl7Q[}M(x)Cziv^sãƅlCfbƊy%]'-}/ec"W{Xytߕ,~ss@2P|IIII1ÃdrM2FܽO]c^_Mo((DXCy-IؔA>2_vѤf i}VpuũS<{}k-=XT{vX,*XՠłIc0"V-m灤VQ/߫c|ULZJ.U:>V~r?t2zx70Q is2u-~2hQ}%S4G z ̳slD2?!n$]8 jE.I>ż =bD6`n>i$-$9`PVHKĚ\j:QzZG Gs5Ć{-5INohͺqA݈DtINJUk'CM3$wߪA̜%^EIs]f8![E]6r (~Tĝu֮w;ǿ1k֬(t6Q{{ ǿo '^-%_ɹ5XSn%zj-$ U`,yܝgK8diAKlor?[@Ϩɖ]OqMEl-V_r(aA-){y1QUx6Ae㑚}[a-HTI@CdBI1@MbL`> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 518>> stream xA 0 =" ʝ<2;EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$EX$) endstream endobj 364 0 obj << /Type /XObject /Subtype /Image /Width 600 /Height 196 /SMask 363 0 R /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 3 /Columns 600 /BitsPerComponent 8>> /ColorSpace /DeviceRGB /BitsPerComponent 8 /Length 79658>> stream x}gxT:3)LzޛzG,(\Ć]{-XbWP5*Bzg2ICȰ>{L>C}^'yy8== ӏd6 Űb0VÙU0fߩ_c+$BPCOrt0$۶v-A@+}PCܲE0`w?B~VH:b#EYBQ B٨0 O%Ǝ"Ӧf#tVH"VA"$2fW3}>rS#;>]1)/%Eɟ"aq/mm\1ݱCU"H,+0]jZ>KU ()&%#tyLUs $cE1GV$ŋT0P`QOOF!l9ΈPV1ɐ(dFi@71U(2Pը Bjb H*&1D,Oj+F:]9ǜK ~EPquAYQazYK]O!) U wF5$^BAN OR b䉩V *TdD5,@؀=<(^.Ȳ"7EbBHnSjЊBdU,j9n#<B* &AL #eƧtK fQ<cB*wF@9*`~DP";i#DQV<[j `bH!Bs0 Ȟ'Mo>1- )E$O6U"86qiU}⦮)˒ i|B>dֽz_ȟ(s4PNޢmx0V-b*&K&$[KMa$d"IL8f71LFV a:ɸ峒\V4/+S *VPdG62 ; Y#@"ܦbX 3=64-v{BY Y] ۆ[xl0ԏ F=LY'R6<<&Ԏ YGWͱ=jr `?>ݵM io ϖQSgN%Rd#= _G,.Gyz v *~[3*̅' Y!hX݉9y{8D’D楺HPALŬ#!3 Ohhc 5WmvU;ݰbPixd7P!Fz}gmK'>|n7(]5|U1YU4O8^tf7*_=cȠ=ijëɃœ\C\:^:FNr׫O=TUW颐sPˣo\]EW.xSɌ&)kٸg|6){bF9`tQ(Ю,4Jlr'HQ@/Lf/P&UE\h3 dYժ%Ҏ~)p#dSR'rVBԏ9 )pUVS%d7G^uYq<]Lz@qfk;?;@PyxiT/WFEE&`όpWBRzxǰ _\ҭڇ[P0SPPcD{3vwԗT5e4U{f,RC e oS^*$9lAC^f Cr#H fk t`#s1S+. ~#-5HlEeSُ>Jߐ ZX  bPٝhۦsO[~erguZKog #3;GU|$~E)T_j'Wcmz_; \gcjID& f"nVVh}bE0C4!/d̪lC @SHAb.Ғhzcb(fD@s/ hjS#OoFZdl)T밤P֪>~ZuAh ZюD7`)\rM6 $ 9,ta?@ 8ZΒIA7`QT`g=\^I"{iӆLGLqM<1`f\a@E0HԯTVTRuBo I 4aQ#Ba,B#BH W `|Ia$ sDZ#TMSȒŊTS751)]~˜GVMzؚ^)aF2'sc3JBjSȡB0ҿW2@PK @BrG/&L~ُ$j2+E3YcɟԧTbOk@ealzr.Ћ#41BAh@vHVe F~%hf^dA#J- h"G^ 2J}T,ై`/~'0NFSxPA(˵z²ny䢊`) ? R6i-V]l u(y,TxV_wJ۰f9br>$w"B,Bz;$.Tl@4Wv)2A9AG 1|Մ) BH @;$  =yQy3fj2P`PC~j Mzjy1.iq, I/P-O%_(-lڊ8Iؔw@x6Tc GfF6MAOL:Ɗ3#f^# d _+ DQkUMt'T`F%#-iOӛat*;NHx LH8m5}l ‘,&m2f##`Т/O i;0;3rb#.h'}2U}1嗺7<_Eǎgw?Oغ2Z&\o^#ϊ7lm66f"ܛ:M; /}"@>" BP!lLQ$OB$aAzHO9/zN;Z6>}/nwv;^F /≔%^{U&K_u׽o Ax8B &Vő_I)u4`;KwW~l+Lhj>H~*`{^_p—~>r}1so,:1ųXRP@d˚  奔~:ZNQ_ɸ%{0UYr|G5?K,׮on/立 ~)4s[fYtPa&b zgf7+!T~M<ӬNw4Ơf8[G; Cʲı5bi\֪[BK;vSw Um= bx 1aGӒv=ߑzFab/I0zYx3JMm5k};HMRQ_xXcڵN+GŹ;` b5дEbw24tɓ'O^g*.|fɓi{Dqniиཱུ wߵUյy=׸}wSMGO]Γ]X,x׉f}0@ PハV,}vyT^~'i'+ݟn9az.V,X3Iww ,YʼnDܖO]\un7l&J{ht\ȗ=a\Ǜ !u-Yr@ 3FM@uQe3#'Mqwroy0N|xԕkxeJDDE/|uK6!"""b:4& i-/Mt+sMw'ʊ2bBDDDDDĚ}.݅/t>j]oy d+J@~#1ֶsSmyCssP`ͫos6PZ;'~ݏO}/@O1+-&v8C]|J~+_;^+yK.XyGx^P6;j_99]&ʘYS'N5G߽zO:~rqmNQzwk,^@zZqC:$.U W\TDΘ6iꨡw=dZAhе6;.?S`p9P{wYZKiC: DZ0¹z}ٷZN>2"ǹ6k_9Q# ey#o/äbb" Kۥˎw߾zwWЙsq۩s$KSdzrR_gmcm9mȼe2NS$st0gW4BqKg}XOqnٗzܹWM&CŃU5n.M6)>ܹ^uHIu%z鈅.& 죑pWpe_Qs/m;zر}x={v_,Mݻ'E:`b bc^}tYIn>eU-mpw4=&]fS}#=~禪I]7:.$Md4@ ț~{C4Obί3ƥ8ZAQWOzC,9|{W=gjO<ݧ$Q*8CZNaQu3_>xbOB Z>Ah WykdP:) yEie :TƎJEH& S嚷s}R;O'Ҏ>53v^lʲ:wNnJCV~K9?džtu{89W}dF/Z.K!*U;iD*#"[!Y !vznLX#^|nڍǂ=r_A7U59ԓГTL?v Vzl4g9EL}Ejd2vm=ys#wL?ibTi6gJU|hgPGSS^yUnƩC8;65fV̜M`2KPʲVJR$ ;SGȟlWd|mEAC:4ũ{CqNaw(#?؂pgG9g2ƚ[84;'7s9*4 ^ī*61‰#j3 ?r;nm6f/-O^P_y3%y'7.J^tJR({8BǤܨ"/tN^e6KdUjK\\3TU]f,jiqWToh}iML-S9 us T74Ud\9W(;rPJ엜ATbЍ6if3/$.)l6@&<+N~F/{oOvcCGݮl2Yڄ7o5BPl/b+[{I?DD:\}.؆[ێˎDq Di􃼩,z^cy3K$ soZ2U2ʪ*?lx7ֿ05twV#'Ѫ'ȮzJTN_o W }4W#X&b{ gjݻI5}\sekg-:Hyl49RRCٯ>K}))LyfR {!$H"җ׵Uw  qwS)-=Ԟ4crCgFZ+1@)8aݗ{O2>ڡ&pW.tak3C{9FLdR ~jSO&KuQU,PQk=U !BW@pJWySL|GWL9 \kw_SJKKKKS:v ;PK6lv&Fmi_Uֽs~9ʪʖpJumޑ%hmOKKKKKw}r@! BR$ @%j꛵z3jى78 7]h@T9;Hja_pZ A~`ʾbj@xK>nҌb4–aiFwVKٔrsƫ?x@nZ6roY9ZcݕjcB{QSeEdoW =/vY1!"H&=X!rrio$-2cHt[s[B9698Ef4C#!N=h@b5T#G6Y͖j)d3>c{ uu JЄd +WGF%r܆&Pdhʵ'K]GEye D12,͊j1Q~˯}/y_3,8}iϷПVɔʒStwH(W¨m-ÄswBnZD3Xn|ZNioMm~5gVM*=u9!:ɷ Hul絅Wj#&/SqQ=c`j=azb(gHݡ;vUm?;H^wSSS8YȊ N58]w[3\dQ7E𹥉 sU;oM99tIrv9X䩓/wLJx䅥*Xל?e'>|ј(woє7"(ή͛WH}h_% T5ƨhoXS<4ڴ_LbcOol3ϥ7v8L{}+1΂zw8G0싖_Wz>]U?7r:5&7;د|e/o[y登z [r"'9H` (QcF#PF1ŔEBhIl)ka%Caԛ2#q1`:1'p./_,^}#V*6-S$0X6ԝBP+ H$C #11la @gy}zFB b@(#?՞frz;"Zqy/ߥքi0*y QUÈjv"%nF92gD5BBHm(5SY(0b/ ʔH'#7X+ƶ#[,(nl66*E\K3DMm=eAtfyϥ=Bg=b\"Y5W?բOE7AҴ)*42PSRh8tM92vP\||tÿ7uYi11x+Frвj070 =Y<):6>>&wztD_F(BzaUjs][^1U\0UGG-Dn ]- 0~_2 UIn#c-f 9\jZ3`Xօ.4Ѡ$%{ SHaI  KRSO'i"$.3^9TVVVVVwaڇ8=ݽR~ٹ<ԡx^[WZP07VVնut6TiL{DK֦ƀl>SZM?=ˮh4抬.cg[CyiuUYnzzzz~YR^oַחVeי'ο_\>ܷ,/73===Fkl(HOOʫm%S4'###==8+3#==Q,ޱ^gt5֬7$2D"jҤ\w_jkbrLZ5GEwb1?+!o敕]a_9Ҳ7>($fZ[L:j#4UNo׿m熭ʌe/o++6:+4PZw>#](zUݛeXUe%Q!ۡxbESbf>)BtB#^3fWYu=(6rغ[}q@go8Qus3nW~2old ySKUO${患߿ᄎ O?ͧ W j)6j ;}_?}<ѡWo~6R͍a/(^[*ۋO;2t:s[Q봍>,8O[Ǎ=_d4^WݮG;J+ӻ&vd뵦old:\jЅ-<9$2]Uio֦mu'w#ɵ[5I0 YQ]XujN"/uc1!wPCj9Nw5vvinfDX tftAg2d:]csGc7Awt92Zgы 7!)#~lR~-- m պ]MW qWPf}w}Dwv6TP߮&9zTKv7hW<ΉN2˫F-zQڴ) $Fm4ŠF "lFH!6W lh{B62pm@!X[#f1xdU_;`߼':?_kF8mG/kP*dle2W#s KBYYaڠ%C=MiZj8>݃KiŋAaҬ!Q^<;+;Z&N]5e.aUR*j[>@ȝ<5WW,t 9hkk5|}4%Ar%@7u_;]=ဖ#yEEΚ3g#q3~ɓ?.Y s7a/07UG͎qE4ت:\~t\ABfddMFruvN{)WѰ# ]ō4Mۮ0AF*/?B%8ŘyfM:?j83w5U.5:He27@tZP\]Y .͗:<&c_S.x+3 DbuOp0w~}JzӐ%O7- 8 r:$f^~d%zDP/ #Dwd!,@uȟ@_ O(DUNj-:SѢCђv 3 }$dsR)l?fXQ#P Z\Z@JQO:B zmé?>Q|"3{ ]8|Ӌj +4"yԢ[=J]LMZNiv1Z چC'k{f'/j:@Ukl_۞,gygN=qŴYgr=2!.<U_[XS?± $h)+RH%TP6PKs?|3<o7cJhu5LoBxE(7dRco>plxj[˿RXWioRG'9 Bdb$>=Of}aN*w=|tO?CS}}F*niv Y.!2bb;־tQ4sŘP33w}0[9ĊPHS4 u>n߾~/qĸֳ{R-sqe:N_2&ա̗ Cuǖ'ҜDS[~J_u2\Ph|+:hAV.qt͊BH(0)W֘;Hу~[wܹsK\H>ڼ#?}:РEOf]w׬ݸs΃g :4 q>J覒e؆L5G{wvӥNDs@B/$T;/҇zhCV vנq3gNʁ;,Ih41js6jx7s>=]’\RT3hxҁ"<.>~r\h|#2!G(U =6K))=|C}P'F͘;PںQ+>r ZO|Y+ݑ#AҲoNtǗe gv@Cg[b=bqw/b.}!;b}3 a۪UL-g^ ^$%cnD~6S,ۏP`DJX.EnqZbH",Ua躘@A\!)d/Vv>cB!BY'xq.O]ph_Ye;T^kW1~ԀSe@P/} ~*з7UqnjhoQt4UVUd0j vҾsโI޾R7\qt6cD-Rbن`Q1M .xI0"4VE`/*gW= (PWLm*~d_*GXc4,8F6llc: J},sIݸ[rc a/@Dх[c":ugNo2u¶?2 A7Mjk_CvǓ+j?^}XbrW,>2t^<{ǁ-":YĐx/BNv!Ya1%m!g8r3Rdn\f+JVؿ9hs\ nrT6 Dz(&z4w8k)Kh(apѯOmRlV/-b{uUA*O1+ƃy{{ǻYf-oΔ7u(o—~>Ɵ|HceiU_??WDoG}#LFCYEyڣz@I`6VCV Թ.D"z;Act_Ʃ@L}j$P|2W4|ȡ4/Vwz_,]ilY[IȘu2s r~+~vgDʇܳ)r!QD)[Z*W qTuE~񳩩[F'D)FCUizb|d_(l Fu|HB =kUGMit&žxJQ|$~qMD v 8PPza@ ze%4X]=Nb)i IPV'b*f*&IH3J0x8']|FODX`J;-e) ,u|@HB[`Ryh0r__U<.88D3ӆ-43bؘv@e7['j͢F 9 }m1FbFlQ71nrBxDZ ^5k0 6P1d#+\{@СXZ5;zg"IVS#Y+ s@g2ɥF ua⊏n?QVVVع4q_'2Gl\tY[y{G'Ǎ@`;^QQQQ~fk>Z!eK|@QA&هE*+**s/=G@qXjr?Y8)l"Ӓ/) K,CTlَd DL H \9{vZ6[m"`uː5ըz2a$2*k@([tO~ XF G5 PdlYMOP{,iG6~Q8=5 1 lP}yڂyoM+2m\C\ב4: rߢsi_[~vhwaQe!/I0XܒՇ#nv69 1ئʄj4Z`dwQIY Hd_ZXH@Cb."L9YlG עYݤSlAEJPa)fY$*Th#שM=B*e!36bS0-Ϝ+:@W6^\88XE O|3$ZQ[yy˺uG%6kwpʺOe;~3KR ~ɁA%ٕ8cK鹽{v Zj܆߳ڲ|'5~CEv9z?+i+Tfbo{& Nyvwถ¿*uEپM$@!ztQQ!t*IBzO%f珅e2m'?skιO;"0e+U^r E3U S>#JuErgTuE]`Y91Hѵw]hv0<||pW(UMP?[hU=#TzበƮ_7|x|>5>|?̓[6=h4?7ΆqnCK%n te7`CQNdB̔-gDN5]>N!4Mm7%Zd̒{:?in۝2ی]xnhȾqvLξ[!sN]QvNN `#6jJ]Rb?W6:K/i>gm<)z_[tKMi ډ95z+'jigg>4H vL.Ꜣ:_#bk&牼1`є?sNA1Yxg>0Z𴷆$ueZ(s ǧp_"ǶXE[?m^2MjecTsN~tS[O~}rkMڃM%'4n.~s~-zALiu㷍O=N>;X (IҩF>V6z 02{;_}ߋ%5$d}tPt]Cόa7JI`S >mlu_o+z[nz4KMпo6ڸibhgԆ'*='#=ore o]g~w bG>f\A {JVھ$x_?]b:oEs,>Y1Wuٲ_fڒz[SeSp\;V}{kt@_yb)UVQ{Էls>1ʳD&I%k|wZ1T[BԈo^a41i٣eX%'K-XvyN/Э_Һk>ŪGy=K"Kqy{܃$k/zgl@_0`tDVS"K*ćx\w-ZB}"?mdm;v_) bwfړkV\䡡1Rn߮3\jPg(VnF&8Q~k^er.5v]HU\^}m "ܞ! |#0vAI~ O&?8?/i.;}٧#@:MA3SlKB[ξrLfR϶2rvy? @=?@ +߹~%"ufC1thk =.WUgԌnђRłܫ׵C{$xEe7Z)qp]s J2a|Xo}f%/M3 ey6rm2fT|H7H6~ , zM EYpmwat{wX@tɃJj=>v2rtʂ)CI_x|_a"]f7]Zq-L=' Okc΄۹ YczpLlK:,Tj;|zZvl76g޽5:7"N2Rn)P 4睾G>|T4|SlfWX܋ɑ%;pKY*)i۷ku \QDd|Ѕ'ESD{Sҡ+REGO\ZEpP5;_~g[yAfXB-"#ƥxx"o`xypF_0½Gߔ(WgMYw&Π#XVmFlIm'EB|.@б[|hӒw(fϑfIC^"yJj4GWui~AQZڬrܾqRLu .-&K{]fg3Y`V]&3?xޓ /_\3[y^ӇvϏT=mސbJUm QͿoHrΎV7-ӥ)M={xC>]??-f}փ/MWn?Ǎ #f{g '0-{*us\9YrLO[CEn#ge$yPK 4^J%e?9Nޛ uON$sst+"9^2}E$ =}`wJ]({p-K$壔p p|;% m=&8,.h_/O(5 ?-OL]"B|E~<ݿ< 3Grd8ZnUDxN;-Kig #VԴ 05ԫ-%K$^1|ЍkT]/=$fQ( S0Fn1|n: ٙ|O&9R!gSӓ)ljYDvIχ)xZ]]Wȕg^S >AV#n[q!MF7vN#!d$[{xAKr糢z@ըWۅ0<¤Iͽq}R}x'z蕥-;wqf}Faׇˋ! 2oF oe}WoX$nn|>^uECB.PvZ^\^ 7zuo7bR VUZܐ__Ya|uR*qw>r*謁G)'ɼ<Ѵ!ۄz񁦺X$ /R%jj(z9wt8!t9(݄ۃ}_f_AOߦG5,aq7&,Xʰr !^j}NCx*D8@6h81A8t%4[Kvџ_SfB/U>º˞?bdRʲڨ.B.`d\/qr>E;{uRo@ӻqҖ`B-|޶e_W<*m]= y &uY?G(rά{SKI]|.&>45us_seG:JStze'j@E =Z1uzEhC&$+a]n"ȧ@rY"JZM+ۖ>[!> >|3Ӓ"~AV^Xe)`DZ~,!t l&ht_Xp^Q MzN۔xD_ ٠bMU,p$aFhk*4f8B7Ǎ Vg qs_avFQc!&]Zk|EՓR&8b34*ObiMEYԏ>2z뿾!Y@/I[՛m 4UrGhҠY^BsmE&XFI; 1N6At|M-i0/TCu:ix'BE,Un2U:h}'"[ؔMk B^\W.1Z/o"eAƌ3/f)J >nPPρ"Q_Q G uل( 1֥f_K^^tQK(:xn.nH_ˤ 0y6a65>י\*Kk+nSǷwϬGc7Yb aFՂpI<14%, T*ZJfb3 BVшD".P(zfs(+:5F defD"1jB( qW*f\.7LnnnfYRAE"Nx<OV)+y<@ p*r Ye CY>hZCYgVD"1 eu ¡3 :@T:=밌# t</UrđC?kyNgq\W[[P,j5xr^/JZN:;uzea, ꜑cg:Ν;oBY CQtƍO?E{A{ꅢ(EI&A;5k K,AQի!C8h4(5 BxyE/_!\r%/0##álFQtLE+ ֭[C e =BQ?nݺN:@P={6p޽t&$$`VQQɓbFV`0ֲTVRL&N6 ERaVݵkpΜ9(@SRRݴia ,@Qž={&$$@bvQEŋQvp(6448=z4ܹs(XB?(zرcQUe!q-(>|ðS(ZVV!LJJѣÇ(~'-[({n {=E?aXN:v!EQw߅ٳE͛7Cϟ 鉉rELaؑ#GP]v-a_5ׯ_0lРAekjjP3f ٳ(~}(={B8fFEAA__aڵkQ=rЩ,aIII(:|͛s2 fjdFqP`&v]~}!:2Ά 6OrnS]0H3}D|EerRi61f攻Bz^MKHz0KX 4~HXyy+$qS*NV0qcD&@<ȼ(Yݺ q]󈽂=!f=}A&QbcWLG<хv;FFrA]|ɚQ;7:*nZr߹l_poǞx~zӪ~x~Ͽ>=+}=Hlg '~PtЩ >1ċ5;NCNp'Tu P2դ }'w/>x|8).>uiy>1čX}_X=CI)2c#.]7t%ȱNؤ9'~z'.>.1E_v+,y]۳mӮEָ!>f}ĉOʬhK?!/k농;(FPڵۏުPiV{uPQ¦k,ܤBWT$g_rQ5i6'HviaS1kVUkd2X_y<}8wL&`O:&Yƥ5ʮyy "gC;d2YHL[:)Ø6f, -OP)ʽVStce!CZUv_ͥ=2sxtfϿ*PXC6zz i+YRYxFD?:Dq~V>y=Gn x}`( ̵c: +]e'$,.a ac%()^{o*+߂CG!MNblOv+hݾ_egaR/זs^Q^6T!oBhBÍݚdכmg ȹqgǁ)A>m:9 %:/};'#+lS}O@y֍;L(u y|^U߰H7;{~H9|)aPN/%Ħ1$Z&Zfk@+*5cv*Ύ p`]:s`AWvxkt@OZL71XXTn^򒧮Vnq7dT|TkŤ4/{MMqzs 96uAry i .:}*o!>ufU*Fr/yR&oxq aC<ڿ[ |;:np`ӽ-{qwlmYсe ]-_,7qꐖ>w [;u.M\i7򹱹q !z3".0Oa3\^%zIDJߨ>ށAÎ=-#e{f_s7ˌ0ѣB%  !N8U>~v9 9gΖ"#ҩzwRi{-zEzy]H/C!Ds0F +̒$q#zE%%}3)61e5c2ƥX9 -۬>Qx;{ڸqsޝֵuInd#ڭ5%Gg3r5iE $W=<2 :|VZ ǗO]˫ʧn*=E(*v)ݬH]"d6G ;M_avգcks kis>[joojNghk۵h=Ř{_wlzL7^y鞲Awo?9\yШڿ5dS'j+|L~?̧ߌ _TSm枴>~z.S>)uP`Bw|=gl!}ۄSٸ_'3g*%QA<B_L @i=n2ⱋQc:IjA8a8:. _o/?xpҨۅJBUe@2*|ZKlvv=j=zt.z/t3 L'oEe}t2 iKfw /T aAe5QB>ӭ=)}6( Oxf:km[y !DG'TSbn1eF 3ui7^N`I0]S#.!lCTu͜y.-kX<0x/ &uѣٷ8]dMIeB IM~P9Ƒ\:M[ɄQmyc+?Xv/*̟5eLY \t~}ilPdj.y,L$cԀނh4Rz1zx{#vIJ׆QSl]yι5)/[yS' +ӝ"Oӧ 3BGB_Z{aƻWkA|.J&l>$La؀He7\3`HO OMiJ_Aƒd0  Ʌ`Z|D(q/0D` k?-8dqS>12V@&2.@ˋEuwʲw KOU1ψ_!@[ s*u2_fʝFvY,so:K Oa^",Ԧ'\>(4W=_|"ohGk;Hjk^vХMkf*+MK k?\տ4VZc&NL<*)^YB"ao__ym5̝G NYLǿ~9,ŗ*n͋|j"ZU2?3gfDSv_U7oxmStt^\6 w0 >)V<ͩ  AC a˖-_<<|Uş6<4{Hx#(a#eXR6\76 F~`m!'\-O0,BՕk'1Lm&::{:>Q}Dv |LN_w ;{ܩ-'XzuMYbX)E[/|hRo7N^VݦSBkv[];$K==x\xo_րNs6Y]&efEpsnKXֺ2kB{.2O. F(S1M#e7}ԌJyZ33jA'bcտ{d\"<@FWH oJ[ {Kv c&[KYgW "6Fr\$0>>=)S xx&KS! esdk{%": \qN4 9 q$W.#V(dȔ!ߟWp"Qnb&:2"³CH33{EٌЉeو0y1Vo,19e.D`v-eu׿m 2^G3ˆ bwKX=[x?\:^0OˋT2wh4VRrp+}ӎla2꧷TN[j ٥}L@\qR!@RTTM zZn1{eED.?pqM$n*]"s ( `o7n PV8naD{Ta4 wn9d٣7$#5f3LF1d2Ylvd6[lvf1f9S/pmޓ׊FcOM~AȰh۳rT)x+Nxt?B iBV$ǟ+ fM&f&Ŋ&t5<.)5o_w[?}|h4y ~Ϧs-5000g^_LxwݘwB/WVݝs?rÅ<=ݿ:TZc0S/ZOr/BTIKOf).4e²=bGYAuYTWN20000jFg=KDDO۝WTێf8OF̍Ht: $1۱ϒS̊1rQNbYU{ΒmF~^-Tg 4000SyZ İ?Fvj}MYGM Hugņ0Qz|ێfN\Us{5?/H$l#C|EkVc]ͣ'DMşL0º_;4]U'+nk}^s靅(6>"!+y{1c~ FvboZ ;t|n.Ճ)@DŒa)Ҵ0r3KpyIlu\9{nհ& NaV5 ^/yzt)el8.9$\>" ҡ̐'ltAI'CҎ2Z|i):5FwWsZ0$2HOYɃO-X4,k" ڲJP#DHԽe5_ܽjQ_]ݚ@M9Wn 6H}pɀ":?-$;z}CwsaY~nKYYOXp4K# ڷ0LG8!rU8] %GzSG[eʲ@46a-rTreaf rܛ64DY,Ih)Je#|P}ގ04w}MօlFN~Hz\L 7RR%X"̍Z8vѾ_r) IleX5l$SZM\سc|u N/ˈ7eow;N :z| K 6~uh0ħFkYrN82WҷY Ϯ])g52Z)Tw⢢͌'E DwG[Ev9"-J,6eDAt?ANY _ pA 3CKL3 6bLpA0 #b֌}Ȍu$7ib ͵Ԗd?~\aԻ] _)L%zIx\ө+fbW>7Y8bψv=R9n<,R7n5yy3ו|ub%gnǥs$dmױ'D<Y )Zo\7C(Q3 'JiA AS?~i+:A{@@P՚_fҔ9WE֚ݿbK G& `FDqa xb$yub{g"˴}@Y]_ǠC^șH kM4;>y:8AH z]tIMdgfE+wc k ? KGُ3+.Y *mjCd6l;uɏ#L 7|5Uk2B샤g A`=R'~*+*2. '6}@e6!neZazvo>ՖWjHlO97Mq6[|{7K'NȥGGl54;?xD+_N{ bß}z)峅ѵW;F \m(w exg)*K}|p?]?<=o5Uh'-\>( `{ϖÆuqe痹'F۬ 珈 zmk(ln. A| <47^" Aɩ{Ϲ3וW*=‚dnٶ釖 pСE$^Q*6R, xxsx{ O[fpzKI[@A|Vħ7//9{1yȥgׂ9|/'q9b}}FY|{YZʙk[_I#ua] yav )ƥ|;222r;Y^vS~Z@_O!~iA Q7^yJ}I9t!/M yޑd106$8҉Af#"x3v+ry;lΓƂg4<}\}tӿOSRvAQR[is.T iܨ.kNkcM@l_YYeSC5tD?0` ,{pIp~O16kQ"` zm|}m CrjqbVRo ^W66LS[Ἳg)'r֗Z{ ϛ yOB!St/6^{3K;:/Pl46!M|Ih5(\ ˇf AĠr\3u8) e4N_72M.(wA鉵ן=ȀD0(1PZ P\qǫKՠ:7!5Eϋ ^QR\6^r >A+!nc來%C'BBČ,i9"4K"MF,6' \Jsٖ)s ZZS`p O%tKy| Xv;Xj/"=U"l{U?gfKڦTB5EU+^EQT*ظW,( (EtBI(!$6ew3?6,'3sN6<3w>msv:PY蜡В׃}'Ǒ3^T<16bքj<]W_?2Ii6@162L̓':{\zw~=[XXXX>Bt+Gpua mSHJ@MM!) NS!}Rv]9z o޼yb˓U^_YJٹCSp,;f~V?3_.|5\|r7G^D-OĒZr3ftOeU6BEf 6:Sb/!Mo-.^8B h}c˖-{᧛k?yq9XB^Nt{Fn)Q8`:XgKG3ZĻ~/!u+=UW[ {MgoBpo\ITųyê7p̹{֞ybx態FE}ST ;*|͘ sX" V/`H]F9S #y;u^Z};LMU7˯CR6n3hgU3m#7+2ő'LKݸ~ᘿ!Jo°!rcA~i@rL8zGm0J֚HP{Y]I'ItLGdȂbSˊ\CR1̣iODPqW' ^K߁ReϚ_a6W0/J }E֪+^2FܱwGlԀi.A/k)=s[GOu$ZӒy)Zg~ }dFbhzʑS"25~e'uvT,iK+Lwjis4"”{WkG6LvZVIw7wz3x߼=[xރƯH v|Ʋ qďc@$ IL멼Rz+$UG*sN>S$U<4rGIFI6}1iAze(~U]6V[_[]{#''''' /aQJIhl9\>ʨcGw_N_aӉe9gϝi \]3jmUz}T*8PַQJ)p5V^;/#_/:|$'''¢}e7ACGɅڼڠ JPflײ[qzJYQZHb OGaHS)Y>s˶]^jQ٫soXOLO\عLN3hCK'J>q)x,ҍ> 'SqOMOXܺ՜怑ϭy,ß<+UIs2?2s`=+/҇֡12^FB<ҳ/#kF-7;q&I.=z{ x# V /19wֻ}a{õ^SN30W8tǻ坞UG^QؑOy\K_IE?Z6 y_Gia+e-q:+e2?!8S7DfOCH1xb3 B$z'C,68,[뛫z凫ڗkw y $NIgs-]7K{nO~' ]AL E"@@QCE EbQ*x ݄=3HrRޙw[;nΤC޻ȮwySؘqX8O|lrzdTTfο.@'QQQQQ-:)]Sm q1 ұ\Kɉ>wwZAtTTTTٽf;7 8rjU]p~Aנ鮙AK]ޏWgQswȵ7v@ho6X e70>f](U˅EAQjp VU|&NvVTիN]L@TZ[2qG@_DBB]6ckcSoF+vnU78H\&me3uB9l|jb!;݉ȽR$U8C`?ʙWH ׆s~6~G۫u$ DR'k0KKƙA!(ݶy1?KغUw<=ZC^w>.Ϭ1xmMEq[GCĽE,Ahmʧ~mݸ }+hw58;lڻqJxc6&ȱ FMOy5 MUgw~ خ ~zk xg a5単:7wkΐ=Ol9SOK%֝Xi^1>vWE/j|mH,:>W˚N}ޔBLK;rDڹ#@#FO\ȯ~6b@&QK]gOZΞ0QPY[r@Qs~5^ƔgR)߯\S4me+@o;-r('p[OɮU OdظWor:/Td~᯺بʋjUybv^M.S_o.-;7e ;_A[3vQCqyemMf;}n M36SٲP蝉 ~rѼ݇vr^' ѽa~u4?Me_tW<ĕMVI<0彗96֯HVKk1sLP>`xrV'Јؚ,\);Xf(Taa /)RXm'6JyB,6JCK`VN^Q#b+- ,DO`YTv^ݹtڳXuG=zb^YTv뙬3,43trcvG~yyLxzQu۷?IYɉc_U9hZc?d[#!-7O-)W`/:f߽ c޸ڴ$u=cEά=Hot*jM M1|6xr $Q~G2Kѹ<!tM dG69l+^Bŷ\ B\X./*@#0]̬ή~0i=W_?kˑ?f(5I"7;xzΝ=C_r;[zy L`Zskuta޶7>x3Wo-{^!e..5.#F(D+4Hb, g8]N^+Â(H, ;A6b so' 'g4ɡ1|ŀqRD Sš:m>(_7xOLҨ@MdMEwyzyOڲabo4c6>}]8uw& $7]y t\ر*=v;M&`Ƞxrvju)#H>ĞB7t^%ut5] OW7ߠ9"$ j6f}F̙C_ 6|wn{㒷sLb0C&$3yzrp@K jҦcw|ek0Cn׳7i+8rK0:SsHBG Œޏ$ _<ٖ"F3zTyéѣiBgGXw*#Bˏ+ꄡ,X8VvZ~sl ٚ8W0S~g3H!遗xʓ{xfWp\囫@ aH̓H5?'bڼj??@HEa(sOOϴM') @E"B\xb[IT]]y!ȴ j10lUM>'K(o+7ł–Bڞ8n_ ZjJXUcRz|ErB[3 l׷@Kc+д` 0W^L] } oӟ.*/Š+̙7'V儤=(az/Jc$b-H䰗;" <2@)Sa2:RTrg9ނp[EMO$,hzT "Et^;76QЏdHqgr:5L.]QE!q~'^b.i D#v9ljw(pYQ@nSp2逕'wu _`7/]nN(/| ċÆ$Jn }c׬AAvm{{==>PNă@GS^&bb_P(J=zʫ#0T1 Ox=@GegE]W[V QgsGIU{Us}ַ?n~j20\n+hnrw<6 o}Qb)DϢU.3s( º̯;]Y|Xa{=hʢax,:wjRBdG%\s|qjѮ?^j?njuAVHVgYձrؘ8d '3v]c^7]ckƢiA7q4#<71>Gt1>])l"6AI.SR4L>'(&Z%J KK W1IRH }#*`L*ScG{hΰXTh|&*Mxt||m O es T׬#BqC'H O)t5L:w."'tM"|R P@ Wi{!ʠ`T"OIJТ.\Np;AkKTɣ3yhvt&VT~յR;"¨#:ϕcqyj/RTG߿xN5ws`<;zpnJ.AH> ~KϮe3^nj< v~p8:޿Z7_ϑ*.W4-\3FΏζ+ïis]馸.N}mDқ B^ȉ q.)iBd)n!Ǘ- EJ5E&ũ 46ib%{ö`NE䑃]\ o>p͙cUj??*ɃҪB EQP{׾.䣃#anWQ~^Mue>JнE~*]ƺAS?X}3GJG8$rI 6Wqm9 !Hpw#]wڭ֪7`IMd1zKяL܈uOy Wu;[R[Uy@@$zKqT5pA" Sģ`_j!^a !:B"NdΩ'Pjn>>Ց[-r#737T?rPH$SYxRStPVt)5upi{=ɽwXp[kD;0^wy@l%8x]#&/\٠@ W}-V?I t3$R=El8;)9aV'6&/Us ' O4biO՘Kp!b#@g4$C; "J+ PJ!@tw( GhQ 9 {'zG^-EQu9]25@h.r,ʘ=vmCvdPRA#?EQ'^>+fjW .9鉁!0Mz6x.asg'9ѹ` Tll/m) l\Uh魵\ӇNZ?<^s*kQ??nB|d V3bCH7%"% q <-H[Ma`Dl(

K]?YlF>a <2A,TQAo @!@Jp_y:mNʈ#_`썉\Q[.H/!ӖXM9fxQӭ-w}ozن%݂8 nSۧʅ pXzGܛ"S`{wrma_Sػ#P\e#/bEY®o<=D#,xv wA#v";gI`kpiڎ[l?WÃpZmS ) 5g|K&/xX 短 ;\aF\hWeAtre(EI\/:\| ^FѸ!%Kn\'IG?'(\%| '>9 *b)*mQ'$K7 ɰ1BD%^|Zte%E IT\m皢(45Vjm(:.@NHx9WWXԩi.lM7s-.w|`-QN[/i1Ǫt7UUPh"R|8 NW0pɞgoT4J (6<[bTE$O  `Qmw>d#(`5TW&$QAr!io\.jJ ?>)ܞxwg\,2xJ#bl[O5ŏEWڐgK{iFG(axY'Ҁg,W/%kf){9irA0-$.@ .]ko6\7p~"$.z(hWW@M6' 87\a\qA1t|"&pU0vu?M݀# mZsP>0?u6ap5 #cTe֠O4RR{)Ǡ4\/3 iŠq0k X H kCujYQ7ڃ̜/T$6Si/+$a!dg_.7:z:GD(ڊSs `:,H"4qg)iy<`j T&\nۻԷH\dYWD}fiY[SR/J t%iy ^6:Keb \|Cظ$xl^{A=}>u;U[f(9vR`w̋['p-ǮK5eZP7.A{ C|߶?[N'ġמ:[iǃBlמ_lRT}I7{~խq}cuǷ5/@|)x]MF$y@^% =Yh.<5OAv2(I[ڊ]-Uдc7^+--lV}A4边âT20|6Qtu\WkҰ0_}!roj2VuRDm=#~1̅ffJFS鞏_6hdO{s^ᢺǟ{po?!% JO2헹G7l2'Q$ Ez\'{jqJK3r Ѱ( /74`'W '2~i?i/RXk(jnХnY꛾`i:;LRSm"K b,foNn1>a~~&F57Zar+5=x }!BjW) tuYEO@r7;=3x`#l d\@TRD$ tfԇVtb J W}F`x&E; 썷d.Z"TM |o#Di1_??XL?]Ѭqre6j ԇ{Gƅ3qK5\-7~zF=|e؁˵?丂vnr1_9}EdMeQ%c,馴>2#GKߪm4w8.cm)oDxxsP7n/-o5E.LjukT۶}yq-w?͢]]|dr{_jYm/=YX%S+H|:;/7<<٣(LgY2z?_׷Y }52lhkmh-zbſ^ـQ3DS{?ArqKKpxmfcQUk064קHx!?յkPddn4e !D2߀Psjff>bAE)Q32! b$*BLׯ&UŻ׬ˎyQqrxb":)CX :-LgQNo#eу }ϙcJB|^#y&z' yZB04EWVHbB+ˮGҳB88c7gUaب3'*P#$0uB63؟H89[xCvah! !xC,m.CJ TJ(t@$+J?_&hBTV b46<½ڦBzC.--[?\}TvpXAc}0W遬rT6jtи@1}Nkj:__12AF}15T##zdž@_Qܘ:qDD8;4nZ }("RK8V{ZPe׫*uU{!] o-~)bB#DDV9rP( T:j[fQ $&5W bH H8;q"_.C9w%j+KM 7V9;WGMZ[е,pm/s}s@3/ySd$>tSPUiNv]. 7kU>*%EQ5eg ^_W?!*aLgҒ%ʦPLJQjl,$jݘo\}dƍW W7F&vrQ*^J*'jn䶶GKu rAUi^/S6(UciijuH z翎p8pk CǍN 2O,_#;Z-,{D$7}xV?<1Up:Up\6rE`"(2HӃE[jy5wPW~?Y;}kbJ_ 5r)B2zK 6lI+.,ݞ')4VG ~YF}vJA#V}:12T2nlRΖ\/ z3-*cm!c1X l_py5PlM]cLk[m+{%BD>h5/ag1/O{aۿ30I}Ce[Y DŎ7,ȧP A `ѭ<[6&}}U8H%[젦K}nP+>žm3Ae맍28>ĔT_k#cN~v\@ʯ\_u1r!h 5Q#Ey.[|R@suIkPWYe^AiB?yqkq(R yG'*(%W$r?~~iO~x#mj퟈@XJKEDBxX mQw]YRج|졠o];ro0(e/ܓ h2QpH D3 eJQɿF W5961wlػw ; 7o|?U9jœR5?ł@^+cCߐBhYc@( Z {Z ST6%$=-/p$>ڈC -r$I4kPt9MUV2u`hxBḒM i@ttJ" 0VcS֢ V mZU HGcd!6"Tei147ֵo S :2$@.]WY۽a߮!2؁T,& xZUcͭ:CۚuVo@RJ9uʘ _2%z? pZ[Z V8ZkpZ*8 uBlr9RmTQV_ߤ2E$J\eUd!Ro)V6w\UxB\SjBߠ@Nrc Xr[ @RIxZLZ̥'Jj·[mĪJ&-e5B&:1p݉zɣqgxlzne⇖e`\H_1-{޻"F1S$w]4 xe= /Qf4\owNg"͕{[⁀;le ~$&#`"ؓl1[Md1QKFKceJbxrXYs[ʣܳo#dڎHɏ_>>.K9LfZ @,LvX, u:[an7 BXl6E"P(fY.Z[[vT*u8zB(Hfh B-lr[rI$& E"Q[[dJj"V(X.H(th,[mX)l6X6B6 0bmH$2 &I&QJncX0bG1͌X/Hl6[[[۳cbJJ$6V  0nfe2 R3 B&z1h4zx1" cz{d,lϺnzpu:0X.naxκ cH[La6֭YwζdzBlc≠Yxwܞe V<с(u S% x> 2xq '~\#lyDkaVqq4w’z!iyhʂ=iU__O ٻw/M|0:M'N`fڴi4M gΜ0̱ch~7yh޿?p޼y4M766vǍ!<Ÿ/Bϻem,0uuu4M/\c~!\f M'ON:m^w !?i~뭷<8pas4jƎ?as4/3 i&w1B2l0a>S-[4uVa{1 q0Lqq1Mӏ<0ncKs=Gtvv6pԨQnckkkBCG}0SN1 3eM&YfA=6B~z<!tdh0amի!7nt0̒%KhdclNNMӫVbm0ʕ+=0O> O4Mo޼a.]bfȑ c-Z!ܽ{^{ӧO{5:{cuyw=Ι3f1633W^yc] /<|pիWB+7n0 ӿ}B._Bm6>,Mӗ/_fSCkã*n˦lv6 )dS)JhJ*"("  " В@Bz/em?ٙ7 =8ws~w]Bv&g;~,pȑJUVw8k4Ϝ9T*?sN2&L@1bhLLL49+ʃ-**g,Yb4gF JGPg333J/lrv˖-FqҥB !,..ph4:tHT[իWFc\\!OpB_(ӧOC;ikkS*qqq&g?uLΘ1CT޹sGT.]B]|R!hi:cRkJHm.gF `ydPay&%t!$>$Z' Q6z:qSByl%lw͑.%1d{p4]7jCCU8I*ynn mYARk>4KnB"gEsFVȠ@Y&џd0BQ}.]ZozNrÓ[F1dlP=~/TBC?w\k5`xH sK|jRĦ?! s"Blʡ@d(.$RIaL$ =ƒU ¦j'JJRc<`Ʀ o8M]FQ4gǾ>BC:ރ1+GuӜ" ;r>7Ij˃}g2[^\Ie߫?|A/L`@ԻG~>nw%37iXc9CmYTa<qFKZ"TGZ7yj7z1BF{e?0:P5Hu@d6g>q6 /$I_|4|W] fP+ 5gNʸY="knÉec;)5aY0Sw|VQu`q7=5n(rT yOG1ǰʼnL:U+c۝ i9IVNx\um+ 8nԠ? )ŭb[SjBRK)ox>?qb>uW_m:uDCΝ?O-mO9IVs%S>K=nlS]~ NזxVvM}}XvD6p_>>;*zy;ƽ #bB"Čߡ3.!dhɈpd!5|"FLz/`!y@Oȑ a9Fuٻ%nƶo&,c#WԠ[6Q0iV#~,LmȩֵxuW8>vregpRҷ ~jKcOBI 6x5ԏwrCg\ o}ʛrnm ZW'J 4aTOFSybۖRmcN>XjAm]ڙ[?^rS7}#s us0ٴ}D=!V_:}Qos$!ԜNKT{wdlńًy #1u w/ed"GRHHHXl$fB˭L9!B9<ʭ5U߹|ؗxZ#  tM&OCa ҉";^I}v~ÆˬjRV]|kرcgh6kR?wρG =I^LaFc!|P͌CbM|?gr$b Cdi7s´I zU\4r!{hzpF1n˜1~3/HMj$zXiS&1JLN3ns$eյEw邰K&  1禩O6i҄&Q=LaF dŐ<P6m--Ը&RSI2t~h1ƨ^`hғ1ZLKSq̍tOW>b0?o ]݌޽[)@֮է:Y5MDa,NJuE]]ynw -Ǜ)rlv ݇]hs$2Tg@KK /,RUV]f0$'_R#{9IZԍx8_`5/l-yInK}IV mє_)cCZ Q)" #;p)fmMW'B5yn綄Nníנ`>Xa V[3mU>pa6Y \EPo_Y5ǚ)9㇤VoOV|=b`a{NšNlR=0 #Ϥ"5UE9 1m&hYNhh)|iw9 ȤR5ab_\ ]JoW&'ewS@UAFZe8rTe;:q)ͽc 1k0 Z"s_+uuʢ߈+ ^}=mmw*[v2VY9Q"ڋk`ܤ>+*c3RWu8TlZ}p,&ER%9p|mɺz\ԍlMⶍw^mm+'|=Ȼ|TiogyjF^"{y. hwyZ! 9ikU[x'ߞ8惬1ʳe9ښkKz А~/Q" nXɻszI]]Ga}ztu*[TWoM`؞2ߠo~1dr-X7y=`^f.+ny)@~MdgŃujUH,z%B@Anv!"+W{WcEcFjUQZ !~zOSw? ZM~AJ(s8{?"O+0UC M<3=S u lEe;v@*~𯳞_#uU_ر9M=ed-A EV2~ G&$tݽ3~@)%DY;FPZTeE>.`0 Fh,;oزsG/A\}oUo7mp(m^c%qKDuL7!MJ]3S]Vkk-wAsSZ$NnO^hFm:'/~d;!0%V ; NQGEWG w`Зv"B]bȁm$TU ||0r"Ər<⅏?h=G/I^kfQ̞ ># 0EmpPQP[[Vv=ںv^~,mjtƺɺV*fBMgIc "Yx$V jJ$Y|D\Y|xȐχ BMLc0ZZsj|H[|ANvϽC?76[Y/r+WxasWk;t\.GO ɉ{0aʊ&>ؓmgpcP*(V|>c4U e)S7D.3Wh~x/[d'fPR\h;,"X<{ό/ %c|Uqh\FS&KΑ BSUuU.PD|q U[#O 3rTlezA|6rQolmY=+{F()wW'm)ZK$^*9d+Q($vUV{y/{*c_\sl碟a/g&?_4GsΞ߯j&]@D,ߋ\z -[w[ճa ͑`~/NВIGt혡.\2/-b޺5zBm}iΝ8;\BcK-Ku tV=$-C-+{ygrloä>sQ֨;7~]B#XD%ѡ^cT/ϐ'lM_a*oaQKO@ (#l l!QY %I%FdC )I9b9$ryЀy @z ˦QA^U/h%b`~ę:^zH[g|G*x@{Cyi ̳b]kk_tP}W'gS"U> !csvya?-vɿFu iWmήPru_R{,زxl8h0x7FE}_9R#BՀ"5HTGH  [ {"yRy1V,9-tTK9J1M.kT$r}@DTh>iQAG:q@X@Հo7BhPm]&M ɵi !}hDc@RL0L"8x,XIJL~pr˗/?qsל(o|dc['Z 8:3_[Ҧ [.q.uCaV̓ 1zx+/-iCsXS[m]7zr+N5Jr/ sw,׀=H9-dl`O,3&3M<2]9L$FO .iX0ld>s̰ØS*:x.L62ƼK>[ RۓI-مQcC!gA<[/%-<`{35CнHHT00ZȞFuJ[ŬS˖YlFG63lYK$ 0)y|`*V) Baȑb]b#W<3dVCylS 5G#rh IvHɼ'P &lH$I *9}~gvxH䎌0# B0m7]2rUڔAJYrmU3#GŇ{Hw^^|iٴ甝t %Uu"c l{Z-o)<\'K%Ée,@ldUEƚ9OŨ U  iHE`O1&UĚL&쒴Id!Ũi+2ve" Ek,T[a6ԅk) rդo:JlTlQn7HXHقEdK#2OjGz*Ycx jHol}5<#{Yuk+I[ݢ)|\|O_ ŏ(!l(JI2䟸<"W"`2 4&d#kK#Ӄ)%a3&H.Qa.wV]XbcqF;G[7n%llP !qT":,Á.\H|TH@w!K(&=M, !`aPe8foI]Zp!&y \;ܓO 벢ȹY88of k$!@e(gg蹤.% X sd ٟUl`y, KlE4H;sXGxj's]i@}SޞlT޶^=Sy(Vi0Ϥ1a}խXvQc~I}T@M`.,$j}fc>?   r GOlG3 lH:gtYg;~W!μѱ/mSB3Jޒt\%BCIK4Pu +٤ LEܻւ?U$Ч f~y* F&d {D-˴o.PXqn'C^|NA.!~bb)Rpc/L}oa@7?;dȐacߒ@:Ժccccc'ߙMfa F%EYba;3d+ܫKkޙA Nz@& f:0װ5LHa$0o:q{mR/6+7juƦs &vJyҵf:111LFg<9ii?٦5"s7WEaTd-j#Pi20 F\pl4ȮC.܋ᓻmQV-rه{9'[p %Z(`byR]A<ע=ػ8Ys跄'Oݢ-8;8Ma]smNL~yl={Ǿ*F&G%L''!̽_;o>hc7unr˫p-E>mj1|}^uB<~`sCҗ1Ȗ*/O7v6fnxTz /NX%\~ϟnne :Jd|M_ _pњdY J"Fw5ra{#?]Ja:ZL +/U /߾ꝏΉV(K6*lSG]~޺!/-ғF) "|ɵٶnUK& [q@-j`/ bƏ @s^(ƽāg/t淆DN>Z[~ m!hHyB=yٞ;uݪ;~e bsK-D{DM>VTBg`Hа!r[V[I~6нᩆ%+Z[ψ7XwhxM?0lXp]M} P_vE߇}}Ƌ诅>|31 2@TݠB^xdhۤV5+Rݾ|ވU(^+Р:B3~G[K6>>zswvhWoբQ{*n6>G>CՓT,Z:s#C 9{J٣^|֏L^?y)(x#W\m[AlB  uw;6S A[ʘjDq l/]z!4LzuNlXXXX?O'k@!C}$;F1yT޸T9z[Epd߰Сږ6sFnu. GB*>"@aZғqY d㷤"+Z6i۷l/S_YówM݉ff߻~|զߝ9h%#j_~ڽ5kkQo_wC3 ^WM~x`QSٹ]]+OYnX\ au+VvG[NmfyyQNLt$`=i5P1IOAW C dɛCbbLܚ}R14.wݽ|!xҢ*؜z?r  LO9$tC?0 ZнAXއhn9^Gnv&Ξ-&-qr$Af?6Mz G{e_a^\դ'_=D778ɀ  ?radog41%R2+*R.?z>v玾5Wx/Nd'\='LjNuwυ/ zo,Ӓ\B KHyae_@UV{Ce2ro;U`xHr:o,Oi@Pus?|u篖Gm'M[A̽׀7u**iM$L'7ITFaj[ Vi/.x{٤0tsYʢ'GX[wl,3475 .~~/ϞMbUqصkWL6"?*л><KEGxI!ZUͽX\?5α=kދJA=BU%?y)% rśdc'1*C'kCE,͔|"H@T cdrHk*:ܓ+:;{x7'g\wvr1 S\ Uw~%Je)i}u3u:pfh| 㓤Zda&j@gggI_ɮ9f}v_];0ڠi,i5ïTȝ_[N{Np2c:M~aQs3%Φ/ Д4ɇǛdR!4?0gV]j.ږ?hsp@F* 4ueXpƊ@a֖jܰ|DsK,MQ[x`w$x=FOT_Kl5<ɴA6IhyLjRGKSvM=ؽdcO@KiI 44\߱H =V jRE.Ol/w6MS}Uar?qmcA";&k 6(fKTz#]*뗏1>>0.Pը < vA!8<6g-;~b>@C{mVNAhuqjiаiW^v"0?;MףZeɸQBuU5vW7gO>~V?(|x<wh7,7B#0X1I 692O^F*(037Rѫn֖^wζS[ ]B؞@#t,2n<6N(-*tH w1$mQY"RaS5`A`l \Z AN {<'-׿bӴ'rmoӻݽEw/6C_u|?>_]:u$7!lW@9<&o s3k Y^ӯ93**jت{^S_^<,$O;|P4^66V߾䯐?v緵úԻN"]edC,Ibmê7k#VUPRflST5K)c 1hkFW|!r6kCyF`#y] u &G)ŠKX<2mMi|k$ǂ uCs&uswƮ;iY-l5t.JBǎAQ;10O @Ǟr2 0,ԣ!GR. ^V$R:AXZ9HR5b |Ms*KD2)2BPk/O?q EN&nNR mu  Ӕ6E){ȤF~oʠt 7f>HJ^1ݪ8I"lôf1S JCl 9agВVȮIjl#k: n[AP.fgyV+[@7`d]`-ax<^>^ ? {P_g7 Ogny3_ JEdi?Ѧ8#N /Z|w8ۥ3z;EM*kuUg:1d2^>_oIrRs]2_}= .(u0LÔ{3Z2!ЩK4Fw7gA,8y("T"ԄOOSs |[CL Q]_ ASsÅ/']P۬V79{pa͚q C4jZ "Q ;U`a3X1mvlك <*j2uwmTb0m-uFUƭĻb>y9c_-\zTwJ@[♣6=^@kз՗Oy qϋ_56O.IF-.ZemO\Xs|ء)au^ })M 0 t%3o'޲ Yߺtbo% z#!5/}IPdb.,edl⿺;k5M\]e/~Aǚs}yqՕ+? sD 0wpvW\qeZd792Adt<5)%Teņ~]ŭu%iHDxOw^\x7Raq=S#  v p6wVl7Q[}M(x)Cziv^sãƅlCfbƊy%]'-}/ec"W{Xytߕ,~ss@2P|IIII1ÃdrM2FܽO]c^_Mo((DXCy-IؔA>2_vѤf i}VpuũS<{}k-=XT{vX,*XՠłIc0"V-m灤VQ/߫c|ULZJ.U:>V~r?t2zx70Q is2u-~2hQ}%S4G z ̳slD2?!n$]8 jE.I>ż =bD6`n>i$-$9`PVHKĚ\j:QzZG Gs5Ć{-5INohͺqA݈DtINJUk'CM3$wߪA̜%^EIs]f8![E]6r (~Tĝu֮w;ǿ1k֬(t6Q{{ ǿo '^-%_ɹ5XSn%zj-$ U`,yܝgK8diAKlor?[@Ϩɖ]OqMEl-V_r(aA-){y1QUx6Ae㑚}[a-HTI@CdBI1@MbL`> endobj 366 0 obj << /Length 30217 >> stream 0.271 0.267 0.267 rg q 15.000 44.491 577.500 732.509 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(These experiments indicate age- and genotype-dependent apathy or incentive motivation deficits in a mixed fixed/progressive-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD, a full-length transgenic model generated by )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(William Yang )] TJ ET 0.267 0.267 0.267 rg BT 85.842 745.174 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 95.479 743.667 Td /F1 9.8 Tf [(, and the z_Q175 KI model derived at Psychogenics )] TJ ET 0.267 0.267 0.267 rg BT 322.557 745.174 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 332.194 743.667 Td /F1 9.8 Tf [( from the CAG140 KI model originated in the laboratory )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(of Dr. Zeitlin )] TJ ET 0.267 0.267 0.267 rg BT 81.513 733.269 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 91.150 731.762 Td /F1 9.8 Tf [(. Specifically, the BAC Tg mice exhibit deficits in the number of rewards obtained during PR training at both ages )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(tested compared to their WT controls. However, under the mixed FR/PR schedule, the older BAC Tg mice exhibited decreased )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(reinforcers earned, and decreased response rates at high ratio requirements in the PR component, with no observed )] TJ ET BT 26.250 696.048 Td /F1 9.8 Tf [(differences from WT controls in the FR component. By contrast, the younger BAC Tg mice exhibited only a trend \(p < 0.08\) )] TJ ET BT 26.250 684.143 Td /F1 9.8 Tf [(toward a significant decrease in response rate in the PR schedule. Thus, with increasing age, BAC HD mice exhibit the )] TJ ET BT 26.250 672.238 Td /F1 9.8 Tf [(emergence of a selective deficit in progressive-ratio versus fixed-ratio responding for a potent reinforcer \(evaporated milk\), )] TJ ET BT 26.250 660.333 Td /F1 9.8 Tf [(when data are expressed either as reinforcers earned or response rate at specific response ratios. By contrast, the younger )] TJ ET BT 26.250 648.429 Td /F1 9.8 Tf [(z_Q175 KI mice appear to reveal a more severe phenotype than do the BAC HD mice, with moderately decreased performance )] TJ ET BT 26.250 636.524 Td /F1 9.8 Tf [(at all phases of testing \(FR and PR schedules\), whether data were expressed as reinforcers earned or response rate per ratio, )] TJ ET BT 26.250 624.619 Td /F1 9.8 Tf [(compared to WT controls. Moreover, homozygous mice from this line were not able to acquire the basic lever pressing )] TJ ET BT 26.250 612.714 Td /F1 9.8 Tf [(response. In preliminary data from a small test cohort \(n=4-5\), older z_Q175 KI appeared to exhibit a less obvious phenotype. A )] TJ ET BT 26.250 600.810 Td /F1 9.8 Tf [(significant effect of genotype was observed only for the response rate in the FR component of the mixed FR/PR test procedure, )] TJ ET BT 26.250 588.905 Td /F1 9.8 Tf [(possibly due to the reduced level of responding in the WT controls \(i.e. a ceiling effect\). Based on this pattern of results, it may )] TJ ET BT 26.250 577.000 Td /F1 9.8 Tf [(be that the aged BAC Tg mice exhibit a selective deficit in motivation that is due to incentive motivation \(less goal directed )] TJ ET BT 26.250 565.095 Td /F1 9.8 Tf [(behavior or less wanting\) rather than hedonic motivation \(less pleasure derived from reward, or less liking\). Young z_Q175 )] TJ ET BT 26.250 553.191 Td /F1 9.8 Tf [(KI mice may exhibit a more profound deficit, involving both incentive and hedonic motivation, resulting in both FR and PR )] TJ ET BT 26.250 541.286 Td /F1 9.8 Tf [(deficits. Older z_Q175 KI and WT controls exhibited much lower levels of responding overall, reducing the opportunity to assess )] TJ ET BT 26.250 529.381 Td /F1 9.8 Tf [(genotype effects on motivation.)] TJ ET BT 26.250 509.976 Td /F1 9.8 Tf [(It is generally the case that these mice respond slightly more rapidly in the intermediate phase of the PR \(requiring ~30 )] TJ ET BT 26.250 498.072 Td /F1 9.8 Tf [(responses per reinforcer\) than during the FR5 periods, raising the possibility that these data could be influenced by some motor )] TJ ET BT 26.250 486.167 Td /F1 9.8 Tf [(issue preventing the HD mice from matching WT controls at these ratios. In Experiments 1A and 1B, it is clear from inspection )] TJ ET BT 26.250 474.262 Td /F1 9.8 Tf [(of these data that the underlying cause of the genotype differences in these experiments is related to a decline in response rate )] TJ ET BT 26.250 462.357 Td /F1 9.8 Tf [(in the HD animals as the ratios increase, rather than simply to a failure to match increased responding in the WTs. In )] TJ ET BT 26.250 450.453 Td /F1 9.8 Tf [(conjunction with the apparent ability of the BAC Tg mice to maintain their responding during the FR5 periods, it does not appear )] TJ ET BT 26.250 438.548 Td /F1 9.8 Tf [(that a simple motor phenotype could be responsible for these results. In the z_Q175 KI mice tested in Experiment 2B, it similarly )] TJ ET BT 26.250 426.643 Td /F1 9.8 Tf [(does not appear that simple motor deficits could explain these results. Although the z_Q175 mice responded more slowly than )] TJ ET BT 26.250 414.738 Td /F1 9.8 Tf [(WT controls at all times, they are still able to raise their response rate to ~40 RPM during the early part of the progressive ratio, )] TJ ET BT 26.250 402.834 Td /F1 9.8 Tf [(suggesting that their relatively slow responding in the FR5 period cannot be simply ascribed to an inability to respond faster; )] TJ ET BT 26.250 390.929 Td /F1 9.8 Tf [(more generally, as z_Q175 around 26 weeks of age show only mild hypoactivity )] TJ ET 0.267 0.267 0.267 rg BT 373.067 392.436 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 382.705 390.929 Td /F1 9.8 Tf [(, it is reasonable to assume that motor )] TJ ET BT 26.250 379.024 Td /F1 9.8 Tf [(deficits are not confounding this assessment of motivational state. Therefore, it is most likely that the observed deficits in )] TJ ET BT 26.250 367.119 Td /F1 9.8 Tf [(responding in these experiments do indeed reflect the pattern of motivation abnormalities outlined above, with only the aged )] TJ ET BT 26.250 355.215 Td /F1 9.8 Tf [(z_Q175 KI mice \(and their WT control subjects\) appearing to exhibit a motor deficit in terms of decreased maximum response )] TJ ET BT 26.250 343.310 Td /F1 9.8 Tf [(rate under both schedules of reinforcement, compared to all other mouse strains/ages.)] TJ ET BT 26.250 323.905 Td /F1 9.8 Tf [(Typically, progressive ratio studies in the literature report performance in terms of break points, recording the ratio requirement )] TJ ET BT 26.250 312.000 Td /F1 9.8 Tf [(at which the animals stop responding for a period of time as a measure of the maximum effort the subjects will impart )] TJ ET 0.267 0.267 0.267 rg BT 532.909 313.508 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 542.546 312.000 Td /F1 9.8 Tf [(. In the )] TJ ET BT 26.250 300.096 Td /F1 9.8 Tf [(present studies, likely as a consequence of the training procedure employed, the test animals failed to exhibit clear breakpoints )] TJ ET BT 26.250 288.191 Td /F1 9.8 Tf [(within the allowed intervals, continuing to make intermittent responses for long periods of time. Consequently, the approach )] TJ ET BT 26.250 276.286 Td /F1 9.8 Tf [(described here was implemented instead, measuring their work-rate per ratio requirement.)] TJ ET BT 26.250 256.881 Td /F1 9.8 Tf [(As has been described elsewhere )] TJ ET 0.267 0.267 0.267 rg BT 175.279 258.389 Td /F4 8.7 Tf [(22)] TJ ET 0.271 0.267 0.267 rg BT 184.916 256.881 Td /F1 9.8 Tf [(, operant tasks generally offer a very valuable tool for assessment of transgenic animals )] TJ ET BT 26.250 244.977 Td /F1 9.8 Tf [(generally and HD mouse models specifically, providing a reasonably high throughput, largely automated and sensitive set of )] TJ ET BT 26.250 233.072 Td /F1 9.8 Tf [(tools to assess these animals. This procedure, requiring only a lever press and a food magazine, is ideally suited to preclinical )] TJ ET BT 26.250 221.167 Td /F1 9.8 Tf [(screening in any facility with a simple operant setup and will ideally provide a helpful platform for preclinical screening to )] TJ ET BT 26.250 209.262 Td /F1 9.8 Tf [(ameliorate or prevent the emergence of motivation issues in HD patients.)] TJ ET BT 26.250 189.858 Td /F1 9.8 Tf [(In summary, the present data provide evidence for age- and genotype-dependent apathy/amotivational deficits in a mixed fixed-)] TJ ET BT 26.250 177.953 Td /F1 9.8 Tf [(/progressive-ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD full-length transgenic model )] TJ ET BT 26.250 166.048 Td /F1 9.8 Tf [(generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 145.463 167.555 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 155.101 166.048 Td /F1 9.8 Tf [( and the z_Q175 KI model )] TJ ET 0.267 0.267 0.267 rg BT 271.087 167.555 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 280.724 166.048 Td /F1 9.8 Tf [(. These data extend the range of HD characteristics currently )] TJ ET BT 26.250 154.143 Td /F1 9.8 Tf [(modeled in preclinical subjects, and may provide a valuable screening tool for novel HD therapeutics.)] TJ ET BT 26.250 117.541 Td /F4 12.0 Tf [(Competing Interest Statement)] TJ ET BT 26.250 97.587 Td /F1 9.8 Tf [(CHDI Foundation provides financial support for PLoS Currents: Huntington Disease. Editorial responsibility for all content )] TJ ET BT 26.250 85.682 Td /F1 9.8 Tf [(remains entirely within the remit of the Public Library of Science, the Editors, and Board of Reviewers.)] TJ ET BT 26.250 66.277 Td /F1 9.8 Tf [(Stephen Oakeshott, Russell Port, Judy Watson-Johnson, Jason Berger, Jane Sutphen, Neil E. Paterson, Sylvie Ramboz and )] TJ ET BT 26.250 54.372 Td /F1 9.8 Tf [(Dani Brunner are or were all employed by PsychoGenics, Inc., a for-profit institution.)] TJ ET Q q 15.000 44.491 577.500 732.509 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(These experiments indicate age- and genotype-dependent apathy or incentive motivation deficits in a mixed fixed/progressive-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD, a full-length transgenic model generated by )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(William Yang )] TJ ET 0.267 0.267 0.267 rg BT 85.842 745.174 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 95.479 743.667 Td /F1 9.8 Tf [(, and the z_Q175 KI model derived at Psychogenics )] TJ ET 0.267 0.267 0.267 rg BT 322.557 745.174 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 332.194 743.667 Td /F1 9.8 Tf [( from the CAG140 KI model originated in the laboratory )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(of Dr. Zeitlin )] TJ ET 0.267 0.267 0.267 rg BT 81.513 733.269 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 91.150 731.762 Td /F1 9.8 Tf [(. Specifically, the BAC Tg mice exhibit deficits in the number of rewards obtained during PR training at both ages )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(tested compared to their WT controls. However, under the mixed FR/PR schedule, the older BAC Tg mice exhibited decreased )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(reinforcers earned, and decreased response rates at high ratio requirements in the PR component, with no observed )] TJ ET BT 26.250 696.048 Td /F1 9.8 Tf [(differences from WT controls in the FR component. By contrast, the younger BAC Tg mice exhibited only a trend \(p < 0.08\) )] TJ ET BT 26.250 684.143 Td /F1 9.8 Tf [(toward a significant decrease in response rate in the PR schedule. Thus, with increasing age, BAC HD mice exhibit the )] TJ ET BT 26.250 672.238 Td /F1 9.8 Tf [(emergence of a selective deficit in progressive-ratio versus fixed-ratio responding for a potent reinforcer \(evaporated milk\), )] TJ ET BT 26.250 660.333 Td /F1 9.8 Tf [(when data are expressed either as reinforcers earned or response rate at specific response ratios. By contrast, the younger )] TJ ET BT 26.250 648.429 Td /F1 9.8 Tf [(z_Q175 KI mice appear to reveal a more severe phenotype than do the BAC HD mice, with moderately decreased performance )] TJ ET BT 26.250 636.524 Td /F1 9.8 Tf [(at all phases of testing \(FR and PR schedules\), whether data were expressed as reinforcers earned or response rate per ratio, )] TJ ET BT 26.250 624.619 Td /F1 9.8 Tf [(compared to WT controls. Moreover, homozygous mice from this line were not able to acquire the basic lever pressing )] TJ ET BT 26.250 612.714 Td /F1 9.8 Tf [(response. In preliminary data from a small test cohort \(n=4-5\), older z_Q175 KI appeared to exhibit a less obvious phenotype. A )] TJ ET BT 26.250 600.810 Td /F1 9.8 Tf [(significant effect of genotype was observed only for the response rate in the FR component of the mixed FR/PR test procedure, )] TJ ET BT 26.250 588.905 Td /F1 9.8 Tf [(possibly due to the reduced level of responding in the WT controls \(i.e. a ceiling effect\). Based on this pattern of results, it may )] TJ ET BT 26.250 577.000 Td /F1 9.8 Tf [(be that the aged BAC Tg mice exhibit a selective deficit in motivation that is due to incentive motivation \(less goal directed )] TJ ET BT 26.250 565.095 Td /F1 9.8 Tf [(behavior or less wanting\) rather than hedonic motivation \(less pleasure derived from reward, or less liking\). Young z_Q175 )] TJ ET BT 26.250 553.191 Td /F1 9.8 Tf [(KI mice may exhibit a more profound deficit, involving both incentive and hedonic motivation, resulting in both FR and PR )] TJ ET BT 26.250 541.286 Td /F1 9.8 Tf [(deficits. Older z_Q175 KI and WT controls exhibited much lower levels of responding overall, reducing the opportunity to assess )] TJ ET BT 26.250 529.381 Td /F1 9.8 Tf [(genotype effects on motivation.)] TJ ET BT 26.250 509.976 Td /F1 9.8 Tf [(It is generally the case that these mice respond slightly more rapidly in the intermediate phase of the PR \(requiring ~30 )] TJ ET BT 26.250 498.072 Td /F1 9.8 Tf [(responses per reinforcer\) than during the FR5 periods, raising the possibility that these data could be influenced by some motor )] TJ ET BT 26.250 486.167 Td /F1 9.8 Tf [(issue preventing the HD mice from matching WT controls at these ratios. In Experiments 1A and 1B, it is clear from inspection )] TJ ET BT 26.250 474.262 Td /F1 9.8 Tf [(of these data that the underlying cause of the genotype differences in these experiments is related to a decline in response rate )] TJ ET BT 26.250 462.357 Td /F1 9.8 Tf [(in the HD animals as the ratios increase, rather than simply to a failure to match increased responding in the WTs. In )] TJ ET BT 26.250 450.453 Td /F1 9.8 Tf [(conjunction with the apparent ability of the BAC Tg mice to maintain their responding during the FR5 periods, it does not appear )] TJ ET BT 26.250 438.548 Td /F1 9.8 Tf [(that a simple motor phenotype could be responsible for these results. In the z_Q175 KI mice tested in Experiment 2B, it similarly )] TJ ET BT 26.250 426.643 Td /F1 9.8 Tf [(does not appear that simple motor deficits could explain these results. Although the z_Q175 mice responded more slowly than )] TJ ET BT 26.250 414.738 Td /F1 9.8 Tf [(WT controls at all times, they are still able to raise their response rate to ~40 RPM during the early part of the progressive ratio, )] TJ ET BT 26.250 402.834 Td /F1 9.8 Tf [(suggesting that their relatively slow responding in the FR5 period cannot be simply ascribed to an inability to respond faster; )] TJ ET BT 26.250 390.929 Td /F1 9.8 Tf [(more generally, as z_Q175 around 26 weeks of age show only mild hypoactivity )] TJ ET 0.267 0.267 0.267 rg BT 373.067 392.436 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 382.705 390.929 Td /F1 9.8 Tf [(, it is reasonable to assume that motor )] TJ ET BT 26.250 379.024 Td /F1 9.8 Tf [(deficits are not confounding this assessment of motivational state. Therefore, it is most likely that the observed deficits in )] TJ ET BT 26.250 367.119 Td /F1 9.8 Tf [(responding in these experiments do indeed reflect the pattern of motivation abnormalities outlined above, with only the aged )] TJ ET BT 26.250 355.215 Td /F1 9.8 Tf [(z_Q175 KI mice \(and their WT control subjects\) appearing to exhibit a motor deficit in terms of decreased maximum response )] TJ ET BT 26.250 343.310 Td /F1 9.8 Tf [(rate under both schedules of reinforcement, compared to all other mouse strains/ages.)] TJ ET BT 26.250 323.905 Td /F1 9.8 Tf [(Typically, progressive ratio studies in the literature report performance in terms of break points, recording the ratio requirement )] TJ ET BT 26.250 312.000 Td /F1 9.8 Tf [(at which the animals stop responding for a period of time as a measure of the maximum effort the subjects will impart )] TJ ET 0.267 0.267 0.267 rg BT 532.909 313.508 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 542.546 312.000 Td /F1 9.8 Tf [(. In the )] TJ ET BT 26.250 300.096 Td /F1 9.8 Tf [(present studies, likely as a consequence of the training procedure employed, the test animals failed to exhibit clear breakpoints )] TJ ET BT 26.250 288.191 Td /F1 9.8 Tf [(within the allowed intervals, continuing to make intermittent responses for long periods of time. Consequently, the approach )] TJ ET BT 26.250 276.286 Td /F1 9.8 Tf [(described here was implemented instead, measuring their work-rate per ratio requirement.)] TJ ET BT 26.250 256.881 Td /F1 9.8 Tf [(As has been described elsewhere )] TJ ET 0.267 0.267 0.267 rg BT 175.279 258.389 Td /F4 8.7 Tf [(22)] TJ ET 0.271 0.267 0.267 rg BT 184.916 256.881 Td /F1 9.8 Tf [(, operant tasks generally offer a very valuable tool for assessment of transgenic animals )] TJ ET BT 26.250 244.977 Td /F1 9.8 Tf [(generally and HD mouse models specifically, providing a reasonably high throughput, largely automated and sensitive set of )] TJ ET BT 26.250 233.072 Td /F1 9.8 Tf [(tools to assess these animals. This procedure, requiring only a lever press and a food magazine, is ideally suited to preclinical )] TJ ET BT 26.250 221.167 Td /F1 9.8 Tf [(screening in any facility with a simple operant setup and will ideally provide a helpful platform for preclinical screening to )] TJ ET BT 26.250 209.262 Td /F1 9.8 Tf [(ameliorate or prevent the emergence of motivation issues in HD patients.)] TJ ET BT 26.250 189.858 Td /F1 9.8 Tf [(In summary, the present data provide evidence for age- and genotype-dependent apathy/amotivational deficits in a mixed fixed-)] TJ ET BT 26.250 177.953 Td /F1 9.8 Tf [(/progressive-ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD full-length transgenic model )] TJ ET BT 26.250 166.048 Td /F1 9.8 Tf [(generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 145.463 167.555 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 155.101 166.048 Td /F1 9.8 Tf [( and the z_Q175 KI model )] TJ ET 0.267 0.267 0.267 rg BT 271.087 167.555 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 280.724 166.048 Td /F1 9.8 Tf [(. These data extend the range of HD characteristics currently )] TJ ET BT 26.250 154.143 Td /F1 9.8 Tf [(modeled in preclinical subjects, and may provide a valuable screening tool for novel HD therapeutics.)] TJ ET BT 26.250 117.541 Td /F4 12.0 Tf [(Competing Interest Statement)] TJ ET BT 26.250 97.587 Td /F1 9.8 Tf [(CHDI Foundation provides financial support for PLoS Currents: Huntington Disease. Editorial responsibility for all content )] TJ ET BT 26.250 85.682 Td /F1 9.8 Tf [(remains entirely within the remit of the Public Library of Science, the Editors, and Board of Reviewers.)] TJ ET BT 26.250 66.277 Td /F1 9.8 Tf [(Stephen Oakeshott, Russell Port, Judy Watson-Johnson, Jason Berger, Jane Sutphen, Neil E. Paterson, Sylvie Ramboz and )] TJ ET BT 26.250 54.372 Td /F1 9.8 Tf [(Dani Brunner are or were all employed by PsychoGenics, Inc., a for-profit institution.)] TJ ET Q q 15.000 44.491 577.500 732.509 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(These experiments indicate age- and genotype-dependent apathy or incentive motivation deficits in a mixed fixed/progressive-)] TJ ET BT 26.250 755.571 Td /F1 9.8 Tf [(ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD, a full-length transgenic model generated by )] TJ ET BT 26.250 743.667 Td /F1 9.8 Tf [(William Yang )] TJ ET 0.267 0.267 0.267 rg BT 85.842 745.174 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 95.479 743.667 Td /F1 9.8 Tf [(, and the z_Q175 KI model derived at Psychogenics )] TJ ET 0.267 0.267 0.267 rg BT 322.557 745.174 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 332.194 743.667 Td /F1 9.8 Tf [( from the CAG140 KI model originated in the laboratory )] TJ ET BT 26.250 731.762 Td /F1 9.8 Tf [(of Dr. Zeitlin )] TJ ET 0.267 0.267 0.267 rg BT 81.513 733.269 Td /F4 8.7 Tf [(18)] TJ ET 0.271 0.267 0.267 rg BT 91.150 731.762 Td /F1 9.8 Tf [(. Specifically, the BAC Tg mice exhibit deficits in the number of rewards obtained during PR training at both ages )] TJ ET BT 26.250 719.857 Td /F1 9.8 Tf [(tested compared to their WT controls. However, under the mixed FR/PR schedule, the older BAC Tg mice exhibited decreased )] TJ ET BT 26.250 707.952 Td /F1 9.8 Tf [(reinforcers earned, and decreased response rates at high ratio requirements in the PR component, with no observed )] TJ ET BT 26.250 696.048 Td /F1 9.8 Tf [(differences from WT controls in the FR component. By contrast, the younger BAC Tg mice exhibited only a trend \(p < 0.08\) )] TJ ET BT 26.250 684.143 Td /F1 9.8 Tf [(toward a significant decrease in response rate in the PR schedule. Thus, with increasing age, BAC HD mice exhibit the )] TJ ET BT 26.250 672.238 Td /F1 9.8 Tf [(emergence of a selective deficit in progressive-ratio versus fixed-ratio responding for a potent reinforcer \(evaporated milk\), )] TJ ET BT 26.250 660.333 Td /F1 9.8 Tf [(when data are expressed either as reinforcers earned or response rate at specific response ratios. By contrast, the younger )] TJ ET BT 26.250 648.429 Td /F1 9.8 Tf [(z_Q175 KI mice appear to reveal a more severe phenotype than do the BAC HD mice, with moderately decreased performance )] TJ ET BT 26.250 636.524 Td /F1 9.8 Tf [(at all phases of testing \(FR and PR schedules\), whether data were expressed as reinforcers earned or response rate per ratio, )] TJ ET BT 26.250 624.619 Td /F1 9.8 Tf [(compared to WT controls. Moreover, homozygous mice from this line were not able to acquire the basic lever pressing )] TJ ET BT 26.250 612.714 Td /F1 9.8 Tf [(response. In preliminary data from a small test cohort \(n=4-5\), older z_Q175 KI appeared to exhibit a less obvious phenotype. A )] TJ ET BT 26.250 600.810 Td /F1 9.8 Tf [(significant effect of genotype was observed only for the response rate in the FR component of the mixed FR/PR test procedure, )] TJ ET BT 26.250 588.905 Td /F1 9.8 Tf [(possibly due to the reduced level of responding in the WT controls \(i.e. a ceiling effect\). Based on this pattern of results, it may )] TJ ET BT 26.250 577.000 Td /F1 9.8 Tf [(be that the aged BAC Tg mice exhibit a selective deficit in motivation that is due to incentive motivation \(less goal directed )] TJ ET BT 26.250 565.095 Td /F1 9.8 Tf [(behavior or less wanting\) rather than hedonic motivation \(less pleasure derived from reward, or less liking\). Young z_Q175 )] TJ ET BT 26.250 553.191 Td /F1 9.8 Tf [(KI mice may exhibit a more profound deficit, involving both incentive and hedonic motivation, resulting in both FR and PR )] TJ ET BT 26.250 541.286 Td /F1 9.8 Tf [(deficits. Older z_Q175 KI and WT controls exhibited much lower levels of responding overall, reducing the opportunity to assess )] TJ ET BT 26.250 529.381 Td /F1 9.8 Tf [(genotype effects on motivation.)] TJ ET BT 26.250 509.976 Td /F1 9.8 Tf [(It is generally the case that these mice respond slightly more rapidly in the intermediate phase of the PR \(requiring ~30 )] TJ ET BT 26.250 498.072 Td /F1 9.8 Tf [(responses per reinforcer\) than during the FR5 periods, raising the possibility that these data could be influenced by some motor )] TJ ET BT 26.250 486.167 Td /F1 9.8 Tf [(issue preventing the HD mice from matching WT controls at these ratios. In Experiments 1A and 1B, it is clear from inspection )] TJ ET BT 26.250 474.262 Td /F1 9.8 Tf [(of these data that the underlying cause of the genotype differences in these experiments is related to a decline in response rate )] TJ ET BT 26.250 462.357 Td /F1 9.8 Tf [(in the HD animals as the ratios increase, rather than simply to a failure to match increased responding in the WTs. In )] TJ ET BT 26.250 450.453 Td /F1 9.8 Tf [(conjunction with the apparent ability of the BAC Tg mice to maintain their responding during the FR5 periods, it does not appear )] TJ ET BT 26.250 438.548 Td /F1 9.8 Tf [(that a simple motor phenotype could be responsible for these results. In the z_Q175 KI mice tested in Experiment 2B, it similarly )] TJ ET BT 26.250 426.643 Td /F1 9.8 Tf [(does not appear that simple motor deficits could explain these results. Although the z_Q175 mice responded more slowly than )] TJ ET BT 26.250 414.738 Td /F1 9.8 Tf [(WT controls at all times, they are still able to raise their response rate to ~40 RPM during the early part of the progressive ratio, )] TJ ET BT 26.250 402.834 Td /F1 9.8 Tf [(suggesting that their relatively slow responding in the FR5 period cannot be simply ascribed to an inability to respond faster; )] TJ ET BT 26.250 390.929 Td /F1 9.8 Tf [(more generally, as z_Q175 around 26 weeks of age show only mild hypoactivity )] TJ ET 0.267 0.267 0.267 rg BT 373.067 392.436 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 382.705 390.929 Td /F1 9.8 Tf [(, it is reasonable to assume that motor )] TJ ET BT 26.250 379.024 Td /F1 9.8 Tf [(deficits are not confounding this assessment of motivational state. Therefore, it is most likely that the observed deficits in )] TJ ET BT 26.250 367.119 Td /F1 9.8 Tf [(responding in these experiments do indeed reflect the pattern of motivation abnormalities outlined above, with only the aged )] TJ ET BT 26.250 355.215 Td /F1 9.8 Tf [(z_Q175 KI mice \(and their WT control subjects\) appearing to exhibit a motor deficit in terms of decreased maximum response )] TJ ET BT 26.250 343.310 Td /F1 9.8 Tf [(rate under both schedules of reinforcement, compared to all other mouse strains/ages.)] TJ ET BT 26.250 323.905 Td /F1 9.8 Tf [(Typically, progressive ratio studies in the literature report performance in terms of break points, recording the ratio requirement )] TJ ET BT 26.250 312.000 Td /F1 9.8 Tf [(at which the animals stop responding for a period of time as a measure of the maximum effort the subjects will impart )] TJ ET 0.267 0.267 0.267 rg BT 532.909 313.508 Td /F4 8.7 Tf [(13)] TJ ET 0.271 0.267 0.267 rg BT 542.546 312.000 Td /F1 9.8 Tf [(. In the )] TJ ET BT 26.250 300.096 Td /F1 9.8 Tf [(present studies, likely as a consequence of the training procedure employed, the test animals failed to exhibit clear breakpoints )] TJ ET BT 26.250 288.191 Td /F1 9.8 Tf [(within the allowed intervals, continuing to make intermittent responses for long periods of time. Consequently, the approach )] TJ ET BT 26.250 276.286 Td /F1 9.8 Tf [(described here was implemented instead, measuring their work-rate per ratio requirement.)] TJ ET BT 26.250 256.881 Td /F1 9.8 Tf [(As has been described elsewhere )] TJ ET 0.267 0.267 0.267 rg BT 175.279 258.389 Td /F4 8.7 Tf [(22)] TJ ET 0.271 0.267 0.267 rg BT 184.916 256.881 Td /F1 9.8 Tf [(, operant tasks generally offer a very valuable tool for assessment of transgenic animals )] TJ ET BT 26.250 244.977 Td /F1 9.8 Tf [(generally and HD mouse models specifically, providing a reasonably high throughput, largely automated and sensitive set of )] TJ ET BT 26.250 233.072 Td /F1 9.8 Tf [(tools to assess these animals. This procedure, requiring only a lever press and a food magazine, is ideally suited to preclinical )] TJ ET BT 26.250 221.167 Td /F1 9.8 Tf [(screening in any facility with a simple operant setup and will ideally provide a helpful platform for preclinical screening to )] TJ ET BT 26.250 209.262 Td /F1 9.8 Tf [(ameliorate or prevent the emergence of motivation issues in HD patients.)] TJ ET BT 26.250 189.858 Td /F1 9.8 Tf [(In summary, the present data provide evidence for age- and genotype-dependent apathy/amotivational deficits in a mixed fixed-)] TJ ET BT 26.250 177.953 Td /F1 9.8 Tf [(/progressive-ratio \(FR/PR\) schedule of reinforcement in two mouse models of HD, the BAC HD full-length transgenic model )] TJ ET BT 26.250 166.048 Td /F1 9.8 Tf [(generated by William Yang )] TJ ET 0.267 0.267 0.267 rg BT 145.463 167.555 Td /F4 8.7 Tf [(15)] TJ ET 0.271 0.267 0.267 rg BT 155.101 166.048 Td /F1 9.8 Tf [( and the z_Q175 KI model )] TJ ET 0.267 0.267 0.267 rg BT 271.087 167.555 Td /F4 8.7 Tf [(19)] TJ ET 0.271 0.267 0.267 rg BT 280.724 166.048 Td /F1 9.8 Tf [(. These data extend the range of HD characteristics currently )] TJ ET BT 26.250 154.143 Td /F1 9.8 Tf [(modeled in preclinical subjects, and may provide a valuable screening tool for novel HD therapeutics.)] TJ ET BT 26.250 117.541 Td /F4 12.0 Tf [(Competing Interest Statement)] TJ ET BT 26.250 97.587 Td /F1 9.8 Tf [(CHDI Foundation provides financial support for PLoS Currents: Huntington Disease. Editorial responsibility for all content )] TJ ET BT 26.250 85.682 Td /F1 9.8 Tf [(remains entirely within the remit of the Public Library of Science, the Editors, and Board of Reviewers.)] TJ ET BT 26.250 66.277 Td /F1 9.8 Tf [(Stephen Oakeshott, Russell Port, Judy Watson-Johnson, Jason Berger, Jane Sutphen, Neil E. Paterson, Sylvie Ramboz and )] TJ ET BT 26.250 54.372 Td /F1 9.8 Tf [(Dani Brunner are or were all employed by PsychoGenics, Inc., a for-profit institution.)] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(9)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 367 0 obj << /Type /Annot /Subtype /Link /A 368 0 R /Border [0 0 0] /H /I /Rect [ 85.8420 744.3722 95.4793 753.1905 ] >> endobj 368 0 obj << /Type /Action >> endobj 369 0 obj << /Type /Annot /Subtype /Link /A 370 0 R /Border [0 0 0] /H /I /Rect [ 322.5568 744.3722 332.1942 753.1905 ] >> endobj 370 0 obj << /Type /Action >> endobj 371 0 obj << /Type /Annot /Subtype /Link /A 372 0 R /Border [0 0 0] /H /I /Rect [ 81.5130 732.4674 91.1503 741.2858 ] >> endobj 372 0 obj << /Type /Action >> endobj 373 0 obj << /Type /Annot /Subtype /Link /A 374 0 R /Border [0 0 0] /H /I /Rect [ 373.0673 391.6344 382.7046 400.4528 ] >> endobj 374 0 obj << /Type /Action >> endobj 375 0 obj << /Type /Annot /Subtype /Link /A 376 0 R /Border [0 0 0] /H /I /Rect [ 532.9088 312.7059 542.5461 321.5243 ] >> endobj 376 0 obj << /Type /Action >> endobj 377 0 obj << /Type /Annot /Subtype /Link /A 378 0 R /Border [0 0 0] /H /I /Rect [ 175.2788 257.5869 184.9161 266.4053 ] >> endobj 378 0 obj << /Type /Action >> endobj 379 0 obj << /Type /Annot /Subtype /Link /A 380 0 R /Border [0 0 0] /H /I /Rect [ 145.4633 166.7537 155.1006 175.5720 ] >> endobj 380 0 obj << /Type /Action >> endobj 381 0 obj << /Type /Annot /Subtype /Link /A 382 0 R /Border [0 0 0] /H /I /Rect [ 271.0866 166.7537 280.7239 175.5720 ] >> endobj 382 0 obj << /Type /Action >> endobj 383 0 obj << /Type /Annot /Subtype /Link /A 384 0 R /Border [0 0 0] /H /I /Rect [ 85.8420 744.3722 95.4793 753.1905 ] >> endobj 384 0 obj << /Type /Action >> endobj 385 0 obj << /Type /Annot /Subtype /Link /A 386 0 R /Border [0 0 0] /H /I /Rect [ 322.5568 744.3722 332.1942 753.1905 ] >> endobj 386 0 obj << /Type /Action >> endobj 387 0 obj << /Type /Annot /Subtype /Link /A 388 0 R /Border [0 0 0] /H /I /Rect [ 81.5130 732.4674 91.1503 741.2858 ] >> endobj 388 0 obj << /Type /Action >> endobj 389 0 obj << /Type /Annot /Subtype /Link /A 390 0 R /Border [0 0 0] /H /I /Rect [ 373.0673 391.6344 382.7046 400.4528 ] >> endobj 390 0 obj << /Type /Action >> endobj 391 0 obj << /Type /Annot /Subtype /Link /A 392 0 R /Border [0 0 0] /H /I /Rect [ 532.9088 312.7059 542.5461 321.5243 ] >> endobj 392 0 obj << /Type /Action >> endobj 393 0 obj << /Type /Annot /Subtype /Link /A 394 0 R /Border [0 0 0] /H /I /Rect [ 175.2788 257.5869 184.9161 266.4053 ] >> endobj 394 0 obj << /Type /Action >> endobj 395 0 obj << /Type /Annot /Subtype /Link /A 396 0 R /Border [0 0 0] /H /I /Rect [ 145.4633 166.7537 155.1006 175.5720 ] >> endobj 396 0 obj << /Type /Action >> endobj 397 0 obj << /Type /Annot /Subtype /Link /A 398 0 R /Border [0 0 0] /H /I /Rect [ 271.0866 166.7537 280.7239 175.5720 ] >> endobj 398 0 obj << /Type /Action >> endobj 399 0 obj << /Type /Annot /Subtype /Link /A 400 0 R /Border [0 0 0] /H /I /Rect [ 85.8420 744.3722 95.4793 753.1905 ] >> endobj 400 0 obj << /Type /Action >> endobj 401 0 obj << /Type /Annot /Subtype /Link /A 402 0 R /Border [0 0 0] /H /I /Rect [ 322.5568 744.3722 332.1942 753.1905 ] >> endobj 402 0 obj << /Type /Action >> endobj 403 0 obj << /Type /Annot /Subtype /Link /A 404 0 R /Border [0 0 0] /H /I /Rect [ 81.5130 732.4674 91.1503 741.2858 ] >> endobj 404 0 obj << /Type /Action >> endobj 405 0 obj << /Type /Annot /Subtype /Link /A 406 0 R /Border [0 0 0] /H /I /Rect [ 373.0673 391.6344 382.7046 400.4528 ] >> endobj 406 0 obj << /Type /Action >> endobj 407 0 obj << /Type /Annot /Subtype /Link /A 408 0 R /Border [0 0 0] /H /I /Rect [ 532.9088 312.7059 542.5461 321.5243 ] >> endobj 408 0 obj << /Type /Action >> endobj 409 0 obj << /Type /Annot /Subtype /Link /A 410 0 R /Border [0 0 0] /H /I /Rect [ 175.2788 257.5869 184.9161 266.4053 ] >> endobj 410 0 obj << /Type /Action >> endobj 411 0 obj << /Type /Annot /Subtype /Link /A 412 0 R /Border [0 0 0] /H /I /Rect [ 145.4633 166.7537 155.1006 175.5720 ] >> endobj 412 0 obj << /Type /Action >> endobj 413 0 obj << /Type /Annot /Subtype /Link /A 414 0 R /Border [0 0 0] /H /I /Rect [ 271.0866 166.7537 280.7239 175.5720 ] >> endobj 414 0 obj << /Type /Action >> endobj 415 0 obj << /Type /Page /Parent 3 0 R /Annots [ 417 0 R 419 0 R 421 0 R 423 0 R 425 0 R 427 0 R 429 0 R 431 0 R 433 0 R 435 0 R 437 0 R 439 0 R 441 0 R 443 0 R 445 0 R 447 0 R 449 0 R 451 0 R 453 0 R 455 0 R 457 0 R 459 0 R 461 0 R 463 0 R 465 0 R 467 0 R 469 0 R 471 0 R 473 0 R 475 0 R 477 0 R 479 0 R 481 0 R 483 0 R 485 0 R 487 0 R 489 0 R 491 0 R 493 0 R 495 0 R 497 0 R 499 0 R 501 0 R 503 0 R 505 0 R 507 0 R 509 0 R 511 0 R ] /Contents 416 0 R >> endobj 416 0 obj << /Length 21314 >> stream 0.271 0.267 0.267 rg q 15.000 40.834 577.500 736.166 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(The authors have declared that no further competing interests exist.)] TJ ET BT 26.250 730.874 Td /F4 12.0 Tf [(Acknowledgements)] TJ ET BT 26.250 710.919 Td /F1 9.8 Tf [(Thanks to Simon Brooks, Stephen Dunnett, Liliana Menalled, Carol Murphy, and Fuat Balci for helpful discussions and to )] TJ ET BT 26.250 699.015 Td /F1 9.8 Tf [(Melinda Ruiz, David Connor and Diana Carlsen for technical support. BAC HD mice were provided courtesy of X. William Yang )] TJ ET BT 26.250 687.110 Td /F1 9.8 Tf [(\(UCLA David Geffen School of Medicine, Los Angeles CA\).)] TJ ET BT 26.250 658.007 Td /F4 12.0 Tf [(References)] TJ ET BT 26.250 630.553 Td /F1 9.8 Tf [(1.)] TJ ET BT 38.132 630.553 Td /F1 9.8 Tf [(Roos RA, Hermans J, Vegter-van der Vlis M, van Ommen GJ, Bruyn GW \(1993\) Duration of illness in Huntington's disease is )] TJ ET BT 26.250 618.648 Td /F1 9.8 Tf [(not related to age at onset. J Neurol Neurosurg Psychiatry 56: 98-100.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.163 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 591.612 Td /F1 9.8 Tf [(2.)] TJ ET BT 38.132 591.612 Td /F1 9.8 Tf [(Craufurd D, Snowden J \(2002\) Neuropsychological and neuropsychiatric aspects of Huntingtons Disease In: Bates G, )] TJ ET BT 26.250 579.708 Td /F1 9.8 Tf [(Harper PS, Jones L, editors. Huntington's Disease. Oxford: Oxford University Press. pp. 62-94.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.222 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 552.672 Td /F1 9.8 Tf [(3.)] TJ ET BT 38.132 552.672 Td /F1 9.8 Tf [( Ishizaki J, Mimura M \(2011\) Dysthymia and apathy: diagnosis and treatment. Depress Res Treat 2011: 893905.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 544.186 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 525.636 Td /F1 9.8 Tf [(4.)] TJ ET BT 38.132 525.636 Td /F1 9.8 Tf [(Caine ED, Shoulson I \(1983\) Psychiatric syndromes in Huntington's disease. Am J Psychiatry 140: 728-733.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 517.150 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 498.600 Td /F1 9.8 Tf [(5.)] TJ ET BT 38.132 498.600 Td /F1 9.8 Tf [(Marin RS, Biedrzycki RC, Firinciogullari S \(1991\) Reliability and validity of the apathy evaluation scale. Psychiatry Research )] TJ ET BT 26.250 486.695 Td /F1 9.8 Tf [(38: 143-162.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 478.209 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 459.659 Td /F1 9.8 Tf [(6.)] TJ ET BT 38.132 459.659 Td /F1 9.8 Tf [(Marin RS \(1991\) Apathy: a neuropsychiatric syndrome. J Neuropsychiatry Clin Neurosci 3: 243-254.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 451.173 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 432.623 Td /F1 9.8 Tf [(7.)] TJ ET BT 38.132 432.623 Td /F1 9.8 Tf [(Clarke DE, Ko JY, Kuhl EA, van Reekum R, Salvador R, et al. \(2011\) Are the available apathy measures reliable and valid? )] TJ ET BT 26.250 420.718 Td /F1 9.8 Tf [(A review of the psychometric evidence. J Psychosom Res 70: 73-97.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 412.232 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 393.682 Td /F1 9.8 Tf [(8.)] TJ ET BT 38.132 393.682 Td /F1 9.8 Tf [(Barbano MF, Cador M \(2007\) Opioids for hedonic experience and dopamine to get ready for it. Psychopharmacology \(Berl\) )] TJ ET BT 26.250 381.777 Td /F1 9.8 Tf [(191: 497-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 373.291 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 354.741 Td /F1 9.8 Tf [(9.)] TJ ET BT 38.132 354.741 Td /F1 9.8 Tf [(Gard DE, Kring AM, Gard MG, Horan WP, Green MF \(2007\) Anhedonia in schizophrenia: distinctions between anticipatory )] TJ ET BT 26.250 342.837 Td /F1 9.8 Tf [(and consummatory pleasure. Schizophr Res 93: 253-260.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 334.351 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 315.801 Td /F1 9.8 Tf [(10.)] TJ ET BT 43.553 315.801 Td /F1 9.8 Tf [(Treadway MT, Zald DH \(2011\) Reconsidering anhedonia in depression: lessons from translational neuroscience. Neurosci )] TJ ET BT 26.250 303.896 Td /F1 9.8 Tf [(Biobehav Rev 35: 537-555.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 295.410 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 276.860 Td /F1 9.8 Tf [(11.)] TJ ET BT 43.553 276.860 Td /F1 9.8 Tf [( Bindra D \(1978\) How adaptive behavior is produced: a perceptual-motivational alternative to response reinforcements. )] TJ ET BT 26.250 264.955 Td /F1 9.8 Tf [(Behavioral and Brain Sciences 1: 41-52.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 256.469 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 237.919 Td /F1 9.8 Tf [(12.)] TJ ET BT 43.553 237.919 Td /F1 9.8 Tf [(Arnold JM, Roberts DC \(1997\) A critique of fixed and progressive ratio schedules used to examine the neural substrates of )] TJ ET BT 26.250 226.014 Td /F1 9.8 Tf [(drug reinforcement. Pharmacol Biochem Behav 57: 441-447.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 217.528 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 198.978 Td /F1 9.8 Tf [(13.)] TJ ET BT 43.553 198.978 Td /F1 9.8 Tf [(Hodos W \(1961\) Progressive ratio as a measure of reward strength. Science 134: 943-944.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 190.492 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 171.942 Td /F1 9.8 Tf [(14.)] TJ ET BT 43.553 171.942 Td /F1 9.8 Tf [(Killeen PR, Posadas-Sanchez D, Johansen EB, Thrailkill EA \(2009\) Progressive ratio schedules of reinforcement. J Exp )] TJ ET BT 26.250 160.038 Td /F1 9.8 Tf [(Psychol Anim Behav Process 35: 35-50.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 151.552 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 133.002 Td /F1 9.8 Tf [(15.)] TJ ET BT 43.553 133.002 Td /F1 9.8 Tf [(Gray M, Shirasaki DI, Cepeda C, Andre VM, Wilburn B, et al. \(2008\) Full-length human mutant huntingtin with a stable )] TJ ET BT 26.250 121.097 Td /F1 9.8 Tf [(polyglutamine repeat can elicit progressive and selective neuropathogenesis in BACHD mice. J Neurosci 28: 6182-6195.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 112.611 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 94.061 Td /F1 9.8 Tf [(16.)] TJ ET BT 43.553 94.061 Td /F1 9.8 Tf [(Hodgson JG, Agopyan N, Gutekunst CA, Leavitt BR, LePiane F, et al. \(1999\) A YAC mouse model for Huntington's disease )] TJ ET BT 26.250 82.156 Td /F1 9.8 Tf [(with full-length mutant huntingtin, cytoplasmic toxicity, and selective striatal neurodegeneration. Neuron 23: 181-192.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 73.670 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 55.120 Td /F1 9.8 Tf [(17.)] TJ ET BT 43.553 55.120 Td /F1 9.8 Tf [(Mangiarini L, Sathasivam K, Seller M, Cozens B, Harper A, et al. \(1996\) Exon 1 of the HD gene with an expanded CAG )] TJ ET Q q 15.000 40.834 577.500 736.166 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(The authors have declared that no further competing interests exist.)] TJ ET BT 26.250 730.874 Td /F4 12.0 Tf [(Acknowledgements)] TJ ET BT 26.250 710.919 Td /F1 9.8 Tf [(Thanks to Simon Brooks, Stephen Dunnett, Liliana Menalled, Carol Murphy, and Fuat Balci for helpful discussions and to )] TJ ET BT 26.250 699.015 Td /F1 9.8 Tf [(Melinda Ruiz, David Connor and Diana Carlsen for technical support. BAC HD mice were provided courtesy of X. William Yang )] TJ ET BT 26.250 687.110 Td /F1 9.8 Tf [(\(UCLA David Geffen School of Medicine, Los Angeles CA\).)] TJ ET BT 26.250 658.007 Td /F4 12.0 Tf [(References)] TJ ET BT 26.250 630.553 Td /F1 9.8 Tf [(1.)] TJ ET BT 38.132 630.553 Td /F1 9.8 Tf [(Roos RA, Hermans J, Vegter-van der Vlis M, van Ommen GJ, Bruyn GW \(1993\) Duration of illness in Huntington's disease is )] TJ ET BT 26.250 618.648 Td /F1 9.8 Tf [(not related to age at onset. J Neurol Neurosurg Psychiatry 56: 98-100.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.163 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 591.612 Td /F1 9.8 Tf [(2.)] TJ ET BT 38.132 591.612 Td /F1 9.8 Tf [(Craufurd D, Snowden J \(2002\) Neuropsychological and neuropsychiatric aspects of Huntingtons Disease In: Bates G, )] TJ ET BT 26.250 579.708 Td /F1 9.8 Tf [(Harper PS, Jones L, editors. Huntington's Disease. Oxford: Oxford University Press. pp. 62-94.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.222 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 552.672 Td /F1 9.8 Tf [(3.)] TJ ET BT 38.132 552.672 Td /F1 9.8 Tf [( Ishizaki J, Mimura M \(2011\) Dysthymia and apathy: diagnosis and treatment. Depress Res Treat 2011: 893905.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 544.186 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 525.636 Td /F1 9.8 Tf [(4.)] TJ ET BT 38.132 525.636 Td /F1 9.8 Tf [(Caine ED, Shoulson I \(1983\) Psychiatric syndromes in Huntington's disease. Am J Psychiatry 140: 728-733.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 517.150 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 498.600 Td /F1 9.8 Tf [(5.)] TJ ET BT 38.132 498.600 Td /F1 9.8 Tf [(Marin RS, Biedrzycki RC, Firinciogullari S \(1991\) Reliability and validity of the apathy evaluation scale. Psychiatry Research )] TJ ET BT 26.250 486.695 Td /F1 9.8 Tf [(38: 143-162.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 478.209 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 459.659 Td /F1 9.8 Tf [(6.)] TJ ET BT 38.132 459.659 Td /F1 9.8 Tf [(Marin RS \(1991\) Apathy: a neuropsychiatric syndrome. J Neuropsychiatry Clin Neurosci 3: 243-254.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 451.173 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 432.623 Td /F1 9.8 Tf [(7.)] TJ ET BT 38.132 432.623 Td /F1 9.8 Tf [(Clarke DE, Ko JY, Kuhl EA, van Reekum R, Salvador R, et al. \(2011\) Are the available apathy measures reliable and valid? )] TJ ET BT 26.250 420.718 Td /F1 9.8 Tf [(A review of the psychometric evidence. J Psychosom Res 70: 73-97.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 412.232 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 393.682 Td /F1 9.8 Tf [(8.)] TJ ET BT 38.132 393.682 Td /F1 9.8 Tf [(Barbano MF, Cador M \(2007\) Opioids for hedonic experience and dopamine to get ready for it. Psychopharmacology \(Berl\) )] TJ ET BT 26.250 381.777 Td /F1 9.8 Tf [(191: 497-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 373.291 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 354.741 Td /F1 9.8 Tf [(9.)] TJ ET BT 38.132 354.741 Td /F1 9.8 Tf [(Gard DE, Kring AM, Gard MG, Horan WP, Green MF \(2007\) Anhedonia in schizophrenia: distinctions between anticipatory )] TJ ET BT 26.250 342.837 Td /F1 9.8 Tf [(and consummatory pleasure. Schizophr Res 93: 253-260.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 334.351 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 315.801 Td /F1 9.8 Tf [(10.)] TJ ET BT 43.553 315.801 Td /F1 9.8 Tf [(Treadway MT, Zald DH \(2011\) Reconsidering anhedonia in depression: lessons from translational neuroscience. Neurosci )] TJ ET BT 26.250 303.896 Td /F1 9.8 Tf [(Biobehav Rev 35: 537-555.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 295.410 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 276.860 Td /F1 9.8 Tf [(11.)] TJ ET BT 43.553 276.860 Td /F1 9.8 Tf [( Bindra D \(1978\) How adaptive behavior is produced: a perceptual-motivational alternative to response reinforcements. )] TJ ET BT 26.250 264.955 Td /F1 9.8 Tf [(Behavioral and Brain Sciences 1: 41-52.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 256.469 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 237.919 Td /F1 9.8 Tf [(12.)] TJ ET BT 43.553 237.919 Td /F1 9.8 Tf [(Arnold JM, Roberts DC \(1997\) A critique of fixed and progressive ratio schedules used to examine the neural substrates of )] TJ ET BT 26.250 226.014 Td /F1 9.8 Tf [(drug reinforcement. Pharmacol Biochem Behav 57: 441-447.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 217.528 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 198.978 Td /F1 9.8 Tf [(13.)] TJ ET BT 43.553 198.978 Td /F1 9.8 Tf [(Hodos W \(1961\) Progressive ratio as a measure of reward strength. Science 134: 943-944.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 190.492 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 171.942 Td /F1 9.8 Tf [(14.)] TJ ET BT 43.553 171.942 Td /F1 9.8 Tf [(Killeen PR, Posadas-Sanchez D, Johansen EB, Thrailkill EA \(2009\) Progressive ratio schedules of reinforcement. J Exp )] TJ ET BT 26.250 160.038 Td /F1 9.8 Tf [(Psychol Anim Behav Process 35: 35-50.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 151.552 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 133.002 Td /F1 9.8 Tf [(15.)] TJ ET BT 43.553 133.002 Td /F1 9.8 Tf [(Gray M, Shirasaki DI, Cepeda C, Andre VM, Wilburn B, et al. \(2008\) Full-length human mutant huntingtin with a stable )] TJ ET BT 26.250 121.097 Td /F1 9.8 Tf [(polyglutamine repeat can elicit progressive and selective neuropathogenesis in BACHD mice. J Neurosci 28: 6182-6195.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 112.611 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 94.061 Td /F1 9.8 Tf [(16.)] TJ ET BT 43.553 94.061 Td /F1 9.8 Tf [(Hodgson JG, Agopyan N, Gutekunst CA, Leavitt BR, LePiane F, et al. \(1999\) A YAC mouse model for Huntington's disease )] TJ ET BT 26.250 82.156 Td /F1 9.8 Tf [(with full-length mutant huntingtin, cytoplasmic toxicity, and selective striatal neurodegeneration. Neuron 23: 181-192.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 73.670 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 55.120 Td /F1 9.8 Tf [(17.)] TJ ET BT 43.553 55.120 Td /F1 9.8 Tf [(Mangiarini L, Sathasivam K, Seller M, Cozens B, Harper A, et al. \(1996\) Exon 1 of the HD gene with an expanded CAG )] TJ ET Q q 15.000 40.834 577.500 736.166 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(The authors have declared that no further competing interests exist.)] TJ ET BT 26.250 730.874 Td /F4 12.0 Tf [(Acknowledgements)] TJ ET BT 26.250 710.919 Td /F1 9.8 Tf [(Thanks to Simon Brooks, Stephen Dunnett, Liliana Menalled, Carol Murphy, and Fuat Balci for helpful discussions and to )] TJ ET BT 26.250 699.015 Td /F1 9.8 Tf [(Melinda Ruiz, David Connor and Diana Carlsen for technical support. BAC HD mice were provided courtesy of X. William Yang )] TJ ET BT 26.250 687.110 Td /F1 9.8 Tf [(\(UCLA David Geffen School of Medicine, Los Angeles CA\).)] TJ ET BT 26.250 658.007 Td /F4 12.0 Tf [(References)] TJ ET BT 26.250 630.553 Td /F1 9.8 Tf [(1.)] TJ ET BT 38.132 630.553 Td /F1 9.8 Tf [(Roos RA, Hermans J, Vegter-van der Vlis M, van Ommen GJ, Bruyn GW \(1993\) Duration of illness in Huntington's disease is )] TJ ET BT 26.250 618.648 Td /F1 9.8 Tf [(not related to age at onset. J Neurol Neurosurg Psychiatry 56: 98-100.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.163 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 591.612 Td /F1 9.8 Tf [(2.)] TJ ET BT 38.132 591.612 Td /F1 9.8 Tf [(Craufurd D, Snowden J \(2002\) Neuropsychological and neuropsychiatric aspects of Huntingtons Disease In: Bates G, )] TJ ET BT 26.250 579.708 Td /F1 9.8 Tf [(Harper PS, Jones L, editors. Huntington's Disease. Oxford: Oxford University Press. pp. 62-94.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.222 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 552.672 Td /F1 9.8 Tf [(3.)] TJ ET BT 38.132 552.672 Td /F1 9.8 Tf [( Ishizaki J, Mimura M \(2011\) Dysthymia and apathy: diagnosis and treatment. Depress Res Treat 2011: 893905.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 544.186 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 525.636 Td /F1 9.8 Tf [(4.)] TJ ET BT 38.132 525.636 Td /F1 9.8 Tf [(Caine ED, Shoulson I \(1983\) Psychiatric syndromes in Huntington's disease. Am J Psychiatry 140: 728-733.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 517.150 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 498.600 Td /F1 9.8 Tf [(5.)] TJ ET BT 38.132 498.600 Td /F1 9.8 Tf [(Marin RS, Biedrzycki RC, Firinciogullari S \(1991\) Reliability and validity of the apathy evaluation scale. Psychiatry Research )] TJ ET BT 26.250 486.695 Td /F1 9.8 Tf [(38: 143-162.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 478.209 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 459.659 Td /F1 9.8 Tf [(6.)] TJ ET BT 38.132 459.659 Td /F1 9.8 Tf [(Marin RS \(1991\) Apathy: a neuropsychiatric syndrome. J Neuropsychiatry Clin Neurosci 3: 243-254.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 451.173 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 432.623 Td /F1 9.8 Tf [(7.)] TJ ET BT 38.132 432.623 Td /F1 9.8 Tf [(Clarke DE, Ko JY, Kuhl EA, van Reekum R, Salvador R, et al. \(2011\) Are the available apathy measures reliable and valid? )] TJ ET BT 26.250 420.718 Td /F1 9.8 Tf [(A review of the psychometric evidence. J Psychosom Res 70: 73-97.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 412.232 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 393.682 Td /F1 9.8 Tf [(8.)] TJ ET BT 38.132 393.682 Td /F1 9.8 Tf [(Barbano MF, Cador M \(2007\) Opioids for hedonic experience and dopamine to get ready for it. Psychopharmacology \(Berl\) )] TJ ET BT 26.250 381.777 Td /F1 9.8 Tf [(191: 497-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 373.291 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 354.741 Td /F1 9.8 Tf [(9.)] TJ ET BT 38.132 354.741 Td /F1 9.8 Tf [(Gard DE, Kring AM, Gard MG, Horan WP, Green MF \(2007\) Anhedonia in schizophrenia: distinctions between anticipatory )] TJ ET BT 26.250 342.837 Td /F1 9.8 Tf [(and consummatory pleasure. Schizophr Res 93: 253-260.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 334.351 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 315.801 Td /F1 9.8 Tf [(10.)] TJ ET BT 43.553 315.801 Td /F1 9.8 Tf [(Treadway MT, Zald DH \(2011\) Reconsidering anhedonia in depression: lessons from translational neuroscience. Neurosci )] TJ ET BT 26.250 303.896 Td /F1 9.8 Tf [(Biobehav Rev 35: 537-555.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 295.410 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 276.860 Td /F1 9.8 Tf [(11.)] TJ ET BT 43.553 276.860 Td /F1 9.8 Tf [( Bindra D \(1978\) How adaptive behavior is produced: a perceptual-motivational alternative to response reinforcements. )] TJ ET BT 26.250 264.955 Td /F1 9.8 Tf [(Behavioral and Brain Sciences 1: 41-52.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 256.469 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 237.919 Td /F1 9.8 Tf [(12.)] TJ ET BT 43.553 237.919 Td /F1 9.8 Tf [(Arnold JM, Roberts DC \(1997\) A critique of fixed and progressive ratio schedules used to examine the neural substrates of )] TJ ET BT 26.250 226.014 Td /F1 9.8 Tf [(drug reinforcement. Pharmacol Biochem Behav 57: 441-447.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 217.528 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 198.978 Td /F1 9.8 Tf [(13.)] TJ ET BT 43.553 198.978 Td /F1 9.8 Tf [(Hodos W \(1961\) Progressive ratio as a measure of reward strength. Science 134: 943-944.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 190.492 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 171.942 Td /F1 9.8 Tf [(14.)] TJ ET BT 43.553 171.942 Td /F1 9.8 Tf [(Killeen PR, Posadas-Sanchez D, Johansen EB, Thrailkill EA \(2009\) Progressive ratio schedules of reinforcement. J Exp )] TJ ET BT 26.250 160.038 Td /F1 9.8 Tf [(Psychol Anim Behav Process 35: 35-50.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 151.552 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 133.002 Td /F1 9.8 Tf [(15.)] TJ ET BT 43.553 133.002 Td /F1 9.8 Tf [(Gray M, Shirasaki DI, Cepeda C, Andre VM, Wilburn B, et al. \(2008\) Full-length human mutant huntingtin with a stable )] TJ ET BT 26.250 121.097 Td /F1 9.8 Tf [(polyglutamine repeat can elicit progressive and selective neuropathogenesis in BACHD mice. J Neurosci 28: 6182-6195.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 112.611 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 94.061 Td /F1 9.8 Tf [(16.)] TJ ET BT 43.553 94.061 Td /F1 9.8 Tf [(Hodgson JG, Agopyan N, Gutekunst CA, Leavitt BR, LePiane F, et al. \(1999\) A YAC mouse model for Huntington's disease )] TJ ET BT 26.250 82.156 Td /F1 9.8 Tf [(with full-length mutant huntingtin, cytoplasmic toxicity, and selective striatal neurodegeneration. Neuron 23: 181-192.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 73.670 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 55.120 Td /F1 9.8 Tf [(17.)] TJ ET BT 43.553 55.120 Td /F1 9.8 Tf [(Mangiarini L, Sathasivam K, Seller M, Cozens B, Harper A, et al. \(1996\) Exon 1 of the HD gene with an expanded CAG )] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(10)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 417 0 obj << /Type /Annot /Subtype /Link /A 418 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 609.4688 91.2600 617.1000 ] >> endobj 418 0 obj << /Type /Action /S /URI /URI (https://jnnp.bmj.com/content/56/1/98.full.pdf+html) >> endobj 419 0 obj << /Type /Annot /Subtype /Link /A 420 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 570.5280 91.2600 578.1592 ] >> endobj 420 0 obj << /Type /Action /S /URI /URI (https://ukcatalogue.oup.com/product/9780198510604.do) >> endobj 421 0 obj << /Type /Annot /Subtype /Link /A 422 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 543.4920 91.2600 551.1232 ] >> endobj 422 0 obj << /Type /Action /S /URI /URI (https://www.hindawi.com/journals/drt/2011/893905/) >> endobj 423 0 obj << /Type /Annot /Subtype /Link /A 424 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 516.4560 91.2600 524.0873 ] >> endobj 424 0 obj << /Type /Action /S /URI /URI (https://ajp.psychiatryonline.org/article.aspx?volume=140&page=728) >> endobj 425 0 obj << /Type /Annot /Subtype /Link /A 426 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 477.5152 91.2600 485.1465 ] >> endobj 426 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/016517819190040V) >> endobj 427 0 obj << /Type /Annot /Subtype /Link /A 428 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 450.4792 91.2600 458.1105 ] >> endobj 428 0 obj << /Type /Action /S /URI /URI (https://neuro.psychiatryonline.org/article.aspx?volume=3&page=243) >> endobj 429 0 obj << /Type /Annot /Subtype /Link /A 430 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 411.5385 91.2600 419.1697 ] >> endobj 430 0 obj << /Type /Action /S /URI /URI (https://www.jpsychores.com/article/S0022-3999\(10\)00017-6/abstract) >> endobj 431 0 obj << /Type /Annot /Subtype /Link /A 432 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 372.5977 91.2600 380.2290 ] >> endobj 432 0 obj << /Type /Action /S /URI /URI (https://www.springerlink.com/content/dwm51828866p8522/) >> endobj 433 0 obj << /Type /Annot /Subtype /Link /A 434 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 333.6570 91.2600 341.2882 ] >> endobj 434 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1986826/) >> endobj 435 0 obj << /Type /Annot /Subtype /Link /A 436 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 294.7162 91.2600 302.3475 ] >> endobj 436 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005986/?tool=pubmed) >> endobj 437 0 obj << /Type /Annot /Subtype /Link /A 438 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 255.7755 91.2600 263.4067 ] >> endobj 438 0 obj << /Type /Action /S /URI /URI (https://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=7133780) >> endobj 439 0 obj << /Type /Annot /Subtype /Link /A 440 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 216.8347 91.2600 224.4660 ] >> endobj 440 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0091305796004455) >> endobj 441 0 obj << /Type /Annot /Subtype /Link /A 442 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 189.7987 91.2600 197.4300 ] >> endobj 442 0 obj << /Type /Action /S /URI /URI (https://www.sciencemag.org/content/134/3483/943.short) >> endobj 443 0 obj << /Type /Annot /Subtype /Link /A 444 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 150.8580 91.2600 158.4892 ] >> endobj 444 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806234/?tool=pubmed) >> endobj 445 0 obj << /Type /Annot /Subtype /Link /A 446 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 111.9172 91.2600 119.5485 ] >> endobj 446 0 obj << /Type /Action /S /URI /URI (https://www.jneurosci.org/content/28/24/6182.short) >> endobj 447 0 obj << /Type /Annot /Subtype /Link /A 448 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 72.9765 91.2600 80.6077 ] >> endobj 448 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/neuron/retrieve/pii/S0896627300807643) >> endobj 449 0 obj << /Type /Annot /Subtype /Link /A 450 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 609.4688 91.2600 617.1000 ] >> endobj 450 0 obj << /Type /Action /S /URI /URI (https://jnnp.bmj.com/content/56/1/98.full.pdf+html) >> endobj 451 0 obj << /Type /Annot /Subtype /Link /A 452 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 570.5280 91.2600 578.1592 ] >> endobj 452 0 obj << /Type /Action /S /URI /URI (https://ukcatalogue.oup.com/product/9780198510604.do) >> endobj 453 0 obj << /Type /Annot /Subtype /Link /A 454 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 543.4920 91.2600 551.1232 ] >> endobj 454 0 obj << /Type /Action /S /URI /URI (https://www.hindawi.com/journals/drt/2011/893905/) >> endobj 455 0 obj << /Type /Annot /Subtype /Link /A 456 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 516.4560 91.2600 524.0873 ] >> endobj 456 0 obj << /Type /Action /S /URI /URI (https://ajp.psychiatryonline.org/article.aspx?volume=140&page=728) >> endobj 457 0 obj << /Type /Annot /Subtype /Link /A 458 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 477.5152 91.2600 485.1465 ] >> endobj 458 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/016517819190040V) >> endobj 459 0 obj << /Type /Annot /Subtype /Link /A 460 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 450.4792 91.2600 458.1105 ] >> endobj 460 0 obj << /Type /Action /S /URI /URI (https://neuro.psychiatryonline.org/article.aspx?volume=3&page=243) >> endobj 461 0 obj << /Type /Annot /Subtype /Link /A 462 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 411.5385 91.2600 419.1697 ] >> endobj 462 0 obj << /Type /Action /S /URI /URI (https://www.jpsychores.com/article/S0022-3999\(10\)00017-6/abstract) >> endobj 463 0 obj << /Type /Annot /Subtype /Link /A 464 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 372.5977 91.2600 380.2290 ] >> endobj 464 0 obj << /Type /Action /S /URI /URI (https://www.springerlink.com/content/dwm51828866p8522/) >> endobj 465 0 obj << /Type /Annot /Subtype /Link /A 466 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 333.6570 91.2600 341.2882 ] >> endobj 466 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1986826/) >> endobj 467 0 obj << /Type /Annot /Subtype /Link /A 468 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 294.7162 91.2600 302.3475 ] >> endobj 468 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005986/?tool=pubmed) >> endobj 469 0 obj << /Type /Annot /Subtype /Link /A 470 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 255.7755 91.2600 263.4067 ] >> endobj 470 0 obj << /Type /Action /S /URI /URI (https://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=7133780) >> endobj 471 0 obj << /Type /Annot /Subtype /Link /A 472 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 216.8347 91.2600 224.4660 ] >> endobj 472 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0091305796004455) >> endobj 473 0 obj << /Type /Annot /Subtype /Link /A 474 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 189.7987 91.2600 197.4300 ] >> endobj 474 0 obj << /Type /Action /S /URI /URI (https://www.sciencemag.org/content/134/3483/943.short) >> endobj 475 0 obj << /Type /Annot /Subtype /Link /A 476 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 150.8580 91.2600 158.4892 ] >> endobj 476 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806234/?tool=pubmed) >> endobj 477 0 obj << /Type /Annot /Subtype /Link /A 478 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 111.9172 91.2600 119.5485 ] >> endobj 478 0 obj << /Type /Action /S /URI /URI (https://www.jneurosci.org/content/28/24/6182.short) >> endobj 479 0 obj << /Type /Annot /Subtype /Link /A 480 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 72.9765 91.2600 80.6077 ] >> endobj 480 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/neuron/retrieve/pii/S0896627300807643) >> endobj 481 0 obj << /Type /Annot /Subtype /Link /A 482 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 609.4688 91.2600 617.1000 ] >> endobj 482 0 obj << /Type /Action /S /URI /URI (https://jnnp.bmj.com/content/56/1/98.full.pdf+html) >> endobj 483 0 obj << /Type /Annot /Subtype /Link /A 484 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 570.5280 91.2600 578.1592 ] >> endobj 484 0 obj << /Type /Action /S /URI /URI (https://ukcatalogue.oup.com/product/9780198510604.do) >> endobj 485 0 obj << /Type /Annot /Subtype /Link /A 486 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 543.4920 91.2600 551.1232 ] >> endobj 486 0 obj << /Type /Action /S /URI /URI (https://www.hindawi.com/journals/drt/2011/893905/) >> endobj 487 0 obj << /Type /Annot /Subtype /Link /A 488 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 516.4560 91.2600 524.0873 ] >> endobj 488 0 obj << /Type /Action /S /URI /URI (https://ajp.psychiatryonline.org/article.aspx?volume=140&page=728) >> endobj 489 0 obj << /Type /Annot /Subtype /Link /A 490 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 477.5152 91.2600 485.1465 ] >> endobj 490 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/016517819190040V) >> endobj 491 0 obj << /Type /Annot /Subtype /Link /A 492 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 450.4792 91.2600 458.1105 ] >> endobj 492 0 obj << /Type /Action /S /URI /URI (https://neuro.psychiatryonline.org/article.aspx?volume=3&page=243) >> endobj 493 0 obj << /Type /Annot /Subtype /Link /A 494 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 411.5385 91.2600 419.1697 ] >> endobj 494 0 obj << /Type /Action /S /URI /URI (https://www.jpsychores.com/article/S0022-3999\(10\)00017-6/abstract) >> endobj 495 0 obj << /Type /Annot /Subtype /Link /A 496 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 372.5977 91.2600 380.2290 ] >> endobj 496 0 obj << /Type /Action /S /URI /URI (https://www.springerlink.com/content/dwm51828866p8522/) >> endobj 497 0 obj << /Type /Annot /Subtype /Link /A 498 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 333.6570 91.2600 341.2882 ] >> endobj 498 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1986826/) >> endobj 499 0 obj << /Type /Annot /Subtype /Link /A 500 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 294.7162 91.2600 302.3475 ] >> endobj 500 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005986/?tool=pubmed) >> endobj 501 0 obj << /Type /Annot /Subtype /Link /A 502 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 255.7755 91.2600 263.4067 ] >> endobj 502 0 obj << /Type /Action /S /URI /URI (https://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=7133780) >> endobj 503 0 obj << /Type /Annot /Subtype /Link /A 504 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 216.8347 91.2600 224.4660 ] >> endobj 504 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0091305796004455) >> endobj 505 0 obj << /Type /Annot /Subtype /Link /A 506 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 189.7987 91.2600 197.4300 ] >> endobj 506 0 obj << /Type /Action /S /URI /URI (https://www.sciencemag.org/content/134/3483/943.short) >> endobj 507 0 obj << /Type /Annot /Subtype /Link /A 508 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 150.8580 91.2600 158.4892 ] >> endobj 508 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806234/?tool=pubmed) >> endobj 509 0 obj << /Type /Annot /Subtype /Link /A 510 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 111.9172 91.2600 119.5485 ] >> endobj 510 0 obj << /Type /Action /S /URI /URI (https://www.jneurosci.org/content/28/24/6182.short) >> endobj 511 0 obj << /Type /Annot /Subtype /Link /A 512 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 72.9765 91.2600 80.6077 ] >> endobj 512 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/neuron/retrieve/pii/S0896627300807643) >> endobj 513 0 obj << /Type /Page /Parent 3 0 R /Annots [ 515 0 R 517 0 R 519 0 R 521 0 R 523 0 R 525 0 R 527 0 R 529 0 R 531 0 R 533 0 R 535 0 R 537 0 R 539 0 R 541 0 R 543 0 R ] /Contents 514 0 R >> endobj 514 0 obj << /Length 7100 >> stream 0.271 0.267 0.267 rg q 15.000 555.391 577.500 221.608 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(repeat is sufficient to cause a progressive neurological phenotype in transgenic mice. Cell 87: 493-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 758.990 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 740.440 Td /F1 9.8 Tf [(18.)] TJ ET BT 43.553 740.440 Td /F1 9.8 Tf [(Menalled LB, Sison JD, Dragatsis I, Zeitlin S, Chesselet MF \(2003\) Time course of early motor and neuropathological )] TJ ET BT 26.250 728.535 Td /F1 9.8 Tf [(anomalies in a knock-in mouse model of Huntington's disease with 140 CAG repeats. J Comp Neurol 465: 11-26.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 720.049 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 701.499 Td /F1 9.8 Tf [(19.)] TJ ET BT 43.553 701.499 Td /F1 9.8 Tf [(Menalled L, Kudwa A, Miller S, Fitzpatrick J, Watson-Johnson J, et al. \(in prep.\) Comprehensive behavioral characterization )] TJ ET BT 26.250 689.595 Td /F1 9.8 Tf [(of a new knock in mouse model of Huntingtons disease.)] TJ ET BT 26.250 670.190 Td /F1 9.8 Tf [(20.)] TJ ET BT 43.553 670.190 Td /F1 9.8 Tf [( Oakeshott S, Balci F, Filippov I, Murphy C, Port R, et al. \(2011\) Circadian Abnormalities in Motor Activity in a BAC )] TJ ET BT 26.250 658.285 Td /F1 9.8 Tf [(Transgenic Mouse Model of Huntingtons Disease. PLoS Curr. 2011 April 5; 3: RRN1225.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 649.799 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 631.249 Td /F1 9.8 Tf [(21.)] TJ ET BT 43.553 631.249 Td /F1 9.8 Tf [(Singer J \(1998\) Using SAS PROC MIXED to Fit Multilevel Models, Hierarchical Models, and Individual Growth Models. )] TJ ET BT 26.250 619.344 Td /F1 9.8 Tf [(Journal of Educational and Behavioral Statistics 23: 323.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.859 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 592.308 Td /F1 9.8 Tf [(22.)] TJ ET BT 43.553 592.308 Td /F1 9.8 Tf [(Trueman RC, Dunnett SB, Brooks SP \(2011\) Operant-based instrumental learning for analysis of genetically modified )] TJ ET BT 26.250 580.404 Td /F1 9.8 Tf [(models of Huntington's disease. Brain Res Bull.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.918 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET Q q 15.000 555.391 577.500 221.608 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(repeat is sufficient to cause a progressive neurological phenotype in transgenic mice. Cell 87: 493-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 758.990 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 740.440 Td /F1 9.8 Tf [(18.)] TJ ET BT 43.553 740.440 Td /F1 9.8 Tf [(Menalled LB, Sison JD, Dragatsis I, Zeitlin S, Chesselet MF \(2003\) Time course of early motor and neuropathological )] TJ ET BT 26.250 728.535 Td /F1 9.8 Tf [(anomalies in a knock-in mouse model of Huntington's disease with 140 CAG repeats. J Comp Neurol 465: 11-26.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 720.049 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 701.499 Td /F1 9.8 Tf [(19.)] TJ ET BT 43.553 701.499 Td /F1 9.8 Tf [(Menalled L, Kudwa A, Miller S, Fitzpatrick J, Watson-Johnson J, et al. \(in prep.\) Comprehensive behavioral characterization )] TJ ET BT 26.250 689.595 Td /F1 9.8 Tf [(of a new knock in mouse model of Huntingtons disease.)] TJ ET BT 26.250 670.190 Td /F1 9.8 Tf [(20.)] TJ ET BT 43.553 670.190 Td /F1 9.8 Tf [( Oakeshott S, Balci F, Filippov I, Murphy C, Port R, et al. \(2011\) Circadian Abnormalities in Motor Activity in a BAC )] TJ ET BT 26.250 658.285 Td /F1 9.8 Tf [(Transgenic Mouse Model of Huntingtons Disease. PLoS Curr. 2011 April 5; 3: RRN1225.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 649.799 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 631.249 Td /F1 9.8 Tf [(21.)] TJ ET BT 43.553 631.249 Td /F1 9.8 Tf [(Singer J \(1998\) Using SAS PROC MIXED to Fit Multilevel Models, Hierarchical Models, and Individual Growth Models. )] TJ ET BT 26.250 619.344 Td /F1 9.8 Tf [(Journal of Educational and Behavioral Statistics 23: 323.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.859 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 592.308 Td /F1 9.8 Tf [(22.)] TJ ET BT 43.553 592.308 Td /F1 9.8 Tf [(Trueman RC, Dunnett SB, Brooks SP \(2011\) Operant-based instrumental learning for analysis of genetically modified )] TJ ET BT 26.250 580.404 Td /F1 9.8 Tf [(models of Huntington's disease. Brain Res Bull.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.918 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET Q q 15.000 555.391 577.500 221.608 re W n 0.271 0.267 0.267 rg BT 26.250 767.476 Td /F1 9.8 Tf [(repeat is sufficient to cause a progressive neurological phenotype in transgenic mice. Cell 87: 493-506.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 758.990 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 740.440 Td /F1 9.8 Tf [(18.)] TJ ET BT 43.553 740.440 Td /F1 9.8 Tf [(Menalled LB, Sison JD, Dragatsis I, Zeitlin S, Chesselet MF \(2003\) Time course of early motor and neuropathological )] TJ ET BT 26.250 728.535 Td /F1 9.8 Tf [(anomalies in a knock-in mouse model of Huntington's disease with 140 CAG repeats. J Comp Neurol 465: 11-26.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 720.049 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 701.499 Td /F1 9.8 Tf [(19.)] TJ ET BT 43.553 701.499 Td /F1 9.8 Tf [(Menalled L, Kudwa A, Miller S, Fitzpatrick J, Watson-Johnson J, et al. \(in prep.\) Comprehensive behavioral characterization )] TJ ET BT 26.250 689.595 Td /F1 9.8 Tf [(of a new knock in mouse model of Huntingtons disease.)] TJ ET BT 26.250 670.190 Td /F1 9.8 Tf [(20.)] TJ ET BT 43.553 670.190 Td /F1 9.8 Tf [( Oakeshott S, Balci F, Filippov I, Murphy C, Port R, et al. \(2011\) Circadian Abnormalities in Motor Activity in a BAC )] TJ ET BT 26.250 658.285 Td /F1 9.8 Tf [(Transgenic Mouse Model of Huntingtons Disease. PLoS Curr. 2011 April 5; 3: RRN1225.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 649.799 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 631.249 Td /F1 9.8 Tf [(21.)] TJ ET BT 43.553 631.249 Td /F1 9.8 Tf [(Singer J \(1998\) Using SAS PROC MIXED to Fit Multilevel Models, Hierarchical Models, and Individual Growth Models. )] TJ ET BT 26.250 619.344 Td /F1 9.8 Tf [(Journal of Educational and Behavioral Statistics 23: 323.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 610.859 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET 0.271 0.267 0.267 rg BT 26.250 592.308 Td /F1 9.8 Tf [(22.)] TJ ET BT 43.553 592.308 Td /F1 9.8 Tf [(Trueman RC, Dunnett SB, Brooks SP \(2011\) Operant-based instrumental learning for analysis of genetically modified )] TJ ET BT 26.250 580.404 Td /F1 9.8 Tf [(models of Huntington's disease. Brain Res Bull.)] TJ ET 0.267 0.267 0.267 rg BT 26.250 571.918 Td /F1 7.5 Tf [(REFERENCE LINK)] TJ ET Q q 0.000 0.000 0.000 rg BT 291.710 19.825 Td /F1 11.0 Tf [(11)] TJ ET BT 25.000 19.825 Td /F1 11.0 Tf [(PLOS Currents Huntington Disease)] TJ ET Q endstream endobj 515 0 obj << /Type /Annot /Subtype /Link /A 516 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 758.2965 91.2600 765.9277 ] >> endobj 516 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/abstract/S0092-8674\(00\)81369-0) >> endobj 517 0 obj << /Type /Annot /Subtype /Link /A 518 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 719.3557 91.2600 726.9870 ] >> endobj 518 0 obj << /Type /Action /S /URI /URI (https://onlinelibrary.wiley.com/doi/10.1002/cne.10776/abstract;jsessionid=FF34DE99F0655B309EC820CB297D0481.d03t01) >> endobj 519 0 obj << /Type /Annot /Subtype /Link /A 520 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 649.1055 91.2600 656.7368 ] >> endobj 520 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072044/) >> endobj 521 0 obj << /Type /Annot /Subtype /Link /A 522 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 610.1648 91.2600 617.7960 ] >> endobj 522 0 obj << /Type /Action /S /URI /URI (https://jeb.sagepub.com/content/23/4/323.short?rss=1&ssource=mfc) >> endobj 523 0 obj << /Type /Annot /Subtype /Link /A 524 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 571.2240 91.2600 578.8552 ] >> endobj 524 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0361923011001031) >> endobj 525 0 obj << /Type /Annot /Subtype /Link /A 526 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 758.2965 91.2600 765.9277 ] >> endobj 526 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/abstract/S0092-8674\(00\)81369-0) >> endobj 527 0 obj << /Type /Annot /Subtype /Link /A 528 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 719.3557 91.2600 726.9870 ] >> endobj 528 0 obj << /Type /Action /S /URI /URI (https://onlinelibrary.wiley.com/doi/10.1002/cne.10776/abstract;jsessionid=FF34DE99F0655B309EC820CB297D0481.d03t01) >> endobj 529 0 obj << /Type /Annot /Subtype /Link /A 530 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 649.1055 91.2600 656.7368 ] >> endobj 530 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072044/) >> endobj 531 0 obj << /Type /Annot /Subtype /Link /A 532 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 610.1648 91.2600 617.7960 ] >> endobj 532 0 obj << /Type /Action /S /URI /URI (https://jeb.sagepub.com/content/23/4/323.short?rss=1&ssource=mfc) >> endobj 533 0 obj << /Type /Annot /Subtype /Link /A 534 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 571.2240 91.2600 578.8552 ] >> endobj 534 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0361923011001031) >> endobj 535 0 obj << /Type /Annot /Subtype /Link /A 536 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 758.2965 91.2600 765.9277 ] >> endobj 536 0 obj << /Type /Action /S /URI /URI (https://www.cell.com/abstract/S0092-8674\(00\)81369-0) >> endobj 537 0 obj << /Type /Annot /Subtype /Link /A 538 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 719.3557 91.2600 726.9870 ] >> endobj 538 0 obj << /Type /Action /S /URI /URI (https://onlinelibrary.wiley.com/doi/10.1002/cne.10776/abstract;jsessionid=FF34DE99F0655B309EC820CB297D0481.d03t01) >> endobj 539 0 obj << /Type /Annot /Subtype /Link /A 540 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 649.1055 91.2600 656.7368 ] >> endobj 540 0 obj << /Type /Action /S /URI /URI (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072044/) >> endobj 541 0 obj << /Type /Annot /Subtype /Link /A 542 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 610.1648 91.2600 617.7960 ] >> endobj 542 0 obj << /Type /Action /S /URI /URI (https://jeb.sagepub.com/content/23/4/323.short?rss=1&ssource=mfc) >> endobj 543 0 obj << /Type /Annot /Subtype /Link /A 544 0 R /Border [0 0 0] /H /I /Rect [ 26.2500 571.2240 91.2600 578.8552 ] >> endobj 544 0 obj << /Type /Action /S /URI /URI (https://www.sciencedirect.com/science/article/pii/S0361923011001031) >> endobj xref 0 545 0000000000 65535 f 0000000008 00000 n 0000000073 00000 n 0000000119 00000 n 0000001131 00000 n 0000001168 00000 n 0000001471 00000 n 0000002103 00000 n 0000029929 00000 n 0000030036 00000 n 0000030144 00000 n 0000030255 00000 n 0000030368 00000 n 0000030756 00000 n 0000035237 00000 n 0000035364 00000 n 0000035595 00000 n 0000035722 00000 n 0000035953 00000 n 0000036080 00000 n 0000036311 00000 n 0000036438 00000 n 0000036545 00000 n 0000036672 00000 n 0000036776 00000 n 0000036904 00000 n 0000037003 00000 n 0000037131 00000 n 0000037237 00000 n 0000037365 00000 n 0000037464 00000 n 0000037592 00000 n 0000037697 00000 n 0000037825 00000 n 0000037925 00000 n 0000038053 00000 n 0000038153 00000 n 0000038279 00000 n 0000038376 00000 n 0000038503 00000 n 0000038603 00000 n 0000038731 00000 n 0000038830 00000 n 0000038958 00000 n 0000038994 00000 n 0000039122 00000 n 0000039158 00000 n 0000039286 00000 n 0000039322 00000 n 0000039450 00000 n 0000039486 00000 n 0000039612 00000 n 0000039648 00000 n 0000039774 00000 n 0000039810 00000 n 0000039936 00000 n 0000039972 00000 n 0000040098 00000 n 0000040134 00000 n 0000040260 00000 n 0000040296 00000 n 0000040420 00000 n 0000040456 00000 n 0000040580 00000 n 0000040616 00000 n 0000040743 00000 n 0000040974 00000 n 0000041101 00000 n 0000041332 00000 n 0000041459 00000 n 0000041690 00000 n 0000041817 00000 n 0000041924 00000 n 0000042051 00000 n 0000042155 00000 n 0000042283 00000 n 0000042382 00000 n 0000042510 00000 n 0000042616 00000 n 0000042744 00000 n 0000042843 00000 n 0000042971 00000 n 0000043076 00000 n 0000043204 00000 n 0000043304 00000 n 0000043432 00000 n 0000043532 00000 n 0000043658 00000 n 0000043755 00000 n 0000043882 00000 n 0000043982 00000 n 0000044110 00000 n 0000044209 00000 n 0000044337 00000 n 0000044373 00000 n 0000044501 00000 n 0000044537 00000 n 0000044665 00000 n 0000044701 00000 n 0000044829 00000 n 0000044865 00000 n 0000044993 00000 n 0000045030 00000 n 0000045158 00000 n 0000045195 00000 n 0000045323 00000 n 0000045360 00000 n 0000045488 00000 n 0000045525 00000 n 0000045653 00000 n 0000045690 00000 n 0000045816 00000 n 0000045853 00000 n 0000045979 00000 n 0000046016 00000 n 0000046145 00000 n 0000046377 00000 n 0000046506 00000 n 0000046738 00000 n 0000046867 00000 n 0000047099 00000 n 0000047228 00000 n 0000047336 00000 n 0000047465 00000 n 0000047570 00000 n 0000047700 00000 n 0000047800 00000 n 0000047930 00000 n 0000048037 00000 n 0000048167 00000 n 0000048267 00000 n 0000048397 00000 n 0000048503 00000 n 0000048633 00000 n 0000048734 00000 n 0000048864 00000 n 0000048965 00000 n 0000049093 00000 n 0000049191 00000 n 0000049320 00000 n 0000049421 00000 n 0000049551 00000 n 0000049651 00000 n 0000049781 00000 n 0000049818 00000 n 0000049948 00000 n 0000049985 00000 n 0000050115 00000 n 0000050152 00000 n 0000050282 00000 n 0000050319 00000 n 0000050447 00000 n 0000050484 00000 n 0000050612 00000 n 0000050649 00000 n 0000050777 00000 n 0000050814 00000 n 0000050942 00000 n 0000050979 00000 n 0000051107 00000 n 0000051144 00000 n 0000051270 00000 n 0000051307 00000 n 0000051433 00000 n 0000051470 00000 n 0000051885 00000 n 0000079205 00000 n 0000079322 00000 n 0000079450 00000 n 0000079487 00000 n 0000079617 00000 n 0000079654 00000 n 0000079784 00000 n 0000079821 00000 n 0000079949 00000 n 0000079986 00000 n 0000080116 00000 n 0000080153 00000 n 0000080283 00000 n 0000080320 00000 n 0000080450 00000 n 0000080487 00000 n 0000080617 00000 n 0000080654 00000 n 0000080784 00000 n 0000080821 00000 n 0000080951 00000 n 0000080988 00000 n 0000081118 00000 n 0000081155 00000 n 0000081285 00000 n 0000081322 00000 n 0000081451 00000 n 0000081488 00000 n 0000081618 00000 n 0000081655 00000 n 0000081783 00000 n 0000081820 00000 n 0000081950 00000 n 0000081987 00000 n 0000082117 00000 n 0000082154 00000 n 0000082282 00000 n 0000082319 00000 n 0000082449 00000 n 0000082486 00000 n 0000082616 00000 n 0000082653 00000 n 0000082783 00000 n 0000082820 00000 n 0000082950 00000 n 0000082987 00000 n 0000083117 00000 n 0000083154 00000 n 0000083284 00000 n 0000083321 00000 n 0000083451 00000 n 0000083488 00000 n 0000083618 00000 n 0000083655 00000 n 0000083784 00000 n 0000083821 00000 n 0000083951 00000 n 0000083988 00000 n 0000084116 00000 n 0000084153 00000 n 0000084283 00000 n 0000084320 00000 n 0000084450 00000 n 0000084487 00000 n 0000084615 00000 n 0000084652 00000 n 0000084782 00000 n 0000084819 00000 n 0000084949 00000 n 0000084986 00000 n 0000085116 00000 n 0000085153 00000 n 0000085283 00000 n 0000085320 00000 n 0000085450 00000 n 0000085487 00000 n 0000085617 00000 n 0000085654 00000 n 0000085784 00000 n 0000085821 00000 n 0000085951 00000 n 0000085988 00000 n 0000086117 00000 n 0000086154 00000 n 0000086284 00000 n 0000086321 00000 n 0000086424 00000 n 0000108807 00000 n 0000108936 00000 n 0000109042 00000 n 0000109543 00000 n 0000135430 00000 n 0000135559 00000 n 0000135665 00000 n 0000136166 00000 n 0000162053 00000 n 0000162182 00000 n 0000162288 00000 n 0000162789 00000 n 0000188676 00000 n 0000189177 00000 n 0000215064 00000 n 0000215191 00000 n 0000228595 00000 n 0000228724 00000 n 0000228830 00000 n 0000229557 00000 n 0000276194 00000 n 0000276323 00000 n 0000276431 00000 n 0000277158 00000 n 0000325401 00000 n 0000325530 00000 n 0000325636 00000 n 0000326363 00000 n 0000373000 00000 n 0000373129 00000 n 0000373237 00000 n 0000373964 00000 n 0000422207 00000 n 0000422336 00000 n 0000422442 00000 n 0000423169 00000 n 0000469806 00000 n 0000469935 00000 n 0000470043 00000 n 0000470770 00000 n 0000519013 00000 n 0000519740 00000 n 0000566377 00000 n 0000567104 00000 n 0000615347 00000 n 0000615474 00000 n 0000637406 00000 n 0000637532 00000 n 0000637569 00000 n 0000637697 00000 n 0000637734 00000 n 0000637860 00000 n 0000637897 00000 n 0000638025 00000 n 0000638062 00000 n 0000638188 00000 n 0000638225 00000 n 0000638353 00000 n 0000638390 00000 n 0000638517 00000 n 0000656170 00000 n 0000656299 00000 n 0000656405 00000 n 0000656906 00000 n 0000677173 00000 n 0000677301 00000 n 0000677407 00000 n 0000678134 00000 n 0000719659 00000 n 0000719788 00000 n 0000719894 00000 n 0000720395 00000 n 0000740662 00000 n 0000740790 00000 n 0000740896 00000 n 0000741623 00000 n 0000783148 00000 n 0000783277 00000 n 0000783383 00000 n 0000783884 00000 n 0000804151 00000 n 0000804279 00000 n 0000804385 00000 n 0000805112 00000 n 0000846637 00000 n 0000847138 00000 n 0000867405 00000 n 0000868132 00000 n 0000909657 00000 n 0000909760 00000 n 0000924923 00000 n 0000925052 00000 n 0000925160 00000 n 0000925887 00000 n 0000974130 00000 n 0000974259 00000 n 0000974367 00000 n 0000975094 00000 n 0001023337 00000 n 0001023466 00000 n 0001023574 00000 n 0001024301 00000 n 0001072544 00000 n 0001073271 00000 n 0001121514 00000 n 0001121581 00000 n 0001143705 00000 n 0001144469 00000 n 0001224389 00000 n 0001225153 00000 n 0001305073 00000 n 0001305837 00000 n 0001385757 00000 n 0001386028 00000 n 0001416300 00000 n 0001416428 00000 n 0001416465 00000 n 0001416595 00000 n 0001416632 00000 n 0001416760 00000 n 0001416797 00000 n 0001416927 00000 n 0001416964 00000 n 0001417094 00000 n 0001417131 00000 n 0001417261 00000 n 0001417298 00000 n 0001417428 00000 n 0001417465 00000 n 0001417595 00000 n 0001417632 00000 n 0001417760 00000 n 0001417797 00000 n 0001417927 00000 n 0001417964 00000 n 0001418092 00000 n 0001418129 00000 n 0001418259 00000 n 0001418296 00000 n 0001418426 00000 n 0001418463 00000 n 0001418593 00000 n 0001418630 00000 n 0001418760 00000 n 0001418797 00000 n 0001418927 00000 n 0001418964 00000 n 0001419092 00000 n 0001419129 00000 n 0001419259 00000 n 0001419296 00000 n 0001419424 00000 n 0001419461 00000 n 0001419591 00000 n 0001419628 00000 n 0001419758 00000 n 0001419795 00000 n 0001419925 00000 n 0001419962 00000 n 0001420092 00000 n 0001420129 00000 n 0001420259 00000 n 0001420296 00000 n 0001420759 00000 n 0001442128 00000 n 0001442256 00000 n 0001442358 00000 n 0001442486 00000 n 0001442590 00000 n 0001442718 00000 n 0001442819 00000 n 0001442947 00000 n 0001443064 00000 n 0001443192 00000 n 0001443310 00000 n 0001443438 00000 n 0001443555 00000 n 0001443683 00000 n 0001443802 00000 n 0001443930 00000 n 0001444036 00000 n 0001444164 00000 n 0001444269 00000 n 0001444397 00000 n 0001444514 00000 n 0001444642 00000 n 0001444775 00000 n 0001444903 00000 n 0001445022 00000 n 0001445150 00000 n 0001445255 00000 n 0001445383 00000 n 0001445500 00000 n 0001445628 00000 n 0001445730 00000 n 0001445856 00000 n 0001445966 00000 n 0001446094 00000 n 0001446196 00000 n 0001446324 00000 n 0001446428 00000 n 0001446556 00000 n 0001446657 00000 n 0001446785 00000 n 0001446902 00000 n 0001447030 00000 n 0001447148 00000 n 0001447276 00000 n 0001447393 00000 n 0001447521 00000 n 0001447640 00000 n 0001447768 00000 n 0001447874 00000 n 0001448002 00000 n 0001448107 00000 n 0001448235 00000 n 0001448352 00000 n 0001448480 00000 n 0001448613 00000 n 0001448741 00000 n 0001448860 00000 n 0001448988 00000 n 0001449093 00000 n 0001449221 00000 n 0001449338 00000 n 0001449466 00000 n 0001449568 00000 n 0001449694 00000 n 0001449804 00000 n 0001449932 00000 n 0001450034 00000 n 0001450162 00000 n 0001450266 00000 n 0001450394 00000 n 0001450495 00000 n 0001450623 00000 n 0001450740 00000 n 0001450868 00000 n 0001450986 00000 n 0001451114 00000 n 0001451231 00000 n 0001451359 00000 n 0001451478 00000 n 0001451606 00000 n 0001451712 00000 n 0001451840 00000 n 0001451945 00000 n 0001452073 00000 n 0001452190 00000 n 0001452318 00000 n 0001452451 00000 n 0001452579 00000 n 0001452698 00000 n 0001452826 00000 n 0001452931 00000 n 0001453059 00000 n 0001453176 00000 n 0001453304 00000 n 0001453406 00000 n 0001453532 00000 n 0001453642 00000 n 0001453841 00000 n 0001460995 00000 n 0001461123 00000 n 0001461228 00000 n 0001461356 00000 n 0001461521 00000 n 0001461649 00000 n 0001461754 00000 n 0001461882 00000 n 0001461998 00000 n 0001462126 00000 n 0001462245 00000 n 0001462373 00000 n 0001462478 00000 n 0001462606 00000 n 0001462771 00000 n 0001462899 00000 n 0001463004 00000 n 0001463132 00000 n 0001463248 00000 n 0001463376 00000 n 0001463495 00000 n 0001463623 00000 n 0001463728 00000 n 0001463856 00000 n 0001464021 00000 n 0001464149 00000 n 0001464254 00000 n 0001464382 00000 n 0001464498 00000 n 0001464626 00000 n trailer << /Size 545 /Root 1 0 R /Info 5 0 R >> startxref 1464745 %%EOF