Fruit bats of the genus Pteropus occur throughout the Austral-Asian region west to islands off the eastern coast of Africa. Recent phylogenetic analyses of Pteropus from the western Indian Ocean found low sequence divergence and poor phylogenetic resolution among several morphologically defined species. We reexamine the phylogenetic relationships of these taxa by using multiple individuals per species. In addition, we estimate population genetic structure in two well-sampled taxa occurring on Madagascar and the Comoro Islands (P. rufus and P. seychellensis comorensis). Despite finding a similar pattern of low sequence divergence among species, increased sampling provides insight into the phylogeographic history of western Indian Ocean Pteropus, uncovering high levels of gene flow within species.
Flying foxes, belonging to the family Pteropodidae (Genus Pteropus), are species rich with 65 recognized species primarily distributed from Australia, westwards though tropical and subtropical Asia, to islands in the western Indian Ocean and offshore eastern Africa . All of these species have been described based on morphological characters, including size, pelage coloration, and cranio-dental characters. Resolving the phylogenetic relationships among species, typifying patterns of gene flow within members of the genus, and identifying instances of hybridization among species is important both for taxonomy and phylogeography; these aspects in turn provide insights for conservation. Fruit bats, including Pteropus, have been identified as reservoirs for pathogens known to cause zoonotic diseases in humans   ; Pteropus bats come into close contact with human populations through their utilization as bush-meat and the consumption of fruits from the same trees . Data on connectivity within and among populations are critical for understanding aspects of disease transmission.
Certain volant mammals, including members of the genus Pteropus, are capable of long distance dispersal . Several Pteropus species are known to travel up to 50 km per night between day roosts and feeding sites and they are capable of traversing considerable expanses across open water (e.g.,  ). For example, based on satellite telemetry, P. vampyrus is notably mobile traveling hundreds of kilometers between day-roost sites during the course of a year, and crossing international borders . Past studies have found varying levels of intraspecific genetic differentiation among island populations of pteropodids based on mitochondrial sequences and allozyme data. For some members of the genus Pteropus, there is considerable gene flow within species across broad geographical areas, but very little differentiation between subspecies or sister species   . In contrast, other genera of Pteropodidae from southwest Pacific Ocean islands tend to show greater levels of intraspecific genetic structure  .
There are eight recognized species of Pteropus on offshore islands along the coast of eastern Africa and in the western Indian Ocean, including Madagascar and the Mascarene, Seychelles, and Comoro archipelagos (Figure 1) .
Many Pteropus taxa are restricted to a single island or portion of an archipelago    and are diagnosed based on morphological characters. For example, P. seychellensis comorensis from the Comoros are considered to be closely related to P. s. seychellensis on the distant Seychelles based on size and pelage , rather than to the more geographically proximate P. rufus in Madagascar (Figure 1).
Recently, O’Brien et al.  investigated the molecular systematics and associated phylogeography of Pteropus from the Indian Ocean. They found strong support for clades within Pteropus, including the resolution of a clade containing P. rufus from Madagascar, the subspecies P. s. seychellensis and P. s. comorensis, P. aldabrensis from Aldabra Atoll (extreme western Seychelles)and P. niger from Mauritius (Figure 1). However, within this clade, there was low support for the monophyly of any of these species and for the relationships among these taxa (Figure 2).
This suggests that there may be recent or current gene flow among species and calls into question the current taxonomy of regional members of this genus. While sampling within this clade was limited, P. s. seychellensis and P. s. comorensis were not recovered as sister taxa. Instead, P. s. seychellensis was strongly supported as sister to P. niger (constrained to be monophyletic in their analyses) and this clade formed an unresolved polytomy with multiple samples of P. s. comorensis and single representatives of both P. rufus , and P. aldabrensis.
Here, we re-evaluate the phylogeographic history and estimate the population genetic structure of western Indian Ocean Pteropus. Our aims are twofold. First, we investigate whether increased sampling helps to clarify species limits and elucidate phylogenetic relationships among P. rufus, P. s. comorenesis, P. s. seychellensis, P. niger,and P. aldabrensis. Second, we use population sampling to examine patterns of genetic connectivity within P. rufus on Madagascar and P. s. comorensis on the Comoros.
Materials and Methods
Ear clips were collected in the field from released individuals in the context of a research program of the Institut Pasteur de Madagascar and preserved in EDTA. Additional tissue samples associated with specimens housed in the Field Museum of Natural History and from captive animals at the Lubee Bat Conservancy were also included (Table 1).
Table 1. Newly sequenced samples included in this study. Abbreviations as follows. CS: Institut Pasteur; Chauve-souris; SMG: Steven M. Goodman; ISIS: Lubee Foundation; AJ, AJL, R, H, GC, MH, MT, and V: Lubee Bat Conservancy band identification numbers.
|Pteropus giganteus||ISIS930045||Lubee Bat Conservancy, Captive Born|
|Pteropus hypomelanus||H10||Lubee Bat Conservancy|
|Pteropus livingstonii||AJL07-11, AJL14-17, AJL19||Comoro Islands, Anjouan|
|Pteropus poliocephalus||ISIS930315, ISIS930139-930141, ISIS930349, ISIS930372, ISIS930375||Lubee Bat Conservancy, Captive Born|
|Pteropus rodricensis||R20-21||Lubee Bat Conservancy|
|Pteropus rufus||CS117-126||Madagascar: Province de Toliara, Boromena, Miandrivazo|
|CS132-137, CS141-142||Madagascar: Province de Toliara, Ankotrofotsy, Miandrivazo|
|CS230, CS285, CS516, CS518, CS521-522||Madagascar: Province de Fianarantsoa, Vangaindrano|
|SMG14638-14647||Madagascar: Province de Mahajanga, near Maroadabo|
|SMG14737-14746||Madagascar: Province de Mahajanga, near Andakalaka|
|SMG15042-15044||Madagascar: Province d’Antsiranana, Nosy Be|
|SMG15295-15304||Madagascar: Province de Toamasina, Isle Sainte Marie|
|Pteropus seychellensis comorensis||AJ05, AJ08, AJ23-27, AJ28A, AJ42, AJ44||Comoro Islands, Anjouan|
|GC06a,GC07a, GC27a-29a, GC48a, GC50a, GC05b, GC08b||Comoro Islands, Grande Comore|
|MH04a, MH06, MH07a-08a, MH27-28, MH35, MH37, MH41-42||Comoro Islands, Mohéli|
|MT02a-05a, MT10-14, MT23||Comoro Islands, Mayotte|
|Pteropus vampyrus||V7||Lubee Bat Conservancy|
Genomic DNA was extracted from a small tissue sample using either the Nucleospin DNA Extraction Kit or the Qiagen DNeasy Tissue Kit. The mitochondrial gene, cytochrome-b (cyt- b ), was amplified using the primers L14724 and H15915 . PCR reactions were conducted in a total volume of 25 µL including 1 x Buffer (100 mM Tris-HCL, pH 8.3, 500 mM KCl), 2 mM MgCl 2 , 1 mM dNTP, 0.25 µM of each primer, 0.5 U Taq polymerase, and 1 µL template DNA. PCR cycles consisted of an initial denaturation of 94 °C for 2 min, 35 cycles of 94 °C denaturation for 1 min, 48 °C annealing for 1 min, and 72 °C extension for 1.5 min, and a final extension of 10 min at 72 °C. Five µL of amplified product were incubated with 0.4 µL ExoSapIT (USB) and 1.6 µL water at 37 °C for 15 minutes followed by 80 °C for 15 minutes. Cleaned PCR products were sequenced using the primers used for amplification and an internal primer when necessary (PterCytbInt1 5’ GGRGCAACAGTCATYACYAA 3’) in a total volume of 5 µL (1 x buffer, 1 µM primer, 0.2 µL BigDye v3, and 1.0 µL cleaned PCR product). Sequencing reactions were run on an ABI 3730xl DNA Analyzer capillary machine and sequences were checked by eye and assembled into contigs in Sequencher 4.8 (GeneCodes, Ann Arbor, Michigan).
We supplemented these data with sequences from GenBank (see Table S1) and used MacClade  to align sequences by hand and check for stop codons. All sequences have been deposited in GenBank (JF327207 – JF327326). We reconstructed the phylogenetic relationships among DNA sequences under maximum parsimony (MP), maximum likelihood (ML), and Bayesian inference using other Pteropodidae (Ptenochirusjagori and Cynopterus brachyotis) as outgroups to root phylogenies based on previous phylogenetic work    . Maximum parsimony bootstrap analyses were conducted in PAUP 4b10; we used 1,000 bootstrap replicates of the fast-search option.
We determined the model of sequence evolution that best fit the entire data set and each partition of the data (first, second, and third codon positions) using MrModeltest 2.3  . One hundred ML bootstrap replicates were conducted in Garli 1.0 under a GTR+I.+G model of sequence evolution. Partitioned Bayesian phylogenetic analyses were performed in MrBayes 3.1.2  . These consisted of three independent runs each with four incrementally heated chains sampled every 1,000 generations for 10 million generations. We verified adequate mixing within runs and convergence among runs in Tracer and discarded the first 1,001 samples from each run before summarizing trees.
To examine phylogeographic structure within species, we constructed a haplotype network within a parsimony framework in TCS 1.21 . We included all descendants of the most recent common ancestor for P. rufus and P. s. comorensis that were well-supported in the Bayesian phylogenetic inference. We used a 95% connection limit and treated gaps as a fifth state.
We assessed whether genetic diversity was best explained by within population variation or among population variation using an AMOVA executed in Arlequin 3.5 . Samples of P . s . comorensis from the Comoros were grouped by island (Grande Comore, Mohéli, Anjouan, and Mayotte) and P. rufus from Madagascar were separated into five groups based on the proximity of collection localities (Figure 1).
The data matrix and trees have been submitted to Treebase under study number 12080 ( http://treebase.org/treebase-web/search/study/summary.html?id=12080 ).
The final cyt- b sequence alignment consisted of 1,123 base pairs for 167 individuals. Across all samples, including outgroups, we found 648 invariable and 375 parsimony informative sites. Among Pteropus sequences, 755 sites were invariable and 294 were parsimony informative. We found 33 unique haplotypes among P. rufus and 12 unique haplotypes among P. s. comorensis.
Phylogenetic analyses of cyt- b sequence data using three optimality criteria were largely congruent at deeper nodes, but support values for relationships within Pteropus were not always consistent across analyses. Pteropus was resolved as a monophyletic group with low support (Figure 3).
We found moderate to strong support for the monophyly for a group containing all Pteropus except P. scapulatus (Figure 3A). Among western Indian Ocean taxa, our data support the sister species relationship between P. livingstonii from Anjouan (Comoros), and P. voeltzkowi, from the Pemba Island (Tanzania) (Figure 1). There was little resolution within a clade containing four species of western Indian Ocean Pteropus (P. aldabrensis, P. niger, P. rufus, and P. seychellensis) (Figure 3B). We did not find any support for the monophyly of P. rufus (MP=0; ML=0; BP=0)and very weak support for the monophyly of P. s. comorensis ( MP=23; ML=0; BP=0.16). Moreover, there was no support for a sister pair relationship between the two races of P. seychellensis (MP=0; ML=0; BP=0), but we did find strong support for a clade comprising P. s. seychellensis and P. niger.
Mitochondrial haplotypes from each species clustered together in the haplotype network, but P. s. comorensis and P. rufus alleles differed by as few as two base-pairs (Figure 4). Based on this haplotype network, P. s. seychellensis was more closely related to P. niger and together, were more closely related to P. rufus than to P. s . comorensis.
We found evidence for population genetic structure in P.s. comorensis and P. rufus , although values of ϕ ST and F ST were low. For P.s. comorensis, overall ϕ ST was 0.0676 and was significant at p = 0.0423; however, in pairwise population tests of differentiation, only one pair of islands had an F ST significantly greater than zero (Table 2). In contrast overall ϕ ST for P. rufus was 0.130 (p < 0.001). Three pairwise population F ST values were significantly different from zero, but these did not show a clear trend with geographic distance (Table 3).
Table 2. F ST for P . seychellensis comorensis. Estimates of F ST significantly different from zero are indicated in bold.
Table 3. F ST for Pteropus rufus. Estimates of F ST significantly different from zero are indicated in bold.
|Mahajanga||Nosy Be||Ile Ste Marie||Miandrivazo|
|Ile St Marie||0.05057||-0.09239|
By sampling multiple individuals per species, we are able confirm the monophyly of several taxa where sampling was previously limited , including P. hypomelanus, P. poliocephalus, P. livingstonii, P. rodricensis, P. vampyrus, and P. giganteus (Figure 3). However, increased sampling did not help to resolve the interspecific relationships at deeper nodes in the phylogeny, and in this sense, have not improved upon those of O’Brien et al. . In general, the phylogenetic relationships among Pteropus species were more strongly supported in Bayesian analyses than in MP or ML analyses. A monophyletic group containing four western Indian Ocean species (P. rufus, P. niger, P. aldabrensis, and P. seychellensis) was suggested by Bayesian inference, but did not receive strong support in either MP or ML analyses (Figure 3). Within this group, we found support for the monophyly of P. s. seychellensis, but not for any other species.
Population sampling for P. rufus and P. s. comorensis confirms that interspecific sequence divergence is low and additionally suggests that P. rufus may be paraphyletic with respect to P. s. comorensis and P. aldabrensis. We did not find support for the monophyly of either P. rufus or P. s. comorensis and recovered weak support for a clade containing all P. s. comorensis haplotypes and one P. rufus haplotype from Miandrivazo (Figure 1, 3). It is possible that this phylogenetic pattern simply reflects a lack of resolution due to low mitochondrial sequence divergence or incomplete lineage sorting. However, if the P. rufus individual is truly most closely related to P. s. comorensis, migration from the Comoros to Madagascar with subsequent introgression may be an alternative explanation for this pattern.
We included multiple samples of P. niger but in constrast to O’Brien et al.  we did not constrain them to be monophyletic. While we also found that P. s. seychellensis and P. niger are most closely related to one another and that P. s. seychellensis is not sister to P. s. comorensis, we were unable to determine whether the former two taxa are reciprocally monophyletic and the position of these two species relative to P. rufus, P. s. comorensis, and P. aldabrensis remains unclear. Our results place, P. niger – P. s. seychellensis as part of a larger polytomy that includes the latter three species (Figure 3), but these haplotypes are most closely related to P. rufus in parsimony networks (Figure 4).
Low sequence divergence within this clade of western Indian Ocean Pteropus suggests that these species diverged very recently. The island of Madagascar is at least 80 ma, considerably older than the islands and archipelagos of Mauritius, Aldabra, Comoros, and the Seychelles; certain in situ volcanic islands, such as Grande Comore, were formed no more than 0.5 ma  . It is therefore reasonable to hypothesize that the island of Madagascar held large and stable populations of Pteropus that could have served as the source population for these smaller islands. Under this scenario, P. aldabrensis, P. niger, P. s. seychellensis, and P. s. comorensis would sensibly be nested within the P. rufus clade.
While P. s. comorensis and P. rufus are distinct taxa on the basis of morphological characteristics , our population-level sampling suggests a more recent shared history than previously hypothesized. Pteropus s. comorensis and P. rufus may have evolutionary histories more similar to that found within smaller bodied western Indian Ocean bats. Several species of bats occur in both the Comoro archipelago and Madagascar, over 300 km away . For two small bodied taxa, Mops leucostigma and Chaerephon leucogaster, there is only weak mitochondrial divergence between the regions  . In contrast, two sister species of fruit bats in the genus Rousettus from Madagascar (R. madagascariensis) and the Comoros (R. obliviosus) are deeply divergent from one another at both mitochondrial and nuclear loci .
Within each of the two species for which we have population level data, P. s. comorensis and P. rufus, we find only weak genetic differentiation. Among P. s. comorensis populations on the different islands within the Comoros, very little genetic diversity is explained by among island variation and F ST is significantly greater than zero for only one population pair of intermediate geographic distance. No clear pattern of genetic differentiation has been found for several other species of bats from these islands including a smaller bodied fruit bat, Rousettus oblivious , and the molossid bat Chaerephon pusillus. In contrast, a study of small bats of the genus Miniopterus from Anjouan and Grande Comore found much greater inter-island genetic divergence .
We found moderate differentiation for the Malagasy species, P. rufus; shared haplotypes across the five sampled regions on Madagascar suggest ongoing gene flow and results from the AMOVA indicate that despite some differentiation, genetic diversity is largely explained by within population variation rather than among population differences. Nonetheless, pairwise F ST values were significant for several population pairs (Table 3) and were notably high between Miandrivazo, in central Madagascar, and a more northern locality, Mahajanga, and also between Miandrivazo and the southeastern locality, Vangaindrano (Table 3). Miandrivazo may represent a geographically isolated region with corridors to the west and east connecting the other populations. While P. rufus do not appear to be panmictic across Madagascar, it is difficult to determine whether such patterns of differentiation are due to true barriers to gene flow or isolation by distance and genetic drift-gene flow equilibrium . Previously, taxonomists segregated P. rufus into different subspecies, specifically the recognition of P. r. princeps named from the Tolagnaro region in southeastern Madagascar  and about 185 km south of our Vangaindrano sampling site. On the basis of the phylogeographic data presented herein, there is no evidence of divergence in the southeastern populations of P. rufus.
Intraspecific patterns of genetic differentiation among other Malagasy bats vary considerably; some species are genetically panmictic throughout their distribution (e.g. R. madagascariensis; Triaenops rufus) whereas others show significant population genetic differentiation (e.g. Myzopoda aurita; T. furculus ). Compared to R. madagascariensis, the other Malagasy pteropodid studied to date, P. rufus appears to have greater population structure. While we only include mitochondrial data for P. rufus and have limited geographic sampling in comparison to the R. madagscariensis study , our data suggest that patterns of population connectivity are not identical in these two fruit bats.
The recent recolonization of P. niger on the Mascarene island of La Réunionindicates that there is considerable capacity of Pteropus bats in the western Indian Ocean to move among islands and across open expanses of water. Two species, P. niger and P. subniger, formerly inhabited this island but were extirpated by human hunting pressures . The former species was able to maintain populations on Mauritius, and after an absence of at least 200 years on La Réunion, it has recolonized (Jean-Michel Probst, pers. comm.), traversing the ca. 170 km between these two islands. It is conceivable that long distance dispersal among islands of the Comoro archipelago and Madagascar occurs with some regularity, providing the opportunity for inter-island exchange of zoonotic diseases. However, such dispersal events may be rarer among other western Indian Ocean Pteropus.
With the addition of multiple samples per species, it is clear that forms of western Indian Ocean Pteropus , as classically defined, have recently diverged from one another and that their species-level taxonomy may be in need of revision. Moreover, despite significant measures of intraspecific population genetic differentiation, Pteropus may be capable of long distance movements connecting populations across broad geographic distances. Including rapidly-evolving independent nuclear markers in future studies will undoubtedly provide insight into patterns of differentiation within and among species of Pteropus bats. Such data will guide sound taxonomic changes associated with the species limits of western Indian Ocean Pteropus and will also serve as an independent estimate of genetic connectivity that includes both maternal and paternal lineages.
On Madagascar, we are grateful to the Direction des Eaux et Forêts and Association National pour la Gestion des Aires Protégées, and in the Comoros, to Yahaya Ibrahim of the Centre National de Documentation et de Recherche Scientifique and Ishaka Saïd of Action Comores for aid in numerous ways, including permission to collect specimens. We acknowledge Eddy Rakotonandrasana, Fanja Ratrimomanarivo, Manuel Ruedi, and Nicole Weyeneth for their aid with fieldwork, and Jean-Marc Reynes and Catherine Iehlé, formerly of the Institut Pasteur de Madagascar, for the collection of the Pteropus ear clips. We thank Brian Pope at the Lubee Bat Conservancy for his assistance, Teresa Ai, and Jonathan Schwartz for help with molecular data collection, the Duke Institute for Genome Science and Policy, and the Duke Shared Cluster Resource.
Conservation International (CABS), John D. and Catherine T. MacArthur Foundation, and the Volkswagen Foundation have generously supported field research associated with this paper. NSF award DEB-0516276 to SMG and ADY supported associated molecular research.
The authors have declared that no competing interests exist.
Table S1. Cytochrome-b sequences and associated GenBank numbers. SM refers to Supplementary Materials in O’Brien et al. .
|Species||GenBank Accession Number|
|Pteropus seychellensis comorensis||FJ561395|
|Pteropus seychellensis seychellensis||FJ561399|
- Simmons NB (2005) Order Chiroptera. In: Wilson DE, Reeder DM, editors. Mammal species of the World: A taxonomic and geographic reference. 3rd ed. Baltimore: John Hopkins University Press. pp. 315-521.
- Epstein JH, Quan P-L, Briese T, Street C, Jabado O, et al. (2010) Identification of GBV-D, a novel GB-like flavivirus from Old World frugivorous bats (Pteropus giganteus) in Bangladesh. PLoS Pathogens 6: e1000972.
- Halpin K, Young PL, Field HE, Mackenzie JS (2000) Isolation of Hendra virus from pteropid bats: a natural reservoir of Hendra virus. Journal of General Virology 81: 1927-1932.
- Iehlé C, Razafitrimo G, Razainirina J, Andriaholinirina N, Goodman SM, et al. (2007) Henipavirus and Tioman virus antibodies in pteropodid bats, Madagascar. Emerging Infectious Diseases 13: 159-161.
- Luskin MS (2010) Flying Foxes prefer to forage in farmland in a tropical dry forest landscape mosaic in Fiji. Biotropica 42: 246-250.
- Ditchfield AD (2000) The comparative phylogeography of Neotropical mammals: patterns of intraspecific mitochondrial DNA variation among bats contrasted to nonvolant small mammals. Molecular Ecology 9: 1307-1318.
- Palmer C, Price O, Bach C (2000) Foraging ecology of the black flying fox (Pteropus alecto) in the seasonal tropics of the Northern Territory, Australia. Wildlife Research 27: 169-178.
- Tidemann CR, Nelson JE (2004) Long-distance movements of the grey-headed flying fox (Pteropus poliocephalus). Journal of Zoology 263: 141-146.
- Epstein JH, Olival KJ, Pulliam JRC, Smith C, Westrum J, et al. (2009) Pteropus vampyrus, a hunted migratory species with a multinational home‐range and a need for regional management. Journal of Applied Ecology 46: 991-1002.
- Ingleby S, Colgan D (2003) Electrophoretic studies of the systematic and biogeographic relationships of the Fijian bat genera Pteropus, Pteralopex, Chaerephon and Notopteris. Australian Mammalogy 25: 13-29.
- Webb NJ, Tidemann CR (1996) Mobility of Australian flying-foxes, Pteropus spp. (Megachiroptera): evidence from genetic variation. Proceedings of the Royal Society B 263: 497-502.
- Sinclair EA, Webb NJ, Marchant AD, Tidemann CR (1996) Genetic variation in the little red flying-fox Pteropus scapulatus (Chiroptera: Pteropodidae): Implications for management. Biological Conservation 76: 45-50.
- Roberts TE (2006) History, ocean channels, and distance determine phylogeographic patterns in three widespread Philippine fruit bats (Pteropodidae). Molecular Ecology 15: 2183-2199.
- Goodman SM, Weyeneth N, Ibrahim Y, Saïd I, Ruedi M (2010) A review of the bat fauna of the Comoro Archipelago. Acta Chiropterologica 12: 117-141.
- O'Brien J, Mariani C, Olson L, Russell AL, Say L, et al. (2009) Multiple colonisations of the western Indian Ocean by Pteropus fruit bats (Megachiroptera: Pteropodidae): the furthest islands were colonised first. Molecular Phylogenetics and Evolution 51: 294-303.
- Goodman SM, Andriafidison D, Andrianaivoarivelo R, Cardiff SG, Ifticene E, et al. (2005) The distribution and conservation of bats in the dry regions of Madagascar. Animal Conservation 8: 153-165.
- Irwin DM, Kocher TD, Wilson AC (1991) Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution 32: 128-144.
- Maddison D, Maddison W (2003) MacClade 4: analysis of phylogeny and character evolution. Version 4.06.
- Agnarsson I, Zambrana-Torrelio CM, Flores-Saldana NP, May-Collado LJ (2011) A time-calibrated species-level phylogeny of bats (Chiroptera, Mammalia). PLoS Currents: Tree of Life.
- Colgan DJ, da Costa P (2002) Megachiropteran evolution studied with 12S rDNA and c-mos DNA sequences. Journal of Mammalian Evolution 9: 3-22.
- Jones KE, Purvis A, MacLarnon A, Bininda-Emonds OR, Simmons NB (2002) A phylogenetic supertree of the bats (Mammalia: Chiroptera). Biological Reviews 77: 223-259.
- Giannini NP, Almeida FC, Simmons NB, Helgen KM (2008) The systematic position of Pteropus leucopterus and its bearing on the monophyly and relationships of Pteropus (Chiroptera: Pteropodidae). Acta Chiropterologica 10: 11-20.
- Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817-818.
- Nylander J (2004) MrModeltest v2. Program distributed by the author.
- Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754-755.
- Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
- Clement M, Posada D, Crandall K (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology 9: 1657-1660.
- Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10: 564-567.
- Gillot P-Y, Lefèvre J-C, Nativel P-E (1994) Model for the structural evolution of the volcanoes of Réunion Island. Earth and Planetary Science Letters 122: 291-302.
- Nougier J, Cantagrel JM, Karche JP (1986) The Comores archipelago in the western Indian Ocean: volcanology, geochronology and geodynamic setting. Journal of African Earth Sciences 5: 135-145.
- Ratrimomanarivo FH, Goodman SM, Hoosen N, Taylor PJ, Lamb J (2009) Morphological and molecular variation in Mops leucostigma (Chiroptera: Molossidae) of Madagascar and the Comoros: phylogeny, phylogeography, and geographic variation. Mitteilungen Hamburgisches Zoologisches Museum und Institut 105: 57-101.
- Ratrimomanarivo FH, Goodman SM, Stanley WT, Naidoo T, Taylor PJ, et al. (2009) Geographic and phylogeographic variation in Chaerephon leucogaster (Chiroptera: Molossidae) of Madagascar and the western Indian Ocean islands of Mayotte and Pemba. Acta Chiropterologica 11: 25-52.
- Goodman SM, Chan LM, Nowak MD, Yoder AD (2010) Phylogeny and biogeography of western Indian Ocean Rousettus (Chiroptera: Pteropodidae). Journal of Mammalogy 91: 593-606.
- Goodman SM, Buccas W, Naidoo T, Ratrimomanarivo F, Taylor PJ, et al. (2010) Patterns of morphological and genetic variation in western Indian Ocean members of the Chaerephon 'pumilus' complex (Chiroptera: Molossidae), with the description of a new species from Madagascar. Zootaxa: 1-36.
- Weyeneth N, Goodman SM, Stanley WT, Ruedi M (2008) The biogeography of Miniopterus bats (Chiroptera: Miniopteridae) from the Comoro Archipelago inferred from mitochondrial DNA. Molecular Ecology 17: 5205-5219.
- Irwin DE (2002) Phylogeographic breaks without geographic barriers to gene flow. Evolution 56: 2383-2394.
- Peterson RL, Eger JL, Mitchell L (1995) Chiroptères. Faune de Madagascar 84: 1-204.
- Russell AL, Ranivo J, Palovacs EP, Goodman SM, Yoder AD (2007) Working at the interface of phylogentics and population genetics: a biogeographical analysis of Triaenops spp. (Chiroptera: Hipposideridae). Molecular Ecology 16: 839-851.
- Russell AL, Goodman SM, Fiorentino I, Yoder AD (2008) Population genetic analysis of Myzopoda (Chiroptera : Myzopodidae) in Madagascar. Journal of Mammalogy 89: 209-221.
- Cheke AS, Dahl JF (1981) The status of bats on western Indian Ocean islands, with special reference to Pteropus. Mammalia 45: 205-238.