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BT 15.000 718.042 Td /F2 21.0 Tf  [(Symptomatic infections less frequent with H1N1pdm than with )] TJ ET
BT 15.000 693.094 Td /F2 21.0 Tf  [(seasonal strains)] TJ ET
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BT 101.375 675.088 Td /F3 9.8 Tf  [(Influenza)] TJ ET
BT 26.250 663.247 Td /F1 9.8 Tf  [(Antoine FLAHAULT)] TJ ET
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BT 111.865 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
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BT 230.532 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
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BT 235.953 663.247 Td /F1 9.8 Tf  [(Thomas Hanslik)] TJ ET
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BT 305.841 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
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BT 311.262 663.247 Td /F1 9.8 Tf  [(nicolas salez)] TJ ET
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BT 26.250 651.342 Td /F1 9.8 Tf  [(FLAHAULT A, DE LAMBALLERIE X, Hanslik T, salez n. Symptomatic infections less frequent with H1N1pdm than with )] TJ ET
BT 26.250 639.438 Td /F1 9.8 Tf  [(seasonal strains. PLOS Currents Influenza. 2009 Dec 27 . Edition 1. doi: 10.1371/currents.RRN1140.)] TJ ET
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BT 26.250 610.335 Td /F4 12.0 Tf  [(Abstract)] TJ ET
BT 26.250 590.381 Td /F1 9.8 Tf  [(A serosurvey conducted in a sample of first quarter pregnant women in France at week 48-49 of 2009 exhibit a seroprevalence )] TJ ET
BT 26.250 578.476 Td /F1 9.8 Tf  [(level of 10.6%. It has been extrapolated in male and female population living in France mainland, aged 20-39 yr, that 1,712,000, )] TJ ET
BT 26.250 566.571 Td /F1 9.8 Tf  [(95%CI \(1,112,700 � 2,311,300\) people were recently infected by H1N1pdm \(recently vaccinated women were excluded from )] TJ ET
BT 26.250 554.667 Td /F1 9.8 Tf  [(analysis\). From week 36 to 46-47 of 2009, 336,288, 95%CI \(207,303-421,299\) patients visited their general practitioners with )] TJ ET
BT 26.250 542.762 Td /F1 9.8 Tf  [(clinical influenza in France, mainland. We then extrapolated the proportion of symptomatic H1N1pdm influenza in both males )] TJ ET
BT 26.250 530.857 Td /F1 9.8 Tf  [(and females aged 20-39 yr who visited their GP to be 19.6%. Surprisingly, 8 months after having alerted at a global level for )] TJ ET
BT 26.250 518.952 Td /F1 9.8 Tf  [(H1N1pdm, there is still no published data on seroprevalence in any countries. Therefore, there is still no published estimation of )] TJ ET
BT 26.250 507.048 Td /F1 9.8 Tf  [(the proportion of asymptomatic infections due to the pandemic strain. We have launched in France a weekly repeated )] TJ ET
BT 26.250 495.143 Td /F1 9.8 Tf  [(seroprevalence survey in pregnant women who were volunteers to give their blood for H1N1pdm hemagglutination inhibition )] TJ ET
BT 26.250 483.238 Td /F1 9.8 Tf  [(\(HI\) serosurvey during their first term of their pregnancy [1] . By comparing our data to cumulated figures of incident cases of )] TJ ET
BT 26.250 471.333 Td /F1 9.8 Tf  [(clinical influenza provided by the French Sentinelles system [2] we provide estimate of the proportion of patients with )] TJ ET
BT 26.250 459.429 Td /F1 9.8 Tf  [(symptomatic influenza presentation which led them to visiting their general practitioner.)] TJ ET
BT 26.250 422.826 Td /F4 12.0 Tf  [(Funding Statement)] TJ ET
BT 26.250 402.872 Td /F1 9.8 Tf  [(This work is funded by EHESP School of Public Health, Rennes, France.)] TJ ET
BT 26.250 373.769 Td /F4 12.0 Tf  [(Weekly repeated H1N1pdm seroprevalence survey)] TJ ET
BT 26.250 353.815 Td /F1 9.8 Tf  [(We have launched a regular seroprevalence survey among a sample of consecutive pregnant women who systematically visit a )] TJ ET
BT 26.250 341.910 Td /F1 9.8 Tf  [(laboratory from a predefined network of labs for giving their blood for toxoplasmosis serology. These women gave a written )] TJ ET
BT 26.250 330.006 Td /F1 9.8 Tf  [(consent for providing a 50 microlitres serum sample for H1N1pdm serosurvey. Data on age and recent exposure to H1N1pdm )] TJ ET
BT 26.250 318.101 Td /F1 9.8 Tf  [(vaccination were collected. Blood samples were then shipped to Marseilles by express mail, where a standard HI assay using )] TJ ET
BT 26.250 306.196 Td /F1 9.8 Tf  [(human group O erythrocytes and 4 HI units of non inactivated H1N1pdm virus was performed in a L3 laboratory. Based on )] TJ ET
BT 26.250 294.291 Td /F1 9.8 Tf  [(results of previous tests performed in a sample of the general population and on the follow up of microbiologically documented )] TJ ET
BT 26.250 282.387 Td /F1 9.8 Tf  [(infections \(manuscript in preparation\), only dilution above 1/40 were considered to be relevant for detection of recent H1N1pdm )] TJ ET
BT 26.250 270.482 Td /F1 9.8 Tf  [(infections in this age group. From serological results obtained at weeks 48-49 in pregnant women, a cumulative seroprevalence )] TJ ET
BT 26.250 258.577 Td /F1 9.8 Tf  [(in both males and females aged 20-39 yr was estimated: 1,712,000 recent seroconversions to H1N1pdm, 95% confidence )] TJ ET
BT 26.250 246.672 Td /F1 9.8 Tf  [(interval \(1,112,700 ; 2,311,300\). These estimates are published on the InVS weekly bulletin )] TJ ET
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BT 422.412 246.672 Td /F1 9.8 Tf  [([3])] TJ ET
0.271 0.267 0.267 rg
BT 433.254 246.672 Td /F1 9.8 Tf  [(. Importantly, it should be noted )] TJ ET
BT 26.250 234.768 Td /F1 9.8 Tf  [(that, considering an observed delay of approximately 2 weeks for seroconversion, serological results obtained at weeks 48-49 )] TJ ET
BT 26.250 222.863 Td /F1 9.8 Tf  [(are representative of cumulated cases at week 46-47.)] TJ ET
BT 26.250 186.260 Td /F4 12.0 Tf  [(Clinical influenza in 20-39 yr from the French Sentinel system)] TJ ET
BT 26.250 166.306 Td /F1 9.8 Tf  [(The Sentinelles Network \(UMR-S 707, Inserm and University Pierre and Marie Curie\) is a network of 1300 volunteer general )] TJ ET
BT 26.250 154.401 Td /F1 9.8 Tf  [(practitioners, working throughout the metropolitan regions of France \(2% of the total general practitioners in these regions\). This )] TJ ET
BT 26.250 142.497 Td /F1 9.8 Tf  [(national electronic system of clinical surveillance permits the real-time surveillance of the incidence of influenza-like illness \(ILI\) )] TJ ET
BT 26.250 130.592 Td /F1 9.8 Tf  [(seen in general practice. A case of ILI is defined as a sudden fever > 39�C \(>102�F\) with myalgia and respiratory signs. Using a )] TJ ET
BT 26.250 118.687 Td /F1 9.8 Tf  [(periodic regression model applied to historical surveillance data, the excess consultations for influenza-like illness is estimated )] TJ ET
BT 26.250 106.782 Td /F1 9.8 Tf  [(each week. This excess is the difference between the observed number of consultations and the expected number, under the )] TJ ET
BT 26.250 94.878 Td /F1 9.8 Tf  [(hypothesis of no influenza circulation, estimated by the model. It can be ascribed to clinical influenza cases seen in general )] TJ ET
BT 26.250 82.973 Td /F1 9.8 Tf  [(practice.)] TJ ET
BT 26.250 63.568 Td /F1 9.8 Tf  [(Sentinelles provides an unrestricted access to its aggregated data )] TJ ET
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BT 313.475 63.568 Td /F1 9.8 Tf  [([4])] TJ ET
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BT 324.317 63.568 Td /F1 9.8 Tf  [(. However we had to customize our request by selecting )] TJ ET
BT 26.250 51.663 Td /F1 9.8 Tf  [(only cases aged 20 to 39 yr and to extrapolate for all France, under assumption of representativeness of the sample of general )] TJ ET
BT 26.250 39.759 Td /F1 9.8 Tf  [(practitioners. Cumulated extrapolations in this age group, from week 36 to week 46-47 of 2009 give the following figures: clinical )] TJ ET
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BT 101.375 675.088 Td /F3 9.8 Tf  [(Influenza)] TJ ET
BT 26.250 663.247 Td /F1 9.8 Tf  [(Antoine FLAHAULT)] TJ ET
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BT 111.865 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
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BT 117.286 663.247 Td /F1 9.8 Tf  [(Xavier DE LAMBALLERIE)] TJ ET
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BT 230.532 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
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BT 235.953 663.247 Td /F1 9.8 Tf  [(Thomas Hanslik)] TJ ET
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BT 305.841 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
0.267 0.267 0.267 rg
BT 311.262 663.247 Td /F1 9.8 Tf  [(nicolas salez)] TJ ET
0.271 0.267 0.267 rg
BT 26.250 651.342 Td /F1 9.8 Tf  [(FLAHAULT A, DE LAMBALLERIE X, Hanslik T, salez n. Symptomatic infections less frequent with H1N1pdm than with )] TJ ET
BT 26.250 639.438 Td /F1 9.8 Tf  [(seasonal strains. PLOS Currents Influenza. 2009 Dec 27 . Edition 1. doi: 10.1371/currents.RRN1140.)] TJ ET
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0.271 0.267 0.267 rg
BT 26.250 610.335 Td /F4 12.0 Tf  [(Abstract)] TJ ET
BT 26.250 590.381 Td /F1 9.8 Tf  [(A serosurvey conducted in a sample of first quarter pregnant women in France at week 48-49 of 2009 exhibit a seroprevalence )] TJ ET
BT 26.250 578.476 Td /F1 9.8 Tf  [(level of 10.6%. It has been extrapolated in male and female population living in France mainland, aged 20-39 yr, that 1,712,000, )] TJ ET
BT 26.250 566.571 Td /F1 9.8 Tf  [(95%CI \(1,112,700 � 2,311,300\) people were recently infected by H1N1pdm \(recently vaccinated women were excluded from )] TJ ET
BT 26.250 554.667 Td /F1 9.8 Tf  [(analysis\). From week 36 to 46-47 of 2009, 336,288, 95%CI \(207,303-421,299\) patients visited their general practitioners with )] TJ ET
BT 26.250 542.762 Td /F1 9.8 Tf  [(clinical influenza in France, mainland. We then extrapolated the proportion of symptomatic H1N1pdm influenza in both males )] TJ ET
BT 26.250 530.857 Td /F1 9.8 Tf  [(and females aged 20-39 yr who visited their GP to be 19.6%. Surprisingly, 8 months after having alerted at a global level for )] TJ ET
BT 26.250 518.952 Td /F1 9.8 Tf  [(H1N1pdm, there is still no published data on seroprevalence in any countries. Therefore, there is still no published estimation of )] TJ ET
BT 26.250 507.048 Td /F1 9.8 Tf  [(the proportion of asymptomatic infections due to the pandemic strain. We have launched in France a weekly repeated )] TJ ET
BT 26.250 495.143 Td /F1 9.8 Tf  [(seroprevalence survey in pregnant women who were volunteers to give their blood for H1N1pdm hemagglutination inhibition )] TJ ET
BT 26.250 483.238 Td /F1 9.8 Tf  [(\(HI\) serosurvey during their first term of their pregnancy [1] . By comparing our data to cumulated figures of incident cases of )] TJ ET
BT 26.250 471.333 Td /F1 9.8 Tf  [(clinical influenza provided by the French Sentinelles system [2] we provide estimate of the proportion of patients with )] TJ ET
BT 26.250 459.429 Td /F1 9.8 Tf  [(symptomatic influenza presentation which led them to visiting their general practitioner.)] TJ ET
BT 26.250 422.826 Td /F4 12.0 Tf  [(Funding Statement)] TJ ET
BT 26.250 402.872 Td /F1 9.8 Tf  [(This work is funded by EHESP School of Public Health, Rennes, France.)] TJ ET
BT 26.250 373.769 Td /F4 12.0 Tf  [(Weekly repeated H1N1pdm seroprevalence survey)] TJ ET
BT 26.250 353.815 Td /F1 9.8 Tf  [(We have launched a regular seroprevalence survey among a sample of consecutive pregnant women who systematically visit a )] TJ ET
BT 26.250 341.910 Td /F1 9.8 Tf  [(laboratory from a predefined network of labs for giving their blood for toxoplasmosis serology. These women gave a written )] TJ ET
BT 26.250 330.006 Td /F1 9.8 Tf  [(consent for providing a 50 microlitres serum sample for H1N1pdm serosurvey. Data on age and recent exposure to H1N1pdm )] TJ ET
BT 26.250 318.101 Td /F1 9.8 Tf  [(vaccination were collected. Blood samples were then shipped to Marseilles by express mail, where a standard HI assay using )] TJ ET
BT 26.250 306.196 Td /F1 9.8 Tf  [(human group O erythrocytes and 4 HI units of non inactivated H1N1pdm virus was performed in a L3 laboratory. Based on )] TJ ET
BT 26.250 294.291 Td /F1 9.8 Tf  [(results of previous tests performed in a sample of the general population and on the follow up of microbiologically documented )] TJ ET
BT 26.250 282.387 Td /F1 9.8 Tf  [(infections \(manuscript in preparation\), only dilution above 1/40 were considered to be relevant for detection of recent H1N1pdm )] TJ ET
BT 26.250 270.482 Td /F1 9.8 Tf  [(infections in this age group. From serological results obtained at weeks 48-49 in pregnant women, a cumulative seroprevalence )] TJ ET
BT 26.250 258.577 Td /F1 9.8 Tf  [(in both males and females aged 20-39 yr was estimated: 1,712,000 recent seroconversions to H1N1pdm, 95% confidence )] TJ ET
BT 26.250 246.672 Td /F1 9.8 Tf  [(interval \(1,112,700 ; 2,311,300\). These estimates are published on the InVS weekly bulletin )] TJ ET
0.267 0.267 0.267 rg
BT 422.412 246.672 Td /F1 9.8 Tf  [([3])] TJ ET
0.271 0.267 0.267 rg
BT 433.254 246.672 Td /F1 9.8 Tf  [(. Importantly, it should be noted )] TJ ET
BT 26.250 234.768 Td /F1 9.8 Tf  [(that, considering an observed delay of approximately 2 weeks for seroconversion, serological results obtained at weeks 48-49 )] TJ ET
BT 26.250 222.863 Td /F1 9.8 Tf  [(are representative of cumulated cases at week 46-47.)] TJ ET
BT 26.250 186.260 Td /F4 12.0 Tf  [(Clinical influenza in 20-39 yr from the French Sentinel system)] TJ ET
BT 26.250 166.306 Td /F1 9.8 Tf  [(The Sentinelles Network \(UMR-S 707, Inserm and University Pierre and Marie Curie\) is a network of 1300 volunteer general )] TJ ET
BT 26.250 154.401 Td /F1 9.8 Tf  [(practitioners, working throughout the metropolitan regions of France \(2% of the total general practitioners in these regions\). This )] TJ ET
BT 26.250 142.497 Td /F1 9.8 Tf  [(national electronic system of clinical surveillance permits the real-time surveillance of the incidence of influenza-like illness \(ILI\) )] TJ ET
BT 26.250 130.592 Td /F1 9.8 Tf  [(seen in general practice. A case of ILI is defined as a sudden fever > 39�C \(>102�F\) with myalgia and respiratory signs. Using a )] TJ ET
BT 26.250 118.687 Td /F1 9.8 Tf  [(periodic regression model applied to historical surveillance data, the excess consultations for influenza-like illness is estimated )] TJ ET
BT 26.250 106.782 Td /F1 9.8 Tf  [(each week. This excess is the difference between the observed number of consultations and the expected number, under the )] TJ ET
BT 26.250 94.878 Td /F1 9.8 Tf  [(hypothesis of no influenza circulation, estimated by the model. It can be ascribed to clinical influenza cases seen in general )] TJ ET
BT 26.250 82.973 Td /F1 9.8 Tf  [(practice.)] TJ ET
BT 26.250 63.568 Td /F1 9.8 Tf  [(Sentinelles provides an unrestricted access to its aggregated data )] TJ ET
0.267 0.267 0.267 rg
BT 313.475 63.568 Td /F1 9.8 Tf  [([4])] TJ ET
0.271 0.267 0.267 rg
BT 324.317 63.568 Td /F1 9.8 Tf  [(. However we had to customize our request by selecting )] TJ ET
BT 26.250 51.663 Td /F1 9.8 Tf  [(only cases aged 20 to 39 yr and to extrapolate for all France, under assumption of representativeness of the sample of general )] TJ ET
BT 26.250 39.759 Td /F1 9.8 Tf  [(practitioners. Cumulated extrapolations in this age group, from week 36 to week 46-47 of 2009 give the following figures: clinical )] TJ ET
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0.267 0.267 0.267 rg
BT 15.000 718.042 Td /F2 21.0 Tf  [(Symptomatic infections less frequent with H1N1pdm than with )] TJ ET
BT 15.000 693.094 Td /F2 21.0 Tf  [(seasonal strains)] TJ ET
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0.271 0.267 0.267 rg
BT 15.000 675.088 Td /F3 9.8 Tf  [(December 27, 2009)] TJ ET
BT 96.500 675.088 Td /F3 9.8 Tf  [(�)] TJ ET
0.267 0.267 0.267 rg
BT 101.375 675.088 Td /F3 9.8 Tf  [(Influenza)] TJ ET
BT 26.250 663.247 Td /F1 9.8 Tf  [(Antoine FLAHAULT)] TJ ET
0.271 0.267 0.267 rg
BT 111.865 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
0.267 0.267 0.267 rg
BT 117.286 663.247 Td /F1 9.8 Tf  [(Xavier DE LAMBALLERIE)] TJ ET
0.271 0.267 0.267 rg
BT 230.532 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
0.267 0.267 0.267 rg
BT 235.953 663.247 Td /F1 9.8 Tf  [(Thomas Hanslik)] TJ ET
0.271 0.267 0.267 rg
BT 305.841 663.247 Td /F1 9.8 Tf  [(, )] TJ ET
0.267 0.267 0.267 rg
BT 311.262 663.247 Td /F1 9.8 Tf  [(nicolas salez)] TJ ET
0.271 0.267 0.267 rg
BT 26.250 651.342 Td /F1 9.8 Tf  [(FLAHAULT A, DE LAMBALLERIE X, Hanslik T, salez n. Symptomatic infections less frequent with H1N1pdm than with )] TJ ET
BT 26.250 639.438 Td /F1 9.8 Tf  [(seasonal strains. PLOS Currents Influenza. 2009 Dec 27 . Edition 1. doi: 10.1371/currents.RRN1140.)] TJ ET
q
15.000 25.473 577.500 611.584 re W n
0.271 0.267 0.267 rg
BT 26.250 610.335 Td /F4 12.0 Tf  [(Abstract)] TJ ET
BT 26.250 590.381 Td /F1 9.8 Tf  [(A serosurvey conducted in a sample of first quarter pregnant women in France at week 48-49 of 2009 exhibit a seroprevalence )] TJ ET
BT 26.250 578.476 Td /F1 9.8 Tf  [(level of 10.6%. It has been extrapolated in male and female population living in France mainland, aged 20-39 yr, that 1,712,000, )] TJ ET
BT 26.250 566.571 Td /F1 9.8 Tf  [(95%CI \(1,112,700 � 2,311,300\) people were recently infected by H1N1pdm \(recently vaccinated women were excluded from )] TJ ET
BT 26.250 554.667 Td /F1 9.8 Tf  [(analysis\). From week 36 to 46-47 of 2009, 336,288, 95%CI \(207,303-421,299\) patients visited their general practitioners with )] TJ ET
BT 26.250 542.762 Td /F1 9.8 Tf  [(clinical influenza in France, mainland. We then extrapolated the proportion of symptomatic H1N1pdm influenza in both males )] TJ ET
BT 26.250 530.857 Td /F1 9.8 Tf  [(and females aged 20-39 yr who visited their GP to be 19.6%. Surprisingly, 8 months after having alerted at a global level for )] TJ ET
BT 26.250 518.952 Td /F1 9.8 Tf  [(H1N1pdm, there is still no published data on seroprevalence in any countries. Therefore, there is still no published estimation of )] TJ ET
BT 26.250 507.048 Td /F1 9.8 Tf  [(the proportion of asymptomatic infections due to the pandemic strain. We have launched in France a weekly repeated )] TJ ET
BT 26.250 495.143 Td /F1 9.8 Tf  [(seroprevalence survey in pregnant women who were volunteers to give their blood for H1N1pdm hemagglutination inhibition )] TJ ET
BT 26.250 483.238 Td /F1 9.8 Tf  [(\(HI\) serosurvey during their first term of their pregnancy [1] . By comparing our data to cumulated figures of incident cases of )] TJ ET
BT 26.250 471.333 Td /F1 9.8 Tf  [(clinical influenza provided by the French Sentinelles system [2] we provide estimate of the proportion of patients with )] TJ ET
BT 26.250 459.429 Td /F1 9.8 Tf  [(symptomatic influenza presentation which led them to visiting their general practitioner.)] TJ ET
BT 26.250 422.826 Td /F4 12.0 Tf  [(Funding Statement)] TJ ET
BT 26.250 402.872 Td /F1 9.8 Tf  [(This work is funded by EHESP School of Public Health, Rennes, France.)] TJ ET
BT 26.250 373.769 Td /F4 12.0 Tf  [(Weekly repeated H1N1pdm seroprevalence survey)] TJ ET
BT 26.250 353.815 Td /F1 9.8 Tf  [(We have launched a regular seroprevalence survey among a sample of consecutive pregnant women who systematically visit a )] TJ ET
BT 26.250 341.910 Td /F1 9.8 Tf  [(laboratory from a predefined network of labs for giving their blood for toxoplasmosis serology. These women gave a written )] TJ ET
BT 26.250 330.006 Td /F1 9.8 Tf  [(consent for providing a 50 microlitres serum sample for H1N1pdm serosurvey. Data on age and recent exposure to H1N1pdm )] TJ ET
BT 26.250 318.101 Td /F1 9.8 Tf  [(vaccination were collected. Blood samples were then shipped to Marseilles by express mail, where a standard HI assay using )] TJ ET
BT 26.250 306.196 Td /F1 9.8 Tf  [(human group O erythrocytes and 4 HI units of non inactivated H1N1pdm virus was performed in a L3 laboratory. Based on )] TJ ET
BT 26.250 294.291 Td /F1 9.8 Tf  [(results of previous tests performed in a sample of the general population and on the follow up of microbiologically documented )] TJ ET
BT 26.250 282.387 Td /F1 9.8 Tf  [(infections \(manuscript in preparation\), only dilution above 1/40 were considered to be relevant for detection of recent H1N1pdm )] TJ ET
BT 26.250 270.482 Td /F1 9.8 Tf  [(infections in this age group. From serological results obtained at weeks 48-49 in pregnant women, a cumulative seroprevalence )] TJ ET
BT 26.250 258.577 Td /F1 9.8 Tf  [(in both males and females aged 20-39 yr was estimated: 1,712,000 recent seroconversions to H1N1pdm, 95% confidence )] TJ ET
BT 26.250 246.672 Td /F1 9.8 Tf  [(interval \(1,112,700 ; 2,311,300\). These estimates are published on the InVS weekly bulletin )] TJ ET
0.267 0.267 0.267 rg
BT 422.412 246.672 Td /F1 9.8 Tf  [([3])] TJ ET
0.271 0.267 0.267 rg
BT 433.254 246.672 Td /F1 9.8 Tf  [(. Importantly, it should be noted )] TJ ET
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0.271 0.267 0.267 rg
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BT 26.250 767.476 Td /F1 9.8 Tf  [(cases suspected to be due to H1N1pdm, 336,288 cases,95% confidence interval \(207,303 ; 421,299\).)] TJ ET
BT 26.250 730.874 Td /F4 12.0 Tf  [(Calculation of the fraction of symptomatic H1N1pdm infections who visited their general )] TJ ET
BT 26.250 716.222 Td /F4 12.0 Tf  [(practitioners in France among the total number of infections in the age group 20-39 yr)] TJ ET
BT 26.250 696.267 Td /F1 9.8 Tf  [(Basis for calculation of this fraction was the following formula:)] TJ ET
BT 26.250 676.863 Td /F1 9.8 Tf  [([1 � {\(SeroprevH1N1-ClinicalH1N1\)/SeroprevH1N1}])] TJ ET
BT 26.250 657.458 Td /F1 9.8 Tf  [(We then extrapolated the proportion of symptomatic H1N1pdm influenza in French people aged 20-39 yr who visited their GP to )] TJ ET
BT 26.250 645.553 Td /F1 9.8 Tf  [(be 19.6%. This calculation may approach highest estimation of proportion of asymptomatic infections \(i.e. 80.4%\), as cases not )] TJ ET
BT 26.250 633.648 Td /F1 9.8 Tf  [(seen by general practitioners may not only be asymptomatic cases, but also paucisymptomatic cases or symptomatic cases )] TJ ET
BT 26.250 621.744 Td /F1 9.8 Tf  [(who did not seek any medical advice.)] TJ ET
BT 26.250 585.141 Td /F4 12.0 Tf  [(Competing Interests)] TJ ET
BT 26.250 565.187 Td /F1 9.8 Tf  [(The authors have declared that no competing interests exist.)] TJ ET
BT 26.250 528.584 Td /F4 12.0 Tf  [(Acknowledgements)] TJ ET
BT 26.250 508.630 Td /F1 9.8 Tf  [(Seroprevalence surveys were conducted by Unit� des Virus Emergents, Universit� Aix-Marseille 2, facult� de M�decine de )] TJ ET
BT 26.250 496.725 Td /F1 9.8 Tf  [(Marseille ; EHESP, Rennes, France ; R�seau de Biologie Mol�culaire Lib�rale ; Alliance nationale pour les Sciences de la Vie )] TJ ET
BT 26.250 484.821 Td /F1 9.8 Tf  [(et de la Sant�, France ; Institut de Veille Sanitaire, Saint-Maurice, France.)] TJ ET
BT 26.250 455.718 Td /F4 12.0 Tf  [(References)] TJ ET
BT 26.250 428.264 Td /F1 9.8 Tf  [(1.)] TJ ET
BT 38.132 428.264 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/docs_professionnels/methodo_surveillance_grippe_161209.pdf)] TJ ET
BT 26.250 408.859 Td /F1 9.8 Tf  [(2.)] TJ ET
BT 38.132 408.859 Td /F1 9.8 Tf  [(Carrat F, Flahault A, Boussard E, Farran N, Dangoumau L, Valleron AJ. Surveillance of influenza-like illness in France. The )] TJ ET
BT 26.250 396.954 Td /F1 9.8 Tf  [(example of the 1995/1996 epidemic. J Epidemiol Community Health. 1998 Apr;52 Suppl 1:32S-38S.)] TJ ET
BT 26.250 377.550 Td /F1 9.8 Tf  [(3.)] TJ ET
BT 26.250 365.645 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/points_h1n1/grippe_A_h1n1_151209/Bulletin_grippe_15_12_09.pdf#page=2)] TJ ET
BT 26.250 346.240 Td /F1 9.8 Tf  [(4.)] TJ ET
BT 38.132 346.240 Td /F1 9.8 Tf  [(http://www.sentiweb.org)] TJ ET
Q
q
15.000 328.859 577.500 448.141 re W n
0.271 0.267 0.267 rg
BT 26.250 767.476 Td /F1 9.8 Tf  [(cases suspected to be due to H1N1pdm, 336,288 cases,95% confidence interval \(207,303 ; 421,299\).)] TJ ET
BT 26.250 730.874 Td /F4 12.0 Tf  [(Calculation of the fraction of symptomatic H1N1pdm infections who visited their general )] TJ ET
BT 26.250 716.222 Td /F4 12.0 Tf  [(practitioners in France among the total number of infections in the age group 20-39 yr)] TJ ET
BT 26.250 696.267 Td /F1 9.8 Tf  [(Basis for calculation of this fraction was the following formula:)] TJ ET
BT 26.250 676.863 Td /F1 9.8 Tf  [([1 � {\(SeroprevH1N1-ClinicalH1N1\)/SeroprevH1N1}])] TJ ET
BT 26.250 657.458 Td /F1 9.8 Tf  [(We then extrapolated the proportion of symptomatic H1N1pdm influenza in French people aged 20-39 yr who visited their GP to )] TJ ET
BT 26.250 645.553 Td /F1 9.8 Tf  [(be 19.6%. This calculation may approach highest estimation of proportion of asymptomatic infections \(i.e. 80.4%\), as cases not )] TJ ET
BT 26.250 633.648 Td /F1 9.8 Tf  [(seen by general practitioners may not only be asymptomatic cases, but also paucisymptomatic cases or symptomatic cases )] TJ ET
BT 26.250 621.744 Td /F1 9.8 Tf  [(who did not seek any medical advice.)] TJ ET
BT 26.250 585.141 Td /F4 12.0 Tf  [(Competing Interests)] TJ ET
BT 26.250 565.187 Td /F1 9.8 Tf  [(The authors have declared that no competing interests exist.)] TJ ET
BT 26.250 528.584 Td /F4 12.0 Tf  [(Acknowledgements)] TJ ET
BT 26.250 508.630 Td /F1 9.8 Tf  [(Seroprevalence surveys were conducted by Unit� des Virus Emergents, Universit� Aix-Marseille 2, facult� de M�decine de )] TJ ET
BT 26.250 496.725 Td /F1 9.8 Tf  [(Marseille ; EHESP, Rennes, France ; R�seau de Biologie Mol�culaire Lib�rale ; Alliance nationale pour les Sciences de la Vie )] TJ ET
BT 26.250 484.821 Td /F1 9.8 Tf  [(et de la Sant�, France ; Institut de Veille Sanitaire, Saint-Maurice, France.)] TJ ET
BT 26.250 455.718 Td /F4 12.0 Tf  [(References)] TJ ET
BT 26.250 428.264 Td /F1 9.8 Tf  [(1.)] TJ ET
BT 38.132 428.264 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/docs_professionnels/methodo_surveillance_grippe_161209.pdf)] TJ ET
BT 26.250 408.859 Td /F1 9.8 Tf  [(2.)] TJ ET
BT 38.132 408.859 Td /F1 9.8 Tf  [(Carrat F, Flahault A, Boussard E, Farran N, Dangoumau L, Valleron AJ. Surveillance of influenza-like illness in France. The )] TJ ET
BT 26.250 396.954 Td /F1 9.8 Tf  [(example of the 1995/1996 epidemic. J Epidemiol Community Health. 1998 Apr;52 Suppl 1:32S-38S.)] TJ ET
BT 26.250 377.550 Td /F1 9.8 Tf  [(3.)] TJ ET
BT 26.250 365.645 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/points_h1n1/grippe_A_h1n1_151209/Bulletin_grippe_15_12_09.pdf#page=2)] TJ ET
BT 26.250 346.240 Td /F1 9.8 Tf  [(4.)] TJ ET
BT 38.132 346.240 Td /F1 9.8 Tf  [(http://www.sentiweb.org)] TJ ET
Q
q
15.000 328.859 577.500 448.141 re W n
0.271 0.267 0.267 rg
BT 26.250 767.476 Td /F1 9.8 Tf  [(cases suspected to be due to H1N1pdm, 336,288 cases,95% confidence interval \(207,303 ; 421,299\).)] TJ ET
BT 26.250 730.874 Td /F4 12.0 Tf  [(Calculation of the fraction of symptomatic H1N1pdm infections who visited their general )] TJ ET
BT 26.250 716.222 Td /F4 12.0 Tf  [(practitioners in France among the total number of infections in the age group 20-39 yr)] TJ ET
BT 26.250 696.267 Td /F1 9.8 Tf  [(Basis for calculation of this fraction was the following formula:)] TJ ET
BT 26.250 676.863 Td /F1 9.8 Tf  [([1 � {\(SeroprevH1N1-ClinicalH1N1\)/SeroprevH1N1}])] TJ ET
BT 26.250 657.458 Td /F1 9.8 Tf  [(We then extrapolated the proportion of symptomatic H1N1pdm influenza in French people aged 20-39 yr who visited their GP to )] TJ ET
BT 26.250 645.553 Td /F1 9.8 Tf  [(be 19.6%. This calculation may approach highest estimation of proportion of asymptomatic infections \(i.e. 80.4%\), as cases not )] TJ ET
BT 26.250 633.648 Td /F1 9.8 Tf  [(seen by general practitioners may not only be asymptomatic cases, but also paucisymptomatic cases or symptomatic cases )] TJ ET
BT 26.250 621.744 Td /F1 9.8 Tf  [(who did not seek any medical advice.)] TJ ET
BT 26.250 585.141 Td /F4 12.0 Tf  [(Competing Interests)] TJ ET
BT 26.250 565.187 Td /F1 9.8 Tf  [(The authors have declared that no competing interests exist.)] TJ ET
BT 26.250 528.584 Td /F4 12.0 Tf  [(Acknowledgements)] TJ ET
BT 26.250 508.630 Td /F1 9.8 Tf  [(Seroprevalence surveys were conducted by Unit� des Virus Emergents, Universit� Aix-Marseille 2, facult� de M�decine de )] TJ ET
BT 26.250 496.725 Td /F1 9.8 Tf  [(Marseille ; EHESP, Rennes, France ; R�seau de Biologie Mol�culaire Lib�rale ; Alliance nationale pour les Sciences de la Vie )] TJ ET
BT 26.250 484.821 Td /F1 9.8 Tf  [(et de la Sant�, France ; Institut de Veille Sanitaire, Saint-Maurice, France.)] TJ ET
BT 26.250 455.718 Td /F4 12.0 Tf  [(References)] TJ ET
BT 26.250 428.264 Td /F1 9.8 Tf  [(1.)] TJ ET
BT 38.132 428.264 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/docs_professionnels/methodo_surveillance_grippe_161209.pdf)] TJ ET
BT 26.250 408.859 Td /F1 9.8 Tf  [(2.)] TJ ET
BT 38.132 408.859 Td /F1 9.8 Tf  [(Carrat F, Flahault A, Boussard E, Farran N, Dangoumau L, Valleron AJ. Surveillance of influenza-like illness in France. The )] TJ ET
BT 26.250 396.954 Td /F1 9.8 Tf  [(example of the 1995/1996 epidemic. J Epidemiol Community Health. 1998 Apr;52 Suppl 1:32S-38S.)] TJ ET
BT 26.250 377.550 Td /F1 9.8 Tf  [(3.)] TJ ET
BT 26.250 365.645 Td /F1 9.8 Tf  [(http://www.invs.sante.fr/surveillance/grippe_dossier/points_h1n1/grippe_A_h1n1_151209/Bulletin_grippe_15_12_09.pdf#page=2)] TJ ET
BT 26.250 346.240 Td /F1 9.8 Tf  [(4.)] TJ ET
BT 38.132 346.240 Td /F1 9.8 Tf  [(http://www.sentiweb.org)] TJ ET
Q
q
0.000 0.000 0.000 rg
BT 291.710 19.825 Td /F1 11.0 Tf  [(2)] TJ ET
BT 25.000 19.825 Td /F1 11.0 Tf  [(PLOS Currents Influenza)] TJ ET

Q
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